High-field NMR Spectroscopy for Biomolecular Research
Lead Research Organisation:
University of Bristol
Department Name: Chemistry
Abstract
NMR spectroscopy is the most information-rich analytical technique available to molecular research, but requires a high level of instrumentation to maximise the benefits of such analyses. In particular, its' application to materials of biological interest requires high quality instrumentation, with powerful superconducting magnets for signal resolution (500MHz or greater) and maximum sensitivity for proton nuclei. The Bristol NMR Facility currently supports a substantial number of researchers in the Biomolecular field and current NMR hardware is no longer sufficient to fulfil the demands of the cutting edge research projects conducted - which covers areas such as biosynthesis, protein structure and dynamics, potential disease treatments (Alzheimers and Cystic Fybrosis), bionanotechnology and membrane protein-ligand interactions. To this end, a modern 500MHz automated NMR spectrometer is required enabling these research fields to take advantage of the latest hardware and experimental developments required to achieve their aims.
Technical Summary
Biomolecular research at Bristol has a high demand for high resolution, high-sensitivity 1H-based NMR spectroscopy which cannot be fulfilled with current hardware. Over 60 biomolecular researchers require routine access to >400MHz spectroscopy in a range of projects spanning biosynthesis, protein structure and dynamics, bionanotechnology, membrane protein-ligand interactions, pharmaceutical development and ion transport in cell membranes. A 1H-sensitive, triple resonance 500MHz automated spectrometer will allow these research workers routine access to modern experimental methods (HSQC, field-gradient NOE, etc) at sufficiently high resolution for their needs. The rapid access to molecular structure elucidation, dynamics measurements and binding studies will allow more efficient and effective research programmes to be progressed and new projects to be developed at Bristol.
Publications
Butts CP
(2011)
High precision NOEs as a probe for low level conformers--a second conformation of strychnine.
in Chemical communications (Cambridge, England)
Jones CR
(2011)
Accuracy in determining interproton distances using Nuclear Overhauser Effect data from a flexible molecule.
in Beilstein journal of organic chemistry
Burns M
(2014)
Assembly-line synthesis of organic molecules with tailored shapes.
in Nature
Ackrill TD
(2015)
Synthesis of Diarylheptanoid Scaffolds Inspired by Calyxins I and J.
in Organic letters
Ndukwe I
(2015)
Pure-shift IMPRESS EXSIDE - Easy measurement of 1 H- 13 C scalar coupling constants with increased sensitivity and resolution
in RSC Advances
Ndukwe I
(2016)
EXtended ACquisition Time (EXACT) NMR-A Case for 'Burst' Non-Uniform Sampling
in ChemPhysChem
Carter TS
(2016)
Platform Synthetic Lectins for Divalent Carbohydrate Recognition in Water.
in Angewandte Chemie (International ed. in English)
Mooibroek TJ
(2016)
Synthesis and evaluation of a desymmetrised synthetic lectin: an approach to carbohydrate receptors with improved versatility.
in Organic & biomolecular chemistry
Mora NL
(2016)
Targeted anion transporter delivery by coiled-coil driven membrane fusion.
in Chemical science
RĂos P
(2017)
Enantioselective carbohydrate recognition by synthetic lectins in water
in Chemical Science
Singleton WG
(2017)
Convection enhanced delivery of panobinostat (LBH589)-loaded pluronic nano-micelles prolongs survival in the F98 rat glioma model.
in International journal of nanomedicine
Alder RW
(2017)
Perhydrohelicenes and other diamond-lattice based hydrocarbons: the choreography of inversion.
in Chemical science
Silvi M
(2018)
Enantiospecific Three-Component Alkylation of Furan and Indole
in Chemistry - A European Journal
Costil R
(2018)
a-Methyl phenylglycines by asymmetric a-arylation of alanine and their effect on the conformational preference of helical Aib foldamers.
in Organic & biomolecular chemistry
Dias CM
(2018)
Anthracene Bisureas as Powerful and Accessible Anion Carriers.
in Chemistry (Weinheim an der Bergstrasse, Germany)
Farndon JJ
(2018)
Stereospecific Alkene Aziridination Using a Bifunctional Amino-Reagent: An Aza-Prilezhaev Reaction.
in Journal of the American Chemical Society
Nofiani R
(2018)
Strobilurin biosynthesis in Basidiomycete fungi.
in Nature communications
Mas-RosellĂł J
(2018)
Enantioselectively functionalised phenytoin derivatives by auxiliary-directed N to C aryl migration in lithiated a-amino nitriles.
in Chemical communications (Cambridge, England)
Niwetmarin W
(2018)
(-)-Cytisine: Access to a stereochemically defined and functionally flexible piperidine scaffold.
in Organic & biomolecular chemistry
De Mattos-Shipley KMJ
(2018)
The cycloaspeptides: uncovering a new model for methylated nonribosomal peptide biosynthesis.
in Chemical science
Ganesh V
(2018)
Chiral Aniline Synthesis via Stereospecific C(sp3)-C(sp2) Coupling of Boronic Esters with Aryl Hydrazines.
in Organic letters
Shu C
(2018)
Synthesis of Functionalized Cyclopropanes from Carboxylic Acids by a Radical Addition-Polar Cyclization Cascade.
in Angewandte Chemie (International ed. in English)
Adam C
(2018)
The Role of Terminal Functionality in the Membrane and Antibacterial Activity of Peptaibol-Mimetic Aib Foldamers.
in Chemistry (Weinheim an der Bergstrasse, Germany)
Dalling AG
(2018)
Synthesis of Nitrogen Heterocycles via Directed Carbonylative C-C Bond Activation of Cyclopropanes.
in Chimia