A pipeline for rapid cloning of stem rust resistance genes effective against Ug99 from wild diploid wheat relatives

Lead Research Organisation: University of East Anglia
Department Name: Sainsbury Laboratory

Abstract

Wheat is arguably the most important cereal crop in the world, with more than 600 million tons produced annually, supplying greater than nineteen percent of human dietary calories. The limited genetic diversity of wheat however renders it vulnerable to new diseases. The wheat stem rust fungus, known as the 'polio of agriculture', has caused repeated widespread crop failures throughout recorded history in North America, Europe, Asia and Australia. In the 1950s Norman Borlaug, father of the Green Revolution, successfully bred for resistance to the disease. This resistance held until the end of the '90's, when a new, super-virulent race of wheat stem rust called Ug99 emerged in Africa. Ug99 is capable of causing disease on greater than ninety percent of the world's wheat varieties. First detected in Uganda, it has spread at an alarming rate through sub-Saharan Africa and across the Arabian Peninsula, appearing in Iran in 2008. Because wind-borne rust spores can travel long distances, it is only a matter of time before this scourge reaches Pakistan and India, the source of nineteen percent of the world's wheat and home to 1 billion people. Changing climate will expose Europe to enhanced risk of the disease. New sources of stem rust resistance are urgently needed. Although traditional breeding can introduce new genes into wheat from other related species, this process is laborious, time-consuming, and difficult to control, often causing the simultaneous introduction of deleterious characteristics along with the desired trait ('linkage drag'). Moreover, when new resistance genes are deployed one-at-a-time, the pathogen typically overcomes the resistance gene within one or two growing seasons, rendering it useless. This problem can be alleviated by introducing more than one resistance gene at a time, but that turns out to be impractical if not impossible by conventional breeding methods. The work proposed here aims to identify new resistance genes from a wild relative of wheat, Aegilops sharonensis. This grass, native to the Levant, is closely related to one of wheat's progenitors, and contains a rich trove of new, unexploited resistance genes. Our long-term strategy is to isolate, by molecular cloning, as many new resistance genes as possible from this species, and introduce them in combinations using GM methods. Molecular cloning makes it possible, indeed straightforward, to put several new genes together in the same location in the genome, allowing breeders to work with them as a 'single' gene. This holds tremendous advantages for disease resistance breeding, and is a clear case where GM technology is not only vastly superior to conventional breeding, but indeed required for sustainable food security. Our proposal has the specific goals of i) cloning the first of these genes, based on preliminary genetic mapping information already in hand, and ii) developing a novel method for quickly identifying the position in the genome of any gene of interest. This novel method will use a combination of classical genetics and 'next-generation' ultra-high-throughput sequencing technology, which now makes experiments that would have been prohibitively expensive only a few years ago both feasible and affordable. The platform we are developing will have as a primary output new lines of wheat that harbour three or more new stem rust resistance genes at a single genetic locus. These lines will be made available to public breeding programs that develop new breeding material for developing countries in harm's way from stem rust. Aegilops also harbors genetic diversity for other useful traits, including water and nitrogen use efficiency. Thus, the genomic data and methodology we will develop in this project will benefit wheat improvement generally.

Technical Summary

This project aims to identify and isolate genes responsible for resistance to the wheat stem rust fungus Puccinia graminis f. sp. tritici, Pgt from the wild wheat relative Aegilops sharonensis. Pgt has become a paramount problem in agriculture because new races of the pathogen (Ug99 and related strains) have appeared that can overcome nearly all genetic resistances currently deployed in wheat. Ae. sharonensis contains several novel resistance (R) genes that function against Ug99. Starting with already-available mapping populations segregating for resistance genes, we propose to genetically map and clone these genes. We will use established map-based cloning methods that take advantage of the synteny among monocot genomes, which we have validated for Ae. sharonensis, barley, and Brachypodium. We also propose to develop a novel method for rapidly fine-mapping R genes based on using oligonucleotide capture and next-generation sequencing. This strategy focuses on the class of nucleotide binding leucine-rich repeat-containing (NB-LRR) genes, which comprise roughly 90% of the known R genes. Specifically, we propose to: i) obtain deep genome sequence coverage of Ae. sharonensis using next-generation (Illumina) sequencing ii) identify and assemble candidate NB-LRR gene sequences from these data iii) design an oligonucleotide capture array that selects for NB-LRR genes iv) create bulked genomic DNA preparations from homozygous resistant and homozygous susceptible individuals derived from a mapping population v) carry out target enrichment using the NB-LRR capture array on the DNA from the bulks vi) perform next-generation sequencing of NB-LRR-enriched DNA from the bulks and identify single nucleotide polymorphisms that co-segregate with the genes of interest vii) test candidate genes identified by stable transformation of wheat

Planned Impact

A durable solution to wheat stem rust is a paramount problem in global food security. Because the wheat stem rust pathogen is evolving rapidly and has shown the ability to overcome each new resistance gene that is deployed in agriculture, developing a capability to isolate large numbers of useful genes and deploy them in combinations is critical to successfully overcome this disease. The proposed research aims to do exactly that: develop new methods for rapidly accessing novel resistance genes that can be deployed in wheat breeding programs. Direct beneficiaries of this work include public wheat germplasm improvement programs (such as those at CIMMYT), the country-level breeding efforts that they support in the developing world (including Kenya, Ethiopia, Pakistan, India, and China), growers of wheat, and consumers of wheat in those countries. In the absence of a durable solution to wheat stem rust, wheat agriculture, particularly in Africa and South Asia, will live under the constant threat of epidemics and resulting crop failure caused by this disease. The genetic materials developed in this project, transgenic wheat lines containing multiple resistance genes effective against stem rust, will be available in three to five years. In addition, although stem rust has not been important in recent years in UK or European wheat production, climate change brings a new threat from this disease, which requires relatively warm conditions early in the growing season to have full impact. Although in high-value agriculture the pathogen can be controlled with chemical fungicide application, genetic resistance is vastly preferable from the standpoints of cost and environmental sustainability. Thus, UK and European wheat breeding companies, wheat growers, and consumers are potential direct beneficiaries of this work as well. The timescale for these benefits to be realized is uncertain, because we do not yet know the trajectory of stem rust's progression in Europe and the UK. Aegilops sharonensis, our choice as source for these novel resistance genes, is of interest to the global community of wheat researchers because its genome is closely related to the B genome of hexaploid bread wheat. The progenitors of the other components of the wheat genome are already being sequenced by BGI (China), so our effort in this project to generate a draft sequence of Ae. sharonensis will ideally complement these other resources. The combined tools of deep sequence and vastly improved genetic map of this organism will enable any interested researcher to more easily identify genes associated with useful agronomic traits in Ae sharonenesis. In addition to resistance genes for other diseases, these will include genes for tolerance to abiotic stresses such as heat and drought, and increased efficiency of nutrient utilization. These resources will be available to the research community as soon as we develop them, largely in the first year of the project, so their impact will be felt immediately. The research staff working on this project will develop additional technical expertise in a diverse range of skills, including molecular genetics, bioinformatics, and plant biotechnology, specifically in wheat, an exceedingly important crop in UK agriculture. They will also develop an increased understanding of the application of how basic science discoveries, such as the cloning of new genes envisioned in this project, get translated into practical outcomes for agriculture by working with our downstream deployment partners. This expertise can be applied in future career positions in academia, research institutes, and in companies, both small agricultural biotech firms and global multi-national agriculture companies. These benefits will be realized immediately upon the next career progression steps taken by the individuals involved in the project.

Publications

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Wulff BB (2011) Improving immunity in crops: new tactics in an old game. in Current opinion in plant biology

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Marcussen T (2014) Ancient hybridizations among the ancestral genomes of bread wheat. in Science (New York, N.Y.)

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International Wheat Genome Sequencing Consortium (IWGSC) (2014) A chromosome-based draft sequence of the hexaploid bread wheat (Triticum aestivum) genome. in Science (New York, N.Y.)

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Steuernagel B (2015) NLR-parser: rapid annotation of plant NLR complements. in Bioinformatics (Oxford, England)

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Kawashima CG (2016) A pigeonpea gene confers resistance to Asian soybean rust in soybean. in Nature biotechnology

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Yu G (2017) Discovery and characterization of two new stem rust resistance genes in Aegilops sharonensis. in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik

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Hickey LT (2019) Breeding crops to feed 10 billion. in Nature biotechnology

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Hao Y (2020) Harnessing Wheat Fhb1 for Fusarium Resistance. in Trends in plant science

 
Title 2nd Prize in Engaging Images Art Competition, The Sainsbury Centre for Visual Arts 
Description Josh Waites' artwork based on the MARPLE wheat rust diagnostic tool from the @Saunders_Lab came second in the Engaging Images Art Competition. Due to MARPLE's use in Ethiopia, he painted it in a traditional orthodox Ethiopian art style. It was displayed at The Sainsbury Centre for Visual Arts during the month of December 2019. 
Type Of Art Artwork 
Year Produced 2019 
Impact Not aware of any. 
URL https://twitter.com/JoshOWaites/status/1203311578571059200
 
Title Blog on "Wild wheat, plant genomics, and food security" 
Description I wrote a blog for the Global Engage conference "Plant Genomics and Gene Editing Congress: Europe". 
Type Of Art Creative Writing 
Year Produced 2020 
Impact The blog was well received and widely tweeted. 
URL http://www.global-engage.com/agricultural-biotechnology/wild-wheat-plant-genomics-and-food-security/
 
Title Wrestling genes out of the giant barley and wheat genomes 
Description Blog for general science audience on how to rapidly clone genes from wheat or barley 
Type Of Art Creative Writing 
Year Produced 2016 
Impact Postive feedback from friends and colleagues. 
URL http://blogs.biomedcentral.com/on-biology/2016/10/24/wrestling-genes-out-of-the-giant-barley-and-whe...
 
Description Maximizing the genetic potential of bread wheat requires a deep understanding of the structure and function of its genome, including the evolutionary relationship between the wheat homoeologous genomes and their progenitors and relatives.

Towards this goal we have made two key deliverables as part of this Research Grant:

(1) We developed a simple and generic three-step process that allows cloning (identifying) a gene from the hexaploid wheat genome (containing 126,000 genes) associated with a defined disease resistance trait. We used our novel method to clone an important gene conferring resistance to worldwide isolates of the causal agent of wheat stem rust (Puccinia graminis f.sp. tritici). This technology puts our longterm research programme on a more straightforward path to success as it will allow cloning multiple disease resistance genes and combining them in a transgene cassette - a strategy that should provide more durable genetic disease resistance (and less reliance on chemical disease control). We have drafted a manuscript on this work which was recently accepted for publication in Nature Biotechnology.

(2) We sequenced the genome of a wild grass related to wheat. This helped depict the evolutionary relationship between the three sub genomes of hexaploid bread wheat and its progenitor species. These findings were reported recently in two Science papers (July 2014).

Note added: 8 March 2019: We have since cloned approximately nine resistance genes from wheat using MutRenSeq. For example, we recently published on the cloining of the first NLR stripe rust resistance genes to be cloned from wheat, namely Yr5, Yr7 and YrSp (Marchal et al., Nature Plants, 2018). We have also helped many colleagues to clone their favourite gene, and others in the community have used our method to clone genes in wheat, e.g. Pm21 (Xing et al., Molecular Plant 2018). We also improved our method to make it unbiased by uncoupling the mutational genomics from RenSeq and coupling it to chromosome flow sorting and sequencing. The resulting application, MutChromSeq, allowed us to clone Pm2 from wheat and Eceriferum-q from barley (Sanchez-Martin et al., 2016, Genome Biology) and Sr43 from wheat (Yu et al., unpublished). One limitation of mutational genomics, however, is the requirement for generating and screening large numbers of mutant progeny. This is impractical for most wild, undomesticated relatives of our crops, due to long generation times and a poor agronomy which prevents mechanised seed harvest and processing. We therefore coupled resistance gene enrichment sequencing (RenSeq) to association genetics on a genetically diverse panel of wild wheats to take advantage of the naturally occuring genetic structure (historical recombinations and mutations) to rapidly clone a suite of resistance genes (Arora et al., 2019, Nature Biotechnology). We therefore now have a suite of complementary methods for rapidly cloning resistance genes from complex genomes.
Exploitation Route Our gene cloning technique can be used to clone defined disease resistance genes from complex uncharacterised crop genomes, not just wheat, in a timely fashion and independently of marker discovery and recombination. This will allow facile and economical cloning multiple resistance genes towards a given disease and stacking (pyramiding) them for more durable resistance.
Sectors Agriculture, Food and Drink,Environment

 
Description Our key finding, the novel wheat gene cloning technology, has now been accepted for publication in Nature Biotechnelogy. We have filed for IP protection on the genes we have cloned using this approach. We recently published a manuscript in Theoretical and Applied Genetics stemming from this work. Note added 08 March 2019 and updated March 2020: Two of the stem rust resistance genes we cloned through this project, namely Sr22 and Sr45, have been incorporated into a multi-stem rust resistance gene stack (containing 5 Sr genes) and transformed into wheat by our collaborator Mick Ayliffe, CSIRO, Australia. The GM lines were tested during the 2017/2018 and 2018/2019 growing seasons in the field in Minnesota by our colleague Brian Steffenson and proved to be completely immune to the local races of stem rust. Our project coordinator, The 2Blades Foundation, are exploring the possibility of testing the stacks in the field in Kenya. Moreover, in collaboration with Mick Ayliffe, 2Blades are making additional stacks incorporating other Sr genes which we have since cloned, which we predict will provide more durable resistance. I am hopeful that this work, with time, could help pave the way for GM resistance gene pyramids in wheat and other crops.
First Year Of Impact 2015
Sector Agriculture, Food and Drink,Education
Impact Types Societal

 
Description Science Media Centre's briefing notes on GM plants
Geographic Reach National 
Policy Influence Type Implementation circular/rapid advice/letter to e.g. Ministry of Health
URL http://www.sciencemediacentre.org/wp-content/uploads/2012/09/SMC-Briefing-Notes-GM-Crops.pdf
 
Description A multi-R gene stack for durable resistance to wheat stem rust (with 2Blades Foundation)
Amount £939,147 (GBP)
Organisation Two Blades Foundation 
Sector Charity/Non Profit
Country United States
Start 07/2014 
End 06/2017
 
Description BBSRC "Impact Acceleration Account"
Amount £13,078 (GBP)
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 09/2017 
End 02/2018
 
Description BBSRC - ISCF Agri-food Technology Catalyst
Amount £29,943 (GBP)
Funding ID BB/SCA/JIC/17 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 11/2017 
End 02/2018
 
Description BBSRC iCASE
Amount £113,000 (GBP)
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 10/2018 
End 09/2022
 
Description Cloning and characterization of Sr43 and stem rust resistance genes SrA, SrB, SrC and SrD from Aegilops sharonensis
Amount £100,165 (GBP)
Organisation Two Blades Foundation 
Sector Charity/Non Profit
Country United States
Start 07/2019 
End 10/2020
 
Description Doctoral Trainingship Programme
Amount £97,000 (GBP)
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 10/2017 
End 09/2021
 
Description Global Challenges Research Fund
Amount £316,622 (GBP)
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 10/2017 
End 07/2018
 
Description Marie Sklodowska Curie Action International Training Network
Amount € 238,507 (EUR)
Organisation Marie Sklodowska-Curie Actions 
Department Initial Training Networks (ITN)
Sector Charity/Non Profit
Country Global
Start 01/2016 
End 12/2019
 
Description Newton Institutional Links Grant
Amount £299,973 (GBP)
Organisation Newton Fund 
Sector Public
Country United Kingdom
Start 04/2018 
End 03/2020
 
Description OWWC gene cloning
Amount £20,000 (GBP)
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 10/2019 
End 03/2020
 
Description Safeguarding our daily bread from wheat rust diseases
Amount £2,930 (GBP)
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 06/2019 
End 08/2019
 
Description Universiti Putra Malaysia International PhD Scholarship
Amount £116,308 (GBP)
Organisation Putra Malaysia University 
Sector Academic/University
Country Malaysia
Start 10/2013 
End 09/2017
 
Title Aegilops sharonensis structured populations 
Description We have assembled a large collection of wild Aegilops sharonensis (Sharon goatgrass) samples, a wild weedy relative of wheat from the Levant. Our collection includes a cores diversity set (~133 lines) from across the geographical range, ~100 crosses and F2 populations, and two F6 recombinant inbred line populations (with ~100 individuals per population). This unique collection could facilitate the identification and cloning of genes that could be used improve the genetic basis of wheat. 
Type Of Material Biological samples 
Provided To Others? No  
Impact Too early to tell. 
 
Title EMS + RenSeq cloning of disease resistance genes 
Description There are 126,000 protein coding genes in the wheat genome. Probably ~1000 of these are homologues of resistance genes belonging to the nucleotide binding site leucine-rich repeat (NB-LRR) family. Attributing a certain disease resistance characteristic to one of these is a huge challenge. We have developed a generic method that allows cloning a defined NB-LRR resistance gene based on complexity reduction of a NextGen sequencing library and applying this to a genetically structured population. The technology, albeit developed for wheat, should be applicable to other complex crop genomes. 
Type Of Material Technology assay or reagent 
Provided To Others? No  
Impact We have not yet published our method so it is too early to tell. 
 
Description Anticipatory Breeding and Characterization of Stem Rust Mutants with Altered Virulence Phenotypes 
Organisation Two Blades Foundation
Country United States 
Sector Charity/Non Profit 
PI Contribution Pipeline for sequencing wheat stem rust mutants.
Collaborator Contribution Generation of EMS-derived wheat stem rust mutants for identification of Avr-effectors.
Impact Putative wheat stem rust (Puccinia graminis f. sp. tritici) virulent mutants have been generated for one defined Sr gene. These five mutants will be bulked up for retesting and sequencing in 2014.
Start Year 2013
 
Title METHODS FOR IDENTIFYING PLANT PATHOGEN RESISTANCE GENES 
Description Methods are provided for identifying a plant disease resistance (R) gene for a plant disease of interest. The methods involve using bait sequences to select a subgroup of nucleic acids from a group of nucleic acids that are derived from a mutagenized plant that is susceptible to the plant disease of interest but that was produced by mutagenizing a plant that is resistant to the disease of interest. The bait sequences are designed to hybridize to one or more genes from at least one plant R gene family. The methods further involve sequencing the subgroup of nucleic acids to obtain a collection of nucleic acid sequences, comparing such nucleic acid sequences with corresponding sequences of one or more genes that are derived from a resistant plant, and identifying at least one nucleic acid sequence derived from the mutagenized plant that is not identical in sequence to a corresponding sequence from the resistant plant. Further provided are related methods for identifying a gene associated with a phenotypic change for a trait of interest in plants and other organisms. 
IP Reference US20150240233 
Protection Patent application published
Year Protection Granted 2015
Licensed No
Impact The cloning of two disease resistance genes from wheat effective against wheat stem rust. The participation in many collaborative projects to clone wheat disease resistance genes against wheat stem rust, stripe rust, leaf rust, powdery mildew, and other diseases.
 
Title Wheat stem rust resistance genes and methods of use 
Description The patent describes the cloning and characterisation of two stem rust resistance genes in wheat. 
IP Reference WO2017024053 
Protection Patent application published
Year Protection Granted 2015
Licensed No
Impact The genes have been added to multi-R gene stacks and transformed into wheat and barley. These will be field trialled in Minnesota, USA in the 2018 growing season.
 
Title AgRenSeq - GitHub 
Description AgRenSeq is a pipeline to identify candidate resistance (R) genes in plants directly from a diversity panel. The diversity panel needs to be sequenced (R gene enrichment sequencing - RenSeq) and phenotyped. Phenotype scores need to be converted to AgRenSeq scores that assign positive values to resistance and negative values to suscetibility. An intermediate phenotype should have an AgRenSeq score close to zero. For RenSeq you will need a bait library that targets R genes in your plant species. A bait library for Aegilops tauschii can be found here. We reccomend Arbor biosciences for synthesis of baits. They also offer the enrichment service. 
Type Of Technology Software 
Year Produced 2018 
Impact Requests for collaboration from academia and industry. 
 
Title MutRenSeq Mutant Hunter 
Description The MutRenSeq Mutant Hunter is a pipeline to combine resistance gene enrichment sequencing (RenSeq) with EMS mutant screens to identify NLR-type resistance genes. 
Type Of Technology Software 
Year Produced 2015 
Open Source License? Yes  
Impact This pipeline has resulted in collaborations (some on responsive mode grants), research agreements, and further funding (e.g. from the 2Blades Foundation, USA). 
URL https://github.com/steuernb/MutantHunter/
 
Description 5th Plant Genomics Workshop, Amsterdam 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Presented my work on rapid gene cloning and speed breeding at the 5th Plant Genomics Workshop, Amsterdam. This lead to questions and discussion with other scientists and representatives from industry, and I was invited back to present again in 2018, as well as invited to the advisory board.
Year(s) Of Engagement Activity 2017
 
Description Advance in battle against wheat pest - BBC News? (June 2013) 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Media (as a channel to the public)
Results and Impact I was interviewed by a journalist from the BBC and this resulted in a citation in an online article on the BBC website.

Not known.
Year(s) Of Engagement Activity 2013
URL http://www.bbc.co.uk/news/science-environment-23084782
 
Description Annual Science Meeting 2018, JIC and TSL 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact I was called upon to give an update of my work at the JIC and TSL Annual Science Meeting 2018.
Year(s) Of Engagement Activity 2018
 
Description Annual Science Meeting, John Innes Centre and TSL, Norwich, UK 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact I gave a presentation at the JIC/TSL annual science meeting, which sparked lots of questions and discussion.

This presentation resulted in interest from a representative of a seed breeding company based in the UK.
Year(s) Of Engagement Activity 2012
 
Description BMGF Grand Challenges Meeting, Berlin, Germany 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I was invited to the BMGF Grand Challenges Meeting, in Berlin, Germany. This has since led to further interactions with the BMGF and DFID funded DGGW project, and a request for a workshop at the next DGGW meeting which will be held in the John Innes Centre in 2020.
Year(s) Of Engagement Activity 2019
 
Description CerealPath PhD Course on Scientific Soft Skills 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact I co-organised and taught a course on scientific soft skills for a group of PhD students from the EU funded CerealPath programme.
Year(s) Of Engagement Activity 2016
URL https://cerealpath.eu/
 
Description Cranfield University 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Postgraduate students
Results and Impact I was called upon to give a lecture to MSc students on the Cranfield University bioinformatics course. I was asked to come back again in May for a two day visit to teach scientific soft skills for early career scientists.
Year(s) Of Engagement Activity 2019
 
Description DivSeek Workshop 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact I was called upon at short notice to give a presentation at the DivSeek workshop on how to exploit germplasm collections for rapid trait identificaiton and deployment.
Year(s) Of Engagement Activity 2019
 
Description GM Wheat "a life saver" - The Sunday Times 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact I was interviewed by a reporter from the Sunday Times about my work on wheat stem rust and the use of GM technologies in crops. The article appeared a few days later.

This article stimulated a lot of feedback from colleagues, friends and family.
Year(s) Of Engagement Activity 2011
URL http://www.thesundaytimes.co.uk/sto/news/uk_news/Science/article810143.ece
 
Description Group member Postdoc Kumar Gaurav invited to present Keynote Lecture at EuroBiotech, Kraków, Poland, 2019 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Keynote Lecture, EuroBiotech, Kraków, Poland, 2019
Year(s) Of Engagement Activity 2020
URL https://www.eurobiotech.krakow.pl/gb/program-x/program.html
 
Description How to the feed the 7 Billion - The Sunday Times 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Media (as a channel to the public)
Results and Impact I was interviewed by a reporter representing the Sunday times.

An article appeared in the Sunday Times. I got lots of feedback from colleagues, friends and family.
Year(s) Of Engagement Activity 2011
URL http://www.thesundaytimes.co.uk/sto/newsreview/features/article809155.ece
 
Description Interview by Jerome Starkey, The Times 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Media (as a channel to the public)
Results and Impact Following our press release on the appearance of stem rust in the UK for the first time in 50 years, I was interviewed by The Times correspondent Jereme Starkey. This resulted in a short article in The Times.
Year(s) Of Engagement Activity 2018
URL https://www.thetimes.co.uk/article/plague-that-scared-the-romans-is-back-g6nzm5d0k
 
Description Interview by MBC TV 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Media (as a channel to the public)
Results and Impact I was interviewed by a reporter from MBC TV, a Middle East news channel, about my work on wheat stem rust and cloning genes against Ug99.

Not known.
Year(s) Of Engagement Activity 2012
URL http://www.mbc.net/ar/programs/mbc-news/videos/video-render.play-e2dce9d4-c2bf-4aea-b09b-d2f615c517a...
 
Description Interview with Newsweek on genetic diversity 
Form Of Engagement Activity A magazine, newsletter or online publication
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Media (as a channel to the public)
Results and Impact I was interviewed by Newsweek on the importance of genetic diversity in crops.
Year(s) Of Engagement Activity 2018
URL http://www.newsweek.com/feed-world-scientists-have-plan-save-chickpeas-destruction-806925
 
Description Interviews with press and media on speed breeding 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact JIC External Relations department released a press release on our work on 'speed breeding'. This sparked considerable attention from the press and media. I expect I gave about 20 interviews in the ensuing days, featured on BBC Look East, the local press, two national newspapers (The Times and The Guardian), Newsweek, as well as countless other media outlets across the world, including in Japanese, Chinese and Arabic.
Year(s) Of Engagement Activity 2018
 
Description Invitation to present work at Wiersum Plantbreeding, The Netherlands 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact I was invited to visit Wiersum Plantbreeding in the Netherlands. We discussed potential future collaborative work around the concept of a resistance gene atlas for wheat.
Year(s) Of Engagement Activity 2019
 
Description Invited presentaiton (plenary session) at the Molecular Plant Microbe Interactions congress in Glasgow 2019 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I was invited to give a presentation of my work at the 2019 MPMI meeting in Glasgow. Based on this, I have since received two additional invitations to give seminars in Oxford University and Edinburgh University.
Year(s) Of Engagement Activity 2019
URL https://www.ismpmi.org/Congress/Pages/default.aspx
 
Description Invited presentaiton at G20 Wheat Meeting in Frankfurt 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I was invited to give a presentation at the G20 wheat meeting in Frankfurt. The presentation sparked questions and led to requests for futher information and collaboration.
Year(s) Of Engagement Activity 2016
URL https://www.bmel.de/SharedDocs/TermineVeranstaltungen/EN/16-12-08-InternationalWheatCongress.html
 
Description Invited presentation at 7th Central European Congress of Life Sciences Eurobiotech, Krakow, Poland 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact My postdoc Kumar Gaurav was invited to give a Keynote Lecture at the the 7th Central European Congress of Life Sciences Eurobiotech, Krakow, Poland.
Year(s) Of Engagement Activity 2019
URL https://www.eurobiotech.krakow.pl/gb/
 
Description Invited presentation at AgriTech East, NIAB, Cambridge, UK 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Industry/Business
Results and Impact AgriTech East dissemination activity to local farmers, breeders and industry stake holders on rust diseases of wheat.
Year(s) Of Engagement Activity 2016
URL https://www.cambridgenetwork.co.uk/events/pollinator-rusts-and-other-cereal-killers/
 
Description Invited presentation at BASF, Belgium 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact I was invited to BASF in Ghent, Belgium to present my work on rapid gene discovery and cloning in crops. We later discussed potential collaborations. This has resulted in further requests for information and discussions on collaboration.
Year(s) Of Engagement Activity 2020
 
Description Invited presentation at BeCA-ILRI (Nairobi, Kenya) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact This presentation sparked questions and discussions.

Expansion of my scientific network and opportunities for future collaborations.
Year(s) Of Engagement Activity 2014
 
Description Invited presentation at Beijing Genomics Institute, Shenzhen, China 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact ~20 scientist from the Beijing Genomics Institute (BGI) attended my scientific presentation and this sparked further interest and discussion.

Periodic request for further information.
Year(s) Of Engagement Activity 2012
 
Description Invited presentation at Elsoms Ltd, Spalding 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Industry/Business
Results and Impact I was invited to give a presentation to the vegatble and herb breeding company Elsoms Ltd in Spalding, Lincolnshire. The presentation lead to request for further information and
Year(s) Of Engagement Activity 2016
 
Description Invited presentation at Institute of Botany, Chinese Academy of Sciences, Beijing, China 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact I gave a presentation to ~20 members of the Genetics Department at the Beijing Genomics Institute, which sparked questions and further debate.

Strengthening of network with Chinese scientists.
Year(s) Of Engagement Activity 2012
 
Description Invited presentation at Institute of Experimental Botany, Czech Republic 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I was invited to the Institute of Experimental Botany in the Czech Republic to give a presentation about my work on rapid gene discovery and cloning in Triticeae.
Year(s) Of Engagement Activity 2018
 
Description Invited presentation at KAUST, Thuwal, Saudi Arabia 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact I visited KAUST to present my work. This has resulted in offers of further collaboration.
Year(s) Of Engagement Activity 2020
 
Description Invited presentation at Kew 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact I was invited to give a short seminar on how to exploit genetic variation in crop wild relatives at a joint workshop organised between the John Innes Centre and Kew.
Year(s) Of Engagement Activity 2018
 
Description Invited presentation at Key's Symposium, Keygene, Netherlands 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact I was invited to give a presentation on my work at Keygene, The Netherlands. This resulted in requests for further information and interactions.
Year(s) Of Engagement Activity 2019
 
Description Invited presentation at Max Planck Institute for Plant Breeding Research, Cologne, Germany 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact I gave a presentation to ~25 members of the MPIZ, which sparked questions and discussion.

This resulted in heightened interest in my work.
Year(s) Of Engagement Activity 2013
 
Description Invited presentation at National Institute of Botany 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact I gave a presentation on next generation disease resistance breeding in wheat.
Year(s) Of Engagement Activity 2015
 
Description Invited presentation at Plant Sciences Department, University of Oxford 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Postgraduate students
Results and Impact I was invited to present my work at the Plant Sciences Department at the University of Oxford.
Year(s) Of Engagement Activity 2019
 
Description Invited presentation at Pwani Univeristy, Kenya 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact As part AfriPlantSci, a two-week course on plant sciences for African scientists, I was invited to give a presentation at Pwani University, Kenya describing my research on rapid gene cloning and speed breeding in cereals.
Year(s) Of Engagement Activity 2019
URL https://acaciaafrica.org/afriplantsci/
 
Description Invited presentation at Technical University of Munich 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I was invited to give a presentation at the TUM on my speed breeding and rapid gene cloning work in cereals.
Year(s) Of Engagement Activity 2019
URL https://portal.mytum.de/termine/Event.2019-03-20.1464449129/event_view?
 
Description Invited presentation at The Sainsbury Laboratory 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact I was invited to present my work in The Sainsbury Laboratory spring science meeting.
Year(s) Of Engagement Activity 2019
 
Description Invited presentation at The Sainsbury Laboratory, Norwich, UK 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Was invited to give a presentation of my work on wheat rusts at the annual Sainsbury Lab retreat.
Year(s) Of Engagement Activity 2016
 
Description Invited presentation at University College Dublin 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact I gave a presentaiton to undergraduates and research scientists on the use of mutational genomics to isolate disease resistance genes.
Year(s) Of Engagement Activity 2016
 
Description Invited presentation at University of Munich, Faculty of Biology, Germany 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact Approximately 30 scientists attended my presentation which sparked further discussion and thinking.

Heightened interest in wheat stem rust and the use of GM crops.
Year(s) Of Engagement Activity 2012
 
Description Invited presentation at University of Nottingham 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Postgraduate students
Results and Impact I was invited to give a presentation on my work at the University of Nottingham to share my latest results on rapid resistance gene discovery, cloning and deployment. This resulted in long discussions.
Year(s) Of Engagement Activity 2019
 
Description Invited presentation at the 8th Plant Genomics & Gene Editing Congress, Rotterdam, The Netherlands 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I was invited to present my work on rapid gene discovery and cloning, and generation advancement at the 8th Plant Genomics & Gene Editing Congress, Rotterdam, The Netherlands.
Year(s) Of Engagement Activity 2020
URL http://www.global-engage.com/event/plant-genomics/
 
Description Invited presentation at the Australian National University, Canberra, Australia 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Professional Practitioners
Results and Impact I gave a presentation on cloning and stacking stem rust resistance genes in wheat.
Year(s) Of Engagement Activity 2015
 
Description Invited presentation at the Bill and Melinda Gates Foundation Grand Challenges Meeting in London 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I was asked to give a presentation on molecular breeding for durable disease resistance, including my work on speed breeding and speed cloning. The presentation sparked considerable interest and I was asked to chair two round table discussions on the topic where I answered many questions and was requested additional information.
Year(s) Of Engagement Activity 2016
URL http://grandchallenges.org/videos
 
Description Invited presentation at the Institute of Cereal Crop Research, Tel Aviv, Israel 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact Gave a presentation on the use of genetic diversity from wild grasses to improve wheat disease resistance
Year(s) Of Engagement Activity 2016
 
Description Invited presentation at the Ludwig Maximilian University of Munich 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact I was invited to give a presentation at the LMU about my work on rapid gene isolation and speed breeding in cereals.
Year(s) Of Engagement Activity 2019
 
Description Invited presentation at the Molecular Plant Microbes Interaction Meeting in Portland, USA 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I was invited to give a presentation on my work on speed breeding and wheat rusts at the biennial congress on Molecular Plant-Microbe Interactions.
Year(s) Of Engagement Activity 2016
URL http://www.ismpmi.org/Congress/2016/MeetingSchedule/Pages/default.aspx
 
Description Invited presentation at the University of Copenhagen 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact I gave a presentation on the cloning of stem rust resistance genes from wheat and wild relatives and their potential use in transgenic wheat.
Year(s) Of Engagement Activity 2015
 
Description Invited presentation at the University of Nairobi, Nairobi, Kenya 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact I gave a presentation to ~10 members of the Faculty of Agriculture, University of Nairobi, which sparked questions and discussion afterwards.

This resulted in requests for further information and involvement.
Year(s) Of Engagement Activity 2014
 
Description Invited presentation at the Wheat Durable Resistance Meeting, Minneapolis, USA 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I was invited to give a presentation at the Wheat Durable Resistance Meeting in Minneapolis, USA. The presentation lead to debate and request for additional information and collaboration.
Year(s) Of Engagement Activity 2016
URL https://www.ag.ndsu.edu/wheatresistance
 
Description Invited presentation at the Wheat Initiative Expert Working Group, Minneapolis, USA 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I was invited to join the wheat Expert Working Group on Control of Wheat Pathogens and Pests and deliver a presentation on available genomics and germplasm resources and what the community needs in this respect. The presentation lead to debate and requests for germplasm sharing with other labs.
Year(s) Of Engagement Activity 2016
URL http://www.wheatinitiative.org/activities/expert-working-groups/control-wheat-pathogens-and-pests
 
Description Invited presentation at the wheat breeding company KWS 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact I visited the wheat breeding company KWS at their Cambridge site and gave a presentation on how to use mutational genomics approaches to identify markers that can be used in breeding. The presentation sparked interest and generated significant debate.
Year(s) Of Engagement Activity 2015
 
Description Invited presentation by lab member PhD student Sreya Ghosh at Plant and Animal Genome Conference, 2020. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact My PhD student Sreya Ghosh was invited to present at PAG, 2020 in San Diego. This resulted in request for additional information and has led to the drafting of a patent application with the 2Blades Foundation.
Year(s) Of Engagement Activity 2020
 
Description Keynote Speaker, Advances in Integrated Pest Management, Association of Applied Biologists, Harper Adams University, UK 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact I was invited to be a Keynote Speaker, at the meeting entitled Advances in Integrated Pest Management, Association of Applied Biologists, Harper Adams University, UK. This led to futher request to be involved in a responsive mode GCRF collaboration, which regretfully was not funded.
Year(s) Of Engagement Activity 2017
 
Description Keynote Speaker, German Plant Breeding Society, Wernigerode, Germany 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I was the Keynote Speaker, German Plant Breeding Society, Wernigerode, Germany. This resulted in additional interest from a range of colleagues in academia and industry, including policy makers.
Year(s) Of Engagement Activity 2018
 
Description PhD students in India, Ethiopia and Kenya to fight wheat stripe rust - PhysOrg (January 2014) 
Form Of Engagement Activity A magazine, newsletter or online publication
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Media (as a channel to the public)
Results and Impact The TSL/JIC Communications team made a press release and this resulted in several online publications.

Not known.
Year(s) Of Engagement Activity 2014
URL http://phys.org/wire-news/151316776/phd-students-in-india-ethiopia-and-kenya-to-fight-wheat-stripe-r...
 
Description Plant Genomics and Gene Editing Congress, Rotterdam, The Netherlands 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact I was invited to present at a Congress in Rotterdam.
Year(s) Of Engagement Activity 2018
 
Description Plant scientists to explore genetics to halt spread of crop disease - Agro News 
Form Of Engagement Activity A magazine, newsletter or online publication
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Media (as a channel to the public)
Results and Impact The JIC/TSL Communications team released a press release which resulted in several online publications by organisations interested in GM crops.

Not known
Year(s) Of Engagement Activity 2011
URL http://news.agropages.com/News/NewsDetail---5960.htm
 
Description Plenary Speaker, 4th International Conference on Plant Genetics, Genomics, Bioinformatics and Biotechnology, Almaty, Kazakhstan 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I was invited to present my work on speed cloning and speed breeding as a Plenary Speaker at the 4th International Conference on Plant Genetics, Genomics, Bioinformatics and Biotechnology, Almaty, Kazakhstan. This led to stimulating discussions and contributed to requests for further involvment.
Year(s) Of Engagement Activity 2017
 
Description Postdoc member Kumar Gaurav invited to present at Monogram Workshop, 2019 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Invited presentation at the Monogram Workshop, 2019.
Year(s) Of Engagement Activity 2020
 
Description Presentation at 4th International Conference "Plant Biotic Stresses & Resistance Mechanisms", Vienna, Austria 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I presented my work at the 4th International Conference "Plant Biotic Stresses & Resistance Mechanisms", Vienna, Austria.
Year(s) Of Engagement Activity 2020
URL http://viscea.org/plant-biotic-stresses-resistance-mechanisms-iv-february-19-20-2020/
 
Description Presentation at Institute of Genetics and Developmental Biology, Beijing, China 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I was invited to visit the Institute of Genetics and Developmental Biology, Beijing, China and present my work on speed cloning and speed breeding. This has led to follow up requests for further visits, collaboration, and applicaiton for joint funding opportunities.
Year(s) Of Engagement Activity 2017
 
Description Presentation at International Wheat Conference, Saskatoon, Canada 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact MINING A SEQUENCE-CONFIGURED WILD WHEAT DIVERSITY PANEL FOR GENES REGULATING ENVIRONMENTAL RESPONSE USING ASSOCIATION GENETICS
Year(s) Of Engagement Activity 2019
 
Description Presentation at John Innes Centre Wheat Breeders Day 2019 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact We presented a talk on "Mining wild wheat diversity for disease resistance". This sparked questions and discussions. I have been asked to visit BASF in Gent, Belgium to discuss potential collaboration.
Year(s) Of Engagement Activity 2019
 
Description Presentation at Nanjing Agricultural University, China 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I was invited to Nanjing Agricultural University, China where I gave a presentation on my work on speed cloning and speed breeding. I was invited to participate in further collaborations based on this visit.
Year(s) Of Engagement Activity 2017
 
Description Presentation at Science Innovation Showcase, JIC, UK 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact I was invited to present at the Science Innovation Showcase, JIC, UK. This has led to requests for further information and ongoing disussions with inustrial partners for collaboration / contract research.
Year(s) Of Engagement Activity 2018
 
Description Presentation at Tel Aviv University, Israel 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I was invited to present my work on speed breeding and speed cloning at Tel Aviv University, Israel. This led to further discussions and resulted in an active collaboration and exchange of germplasm.
Year(s) Of Engagement Activity 2017
 
Description Presentation to African journalists, JIC, April 2014 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Media (as a channel to the public)
Results and Impact I gave a presentation on wheat stem rust and potential GM solutions to ~30 journalists from East and West Africa. This sparked questions and discussion afterwards.

This resulted in various online articles in African forums.
Year(s) Of Engagement Activity 2014
 
Description Presentation to Angolan Ministry of Agriculture 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Policymakers/politicians
Results and Impact I gave a presentation on wheat stem rust and potential GM solutions to representatives of the Angolan Ministry of Agriculture, including Antonio David Dias de Silva, General Director, Institute of Industrial Development and Engracia Manuel, Chief of Head Office, Ministry of Agriculture. This sparked further questions and discussion.


Not known.
Year(s) Of Engagement Activity 2014
 
Description Presentation to Appolinaire Dijkeng and Jacob Mignouna, BecA 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I gave a presentation to Appolinaire Dijkeng and Jacob Mignouna from the BecA-ILRI Hub in Nairobi, Kenya. This was part of ongoing discussions and collaborative work between JIC and BecA-ILRI.
Year(s) Of Engagement Activity 2017
 
Description Presentation to DFID, May 2012 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Policymakers/politicians
Results and Impact I gave a presentation GM crop technology/strategies to two representatives of DFID, which sparked further questions and discussion.

Not known.
Year(s) Of Engagement Activity 2012
 
Description Presentation to Elsoms Seeds, Spalding, UK 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Professional Practitioners
Results and Impact I was invited to present my work on speed breeding and resistance gene cloning to Elsoms Seeds, Lincolnshire, and to troubleshoot their LED lighting in the glasshouse. This has since resulted in further interactions and dicussions.
Year(s) Of Engagement Activity 2017
 
Description Presentation to Joe Cerrell & James Birch, BMGF 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Supporters
Results and Impact Joe Cerrell & James Birch frin the Bill and Melinda Gates Foundation came to visit JIC and I was called upon to present my work on wheat stem rust and the solution we are working on to try to mitigate this problem for small holder farmers in Africa.
Year(s) Of Engagement Activity 2016
 
Description Presentation to KWS, Einbech, Germany 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact I was invited to present my work on speed cloning and speed breeding at KWS, Einbech, Germany. KWS are currently considering how our enabling technologies could be integrated in one of their own crop breeding programmes and how we may collaborate to achieve this.
Year(s) Of Engagement Activity 2018
 
Description Presentation to Owen Patterson, MP (June 2014) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Policymakers/politicians
Results and Impact I gave a presentation on wheat stem rust and potential GM solutions to Owen Patterson.

This stimulated further discussion and questions, and subsequent requests for further information.
Year(s) Of Engagement Activity 2014
 
Description Presentation to delegation from Elsoms Seeds, Lincolnshire, UK 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Industry/Business
Results and Impact I invited 8 delegates from Elsoms Seeds to visit JIC for some bespoke presentations from my group and a colleauge, and for an inspection of our speed breeding facilities. This has helped cement a relationship that now stretches back more than one year.
Year(s) Of Engagement Activity 2018
 
Description Putting up resistance - The Scientist (June 2014) 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact I was interviewed by a journalist from "The Scientist" about my work on wheat stem rust and GM crops.

This article stimulated a lot of responses from colleagues, friends and family.
Year(s) Of Engagement Activity 2014
URL http://www.the-scientist.com/?articles.view/articleNo/40085/title/Putting-Up-Resistance/
 
Description Salt-tolerant rice "Could aid Japan's Tsunami farmers" - BBC News Norfolk 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Media (as a channel to the public)
Results and Impact The JIC/TSL Communications Team released a press release which resulted in an interview by a journalist from the BBC which in turn lead to an article in the BBC website.

Not known.
Year(s) Of Engagement Activity 2012
URL http://www.bbc.co.uk/news/uk-england-norfolk-16699426
 
Description Science Art and Writing, Norfolk Primary School, March 2012 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact 30 pupils attended a one day activity on science, art and writing on the theme of biotechnology in crops.

The School teachers and pupils enjoyed the day and were very engaged, asking lots of questions.
Year(s) Of Engagement Activity 2012
URL https://www.youtube.com/watch?v=OKFPlBUGiLg&feature=email
 
Description Science Collaboration Agritech, Aquaculture and Food Safety Forum, Muscat, Sultanate of Oman 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I was invited to present at the Science Collaboration Agritech, Aquaculture and Food Safety Forum, Muscat, Sultanate of Oman.
Year(s) Of Engagement Activity 2018
 
Description Standing up to stem rust - Financial Times 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Media (as a channel to the public)
Results and Impact I was interviewed by a reporter from the Financial Times and this resulted in a short article a few days later.

I received lots of feedback from colleagues, friends and family.
Year(s) Of Engagement Activity 2011
URL http://www.ft.com/cms/s/2/c52a3d8e-100b-11e1-a468-00144feabdc0.html#axzz36xnLjKYx
 
Description Teleconference discussion with Syngenta 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact Participated in a teleconference with Syngenta with participation from China, UK and USA. Follow-on discussions are ongoing.
Year(s) Of Engagement Activity 2018
 
Description The Sainsbury Laboratory Annual Retreat 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact I presented my yearly update to The Sainsbury Laboratory as part of my Adjunct Affiliation with this institution.
Year(s) Of Engagement Activity 2018
 
Description The Sainsbury Laboratory Retreat 2017, UK 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact I was invited to present my work on R gene cloning and speed breeding at The Sainsbury Laboratory 2017 retreat. This sparked questions and discussions and has since contributed to further collaboration with colleagues in TSL.
Year(s) Of Engagement Activity 2017
 
Description Two invited presentations at Next-generation wheat breeders workshop, University of Saskatchewan, Canada 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Myself and my postdoc, Sanu Arora, were invited to present on our enabling technology for rapid cloning of disease resistance genes by association genetics. This sparked a lot of interest and led to an invitation to present again at the International Wheat Congress, Saskatchewan, Canada, the following week.
Year(s) Of Engagement Activity 2019
URL https://2019iwc.ca/pre-conference-workshop/
 
Description Wheat molecular genomics workshop in Cairo, Egypt 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact In collaboration with Dr Ahmed Elkot of the Agricultural Research Centre (ARC) in Cairo, Egypt, we organised a workshop for wheat researchers and breeders. Approximately 50 scientists attended including the Minister of Agriculture and the President of the ARC (which employs ~60,000 staff). The event was a great success and covered in the national media in Egypt. The ARC staff were very honoured that the Minister of Agriculture attended.
Year(s) Of Engagement Activity 2019
URL https://www.facebook.com/526865257491839/posts/1170288249816200/
 
Description Workshop at BecA-ILRI, Kenya on Molecular Breeding in Small Grain Cereals 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I co-organised and taught a short 4-day course on molecular breeding in small grain cereals (wheat, barley and teff) for African scientist and breeders. Participants came from Ethiopia (~12), Kenya, Uganda, Tanzania, Madagascar, Nigeria and Burundi. The course was well received, led to requests for additional information, request to repeat the course the following year, and the possibility of a new strategically interesting collaboration with young career wheat scientist from Ethiopia.
Year(s) Of Engagement Activity 2017
URL http://hpc.ilri.cgiar.org/beca/training/jic_beca_2016/index.html
 
Description Workshop on Scientific Softskills, Punjab Agricultural University, Ludhiana, India 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact I organised and presented, with colleagues, a workshop on scientific soft skills to early career scientists primarily from India, but also from the UK, Tunisia and Iran.
Year(s) Of Engagement Activity 2017
 
Description Workshop on scientific communication for early career scientists in Pwani, Kenya 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Along with my colleague Prof. Cristobal Uauy, I was invited to give a two-day course on scientifict communication to early career scientists from across Sub Saharan Africa as part of the ACACIA AfriPlantSciences course. The course involved practical sessions on scientific writing, oral presentation, CV + cover letter, job interview, and applying for a travel award. The participants responded very positively to the course.
Year(s) Of Engagement Activity 2019
URL https://acaciaafrica.org/afriplantsci/