High-field NMR Spectroscopy for Biomolecular Research
Lead Research Organisation:
University of Bristol
Department Name: Chemistry
Abstract
NMR spectroscopy is the most information-rich analytical technique available to molecular research, but requires a high level of instrumentation to maximise the benefits of such analyses. In particular, its' application to materials of biological interest requires high quality instrumentation, with powerful superconducting magnets for signal resolution (500MHz or greater) and maximum sensitivity for proton nuclei. The Bristol NMR Facility currently supports a substantial number of researchers in the Biomolecular field and current NMR hardware is no longer sufficient to fulfil the demands of the cutting edge research projects conducted - which covers areas such as biosynthesis, protein structure and dynamics, potential disease treatments (Alzheimers and Cystic Fybrosis), bionanotechnology and membrane protein-ligand interactions. To this end, a modern 500MHz automated NMR spectrometer is required enabling these research fields to take advantage of the latest hardware and experimental developments required to achieve their aims.
Technical Summary
Biomolecular research at Bristol has a high demand for high resolution, high-sensitivity 1H-based NMR spectroscopy which cannot be fulfilled with current hardware. Over 60 biomolecular researchers require routine access to >400MHz spectroscopy in a range of projects spanning biosynthesis, protein structure and dynamics, bionanotechnology, membrane protein-ligand interactions, pharmaceutical development and ion transport in cell membranes. A 1H-sensitive, triple resonance 500MHz automated spectrometer will allow these research workers routine access to modern experimental methods (HSQC, field-gradient NOE, etc) at sufficiently high resolution for their needs. The rapid access to molecular structure elucidation, dynamics measurements and binding studies will allow more efficient and effective research programmes to be progressed and new projects to be developed at Bristol.
Publications
Niwetmarin W
(2018)
(-)-Cytisine: Access to a stereochemically defined and functionally flexible piperidine scaffold.
in Organic & biomolecular chemistry
Costil R
(2018)
a-Methyl phenylglycines by asymmetric a-arylation of alanine and their effect on the conformational preference of helical Aib foldamers.
in Organic & biomolecular chemistry
Jones CR
(2011)
Accuracy in determining interproton distances using Nuclear Overhauser Effect data from a flexible molecule.
in Beilstein journal of organic chemistry
Dias CM
(2018)
Anthracene Bisureas as Powerful and Accessible Anion Carriers.
in Chemistry (Weinheim an der Bergstrasse, Germany)
Burns M
(2014)
Assembly-line synthesis of organic molecules with tailored shapes.
in Nature
Leonard DJ
(2018)
Asymmetric a-arylation of amino acids.
in Nature
Lister FGA
(2018)
Bis-pyrene probes of foldamer conformation in solution and in phospholipid bilayers.
in Chemical science
Grélaud S
(2018)
Branch-Selective and Enantioselective Iridium-Catalyzed Alkene Hydroarylation via Anilide-Directed C-H Oxidative Addition.
in Journal of the American Chemical Society
Dalling AG
(2019)
Carbonylative C-C Bond Activation of Electron-Poor Cyclopropanes: Rhodium-Catalyzed (3+1+2) Cycloadditions of Cyclopropylamides.
in Angewandte Chemie (International ed. in English)
Fawcett A
(2019)
Carbopalladation of C-C s-bonds enabled by strained boronate complexes.
in Nature chemistry
Wu J
(2019)
Catalyst-Free Deaminative Functionalizations of Primary Amines by Photoinduced Single-Electron Transfer
in Angewandte Chemie International Edition
Szwalbe A
(2019)
Characterisation of the biosynthetic pathway to agnestins A and B reveals the reductive route to chrysophanol in fungi
in Chemical Science
Ganesh V
(2018)
Chiral Aniline Synthesis via Stereospecific C(sp3)-C(sp2) Coupling of Boronic Esters with Aryl Hydrazines.
in Organic letters
Singleton WG
(2017)
Convection enhanced delivery of panobinostat (LBH589)-loaded pluronic nano-micelles prolongs survival in the F98 rat glioma model.
in International journal of nanomedicine
Armstrong RJ
(2018)
Enantiodivergent Synthesis of Allenes by Point-to-Axial Chirality Transfer.
in Angewandte Chemie (International ed. in English)
Ríos P
(2017)
Enantioselective carbohydrate recognition by synthetic lectins in water.
in Chemical science
Mas-Roselló J
(2018)
Enantioselectively functionalised phenytoin derivatives by auxiliary-directed N to C aryl migration in lithiated a-amino nitriles.
in Chemical communications (Cambridge, England)
Rubial B
(2018)
Enantiospecific Synthesis of ortho -Substituted 1,1-Diarylalkanes by a 1,2-Metalate Rearrangement/ anti -S N 2' Elimination/Rearomatizing Allylic Suzuki-Miyaura Reaction Sequence
in Angewandte Chemie International Edition
Silvi M
(2018)
Enantiospecific Three-Component Alkylation of Furan and Indole
in Chemistry - A European Journal
Ndukwe IE
(2016)
EXtended ACquisition Time (EXACT) NMR-A Case for 'Burst' Non-Uniform Sampling.
in Chemphyschem : a European journal of chemical physics and physical chemistry
Butts CP
(2011)
High precision NOEs as a probe for low level conformers--a second conformation of strychnine.
in Chemical communications (Cambridge, England)
Tassoti S
(2018)
High Resolution for Chemical Shifts and Scalar Coupling Constants: The 2D Real-Time J-Upscaled PSYCHE-DIAG
in ChemPhysChem
Bame J
(2018)
Improved NOE fitting for flexible molecules based on molecular mechanics data - a case study with S -adenosylmethionine
in Physical Chemistry Chemical Physics
Mykura RC
(2018)
Investigation of the Deprotonative Generation and Borylation of Diamine-Ligated a-Lithiated Carbamates and Benzoates by in Situ IR spectroscopy.
in Journal of the American Chemical Society
Iqbal Z
(2018)
Investigations into the biosynthesis of the antifungal strobilurins.
in Organic & biomolecular chemistry