Imaging of 3D Engineered Tissues

Lead Research Organisation: University of Sheffield
Department Name: Materials Science and Engineering

Abstract

Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.

Technical Summary

The current proposal outlines research themes on tissue engineering at Sheffield University where a laser scanning confocal microscope with META multichannel confocal detection is requested (with the potential for multiphoton upgrade in the future). The model is for a confocal system to be located in the new interdisciplinary Kroto Research Institute at Sheffield University. This facility will have a suite of GMP accredited clean rooms to enable tissue engineered construct formation under sterile conditions for clinical use. Multidisciplinary research laboratories located immediately next to the clean rooms will support core research and further development of reconstructed tissues. Location of an on-site confocal microscope in the research laboratories will therefore enable the in-depth study of cells in synthetic scaffolds across a number of research programmes. Confocal microscopy is a leading-edge technology and a powerful research tool, enabling the capture of unrivalled high resolution of cells within 3 dimensional constructs as well as in-depth penetration of specimens tagged with very specific fluorophores. This leading edge technology is now becoming a method of choice for the dynamic imaging of cells, tissues and biological systems not otherwise possible by other optical systems. Such a technology is essential for driving the research of our team, understanding a range of interconnected problems spanning the life sciences-engineering interface. Each member of our team has programmes that rely heavily on the need for high resolution microscopy which presently is only partially met by existing fluorescence systems. META confocal technology advances the quality of imaging and flexibility of analysis by such a high degree over standard fluorescence that it is now the new benchmark from visualising cells in vitro or in 3 dimension structures in vivo. It therefore has wide applicability in biology, chemistry and engineering and this is represented by our team of applicants. A central imaging facility will also be essential for maintaining research of an internationally competitive standard and will act as a potent catalyst for attracting high quality collaborations with investigators in the UK and abroad.

Publications

10 25 50

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Baggaley E (2014) Dinuclear ruthenium(II) complexes as two-photon, time-resolved emission microscopy probes for cellular DNA. in Angewandte Chemie (International ed. in English)

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Blackwood K (2008) Long term degradation study of electrospun scaffolds for soft tissue reconstruction in 8th World Biomaterials Congress 2008

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Botchway SW (2008) Time-resolved and two-photon emission imaging microscopy of live cells with inert platinum complexes. in Proceedings of the National Academy of Sciences of the United States of America

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Chunthapong J (2011) Development of a tissue-engineered skin model for detecting irritants in European Cells and Materials

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Chunthapong J (2008) A 3D Skin tissue-engineered model for inflammatory and toxicity testing in European Cells and Materials

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Hopper AP (2016) Photochemically modified diamond-like carbon surfaces for neural interfaces. in Materials science & engineering. C, Materials for biological applications

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Kaewkhaw R (2009) Adipose-derived stem cells (ASCs) for peripheral nerve repair in European Cells and Materials

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Kaewkhaw R (2011) Adipose-derived stem cells (ASCs) for peripheral nerve repair in European Cells and Materials

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Kaewkhaw R (2011) Adipose-derived stem cells for peripheral nerve repair in European Cells and Materials

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Morris HL (2010) Mechanisms of fluid-flow-induced matrix production in bone tissue engineering. in Proceedings of the Institution of Mechanical Engineers. Part H, Journal of engineering in medicine

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Murray-Dunning C (2009) The use of aligned polymer microfibres in peripheral nerve engineering in European Cells and Materials

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Murray-Dunning C (2008) Peripheral nerve engineering using aligned polymer microfibres in European Cells and Materials

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Murray-Dunning C (2008) Use of aligned polymer microfibres for peripheral nerve repair in 8th World Biomaterials Congress 2008

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Paviolo C (2013) Laser exposure of gold nanorods can increase neuronal cell outgrowth. in Biotechnology and bioengineering

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Sun T (2008) Development of a bioreactor for evaluating novel nerve conduits. in Biotechnology and bioengineering

 
Description The grant allowed the purchase of a versatile imaging system for conducting work on 3D in vitro cells/tissues. The major outcome has been the discovery of several methods for imaging a breadth of cells and scaffolds in 3D as a alternative to using animals.
Exploitation Route The grant has supported a tremendous breadth of outputs - not just those I am personally associated with (immediate outputs herein), but those of many other groups. These outputs have been documented on the webpage cited above and here - (https://www.sheffield.ac.uk/kroto/confocal).

These findings may be taken forward by devising new methods for further reducing animal research, new methods for SMEs to develop new implants, through to new bioreactors for such methods.
Sectors Healthcare

URL https://www.sheffield.ac.uk/kroto/confocal
 
Description This was an equipment grant for imaging 3D engineered tissues. It has led to a breadth of research represented in several outputs. Parallel findings have arisen including: 1. Design of commercial bioreactors in collaboration with SMEs 2. Design of methods for orthopaedic implants in collaboration with SMEs using 3D in vitro methods.
First Year Of Impact 2014
Sector Healthcare
Impact Types Economic