High-field NMR Spectroscopy for Biomolecular Research
Lead Research Organisation:
University of Bristol
Department Name: Chemistry
Abstract
NMR spectroscopy is the most information-rich analytical technique available to molecular research, but requires a high level of instrumentation to maximise the benefits of such analyses. In particular, its' application to materials of biological interest requires high quality instrumentation, with powerful superconducting magnets for signal resolution (500MHz or greater) and maximum sensitivity for proton nuclei. The Bristol NMR Facility currently supports a substantial number of researchers in the Biomolecular field and current NMR hardware is no longer sufficient to fulfil the demands of the cutting edge research projects conducted - which covers areas such as biosynthesis, protein structure and dynamics, potential disease treatments (Alzheimers and Cystic Fybrosis), bionanotechnology and membrane protein-ligand interactions. To this end, a modern 500MHz automated NMR spectrometer is required enabling these research fields to take advantage of the latest hardware and experimental developments required to achieve their aims.
Technical Summary
Biomolecular research at Bristol has a high demand for high resolution, high-sensitivity 1H-based NMR spectroscopy which cannot be fulfilled with current hardware. Over 60 biomolecular researchers require routine access to >400MHz spectroscopy in a range of projects spanning biosynthesis, protein structure and dynamics, bionanotechnology, membrane protein-ligand interactions, pharmaceutical development and ion transport in cell membranes. A 1H-sensitive, triple resonance 500MHz automated spectrometer will allow these research workers routine access to modern experimental methods (HSQC, field-gradient NOE, etc) at sufficiently high resolution for their needs. The rapid access to molecular structure elucidation, dynamics measurements and binding studies will allow more efficient and effective research programmes to be progressed and new projects to be developed at Bristol.
Publications
Ndukwe I
(2015)
Pure-shift IMPRESS EXSIDE - Easy measurement of 1 H- 13 C scalar coupling constants with increased sensitivity and resolution
in RSC Advances
Bame J
(2018)
Improved NOE fitting for flexible molecules based on molecular mechanics data - a case study with S -adenosylmethionine
in Physical Chemistry Chemical Physics
Amer MM
(2018)
Substituted Dihydroisoquinolinones by Iodide-Promoted Cyclocarbonylation of Aromatic a-Amino Acids.
in Organic letters
Ackrill TD
(2015)
Synthesis of Diarylheptanoid Scaffolds Inspired by Calyxins I and J.
in Organic letters
Ganesh V
(2018)
Chiral Aniline Synthesis via Stereospecific C(sp3)-C(sp2) Coupling of Boronic Esters with Aryl Hydrazines.
in Organic letters
Mooibroek TJ
(2016)
Synthesis and evaluation of a desymmetrised synthetic lectin: an approach to carbohydrate receptors with improved versatility.
in Organic & biomolecular chemistry
Niwetmarin W
(2018)
(-)-Cytisine: Access to a stereochemically defined and functionally flexible piperidine scaffold.
in Organic & biomolecular chemistry
Iqbal Z
(2018)
Investigations into the biosynthesis of the antifungal strobilurins.
in Organic & biomolecular chemistry
Costil R
(2018)
a-Methyl phenylglycines by asymmetric a-arylation of alanine and their effect on the conformational preference of helical Aib foldamers.
in Organic & biomolecular chemistry
Nofiani R
(2018)
Strobilurin biosynthesis in Basidiomycete fungi
in Nature Communications
Fawcett A
(2019)
Carbopalladation of C-C s-bonds enabled by strained boronate complexes.
in Nature chemistry
Burns M
(2014)
Assembly-line synthesis of organic molecules with tailored shapes.
in Nature
Leonard DJ
(2018)
Asymmetric a-arylation of amino acids.
in Nature
Harper MJ
(2018)
Oxidative Addition, Transmetalation, and Reductive Elimination at a 2,2'-Bipyridyl-Ligated Gold Center.
in Journal of the American Chemical Society
Wu J
(2018)
Photoinduced Deaminative Borylation of Alkylamines.
in Journal of the American Chemical Society
Wang GW
(2018)
Modular Access to Azepines by Directed Carbonylative C-C Bond Activation of Aminocyclopropanes.
in Journal of the American Chemical Society
Farndon JJ
(2018)
Stereospecific Alkene Aziridination Using a Bifunctional Amino-Reagent: An Aza-Prilezhaev Reaction.
in Journal of the American Chemical Society
Mykura RC
(2018)
Investigation of the Deprotonative Generation and Borylation of Diamine-Ligated a-Lithiated Carbamates and Benzoates by in Situ IR spectroscopy.
in Journal of the American Chemical Society
Fawcett A
(2019)
Strain-Release-Driven Homologation of Boronic Esters: Application to the Modular Synthesis of Azetidines.
in Journal of the American Chemical Society
Grélaud S
(2018)
Branch-Selective and Enantioselective Iridium-Catalyzed Alkene Hydroarylation via Anilide-Directed C-H Oxidative Addition.
in Journal of the American Chemical Society
Singleton WG
(2017)
Convection enhanced delivery of panobinostat (LBH589)-loaded pluronic nano-micelles prolongs survival in the F98 rat glioma model.
in International journal of nanomedicine
Dalling AG
(2018)
Synthesis of Nitrogen Heterocycles via Directed Carbonylative C-C Bond Activation of Cyclopropanes.
in Chimia
Ndukwe IE
(2016)
EXtended ACquisition Time (EXACT) NMR-A Case for 'Burst' Non-Uniform Sampling.
in Chemphyschem : a European journal of chemical physics and physical chemistry
Tassoti S
(2018)
High Resolution for Chemical Shifts and Scalar Coupling Constants: The 2D Real-Time J-Upscaled PSYCHE-DIAG
in ChemPhysChem
Adam C
(2018)
The Role of Terminal Functionality in the Membrane and Antibacterial Activity of Peptaibol-Mimetic Aib Foldamers
in Chemistry - A European Journal