High-field NMR Spectroscopy for Biomolecular Research

Lead Research Organisation: University of Bristol
Department Name: Chemistry

Abstract

NMR spectroscopy is the most information-rich analytical technique available to molecular research, but requires a high level of instrumentation to maximise the benefits of such analyses. In particular, its' application to materials of biological interest requires high quality instrumentation, with powerful superconducting magnets for signal resolution (500MHz or greater) and maximum sensitivity for proton nuclei. The Bristol NMR Facility currently supports a substantial number of researchers in the Biomolecular field and current NMR hardware is no longer sufficient to fulfil the demands of the cutting edge research projects conducted - which covers areas such as biosynthesis, protein structure and dynamics, potential disease treatments (Alzheimers and Cystic Fybrosis), bionanotechnology and membrane protein-ligand interactions. To this end, a modern 500MHz automated NMR spectrometer is required enabling these research fields to take advantage of the latest hardware and experimental developments required to achieve their aims.

Technical Summary

Biomolecular research at Bristol has a high demand for high resolution, high-sensitivity 1H-based NMR spectroscopy which cannot be fulfilled with current hardware. Over 60 biomolecular researchers require routine access to >400MHz spectroscopy in a range of projects spanning biosynthesis, protein structure and dynamics, bionanotechnology, membrane protein-ligand interactions, pharmaceutical development and ion transport in cell membranes. A 1H-sensitive, triple resonance 500MHz automated spectrometer will allow these research workers routine access to modern experimental methods (HSQC, field-gradient NOE, etc) at sufficiently high resolution for their needs. The rapid access to molecular structure elucidation, dynamics measurements and binding studies will allow more efficient and effective research programmes to be progressed and new projects to be developed at Bristol.

Publications

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Iqbal Z (2018) Investigations into the biosynthesis of the antifungal strobilurins. in Organic & biomolecular chemistry

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Nofiani R (2018) Strobilurin biosynthesis in Basidiomycete fungi in Nature Communications

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Leonard DJ (2018) Asymmetric a-arylation of amino acids. in Nature

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Wu J (2018) Photoinduced Deaminative Borylation of Alkylamines. in Journal of the American Chemical Society

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Ndukwe IE (2016) EXtended ACquisition Time (EXACT) NMR-A Case for 'Burst' Non-Uniform Sampling. in Chemphyschem : a European journal of chemical physics and physical chemistry

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Silvi M (2018) Enantiospecific Three-Component Alkylation of Furan and Indole in Chemistry - A European Journal

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Dias CM (2018) Anthracene Bisureas as Powerful and Accessible Anion Carriers. in Chemistry (Weinheim an der Bergstrasse, Germany)

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Stewart P (2018) Maltodextrin recognition by a macrocyclic synthetic lectin. in Chemical communications (Cambridge, England)

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Butts CP (2011) High precision NOEs as a probe for low level conformers--a second conformation of strychnine. in Chemical communications (Cambridge, England)

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Cooper P (2018) Iridium-Catalyzed a-Selective Arylation of Styrenes by Dual C-H Functionalization in Angewandte Chemie International Edition

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Abrams R (2018) Transition Metal Free Cycloamination of Prenyl Carbamates and Ureas Promoted by Aryldiazonium Salts. in Angewandte Chemie (International ed. in English)

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Carter TS (2016) Platform Synthetic Lectins for Divalent Carbohydrate Recognition in Water. in Angewandte Chemie (International ed. in English)

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Armstrong RJ (2018) Enantiodivergent Synthesis of Allenes by Point-to-Axial Chirality Transfer. in Angewandte Chemie (International ed. in English)