High-field NMR Spectroscopy for Biomolecular Research
Lead Research Organisation:
University of Bristol
Department Name: Chemistry
Abstract
NMR spectroscopy is the most information-rich analytical technique available to molecular research, but requires a high level of instrumentation to maximise the benefits of such analyses. In particular, its' application to materials of biological interest requires high quality instrumentation, with powerful superconducting magnets for signal resolution (500MHz or greater) and maximum sensitivity for proton nuclei. The Bristol NMR Facility currently supports a substantial number of researchers in the Biomolecular field and current NMR hardware is no longer sufficient to fulfil the demands of the cutting edge research projects conducted - which covers areas such as biosynthesis, protein structure and dynamics, potential disease treatments (Alzheimers and Cystic Fybrosis), bionanotechnology and membrane protein-ligand interactions. To this end, a modern 500MHz automated NMR spectrometer is required enabling these research fields to take advantage of the latest hardware and experimental developments required to achieve their aims.
Technical Summary
Biomolecular research at Bristol has a high demand for high resolution, high-sensitivity 1H-based NMR spectroscopy which cannot be fulfilled with current hardware. Over 60 biomolecular researchers require routine access to >400MHz spectroscopy in a range of projects spanning biosynthesis, protein structure and dynamics, bionanotechnology, membrane protein-ligand interactions, pharmaceutical development and ion transport in cell membranes. A 1H-sensitive, triple resonance 500MHz automated spectrometer will allow these research workers routine access to modern experimental methods (HSQC, field-gradient NOE, etc) at sufficiently high resolution for their needs. The rapid access to molecular structure elucidation, dynamics measurements and binding studies will allow more efficient and effective research programmes to be progressed and new projects to be developed at Bristol.
Publications
Rubial B
(2018)
Enantiospecific Synthesis of ortho -Substituted 1,1-Diarylalkanes by a 1,2-Metalate Rearrangement/ anti -S N 2' Elimination/Rearomatizing Allylic Suzuki-Miyaura Reaction Sequence
in Angewandte Chemie International Edition
Bame J
(2018)
Improved NOE fitting for flexible molecules based on molecular mechanics data - a case study with S -adenosylmethionine
in Physical Chemistry Chemical Physics
Mykura RC
(2018)
Investigation of the Deprotonative Generation and Borylation of Diamine-Ligated a-Lithiated Carbamates and Benzoates by in Situ IR spectroscopy.
in Journal of the American Chemical Society
Dalling AG
(2018)
Synthesis of Nitrogen Heterocycles via Directed Carbonylative C-C Bond Activation of Cyclopropanes.
in Chimia
Ganesh V
(2018)
Chiral Aniline Synthesis via Stereospecific C(sp3)-C(sp2) Coupling of Boronic Esters with Aryl Hydrazines.
in Organic letters
Tassoti S
(2018)
High Resolution for Chemical Shifts and Scalar Coupling Constants: The 2D Real-Time J-Upscaled PSYCHE-DIAG
in ChemPhysChem
Leonard DJ
(2018)
Asymmetric a-arylation of amino acids.
in Nature
Stewart P
(2018)
Maltodextrin recognition by a macrocyclic synthetic lectin.
in Chemical communications (Cambridge, England)
Iqbal Z
(2018)
Investigations into the biosynthesis of the antifungal strobilurins.
in Organic & biomolecular chemistry
Pelšs A
(2018)
Reoptimization of the Organocatalyzed Double Aldol Domino Process to a Key Enal Intermediate and Its Application to the Total Synthesis of ?12 -Prostaglandin J3.
in Chemistry (Weinheim an der Bergstrasse, Germany)