a state of the art facility for the study of protein trafficking in vivo
Lead Research Organisation:
University of Leeds
Department Name: Institute of Membrane & Systems Biology
Abstract
Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.
Technical Summary
Determining protein localisation and dynamics is important for answering many questions in biology. To understand how proteins function and are regulated in vivo, we need approaches by which we can determine where proteins go and when, as well as when and where two proteins interact. New and emerging technologies will go a long way towards helping us answer these questions. The first is the development of photo-activatable GFP (PA-GFP). By tagging proteins with PA-GFP, and then using photo-activation to observe a subset of fluorescently labelled molecules on a low fluorescent background, their fate can be accurately determined. The second is the recent developments in the GFP and RFP fluorophores that have improved behaviour in FRET, enabling us to use this approach to investigate protein-protein interactions in vivo. Furthermore, microscopy is now being developed as a tool for high throughput screening approaches, to investigate the effects of mutations, or for screening large numbers of small molecules for ones that have useful effects in cell biology. The major goal of this application is to upgrade our existing bio-imaging facility into a state-of-the-art facility that can exploit these new technologies, with the focus of studying protein trafficking in vivo.
Publications
Taneja T
(2009)
Sar1-GTPase-dependent ER exit of KATP channels revealed by a mutation causing congenital hyperinsulinism
in Human Molecular Genetics
Dalton J
(2006)
Intraepithelial ?d+ Lymphocytes Maintain the Integrity of Intestinal Epithelial Tight Junctions in Response to Infection
in Gastroenterology
Harrison SM
(2007)
Characterisation of cyclin D1 down-regulation in coronavirus infected cells.
in FEBS letters
Boyne JR
(2009)
Nucleolar disruption impairs Kaposi's sarcoma-associated herpesvirus ORF57-mediated nuclear export of intronless viral mRNAs.
in FEBS letters
Smith AJ
(2010)
Voltage-dependent charge movement associated with activation of the CLC-5 2Cl-/1H+ exchanger.
in FASEB journal : official publication of the Federation of American Societies for Experimental Biology
Ghosh SR
(2010)
Determination of the mobility of novel and established Caenorhabditis elegans sarcomeric proteins in vivo.
in European journal of cell biology
Hess K
(2012)
A novel mechanism for hypofibrinolysis in diabetes: the role of complement C3.
in Diabetologia
Griffiths RA
(2006)
Herpesvirus saimiri-based gene delivery vectors.
in Current gene therapy
Boyne JR
(2006)
gamma-2 Herpes virus post-transcriptional gene regulation.
in Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases
Li J
(2011)
Orai1 and CRAC Channel Dependence of VEGF-Activated Ca 2+ Entry and Endothelial Tube Formation
in Circulation Research
Naylor J
(2010)
Pregnenolone sulphate- and cholesterol-regulated TRPM3 channels coupled to vascular smooth muscle secretion and contraction.
in Circulation research
McHale R
(2010)
Prussian blue coordination polymer nanobox synthesis using miniemulsion periphery polymerization (MEPP)
in Chemical Communications
Dove BK
(2006)
Changes in nucleolar morphology and proteins during infection with the coronavirus infectious bronchitis virus.
in Cellular microbiology
Bahnasi YM
(2008)
Modulation of TRPC5 cation channels by halothane, chloroform and propofol.
in British journal of pharmacology
Henderson Z
(2010)
Co-localization of PRiMA with acetylcholinesterase in cholinergic neurons of rat brain: an immunocytochemical study.
in Brain research
Smith K
(2011)
Interactions between factor XIII and the aC region of fibrinogen
in Blood
Turrell SJ
(2012)
Cellular uptake of highly-functionalized ruthenium(II) tris-bipyridine protein-surface mimetics.
in Bioorganic & medicinal chemistry letters
Baker A
(2010)
Peroxisome biogenesis and positioning
in Biochemical Society Transactions
Hall K
(2010)
Unity and diversity in the human adenoviruses: exploiting alternative entry pathways for gene therapy
in Biochemical Journal
Xia R
(2008)
Inhibitory interaction between P2X4 and GABAC ?1 receptors
in Biochemical and Biophysical Research Communications
Smith AJ
(2006)
Increased ATP-sensitive K+ channel expression during acute glucose deprivation.
in Biochemical and biophysical research communications
Scott D
(2011)
Clot Architecture Is Altered in Abdominal Aortic Aneurysms and Correlates With Aneurysm Size
in Arteriosclerosis, Thrombosis, and Vascular Biology
| Description | The aim of this project was to improve our ability to use light microscopy to image cells, and within cells. The funding allowed us to buy additional equipment to upgrade our existing confocal microscopes, so that we could improve our imaging. These microscopes are used by over 20 different research groups within the Faculty of Biological Sciences at the University, and have supported a wide range of research, from imaging organelles and how they move in living plants, to imaging receptors in mammalian cells. |
| Exploitation Route | The research can be used by those interested in developing treatments for infections and disease (e.g. pharma companies, clinicians). Imaging is central to understanding the healthy human organism, plants and animals. Without knowledge of how things work, it is very difficult to understand what goes wrong in disease states. The new microscopes are essential for using imaging to understand cellular processes, and in detecting what goes wrong in diseases from virus infections, to inherited mutant |
| Sectors | Healthcare,Pharmaceuticals and Medical Biotechnology |
| URL | http://www.fbs.leeds.ac.uk/facilities/bioimaging/ |
| Description | This funding provided an upgrade to our bio-imaging facility which is used by over 40 different research groups across biological sciences and medicine. It has had impact in a broad range of healthcare and biological sciences. |
| First Year Of Impact | 2007 |
| Sector | Healthcare,Other |
| Impact Types | Economic |