Understanding factors that influence ionisable xenobiotic bioaccumulation using a Fish in vitro Gill Cell culture System (FIGCS)
Lead Research Organisation:
King's College London
Department Name: Diabetes & Nutritional Sciences
Abstract
The aims of the project are:
1. To measure the PH of the Fish Gill Cell culture system (FIGCS) boundary layer, a factor that influences ionisable compound uptake.
2. To characterize and localize potential pharmaceutical and personal care products (PPCP) transport proteins on the gill.
3. Assess the uptake and excretion of ionisable PPCPs accross FIGCS in differing water chemistries and develop uptake and/or accumulation models.
1. To measure the PH of the Fish Gill Cell culture system (FIGCS) boundary layer, a factor that influences ionisable compound uptake.
2. To characterize and localize potential pharmaceutical and personal care products (PPCP) transport proteins on the gill.
3. Assess the uptake and excretion of ionisable PPCPs accross FIGCS in differing water chemistries and develop uptake and/or accumulation models.
People |
ORCID iD |
| Nicolas Bury (Primary Supervisor) | |
| Elisabeth Chang (Student) |
Studentship Projects
| Project Reference | Relationship | Related To | Start | End | Student Name |
|---|---|---|---|---|---|
| BB/M014827/1 | 01/10/2015 | 30/09/2019 | |||
| 1670838 | Studentship | BB/M014827/1 | 01/10/2015 | 30/09/2019 | Elisabeth Chang |
| Description | A gill model was used to investigate the movement of pharmaceuticals between water and fish. It was determined that although pH and speciation of the pharmaceuticals had a substantial effect on permeability, the greatest fluctuations were not necessarily around the pKa. |
| Exploitation Route | This discovery is important in the context of predicting environmental risk of pharmaceuticals to the aquatic environment. |
| Sectors | Pharmaceuticals and Medical Biotechnology |
| Description | Faculty of Life Sciences and Medicine Travel Bursary. |
| Amount | £250 (GBP) |
| Organisation | King's College London |
| Sector | Academic/University |
| Country | United Kingdom |
| Start | 06/2017 |
| End | 06/2017 |
| Description | SEB Travel Grant |
| Amount | £90 (GBP) |
| Organisation | Society for Experimental Biology (SEB) |
| Sector | Academic/University |
| Country | Global |
| Start | 05/2017 |
| End | 05/2017 |
| Title | HPLC Method with FIGCS |
| Description | An HPLC method was developed to detect and quantify pharmaceuticals from the apical compartment matrix incubated with primary gill cells, used as a proxy to measure uptake into the fish in vitro gill cell culture system. |
| Type Of Material | Biological samples |
| Year Produced | 2019 |
| Provided To Others? | Yes |
| Impact | Method was used to measure the uptake of various pharmaceuticals in the fish in vitro gill cell culture system and the results were analysed by multivariate analysis to determine what molecular descriptors influenced uptake. |
| URL | https://pubs.acs.org/doi/10.1021/acs.est.8b04394 |
| Description | Development of pH sensitive extracellular probe |
| Organisation | Queen Mary University of London |
| Department | School of Biological and Chemical Science QMUL |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Researched the type of extracellular probe that would be compatible with the in vitro gill cell culture system, including the insertion portion and the pH sensitive portion. |
| Collaborator Contribution | Created the ratiometric extracellular pH probe to be used with the in vitro gill cell culture system. |
| Impact | The outcome of the collaboration was the creation of a ratiometric pH sensitve probe to be used with the in vitro gill cell culture system to measure the extracellular pH directly at the membrane. The disciplines involved were chemistry, microscopy and biology. |
| Start Year | 2018 |
| Description | BBSRC Skills School: Broadening Horizons: Cultivating an Innovative Mind Set |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Postgraduate students |
| Results and Impact | BBSRC funded students attended a two day skills school in collaboration with industry that included visiting the facilities, sparking meaningful discourse regarding career opportunities between the students and industry representatives. |
| Year(s) Of Engagement Activity | 2018 |
| Description | Conference Presentation at SEB Annual Meeting |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Other audiences |
| Results and Impact | Gave a talk at the Society of Experimental Biology Annual Meeting in Gothenburg titled: Use of an in vitro fish gill model to better understand factors that influence pharmaceutical uptake, which was followed by good discussion afterwards with other scientists and increased interest in the research. |
| Year(s) Of Engagement Activity | 2017 |
| Description | Poster at SEB: Improving Experimental Approaches in Animal Biology: Implementing the 3Rs |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Other audiences |
| Results and Impact | Poster presentation at SEB Improving Experimental Approaches in Animal Biology: Implementing the 3Rs titled: Using fish in vitro models to characterize uptake and efflux of pharmaceuticals and PPCPs. |
| Year(s) Of Engagement Activity | 2016 |
| Description | Poster at SETAC Europe 27th Annual Meeting |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Other audiences |
| Results and Impact | Poster presentation at SETAC Europe 27th Annual Meeting titled: Using in vitro gill cell culture models for determining factors influencing freshwater pharmaceutical uptake, engaged with many conference attendees in discussion of research and gill cell culture model. |
| Year(s) Of Engagement Activity | 2017 |