Tissue and organ development in the intact animal - Agriculture, Animal Welfare, Developmental Biology, Diet and Health , Genetically Modified Organisms
Lead Research Organisation:
University of Edinburgh
Department Name: The Roslin Institute
Abstract
Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.
Technical Summary
We aim investigate whole animal biology through mechanistic studies. Using the skills and capabilities at The Roslin Institute we are ideally placed to study cellular differentiation at the tissue level in vivo – across a range of species including rodent, poultry, livestock and companion animals. Furthermore, we aim to identify general mechanisms that function across a range of species thus exploiting comparative biology to identify over-arching principles and rate-limiting processes. We aim to compared and contrast cellular differentiation in the foetus or embryo with that in the adult animal and will exploit natural and engineered mutants to achieve these goals. The objectives of the theme are: To define endocrine regulation of ovarian follicular recruitment. To define role of macrophages in embryonic development. To identify centrosome/cilia signalling functions during vertebrate development. To expand application of transgenic technologies in the study of development. To characterise regulation of bone mineralization, growth and differentiation. To assess how early life experiences affect fundamental patterns of organ development and function in the foetus and neonate.
Planned Impact
unavailable
Organisations
People |
ORCID iD |
| Christopher Whitelaw (Principal Investigator) |
Publications
Arends MJ
(2016)
Animal and cellular models of human disease.
in The Journal of pathology
Boesze Zsuzsanna
(2008)
Producing recombinant human milk proteins in the milk of livestock species
in BIOACTIVE COMPONENTS OF MILK
Breton A
(2013)
Derivation and characterization of induced pluripotent stem cells from equine fibroblasts.
in Stem cells and development
Bruce A
(2013)
Novel GM animal technologies and their governance.
in Transgenic research
Burkard C
(2018)
Pigs Lacking the Scavenger Receptor Cysteine-Rich Domain 5 of CD163 Are Resistant to Porcine Reproductive and Respiratory Syndrome Virus 1 Infection.
in Journal of virology
Bösze Z
(2008)
Producing recombinant human milk proteins in the milk of livestock species.
in Advances in experimental medicine and biology
Capocefalo A
(2009)
p21(Waf1/Cip1) as a molecular sensor for BoHV-4 replication.
in Journal of virological methods
Carlson DF
(2012)
Efficient TALEN-mediated gene knockout in livestock.
in Proceedings of the National Academy of Sciences of the United States of America
Chang SP
(2013)
Relative transgene expression frequencies in homozygous versus hemizygous transgenic mice.
in Transgenic research
Clark EL
(2017)
A high resolution atlas of gene expression in the domestic sheep (Ovis aries).
in PLoS genetics
Fahrenkrug SC
(2010)
Precision genetics for complex objectives in animal agriculture.
in Journal of animal science
Forster R
(2010)
The minipig as a platform for new technologies in toxicology.
in Journal of pharmacological and toxicological methods
Gottardo P
(2019)
A Strategy To Exploit Surrogate Sire Technology in Livestock Breeding Programs
in G3 Genes|Genomes|Genetics
He Z
(2016)
Comparison of surrogate reporter systems for enrichment of cells with mutations induced by genome editors.
in Journal of biotechnology
He Z
(2015)
Highly efficient targeted chromosome deletions using CRISPR/Cas9.
in Biotechnology and bioengineering
Hodgkiss-Geere HM
(2011)
Cardiac specific gene expression changes in long term culture of murine mesenchymal stem cells.
in International journal of stem cells
Hodgkiss-Geere HM
(2012)
Characterisation and cardiac directed differentiation of canine adult cardiac stem cells.
in Veterinary journal (London, England : 1997)
Hodgkiss-Geere HM
(2012)
Characterisation and differentiation potential of bone marrow derived canine mesenchymal stem cells.
in Veterinary journal (London, England : 1997)
Hoyos-Flight M
(2017)
Genome Editing and the Future of Farming meeting report.
in Transgenic research
Huber RC
(2013)
Behaviour of postnatally growth-impaired mice during malnutrition and after partial weight recovery.
in Nutritional neuroscience
Huber RC
(2012)
Welfare assessment in transgenic pigs expressing green fluorescent protein (GFP).
in Transgenic research
HUME D
(2011)
The future of animal production: improving productivity and sustainability
in The Journal of Agricultural Science
Jenko J
(2015)
Potential of promotion of alleles by genome editing to improve quantitative traits in livestock breeding programs.
in Genetics, selection, evolution : GSE
Jiang C
(2014)
Promoter characterization and functional association with placenta of porcine MAGEL2.
in Gene
Kolb AF
(2011)
Milk lacking a-casein leads to permanent reduction in body size in mice.
in PloS one
Kolb AF
(2013)
Mammary gland development is delayed in mice deficient for aminopeptidase N.
in Transgenic research
Kostic C
(2013)
Rapid cohort generation and analysis of disease spectrum of large animal model of cone dystrophy.
in PloS one
Le Provost F
(2010)
Zinc finger nuclease technology heralds a new era in mammalian transgenesis.
in Trends in biotechnology
Lillico S
(2016)
Mammalian interspecies substitution of immune modulatory alleles by genome editing
in Scientific Reports
Lillico SG
(2013)
Live pigs produced from genome edited zygotes.
in Scientific reports
Lu ZH
(2011)
Strain-specific copy number variation in the intelectin locus on the 129 mouse chromosome 1.
in BMC genomics
Manickam R
(2008)
Mammary gland differentiation inversely correlates with GDF-8 expression.
in Molecular reproduction and development
Nelson L
(2008)
An animal model to evaluate the function and regulation of the adaptively evolving stress protein SEP53 in oesophageal bile damage responses.
in Cell stress & chaperones
Palgrave C
(2010)
Dynein light chain 1 (LC8) sequence is highly conserved between pig species
in Animal Genetics
Palgrave CJ
(2011)
Species-specific variation in RELA underlies differences in NF-?B activity: a potential role in African swine fever pathogenesis.
in Journal of virology
Pang L
(2014)
p21 WAF1 is component of a positive feedback loop that maintains the p53 transcriptional program
in Cell Cycle
Pang LY
(2013)
Feline mammary carcinoma stem cells are tumorigenic, radioresistant, chemoresistant and defective in activation of the ATM/p53 DNA damage pathway.
in Veterinary journal (London, England : 1997)
Pridans C
(2014)
Lentiviral vectors containing mouse Csf1r control elements direct macrophage-restricted expression in multiple species of birds and mammals.
in Molecular therapy. Methods & clinical development
Proudfoot C
(2015)
Genome edited sheep and cattle.
in Transgenic research
Ritchie WA
(2009)
Transgenic sheep designed for transplantation studies.
in Molecular reproduction and development
Rowe JM
(2008)
Illuminating role of CYP1A1 in skin function.
in The Journal of investigative dermatology
Scudamore CL
(2016)
Recommendations for minimum information for publication of experimental pathology data: MINPEPA guidelines.
in The Journal of pathology
Sharma R
(2014)
Generation of functional neurons from feeder-free, keratinocyte-derived equine induced pluripotent stem cells.
in Stem cells and development
Strathdee D
(2008)
Distal transgene insertion affects CpG island maintenance during differentiation.
in The Journal of biological chemistry
Tait-Burkard C
(2018)
Livestock 2.0 - genome editing for fitter, healthier, and more productive farmed animals.
in Genome biology
Teboul L
(2017)
Introduction to Mammalian Genome Special Issue: Genome Editing.
in Mammalian genome : official journal of the International Mammalian Genome Society
Tong J
(2011)
Tissue-specific and expression of porcine growth hormone gene in BAC transgenic mice.
in Transgenic research
Vasey DB
(2009)
CMV enhancer-promoter is preferentially active in exocrine cells in vivo.
in Transgenic research
| Description | A strategic approach to identifying and combating porcine reproductive and respiratory syndrome virus outbreaks and other porcine viral diseases |
| Amount | £787,016 (GBP) |
| Funding ID | BB/R013187/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 03/2018 |
| End | 03/2021 |
| Description | General Scheme - Bruce Whitelaw - Editing RELA S531P |
| Amount | £30,000 (GBP) |
| Organisation | Genus plc |
| Sector | Private |
| Country | United Kingdom |
| Start | 03/2017 |
| End | 12/2019 |
| Description | General Scheme - Christine Tait-Burkard - PRRSV study at Moredun to evaluate CD163 edited pigs |
| Amount | £48,812 (GBP) |
| Organisation | Genus plc |
| Sector | Private |
| Country | United Kingdom |
| Start | 03/2017 |
| End | 09/2017 |
| Description | Research Grant - Christine Tait-Burkard - Nanobodies as antiviral PRRSV agents |
| Amount | £59,802 (GBP) |
| Organisation | Eco Animal Health Ltd |
| Sector | Private |
| Country | United Kingdom |
| Start | 03/2018 |
| End | 03/2019 |
| Description | Responsive Mode - Christine Tait-Burkard - Understanding the CD163 - PRRS virus interaction to improve genetic engineering for resistance |
| Amount | £674,353 (GBP) |
| Funding ID | BB/R004463/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 12/2017 |
| End | 11/2020 |