2-Photon Imaging: From Polymeric Materials to Engineered Tissues
Lead Research Organisation:
University of Sheffield
Department Name: Materials Science and Engineering
Abstract
Two-photon microscopy is a leading-edge imaging technology and a powerful research tool that combines long wavelength excitation and laser scanning microscopy. Of importance to our work it can enable capture of high resolution three dimensional images of living cells within 3D constructs as well as in-depth penetration of specimens tagged with very specific fluorophores. This technology is now becoming a method of choice for the dynamic imaging of biological and polymeric systems, not otherwise possible by other optical approaches and therefore will underpin a broad number of research programmes in biomaterials and tissue engineering.
Technical Summary
The current proposal is for the single purchase of a titanium-sapphire femtosecond pulsed NIR laser and a beam splitter to add to two existing confocal microscopes that are currently located in different departments. One system is inverted and equipped for fluorescence correlation spectroscopy (FCS) and the other is upright. Relocation of these will enable us to establish a single-site multiphoton / FCS imaging facility within the Kroto Research Institute at Sheffield University. This proposal outlines a number of highly developed and interconnected themes from 14 applicants that extend across the interface between the physical and life sciences, spanning polymeric biomaterials to tissue engineering, and justifies why a parallel two-photon confocal microscope facility with FCS is needed to develop our research programmes. This is most effectively answered by requesting money for a central multi-user facility that will have extensive multidisciplinary use and be based in a dedicated institute established for research across the disciplines.
Organisations
Publications
Zilic L
(2015)
An anatomical study of porcine peripheral nerve and its potential use in nerve tissue engineering.
in Journal of anatomy
Sun T
(2008)
Development of a bioreactor for evaluating novel nerve conduits.
in Biotechnology and bioengineering
Plenderleith RA
(2015)
Arginine-glycine-aspartic acid functional branched semi-interpenetrating hydrogels.
in Soft matter
Paviolo C
(2014)
Laser exposure of gold nanorods can induce intracellular calcium transients.
in Journal of biophotonics
Pateman CJ
(2015)
Nerve guides manufactured from photocurable polymers to aid peripheral nerve repair.
in Biomaterials
Murray-Dunning C
(2008)
Use of aligned polymer microfibres for peripheral nerve repair
in 8th World Biomaterials Congress 2008
Murray-Dunning C
(2008)
Peripheral nerve engineering using aligned polymer microfibres
in European Cells and Materials
Murray-Dunning C
(2009)
The use of aligned polymer microfibres in peripheral nerve engineering
in European Cells and Materials
Morris HL
(2010)
Mechanisms of fluid-flow-induced matrix production in bone tissue engineering.
in Proceedings of the Institution of Mechanical Engineers. Part H, Journal of engineering in medicine
Description | The grant allowed the purchase of a versatile imaging system for conducting work on 3D in vitro cells/tissues. The major outcome has been the discovery of several methods for imaging a breadth of cells and scaffolds in 3D as a alternative to using animals. |
Exploitation Route | The grant has supported a tremendous breadth of outputs - not just those I am personally associated with (immediate outputs herein), but those of many other groups. These outputs have been documented on the webpage cited above and here - (https://www.sheffield.ac.uk/kroto/confocal). These findings may be taken forward by devising new methods for further reducing animal research, new methods for SMEs to develop new implants, through to new bioreactors for such methods. |
Sectors | Healthcare |
URL | https://www.sheffield.ac.uk/kroto/confocal |
Description | This was an equipment grant for imaging 3D engineered tissues. It has led to a breadth of research represented in several outputs. Parallel findings have arisen including: 1. Design of commercial bioreactors in collaboration with SMEs 2. Design of methods for orthopaedic implants in collaboration with SMEs using 3D in vitro methods. |
First Year Of Impact | 2010 |
Sector | Healthcare |
Impact Types | Economic |