Bluetongue virus transmission between ruminant host and Culicoides vectors: the importance of insect saliva proteins

Lead Research Organisation: The Pirbright Institute
Department Name: UNLISTED

Abstract

Bluetongue virus (BTV) is an arthropod-borne virus (an Arbovirus) transmitted between its ruminant hosts by blood feeding 'biting' midges (Culicoides spp.). The virus can infect most ruminants but causes haemorrhagic fever and severe clinical disease, in sheep (especially European breeds), some species of deer and to a lesser extent in cattle. Until a decade ago bluetongue was a disease of warm climates, areas containing populations of transmission-competent insect vector species. However, since 1998 BTV has moved progressively further north into Europe, a process related to warming of the local climate and an expanding distribution of certain midge vector populations. In 2006, a bluetongue outbreak occurred much further north than ever before, affecting Belgium, the Netherlands, Germany, Luxembourg, and North East France. The virus causes serve clinical disease in sheep and even cattle and approximately 50% of sheep and 10% of cattle developing clinical signs die. This outbreak confirms that some northern European midge species (which are the same as in the UK) can transmit BTV, and demonstrates that the whole of Europe, including the UK, is now under threat of further outbreaks. However, not all species of midge can transmit BTV efficiently, suggesting that transmission of the virus and the distribution of the disease is influenced by the insect vectors and the strain of virus involved. Blood feeding insects deposit saliva into the skin of the host. This causes inflammation and hypersensitivity in the skin, which results in the familiar red spots and itching, or even allergic vesicles, after midge or mosquito bites. BTV is transmitted in the insect's saliva and must survive the inflammation response in order to infect the ruminant host. However, the saliva also contains proteases (to help prevent clotting) which can modify the virus, even increasing its infectivity for the insect vector. It is often assumed that Arboviruses are injected into the blood of the host by feeding insects. However, midges use their mouthparts to cut and damage skin tissues, so they can feed on a mixed pool of blood, tissue and lymphatic fluid. BTV is not injected into blood vessels but left in the saliva deposited into the skin. The virus can replicate in certain skin cells and additionally in cells of the hosts inflammatory response that are attracted to the biting site. Therefore BTV may be present at higher levels in the skin than in the bloodstream, which may be important for uptake by feeding midges. So it appears that the virus has evolved to make use of the saliva proteins and the host's response, to increase the efficiency of transmission, and its chances of survival. We have recently developed methods to isolate midge saliva proteins in large quantities, to investigate their role in BTV transmission between vector insects and ruminant hosts. Initial studies indicate that different midge species have different saliva proteins, which may help explain differences in their vector 'competence'. There are unique facilities at Pirbright to work on midges, sheep and the bluetongue virus itself, that will allow us to explore their relationships and influence on transmission mechanisms. The project will compare saliva from different European, African and American midges, to determine the role of saliva proteins in BTV infection in mammalian and insect tissue culture cells, or in the sheep. The project will elucidate the importance of locally generated virus in the skin for BTV transmission and will also determine if the virus can be transmitted mechanically between infected and non-infected sheep (by other insects or by needle), or directly from one midge to another while they feed on the same sheep. The results of this research will improve our understanding of BTV transmission in Europe, helping to define the risk of bluetongue transmission and to implement appropriate control measures.

Technical Summary

Bluetongue virus (BTV) can infect most ruminants but causes severe disease, in sheep, some species of deer and to a lesser extent in cattle. The virus is maintained in the field by transmission cycles between ruminant hosts and insect vectors - blood feeding midges (Culicoides spp.). Adults of most Culicoides have the potential to become infected with BTV. However their ability to transmit virus varies dramatically between species and only a small proportion of insects become infected (even from a known vector species). Any factors that can enhance infection of the insect, would also increase BTV transmission efficiency, and may influence vector status. In contrast, BTV transmission from vector to host is very efficient, requiring only a single insect bite to cause a severe clinical infection, while a much larger amount of virus is normally used to cause similar effects by needle inoculation. Culicoides saliva proteins cause a severe inflammatory response, and profound changes in the sheep skin, including formation of vesicles, synthesis (or release) of inflammatory modulators and massive recruitment of leukocytes. These changes may play an important part in the BTV infection mechanism in the sheep. Indeed cellular components of the ovine immune system can themselves become infected by BTV and may be involved in initial stages of infection and dissemination. Novel methods will be used to collect saliva proteins of Culicoides vector (and non-vector) species. Major protein components will be identified by AA sequencing. The effect of these proteins (particularly proteases) on virus structure and infectivity for different cells will be determined. The effect of saliva proteins and the inflammatory response, on virus titre and persistence in the sheep skin will be explored. The possibility of mechanical BTV transmission between host animals (by other insects, or by needle), or non-systemic transmission between co-feeding vector insects, will also be examined.

Publications

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Description Culicoides biting midge saliva can directly cleave the outer coat protein VP2 of bluetongue virus (BTV) and thereby directly modify the virus particle. This treatment increases the infectivity of BTV particles 10 fold for insect-vector cells, enhancing transmission to vector insects. Furthermore the saliva of some midge species is more efficient than others, suggesting that the constitution of Culicoides saliva proteins might be involved in vector competence.

Collaborative work during this grant showed that skin dendritic cells are involved in the initial dissemination of BTV in the host.

Transmission studies demonstrated that if needles are re-used for subcutaneous inoculations, Bluetongue virus (BTV) can be mechanically transmitted in sheep and cattle, although these less invasive techniques are normally considered low risk.

Mechanical transmission of BTV did not occur after allowing non-vector biting insects (stable fly Stomoxis calcitrans) to partially blood-feed on an infected sheep, followed by immediately completing the blood-feeding on naïve recipient sheep.

BTV can be orally transmitted to ruminants with infection efficiency depending on the cell culture system used to generate the virus. BTV generated in mammalian cell culture was much more efficient in infecting cattle and sheep via the oral route compared to the same virus strain propagated in insect cell cultures. However, the virus generated in insect cell cultures is highly infectious and virulent when inoculated subcutaneously into susceptible ruminates, suggesting that BTVs might differ in their ability to infect specific cell targets, depending on the cell culture system used to generate the virus.
Exploitation Route The research carried out under this funding has led to new research areas at The Pirbright Institute further investigating the influence of arthropod saliva on arbovirus transmission, infectivity and virulence. The specific effect of Culicoides saliva proteins on bluetongue virus infectivity and transmission has generated considerable interest and other international research teams have carried out in vivo infection studies of ruminants in the context of insect vector transmission. Additionally the methodology of Culiocides saliva protein collection has also been utilised by other international research teams and further proteins in C. sonorensis saliva have been identified.
Sectors Agriculture, Food and Drink

 
Description The work and results of this study have direct implications for the methods of vaccine delivery to reduce the spread of bluetongue virus, as a result of vaccination campaigns. These results have been communicated to DEFRA.
First Year Of Impact 2012
Sector Agriculture, Food and Drink