07EA8DEB-ED66-4495-944A-01F7A59DFFB6REPOXYBLE: Depolymerizable bio-based multifunctional closed loop recyclable epoxy systems for energy efficient structuresActive10067645EU-FundedMaterials, especially advanced materials, are the backbone and source of prosperity of an industrial society” (Materials 2030 Manifesto). The Green Deal and the Digital Decade establish high-priority policies for Europe, where 70% of all technical innovations are directly or indirectly attributed to advanced materials. Lightweight and high-strength materials have consistently played a key role in the construction of fuel-efficient and high-performing transportation structures. Lightweight materials such as glass and carbon fibres composites are commonly used due to their intrinsic properties such as high mechanical performance. However, the poor recyclability and recovery aspect poses a significant challenge. The end-of-life aspect of these materials is crucial, as when landfilled they release toxic substances into the environment. Moreover, minimising resource use, energy of manufacturing processes and optimising waste disposal of future advanced materials can help mitigate cost and product’s end-to-end footprint acrossits global lifecycle, thereby significantly improving its overall environmental performance. REPOXYBLE will create a new class of high-performance materials -bio-based epoxy composites targeting cost and energy effectiveness, recyclability and sustainability. REPOXYBLE assumes an upstream approach more efficient and effective than having to address deficiencies at the end of the product development process. This approach integrates product performance, multifunctionality, sustainability, safety and potential legal concerns, while there is still time to act, on the monomers’ synthesis, the resin formulation and the future composite design. REPOXYBLE is driven by two complementary market applications in the aerospace and automotive sectors.2026-05-30240CEBFD-1052-4EAC-88DF-D88A163D61C8Horizon Europe Guarantee2022-12-01INCOME_ACTUAL410973100676456CFA1E1F-F25C-4C23-8FE1-C47AE53E333EUnclassified08985353-1E14-4F3D-9480-0020ACFD7CC2The Multimodal Translation of Intangible Cultural HeritageActive2930848StudentshipMotivation Intangible Cultural Heritage (ICH) represents the dynamic tapestry of human civilization, encapsulating our collective traditions and cultures. As highlighted by the 2003 UNESCO Convention on the Safeguarding of the ICH, preserving these diverse cultural manifestations is crucial. In the digital era, technological advancements such as transmedia, VR, AR, and 3D modeling allowed diverse forms of information to be digitally transformed and presented in increasingly vivid and comprehensive digital formats (Alivizatou-Baeakou, 2017; Rossau et al., 2019). While these innovations offer unique opportunities to enhance the representation of ICH, they also pose challenges in representing its dynamic, multimodal nature. Furthermore, current digitalisation methods tend to be technocentric and often overlook the cultural contexts and holistic nature of ICH, potentially leading to a loss of its evolving, living character (Carboni &amp; de Luca, 2016). Addressing these challenges necessitates interdisciplinary solutions. There is increasing recognition of the need for a comprehensive digitalisation theory that integrates semiotics with new media technologies (Nantke, 2017), aiming to enrich the digital representation of ICH (Berlanga-Fern&aacute;ndez, 2022). As Olteanu &amp; Ciula(2022) argue, &quot;digitalisation-when dealing with conversion across media-are forms of intermedial translation, hence of relevance to translation studies that found its theoretical grounding in semiotics&quot;. Building upon this, this research aims to contribute to bridging these gaps by developing a theoretical and practical framework based on multimodal translation theory. This framework, tailored for cross-contextual applications, will bridge traditional understandings with the digital realm. It will focus on digitally preserving and representing the intangible attributes, living nature, and multimodal characteristics of ICH. Aim and Objectives: The overarching research aim of this project is to craft a viable digitalisation pathway for the digital preservation and representation of multimodal ICH content that addresses both challenges and opportunities. The focus is on ensuring long-term development while respecting diverse and multivocal cultural contexts. The objectives are as follows: 1: Apply interdisciplinary multimodal analysis to decode the diverse characteristics of ICH, reflecting its multifaceted nature and significance in socio-cultural contexts. 2: Explore translation strategies for transferring ICH from traditional to digital formats, ensuring the preservation of its intrinsic meaning across different modalities and contexts. 3: Develop a flexible theoretically informed and practically-oriented framework for multimodal translation of ICH, enhancing its digital access and relevance in a rapidly evolving digital world. Contribution: This research introduces multimodal translation from an interdisciplinary viewpoint and aims to provide a theoretically-informed and practically-oriented framework for effectively preserving ICH in today's digital landscape. Academically, it forges a link between systemic functional linguistics and design theory, developing an innovative digital translation system that offers a richer and more culturally attuned representation of ICH. On the application level, the research enhances preservation and dissemination processes, employing advanced digital techniques for efficient data management and broader accessibility. This approach enriches ICH propagation, supports its systematic inheritance, and promotes cultural exchange through a shift towards more dynamic ICH engagement.2027-09-291291772D-DFCE-493A-AEE7-24F7EEAFE0E9AHRC2024-09-30INCOME_ACTUAL029308486CFA1E1F-F25C-4C23-8FE1-C47AE53E333EUnclassified0096EA78-5A08-49FA-9F1F-003344C4FA97Investigating the Regulation and Replication of R-loops at TelomeresActive2934080StudentshipEukaryotic chromosomes are capped at each end by specialised nucleoprotein structures called telomeres, which are mutated or altered in essentially all incidences of cancer. Telomeres are transcribed into a non-coding RNA called TERRA, which forms a three-stranded R-loop structure in which the RNA remains bound to the template. R-loops are particularly prevalent at telomeres in ALT-type cancer cells, where they are thought to prevent efficient replication of the chromosome end. This project will identify novel R-loop regulating factors at telomeres and examine how TERRA-based R-loops regulate and influence the telomere replication process.2028-10-05C008C651-F5B0-4859-A334-5F574AB6B57CMRC2024-10-06INCOME_ACTUAL029340806CFA1E1F-F25C-4C23-8FE1-C47AE53E333EUnclassified0525139E-02B2-4AEB-A8FF-00A621E82482Designing and exploring new quantum materials based on Fermi surface topological transitionsClosedEP/V000136/1Research GrantAbstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.2024-09-29798CB33D-C79E-4578-83F2-72606407192CEPSRC2021-03-31INCOME_ACTUAL404748Loughborough UniversityUnited KingdomHine (Warwick) and Betouras, Greenaway (Loughborough)E42EA860-69DC-479B-B62C-7A65F00768D3Work in progress as yet.6216430e621bb9.60893822-1Theory/Theory partnership, based around different approaches to theoretical modelling of materials.This new collaboration was the direct result of the project. My research group is able to contribute ab initio calculations which are highly useful to the Betouras group in developing models of Fermi Surface Topological transitions. We are still preparing our first joint paper on this but hope to have this submitted before the end of the grant.Academic/University2019-01-01By controlling the environment and interactions of layers in a 2D material, we can find and manipulate new quantum phases. Layers of two-dimensional materials stacked with a slight &quot;twist&quot; creates a long-range &quot;moir&eacute; pattern.&quot; The moir&eacute; pattern can systematically vary the geometrical and electronic properties of a material over long lengthscales. In the Warwick section of this grant, we have developed machine-learned interatomic potentials using the MACE package that are capable of representing interactions in a family of 2D transition metal dichalcogenides and post-transition-metal chalcogenides. This was realised in two key publications, Magorrian et al, Phys Rev Mater (2025) and Siddiqui and Hine, npj Computational Materials (2024).With the use of MLIPs for 2D materials becoming more widespread, there are many possible directions which could be pursued: one exciting prospect would be to build a &quot;Foundation Model&quot; for 2D materials, extending the capabilities of the successful MACE-MP0 models to be able to handle interlayer interactions in 2D.509031BC-4A6B-41E8-BEF8-26FC60BA51E965eb2ba5691af6.05958793Digital/Communication/Information Technologies (including Software)ElectronicsEnergyManufacturing including Industrial BiotechologyAs part of work in this project, we dramatically improved the capabilities of ONETEP in terms of simulated ARPES spectroscopy. The routines for Spectral Function Unfolding were re-written and accelerated many-fold (frequently over 10x faster). This has made them usable in new ways such as for taking constant energy maps across the whole Brillouin zone.3959BDAF-C1B1-4AC4-B308-51AFA5A5D375Further papers using SFU by other groups (eg O'Regan group)65eb2b7b124cb5.99533703trueImprovements to Spectral Function Unfolding Code in ONETEP LS-DFT packageImprovements to research infrastructurehttp://www.onetep.org2022Linear-scaling density-functional theory code for understanding and predicting the properties of materials from first-principles quantum mechanics.785FA424-B893-4289-B61C-67734568F6B5ONETEP is continually developed and new, updated versions are released on an annual basis. The developments associated with this grant were released during the period of the grant, between 2020 and 2025. It is one of the leading codes of its kind in the world and unique in being sold commercially: in 2004 it was adopted by Accelrys (now Dassault Systemes BIOVIA), a leading scientific software company, and has been one of the flagship products within the Materials Studio suite of software since 2008. An inexpensive academic license is also available worldwide direct from Cambridge Enterprise Ltd. Total revenue from ONETEP to date exceeds &pound;3M from over 200 organisations worldwide. The current projects have added extensive new functionality in the area of theoretical spectroscopy, leading to the ability to describe the angle resolved photoemission spectrum of systems such as 2D material heterostructures, and much more accurate treatments of spin-polarised systems.true67bc878641ea00.32512653ONETEP linear-scaling DFT codeSoftwarehttp://www.onetep.org2024607E0830-A9EE-4717-B4A2-FBE7DC3F8F0DStrain-dependent one-dimensional confinement channels in twisted bilayer 1 T ' - WTe 2Physical Review B91858b9cbcf9a6f0973ba2f256b89f6fMagorrian S2024-01-0166ae40928764f66FDAF29-0B6B-4C9C-AC06-4264166BDDB7Strong atomic reconstruction in twisted bilayers of highly flexible InSe: Machine-learned interatomic potential and continuum model approachesPhysical Review Materials91858b9cbcf9a6f0973ba2f256b89f6fMagorrian S2025-01-0167a2630356ae3EB2D2954-EAA5-4EDC-BFF9-F9D9BAD2B61FBand alignment and interlayer hybridisation in transition metal dichalcogenide/hexagonal boron nitride heterostructures2D Materials91858b9cbcf9a6f0973ba2f256b89f6fMagorrian S2022-01-016347f124587fc5B1B5120-D68E-4F8E-B1C0-244A66E7388AMachine-learned interatomic potentials for transition metal dichalcogenide Mo1-xWxS2-2ySe2y alloysnpj Computational Materialsde8bbbd2f23d85097d4f688b6e3fb00bSiddiqui A2024-01-0166d10074a0b724.51489037681C2F36-6C01-4A81-BD58-BB5AF3D8A80CBand alignment and interlayer hybridisation in transition metal dichalcogenide/hexagonal boron nitride heterostructures91858b9cbcf9a6f0973ba2f256b89f6fMagorrian S2022-01-0165e8c839c68c50.55722512EP/V000136/1177C04BB-BFD4-4D65-BA9D-15A50728B8CA30Materials sciences34214018-FC7E-4A53-8E6E-05A78B30749A70Supercond, magn. &quant.fluidsD3193E46-72CF-4206-8EF1-1752747471B270Condensed Matter PhysicsBEA752D6-11B4-4E5F-937C-2DD41104E22930Materials Characterisation054F6276-968F-45C5-AF49-0181DE4F2F6FResearch and Training to create assets in the form of new companies to develop a new model to generate innovative therapiesClosedMR/W004291/1Research GrantCancer Research UK has partnered with Deep Science Ventures to create an opportunity for early career stage researchers to build ventures that target the complexity of biology. This partnership brings together the world's largest independent funder of cancer research with the unique venture design process of Deep Science Ventures to create a programme that is exhaustive in the search for the best approach from both technical and commercial perspectives, and positioned for new venture growth from day one. Their aim is to identify and bring together entrepreneurial scientists, academic advisors and investors to design and build new companies in oncology. In this project, the research goal is to develop therapies by leveraging the wealth of data of this new era to identify ways to kill cancer cells so that can escape from treatments. My role will be to start as a Founder Analyst within DeepScienceVentures under their unique framework for venture creation. It's a part-time role working mainly involving research. In order to make this research successful, I will apply my skills sets of machine learning/artificial intelligence, understanding heterogeneity, developing patient-based biomarkers and hands-on experience in preclinical trials. This also provides opportunity to get trained in market analysis, and customer development together with the DSV team before incorporating our own start-up.Cancer is an evolution machine. Decades before diagnosis, a multitude of different clones, carrying differential sets of mutations are setting the scene that makes almost any targeted therapy obsolete. The new era of personalised medicine and of immense knowledge of the genetics, epigenetics, gene expression, metabolomics, and clonal evolution of tumours, converges with the completion of flagship synthetic lethality studies, interrogating pan-cancer functional genomics. However, target discovery still focuses on finding targeted therapies against well-established cancer drivers from which cancer has already evolved out of, even years before diagnosis. This results in limited response rates in the clinic and quick evolution of resistance for targets against such drivers for example BRCA targeted PARP inhibitors. Our Approach: We are stopping the chase for moving targets by building a highly advanced drug discovery machine, that leverages the wealth of data of this new era to identify vulnerable mechanisms that cancer cannot evolve out of and the specific context in which they are effective. To achieve that, we identify cancer systems biology and specific mechanisms of irreversible cancer dependencies through advanced computational methods. Our current approach sets the bar very high, to produce complete and sustained therapeutic responses. We have invented methods that have already produced potential candidates with strong independent validation.My role will be to start as a Founder Analyst within DeepScienceVentures under their unique framework for venture creation. It's a part-time role. As a secondee, my goal is to join the host institution to create a company that will bring novel curative therapies to cancer patients. In order to make this successful, I will apply my skills sets of machine learning/artificial intelligence, understanding heterogeneity, developing patient based biomarkers and hands-on experience in preclinical trials.2023-06-2960BD9E86-6D7E-4F7C-B408-E6774E43BA82UKRI Inn.Scholar2021-06-30INCOME_ACTUAL84549Francis Crick InstituteUnited KingdomKQ Labs - Accelerator Programme for early-stage startups4FBBC3C9-6C0B-444C-853A-EF6666E9CAA8NA622fc48bd66fc6.35301131-1KQ Labs is a five-month accelerator programme for exciting early-stage start-ups using data as a core part of their business model to improve human health, run by the Francis Crick Institute and funded by LifeArc.As a Secondee to Enedra Therapeutics, a Spin-off from Deep Science Ventures, I participate to learn to validate ideas and business models.Academic/University2021-01-01Cancer Research UKUnited KingdomCRUK Therapeutic Discovery LabBDEC01FF-DDE7-4AFD-A0E2-6DB9E941446BOngoing work.622fc53f49c518.03615788-1Through CRUK Therapeutic Drug Discovery Lab, we collaborate with them to validate our drug targets.As a Secondee to Enedra Therapeutics, a part of the Deep Science Venture, a part of my role is develop machine-learning models for drug target discovery.Charity/Non Profit2021-01-01NVDIA Accelerator ProgrammeParticipation in an activity, workshop or similarInternational11234DFE-039E-4168-8278-0D99B1E41320NVDIA provides opportunity to learn their AI tools and techniques67d2ec58a700c3.85194906falseProfessional Practitioners2025I developed a machine learning approach to identify biomarkers based on a drug target and another genomic aberration.C3751A61-F480-455E-81C3-9952F46DDD5DThis is expected to potentially personalise drugs, if our target gets developed into a drug.622fc686a72457.48231028falseMachine learning approach to identify biomakers for drug targetsComputer model/algorithm2021I developed a mixture linear model to identify drug targets from cell lines.7F14F4E0-FC63-4000-9750-3B7919A6D0B1This is leading to the identification of cancer-specific drug targets622fc5fe9197f6.07049081falseMixture model to identify drug targetsComputer model/algorithm2021MR/W004291/16CFA1E1F-F25C-4C23-8FE1-C47AE53E333EUnclassified059C2C14-4452-4CCB-BBE1-0080A54F0866CULTUral heritage in RurAL remote areas for creative tourism and sustainabilITYActive10107827EU-FundedThe main objective of the project is to contribute to the promotion of cultural and creative tourism activities, considering the different capacities, resources, and specificities (material, creative, human...) of the territories, as a mean to help with the sustainable development of peripheral rural areas, favoring job creation and population settlement. For this, the potential of its cultural heritage will be researched, taking into account both the artisan material culture (techniques, materials, patterns and decorative elements), as well as the intangible culture (music, oral knowledge and culinary traditions) as a resource. To promote non-seasonal tourism as a form of sustainable development with a stable population settlement we are going to research the crafts linked to the territory and their implications, but we will also carry out a documentation of the productive processes. First and foremost, we are going to cater to the necessities of the local communities and pay attention to aspects related to societal groups at risk of exclusion, such as women, the elderly and the youth. All this will be developed from a multidisciplinary perspective, for which we will count on the various teams —most of them linked to different countries— that will be part of the consortium. Each of these partners will contribute to the development, as well as to the final result of the project through input directly related to their area of expertise: for example, among the collaborators we have intellectual teams specialized in the digitalization of heritage, research, or communication and dissemination, as well as as various institutions that will act as a testing ground for the more practical parts of the proposal. However, if something will characterize our work, it will be the constant collaboration between all parties, as well as the exchange of advice and experiences that will help to enrich collective knowledge and guarantee optimal results.2028-03-30240CEBFD-1052-4EAC-88DF-D88A163D61C8Horizon Europe Guarantee2024-03-31INCOME_ACTUAL286866101078276CFA1E1F-F25C-4C23-8FE1-C47AE53E333EUnclassified08DC2362-B8F7-4E0B-B1FC-001FB7AF5B94ENGLISH HERITAGE TRUST CONSERVATION AND HERITAGE SCIENCE FACILITY - RANGER'S HOUSE, GREENWICH, LONDONClosedAH/V01241X/1Research GrantThe English Heritage Trust (EHT) cares for the National Heritage Collection of 400 historic sites and 3/4 million associated artefacts. The Facility The heritage science and conservation facility at Ranger's House acts as a central hub for core research and conservation activities carried out by EHT. The facility itself comprises five rooms in the apartment adjacent to the publicly accessible house (home to the Wernher Collection), the fine art conservation studio, and an outbuilding used as a store. The facility has grown organically and has reached a point where the scale of work outstrips the capacity of the facility - both due to space and the quality of equipment within. The Beneficiaries - People and Collections The English Heritage Conservation Science team has been leading the way in research on the environmental response of objects in historic environments. This work has been critical in allowing for the safe display and storage of the collection in historic buildings. Its novelty lies in the combination of close object/ building examination and analysis, with an epidemiological approach. We have been able to develop this novel approach because we look after similar objects that are exposed to a range of very different environments. The practical methods we have devised as a result of our research have enabled us to reduce the carbon footprint of conservation activities by 40%. The sector's interest in this work is demonstrated by an impressive 70 publications in competitive forums, the oversubscription to a series of 8 international courses covering management of showcases and an invitation to the 2018 Gordon conference on Scientific Methods in Cultural Heritage Research. We have also supported six PhDs - looking at response of lined canvas paintings, archaeological bone, archaeological glass and outdoor artillery - through the CPD scheme, SEAHA doctoral training scheme, AHRC Collaborative Doctoral Program and the Science and Heritage Programme. This research would not be possible without the Ranger's facility and its equipment, but it has been severely restrained by lack of space and outdated equipment - with constant failures or issues with software compatibility. The conservation studio at Ranger's House, often guided by scientific advice from the conservation science team, carries out practical treatments and technical analysis of the English Heritage collection of over 1500 easel paintings, many of international significance, and their associated frames. Technical examination advances our understanding of an artist's materials and methodology. Over the last five years our work has resulted in the reattribution of several paintings, notably to Botticelli and Titian, and much associated publicity for English Heritage has followed. Art historical findings relating to paintings by Rembrandt, Titian, Weenix and Beuckelaer have been presented at several national and international conferences and published in postprints and journals. To allow this treatment and research to continue, we need to update equipment in line with technological advances and remedy issues with the studio space, previously adapted from an old coach house The Project The amount of research needed, both within the EHT strategy and to support the sector, is not possible within the confines of the existing facility, nor with equipment more than 10 years old and suffering constant failures (and not supported by the manufacturers due to its age). We will redevelop the interiors of the facility and upgrade the store into a workshop in order to house equipment, and provide space for sample preparation and wood working for fine art conservation. We will also replace the most outdated pieces of equipment, to significantly improve the efficiency for research undertaken in the facility.2021-04-2947E8D760-6F07-4F7F-9D2B-1E1CDDEBBAA4Infrastructure Fund2020-12-08INCOME_ACTUAL622959Birkbeck, University of LondonUnited KingdomDepartment of Biological Sciencesanalytical collaboration with birkbeck collegeB28F60FF-67CD-422E-9B92-4E2A7C5F440BIdentification of wallpainting efflorescneces as predominantly calcite (TGA) was pivotal in conservation decisions. Quantification of amounts of akaganeite, iron, magnetite adn other corrosion products in observed degradation allowed interpretation of DVS curves measured in facility. The purity of the polymer boxes was determined by DSC along with glass transition temperature to aid in accelerated aging decisions. Interdisciplinary materials science adn conservation research.63ff4b60771ff0.37362576-1provision of lead coated PQCs and logger, thermogravimetric analysis, FTIR analysis, thermomagnetometry and differential scanning calorimetry.provision of showcases to test lead piezo electric quartz crystal (PQC) technology, provision of standard environments for same, provision of samples from Lullingstone wall painting deterioration and archaeological iron deterioration, provision of aged polypropylene and polyethylene storage boxesAcademic/University2022-01-01Polish Academy of SciencesPolandInstitute of Physicsinteraction with IKIFP on paintings030CC6C0-8284-43BC-8A0C-80EAEEEE1387none as yet653103f3a0cd01.59722271-1PhD student prepared the samples, travelled to Lab to run them over a four week period.Ran thirty paint samples on Dynamic Vapour Sorption, interpreted resultsAcademic/University2023-01-01Cranfield UniversityUnited Kingdomacoustic emission from wood borersEE87B0CA-0CFD-477A-81D8-35C29A5DA981none as yet65f432cc2caa09.83428178-1undertaking tests at Royal Botanic Gardens Kew, undertaking tests on HMS Victorytesting application of acoustic emission methodology to detect active wood boring insects in wooden timbers located HMS Victory, loan of acoustic emission equipment, training in equipment use, interpretation of signalsAcademic/University2024-01-01University College LondonUnited KingdomInstitute of ArchaeologyWork on PhD into glass deteriorationF29845A5-EC8B-43A4-BE6C-346182A2720Enone as yet63d80e7983bca6.60493723-1analysis of ancient glass samples from EHT collectionTraining and use of dynamic vapour sorption, use of aging chambers to condition silica gelAcademic/University2022-01-01National TrustUnited Kingdomanalysis of earliest cricket ball4FD6B6EC-DA4E-4433-9A29-FB90DA1F546Dnone yet679a40109ee472.68273534-1historical research on itemFTIR and Raman analysisCharity/Non Profit2025-01-01National TrustUnited Kingdominfra red scannign at National Trust Knowle paintings studioAF874909-52C1-40C8-A7BD-498D792E52F2The collaboration allowed us to build a good working relationship between EH and the relatively new paintings conservation provision at the NT studio.63e0c3b1906776.18648353-1none as yetUndertaking the IRR of the National Trust painting at the Knole House conservation studios enabled EH to establish the practicalities of off-site working with the new equipment in a safe space. The National Trust do not have access to IRR internally and the analysis of the painting, provided a clearer image of an indistinct area of text, potentially important for clarification of the sitter and date of the work.Charity/Non Profit2022-01-01Cardiff UniversityUnited KingdomEIS for PhDAF87F3FD-37E0-4B15-BE88-6DD4D1EBE948none yet679a407cb15b36.52711717-1aging of coatings on steelprovision of electrochemical impedance spectroscopyAcademic/University2025-01-01Imperial College LondonUnited KingdomPhD clear coatings for steel457AB7F9-24CD-4E65-A19D-397F3381509Bnone yet679a4464d8ba59.25462461-1access to Imperial tribology and engineering labs,supervision of PhD, provision of exposure sites, information on metal composition and degradation rate, environmental data, pollution data, previous coatings usedAcademic/University2024-01-01Victoria and Albert MuseumUnited Kingdomacoustic emission analysis of 7th century wooden pillar58D350BC-0EFE-426F-A52D-CE238CE438FCnon as yet, paper accepted for conference67c6b5dbe33560.13699182-1long term monitoring of the pillar, recording and collection of losses, environmental monitoring, building support to hold acoustic emission sensors in place, checking and downloading acoustic emission.deployed acoustic emission analysis kit to wooden pillar, undertaken DVS analysis of fragments from the pillarPublic2025-01-01Daresbury LaboratoryUnited KingdomXPS glassAA64A168-D7E8-4BDA-B805-495136FFCD17none yet679a43908fbdf4.95281840-1tunnelling XPS analysisproduction of historic glass samples, exposure at international sitesPrivate2024-01-01Birkbeck, University of LondonUnited KingdomDepartment of Biological Scienceswork on PhD in archaeological bone deterioration8614E0F7-928C-45D8-B73A-70FE0E3BEF4APhD awarded63d80d8aa67a13.59722698-1Analysis of bone samples from EHT collectionAccess and training in dynamic vapour sorption.Academic/University2021-01-01English Heritage Members eventA talk or presentationNational30E37FE3-1CC5-4F47-8D39-066B51721BD1Visit to Ranger's House for English Heritage members that included presentation of the technical analysis of paintings carried out in the paintings conservation studio during paintings conservation.651bf2ba31bc67.35121261falseSupporters2023materials testing seminarParticipation in an activity, workshop or similarInternational7439DAFC-9AC1-4A3A-B258-7B5F598C5134seminar discussing approaches to materials testing and pratcical demonstrations679a429a59c571.82554270falseProfessional Practitioners2024Kenwood volunteers visit to conservation studioParticipation in an open day or visit at my research institutionRegional19F28CAC-DF09-4672-BC26-CB29BD0DC6B5Tour of the paintings conservation studio for volunteers from Kenwood House, sparked questions and discussion on paintings conservation and Kenwood paintings.651bf0dabc5e89.44866310falsePublic/other audiences2023Diana Cecil by Cornelius JohnsonParticipation in an activity, workshop or similarInternational29F737F6-F361-4BD8-819C-F65FF3B590F7Completed technical analysis and conservation treatment, including use of Infrared Reflectography and microscopy to investigate painting technique and materials. National and international press coverage, as well as social media video https://www.instagram.com/p/C0BgzyOMQmQ/ and painting back on display 30th November 2023656f03ef866543.08315053falsePublic/other audienceshttps://www.english-heritage.org.uk/about-us/search-news/pr-fashion-for-fuller-lips-not-a-modern-phenomenon--english-heritage-reveals-jacobean-ladys-original-beauty/2023Tour for visiting professor from KU LeuvenParticipation in an open day or visit at my research institutionInternational30D42766-FA7E-4365-A60D-79D86098F679Visiting professor from KU Leuwen had tour of lab and studio facilities, with questiions and discussions on similar research and areas of work.67976fed155e83.41651871falseProfessional Practitioners2024Metropolitan Police Museum staff visitParticipation in an open day or visit at my research institutionRegionalACB0A61D-89F1-4004-8967-9B649F2DCB93Staff from the Metropolitan Police Museum visited the conservation studio and conservation science lab to find out about the work of the Collections Conservation Team and similarities to their own work.651bd0f5794b72.17135258falseProfessional Practitioners2023Reynolds research dayA formal working group, expert panel or dialogueNational26EDF21E-C53E-4932-AED1-5457654D74CATechnical findings of Reynolds paintings analysis including Infrared Reflectography (IRR) imaging and microscopy results discussed with other paintings conservators working on Reynolds paintings.651bf000c4a801.17188217falseProfessional Practitionershttps://www.youtube.com/watch?v=JscF6JMDC4Q2023Tour for donorsParticipation in an open day or visit at my research institutionRegional00DB9BB3-C4E1-4958-A80C-065F5E08FCB2Gave studio tour to donors, discussing analysis of donated paintings, informing attribution.6797982eb0b3f3.21794797falseSupporters2024marble hill house revived lecture seriesA broadcast e.g. TV/radio/film/podcast (other than news/press)NationalCF0EB753-F9DC-47D5-9703-B6FE2A4192F7lecture on YouTube on infra-red camera results on paintings at Marble Hill House63d8132dad64e4.53574325falsePublic/other audiences2022VIP tourParticipation in an open day or visit at my research institutionNational2953EA17-FFB3-41CE-8255-04A47899EDEBTour of the studio for VIP guest at Chair's reception.679798d4756002.76016049falseProfessional Practitioners2024Tour for donorsParticipation in an open day or visit at my research institutionNational55E614EE-FA60-406A-B19C-6E9C44473C09Tour of studio for donors to discuss analysis and treatment approaches.67979932c12542.77662700falseSupporters2024Tate conservation staff visitParticipation in an open day or visit at my research institutionNationalD9F69D6B-274D-463D-AC43-76552DE60BB5Conservation staff from Tate visited the conservation studio and conservation science lab to see the CapCo funded improvements and hear more about the team's work651bd1b0d0e299.16042448falseProfessional Practitioners2023workshop on use of oxygen depletion measurements for conservationParticipation in an activity, workshop or similarInternational143A38EA-6F26-41AD-9AEA-25E043AD337E26 participants took part in workshop to show use of oxygen depletion measurements. Over half have made follow up inquiries and purchased the equipment.63d0554736bcd1.99637070falseProfessional Practitioners2022articl ein EH members magazine about refurbishment of painting studioA magazine, newsletter or online publicationNational29D6F098-D750-468C-93EE-20A6BF506C5Barticle in English Heritage members magazine describing refurbishment of paintings studio and some of work undertaken63d80c547e0a12.20638219falsePublic/other audiences2022Historic Estate Conservation Committee visit to lab and studioParticipation in an open day or visit at my research institutionNational69544346-0AE6-4124-B39C-038041B552EEHistoric England's Historic Environment Conservation Committee toured the conservation studio and conservation science lab facilities to see the CapCo funded improvements and hear more about the Collections Conservation Team's work.651bd016b6a469.44008534falseProfessional Practitioners2023National Maritime Museum Conservation Staff visitParticipation in an open day or visit at my research institutionLocalC9B5AF98-E742-46A8-B5E2-167FB99E6AC6Conservation staff from National Maritime Museum visited the conservation studio and conservation science lab to see the CapCo funded improvements and hear more about the team's work and research.656861bd6840a1.72579208falseProfessional Practitioners2023Visit by Arts Council England, Government Indemnity Scheme staffParticipation in an open day or visit at my research institutionNational26C4B980-ABBA-40D2-BCFB-E92FE301A01FArts Council England (ACE), Government Indemnity Scheme (GIS) staff visited Ranger's House, including the lab and studio facilities. Discussion on how the equipment facilitated work and understanding for collections and feeds into environmental control and our annual reports for GIS / ACE.67976bd7f37611.26345322falsePolicymakers/politicians2024ICON staff and trustees visitParticipation in an open day or visit at my research institutionNational038967F5-8E4A-429A-9AF3-D9DBEFE8831DInstitute of Conservation (ICON) staff and trustees visited lab and studio facilities, with questions and discussion.65e1bbc1ef2df4.81312345falseProfessional Practitioners2024Reynolds exhibitionParticipation in an activity, workshop or similarInternational914C1118-A679-42E0-9F84-D75B1222CD7ATechnical analysis of four paintings and conservation treatment of two works including use of Infrared Reflectography and microscopy to investigate Reynolds painting techniques and materials. Information used as part of the exhibition &quot;Spotlight on Reynolds: Lord Iveagh's Favourite Artist at 300&quot;, at Kenwood House. Technical analysis included in Bloomberg Connects audio tour of the exhibition, and associated video https://www.youtube.com/watch?v=JscF6JMDC4Q. National and international press coverage.651c004596ba86.28814866falsePublic/other audienceshttps://www.english-heritage.org.uk/visit/places/kenwood/things-to-do/spotlight-on-reynolds2023Filming for Ormiston Academies Trust (OAT) careers pages OAT futuresA broadcast e.g. TV/radio/film/podcast (other than news/press)National4A1E1799-A1D5-4A22-9DFF-ABE993549DA2Conservation Scientist and Paintings Conservator filmed in the conservation science lab and conservation studio for careers videos. These will be put on the OAT futures website for the pupils at Ormiston Academies Trust to view. The trust has over 30,000 pupils in over 40 schools, 32 of which are secondary schools, spread across the country.651bd3cf808760.87832255falseSchoolshttps://oatfutures.co.uk/2023article in EH members magazine about new conservation science labA magazine, newsletter or online publicationNationalD5BC49B0-C2CA-4430-8D40-D2183A076392article in English Heritage Members magazine, circulation over 3000, several subsequent enquiries related to the article63d80b514e8476.66715520falsePublic/other audiences2022career taster session for local schoolParticipation in an open day or visit at my research institutionLocal75EE784A-98F0-48FA-ACE5-DF288B30AB348 pupils form Thomas Tallis school attended for visit focussing on STEM careers, which sparked questions and discussion afterwards63d810178acbc6.88219386falseSchools2022articl ein EH members magazine about refurbishment of painting studioA magazine, newsletter or online publicationNational25315940-DE8B-404A-A6C1-4A644D49D1A2article in English Heritage members magazine describing refurbishment of paintings studio and some of work undertaken63d80baa930a53.31021587falsePublic/other audiences2022Technical discussion of Bateman Master PaintingA formal working group, expert panel or dialogueNational0086629D-3824-494E-9523-C0A69B76B4CDInfrared reflectography images discussed and aided technical analysis and new attribution and working practices of the Bateman Master (contemporary of Canaletto) painting651bf205ec3af4.44657554falseProfessional Practitioners2023public talkA talk or presentationLocal391231B1-7CD6-46F9-BD4E-C34AF2D7AA15Public talk Conservation science at English heritage to Sevenoaks U3A science group, sparked questions and discussion afterwards. Two attendees volunteered at English Heritage sites afterwards.63d055f3b9e0d6.97192981falsePublic/other audiences2022visit by ICON internsParticipation in an open day or visit at my research institutionNationalD1A86CAC-B3C1-4388-8434-833049F8B8C0Interns from Institute of Conservation scheme visited the science lab and paintings studio63d80f70274563.89089357falseProfessional Practitioners2022Intern and student visit to Ranger's labParticipation in an open day or visit at my research institutionInternational97F31834-4190-4ADA-AC7A-B740E999B19CGave lab tour to Intern from Metropolitan Police Heritage Centre (who was completing postgraduate studies at University of Leicester and the internship formed part of studies) and student from City and Guilds of London Art School Conservation course. Gave awareness of conservation and especially conservation science and sparked interest in further study for undergraduate student.67976d8230f779.39042909falsePostgraduate students20243999950BelgiumEURAustriade_ATGoGreen2026-11-02European Commission H2020C2D3309E-6F10-48C0-BC93-9C885CF65E186230c4088ad338.99607916Public2022-11-011000000United KingdomGBPUnited Kingdomen_GBCreating a national integrated mobile and fixed lab for collections, historic buildings and sites2026-07-01Arts & Humanities Research Council (AHRC)AH/Z506242/1A94C2021-7AA0-4093-A0C4-26428F747A98679a3f084dc1d9.94042761Public2024-07-31The new facility has allowed more collaboration with researchers, with 6 major collaborations, despite COVID restrictions being in force for the early period. The stated priorities have all been advanced. The issues are large and will require significant research to initiate change within the sector. The research enabled has generated 20 peer reviewed papers, with significant additional material at publication stage and four papers in press. The award of the GoGreen project funding (the proposal was strengthened by our stronger analytical capabilities from CapCo) significantly enhanced our research ability in the areas covered by the project, sustainability and preventive conservation. The award of RIO and RiCHeS funding will dramatically increase our analytical and aging capabilities and capacity to undertake research. The main research areas from the CapCo application were; • Addressing the sector debate regarding RH fluctuations causing damage to objects, significant data produced on furniture response in real environments with acoustic emission; impact on egg tempera paintings (DVS); susceptibility of stone and ceramic objects (UV/vis/NIR, environmental chambers); requirements of miniature paintings on ivory (DVS, oxygen measurement for air exchange rates, environmental chambers); ivory response (DVS, universal tester, environmental chambers); damage mechanisms of glass and enamel objects (DVS, laser displacement measurement, environmental chambers). • Investigating mould growth and treatment: DVS analyses of both dust and cardboard storage boxes have allowed understanding of moisture- RH response which drives mould growth. • Preventing damage to archaeological materials: extensive work on archaeological iron and copper alloy (oxygen measurement, DVS), clays in stone (UV/Vis/NIR), stability of archaeological glass (DVS, UV/Vis/NIR). Environmental chambers and DVS used for archaeological bone and glass PhDs. • Testing and developing damage functions: Existing damage functions for copper and silver have been tested and compared to measurements of corrosion rate. New funding delivered (GoGreen project) to provide a post to undertake this work to generate new damage functions for glasses, iron alloys and stone. International exposures underway and materials sourced. • Treatments for outdoor objects: Internship of PhD student investigating paint treatments for twentieth century artillery in facility. PhD now awarded. • Exploring storage methods, which are key to survival of stored collections: Investigations into performance and long term stability of storage boxes for archaeological iron and copper alloy, bone and glass. Investigation into performance and long term stability of silica gels (Environmental chambers, DVS, tensile tester). New methods for much more stable storage developed and protocols for storing unstable glass investigated. The performance of portable pollution filtering devices has been investigated (UV/vis/NIR). A new grant, RIO will significantly extend our abilities to measure and determine the impact of pollution on collections and also RH fluctuations. Research has also facilitated the display of mixed archaeological objects in the new exhibitions at Richborough Roman Fort, Lindisfarne Museum and Wroxeter Roman Town. Able to support these projects with extensive testing of objects proposed for display including stability of archaeological iron objects and light stability of two prints (including the Girtin discussed below). . The re-opening of Marble Hill House was supported by the trials of air curtains to reduce air impact into the newly designed visitor route with the ultrasonic air velocity equipment. The APOLLO infra-red camera is being used as part of the paintings documentation process. Over the last year, 20 paintings have either been fully treated or prepared for loan out in the English Heritage paintings conservation studio and all are now back on display in properties across the country. A further 9 paintings will return to sites over 2025/26 once treatments are complete. The enhanced pantograph studio lighting system is in use every day, and each treatment utilised other equipment acquired through this funding stream. All paintings were examined with Infrared Reflectography using the Apollo camera, this work now forming a part of our documentation process alongside standard photography with the new camera equipment. Additionally many were examined with the microscope to answer questions relating to condition or technique, the new camera set up facilitating discussion amongst colleagues as well as enhanced documentation of findings.20224F85A3E7-F958-47EC-9347-D7F4F1C5B2B7Cultural6233261ff2ab77.80769525Culture, Heritage, Museums and CollectionsDevelopment of calculations to predict showcase conditioning efficiency relating to humidity control which has informed showcase design (environmental chambers, balance and DVS), presented in 3 publications. Combined with energy use measurements and life cycle assessment this has produced a model for the carbon footprint of showcases in heritage institutions, presented in 2 publications and 2 others under review. A method to determine the stability and measure the corrosion rate of archaeological iron and copper alloys has been developed (oxygen meters) and published (2 papers). This has allowed significant epidemiological research on English Heritages archaeological metals collection including discovery of a group of objects that deteriorate frighteningly rapidly at very low RH values. Developed a low cost method to estimate acetic acid in enclosures, replacing expensive instrumental analyses, that have been beyond the resources of many cultural heritage institutions and reported to be a significant limitation in surveys (UV/Vis/NIR, environmental chambers). One publication under peer review. Studies into the degradation of daguerreotypes of the Darwin family have allowed improvements for their safe display at Down House (environmental chambers), The lessons have been transferred to the field in a paper. The environmental chambers and DVS have been used extensively in two collaborative PhD projects. A new phenomena in the isotherms of archaeological glass has been discovered, that will have important impacts on its preventive conservation. The behaviour of furniture and wooden sculpture under fluctuating RH conditions has been studied with the acoustic emission equipment, indicating the safety of some environments and identifying others that need improving. The long term nature of such studies, to account for seasonal variations means publication is just beginning. One paper has been accepted but not yet appeared.Showcase humidity calculations already in use by 15 institutions (conservators, scientists, designers). Acetic acid adopted by 15 conservators and scientists. Oxygen depletion method used by 12 researchers, including in two major research projects and 20 others have been trained. For a small field such as conservation research, this is an exceptionally high take up rate. Results from daguerreotype glass deterioration is already informing other institutions displays and archaeological glass results will alter display and storage approaches.70A5D0A0-0FFA-4E53-9773-1EBCA3B6E7A06233249dec0783.48520029Culture Heritage Museums and Collectionsresults fed into drafting of CEN BS 15999 part 2 and rewriet of CEN BS 15999 part 1Europe2CCA0AB0-8308-41CB-BBAA-01028481A658The existing standard EN BS 15999 part 1 has been purchased over by over 3000 professionals (EN data) and is much more widely used by individuals with subscription services. Part 2 is due for publication in 2022 and deals with many technical aspects, which have generated a number of papers reporting failures in showcase performance. The two areas have been through robust review and been accepted by the technical committee and will significantly improve RH and pollution performance.6231bdc4124480.09149449Contribution to new or Improved professional practicemulti analytical method to determine stability or otherwise of historic glass objects11A7A3EA-D7DF-482D-9386-208E05637CC8Work using method at Rijksmuseum and Victoria and Albert Museum679a470495b3b7.56920345falseMethods to analyse glass to determine future degradation rateImprovements to research infrastructure2023Calculates carbon footprint and 8 other sustainability parameters for showcases. RiCHeS purchase of the EcoInvent LCA software and database has significantly improved the sustainability indices data.C1E00143-B393-4A9E-BF99-FE11BB37CA4Edownloaded 401 times, using in conservation training University of Catalonia679a4650d27082.39523643truecarbon footprint and LCA tool for showcasesImprovements to research infrastructurehttps://www.english-heritage.org.uk/learn/conservation/collections-advice-and-guidance/2022In 2021 the Fine Art Conservator implemented a simple method to measure the carbon footprint of an easel painting conservation treatment, including monitoring energy use, materials, object transport and staff travel. It uses widely available tools and resources, without requiring the help of a specialist sustainability expert. It represents a walk-through of the steps lay persons could take to replicate the study.81E9AA18-C468-44A2-8F61-B30F01A243A4Additional painting conservation treatments have been assessed to determine the carbon footprint. Information has been shared with wider ICON Sustainability Network and led to further research on sustainable packing materials for paintings.656f075035e707.70733678trueHow to Calculate the Carbon Footprint of a Paintings Conservation treatmentImprovements to research infrastructurehttps://www.english-heritage.org.uk/siteassets/home/learn/conservation/collections-advice--guidance/how-to-calcluate-the-carbon-footprint-of-a-painting-conservation-treatment_alice-tate-harte_david_thickett.pdf2023salt removal by poulticing, removes need to drill a sample from historic worked stone sculpture251EA92D-A7C2-4072-AD8C-010BA8C31534none as yet679a4778b01bd5.71545227falseMethods to analyse stone to determine future degradation rate and suitable preservation climateImprovements to research infrastructurehttps://www.english-heritage.org.uk/learn/conservation/collections-advice-and-guidance/2023carbon calculator for different methods of interventive and preventive conservation for silver collection32CB482D-C6F8-439F-82C8-39A28D2BF24Ddownloaded 67 times, used in conservation training Cardiff, University of Catalonia, CCI, UCLA679a459e3fa7a9.27170972trueperformance and sustainability impact of silver conservationImprovements to research infrastructurehttps://www.english-heritage.org.uk/learn/conservation/collections-advice-and-guidance/2022carbon calculator for different methods of interventive and preventive conservation for copper collections https://www.english-heritage.org.uk/learn/conservation/collections-advice-and-guidance/406B8347-DF07-4F92-8C0D-909D88D68E74none yet679a45d8b0f6f9.05627673trueperformance and sustainability impact of copper conservationImprovements to research infrastructurehttps://www.english-heritage.org.uk/learn/conservation/collections-advice-and-guidance/2024C64E98AB-CCDC-4CF2-9D4E-5F6A0D243F34Natural Light Aging of furniture, textile and bone in historic environmentsCultura e Scienza del Colore63e204f230453bc3cf81c8a84b87cfbcthickett D2024-01-01679a3cb3131787.845488570B4AE068-27C5-440C-B892-95FF3B25C352Calculating the Carbon Footprint of Interventive and Preventive Conservation at English Heritage, UKStudies in Conservationf209b1c00c8e7fb9be98e3dfc3c2cb59Tate-Harte A2024-01-01679a3d143fde88.9721428278F6A82C-4CD8-412D-8AC3-521622C32C7AInvestigating and Preventing Daguerreotype Glass Deterioration90e385ae1d92b951e8e0650069bd7798Thickett D2022-01-0163d053e4c3b271.450790503AB9E299-0A43-4A48-9580-D142785FE1B9Better Use of Showcases for Preservation and SustainabilityStudies in Conservation90e385ae1d92b951e8e0650069bd7798Thickett D2022-01-0163d050d851e4a9.7567540651711D59-74E7-41ED-BB88-230821043E13Analysis of Salts and Clays for Conservation of Porous Cultural HeritageApplied Sciences90e385ae1d92b951e8e0650069bd7798Thickett D2023-01-01657a0493369088.29747335B0D1DE53-AA61-498F-8318-2E8522A8725BOxygen Depletion Testing of MetalsHeritage90e385ae1d92b951e8e0650069bd7798Thickett D2021-01-016230bf594c1396.71844921193846D1-F156-410E-A679-E71C64F64D6AComparative assessment of paint systems for use on heritage artillery at coastal forts in England: experimental design and interim reportConservar Património89314afe407ab0f79cf15f9d0f030d85Smith W2023-01-01679a3a9ac62df6.42437647C4E5FD81-AD5A-4D85-AB6E-215B238C5C98Beyond Heritage Science: A ReviewHeritage5e86e30f1ab5028aa5e9597ef0d77c3bKennedy C2024-01-0165f431c29d7796.9072724644D7AF07-EFF6-487F-A77A-31E43E9D2AAEReview of analysis for cultural heritage conservationCurrent Topics in Analytical Chemistry436810896aa21c8f2b79f38009b1994ethickett d2020-01-016230c0cef2c9b4.96246046E0121022-19FD-4C1C-9504-8F877CA25E55Pollution and Heritage Metals-Effectiveness of Oddy Testing and Mitigation.Materials (Basel, Switzerland)90e385ae1d92b951e8e0650069bd7798Thickett D2023-01-011996-1944657a05037e59e9.789500809BD2778F-77E5-4C9C-AB3A-30965BC51755Measurement of Sorption Isotherms to Guide Mixed Display of Archaeological Iron, Bone, and Glass.Materials (Basel, Switzerland)90e385ae1d92b951e8e0650069bd7798Thickett D2024-01-011996-1944679a3d6f37ccb4.457430550A347DDD-3DC3-44B9-855B-0A589EBA1B24Surveying analytical techniques for a comprehensive analysis of airborne particulate samples in museum environmentsTrAC Trends in Analytical Chemistry78e9c1e710df451c16c7cd911964955dBrizzi S2024-01-016664523c6ae4b1.875684371C432ACB-619E-4110-962B-D531CEA5748FAnalysing objects to produce more sustainable conservation environments.90e385ae1d92b951e8e0650069bd7798Thickett D2023-01-01657a0767264974.99735048C3867FE2-6FAC-41D6-AA52-138BB824215EUsing Epidemiology to Validate Scientific Results for Complex Situations90e385ae1d92b951e8e0650069bd7798Thickett D2022-01-0163d052dfeb8b87.9856612914E9B07D-6C6D-43E8-A708-1CE5F8117270Practical Use of Damage Functions for Environmental Preventive Conservation and Sustainability-Examples from Naturally Ventilated BuildingsHeritage90e385ae1d92b951e8e0650069bd7798Thickett D2023-01-0163ff44e74ec3f2.6082549501210A47-3A79-4C30-B912-78902FF3BA97Managing Silver Tranish90e385ae1d92b951e8e0650069bd7798Thickett D2021-01-01653101a988a5c0.515579295C66B72D-0628-482D-A8B2-FAC500CC3953Assessing and predicting sustainability for maintainging silver collections63e204f230453bc3cf81c8a84b87cfbcthickett D2023-01-016530fd82a882e3.3268500869116CEA-1DA8-46F9-885E-CBDEB1F0E3E6Review of Interpreting Gaseous Pollution Data Regarding Heritage ObjectsHeritage90e385ae1d92b951e8e0650069bd7798Thickett D2023-01-0165e383da0c14c7.48754159DF4E8904-20F4-4041-927D-2249FD2F51F9Long-term provision of stable environments for metals conservation361d237589e2b1651cd5e7365063da4dLankester P2022-01-0163d05344dffe00.0274949609DBC308-BF68-4E76-A522-5A3320B75D50Analysing Objects to Tailor Environmental Preventive ConservationHeritage90e385ae1d92b951e8e0650069bd7798Thickett D2022-01-0163d05083f27ef1.97583128AH/V01241X/16CFA1E1F-F25C-4C23-8FE1-C47AE53E333EUnclassified095A3C0E-E4E6-4D44-945E-0036610164BANovel Time-Resolved Thermal Imaging: AlGaN/GaN Heterostructure Field Effect TransistorsClosedEP/D045304/1Research GrantThe increasing complexity of tasks required by communication, radar, aircraft, automotive systems benefits from the use of novel materials in high speed devices. Such devices, for example, radio-frequency (RF) transistors used in mobile communication base stations or phased array radars, have to meet certain performance standards. Electrical characterization is mostly used today to tackle challenges in the device development process to meet these standards. Electrical measurements, however, determine average device properties rather than specific information on spatial characteristics such as temperature and electric field inhomogeneities. If direct imaging of temperature and electric field distribution over a device area was possible with high time resolution this would open a new dimension for the investigation of semiconductor devices. This would be of great benefit to device researchers and developers to study and tackle time-dependent phenomena limiting device performance. Adequate techniques, however, are not existent at present. In the proposed work we will develop the first high-spatial resolution time-resolved thermal prober for semiconductor device imaging ever built to our knowledge. Electric field distribution will be extracted from the temperature information. The technique will be illustrated on the example of the topical AlGaN/GaN HFETs to learn more about how these devices operate in detail and what limiting factors for current devices are. For example, we will obtain information about carrier trapping related to AlGaN/GaN HFET current collapse, but experience shows that other interesting and potentially important discoveries are likely to result as well.2010-03-30798CB33D-C79E-4578-83F2-72606407192CEPSRC2006-06-30INCOME_ACTUAL417913Selex ESUnited KingdomSELEX Sensors and Airborne SystemsSELEX Sensors and Airborne Systems Ltd0109D8B7-F764-459D-8C0C-1B993A77B795b9c0ee8cb9c0eeaa-1Private2006-01-01QinetiqUnited KingdomQinetiQ (Malvern)QinetiQ (Malvern)ED8C406B-6B0E-44D4-84A1-E5AE2B641135b9c18c16b9c18c2a-1Private2006-01-01Renishaw PLCUnited KingdomRenishaw PlcACC544CA-BC6E-403A-85D6-9E2911010601b9c07fd8b9c07ff6-1Private2006-01-01The technique developed is presently used by us and new groups which subsequently implemented our developments to support defense and space industry product development, as well as on government funded (UK, EC, US) research programs.201059D2CB84-E584-40BB-BD82-2BDB941F5EC7Economic54466a091429f1.46471376Aerospace, Defence and Marine,Electronics,TransportA new technique was developed to probe temperature in semiconductor electronic devices with nanosecond time resolution, and submicron spatial resolution.Applicable for reliability testing of electronics in industry. Based on this project Bristol became partner in different programmes, such as NJTT led by TriQuint Semiconductors funded by DARPA. The technique is presently used in commercial setting to support industry. Based on the results software was developed that could be sold to interested customers in the future.6640FDAE-4F2B-420C-AAB5-BAD537FCDC34r-9033844833.2937857763a8d2ElectronicsCFCC521E-4C0D-40FE-BFA0-F48C932AF7D2Reducing Thermal Resistance of AlGaN/GaN Electronic Devices Using Novel Nucleation LayersIEEE Electron Device Letters0ff63e456f4cb53411fed2c6a845dfa7Riedel G2009-01-01doi_53d05a05afada7954D0ED775-E3F6-402D-BD99-A1DB9EFB8DC0Nanosecond Timescale Thermal Dynamics of AlGaN/GaN Electronic DevicesIEEE Electron Device Letters0ff63e456f4cb53411fed2c6a845dfa7Riedel G2008-01-01doi_53d05a05afb91387EBB4D70C-B680-4ACF-8DEF-8C9F49BD3FD3Channel Temperature Determination in High-Power AlGaN/GaN HFETs Using Electrical Methods and Raman SpectroscopyIEEE Transactions on Electron Devicesfb1ce42ed1ccadfa7cd5d45137f387a4Simms R2008-01-01doi_53d05d05d8e9cc180182EAA7-9742-4542-938B-ABBA621E6D00Nanosecond time-resolved Raman thermography: Probing device and channel temperature in pulsed GaN and GaAs HEMTs28ca9b64a2a9c24aa9c79896f256b0d2Martin Kuball (Author)2008-01-01m_437432809713efe396C837B93C-3CE6-4EC0-9525-7739198993AFBenchmarking of Thermal Boundary Resistance of GaN-SiC Interfaces for AlGaN/GaN HEMTs: US, European and Japanese Suppliers28ca9b64a2a9c24aa9c79896f256b0d2Martin Kuball (Author)2010-01-01m_730785260513efe2741F33B836-0CE4-45A7-85CC-F48960411554Time-Resolved Temperature Measurement of AlGaN/GaN Electronic Devices Using Micro-Raman SpectroscopyIEEE Electron Device Letters03a39e2937133e6c4136cf9a465bd7b2Kuball M2007-01-01doi_53d05a05af673956EE80B9D4-6ED8-45E7-BC9E-2828298EDAE4Current collapse in AlGaN/GaN transistors studied using time-resolved Raman thermographyApplied Physics Lettersfb1ce42ed1ccadfa7cd5d45137f387a4Simms R2008-01-01doi_53d034034d6d72faEP/D045304/11908FDF5-1C61-4F33-B47F-3E91675C88AA55Info. & commun. Technol.50CC55CC-BE0D-4167-BD99-285D6BCC369B20Materials processing945E0A55-10CB-4E91-BCCB-7CB22CFE223225Tools, technologies & methods4D4F63B1-6DA2-42B3-AAF1-0FC07D91E3D325Instrumentation Eng. & Dev.50CC55CC-BE0D-4167-BD99-285D6BCC369B20Materials processing8EC6A705-6D4A-44E8-95FF-27E262DA290C40RF & Microwave Technology811766AE-F6E0-4356-AEBF-17A4DA456AD415VLSI Design09E9D9A8-C863-48AA-B42B-004D4B4A472DOpen Access Block Award 2023 - Armagh Observatory and PlanetariumClosedEP/Y530037/1Research GrantAbstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.2024-03-311B0D1043-BA8E-4AF6-8022-B1BCC1E97FB9Open Access Block Grant2023-03-31INCOME_ACTUAL5693EP/Y530037/16CFA1E1F-F25C-4C23-8FE1-C47AE53E333EUnclassified0A9C4768-C996-4303-91F1-020F34284F78Characterising hydrothermal alteration across the Atlantis Massif: IODP Expedition 357ClosedNE/P000061/1Research GrantThe oceans covers approximately two thirds of the Earth's surface yet the oldest ocean floor is less than 200 million years old because it is continuously created and destroyed through the plate tectonic cycle. The ocean floor is made of volcanic rocks that form at mid ocean ridges, a global chain of under-water volcanoes that stretch for ~60,000km around the oceans, where two tectonic plates are moving away from each other. The rate at which the two tectonic plates move away from each other varies across the oceans. Currently 50% of the global mid ocean ridge system is spreading at slow spreading rates (&lt;40 mm/yr, e.g Mid Atlantic Ridge). From dredging and scientific drilling of the ocean crust and studying ophiolites, pieces of ocean crust that have been emplaced onto the continents, the overall structure of the ocean crust has determined. 'Typical' ocean crust has a layered stratigraphy with erupted lavas overlying intrusive feeder channels and frozen magma chambers (gabbros). However along slow spreading ridges this typical stratigraphy is not always present, and ~ 50% is formed by tectonic extension along detachment faults that bring gabbros and mantle rocks to the seafloor. Once new ocean crust is formed cold seawater penetrates downwards into the crust along fractures, becomes heated and reacts with the volcanic rocks until the hot hydrothermal fluids becomes buoyant and exit the crust at the seafloor . These reactions modify the chemistry of both the rocks by the formation of new hydrothermal minerals and the hydrothermal fluids, and are therefore an important process to quantify in order to understand global chemical exchange. The new minerals that form are strongly dependent on the initial rock and the temperature of the reacting hydrothermal fluids. At slow spreading ridges, the exposure of gabbroic and mantle rocks at the seafloor results in different chemical reactions, and mantle rocks in particular undergo extensive alteration to serpentinites. Serpentinisation reactions are accompanied by the formation of calcium carbonate minerals in fractures. The formation of calcium carbonate by fluid/rock reactions is currently being investigated as a potential long-term store of carbon dioxide. Understanding hydrothermal circulation in these environments is critical for understanding this process and ultimately exploiting it for the industrial storage of carbon dioxide. The Atlantis Massif is located on the Mid Atlantic Ridge and is an example of where tectonic extension has exposed gabbroic and mantle rocks at the seafloor. A hydrothermal vent system called the Lost City Hydrothermal Field is present on the southern end of the massif and is driven by serpentinisation reactions. Low temperature (&lt;100degC), high pH hydrothermal fluids vent diffusively at Lost City through carbonate-brucite structures. It is one of only five hydrothermal vents that are known to be hosted on mantle rocks. In this study, new samples recovered by scientific ocean drilling of the Atlantis Massif during IODP Expedition 357 will be used to investigate the role of hydrothermal circulation in the formation of ocean crust along these long-lived detachment faults. For the first time an age transect of samples across the massif has been recovered allowing insight into how the detachment changes and evolves as it progressively ages. By studying the new hydrothermal minerals that have formed during fluid/rock reaction, and documenting their distribution within the different rock types, the pathways for the hydrothermal fluids can be deciphered. This information will be combined with geochemical analyses of the rocks and hydrothermal minerals to quantify the chemical changes that have occurred during hydrothermal circulation across the Atlantis Massif. This combined approach will allow the contribution of hydrothermal circulation along detachment faults to the broader hydrothermal budget of global geochemical cycles to be determined.This project will make significant scientific advances towards our understanding of the role of hydrothermal circulation during the formation and evolution of the ocean crust. It will expand upon our current knowledge of this key Earth process through the variable slow spreading rate crust that represents much of the modern mid ocean ridge network. This project will primarily benefit the extensive ocean crust community, both those working on modern ocean crust and ophiolites, as outlined in the Academic Beneficiaries section. Benefits to: IODP The proposed research will contribute to addressing two of the proposed challenges in the 2013-2023 IODP Science Plan; challenge 9 'how are seafloor spreading and mantle melting linked to ocean crustal architecture' and challenge 10 'what are the mechanisms, magnitude, and history of chemical exchanges between the oceanic crust and seawater?'. This research will contribute to these by characterising and quantifying hydrothermal circulation through the detachment surface of the Atlantis Massif and its contribution to global geochemical cycles. Publication of this research in internationally recognised peer-reviewed journals will highlight the on-going importance of IODP as a world leader in scientific collaboration and high impact science. Benefits to: Public This research will contribute to topical global questions about the response of the Earth system to perturbations (global geochemical cycles) and the limits of life. Through the integration of this study with companion studies addressing the mechanisms of serpentinisation because of the intimate link between the two studies, this research will contribute to the topical debate about the long term storage options for atmospheric carbon dioxide. Involvement with University open days and public engagement activities (e.g. Girls into Geoscience) and the publication of the results in journals accessible to the public will ensure this research is exposed to the public. Benefits to: RA The research assistant will benefit from training and experience in laboratory procedures in addition to being involved with active research. It will provide an opportunity to extend their skill set and develop their future career prospects. Benefits to: Industry The long term storage of carbon in solid mineral form is one of the options available for reducing atmospheric carbon dioxide and is currently an area of research of high interest in both academic (e.g. IODP Expedition 357, ICDP Oman Drilling Project) and industrial (e.g CarbFIX) contexts. A necessary step towards the potential industrialisation of this process is understanding the natural system in a range of environments. This research is intimately linked to serpentinisation across the Atlantis Massif and will therefore help inform our understanding of the formation of calcium carbonate minerals in lower crustal and mantle rocks. This research will add to the growing body of research in this field and in the long term will be of use to carbon capture and storage industries. How does the proposed research generate impact? This research will inform our understanding of the variation in processes that form the ocean crust. The recent recognition of the extent of the detachment mode of seafloor spreading represents a major step in our understanding of how the Earth surface forms. The results of this study will provide crucial evidence for the interaction of hydrothermal fluids and tectonic processes and quantify for the first time the contribution of focused hydrothermal fluids on global hydrothermal budgets. The intimate link between hydrothermal circulation in the gabbroic and mantle rocks will inform our understanding of the natural storage of carbon in ocean crust, a crucial step in knowledge necessary for the potential industrialisation of this process.2017-05-178A03ED41-E67D-4F4A-B5DD-AAFB272B6471NERC2016-04-18INCOME_ACTUAL27616European Consortium for Ocean Research Drilling (ECORD)European Union (EU)Organising committee MagellanPlus workshop &quot;Investigating the oceanic life cycle of tectonic plates by mission specific drilling&quot;73E9CFBF-2C53-4436-9B5F-AB3840D4B568in progress.622b0aba22eda4.47465593-1I co-wrote the MagellanPlus workshop proposal and was nominated to be PI for this award (successfully funded at 15,000 euros). I am hosting and organising the workshop at the University of Plymouth UK, further cementing the leading role that UK scientists play in IODP.I am leading the organisation of this workshop, which builds on science themes but also the nature of IODP Exp 357 in the use of mission specific platforms.Charity/Non Profit2021-01-01Virtual Girls in Geoscience 2020Participation in an activity, workshop or similarNationalF805FB99-05D3-44C7-A01A-8BED8504203CI particppated as a panel member, with particular emphasis on sea-going fieldwork. My experiences from this Expedition (first with more than 50% female participants) featured in my answers603f80df6f2361.95744907falseSchools2020GiG 2019Participation in an activity, workshop or similarNational18CD17D8-3A52-4ABC-B332-BB0412DB9A13Results from Atlantis Massif featured as part of the Girls in Geoscience workshop &quot;Exploring the rocks beneath the seafloor&quot;5e54edbc7e92f2.92302306falseSchools2019Girls into GeoscienceParticipation in an activity, workshop or similarNationalB979F799-6EB6-43F4-99A1-4237BA459C7EThe event includes a mixture of presentations and workshops. This research was featured in a presentation highlighting the variety of opportunities available with an academic career in geoscience, along with highlighting the international projects that UK scientists are involved with.58c696b7e7b9d3.82413922falseSchools2016This research, along with participation in IODP Expedition 357, was featured in an event designed to encourage girls to pursue a career in geosciences (Girls into Geoscience, 3-4th July 2016, Plymouth University). This included a presentation to the registered participants (approx 100 students and teachers) highlighting my role in the expedition, the science being addressed and the opportunities available with international programs such as IODP. Feedback from the event highlighted that this contribution was very well received by the audience. Results and experiences of this research related to IODP Exp 357 was again featured in my participation as panel member at Virtual Girls in Geoscience 2020201608748C86-ECF2-4128-8E88-2DCECA642917Societal58c695283fde15.85060497EducationInitial results indicate a greater variability in alteration within a particular lithology across the detachment fault than previously expected. Geochemical analyses indicate a high variability in gabbroic alteration at a cm scale. These data will provide an example of alteration under a detachment fault and will be a useful comparison for gabbros altered around normal faults.Results will be presented at the postcruise meeting and I expect my results to be used by other members of the science party249EFEC9-70F6-4F80-A38F-6616EA91C66358c6935bb975e1.32723421Education79EDF258-5EFC-4D18-90E8-649CE089ACA8Magmatism, serpentinization and life: Insights through drilling the Atlantis Massif (IODP Expedition 357)Lithosb6a43c0732cdffa546b4fd4ba25dd5caFrüh-Green G2018-01-010024-49375c753067d95871.6557769899D7AFCD-995A-4C49-88EA-00D4F837D00CGeochemistry of serpentinized and multiphase altered Atlantis Massif peridotites (IODP Expedition 357): Petrogenesis and discrimination of melt-rock vs. fluid-rock processesChemical Geology6bad4a6264df2d091be74a6716dccc36Whattam S2022-01-01622b095cc2d802.97682558NE/P000061/1346611FD-47F5-46D6-9813-D4707F62253B100GeosciencesA646062E-6497-4533-8274-47644A7B369C20Earth Resources2334C846-D357-4EF1-B929-0EC4EC1854CB40HydrogeologyF52704F6-1035-4307-9FA9-45BC878F1F1A10Tectonic Processes7F1C4565-EFD3-4BE7-8A4A-B5F39FFD36F330Volcanic Processes0AE039A7-9A84-4943-AA36-001DB5763245Ferroelectrics for Nanoelectronics (FERN)ClosedEP/H023666/1Research GrantThe evolution of silicon technology since the 1960's has focussed on doubling performance and functionality every 18-24 months through miniaturization. Critical dimensions measured in tens of nanometres are now common place and billions of components connected by miles of wiring can be packed onto a wafer no larger than a thumb nail. Today the focus is shifting away from more scaling (called more Moore after the founder of Intel, Gordon Moore) towards increasing functionality through the introduction of mixed technologies on silicon (called more than Moore). This project investigates the incorporation of ultra thin ferroelectric materials into silicon nanoelectronics and two of its many applications.Capacitance is the rate of change of charge with voltage. It is the defining property of capacitors which are necessary in many electronic systems but are relatively large. Ferroelectrics can shrink capacitors by three orders of magnitude, because their electric permittivity is so high. More than that, their capacitance can be made to vary depending on the applied voltage so very small and tunable capacitors can be made, which can find applications in hand held electronics products in order to reduce power consumption. If they could be integrated onto a silicon microchip there would be further space savings. Thin layers are expected to produce even higher capacitance. However there is evidence that capacitance starts to reduce below 50 nm as dead layers are said to form near the interface with electrodes, but this may be an interface effect which can be lessened through engineering. Recently there has been experimental evidence that effective negative capacitance can be seen in ultra-thin ferroelectric films. If such material can be incorporated into a transistor then it would be able to reduce the voltage needed to switch a transistor between its on and off states (the sub-threshold slope). This would transform silicon technology, allowing a new generation of more powerful single core processors. Modern computers have dual or multi-core processors. A single core processor would generate too much heat but is still desirable for many applications. Capacitance places a lower limit on the sub-threshold slope. The consequence is that transistors need a larger applied voltage to be on and/or will leak current and so can never be fully switch off. This leads to increased power loss and heating as more transistors are crammed onto the same area of silicon, which limits component density. Integrating a ferroelectric film with negative capacitance into the gate of a transistor would reduce the overall capacitance and thus the sub-threshold swing. The need to understand and produce high quality ferroelectric ultra-thin films is imperative for each of these applications. Atomic Layer Deposition (ALD) at Newcastle and Pulsed Laser Deposition (PLD) at Imperial College will be used to deposit thin films of the ferroelectric materials barium titanate (BTO) and barium strontium titanate (BST). Both allow deposition thicknesses with atomic level precision. Extensive characterisation is needed to assess quality of these ferroelectric films. First principles computer simulation will be used to gain a better understanding of the films and to direct experiments. The deposition and thermal parameter space will be mapped to identify best ferroelectric properties for given constraints laid down by the silicon fabrication. Transistors will be made incorporating the best ferroelectric films to confirm the reduction in sub-threshold slope. Ferroelectric capacitors integrated onto silicon will be demonstrated, quantifying the capacitance increase per unit area and examining the fabrication constraints needed to maintain high transistor performance. This will also help identify integration issues, which also include equipment contamination and the development of ferroelectric etches.The RAs and PG student trained will have the opportunity to develop excellent analytical, research and communications skills. Such people have previously gone on to work as permanent academic staff, in industry, in finance and in government research labs. The project will offer other RA's and PG students an opportunity to benefit from working on closely related topics in the area of thin film ferroelectrics and it is anticipated that this will boost the activity to benefit all. UK companies spanning the supply chain for high performance integrated circuits will gain competitive advantage. The primary benefits will be proof of concept for new types of semiconductor devices using ferroelectric thin films and the reduction of risk for development and manufacture of products using these devices. Materials companies benefit from expertise within this consortium and IP generated. Knowledge gained will accelerate their progress in producing high quality films for many applications. They can license the recipes for deposition of ferroelectric thin films adding value to their deposition system. They will benefit from the collaboration, especially characterisation and device data which will reassure customers. Mixing silicon with ferroelectrics for high permittivity voltage controlled capacitors will have the benefit of allowing single chip solutions where previously several components may be necessary. Using ferroelectric films to reduce transistor sub-threshold slope is high risk but has the potential for enormous benefits. The exponential increase in microchip leakage power and heating, as critical dimensions reduce and transistor count increases has halted single core processor evolution in favour of multiple core processors in order to have effective thermal management. A reduction in power consumption by integrated circuits must be of global benefit to the environment, since almost every appliance uses some silicon technology. Beneficiaries will include not only the semiconductor manufacturers, circuit designers and product manufacturers, but all of us who use their products. Ferroelectrics are also piezoelectric and pyroelectric and so a range of intelligent sensor/actuator systems might be envisaged. While the UK does not at present have state of the art silicon manufacturing, it is likely that in future UK based companies will partner with overseas semiconductor foundries for the supply of part-processed wafers (the transistors and some interconnect metallisation) which can be completed integrating a variety of mixed technologies (such as thin film ferroelectrics for tunable capacitors) to create IP intensive products of high added value. This may be particularly appropriate for partnering within the EU where it can make economic sense to share expensive semiconductor foundries. Publication of research in high quality journals and at leading international conferences is crucial and will continue. The industrial steering group will be a means of two-way communication and engagement between this academic project and the commercial sector. National electronics networks like Si Futures, UKDF and EU networks like Sinano will be accessed. Press releases to the trade press will also be used to announce the project and to publicise breaking news as it develops. The research will feature on web pages of the two universities. The quarterly management meetings will have a standing item on potential impact of research. Promising strands of research will be pursued and our steering group members will be approached for additional guidance. Patents will be sought where possible prior to publication of the research. Both PIs have previous experience of knowledge transfer to industry. Both universities have excellent media staff to help with communication.2013-11-30798CB33D-C79E-4578-83F2-72606407192CEPSRC2010-05-31INCOME_ACTUAL528498Negative capacitance has been demonstrated experimentally. This has been used by other researchers and has encouraged further research as this is a demonstrated proof of concept. Other findings contribute to the body of knowledge relating to integration of new materials with silicon based technology7D5400A3-F8BB-425D-85BA-F502018D43EACultural5457674b20dfc4.71339318ElectronicsWe demonstrated experimentally negative capacitance using ferroelectric materialsour papers are commonly cited and there is worldwide interest to achieve steep sub-threshold MOSFETs using ferroelectric negative capacitanceE46F3A80-F16B-4F31-8160-8157619B1534r-5212102490.67479977641d6cElectronicsEnergy5D725BD5-C715-445A-A8D0-0A4D981AB0F4Anomalous resistive switching phenomenonJournal of Applied Physicsfeac839cf6a4aee7de53db71616eed78Mojarad S2012-01-01doi_53d038038b0397f1B94A941E-FE05-425A-9863-F9CD6CD6DFE4Effect of deposition conditions and post deposition anneal on reactively sputtered titanium nitride thin filmsThin Solid Filmscaac808b0061fcb1f2d914aacdba4defPonon N2015-01-01doi_55faa0aa040470ccF645BED7-62DD-49B8-BE35-84ADE1D682F9Experimental Observation of Negative Capacitance in Ferroelectrics at Room TemperatureNano Letters85813e14f3497b39b47b495e9282e3e2Appleby D2014-01-011530-69845457692b29fd98.95195290ED2DEDA2-206F-448B-86D5-330D9C970FFAFerroelectric properties in thin film barium titanate grown using pulsed laser depositionJournal of Applied Physics85813e14f3497b39b47b495e9282e3e2Appleby D2014-01-015457692b0124d8.38804738FDC8522A-3FE3-4716-89BD-D49D680FBD38Leakage current asymmetry and resistive switching behavior of SrTiO3Applied Physics Lettersfeac839cf6a4aee7de53db71616eed78Mojarad S2012-01-01m_54538498751367c95c769DB076-10B7-402F-92D5-3BEF484FB6C6Thermopower of LaFe 13-x Si x alloysEPL (Europhysics Letters)f09ad771b7c325be10100cb9f463cc0fHannemann U2012-01-01doi_55f95e95e8743dfdD90C995B-A447-4D0E-890F-E4D73EF4D4D2Oxygen vacancy migration in compressively strained SrTiO3Journal of Applied Physics23288bd9cd6a3d985580de295d68da23Al-Hamadany R2013-01-01doi_53d038038c27f938082F149E-1543-4A77-A8B6-A064FAE91649A comprehensive study on the leakage current mechanisms of Pt/SrTiO3/Pt capacitorJournal of Applied Physicsfeac839cf6a4aee7de53db71616eed78Mojarad S2012-01-01doi_53d03803802ab79dEP/H023666/11908FDF5-1C61-4F33-B47F-3E91675C88AA50Info. & commun. Technol.50CC55CC-BE0D-4167-BD99-285D6BCC369B25Materials processing177C04BB-BFD4-4D65-BA9D-15A50728B8CA25Materials sciences2B23EFD7-00EA-4FCA-8685-A8B4B94BF97650Electronic Devices & Subsys.BEA752D6-11B4-4E5F-937C-2DD41104E22925Materials Characterisation50CC55CC-BE0D-4167-BD99-285D6BCC369B25Materials processing0AF5D063-4580-42D4-B9A9-005CAB06FD35Literary Mapping: Dickens and the Dynamics of PlaceClosedAH/I02352X/1Training GrantAlong with a large collection of nineteenth-century maps of London and topographical materials, The Museum of London (MoL) holds a series of 41 watercolours painted 1860-1870 by J.L. Stewart of 'real places' in Dickens's novels. The Bishopsgate Institute holds a further 60 Victorian watercolours of Dickensian places. Other holdings at MoL include paintings, panoramas, and stereographic images; the covers and illustrations of the serial parts of Dickens's novels; later book-edition illustrations; the archives of Dickensian tourism at MoL (and at the Dickens House Museum), including plans of 'Dickens Walks' and Victorian 'Dickens' souvenirs. MoL also holds significant theatrical collections and large collections of photographs of London. To mark the bicentenary of Dickens's birth in 2012, a major exhibition will run at MoL from December 2011-June 2012, with an international tour to follow. The student would use the exhibition as a significant research resource for thinking about the continuing fascination with the geography of Dickens's London. S/he would be involved in creating a 'Dickens Walk' for the exhibition, also delivered through an phone app, and in running and presenting at events connected to it, as well as in detailed evaluation of its visitor response. \nApplications will be invited which address some or all of the following research questions:\nWas Dickens the only author whose work was mapped so closely to the 'real' during -and after - his lifetime? (comparators could be Scott, Hardy, Wordsworth) \nHow does the map of Dickens's places change over the nineteenth century?\nHow does an ever more pervasive print culture generate new ideas of 'place' and create specific 'places'?\nHow does the representation of place in 'fact-based' articles in Household Words and All the Year Round compare to Dickens's versions in the novels? (Significant places might include: Newgate, bridges, coaching inns, law courts, London churchyards, the City, and what Dickens called 'fairy land': the places of entertainment and theatre). \nHow did Dickens's own travels affect his construction of the provincial and the global? (Locations which might be considered include: the cities of Boston in the US; Paris; Rome and its ruins, Rochester, Preston, Broadstairs). \nHow did Dickens's texts themselves travel? How did his American and/or colonial readers in his lifetime interpret his sense of place? \nWhat was the impact of photography, and -later- film, on the poetics of place and particularly of London? Conversely, what was Dickens's influence on filmic 'placing' - in the early films of Griffith and Eisenstein, for example?\nWhat happens to place when it becomes distanced in time? How do we reconstruct Dickens's places today? \nWhat have been the effects of the heritage industry and mass culture on Dickens's work?\nThere has been much debate about 'place' as historically contingent process in recent years. The thesis will engage with work generated by scholars of geography, history, literature and art, such as David Harvey; Nigel Thrift, Derek Gregory; Henri Lefebvre; Raymond Williams, Lynda Nead and Franco Moretti.\nThe student would have a hot desk space at MoL and access to all the graduate-student facilities at KCL. S/he would spend at least one day every week at MoL, and often more, particularly during the first year, in the run-up to and during the 2012 Dickens exhibition which will stimulate and feedback into the initial research. In years two and three the student will add value to the documentation around MoL's Dickens-related collections, in the light of his/her doctoral research, and will choose and curate a group of objects for an on-line exhibition in year 3 in line with the MoL's ongoing research strategy. Academic supervisions would be supplemented by meetings at least every two months between Werner, Pettitt and the stuDickens is exceptional as a literary figure who continues to excite immense international interest and his bicentenary in 2012 will generate considerable media attention. Both Werner and Pettitt are experienced in giving media interviews and dealing with the press (Pettitt was interviewed on Radio 4's Today Programme, among others, about her last book; and Werner regularly speaks to the press) and are well-placed to capitalise on these opportunities, and maximise the media exposure of both the exhibition and the connected research at MoL.\n\nPettitt is planning a new undergraduate course on 'Dickens and London' inspired by this project. The beneficiaries of the collaboration will include but will also extend well beyond students (UG/PG) and academic staff at KCL and Museum staff at MoL. They will include a wide variety of museum/exhibition visitors, both 'real' and on-line, such as school children, adult learners, academics and journalists, and we expect international impact, both through tourists attending the exhibition and its supporting events, and, in the longer term, through hits on the legacy 'Dickens exhibit' website. \n\nThe student will also be involved in organising and presenting at a series of exhibition-related events, some at the MoL and some at KCL which will be free and open to the public. KCL has a new partnership with Cheltenham Literary Festival so a Dickens event could appear on the 2012 Festival programme too.\n \n'Literary Mapping: Dickens and the Dynamics of Place' will provide new insights into London's past, helping to inform the public histories presented by the Museum in its displays, online and through other outputs. The Museum recently created one of the most successful phone apps interpreting London's historic topography (Streetmuseum), and the student will be well placed to build on this and help the Museum deliver another innovative resource that will reveal the London of Charles Dickens to the general public. Considering the worldwide interest in the works of Dickens, such a resource is likely to have a broad appeal both to national and international visitors as well as those who live and work in the capital, and the intention is to deliver it in a range of languages. It will impact on the Museum's marketing campaign in drawing people to the exhibition (the target has been set at 100,000 visitors between December 2011 and June 2012). It will have a financial impact by increasing revenue for the Museum and other related attractions and sites across London.\n\nThe student's work will demonstrate the potential of the Museum's collections as a research resource and add value to the Museum's existing body of knowledge about its collections. The delivery of an online exhibition around the Museum's Dickens-related collections will create a further publicly accessible output and contribute to the Museum's reputation as a national and international resource on London and, in this case, specifically about literary London in the nineteenth century. It will also form part of the MoL's ongoing commitment to defining itself as a rich research resource both for scholars and for the public.\n\nCrucially, though, the impact of this project will not be only 'one way' - pushing outwards from the MoL towards the public. It will also close the loop and feed back the responses and ideas of the public into MoL's on-line displays. The student will have the opportunity to do this both through devising creative ways of eliciting reactions and responses at the exhibition from different constituencies of visitors, and through the detailed evaluation of this visitor response, and its use in considering the meanings of 'Dickens' to different interest-groups.\n\nThis innovative collaboration between curatorial and academic specialists has the potential to deliver both a highly stimulating doctoral apprenticeship, and2014-09-291291772D-DFCE-493A-AEE7-24F7EEAFE0E9AHRC2011-09-30INCOME_ACTUAL60250AH/I02352X/16CFA1E1F-F25C-4C23-8FE1-C47AE53E333EUnclassified0B005E1C-FEEE-4784-A0B9-01A0D57AE63BRegulation of protein synthesis as a mechanism of nutritional programming and developmental origins of optimal healthClosedBB/F014279/2Research GrantThe diet of an individual can have important health consequences at any stage of life. However there is evidence to suggest that the diet of women during pregnancy and while breastfeeding is particularly important as it has major long-term implications for the health of her baby. It has been shown that individuals with a low birth weight are not only less likely to survive delivery but are also at substantially increased risk of developing type 2 diabetes, high blood pressure and heart disease in adulthood and are more likely to die at a younger age. The underlying causes of the relationship between early growth and adult disease are not known, but it is thought that the quality of the diet of women during pregnancy and breastfeeding is critically important. Studies in animal models can be very useful in helping to decipher mechanisms of human diseases. We have shown that if pregnant and lactating rats have too little protein in their diet their offspring are small at birth and later develop diabetes. We have studied fat tissue and have identified key proteins that are much less abundant both in young men who had a low birth weight and in offspring of protein restricted rat mothers. The central dogma of biology is that the genetic material DNA is copied to an intermediary molecule RNA that is then translated into proteins. Our recent results suggest that the second step of this process is permanently altered (or 'programmed') in the offspring of animals or humans that had a sub-optimal diet. In our project we will study RNA from rat and human fat cells to identify new genes that are programmed to be expressed at different levels as a result of poor early life nutrition. We will also determine why it is that their RNA is not translated properly to make the corresponding proteins. Understanding these processes will enable us to identify individuals at risk and to develop intervention strategies to improve the health of pregnant and breast-feeding women and their offspring.Fetal and early life nutrition has long-term (ie programmed) consequences for health. This has been termed the Developmental Origins of Health and Disease. Despite numerous studies confirming the concept of nutritional programming and its associations with long term health, the molecular mechanisms by which a phenomenon that occurs in utero or during very early life has phenotypic consequences many years later are poorly understood. This proposal addresses the fundamental underlying molecular mechanisms of nutritional programming. We hypothesise that programmed responses to sub-optimal nutrition in early life are significantly mediated by regulation of gene expression at a post-transcriptional level. We further hypothesise that diet-induced changes in regulatory factors such as miRNAs alter cellular memory and consequently impact on long term health of the organism. We will adopt an integrated approach to test these hypotheses, combining whole body nutritional programming, functional genomics and transcriptomics using both the well established maternal low protein rat model and human biopsy material. This will include both genome-wide and gene-specific analysis of adipose tissue. Understanding the mechanisms involved in nutritional programming could ultimately lead to diagnosis, prevention and treatment of the long term effects of diet on health.2011-07-30198E4A3D-B2DC-45D4-8351-7CCEC4061876BBSRC2010-06-30INCOME_ACTUAL109803Seminar Sanquin ResearchA talk or presentationInternationalD0B433D4-75C3-44A5-8617-1E6321E66A98100 researchers attended talk. Seminar and Masterclass, Sanquin Research &amp; Landsteiner Lab., Amsterdam new collaborationsKePtZtGV8S8falsePaper PresentationOther academic audiences (collaborators, peers etc.)2012,2013Seminar Human Genetics Unit EdinburghA talk or presentationNational8075AA58-DA66-407C-96AD-9454C1627E0DMRC Human Genetics Unit, Edinburgh Interest in work from colleaguesCmtziWyEppUfalseKeynote/Invited SpeakerOther academic audiences (collaborators, peers etc.)2013Seminar University of Tor Vergata RomeA talk or presentationInternational4A8D50F7-EA61-4E47-89EB-207E48C0FB62Pharmacy Summer School, University of Tor Vergata, Rome Attended by students who showed interest in the research areaf3SkbLQkJq7falseKeynote/Invited SpeakerOther academic audiences (collaborators, peers etc.)2013Seminar University of SurreyA talk or presentationNationalF0A50658-F99E-4D93-9ED3-B442AEDDF7E6May 23 - Dept. of Microbiological &amp; Cellular Sciences, University of Surrey new collaborationsR7J2qsiDuextrueKeynote/Invited SpeakerOther academic audiences (collaborators, peers etc.)2013Unit Open dayParticipation in an open day or visit at my research institutionNationalF8392FEB-E8F3-48D7-868F-859298960379Over 500 members of the pubic visited the Unit Member of the public were very appreciative. Students asked to come and carry out work experience in the UnitAZ2qZmBoiJxtrueKeynote/Invited SpeakerPublic/other audiences2013conference RoscoffA talk or presentationInternational854EC169-5F9E-4F4A-B536-1765BD30530ATalk to colleagues, new collaborations establishedtYGqZG9uhDmfalsePaper PresentationOther academic audiences (collaborators, peers etc.)2012Seminar Queens University BelfastA talk or presentationNational2B489F3A-2883-4FDB-946F-384E019534D3September 24 - Centre for Cancer Research &amp; Cell Biology, Queens University, Belfast. Interest in research areaci1dzfcWt5BfalseKeynote/Invited SpeakerOther academic audiences (collaborators, peers etc.)2013180000United KingdomGBPUnited Kingdomen_GBproject grant (Co applicant with Cambridge)2015-10-01Diabetes UK2BFDA201-AE75-4BA6-BC13-D6ACBACBB3FApFCGiD6cpnSCharity/Non Profit2012-08-31We have shown how the offspring of animals exposed to a low protein diet have genetic changes which will increase their likelyhood of developing diabetes in later life.New areas of research11FCF0DD-C7CD-44FA-B79B-861B1141566D56dea099c06401.28725927HealthcarePharmaceuticals and Medical Biotechnology6FE4C637-69F3-461F-86AE-08C18F5F6364The involvement of microRNAs in Type&nbsp;2 diabetes.Biochemical Society transactions5b9440a2c5455ceea23242c0ebf37f89Ferland-McCollough D2010-01-010300-5127bSdVBA443zE7E30A321-1137-4D78-9A22-8C714D45088CProgramming of adipose tissue miR-483-3p and GDF-3 expression by maternal diet in type 2 diabetes.Cell death and differentiation5b9440a2c5455ceea23242c0ebf37f89Ferland-McCollough D2012-01-011350-9047pm_15229_29_22223106931D2B14-F77C-4CDB-B432-2FB04ED4B79FRNA binding protein/RNA element interactions and the control of translation.Current protein & peptide science9713132b92dcd31dc9edc16fb86ed6dePichon X2012-01-011389-2037pm_15229_29_22708490C2191451-EA87-4831-A07D-73DCA65E3BCARemodelling of a polypyrimidine tract-binding protein complex during apoptosis activates cellular IRESs.Cell death and differentiation10716f916a92f1e277d57baad1c31dceKing HA2014-01-011350-9047pm_15229_29_24141718BB/F014279/2DA27012D-4394-4161-A183-9FECAD961038BB/F014279/1304931.132008-08-032010-07-020B005E1C-FEEE-4784-A0B9-01A0D57AE63BBB/F014279/2109803.842010-06-302011-07-30999F0B31-F127-410A-A520-963B336BECE712Cell biology1D119F53-9239-4168-85B9-6DE4EE0B48D051Food science & nutrition88E467D8-9675-4A34-981C-F517896062B425Genetics & development29F3DF16-3094-4F79-BC69-8D05FB55182612Omic sciences & technologies2A0F6391-E88A-4396-9D63-25A68EEDA63512Communication & signalling3ADE3DAF-6018-4A3F-98C6-BDBB393095F451Diet & health23DCA711-FFA6-4DCF-A65B-9A8B653571EC25Gene action & regulation937A9F23-021A-4604-8979-A28E0E04F82512Transcriptomics0B36FFF3-3789-4DD3-92DF-0184F051FD2FISOL-SRS: ISOL Beam Storage Ring SpectrometerClosedST/M00161X/1Research GrantAbstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.Knowledge transfer: We foresee a number of areas where knowledge transfer could occur. All detectors used with the external solenoid must operate successfully in high magnetic field. In particular, we have highly pixellated silicon detectors. The challenges making these highly-integrated detectors function in this environment are extremely close to the demands of combined PET/MRI or SPECT/MRI, seen as a high priority for medical imaging in the future. Highly integrated electronics and ASICs may also be transferred over to the medical or other relevant sectors. The detectors to be used inside the storage ring must survive baking out to high temperatures and be compatible with very high vacuum. This may have applications to other areas of science. In the exploitation phase, the ISOL-SRS system could be used to gather nuclear data relevant to fission reactors, decommissioning or future fusion reactors. A specific example is the ability to measure beta-delayed neutron branches of ions stored in the ring. All the groups have a strong background in knowledge transfer. The Liverpool and Daresbury groups have experience in transferring nuclear physics detector technology towards the medical sector in areas such as SPECT imaging. York established an industry-facing detector development laboratory working on coupling scintillators to novel photosensors. Manchester has the Dalton Institute in-house who provide a natural linkage to the nuclear energy sector, while Surrey have a diverse group working in applications. Manchester, York and Surrey working on nuclear data relevant to present and future fission reactors, supported by an EPSRC grant. Knowledge transfer may be facilitated between groups and companies concerned with applications in nuclear measurement techniques and instrumentation, including GE Healthcare, BAE Systems, AWE, Canberra, Centronic, Kromek, Canberra Harwell UK, Ametek (Ortec), John Caunt Scientific, National Nuclear Laboratory (NNL) and Rapiscan. Liverpool has a 4 year STFC IPS Fellowship to maximize the impact of the STFC science portfolio. The Fellow will work closely with Liverpool, STFC Daresbury Laboratory and the Cockcroft Institute for Accelerator Science. The role will deliver increased numbers of industrial studentships, enable &quot;pump priming&quot; of collaborative ideas through appropriate routes such as mini-IPS or mini-KTP projects and will facilitate potential staff exchanges with industrial collaborators. The Edinburgh Nuclear Physics Group has been at the forefront of nuclear physics applications of silicon strip detectors since this technology became commercially available. They have a long-standing relationship with the UK company Micron Semiconductor Ltd (MSL). With MSL, they have developed silicon strip detector designs with very thin (~20 micron) and thick wafers (~mm) for our research applications. MSL now has a range and depth of technical capability unmatched by any commercial company worldwide and this technology is now applied to X-ray and space-based applications. Public engagement: There is considerable scope to engage with a variety of general audiences from schoolchildren to the wider public. Big science like nuclear/particle physics and astronomy is acknowledged as one of the key motivators for young people to decide to study Physics at university level and for a career. This project has an inspirational story attached relating to our fundamental understanding of nuclear structure and our understanding of the origin of the chemical elements. We are developing new instrumentation for the world's most famous scientific laboratory. The test bed solenoid magnet has featured in an article in New Scientist. Ongoing educational initiatives include Nuclear Physics Masterclasses for schoolchildren held at Surrey, Liverpool and York, supported by outreach officers co-funded by the Ogden Institute. Public engagement work will be facilitated by the STFC outreach officer, Elizabeth Cunningham.2020-03-30D7F4F462-0518-4784-908A-D12633C139B3STFC2015-01-01INCOME_ACTUAL913023Binding Blocks Winter Masterclass WebinarA talk or presentationNationalBBAE51DE-9B4C-4257-9787-9F7A70C1AA6FA webinar about experimental nuclear physics and studying exotic isotopes at the winter masterclass with the Binding Blocks team64072d2fa23c70.50704107falseSchoolshttps://sites.google.com/york.ac.uk/bindingblocks/post-16/webinars-winter-2022232022I'm a Scientist, Get Me Out Of Here (CERN Zone)Engagement focused website, blog or social media channelNationalBDE32551-8347-4095-82DF-1A11A0CD6DA3I am leader of the ISOLDE Team in the CERN Zone of I'm A Scientist Get Me Out Of Here, which directly engages Y10-13 students in online chats, Q&amp;A forums, laboratory updates/tours, etc.64071d74b9bb15.32578062falseSchoolshttps://cern22.imascientist.org.uk/team/isolde2022,2023Binding Blocks Summer Masterclass WebinarA talk or presentationNational13974629-7300-4142-B318-257DFBD8B21BA webinar about experimental nuclear physics and studying exotic isotopes at the summer masterclass with the Binding Blocks team64072ce65a5d38.54397517falseSchoolshttps://sites.google.com/york.ac.uk/bindingblocks/pre-16/2022This award was to design and realise two state of the art detector systems for nuclear structure and nuclear astrophysics studies. These spectrometers will investigate nuclei at the extremes of stability created following stellar events such as super novae. A key unknown for understanding the origin of the elements are the reactions and properties of unstable nuclei. Such nuclei are present in hot, dense explosive stellar environments and strongly influence the path of nucleosynthesis, and energy generation. It is becoming clear that the properties of these unstable nuclei cannot be easily extrapolated from our knowledge of stable nuclei, in particular the development of surprising new shell structures driven by the nature of the underlying nuclear interaction. In order to address such science in the laboratory, measurements of nuclear reactions and properties of unstable nuclei must be studied with a precision sensitive to their underlying quantum structure. Additionally, access to a wide range of radioactive beam species is critical for the scientific reach of the programme. This project involves the use of the established ISOLDE facility at CERN, with the widest range of intense radioactive beams (&gt;700 isotopes). Two spectrometers have been developed, one for use within a heavy ion storage ring and the other an external spectrometer coupled to a superconducting solenoid. During the course of the grant, the storage ring planned for ISOLDE CERN from TSR Heidelberg was cancelled and it was decided to locate the second spectrometer in the CRYRING storage ring at the new radioactive beam facility, FAIR in Germany. The two spectrometer systems, combined with the vast range of isotopes produced at ISOLDE and the cooled beams at CRYRING, will address a wide range of topics in nuclear astrophysics and structure involving unstable isotopes. The in-ring spectrometer will perform high resolution, high luminosity, in-ring direct and indirect reaction measurements with light and medium mass nuclei relevant for understanding explosive nuclear astrophysical processes and perform radiative capture reaction measurements also relevant for these processes. The external spectrometer (ISS) is located in a solenoidal system that will be used for precision studies of inelastic scattering and transfer reactions. This spectrometer has an advanced design that employs the proven HELIOS concept from Argonne National Laboratory. It will exploit beams directly from HIE-ISOLDE and will benefit from special beam manipulations. Both detector systems comprise Si detectors in a compact design, digital electronics, software for data acquisition and control, and mechanical chambers and supports. The project is well embedded in both CERN-ISOLDE and FAIR-CRYRING with strengthened relationships in both places. At FAIR the in-ring spectrometer has full acceptance into facility and its value will contribute to the UK's in-kind contribution to FAIR. At CERN the ISS spectrometer is regarded as a high profile addition to the HIE-ISOLDE facility. Apprentices, students and post-doctoral research assistants as well as the academic staff and experience engineers have benefited from this project in working and developing advanced instrumentation.The spectrometers are part of the research infrastructure at both CERN-ISOLDE and CRYRING FAIR. They are primarily for academic scientific investigation of exotic nuclei key to our understanding of the creation of the elements. As leaders of the two spectrometer projects the UK scientists will perform key experiments to address outstanding questions in this field. However, the instruments will be used and the data from them, by the international community of nuclear physicists, both experimental and theoretical, who will benefit directly from this new knowledge and understanding. The technical developments needed to make these projects a success include advances in radiation detectors, simulations, digital electronics high vacuum systems, cryogenics and data acquisition systems that are of benefit to all areas that require efficient and spectroscopic detector systems. These areas include medical imaging, homeland security, nuclear decommissioning and environmental monitoring. The collaboration has sought to publicise progress during the project. This has resulted in articles for the general public appearing on the web sites of STFC and CERN, as well as in the publications Fascination and CERN Courier. The results of an early implementation experiment were published in Physical Review Letters(https://doi.org/10.1103/PhysRevLett.124.062502). Other coverage: https://stfc.ukri.org/news-events-and-publications/publications/uk-news-from-cern/uknfc78/#two https://stfc.ukri.org/news/uk-apprentices-assist-in-recycling-a-hospital-mri-scanner-to-study-the-stuff-of-stars-at-cern/ https://home.cern/news/news/physics/isolde-steps-unexplored-region-nuclear-chart https://stfc.ukri.org/news-events-and-publications/whats-happening/daresbury-technology-helps-beam-physicists-into-unexplored-territory-at-cern/ https://acceleratingnews.web.cern.ch/article/isoldes-new-solenoid-spectrometer https://www.anl.gov/article/argonne-and-cern-weigh-in-on-the-origin-of-heavy-elements https://phys.org/news/2020-02-isolde-unexplored-region-nuclear-exotic.html https://www.eurekalert.org/pub_releases/2020-03/dnl-aac033020.php https://www.liverpool.ac.uk/physics/research/nuclear-physics/projects/isol/ https://stfc.ukri.org/research/nuclear-physics/isol-srs/ http://npg.dl.ac.uk/isol-srs/index.html https://isolde.cern/experiments/isolde-solenoidal-spectrometer-iss https://stfc.ukri.org/news-events-and-publications/whats-happening/carme-sets-off-from-daresbury-to-germany/780863BF-5938-4FED-870F-9859C48BB0F056d70e2acfbd21.18771367Digital/Communication/Information Technologies (including Software)EducationElectronicsEnergySecurity and Diplomacyhttp://npg.dl.ac.uk/isol-srs/index.htmlA code to read and process the raw data from the ISOLDE Solenoidal Spectrometer experiment at CERN. It builds physics events and performs correlations, producing a range of histograms for the user.2D4E7799-746C-4A06-A67F-BFA8CE649837First experiments of ISOLDE Solenoidal Spectrometer use this code for the online data viewing and offline analysis.622ec2308eb425.69762649trueISOLDE Solenoidal Spectrometer Sort CodeData analysis techniquehttps://github.com/ISOLDESolenoidalSpectrometer/ISSSort2021This release combines a large number of changes over the past year or so, some bug fixes and a couple of long-awaited enhancements. The main enhancements are in the ability to read simulated data from NPTool and PACE4. The histogrammer now makes a complete set of recoil time-random plots that can be used for subtraction, although the user must make the subtraction themselves. Some bug fixes included the n-side mapping, array histogram z bin limits, and some energy-loss and pulse-height-correction fixes. What's Changed Fixed missing dep for &quot;make -j&quot;. by @hanstt in https://github.com/ISOLDESolenoidalSpectrometer/ISSSort/pull/18 Silencing some compiler warnings. by @inkdot7 in https://github.com/ISOLDESolenoidalSpectrometer/ISSSort/pull/19 Try a CI action file. by @inkdot7 in https://github.com/ISOLDESolenoidalSpectrometer/ISSSort/pull/20 Add recoil E and dE eloss spectra. by @dj-clarke in https://github.com/ISOLDESolenoidalSpectrometer/ISSSort/pull/21 Extend ex histograms by @ACeulemans in https://github.com/ISOLDESolenoidalSpectrometer/ISSSort/pull/22 Print as (int) in error output. by @inkdot7 in https://github.com/ISOLDESolenoidalSpectrometer/ISSSort/pull/24 Small bug fix and improvements to energy loss and pulse-height correction calculation by @berjones in https://github.com/ISOLDESolenoidalSpectrometer/ISSSort/pull/25 B jones correct nside mapping by @berjones in https://github.com/ISOLDESolenoidalSpectrometer/ISSSort/pull/23 Bug in autocal residuals plot by @dj-clarke in https://github.com/ISOLDESolenoidalSpectrometer/ISSSort/pull/27 Fix bug #26 reported by Andreas Ceulemans by @lpgaff in https://github.com/ISOLDESolenoidalSpectrometer/ISSSort/pull/28 Revert some changes and apply correct fix for issue #26 by @lpgaff in https://github.com/ISOLDESolenoidalSpectrometer/ISSSort/pull/29 Update histogram limits for the z axis of the array by @lpgaff in https://github.com/ISOLDESolenoidalSpectrometer/ISSSort/pull/30 Make -j fix by @inkdot7 in https://github.com/ISOLDESolenoidalSpectrometer/ISSSort/pull/31 Correct the module indexing of detecors from nptool by @berjones in https://github.com/ISOLDESolenoidalSpectrometer/ISSSort/pull/32 Histogrammer updates by @lpgaff in https://github.com/ISOLDESolenoidalSpectrometer/ISSSort/pull/33 Set the Sumw2 method by default on histograms by @lpgaff in https://github.com/ISOLDESolenoidalSpectrometer/ISSSort/pull/34 New Contributors @hanstt made their first contribution in https://github.com/ISOLDESolenoidalSpectrometer/ISSSort/pull/18 @inkdot7 made their first contribution in https://github.com/ISOLDESolenoidalSpectrometer/ISSSort/pull/19 @berjones made their first contribution in https://github.com/ISOLDESolenoidalSpectrometer/ISSSort/pull/25 @lpgaff made their first contribution in https://github.com/ISOLDESolenoidalSpectrometer/ISSSort/pull/28 Full Changelog: https://github.com/ISOLDESolenoidalSpectrometer/ISSSort/compare/v2.4...v3.06DE03083-A7A1-498F-842D-A275BFD09935All data from the ISOLDE Solenoidal Spectrometer is being analysed with this software.true65f0df72ef23e9.88353140ISOLDESolenoidalSpectrometer/ISSSort: v3.0Softwarehttps://zenodo.org/doi/10.5281/zenodo.106947562024E6F46DA5-4EAD-4B45-977C-BAB68E4E308BEnhancing the performance of solenoidal spectrometers for inverse reactionsProceedings of the Royal Society A: Mathematical, Physical and Engineering Sciencesf7a09e176fdb36891561d2e2cec71c9aButler P2023-01-0114712946 1364502165e317b9cdc2f1.86060711743C3B35-A24D-45BD-AA5F-E8BE85941E1AFirst Exploration of Neutron Shell Structure Below Lead and Beyond $\boldsymbol{N=126}$9ab368e946473871205b39fa67de072fTang T2020-01-0165e946c863ade9.50608180BF3C359E-1A3E-4760-95F6-21E4A8B4DADEEvolution of single-particle structure near the N = 20 island of inversionPhysical Review C94d6c926287e6cf3c09d56c1a41f5495MacGregor P2021-01-0124699993 24699985640727d0f1ab72.457678627A24CA9F-3AE6-438A-9449-72FB0456E476TSR: A storage and cooling ring for HIE-ISOLDENuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atomsf2e00f21587f45a7ee3f3854b9215c8dButler P.A.2016-01-010168583X56debec6a083b2.937184351A4AD57B-B5B7-41FE-A6FD-05EADAB84DE3Evolution of single-particle structure near the N = 20 island of inversionPhysical Review C94d6c926287e6cf3c09d56c1a41f5495MacGregor P2021-01-0161faab83489618.1529328853211C5F-74E0-466E-BF53-8425678D6446Do nuclei go pear-shaped? Coulomb excitation of 220 Rn and 224 Ra at REX-ISOLDE (CERN)EPJ Web of Conferencesa27cee72c70dfa5e45ff5bb2f038ae1cScheck M2015-01-0158bea96d4649b9.8508199258331C84-89BD-4410-884C-039EB5B4E2BCTSR: A Storage Ring for HIE-ISOLDEActa Physica Polonica Bf7a09e176fdb36891561d2e2cec71c9aButler P2016-01-0164781a1123475E421B3F8-C753-4017-92E4-E023CDD424FDDirect Determination of Fission-Barrier Heights Using Light-Ion Transfer in Inverse Kinematics.Physical review letters4743b326a647121835a8b4b8f083caceBennett SA2023-01-010031-900764d3c1683776a0.6997417567C79801-CAC6-463D-99A4-3F2F4F89DF5DFirst Exploration of Neutron Shell Structure below Lead and beyond N=126.Physical review letters695f56f11b77725f0553d5e1b74b1ed4Tang TL2020-01-010031-90075e5636d6116ea9.62133740E4CB6475-1ADF-4D32-8A0C-2F776E838D62Direct Determination of Fission-Barrier Heights Using Light-Ion Transfer in Inverse Kinematicsa2af555eec2c3db3dbc39cd71c4262e1Bennett S2023-01-0165e918d973b159.58049975ST/M00161X/18C77C51A-5639-42DA-B4F4-4A184CA2EA00100Nuclear physics5333CE47-4782-4D31-A764-62C2F691410150Nuclear AstrophysicsA4CEBAA4-8692-41A1-89CB-E92E7854929E50Nuclear Structure00985EBB-E6BE-41BC-BF76-003FE42B0869Exploiting natural product assembly line genomics and synthetic biology for discovery and optimisation of novel agrochemicalsClosedBB/K002341/1Research GrantMicroorganisms including bacteria and fungi are everywhere in the environment. Although a few microorganisms have roles in causing disease, most microorganisms are harmless, and many of them actually produce medicines and chemicals useful to man. A good example is penicillin which is produced by a fungus and used as an effective antibiotic in human and animal medicine. Other compounds include anticancer drugs, drugs which allow organ transplants by suppressing the immune system and anticholesterol drugs. Many microbes also produce compounds of huge importance in agriculture which can be used as insecticides, herbicides and fungicides. It is estimated that around 40% of current world food productivity would be lost without these. As the world population grows and as climate change takes hold efficient food production and food security will become more important and the roles of these naturally occurring compounds will increase yet further. Penicillins came into use during the 1940s, and for around half a century research provided a steady stream of newly discovered natural products. However, traditional approaches began to fail as more and more compounds were discovered because the available methods kept finding the same known compounds. This led companies to try other avenues to provide new compounds for use as medicines and agrochemicals - however fully synthetic compounds have not proven as successful as natural products. Over the past decade academic research, much funded in the UK by BBSRC, but also an international effort, has led to the understanding that most microbes have the capacity to produce very many more compounds than observed - perhaps only 10% of a given organism's potential has been collected to-date. Genome sequencing has revealed that the biosynthetic potential of known organisms is huge - and new organisms are continually being found. If the 90% of unused genes in just the known organisms could be activated there could be a strong flow of new compounds for testing as medicines and agrochemicals - this flow could be increased to a flood if a generic technology could exploit all the as-yet undiscovered microbes. In parallel with the genome sequencing efforts huge progress has also been made in understanding the genes, enzymes and chemistry involved in the microbial synthesis of secondary metabolites. This now allows the pathways responsible for the synthesis of secondary metabolites in microbes to be engineered to produce yet more compounds. The confluence of cheap whole genome sequencing and the ability to engineer microbial pathways underpins this research proposal. The project will be a collaboration between 6 partners: the Challis group at Warwick, expert in microbial genome analysis; the Leadlay group at Cambridge, expert in bacterial polyketide biosynthesis; the Micklefield group in Manchester, expert in bacterial peptide production; the Cox group in Bristol expert in fungal biosynthesis; and Syngenta and Biotica, UK companies with major interest in secondary metabolites. We will obtain the genome sequences of bacteria and fungi known to produce agrochemically useful compounds. We will find the genes responsible for their production and recombine and engineer them to make higher amounts of these compounds, and then libraries of related compounds for testing. We will work with partners in the international agrochemical company Syngenta to develop these as new herbicides, insecticides and fungicides, while partners at the Biotechnology company Biotica will focus on compounds with use in human medicine. Overall we aim to develop a platform technology which can exploit the potential of microbes for the production of useful compounds for use in agriculture and medicine. We will also disseminate our results widely and undertake outreach activities to increase public awareness of industrial biotechnology and the role of genetic engineering and microbiology in ensuring future food security.This project will exploit a major opportunity which has arisen due to three factors: the dramatic lowering in cost of microbial full genome sequencing; the recent advances in rational engineering of microbial metabolic pathways; and the re-emergence of interest in natural products as new agrochemicals and drugs by international companies. The project will bring together 6 partners: the Challis group at Warwick, expert in genomics-based natural product discovery; the Leadlay group at Cambridge, expert in bacterial polyketide biosynthesis; the Micklefield group in Manchester, expert in bacterial nonribosomal peptide bioengineering; the Cox group in Bristol expert in fungal biosynthesis; and Syngenta and Biotica, UK companies with major interest in secondary metabolites. The collaboration will allow the 6 partners to embark on an ambitious programme to rapidly sequence the genomes of 40 microorganisms with the known ability to produce compounds with potential in the agrochemical arena. New bioinformatic methods will be used to rapidly identify biosynthetic gene clusters and link them to the synthesis of particular compounds. Engineering will then be employed to increase titres and activate 'silent' gene clusters with potential to produce bioactive compounds. Focussed libraries of target compounds will be made by biosynthetic engineering and the libraries used for SAR by Syngenta. Compound activity will then be maximised by a combination of biosynthetic engineering and synthetic chemistry. The partners will also engage in dissemination, training and outreach activities designed to maximise the impact of the project in the academic, industrial and public communities.Short term: The project will have high impact with the directly involved partners. For the companies involved it will allow them to gain access to and exploit the significant pool of knowledge and experience within the UK academic community in the area of biosynthetic engineering and synthetic biology. In particular this will help give Syngenta a competitive advantage and maintain their significant research and employment base in the UK. It will have a significant impact for the 4 academic groups involved because it will allow them to collaborate and disseminate best practice in complementary areas of research - this is likely to lead to more publications and publications of higher impact and thus help maintain the UK's competitivity in this area. It will help the academic groups focus their efforts on the development of new products and bring knowledge and experience from commerce into the academic arena. This in turn will enable the academic groups to form new and effective collaborations. Medium term: The research has a good likelihood of leading to the development of new products with utility in the agrochemicals sphere which will underpin improvements in food security internationally. The project will train at least 8 PDRAs and up to 4 students in the area of synthetic biology as applied to industrial biotechnology. These people will form a core of expertise which will benefit both academia and industry. Long term: The development of a platform technology for the systematic exploitation of microbes for the development of new medicines and agrochemicals will form the basis for the development of new technologies using synthetic biology. For example it is likely that similar methodology will underpin the development of new materials, new fine chemicals and processes, new methods to access biofuels and new methods to access foodstuffs.2018-07-30198E4A3D-B2DC-45D4-8351-7CCEC4061876BBSRC2013-07-31INCOME_ACTUAL3552006Syngenta International AGUnited KingdomSyngenta Ltd (Bracknell)Syngenta screening collaboration7714D1CD-7EA8-4E28-95F7-0089A8774BCEnone yet! yes, this multidisciplinary - combines natural products chemical biology and crop scinece.622b27368bc167.02189879-1Tested compounds supplied in herbicidal, insecticidal and fungicidal assaysSupplied novel natural products for biological testingPrivate2022-01-01Syngenta International AGUnited KingdomSyngenta Ltd (Bracknell)Syngenta matched fundingED15F549-54C4-4E1A-8004-C9F4C57A99C2The pesticidal activity has been determined of several natural products and derivatives that have hitherto never been tested by Syngenta. In some cases potent agrochemically-relevant biological activity has been observed. Discussions with Syngenta about how to further develop these compounds are ongoing. In addition, Syngenta have increased their activity in the area of natural product discovery and development, in particular through recruiting to specialist in this field who received postdoctoral and or PhD training in our group at the University of Warwick.5aa69d5b758843.58753452-1Our collaborators have have investigated the pesticidal activity of the natural products we have isolated.We have isolated and structurally characterized several tens of natural products from Actinobacteria and filamentous fungi.Private2013-01-01Schools mini-projectParticipation in an open day or visit at my research institutionRegional509511CD-D4AA-4615-9A94-C7DFDC695359&quot;Discovery of Agrochemical Natural Products&quot; practical science projects were run with groups of GCSE and A-level students from local schools in economically disadvantaged areas. This involved the students in a series of visits to our laboratories on Wednesday afternoons to carry out practical laboratory tasks and data interpretation exercises, which guided them through the steps from isolating and characterizing a new microbial strain, through assessing its potential to produce novel bioactive metabolites, to elucidating the structures of the metabolites. At the end of one of the projects, the students were accompanied by the PI/researchers/school teachers on a visit to Syngenta where the students gave a presentation on their project to colleagues in the company. The participants were also given a tour of the company's facilities. Postgraduate students from the lab were involved in the preparation and delivery of the project, in addition to the researchers employed on the project. At our grant partner Manchester, several groups of year 12 students visited for a day. Their studies and interests, as well as their future plans, were discussed. They were provided with an introduction to Streptomyces and biosynthesis of secondary metabolites with a particular focus on the wide variety of antimicrobials and agrochemicals that are of Streptomyces origin. The process of how early stage drug discovery has changed from screening to the development of genome sequencing and synthetic biology and how modern techniques are used in the lab to create bespoke compounds of interest was discussed. The students had plenty of opportunity to ask questions in an informal setting and engage with a variety of researchers from at different levels (masters students, PhD students and postdoctoral research fellows). The overall goal was to develop understanding of how life in a research lab actually operates and the exciting technologies that are driving innovation.5aa69989083ab7.69062616falseSchools2013,2015,2016121493United StatesGBPUnited Kingdomen_GBIndustrial Contract2018-09-01Achaogen0DB74A70-E887-4781-8D63-A90411A520CF5aa6923aead1a2.48027304Private2017-09-30198477United KingdomGBPUnited Kingdomen_GBGEN2NCE - a synthetic biology platform for natural product discovery2022-03-02Biotechnology and Biological Sciences Research Council (BBSRC)BB/T017163/1E784EAEF-B041-4C05-BE1F-04E00A6641CD622af61f7cbea6.67227175Public2020-07-31209834United KingdomGBPUnited Kingdomen_GBICURe Follow on Funding Grant2023-08-01Innovate UK44930DD7319F5-8BCD-41D4-8AFF-DB4C134B25A2622af7b81247f8.20269082Public2021-08-31113573United KingdomGBPUnited Kingdomen_GBNewton International Links2019-09-01British Council261846416BFEDE585-3DE2-473D-B6E5-2E26DD979DB75aa691b05c2820.12029360Charity/Non Profit2017-03-3145508212AustraliaAUDAustraliaen_AUCentre of Excellence for Innovations in Peptide and Protein Science2028-01-02Australian Research CouncilCE2001000126E632C0C-68A0-4D47-A374-5B6F459D9C93622afa9d7b8ec0.19961364Public2021-01-0110521613United KingdomGBPUnited Kingdomen_GBBBSRC/EPSRC Multidisciplinary Research Centres in Synthetic Biology2020-05-01Biotechnology and Biological Sciences Research Council (BBSRC)BB/M017982/141D32109-774E-4FBD-8F32-50046BC9EE3B5aa690f060f0e2.00651157Public2015-05-311000United KingdomGBPUnited Kingdomen_GBBBSRC-IAA2016-07-01Biotechnology and Biological Sciences Research Council (BBSRC)BB/IAA/Warwick/155458E2DA-82CB-4E4D-A68B-83F486D85D4758c81399368945.85465284Public2016-01-0151903United KingdomGBPUnited Kingdomen_GBWarwick Impact Fund2019-04-01University of WarwickD2579759-AA85-41DD-B72C-C9959551CAEB5aa69407a881c1.88667621Academic/University2018-07-3196696United KingdomGBPUnited Kingdomen_GBIPC CASE2020-09-01Biotechnology and Biological Sciences Research Council (BBSRC)BB/P504804/175FC2F76-8922-4340-B22E-0439BD82D2115aaa47526ef935.59933203Public2016-09-30Our findings are being used by Syngenta in agrochemical discovery programs. To date we have supplied them with more than 70 natural products for pesticidal screening, of which 24% showed activity. We have also received financial support from the Warwick Impact Fund to further develop some of the methods we established during the course of the project and explore their commercial potential. We have participated in ICURe, leading to the award of Innovate UK funding to establish a spin out company.2014D6A61740-D5C0-4633-A3B6-C154005982EEEconomic5464cc32244496.60457391Agriculture, Food and Drink,Manufacturing, including Industrial Biotechology,Pharmaceuticals and Medical BiotechnologyThis project has allowed us to build on earlier work to develop what appears to be a widely applicable strategy for the activation of silent biosynthetic gene clusters.BB/K002341/16DDF5F4A-5199-448C-A96B-32F9C178760EIn December 2017 we received funding form the Warwick Impact Fund to explore the commercial prospects for this strategy with a view to establishing a spin-out company. In 2019, Dr Douglas Roberts, who worked as a postdoctoral research fellow on the project, successfully completed the midlands Innovation to Commercialisation of University Research (ICURe) program and in 2020 we were awarded Innovate UK funding to set up a spin out company (Erebagen). We are currently in the process of securing matchin Venture Capital investment to enable us to set up the company.No5aa69aed03b3b0.91238917Protection not requiredA genomics-driven platform for novel bioactive natural product discovery44 Streptomyces and 10 fungal genomes have been sequenced. Numerous novel specialised metabolite biosynthetic pathways have been discovered by analysing the genome sequences. The metabolic products of numerous pathways have been isolated and sent to Syngenta for biological testing. Several pathways of interest have been experimentally elucidated. Examples include: thaxtomin A, TMC-86A, malonomycin, cycloaspeptide, strobilurin and marginolactones. The thaxtomin and cycloaspeptide pathways have also been manipulated, providing access to novel derivatives of the natural products. A wide range of methods for activating biosynthetic gene clusters in Actinobacteria that are poorly expressed in laboratory cultures have been explored, leading to the identification of a method that appears to have general applicability. This method has been applied to the discovery of a range of novel metabolites, the structures and biological activities of which are being elucidated through follow-on funding.Syngenta will take our findings forward to help develop novel crop protection chemicals. Our results also have potential to be used by the pharmaceutical and animal health industries. The novel insights into molecular mechanisms of natural product biosynthesis we have generated can be exploited to develop new biocatalysts with a range of potential applications. They can also be harnessed to develop new biosynthetic engineering strategies able to create novel natural product analogues with improved application potential. The broadly applicable method we have developed for activation of &quot;silent&quot; biosynthetic gene clusters offers significant potential for the generation of novel natural product libraries. We have explored the possibility of commercializing this with support from the Warwick Impact Fund and through a BBSRC Pathfinder Award. Following successful completion of ICURe we have secured funding from Innovate UK to set up a spinout company.A2640802-CD91-4DCD-BD01-A93C819911F85464d0044715b7.65894921Agriculture Food and DrinkChemicalsEducationManufacturing including Industrial BiotechologyPharmaceuticals and Medical BiotechnologyRelated Article: Kate M. J. de Mattos-Shipley, Catherine E. Spencer, Claudio Greco, David M. Heard, Daniel E. O'Flynn, Trong T. Dao, Zhongshu Song, Nicholas P. Mulholland, Jason L. Vincent, Thomas J. Simpson, Russell J. Cox, Andrew M. Bailey, Christine L. Willis|2020|Chemical Science|11|11570|doi:10.1039/D0SC04309E8366B1D5-9A85-4F63-A07A-6228309F56C765ddf4fbd4bd24.62298274trueCCDC 2011264: Experimental Crystal Structure DeterminationDatabase/Collection of datahttp://www.ccdc.cam.ac.uk/services/structure_request?id=doi:10.5517/ccdc.csd.cc25hwh2&sid=DataCite2020Related Article: Kate M. J. de Mattos-Shipley, Catherine E. Spencer, Claudio Greco, David M. Heard, Daniel E. O'Flynn, Trong T. Dao, Zhongshu Song, Nicholas P. Mulholland, Jason L. Vincent, Thomas J. Simpson, Russell J. Cox, Andrew M. Bailey, Christine L. Willis|2020|Chemical Science|11|11570|doi:10.1039/D0SC04309E58DB29C6-324D-42AF-B2A6-05CDF5F0130865ddf626871635.31203389trueCCDC 2011262: Experimental Crystal Structure DeterminationDatabase/Collection of datahttp://www.ccdc.cam.ac.uk/services/structure_request?id=doi:10.5517/ccdc.csd.cc25hwf0&sid=DataCite2020Related Article: Kate M. J. de Mattos-Shipley, Catherine E. Spencer, Claudio Greco, David M. Heard, Daniel E. O'Flynn, Trong T. Dao, Zhongshu Song, Nicholas P. Mulholland, Jason L. Vincent, Thomas J. Simpson, Russell J. Cox, Andrew M. Bailey, Christine L. Willis|2020|Chemical Science|11|11570|doi:10.1039/D0SC04309EA76F56D3-4954-4873-BF22-E7924397BAB265ddf8dcf0e7b5.25827124trueCCDC 2011263: Experimental Crystal Structure DeterminationDatabase/Collection of datahttp://www.ccdc.cam.ac.uk/services/structure_request?id=doi:10.5517/ccdc.csd.cc25hwg1&sid=DataCite2020ErebagenErebagen develops software that uses bioinformatics to discover compounds for pharmaceuticals development.E74E9D77-8519-440A-BBEF-8B0CB8CB61382 FTE scientific post currently in the company.622b0002732d18.848488941741863748https://erebagen.com/20202C1E0139-85EA-4C31-865E-1EC90F81924CAn Amidinohydrolase Provides the Missing Link in the Biosynthesis of Amino Marginolactone AntibioticsAngewandte Chemie4864445b19a3f452037b2b692cb0926cHong H2015-01-01585d321363cae5.67486541E0EBC70D-D828-433F-B4B0-DBED08AB56D3Evidence for an iterative module in chain elongation on the azalomycin polyketide synthase.4864445b19a3f452037b2b692cb0926cHong H2016-01-0165e8671f3cb127.629909628AE439C0-710C-4178-8C4C-2E98DE71909CA Flavin-Dependent Decarboxylase-Dehydrogenase-Monooxygenase Assembles the Warhead of a,ß-Epoxyketone Proteasome InhibitorsJournal of the American Chemical Society22846bfd2bfb90dd03356d8184e85ae0Zabala D2016-01-010002-7863585d70de752b58.02850546AC53E570-F4D5-402B-AD82-843BDB71F505Expanding the Substrate Scope of Nitrating Cytochrome P450 TxtE by Active Site Engineering of a Reductase FusionChemBioChem40d3fc27135efaa50509ac50bc139663Saroay R2021-01-011439-42276112902ad06ceD6A51379-7E66-4FBA-8B0E-6007EDB4A448De novo Biosynthesis of &quot;Non-Natural&quot; Thaxtomin Phytotoxins.Angewandte Chemie (International ed. in English)54dcab1088db15fe632408a63baab337Winn M2018-01-011433-78515b6331e1cb7864.244344704DA4685B-9A57-4313-8372-1500A5A25EB0Exploration of polyyne biosynthetic gene cluster diversity in bacteria leads to the discovery of the Pseudomonas polyyne protegencin2e7f5b487c0135a762effe38b1bc6e9aMullins A2021-01-016112902ca2c2459EAE8E9-D06E-48B5-AA09-3EEA199DF9D2An Amidinohydrolase Provides the Missing Link in the Biosynthesis of Amino Marginolactone Antibiotics4864445b19a3f452037b2b692cb0926cHong H2016-01-0165e865183f3985.602635919AB1A2A5-1137-4646-881B-8A9FA361AE96Harnessing and engineering amide bond forming ligases for the synthesis of amidesCurrent Opinion in Chemical Biology54dcab1088db15fe632408a63baab337Winn M2020-01-011367-59315f80a77c132624.5348534496D899CC-EFF6-4167-A484-8B05161F6346The cycloaspeptides: Uncovering a new model for methylated nonribosomal peptide biosynthesisee910d8e75e3adaa24139924b5d3c5bfDe Mattos-Shipley K2018-01-0167520d54ba17d3.17961550409A957E-0E68-46BC-96A4-A137220A552BHeterologe Produktion pilzlicher Maleidride enth&uuml;llt die kryptische Cyclisierung in ihrer Biosynthesec06da4926bbf05c100ed251f0d7ec1faWilliams K2016-01-0167520daa8bc469.08262818D3C434DE-9E76-444D-A672-708AF42AB741Evidence for an iterative module in chain elongation on the azalomycin polyketide synthase.Beilstein journal of organic chemistry4864445b19a3f452037b2b692cb0926cHong H2016-01-011860-53975f2002281dd1e2.0909390296477BD3-F3F8-4924-B782-9143186DF1E9Heterologe Produktion pilzlicher Maleidride enth&uuml;llt die kryptische Cyclisierung in ihrer BiosyntheseAngewandte Chemiec06da4926bbf05c100ed251f0d7ec1faWilliams K2016-01-0165bb7ad10ba816.13165708C64CC144-FDC9-4B79-8D03-B9D426A8B26DGenomics-Driven Discovery of a Novel Glutarimide Antibiotic from Burkholderia gladioli Reveals an Unusual Polyketide Synthase Chain Release MechanismAngewandte Chemie International Editionb7715f91ed30555ad08314c9c7795ecbNakou I2020-01-011433-78515fb5662ad8d5d8.17622220B1B5A062-8764-4352-9670-69C92A176823An unusual Burkholderia gladioli double chain-initiating nonribosomal peptide synthetase assembles 'fungal' icosalide antibiotics.Chemical sciencefebd0f2c18e989c5205c193f9de5a8e6Jenner M2019-01-012041-65205d91d8162df310.5747403255765730-5CC8-4242-BCEB-DC089D717C50Discovery of microbial natural products by activation of silent biosynthetic gene clusters.Nature reviews. 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S816: Cytochrome P450-Catalyzed Installation of the C-14 Hydroxyl Group.ACS chemical biologybf718ff7d1450b2d6d791149cba67dccZhou S2019-01-011554-89295d91d75e6c0cc2.71010503BDE723F8-A35E-4BCD-BCB3-9EE63C57B1B9Strobilurin biosynthesis in Basidiomycete fungi06b0e1b757517bb008e9941eeeb55086Nofiani R2018-01-0167520f437077a0.77047155DF0AC94D-1301-44D8-A765-914DF91E0B11Uncovering biosynthetic relationships between antifungal nonadrides and octadrides.Chemical sciencea065d2a3a0ca48fafa1e6dc8a89f998fde Mattos-Shipley KMJ2020-01-012041-65205f80ac8ea34ae7.915335266D60480E-98E1-4C97-A474-E36B4785AA48Discovery and Biosynthesis of Gladiolin: A Burkholderia gladioli Antibiotic with Promising Activity against Mycobacterium tuberculosis.fe0c303eb11e18ccfbeebde200a22b90Song L2017-01-0165e8698c07ae23.37636154B0241CF0-B00C-4D41-AD6F-D34CB7460FC2Watasemycin biosynthesis in Streptomyces venezuelae: thiazoline C-methylation by a type B radical-SAM methylase homologue.Chemical sciencebb81dad617a4110bfd4afe8a50fb1b19Inahashi Y2017-01-012041-65205aa68d7f229df3.23067879C9B94DB7-1507-4BB1-AE11-A4F85B4A0ED4A dual transacylation mechanism for polyketide synthase chain release in enacyloxin antibiotic biosynthesis.dd992b9cd11a41174e3e501cdc3a7b21Masschelein J2019-01-0165e885c46df807.1091247403FB72BF-E5FA-415B-BE3F-7FDF315CCFF5Antibiotic Skeletal Diversification via Differential Enoylreductase Recruitment and Module Iteration in trans -Acyltransferase Polyketide SynthasesJournal of the American Chemical Society8a4aae6c6d55bed2162309db037d0147Jian X2024-01-0165db75e04fb069.922718296FE8B24E-4FF3-4EC0-A33E-8E20D5FFAEEBMmfL catalyses formation of a phosphorylated butenolide intermediate in methylenomycin furan biosynthesis.Chemical communications (Cambridge, England)bf718ff7d1450b2d6d791149cba67dccZhou S2020-01-011359-73455fb8b7514885d5.77901458A8FEDD81-6DA4-41BD-A3C8-4242A7E77694A combination of polyunsaturated fatty acid, nonribosomal peptide and polyketide biosynthetic machinery is used to assemble the zeamine antibioticsChemical Sciencedd992b9cd11a41174e3e501cdc3a7b21Masschelein J2015-01-012041-6520585d408864fea3.79105557B2827473-454E-43CF-8D52-405BA478C017A vitamin K-dependent carboxylase orthologue is involved in antibiotic biosynthesisNature Catalysis08304709959b4c934153eb730f5547a3Law B2018-01-01252011585c8793ff6c0395.648009941C2A6009-DEC0-4B52-AE6A-25157E667F87Heterologous reconstitution of the biosynthesis pathway for 4-demethyl-premithramycinone, the aglycon of antitumor polyketide mithramycin.Microbial cell factories22846bfd2bfb90dd03356d8184e85ae0Zabala D2020-01-011475-28596030769d7f0cc93A42C75-3F86-4136-A91D-4A51C5616EC4Discovery of the Pseudomonas Polyyne Protegencin by a Phylogeny-Guided Study of Polyyne Biosynthetic Gene Cluster Diversity.mBio6a234e45e5f6e33f898094e9d0f41645Mullins AJ2021-01-0161f707148fef15.24788849B12A0EB9-1B93-4057-AED2-5D162EE7BC57Modern Biocatalysis - Advances Towards Synthetic Biological Systems8b1691ccbba8d85e8e4e149b4cc32467de Mattos-Shipley K2018-01-0117576733 1757672567ce5a68c1abd9.35260243BA0B462B-7F99-402D-A74D-EC590FCEC0A1The cycloaspeptides: uncovering a new model for methylated nonribosomal peptide biosynthesisChemical Science8b1691ccbba8d85e8e4e149b4cc32467de Mattos-Shipley K2018-01-012041-65205b633129244bf3.1510416126E2FB01-90A8-4176-BC05-889694CB8C1EStrobilurin biosynthesis in Basidiomycete fungi.Nature communications06b0e1b757517bb008e9941eeeb55086Nofiani R2018-01-012041-17235c471302926818.446115994839D76D-9086-4E4F-A697-54263F4FCAD2A dual transacylation mechanism for polyketide synthase chain release in enacyloxin antibiotic biosynthesis.Nature chemistrydd992b9cd11a41174e3e501cdc3a7b21Masschelein J2019-01-011755-43305d91d815ed2f06.3902979575846E84-00AD-441B-9C92-2F86F1B03961In silico analyses of maleidride biosynthetic gene clustersc06da4926bbf05c100ed251f0d7ec1faWilliams K2021-01-01675212d358e407.17726153A85A7F90-2407-4BD8-B28B-A3786D038BB4Pentamycin biosynthesis in Philippine Streptomyces sp. S816: Cytochrome P450-catalysed installation of the C-14 hydroxyl groupbf718ff7d1450b2d6d791149cba67dccZhou S2019-01-016112904b640c89C1FD5E5-57B4-43DA-92F7-FF3909ACCE68Sulfation and amidinohydrolysis in the biosynthesis of giant linear polyenes.Beilstein journal of organic chemistry4864445b19a3f452037b2b692cb0926cHong H2017-01-011860-53975c8793769bde02.4257370680BB8733-F07D-49BC-A102-A6F95AABBE81Parallelized gene cluster editing illuminates mechanisms of epoxyketone proteasome inhibitor biosynthesis.Nucleic acids researchc5b4ef969040b66fb3a85f89c05a4178Huang C2023-01-010305-104863ee91a0b81db9.077861070DB1A2CC-B3FF-46F1-9303-657628EBE2CFDiscovery and Biosynthesis of Persiathiacins: Unusual Polyglycosylated Thiopeptides Active Against Multidrug Resistant Tuberculosis.ACS infectious diseases5b9622bdb49697ee84fb5afb10a655c3Dashti Y2024-01-012373-8227673f13b404d130.31381232C21FF5DF-2925-431B-8F46-F5FEC61D439DDiene incorporation by a dehydratase domain variant in modular polyketide synthases.Nature chemical biology7d0da7c719661bbb1c10d204bae4a2d7Hobson C2022-01-011552-4450632e2f6945a536.186499303CBA3E18-4EFB-4E87-9B1C-26755D866606Heterologous Production of Fungal Maleidrides Reveals the Cryptic Cyclization Involved in their Biosynthesisc06da4926bbf05c100ed251f0d7ec1faWilliams K2016-01-0165e866b906be69.07864524BDFE4887-95DF-49F1-9C2C-FB5B84921E18Thioester reduction and aldehyde transamination are universal steps in actinobacterial polyketide alkaloid biosynthesis.Chemical science3defeee07068f56f51d10959ce4df990Awodi UR2017-01-012041-6520585d38e84fb738.80117312BFB37682-7ABE-4021-A583-021CA6B316FFBovistol B, bovistol D and strossmayerin: Sesquiterpene metabolites from the culture filtrate of the basidiomycete Coprinopsis strossmayeri.PloS one43e87eac6e1838b948a58356ea5ac353Banks AM2020-01-011932-62036030769689ff62BC9C45E-84FD-4E96-AFBC-C43B77BD3D01Structural basis for chain release from the enacyloxin polyketide synthase.Nature chemistryc707e3035af2c42c4107fa1fe19b5d98Kosol S2019-01-011755-43305d91d8165a1174.40214075B3022FE6-D7A8-4866-A985-F61C99CB244DHeterologous Production of Fungal Maleidrides Reveals the Cryptic Cyclization Involved in their Biosynthesis.Angewandte Chemie (International ed. in English)c06da4926bbf05c100ed251f0d7ec1faWilliams K2016-01-011433-785165bb7ae901edb2.43676215B634DD5D-8F93-415B-80FA-8A6BFD02DADBAntibiotic skeletal diversification via differential enoylreductase recruitment and module iteration in trans -acyltransferase polyketide synthases8a4aae6c6d55bed2162309db037d0147Jian X2023-01-0166450464c2d5264580204-839C-499E-AE1B-D4DF7839AB1FDiscovery and Biosynthesis of Gladiolin: A Burkholderia gladioli Antibiotic with Promising Activity against Mycobacterium tuberculosis.Journal of the American Chemical Societyfe0c303eb11e18ccfbeebde200a22b90Song L2017-01-010002-78635aa6739f290a93.406051487413E550-21D1-41E3-AC79-91ED6C6F30BAGenomics-Driven Discovery of a Novel Glutarimide Antibiotic from Burkholderia gladioli Reveals an Unusual Polyketide Synthase Chain Release MechanismAngewandte Chemieb7715f91ed30555ad08314c9c7795ecbNakou I2020-01-015fb8b3ac263cb7.1750763969B7C5A1-CCC4-403E-96E7-B82549282F1AIn silico analyses of maleidride biosynthetic gene clusters.Fungal biology and biotechnologyc06da4926bbf05c100ed251f0d7ec1faWilliams K2022-01-012054-308562763048156042.1494992274050D2A-A16A-47D5-B49D-1945F147124DDe novo Biosynthesis of &quot;Non-Natural&quot; Thaxtomin PhytotoxinsAngewandte Chemie54dcab1088db15fe632408a63baab337Winn M2018-01-015b632bcf0cdb81.09367939C7FCFE91-3B6E-47F3-A4EC-10B00142A3BFAn Engineered Tryptophan Synthase Opens New Enzymatic Pathways to ß-Methyltryptophan and Derivatives.Chembiochem : a European journal of chemical biology706be9ea1e6966816beafbcff9e4ce04Francis D2017-01-011439-42275aa682b155fe97.231271284E6EE3AC-3C0B-455D-9BA9-FEF98221C863C-Nucleoside Formation in the Biosynthesis of the Antifungal Malayamycin A.Cell chemical biology4864445b19a3f452037b2b692cb0926cHong H2019-01-012451-94485f1ff90c2f6028.93140764A23C5ADE-3B91-4C4D-9D93-3E8C7FFDB967Anti-microfouling Activity of Glycomyces sediminimaris UTMC 2460 on Dominant Fouling Bacteria of Iran Marine Habitats.Frontiers in microbiology6c108ca8e93a720c4dff5ebd3c498f07Heidarian S2018-01-011664-302X5c87b90851db76.054413757F5FA26E-6D84-40D7-8534-151A5059FD99Discovery, characterization and engineering of ligases for amide synthesis.Nature54dcab1088db15fe632408a63baab337Winn M2021-01-010028-083665a6f9016279c9.78543990C7A10A36-DE06-42F3-ABD8-41C89E45E1DFAdditional file 1 of In silico analyses of maleidride biosynthetic gene clustersc06da4926bbf05c100ed251f0d7ec1faWilliams K2022-01-0165e873146ecbf9.63654313BB/K002341/12D9083F0-05FA-4726-9EB2-3FCC293CAAF960Biomolecules & biochemistry4CCA4C04-0C28-41BE-8869-FA6391A7F00540Microbial sciences6997E843-AD07-4064-B67D-D4A928309DB440Biochemistry & physiology6D0F40FF-D03E-4429-A764-185BC521A84060Catalysis & enzymology00A9E48C-4629-44EF-B21A-00C8EFB2709CEGOR: Environmental Guidelines: Opportunities and RisksClosedAH/G015198/1Research GrantThis research cluster will bring together a team of key professionals, academic researchers representing AHRC/EPSRC disciplines as well as heritage practitioners to appraise the costs and risks of current environmental guidelines for cultural heritage in response to a changing climate. This theme has a national and international dimension since climate change, energy consumption, visitation and pressures for greater access to collections will continue to make considerable demand on cultural heritage in the 21st century globally. The scale and pace of these changes are posing unique challenges to managing the long-term preservation of material culture and are the focus of discussion amongst professional communities both nationally and internationally.This research cluster will inform this debate.\n\nCurrent environmental parameters and tolerances set out in national and international guidelines and standards as well as Governmental Sustainable Development Targets play a critical role in shaping practices in the cultural heritage sector such as building construction, and environmental management. This includes the control of temperature, moisture, light and pollution - the main factors affecting the conservation of material culture. Environmental guidelines impact significantly on how collections are stored, accessed, loaned and displayed. \n\nEqually, the cultural heritage sector is not immune from the challenges posed by global responsibility: reducing reliance on fossil fuels, changing behaviours in favour of re-use and alternative energy sources, for example. It is within this context the appropriateness of current environmental guidelines designed to meet an agreed standard for managing material culture change, enable visitors to access and experience collections to a seasonal standard of comfort, and provide access to collections both locally and internationally is being questioned as the 'costs' of this are being realised. Unfortunately, there are no easy or headline-grabbing answers to this problem: the risks need to be identified, the costs understood, the options appraised. \n\nEGOR will provide the necessary framework to develop thinking in this area in order to realise an intellectual step change in understanding the risks and uncertainties of current environmental guidelines, standards and targets in a changing climate. Consideration will be largely focused on indoor environments, collections and the people who engage with and work in the cultural heritage arena, and will build on foundations established by other research projects e.g. Noah's Ark (EU), Engineering our Futures (EPSRC), Living with Environmental Change (NERC) largely focused on climate impacts outdoors. This will be achieved through 5 sequential activities: \n1. An inaugural meeting of the steering group which includes professional leaders, and named investigators to shape thinking and initiate cross fertilisation of ideas and perspectives;\n2. 3 working group meetings comprising specialists in art history, engineering, material science and conservation for coherent discussion, and lively debate to understand the implication for current environmental guidelines in a changing climate for people, their values and history, buildings housing collections (often historic structures themselves) and collections. The implications will be considered against a background of global responsibility.\n3. A two-day residential event will conclude this investigative process; the three working groups will present their findings, areas of convergence and divergence will be further debated to determine the risks and uncertainties surrounding environmental guidelines and standards in a changing climate, and the outstanding research needed to fully inform this debate.\n\nA summary of the challenges and user-led research emerging within this theme will be reached at the end of the meeting and presented at the Programme conference in July 2009.2009-12-311291772D-DFCE-493A-AEE7-24F7EEAFE0E9AHRC2009-01-01INCOME_ACTUAL24367The findings of this award led to the publication: British Standard Publically Available Specification: 198 Specification for Managing Environmental Conditions for Cultural Collections201262B62E2B-E38F-4658-B4E5-6DDB9157CD13CulturalEconomicPolicy & public services56e006140e5968.17907723Education,Energy,Culture, Heritage, Museums and CollectionsThis project identified what was needed to deliver improved environmental standards for the movable heritage sector.The findings of this research network led to the development of a British Standards published Publically Available Specification for Environmental Management of Cultural Heritage Collections. This standard has been recognised internationally as a ground breaking.2BAB7145-8999-45B6-A169-AD64E655841254635a41d58dc5.45769760EnergyEnvironmentGovernment Democracy and JusticeCulture Heritage Museums and CollectionsOtherhttp://www.nationalarchives.gov.uk/archives-sector/assessing-environmental-impact.htm02B3D6CC-6780-47EE-B6F0-C6EF6552B7B8Volatile aldehydes in libraries and archivesAtmospheric Environment64a7586467e5c699a5202ec20c2b2efaFenech A2010-01-01doi_53cfe5fe569b88ddAH/G015198/12693EEB1-BEF5-45C6-999E-D56488EFFAD7100Cultural & museum studies77E870DC-ED98-44DF-A0C8-06A874EB34A2100Cultural Studies & Pop Culture00C808C1-2360-4DF3-9CC0-008A1DA7F5D6A modular sea lice removal system utilising electro-anaesthesia, low-pressure pumping and waterjets to gently remove lice without inducing stress and compromising fish welfare.Closed10057221EU-FundedElectrolicer will be the first system that can combine sea lice removal with fish welfare during treatment. The system has been designed by Ace, a company with extensive experience in electro-anaesthesia. Water is inserted into the pipeline to create suction that draws fish calmly from the cage into the tube. Electrolicer then holds the fish in a calming low-voltage electric field that relaxes the muscles, and low-pressure water gently removes lice and eggs. Electrolicer also reduces expenses associated with adopting this technology. The solution can be used on an existing, generalised work boat, instead of requiring the purchase of a dedicated sealice removal vessel. By avoiding chemicals and favouring multi-modal treatments within the system, Electrolicer also addresses treatment resistance in lice. Ace is backed by sustainable investment group Aqua-Spark, whose mission is to invest in companies using technology to improve sustainability in aquaculture2025-10-31240CEBFD-1052-4EAC-88DF-D88A163D61C8Horizon Europe Guarantee2023-01-01INCOME_ACTUAL1092637100572216CFA1E1F-F25C-4C23-8FE1-C47AE53E333EUnclassified00CDCA79-8C71-453A-AA24-0181FDBB9BC6How do cells shape and interpret PIP3 signals?ClosedBB/I003916/1Research GrantMulti-cellular organisms rely on a large array of different transmitter substances to allow certain cells to control the behavior of others. The more sophisticated the organism the more complex the cell to cell communication. In mammals this language probably involves hundreds of fundamentally different types of transmitter. Clearly such systems need a large collection of specialized receptor molecules that can detect the individual presence of any particular transmitter. Further, these receptors, typically found on the outer surface of the cell's limiting membrane, have to signal their specific stimulation by passing a molecular message into the cells interior, effectively informing the cell that the receptor has been activated. Clearly, if a cell has many different types of receptors on its surface the molecular signal generated inside the cell by each different receptor (often called an intracellular message) must identify and distinguish which specific receptor has been stimulated. Otherwise the cell could not discriminate between the transmitters present on the outside of the cell and could not respond correctly. Hence, mammalian cells have vastly complex intracellular signalling mechanisms continuously informing the cell of what is happening in other parts of the organism or its environment. One such intracellular signalling molecule or 'message' is PIP3. It is a phospholipid molecule found on the inside surface of the cell's limiting membrane. Levels of PIP3 rise rapidly on activation of a large number of receptors. This is surprising given the problems the cell faces in knowing precisely which receptor has been activated when it detects an intracellular signal. This grant application is to understand how it is possible that rises in PIP3 can encode specific messages from so many different receptors. We have performed some experiments that have, in fact, shown that PIP3 in cells is not a single type of molecule. At least four tiny variants of PIP3 can be detected, called molecular species of PIP3. Interestingly, we find that these different molecular species of PIP3 do not respond equivalently to different ways of activating the cells we work with. We and others have also found that the different receptors can make the levels of PIP3 rise for different times and to different maximum levels. We propose that these small differences are very important inside the cell for discriminating whether a certain receptor has been stimulated. This is a 'clever' economy or efficiency on the part of the cell and allows it to use similar mechanisms to perform many different jobs. Although on the surface these might appear trivial details in the business of understanding biology, it has recently been discovered that many different cancers are caused by mutations in genes that regulate PIP3 levels in cells. Mutations that by chance cause the production of PIP3 to be increased without any need for receptor stimulation make cancers much more likely to occur. Mutations that by chance stop the enzymes that normally break down PIP3 from working also make cancer more likely to occur. As a result it is clear that understanding how PIP3 is made and then interpreted by cells is crucial for us to better understand how cancer occurs and how to treat it. Many companies are already trying to design drugs that will reduce PIP3 levels to fight cancer. This work will help us understand how to make better drugs of that type.This proposal is a collaboration between biochemistry groups at BI and mathematical biologists at the EBI to achieve a detailed and quantitative understanding of a major mammalian signal transduction pathway, the PI3K network. Several PI3K isoforms exist in cells that can be selectively engaged by a variety of cell surface receptors to generate the membrane phospholipid PIP3. PIP3 is the initial signal, which is then transduced by 10-50 effector proteins into the regulation of complex cell responses, such as cell growth and movement. Our strategy is to focus on collecting robust, high quality data sets in a panel of isogenic, non-transformed breast cell lines (MCF10a) in which key endogenous components of the pathway can be manipulated and to embed iteration between experiment and modelling to arrive at a more satisfactory explanation of: 1) the key factors which shape the magnitude and spatiotemporal properties of PIP3 signals in response to hormonal stimulation (EGF, insulin, LPA) and oncogenic mutation; 2) The way in which different PIP3 effectors interpret these PIP3 signals and 3) the relative importance of individual PIP3 effectors in delivering regulation of chemokinesis, growth and global transcription. We plan to use homologous gene targetting, siRNA suppression and pharmacological inhibition of pathway components and measure the impact of these perturbations, in several relevant cellular contexts, on i) the levels of PIP3 and other phosphoinositides measured by a novel, quantitative mass spectrometry assay that allows systematic analysis of fatty acid composition; ii) the activity and spatial distribution of several PIP3 effectors (in some cases via knock-in of endogenous GFP-fusion proteins); iii) chemokinesis, markers of growth and global transcription (using next generation sequencing). Models will be built at several levels in the pathway and integrated to allow a deeper understanding of this network and guide more effective therapeutic intervention1) Identify the beneficiaries of this work. See the section 'the beneficiaries'. To restate, ignoring our proximal research community, they would include, within the life-time of the grant; (a) an international and broad group of commercial and academic researchers, (b) the BBSRC, our host Institutions (Babraham and EBI), (c) the post-doc researchers on the grant through the training they receive and (d) our IPA partner Astra Zeneca. In addition, in the longer term, (e) the health care sector, patients and the UK's economic competitiveness. 2) How would they benefit? a) From the technologies and approaches we propose to apply in this application. Most signficantly the lipidomics strategies we have developed to enable sensitive, medium through-put analyses of the different molecular species of PIP3. This advance enables the development of potentially direct read-outs of the effectiveness of PI3K inhibitors in a clinical setting, through the opportunity to take frozen cell or tissue samples and sensitively and quantitatively analyse their PIP3 content. Until now companies have relied on surrogate read-outs of PI3K activity that have a variety of technical and intellectual weaknesses. This approach will also change the phosphoinositide research community, historically there has ben a huge pausity of data in the field through the technical or financial challenges in capturing this type of data. This has severly limited attempts to model this pathway. b) See beneficiaries section. The BBSRC would gain from delivery of their objectives in their recently formulated strategic plan, specifically in the Bioscience for Health priority. As an IPA application, this project has evidence of its commercial relevance as 'basic research underpinning the pharmaceutical sector'. Its use of modelling is in line with the BBSRCs drive to change the culture of modern biology towards being more mathematically based. c) Both the EBI and BI have internationally competitive research environments where the post-docs would learn within a project that has both direct commercial relevance, substantial contact time with a major pharmaceutical company and a multi-disciplinary approach. d) Will benefit from accelerated access to a internationally competitive grouping working in a field in which AZ have direct interest in their inflammation and oncology programmes and from having first option on any IP that might emerge from the project. More specifically they will gain direct leverage from results with the cell lines and inhibitors we have chosen to focus upon; both are used within their own, internal, research programmes. They will also gain early insights into the lipidomics approach we have developed and how they might use it to solve long-standing problems with bio-markers of activity in the PI3K pathway in whole animal or clinical studies. e) These could be longer terms outcomes resulting from improved focus on the most appropriate PI3K targets and hence the best PI3K-selectivity profile of potential drugs in specific therapeutic setting and the development of more relevant and useful bio-markers. As our IPA partners oncology programme is based in the UK (Alderly Edge, Macclesfield) the above should give competitive advantage to UK business. 3) How would we ensure the above potential benefits are realised a) Presentations at international science meetings, publications, good data sharing practice, seminars at companies b) Through the projects funding and execution c) Regular meetings between EBI and BI labs and with AZ researchers (see work plan) d) Through (c) and see Case for support e) The IPA status of this application and the success of past research collaborations (&pound;300K) with AZ are based on our approach to, and conduct within, collaborations with industry. AZ have confidence we will put effort into transferring knowledge and skill and that it will give them competitive advantage in getting good therapeutics into the market-place.2015-10-04198E4A3D-B2DC-45D4-8351-7CCEC4061876BBSRC2011-05-30INCOME_ACTUAL1086512Akita UniversityJapanSasaki_ mass spec and prostate0C7CCAAD-08CE-403A-8236-00D2407C695FNorton et al (2016) Adv Biol Regul. 60:36-45. doi: 10.1016/j.jbior.2015.10.005 Ferguson et al (2007) Nat Cell Biol. 9(1):86-91. Malek M, Kielkowska A, Chessa T, Anderson KE, Barneda D, Pir P..... Stephens LR, (2017). PTEN Regulates PI(3,4)PSignaling Downstream of Class I PI3K.. Molecular cell, 68 (3), pp. 566-580.e1058b5b5498fbad4.10061619-1Intellectual input, reagents, mass spectrometric methods, personnelIntellectual input, reagents, mass spectrometric methods, personnelAcademic/UniversityUniversity of TorontoCanadaSergio_Grinstein_SopBCE0FBBA8-ADEB-47E2-9E14-D2A89D8B37A2Manuscript currently in Press at Nature Cell Biology622209a4e34a73.10799339-1They lead the project, providing the intellectual framework and the vast majority of the data.We used our mass spectrometry methods to define a new phosphoinositide reaction mechanism for the Salmonella effector protein SopBAcademic/University2021-01-01University College LondonUnited KingdomDivision of BiosciencesNick Luscombe29D0F97F-8508-4DCE-ADA4-FC9526A4839DPublications, a number are on-going. Multi-disciplinary, biochemistry, computer modelling, bioinformatics, biological chemistry/mass spec development.546373be5e37e1.52808872-1Training in bioinformatic techniquesWe provided data, knowledge and experienceAcademic/University2012-01-01AstraZenecaUnited KingdomAZ/Cosulich090F6EBA-9E38-4B42-B4DD-4C3A851075C5Papers and models (still in development), much work is currently being written-up.546485a40f8d63.36861866-1Insights in to commercial directions and interests in this area.We are measuring and modelling agonist and onco-mutant driven changes in PIP3 signalling and then using selective inhibitors and RNAi to understand how the PIP3 signalling network operatesPrivate2009-01-01University of TorontoCanadaSergio_Grinstein_SopB9E07E6FD-8E34-44B5-BAF0-387BD5454D4FManuscript currently in Press at Nature Cell Biology622209a4e34a73.10799339-2They lead the project, providing the intellectual framework and the vast majority of the data.We used our mass spectrometry methods to define a new phosphoinositide reaction mechanism for the Salmonella effector protein SopBAcademic/University2021-01-01Work experience for year 12 school studentsParticipation in an activity, workshop or similarLocalF7934977-EA08-4F43-838C-0B471103BF96Work shadowing for a yr 12 school student to understand biomedical research Student applied and got an offer on a biomedical degree course54624afb870e31.83032049falseSchools2014Training other researchers in use of lipidomics techniques.Participation in an activity, workshop or similarInternationalCDBA87F2-6CBB-4034-85CE-6A4EF8274BB6Researchers visit BI for 1-5days typically to observe, discuss or trouble shoot lipidomics techniques. PhD students, PIs, technicians and commercial staff have been involved. Researchers from Japan, France and UK.56c1b05185e4f0.04081206falseProfessional Practitioners2012,2013,2014,2015Cambridge Science FestivalParticipation in an activity, workshop or similarRegional2A063BCF-761B-4402-97AF-CB6D661589DDExhibit at Cambridge Science Festival was attended by all age groups with extensive interaction and discussion56d8ce6350d1e8.87454856falsePublic/other audiences2015Public engagement ageing meetingParticipation in an activity, workshop or similarRegional2D0FF3F2-AA08-48BE-97B7-8763708C8C90A meeting held at Cambridge Guildhall with brief presentations from a panel of mixed scientific backgrounds followed by Q&amp;A and group discussions; also practical demonstrations of how reaction times, hearing, etc changes with age56d8cee11d7279.62768293falsePublic/other audiences2012Invited lecturer to international meetings (average 2-3 per year)A talk or presentationInternational85086E79-6246-415F-BDE4-BB4CB1F089E6promoted discussions, collaborations scientific collaborations, joint grants and publications546215ce564be0.43637759falseProfessional PractitionersPre-2006,2006,2007,2008,2009,2010,2011,2012,2013,2014,2015,2016,2017,2018,2019To determine the isoform of PI3Ks that are best targetted in different forms of cancer. Previous dogma and much pharmaceutical investment had being based on the concept that in tumours lacking the tumour suppressor PTEN PI3Kbeta was selectively augmented and the primary therapeutic target. Part of our study showed this is untrue. This has changed biotech PI3K targetting strategy and funding. We have shown that a well known tumour suppressor (an inhibitor of tumour progression) is a PI(3,4)P2 phosphatase in addtion to its other known roles, this has important implications for cancer progression and understanding how different mutations in cancer interact and can influence therapeutic strategy. Further work flowing from this award went to show that PTEN, a human tumour suppressor and PIP3-3-phosphatase is also a PI(3,4)P2 3-phosphatase and this is important for its cellular functions and this has led to new strategies to inhibit and modulate PI3K pathway activity.20145578A926-A7F0-41D0-8B1F-667D83B9E24FEconomic56bb02289579c5.29857628Healthcare,Pharmaceuticals and Medical BiotechnologyWe have developed a new method to measure PI(3,4)P2 a critical intracellualr signal. We have shown PI(3,4)P2 is degraded by an enzyme that is a key inhibitor of tumour progression and when the tumour suppressor is removed, and a potentially affected person becomes much likely to get cancer, this is in part because the levels of PI(3,4)P2 signal in cells becomes dangerously high. We have developed tools to analyse large complex changes in mRNA accumulation in response to genetic and pharmacological manipulation of PI3K activity. We have clarified mechanisms of PI3K regulation that are relevant to therapeutic strategy in cancer trails and treatment.Our basic-biology results have already changed companies strategies in targeting specific cancer types with specific PI3K inhibitors.05653636-6376-4A8B-99CC-B604FEFDE81D5463702eb55f51.13006653HealthcareTo accompany article published in Molecular cellAE3BF705-1CD1-4E1E-B66A-13CBE47EB27D65dde63762b4a4.87758778truePTEN regulates PI(3,4)P2 signalling downstream of Class I PI3KDatabase/Collection of datahttps://data.mendeley.com/datasets/tnj6m88k6w/12017In collaboration with Jonathan Clark in the Biological Chemistry Facility at the Babraham Institute we have developed novel mass spectrometry approaches for measuring different molecular species of phosphoinositides in cells and tissues. This work has utilised chemical derivatisation coupled to LC-MS and involved the synthesis of several isotope-enriched internal standards.3570B4CB-13B7-4548-AFDD-38BEAD684EB5The main impacts have been numerous publications (&gt;30 as of 2021), collaborations and clinical trials of PI3K inhibitors.622203963dc4b7.38742910trueMass_spectrometry_analysis_of_phosphoinositidesTechnology assay or reagenthttps://www.babraham.ac.uk/science-services/biological-chemistry/jonathan-clark2011F3486254-5DBE-4DF2-A58A-D90D182ED3F3Emerging evidence of signalling roles for PI(3,4)P2 in Class I and II PI3K-regulated pathways.Biochemical Society transactions9d3df4506683f973bcc340d59b4b4a5eHawkins PT2016-01-010300-512758b439901d0341.87001628B13F09C7-275F-4248-9397-AB2E847D3385A new approach to measuring phosphoinositides in cells by mass spectrometry.Advances in biological regulationbc631ac334819daffd5622aa3c7c24a6Kielkowska A2014-01-012212-49265463715f8e64a2.18675920087B5F19-313F-488E-9BAE-43AEBC597DBBPIP-ing Lipids on Membranes: PTEN Takes the Cake.Molecular cell3f910d0addbd5b7d25d64a230f01a74cRavi A2017-01-011097-276567520f44d96161.58607893927DF51A-7399-4A90-95B8-9D0D3A28328CBMX acts downstream of PI3K to promote colorectal cancer cell survival and pathway inhibition sensitizes to the BH3 mimetic ABT-737.Neoplasia (New York, N.Y.)15b2aa803b28c58fa133b58ee608eaaePotter DS2014-01-011476-558656bb048eb1ca51.751815054A2125CC-E6A5-4AFF-A6CD-2D7CCB344131Perturbations of PIP3 signalling trigger a global remodelling of mRNA landscape and reveal a transcriptional feedback loop.Nucleic acids research51585f72da0559dba579b0473aa0f917Kiselev VY2015-01-010305-104856b0cdd91ead15.0921803502AB8E6D-914E-4B9E-B67D-AA4346B3A319Frontline Science: TNF-a and GM-CSF1 priming augments the role of SOS1/2 in driving activation of Ras, PI3K-?, and neutrophil proinflammatory responses.Journal of leukocyte biology7f84660b423d6c09271cb9f398505849Suire S2019-01-010741-54005c7d43ac1cf6e4.11278104F25B140F-E040-47F7-9CF9-D64D7DCB7D6FPTEN Regulates PI(3,4)P2 Signaling Downstream of Class I PI3K.Molecular cell32166a1af7e300ccef30545562aeb163Malek M2017-01-011097-27655a8af658dee1a5.016037189E1BF81D-20EF-4CE8-8CC8-9BE3F65F2F18Signaling via class IA Phosphoinositide 3-kinases (PI3K) in human, breast-derived cell lines.PloS one335ea1a5172fc6d0d647e3c40642d987Juvin V2013-01-011932-62035463715fc2e9c6.132497424C3C5BCD-102E-401E-B052-548A4BC83F32Profiling of phosphoinositide molecular species in human and mouse platelets identifies new species increasing following stimulation.Biochimica et biophysica acta. Molecular and cell biology of lipids81f01c0fef8f0f705aa63e4abb959f27Mujalli A2018-01-011388-19815c7d421b71a352.7467192502A735FA-93CC-44AA-992C-D0EA145F9DF7Quantification of PtdInsP3 molecular species in cells and tissues by mass spectrometry.Nature methodsedc5f7c0e58dfe61e7f81d9f8d2b7544Clark J2011-01-011548-70915463715d78f491.688228351B163317-F17E-48AD-A999-D7AB1B7AB15ALysophosphatidylinositol-acyltransferase-1 (LPIAT1) is required to maintain physiological levels of PtdIns and PtdInsP(2) in the mouse.PloS oneb69f958d0a381f3030849518250d3521Anderson KE2013-01-011932-62035463715eed0fc3.36320995DD358A9F-20BB-46DC-B15F-00088FD0825CQuantitation of class IA PI3Ks in mice reveals p110-free-p85s and isoform-selective subunit associations and recruitment to receptorsProceedings of the National Academy of Sciences2c1b247a4f72aa031fc0966736f27c27Tsolakos N2018-01-010027-84245c470237728630.88665696536A3056-A2F4-448D-A71A-1131314CAA2FGß? is a direct regulator of endogenous p101/p110? and p84/p110? PI3K? complexes in mouse neutrophils.Science signalingae569daa6ccaf41d0010a7fe61afd363Rynkiewicz NK2020-01-011945-08775fb8f7363be6f0.582439586CFC2F9D-2675-48EE-A0A6-C20EF795E407Inactivation of the Class II PI3K-C2ß Potentiates Insulin Signaling and Sensitivity.Cell reports83ed02408872fd806a388ba2a32fb91cAlliouachene S2015-01-0156b0cdd8ca10e9.80705522D765ED38-B746-4D9C-ADAB-CF581260E1E5Investigating the effect of arachidonate supplementation on the phosphoinositide content of MCF10a breast epithelial cells.Advances in biological regulationb69f958d0a381f3030849518250d3521Anderson KE2016-01-012212-492656b0cdd9b2d104.68485968BB/I003916/12D9083F0-05FA-4726-9EB2-3FCC293CAAF925Biomolecules & biochemistry999F0B31-F127-410A-A520-963B336BECE725Cell biology29F3DF16-3094-4F79-BC69-8D05FB55182613Omic sciences & technologies945E0A55-10CB-4E91-BCCB-7CB22CFE223212Tools, technologies & methodsEF22E4A7-F12A-4859-8A95-E0179E3570EC12Biological membranes6D0F40FF-D03E-4429-A764-185BC521A84013Catalysis & enzymology2A0F6391-E88A-4396-9D63-25A68EEDA63513Communication & signallingCA10DA58-174F-4FE6-B61B-8EEBFB8192E212Receptors812BD191-6D4F-4F72-852D-0F63AD58ABD812Research approaches937A9F23-021A-4604-8979-A28E0E04F82513Transcriptomics018ACD8A-DE0F-4F6C-82FF-00EFDE30F246Characterising the performance of low loading electrodes for hydrogen technologiesActive2928626StudentshipThis project is associated with deep understanding of the operation and performance of electrodes for electrochemical devices used in electrolysers, flow batteries, and fuel cells. These devices will allow the efficient capture of renewable electricity and storage/interconversion as hydrogen (see Royal Society report &quot;Large-scale electricity storage&quot;). Deployment of electrochemical hydrogen systems is growing at a tremendous pace, but in order to achieve the well defined KPIs we need: a 10-fold reduction in catalyst requirements; significant improvements in performance; and increased longevity. The purpose of this experimental iCASE is the improved performance of these electrodes whilst reducing the catalyst requirements (and thus cost), coupled to improved understanding and ability to model the performance of these systems. Such improvements can only be achieved through a deeper understanding of performance of the electrochemical interface at which reactants, electrons and ions must be efficiently transported to the catalytic interface. These systems are crucial for the UK and world to reach their net-zero aspirations by 2050. The topic cuts across a number of themes in the UKRI including the Energy and decarbonisation theme (Solutions to reach net zero), the Manufacturing the future theme and the Physical Sciences theme. The project is extremely well aligned with the UK's 2022 NMS strategy for which 'the measurement infrastructure needed to support the hydrogen economy as it develops' is a government priority2028-09-29798CB33D-C79E-4578-83F2-72606407192CEPSRC2024-09-30INCOME_ACTUAL029286266CFA1E1F-F25C-4C23-8FE1-C47AE53E333EUnclassified02103DD9-A889-4067-B44E-006A617A8BDFEye in the SkyClosed10037439Collaborative R&DAbstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.2023-01-3112E03F45-B517-4D83-A182-3D142D1A471AInnovate UK2022-07-31INCOME_ACTUAL0100374396CFA1E1F-F25C-4C23-8FE1-C47AE53E333EUnclassified0248EF3F-DD65-4561-BD94-020C64D6F13DVANTAGE - An intelligent payload that enables UAVs to autonomously land on maritime vessels, ultimately addressing Urban Air Mobility requirements.Closed10008315BEIS-Funded ProgrammesAbstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.2023-09-29B51DB087-2804-4E46-8896-F5FC40D5A4FDATI2022-03-31INCOME_ACTUAL210000100083156CFA1E1F-F25C-4C23-8FE1-C47AE53E333EUnclassified02563003-C0B6-42B2-BBC9-01F8D289E65EDevelopment of molecular imprinting with liquid chromatographt-massClosed1677978StudentshipDevelopment of molecular imprinting with liquid chromatography-mass spectrometry HD-SRM for the sensitive and selective detection of proteins2019-09-29198E4A3D-B2DC-45D4-8351-7CCEC4061876BBSRC2015-09-30INCOME_ACTUAL0500CanadaUSDEcuadores_ECHuman Proteome Organisation (HUPO) World Congress 2018 Travel Stipend2018-09-01Human Proteome OrganizationA8768D32-7B84-4DB7-B80E-E134F5A5A4875c829082a9f756.50057518Charity/Non Profit2018-08-31300United KingdomGBPUnited Kingdomen_GBJohn Beynon Travel and Conference Fund2018-09-01British Mass Spectrometry SocietyC04B7197-298E-4C21-800E-2EEA3B8171A15c8291ab41ea67.63563298Charity/Non Profit2018-08-31250United KingdomGBPUnited Kingdomen_GBJohn Beynon Travel and Conference Fund2016-09-01British Mass Spectrometry Society8B4FF2FB-EA19-4238-8DB1-812ADE0495DD5c8292ea5aa071.04432429Charity/Non Profit2016-08-31250United KingdomGBPUnited Kingdomen_GBJohn Beynon Travel and Conference Fund2017-09-01British Mass Spectrometry Society3FCCA429-D4C8-4280-A00E-955FA6A8A0CA5c829276b4fe16.71008967Charity/Non Profit2017-08-3100F85950-8BCD-478C-BA26-8061439BEDD4The measurement of KRAS G12 mutants using multiplexed selected reaction monitoring and ion mobility mass spectrometry.Rapid communications in mass spectrometry : RCMf0b853c384b19d76e8c09068e63f8af3Norman RL2020-01-010951-41985e45186db0b557.4718198016779786CFA1E1F-F25C-4C23-8FE1-C47AE53E333EUnclassified027CDB72-517A-4A0D-AE08-00FDFAE8F76CEstablishing a test methodology for PFAS-free barrier materials for the hydrogen economyClosed10158010Grant for R&DThis project addresses the technical challenges associated with accurately measuring hydrogen permeation in rubber materials used in key industries such as energy and automotive. As the UK government advances ambitious hydrogen production goals and global hydrogen demand continues to rise, the establishment of reliable, standardised testing methodologies for hydrogen permeation is critical to ensuring safety, performance, and long-term durability in hydrogen applications. Currently, rubber components in hydrogen systems are evaluated using testing standards from the oil &amp; gas sector, predominantly suited for high-pressure conditions. However, there remains a substantial gap in measurement methodologies where variations in temperature and pressure significantly affect the behaviour of hydrogen permeation and diffusion. The primary focus of this project is therefore the development of robust and standardised test methods capable of quantifying hydrogen permeation under diverse temperature and pressure gradients. These methods will specifically target existing PFAS-free rubber materials, such as EPDM, HNBR, VMQ, and fluoropolymers developed using non-fluoro surfactant technologies and reinforced with graphene and other 2D nanomaterials. This methodology development will leverage the extensive expertise and experience of The University of Manchester. Additionally, the collaborative framework provided by the A4I program will facilitate industry-wide adoption and impact, aligning closely with the UK's hydrogen strategy and broader decarbonisation objectives. Ultimately, the project aims to deliver a reliable, standardised permeation testing protocol, significantly enhancing material selection processes and contributing to the advancement of sustainable, high-performance rubber solutions in the transition to a low-carbon economy.2026-01-3112E03F45-B517-4D83-A182-3D142D1A471AInnovate UK2025-06-30INCOME_ACTUAL10084101580106CFA1E1F-F25C-4C23-8FE1-C47AE53E333EUnclassified027FCA23-6711-407E-A392-020B8329DDABThe role of commensal organisms as pro-infectious agents in Staphylococcus aureus infection dynamics.ClosedMR/R001111/1Research GrantStaphylococcus aureus is an important human pathogen that causes significant death and disease around the world. The problem is exacerbated by the spread of antibiotic resistant strains such as Methicillin Resistant S. aureus (MRSA). MRSA is generally a hospital-associated infection but recently community acquired MRSA has increased in the apparent absence of antibiotic selection. Thus there is a constant need to develop new control regimes for S. aureus, as this organism has begun to show resistance even to new antibiotics such as linezolid and no vaccine is available. To win this battle we must not only produce new treatments and preventative measures but also to understand the complexities of how S. aureus causes disease and thus how interventions can be best utilised, to reduce the disease burden and to minimise the development and spread of resistance to antibiotics. How do humans get infected with S. aureus? We have made the astonishing discovery that relatively harmless skin organisms and even their component cell walls are able to cause significant exacerbation of S. aureus infection, allowing disease to occur with a hugely reduced infectious dose. The most common route of human infection is via a wound and of course this will occur with whatever material gets in, including S. aureus. We have named the material that can augment S. aureus infection as &quot;pro-infectious agents&quot;. We have opened a window on natural infection and in doing so provide novel avenues for trying to understand disease and how we may prevent it. The current application takes an interdisciplinary approach to understand how infection is initiated, the nature of material and organisms that can act as pro-infectious agents, how infection progresses and how such knowledge may be utilized to prevent and/or cure disease. Our background work and that proposed necessitates an integrated team led by a microbiologist and a clinician with research expertise in innate immunity. This combined expertise, coupled with important partners around the world, provides a proven network to underpin our ambitious proposal. Our work is underpinned by the use of animal models of infection, as this complex process cannot be recapitulated in vitro without understanding of what happens in vivo. We have uniquely developed the vertebrate zebrafish embryo as a model of S. aureus systemic disease. This provides a high-throughput, genetically tractable system where host:pathogen interaction can be observed in a living host. The importance of this model is that it has not only provided important insights into pathogenesis but has also informed our use of a mammalian system thus replacing many mouse experiments directly, reducing the numbers of mammals used and refining our approach. We will use a combination of animal models, together with in vitro analysis to determine the breadth of material that can augment S. aureus infection and the cellular and molecular mechanisms underlying this effect. Infection will then be mapped from initiation to endpoint in order to identify bottlenecks as sites for the development on novel intervention strategies. We will use a variety of approaches including state-of-the-art intravital microscopy in all models to determine the pathway of infection. The application provides an integrated package that will further our understanding of disease mechanisms and how this information may be used to inform clinical practice.S. aureus is able to cause a wide range of diseases, with no vaccine available and antibiotic resistance common. It is therefore crucial to understand how S. aureus infection is initiated and progresses in order to identify novel clinical interventions. We have made the exciting discovery that skin commensal organisms, and even their isolated cell walls, can augment S. aureus infection. We have called the augmenting material &quot;pro-infectious agents&quot; and have shown its addition to result in a massive decrease in the inoculum of S. aureus required to cause serious disease. These findings are extremely pertinent to human infection, as this will always occur from within a polymicrobial milieu. It is important now to capitalize on our initial results to understand the breadth and basis of the augmentation phenomenon and how this might be exploited in the development of new approaches to tackle S. aureus. The project will take an interdisciplinary approach, with global partners, using a combination of in vivo and in vitro analyses. We will investigate the molecular basis of pro-infectious agents in terms of those organisms and components able to augment S. aureus disease, coupled with an elucidation of the host response to pro-infectious agents from the molecular, through cellular to the whole host level. This will be set within a determination of the progression of S. aureus infection to understand how disease is established as a complex interaction between host and pathogen and how this dynamic is altered by the presence of pro-infectious agents. Finally, we will examine how our discovery of pro-infectious agents can be utilized in the design of a vaccine and other immunological interventions. Such ambitious goals, from fundamental understanding to translation, can only be achieved via an integrated team of investigators and partners, providing molecular understanding of host:pathogen interaction with important ramifications for human disease.The proposed project will develop an integrated, interdisciplinary platform to study S. aureus disease and the effect of prophylactic and treatment regimes. There will be a variety of impacts over a range of timescales and arenas. - Academia (expected timescale year 1 onwards): will benefit from the information gained during the project, communicated both orally and via publication. Also given the unique use of the zebrafish embryo model of S. aureus pathogenesis, Sheffield will become a hub for collaboration and training. Transgenic zebrafish lines generated in this project will have diverse utility for other applications. The innovative multidisciplinary approaches we are taking will be a model for others working on related problems. - Industry (expected timescale year 2 onwards): will benefit as potential users of the data concerning the use of antibiotics and the establishment of pathogen population dynamics during infection to test existing or develop new control regimes, such as vaccination. Direct links to Biotech companies such as Absynth and pharmaceutical companies such as GSK are in place to facilitate direct translation of key findings. - National Health Service (expected timescale year 2 onwards): The cost to the NHS of healthcare associated infections (of which MRSA is the most significant) has been estimated by the HPA to be &pound;1billion a year in England alone. This is predominantly in terms of delayed discharge, with associated knock-on effects of full hospitals, cancelled operations and lack of beds for non-emergency admissions. If we were able to identify new ways to treat or prevent MRSA, this would have a major impact on the NHS. - Government bodies (expected timescale year 2 onwards): will benefit from the information on the role of microflora in disease augmentation as it will inform decisions as to decontamination of wound sites. - Local communities (expected timescale year 2 onwards): via outreach activities will benefit from greater knowledge of this scientific area and engagement with scientific advances made locally. - Society more broadly (expected timescale year 2 onwards): will benefit if the findings of this project translate to a reduction in the spread of antibiotic resistant bacteria, or new strategies for the treatment of infectious disease. - UK PLC (expected timescale year 2 onwards): will benefit through the development of commercial activities, and added value to both biotechnology and pharmaceutical companies. In addition, the economic burden of MRSA infection in the UK can be counted in the billions of pounds, from missed work and additional costs following hospital discharge. Small reductions in spread of antibiotic resistance could have large impacts on the productivity of the UK workforce. - Undergraduate/postgraduate/post docs (expected timescale year 1 onwards): will benefit through development of skills in cell biology, microscopy, in vivo studies etc. cascaded down through teaching interactions within the lab. - Media (expected timescale year 1 onwards): will benefit through the applicants' participation in radio and newspapers interviews.2021-06-29C008C651-F5B0-4859-A334-5F574AB6B57CMRC2018-01-01INCOME_ACTUAL703187University of CalgaryCanadaThe Calgary Stroke ProgramCalgary Partnership951A47A3-2683-494D-BC73-74F05CFF6DA4Boldock, E., Surewaard, B.G.J., Shamarina, D., Na, M., Fei, Y., Ali, A., Williams, A., Pollitt, E.J.G., Szkuta, P., Morris, P., Prajsnar, T.K., McCoy, K.D., Jin, T., Dockrell, D.H., van Strijp, J.A.G., Kubes, P., Renshaw, S.A. &amp; Foster, S.J. (2018) Human skin commensals augment Staphylococcus aureus pathogenesis. Nature Microbiology doi: 10.1038/s41564-018-0198-3.5c618136179588.97817984-1Testing of novel approaches to understand bacterial pathogenesis. In particular, the use of intravital microscopyWe developed the background data and hypothesesAcademic/University2016-01-01Cheltenham Science Festival 2018Participation in an activity, workshop or similarNational5F6FC8A3-E49A-49BC-97FC-55DAEB9F1112Hands on exhibit and stand in the area of microbiology, microscopy and antimicrobial resistance5c61827fc48641.99105787falseSchools2018Macroautophagy/autophagy functions to degrade cellular components and intracellular pathogens. Autophagy receptors, including SQSTM1/p62, target intracellular pathogens. Staphylococcus aureus is a significant pathogen of humans, especially in immunocompromise. S. aureus may use neutrophils as a proliferative niche, but their intracellular fate following phagocytosis has not been analyzed in vivo. In vitro, SQSTM1 can colocalize with intracellular Staphylococcus aureus, but whether SQSTM1 is beneficial or detrimental in host defense against S. aureus in vivo is unknown. Here we determine the fate and location of S. aureus within neutrophils throughout zebrafish infection. We show Lc3 and Sqstm1 recruitment to phagocytosed S. aureus is altered depending on the bacterial location within the neutrophil and that Lc3 marking of bacterial phagosomes within neutrophils may precede bacterial degradation. Finally, we show Sqstm1 is important for controlling cytosolic bacteria, demonstrating for the first time a key role of Sqstm1 in autophagic control of S. aureus in neutrophils. AR: autophagy receptor; CFU: colony-forming unit; CHT: caudal hematopoietic tissue; GFP: green fluorescent protein; hpf: hours post-fertilization; hpi: hours post-infection; LWT: london wild-type: lyz: lysozyme; Map1lc3/Lc3: microtubule-associated protein 1 light chain 3; RFP: red fluorescent protein; Sqstm1/p62: sequestosome 1; Tg: transgenic; TSA: tyramide signal amplification; UBD: ubiquitin binding domain.BFA5EAC8-FCE0-461F-B48E-7B62447DE19665df9f8c939100.44019563trueNeutrophils use selective autophagy receptor Sqstm1/p62 to target <i>Staphylococcus aureus</i> for degradation <i>in vivo</i> in zebrafishDatabase/Collection of datahttps://tandf.figshare.com/articles/dataset/Neutrophils_use_selective_autophagy_receptor_Sqstm1_p62_to_target_i_Staphylococcus_aureus_i_for_degradation_i_in_vivo_i_in_zebrafish/128513602020This is the raw data supporting the findings (both main text and supplementary) for our manuscript &quot;Clonal population expansion of Staphylococcus aureus during infection can occur due to escape from a finite number of intraphagocyte niches&quot;. Each excel file contains the raw data for each figure.AFF37E7D-E7CB-4265-B162-5CEA8FC450DF63ed5140093da4.56260141trueClonal population expansion of Staphylococcus aureus during infection can occur due to escape from a finite number of intraphagocyte nichesDatabase/Collection of datahttps://figshare.shef.ac.uk/articles/dataset/Clonal_population_expansion_of_Staphylococcus_aureus_during_infection_can_occur_due_to_escape_from_a_finite_number_of_intraphagocyte_niches/185510812023This is the raw data supporting the findings (both main text and supplementary) for our manuscript &quot;Clonal population expansion of Staphylococcus aureus during infection can occur due to escape from a finite number of intraphagocyte niches&quot;. Each excel file contains the raw data for each figure.614DFE9B-3AA4-47B6-A7FC-1D43605E6F3C65f4c13803ad76.53378984trueClonal population expansion of Staphylococcus aureus during infection can occur due to escape from a finite number of intraphagocyte nichesDatabase/Collection of datahttps://figshare.shef.ac.uk/articles/dataset/Clonal_population_expansion_of_Staphylococcus_aureus_during_infection_can_occur_due_to_escape_from_a_finite_number_of_intraphagocyte_niches/18551081/12023Macroautophagy/autophagy functions to degrade cellular components and intracellular pathogens. Autophagy receptors, including SQSTM1/p62, target intracellular pathogens. Staphylococcus aureus is a significant pathogen of humans, especially in immunocompromise. S. aureus may use neutrophils as a proliferative niche, but their intracellular fate following phagocytosis has not been analyzed in vivo. In vitro, SQSTM1 can colocalize with intracellular Staphylococcus aureus, but whether SQSTM1 is beneficial or detrimental in host defense against S. aureus in vivo is unknown. Here we determine the fate and location of S. aureus within neutrophils throughout zebrafish infection. We show Lc3 and Sqstm1 recruitment to phagocytosed S. aureus is altered depending on the bacterial location within the neutrophil and that Lc3 marking of bacterial phagosomes within neutrophils may precede bacterial degradation. Finally, we show Sqstm1 is important for controlling cytosolic bacteria, demonstrating for the first time a key role of Sqstm1 in autophagic control of S. aureus in neutrophils. AR: autophagy receptor; CFU: colony-forming unit; CHT: caudal hematopoietic tissue; GFP: green fluorescent protein; hpf: hours post-fertilization; hpi: hours post-infection; LWT: london wild-type: lyz: lysozyme; Map1lc3/Lc3: microtubule-associated protein 1 light chain 3; RFP: red fluorescent protein; Sqstm1/p62: sequestosome 1; Tg: transgenic; TSA: tyramide signal amplification; UBD: ubiquitin binding domain.A81AA9E1-CE02-4C79-A477-25BEDDDF334B65df9f8d414d01.60695457trueNeutrophils use selective autophagy receptor Sqstm1/p62 to target <i>Staphylococcus aureus</i> for degradation <i>in vivo</i> in zebrafishDatabase/Collection of datahttps://tandf.figshare.com/articles/dataset/Neutrophils_use_selective_autophagy_receptor_Sqstm1_p62_to_target_i_Staphylococcus_aureus_i_for_degradation_i_in_vivo_i_in_zebrafish/12851360/12020This is the raw data supporting the findings (both main text and supplementary) for our manuscript &quot;Staphylococcus aureus cell wall structure and dynamics during host-pathogen interaction&quot;. Each excel file contains the raw data for each figure. Murine work was carried out according to UK law in the Animals (Scientific Procedures) Act 1986, under Project License P3BFD6DB9 (Staphylococcus aureus and other pathogens, pathogenesis to therapy, University of Sheffield Review Board).B7520B3D-93D6-4263-9A82-AD7D988590DF61ebee16a0c0c4.40755595trueStaphylococcus aureus cell wall structure and dynamics during host-pathogen interactionDatabase/Collection of datahttps://figshare.shef.ac.uk/articles/dataset/Staphylococcus_aureus_cell_wall_structure_and_dynamics_during_host-pathogen_interaction/137464692021Staphylococcus aureus is a human commensal organism and opportunist pathogen, causing potentially fatal disease. The presence of non-pathogenic microflora or their components, at the point of infection, dramatically increases S. aureus pathogenicity, a process termed augmentation. Augmentation is associated with macrophage interaction but by a hitherto unknown mechanism. Here, we demonstrate a breadth of cross-kingdom microorganisms can augment S. aureus disease and that pathogenesis of Enterococcus faecalis can also be augmented. Co-administration of augmenting material also forms an efficacious vaccine model for S. aureus. In vitro, augmenting material protects S. aureus directly from reactive oxygen species (ROS), which correlates with in vivo studies where augmentation restores full virulence to the ROS-susceptible, attenuated mutant katA ahpC. At the cellular level, augmentation increases bacterial survival within macrophages via amelioration of ROS, leading to proliferation and escape. We have defined the molecular basis for augmentation that represents an important aspect of the initiation of infection. Animal work in the UK was performed in accordance with the Animal (Scientific Procedures) Act 1986. At the University of Sheffield, work was completed under project licences P3BFD6DB9 and PPL 40/3699 for murine work, or P1A4A7A5E for zebrafish work, with ethical approval from the University of Sheffield Local Ethical Review Panel. At Imperial College London, work was conducted under licence P4C824899 with approval from the Imperial ethical review board. At INRAE, animal work was approved by the local ethics committee (COMETHEA or &quot;Comit&eacute; d'Ethique en Exp&eacute;rimentation Animale&quot;, Centre de Recherche Ile de France - Jouy en Josas - Antony) under the registration numbers 15_08, and by the French Ministry of Higher Education and Research APAFIS #480-2015041518048149v1, where all animal experiments were performed in accordance with European directive 2010/63/EU. Animal experiments in Calgary were approved by the University of Calgary Animal Care Committee and were in compliance with the Canadian Council for Animal Care Guidelines (protocol nr. AC16-0148). MDMs were derived, with informed consent, from the blood of healthy volunteers, in accordance with guidelines from the South Sheffield Research Ethics Committee (07/Q2305/7).2BAA20E5-AF35-43D4-B22E-470336720DF065dfa4072f7e27.63532206trueCommensal bacteria augment Staphylococcus aureus infection by inactivation of phagocyte-derived reactive oxygen speciesDatabase/Collection of datahttps://figshare.shef.ac.uk/articles/dataset/Commensal_bacteria_augment_Staphylococcus_aureus_infection_by_inactivation_of_phagocyte-derived_reactive_oxygen_species/151347092021Staphylococcus aureus is a human commensal organism and opportunist pathogen, causing potentially fatal disease. The presence of non-pathogenic microflora or their components, at the point of infection, dramatically increases S. aureus pathogenicity, a process termed augmentation. Augmentation is associated with macrophage interaction but by a hitherto unknown mechanism. Here, we demonstrate a breadth of cross-kingdom microorganisms can augment S. aureus disease and that pathogenesis of Enterococcus faecalis can also be augmented. Co-administration of augmenting material also forms an efficacious vaccine model for S. aureus. In vitro, augmenting material protects S. aureus directly from reactive oxygen species (ROS), which correlates with in vivo studies where augmentation restores full virulence to the ROS-susceptible, attenuated mutant katA ahpC. At the cellular level, augmentation increases bacterial survival within macrophages via amelioration of ROS, leading to proliferation and escape. We have defined the molecular basis for augmentation that represents an important aspect of the initiation of infection. Animal work in the UK was performed in accordance with the Animal (Scientific Procedures) Act 1986. At the University of Sheffield, work was completed under project licences P3BFD6DB9 and PPL 40/3699 for murine work, or P1A4A7A5E for zebrafish work, with ethical approval from the University of Sheffield Local Ethical Review Panel. At Imperial College London, work was conducted under licence P4C824899 with approval from the Imperial ethical review board. At INRAE, animal work was approved by the local ethics committee (COMETHEA or &quot;Comit&eacute; d'Ethique en Exp&eacute;rimentation Animale&quot;, Centre de Recherche Ile de France - Jouy en Josas - Antony) under the registration numbers 15_08, and by the French Ministry of Higher Education and Research APAFIS #480-2015041518048149v1, where all animal experiments were performed in accordance with European directive 2010/63/EU. Animal experiments in Calgary were approved by the University of Calgary Animal Care Committee and were in compliance with the Canadian Council for Animal Care Guidelines (protocol nr. AC16-0148). MDMs were derived, with informed consent, from the blood of healthy volunteers, in accordance with guidelines from the South Sheffield Research Ethics Committee (07/Q2305/7).A0C68E72-4CA8-4615-AACE-36A402C7586965dfa43a09d050.62768875trueCommensal bacteria augment Staphylococcus aureus infection by inactivation of phagocyte-derived reactive oxygen speciesDatabase/Collection of datahttps://figshare.shef.ac.uk/articles/dataset/Commensal_bacteria_augment_Staphylococcus_aureus_infection_by_inactivation_of_phagocyte-derived_reactive_oxygen_species/15134709/120218EB8B9BB-3FCC-409D-A1F0-B0CB73F2DE6DCommensal bacteria augment Staphylococcus aureus infection by inactivation of phagocyte-derived reactive oxygen species.PLoS pathogense8b8b70d41204ef67f5afc08219a57f4Gibson JF2021-01-011553-736661f97c61515d71.4303464380011005-12C1-4B26-AF59-79FF536C2B75Resident risks.Nature microbiology5e2d45794933216b91d672e9e397b613Spoto M2018-01-012058-5276675243265a45a3.47669118C61993C8-B01C-4F4C-977A-C324DDDE8796Human skin commensals augment Staphylococcus aureus pathogenesis.Nature microbiologya489d56a05ef53914f775cf3ad41cfc6Boldock E2018-01-012058-52765b9bc2dfed7633.785414061F784640-4DD6-4629-AE59-A174B50B048DTetracycline and Oxacillin Act Synergistically on Biofilms and Display Increased Efficacy In Vivo Against Staphylococcus aureus.Current microbiology7a94b2ff9076a8fa3e596e8da25091dbTooke AK2024-01-010343-865167400206739663.405532899C7249F4-DF0A-4897-9C8C-17D3BC9B65B7Neutrophils use selective autophagy receptor p62/SQSTM1 to target Staphylococcus aureus for degradation in vivo in zebrafish6d0058b9873c2884fe0fb044f03c6b70Gibson J2019-01-0165bb8296101831.56601605E9605716-E9F5-43C6-B1DA-034634AB2C82Demonstration of the role of cell wall homeostasis in Staphylococcus aureus growth and the action of bactericidal antibiotics.Proceedings of the National Academy of Sciences of the United States of America9289d41c2d59bb76d0611b6cfef2d9d0Salamaga B2021-01-010027-842461fc6f31da42b2.99125838303BE387-4437-4081-94CF-7635F84CEA76The Role of Macrophages in Staphylococcus aureus Infection.Frontiers in immunologye5e5a2af2acfd4bf9bff68ccf2d49f88Pidwill GR2020-01-011664-32246014f227b3f057.909928448095AC56-2248-4981-9234-AB24CF1A0261Staphylococcus aureus cell wall structure and dynamics during host-pathogen interaction.PLoS pathogens71f47816609b3b09ea897307e94ac64fSutton JAF2021-01-011553-736661f7056098a6f1.721846650CF6D889-1844-4051-AFF1-87AB3F0A3438Clonal population expansion of Staphylococcus aureus occurs due to escape from a finite number of intraphagocyte niches.Scientific reportse5e5a2af2acfd4bf9bff68ccf2d49f88Pidwill GR2023-01-012045-232263ec2738764249.305770672C2EA4BA-7624-4F17-8114-1C80A229A80BNeutrophils use selective autophagy receptor Sqstm1/p62 to target Staphylococcus aureus for degradation in vivo in zebrafish.Autophagye8b8b70d41204ef67f5afc08219a57f4Gibson JF2021-01-011554-86275f8a0df426a5f2.03078697ED309948-0B9A-47A2-A415-A752F51912E92601. Identification of Staphylococcus aureus Genetic Factors Associatiated with the Subversion of Macrophage Phagosomal AcidificationOpen Forum Infectious Diseases9819be6ab36d0e3a67623b97cea4fd62Morris P2019-01-0167ab6c79159f02.25034675MR/R001111/16CFA1E1F-F25C-4C23-8FE1-C47AE53E333EUnclassified02D113B2-C9DC-486E-953C-016B03A07CB9Evaluation and Demonstration of Gravity GradiometersClosedEP/R020353/1Research GrantQinetiQ Ltd, Imperial College and the University of Oxford will jointly investigate the use of novel gravity gradiometers to detect buried objects such as pipes, tunnels and sinkholes. We will model the gravitational field of a range of buried targets, and investigate methods to mitigate noise and clutter. We will determine what type of objects are detectable, at what range, and develop some outline Concepts of Operation. We will investigate the applicability of a range of sensors, both high performance &quot;cold atom fountains&quot; and lower-cost MEMS-based devices, configured as gravity gradiometers, and compare them to the performance of commercially available sensors. We will build a single-axis gravity gradiometer based on two existing gravimeters, and use this to validate our models with through short field trials to demonstrate the detection of a buried object.Economic benefits: Four million holes are dug in British roads every year, 300,000 of them in London alone. 36% of London traffic delays caused by roadworks, with a total cost to London business is not far short of &pound;1 billion. This is likely to be replicated in cities across the country. According to some estimates, half the holes dug by the water industry are in the wrong place. The ability to sense a buried pipe before starting to dig would greatly reduce unnecessary disruption. A major benefit of this research will be to inform the National Quantum Programme of expected targets and detection ranges using gravity gradiometers based on the published performance of a range of gravity sensors, including ones being developed by the National Quantum Programme. This information is likely to help guide the parties involved in the development of their devices. Social impacts: The UK does not have a sovereign capability in full-tensor gravity gradiometry. The classical-technology Lockheed Martin FTG instrument is ITAR-controlled, and can only be used for a very strictly controlled set of applications. This work would pave the way towards a sovereign capability which could be used for both commercial and military applications without ITAR restrictions. Environmental impacts: Stand-off detection of buried objects will have a positive environmental impact, as it will allow remote sensing as opposed to physical investigation and inspection. Earth and planetary science: though deployment of gradiometers in Earth orbit is being pursued separately with Thales-Alenia UK, some of the technical challenges will require similar approaches. We would therefore anticipate cross benefits from this project to Earth and planetary gravity measurements using compact gradiometers. Such measurements aim to quantify on Earth the effects of climate change through ice-sheet loss and aquifer degradation. Regional impacts: Remote detection of dense smuggled material at borders/ports would potentially not only increase security but also reduce delays, supporting the freer movement of goods.2019-02-28798CB33D-C79E-4578-83F2-72606407192CEPSRC2017-11-01INCOME_ACTUAL40242Imperial College LondonUnited KingdomDepartment of ComputingDevelopment of Terrestrial Gradiometer8EE026E1-0AE2-4CE4-AB2B-567E9117594CProposals still in preparation58c52f6fe00229.64575348-1Development of MEMs device (Imperial) Development of Gradiometer System (Qintetiq)Development of gradiometer based upon Imperial MEMs device Development of support electronicsAcademic/University2017-01-01QinetiqUnited KingdomDevelopment of Terrestrial GradiometerBE0F78E2-724A-49F1-9066-380A9BACBBB9Proposals still in preparation58c52f6fe00229.64575348-2Development of MEMs device (Imperial) Development of Gradiometer System (Qintetiq)Development of gradiometer based upon Imperial MEMs device Development of support electronicsPrivate2017-01-0127537628United KingdomGBPUnited Kingdomen_GBUK National Quantum Technology Hub in Sensing and Timing2024-11-02Engineering and Physical Sciences Research Council (EPSRC)EP/T001046/134F9F3DE-9CA5-4A35-8B94-310872D4228965eefed3d4a838.70323518Public2019-12-01612F4AE6-A36E-45CF-AA29-AFA192EB03D8Standing on Apollo's Shoulders: A Microseismometer for the MoonThe Planetary Science Journal0e5a2727438c34889f549446115c5ae3Nunn C2021-01-016409c4a647cec8.00525017EP/R020353/16CFA1E1F-F25C-4C23-8FE1-C47AE53E333EUnclassified