The role of the endoplasmic reticulum in regulating T cell function
Lead Research Organisation:
UNIVERSITY OF CAMBRIDGE
Department Name: Medicine
Abstract
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Studentship Projects
| Project Reference | Relationship | Related To | Start | End | Student Name |
|---|---|---|---|---|---|
| BB/V509504/1 | 30/09/2020 | 29/09/2024 | |||
| 2439998 | Studentship | BB/V509504/1 | 30/09/2020 | 29/09/2024 | Katharina Patommel |
| Description | Youth Travel Fund |
| Amount | € 750 (EUR) |
| Funding ID | Y/21/001 |
| Organisation | Federation of European Biochemical Societies (FEBS) |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 04/2022 |
| End | 06/2022 |
| Description | Characterising the role of Aster proteins in shaping CD8+ T cell functionality using cholesterol transport inhibitors. |
| Organisation | Technical University of Denmark |
| Department | Department of Chemistry |
| Country | Denmark |
| Sector | Academic/University |
| PI Contribution | Using the supplied inhibitors to test how inhibition of different intracellular cholesterol transporters effects CD8+ T cell functionality (activation, cytokine production and proliferation). |
| Collaborator Contribution | Providing novel, unpublished inhibitors of intracellular cholesterol transport proteins. |
| Impact | Multidisciplinary: Dr. Laraia's lab is a Chemistry laboratory specialising in the development and characterisation of novel cholesterol transport inhibitors, whereas our lab specialises in immune-metabolism related research. |
| Start Year | 2023 |
| Description | Measuring the abundance of proteins involved in intracellular cholesterol transport and biosynthesis via protein mass spectrometry. |
| Organisation | University of Cambridge |
| Department | Department of Medicine |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Generation of cell pellets from Jurkat KO cell lines and primary T cells. Peptides used for assay development and as internal standards. |
| Collaborator Contribution | Assay development. |
| Impact | No outcomes yet - still in the development phase |
| Start Year | 2023 |
| Description | Structural and biochemical characterisation of Aster C PID variants |
| Organisation | Medical Research Council (MRC) |
| Department | MRC Laboratory of Molecular Biology (LMB) |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | I worked alongside a post-doc (Sisi Gao) in Prof. Modis' lab. We expressed and purified WT and mutant Aster C protein and subsequently characterised their respective stability as well as cholesterol binding/transfer capacities. |
| Collaborator Contribution | Molecular Dynamics modelling of WT and mutant human Aster C protein. Providing hands-on help (by Sisi Gao, a post-doc), guidance, lab space and reagents for the work described above. |
| Impact | Multi-Disciplinary: Structural Biology and Bioinformatics (Modis Lab) and Immuno-metabolism (Hess (our) lab). |
| Start Year | 2021 |
| Description | iCASE studentship (partnered with GSK) |
| Organisation | GlaxoSmithKline (GSK) |
| Country | Global |
| Sector | Private |
| PI Contribution | Hosting and supervising the PhD student (myself) who was selected to carry out the work for which this grant was awarded. |
| Collaborator Contribution | Contributing to the PhD student's stipend and towards some research costs/ travel for conferences. Supervision. Hosting the PhD student for 3 months (this has not happened yet). |
| Impact | Successful development of a CRISPR Cas9 KO approach for our genes of interest through advice from the industrial partner (Lee Booty, GSK). |
| Start Year | 2020 |