Cryo-electron tomography of CO2-fixing pyrenoids to guide synthetic assembly
Lead Research Organisation:
University of York
Department Name: Biology
Abstract
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| Description | The award was to further interactions between York and Basel and to develop cryoET expertise at York. Several projects have initiated from it. We have recently published work established during this collaboration (Nam et al. 2024 Cell). I have recently submitted a Faraday Discovery Fellowship application with Ben Engel as a core collaborator. We are continuing to collaborate on multiple projects that combine our team's expertise. |
| Exploitation Route | York used outputs from this award to successfully secure an energy filter to enable higher resolution cryo-EM/ET (grant number: BB/Z515668/1). Future publications are expected to initiate new projects in the field of pyrenoid cryoET. |
| Sectors | Education Environment |
| Description | Data from the award resulted in a successful BBSRC-ALERT grant that is enabling enhanced cryo-EM and cryo-ET at York. This is helping accelerate and expand structural biology work at York, establishing a broad expertise and external recognition. |
| First Year Of Impact | 2024 |
| Sector | Pharmaceuticals and Medical Biotechnology,Other |
| Impact Types | Economic |
| Description | An imaging filter to enable high-resolution cryogenic electron tomography (cryo-ET) at York |
| Amount | £680,091 (GBP) |
| Funding ID | BB/Z515668/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 06/2024 |
| End | 07/2025 |
| Title | Cryo-electron tomography |
| Description | Development of cryo-ET analysis pipelines at the University of York |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2024 |
| Provided To Others? | No |
| Impact | Analysis of cryo-ET datasets. Currently working towards publication. |
| Description | Cryo electron tomography with Ben Engel, Basel |
| Organisation | University of Basel |
| Department | Biozentrum Basel |
| Country | Switzerland |
| Sector | Academic/University |
| PI Contribution | We will be providing biological samples for imaging. |
| Collaborator Contribution | They will be providing imaging expertise and facilities. A PhD student, Sabina Musial, has spent ~10 weeks based in the Engel lab learning cryoET data acquisition and analysis. |
| Impact | Further funding. Collaborating on a range of projects. Publications are in the pipeline. |
| Start Year | 2022 |
| Description | Invited Seminar (Biozentrum, Basel University) |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Invited seminar to research groups. |
| Year(s) Of Engagement Activity | 2023 |