High-throughput analysis of protein-RNA interactionsHigh-throughput analysis of protein-RNA interactions using Scaffold Independent Analysis and Principal Component AnalysisTechnology assay or reagentThis is a new tool. In the group has allowed to analyze a large number of protein-RNA binding domainsfalse2D NMR lineshape analysisA tool to extract kinetics and affinity information from 2D NMR lineshape analysis during a titration. This work is a collaboration with John Christodoulou's group.Technology assay or reagentMore than 100 users have visited the site.true2016Isolation of stromal nuclei from fetal tissue samplesIn the age of single-cell RNA sequencing, several tissues remain under studied due to technical challenges associated with cell-dissociation and extraction. One such tissue, which is of interest in my fellowship project are bone and joint tissues, for which the cell types are embedded in dense collagen matrices and in calcified bone. Through optimisation of existing protocols to isolate single-nuclei suspensions, we have developed a way to isolate fetal synovial joint bone nuclei, and produced good quality datasets using single-cell technologies.Biological samplesWe will publish this methodology in a manuscript in an open access journal once the body of related work is completed.false2021Flight planning protocol for DJImini4 droneWe optimised a protocol to be able to perform drone flight planning with a model that doesn't offer it. Despite other models do have this feature integrated, the DJImini4 does not allow to plan flights in advance ensuring enough overlapping between images. We developed a pipeline to use this feature by building a flight plan online and importing it to the RCI so it can be used.Improvements to research infrastructureThis protocol allowed us to perform multiple flights that can be easily repeated next year to perform the vegetation surveys needed for the project. Flight planning ensures good quality images and battery efficient use.false2024Hyperspectral camera for invasive plant stress monitoringWe developed the pipeline to use a hyperspectral camera to obtain several vegetation indexes of invasive plants leaves to monitor their stress responses.Improvements to research infrastructureThe protocol is being used to evaluate the stress responses of invasive plants at their range edges.false2024BMS SLE Conceptual Model Steering Committee - 10/4/2018I am involved with BMS in developing an SLE Conceptual Model for assessing the health economics of SLE. Membership of this Steering Committee has allowed me to contribute to this work and new methodology.Model of mechanisms or symptoms - humanWe have set up a initial model and are now applying for research support to develop and refine the modellingfalse2018Lupus Foundation of America Disease Assessment Training (LFA POINT)We have licensed in the LFA POINT training system to train our SLE researchers across the UK to get training in disease assessment tools. This is the first time a non-commercial group has used this training tool and it will enhanced our ability to do SLE trials and studies in the UKPhysiological assessment or outcome measureWill report uptake and outcomes latertrue2016Transgenic DrosophilaDrosophila stocks expressing mutant versions of pacman and Dis3L2. These have been made available upon request or sent to the Bloomington stock centre for distribution upon request.Model of mechanisms or symptoms - non-mammalian in vivoAcknowledgements on publicationstrue2013Antibody to Pacman proteinWe have generated an excellent antibody to the Drosophila Pacman protein which best antibody available worldwide.Biological samplesAcknowledgements on publicationstruexTMS: Flexible Transcranial Magnetic Stimulation SystemThe xTMS system is a programmble transcranial magnetic stimulator device. The xTMS system enables more control on the electric field pulse shape than conventional TMS device. The pTMS2 system enables PWM electric field pulse with control over the pulse shape, duration, directionality, pulse type such as mono-phasic, bi-phasic and polyphasic. It can be used in single pulse mode or repetitive TMS mode. The xTMS system requires a stimulation coil to deliver the magnetic stimulation. Brief stimuli of an electromagnetic field are applied to the neural tissues with current-carrying treatment coils. The fast-changing field induces electrical currents within the neurons and modulates cell activity. Using the xTMS device, it is possible to change the stimulus waveform and polarity of each pulse in the pulse train. It is intended for use by, or under the supervision of, a medical practitioner or investigator who has knowledge about the principles of TMS, physiology and potential side effects of TMS. It's the user's responsibility to design and validate safeness of their stimulation protocols.Improvements to research infrastructureWe have completed the first clinical study using the xTMS approach, and have now initiated a second study at Oxford. For this year, we are expanding to collaborations at King's College, London and Cambridge University. The studies are in publication review. Note that this project includes a collaboration with Magstim Ltd in Whitland Wales, who is supplying TMS coils for researchers to use.true2021Ultrafast photomodulation spectroscopy of photonic integrated circuitsA unique home-built instrument has been developed that allows ultrafast pump-probe spectroscopy of photonic integrated circuits. The instrument is referred to as the Ultrafast Photomodulation Spectroscopy or UPMS technique. In short, light is coupled into the photonic circuit using single-mode fibres and on-chip grating couplers. Typically this would be telecom light around 1550nm, but in principle any wavelength accessible by the laser system would be suitable with appropriate fibres. A second laser output is focused onto the circuit from the top using a scanning microscope objective. The setup allows mapping of the flow of light in the chip both in space, time, and frequency, thus producing hyperspectral datasets for full characterisation of the integrated optical components.Improvements to research infrastructureThe technique is still in the research phase and we are anticipating impact in coming years as we further developed the technique. It has been tested on a range of devices from the UK Cornerstone Silicon Photonics foundry. Also it has been developed for use in combination with laser annealing of Ge-implanted waveguides.true2015https://www.nature.com/articles/nphoton.2014.313Small moleculesIdentification of small molecules that purge the cells of mutant mitochondrial genome and restore mitochondrial function.Technology assay or reagentOne of the molecules tested in control animals has shown promising results in increasing mitochondrial functionality. Currently, we are conducting further testing in cells carrying various types of mutated mitochondrial DNA and in mouse models of disease to evaluate its efficacy in relevant contexts. Additionally, our team is in the process of preparing a clinical trial to assess the effects of this compound on a cohort of patients with mitochondrial DNA disorders. This represents an important step towards translating our preclinical findings into potential therapeutic interventions for human diseases. Finally, our team recently published a comprehensive review on this topic in a high-profile journal, consolidating the current understanding of mitochondrial DNA disorders and highlighting potential therapeutic strategies.true2021Nucleotide measurementAS group has developed a novel non-radioactive method for precise measurement of DNA synthesis building blocks (nucleotides) in both the cytosol and mitochondria. The scientists have validated its accuracy by comparing results with the established radioactive assay.Technology assay or reagentThis method has enabled the researchers to measure the levels of nucleotides in tissues of mice subjected to potential therapeutic interventions identified by AS group. The results are included in a manuscript submitted for publication to a high-profile journal. The new method is also used to assess the effect of the small molecules that select mtDNA on the nucleotide pool. The expectation is that the method efficiency and reliability will lead to its widespread adoption.false2023A New Device for In-Situ Measurement of Lubricant Viscosity using a Matching LayerA device that allows measurement of viscosity inside a vessel, tan, or pipeline with no direct contact between the sensor and sample. Based on the reflection of shear polarised ultrasound and the use of a matching layer to aid transmission.Improvements to research infrastructureIn progressfalseDevice for lubrication measurement in rolling bearingsUltrasonic measurement system for determining direct lubricant flow around a rolling bearing. Of particular use in wind turbines bearings. Evaluated in Barnesmore field wind turbine.Improvements to research infrastructurePhD student sponsored by Timken. Evaluated on data from Barnesmore field wind turbinetrue2020Method for measurement of oil and grease layers in rolling bearing inlet and outlet regionsUsing ultrasonic resonance to determine layer thickness around a bearing. This helps decide how well a bearing is lubricated as this residual film controls the oil film between rollers and raceways.Improvements to research infrastructurenone yet.false2020Device for load measurement in rolling bearingsUltrasonic measurement system for determining direct load application between rollers and raceways in rolling element bearings. Of particular use in wind turbines bearings. Evaluated in Barnesmore field wind turbine.Improvements to research infrastructureLicenced patent to Ricardo. Used to help design of main shaft bearing for Siemens Gamesa.true2014In-situ ultrasonic device for measuring battery state of charge and state of healthUltrasonic sensors bonded onto a lithium ion battery. Sound waves reflect back from the various interfaces sin a battery. This new research tool helps to determine what aspects fo the reflected signal give the most information about the battery electrode state. This is correlated to a batter charge during normal and abusive charge cycles.Improvements to research infrastructureNone yettrue2021The E3ME-FTT-GENIE simlation-based Integrated Assessment Model for informing climate policyThrough a collaboration between the UK's open University, Cambridge University, Cambridge Econometrics and Radboud University (NL), a new integrated assessment model (IAM) for informing climate policy-making was developed, coordinated in large parts by myself as a result of my EPSRC fellowship. This model is arguably the most advanced in its category, since no other model is fully based on simulation and fully calibrated by observational data, while it has the highest resolution of all IAMs used by the international community (IPCC). This model is made of the FTT family of technology evolution models, the E3ME macroeconoemtric model, and the GENIE or GENIE-PLASIM carbon cycle and climate models of intermediate complexity. This makes our IAM the only fully dynamical simulation of human-environmental processes.Model of mechanisms or symptoms - humanImpacts are to be seen in the coming years. For now, it resulted in being invited to several Horizon 2020 consortia bidding for funds, and being included in the international climate change modelling community. This is very new, and therefore public information on this model is still scarce, however we have a new article in press fully describing this model, to appear in the journal Energy Strategy Reviews, while three submissions are under review in Nature Climate Change resulting from this work.true2018Transgenic modelTransgenic model of two-fold elevated Phlda2Model of mechanisms or symptoms - mammalian in vivoIn addition to publications, meeting presentations and public/healthcare presentation, findings from this new model underpinned applications for a PhD studentship (internal funding) and two external grants (BBSRC J015156) and MRC grant (letter pending).false