Developing ways of implementing biocatalytic hydrogenation reactions in flow
Lead Research Organisation:
University of Oxford
Department Name: Interdisciplinary Bioscience DTP
Abstract
Widely applicable approaches to using NADH-dependent enzymes for highly selective redox steps in fine chemical synthesis are still lacking. This project exploits a novel H2-driven method for NAD(P)H recycling in which all enzymes are immobilised on carbon beads; this systems overcomes challenges with atom efficient cofactor recycling and simple enzyme handling, recovery and reuse. For these reasons, this system is also uniquely placed for translating biocatalysis into flow reactors, and thus establishes a new way of working that will be of great value in the industrial biotechnology sector.
BBSRC priorities areas and aims
This project sits firmly within the priority area of developing a "new strategic approach to industrial biotechnology" because it develops a methodology that will be broadly applicable to support uptake of biocatalysis for chemical synthesis. IBB, ENWW
BBSRC priorities areas and aims
This project sits firmly within the priority area of developing a "new strategic approach to industrial biotechnology" because it develops a methodology that will be broadly applicable to support uptake of biocatalysis for chemical synthesis. IBB, ENWW
Publications
Poznansky B
(2020)
Carbon as a Simple Support for Redox Biocatalysis in Continuous Flow.
in Organic process research & development
Poznansky B
(2021)
Boosting the Productivity of H2-Driven Biocatalysis in a Commercial Hydrogenation Flow Reactor Using H2 From Water Electrolysis
in Frontiers in Chemical Engineering
Studentship Projects
| Project Reference | Relationship | Related To | Start | End | Student Name |
|---|---|---|---|---|---|
| BB/M011224/1 | 01/10/2015 | 31/03/2024 | |||
| 1946990 | Studentship | BB/M011224/1 | 01/10/2017 | 30/09/2021 |
| Description | A continuous packed bed reactor for NADH-dependent biocatalysis using enzymes co-immobilized on a simple carbon support was optimized to 100% conversion in a residence time of 30 min. Conversion of pyruvate to lactate was achieved by co-immobilized lactate dehydrogenase and formate dehydrogenase, providing in situ cofactor recycling. Other metrics were also considered as optimization targets, such as low E factors between 2.5-11 and space-time yields of up to 22.9 g L-1 h-1. The long-term stability of the biocatalytic reactor was also demonstrated, with full conversion maintained over more than 30 h of continuous operation. |
| Exploitation Route | Spur on further research in the field. |
| Sectors | Environment,Manufacturing, including Industrial Biotechology,Pharmaceuticals and Medical Biotechnology |
| URL | https://pubs.acs.org/doi/10.1021/acs.oprd.9b00410 |
| Description | Dr Reddy's |
| Organisation | Dr. Reddy's Laboratories |
| Country | India |
| Sector | Private |
| PI Contribution | I have spent a month at Dr Reddy's as part of my DPhil Project. I will spend a further 2 months there, this year. |
| Collaborator Contribution | Dr Reddy's have provided lab space, equipment, and directional input on my project. They also provided some data for our joint publication. |
| Impact | 1 publication listed in the publication section |
| Start Year | 2017 |