Development of complementary strategies for the analysis of dietary bioactives in foods and biofluids

Lead Research Organisation: University of East Anglia
Department Name: Norwich Medical School

Abstract

Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.

Publications

10 25 50
 
Description Outputs to date include preliminary data from pilot studies which suggest that storage of juice concentrates does not result in degradation of anthocyanins but rather the formation of larger molecular weight compounds of unknown structure. We are presently analyzing juices which have been stored under simulated 'house-hold usage' conditions for suspected products of condensation and polymerization.
Exploitation Route The findings from this project will provide evidence for the potential benefits to public health of consuming processed fruit products and in particular beverages; which is important given the current low intake of raw/fresh fruits and vegetables in the diet. The impact of processing and storage on food bioactivity, as explored in this proposal, will identify dietary constituents that may be exploited as novel bioactives with potential functional-food and agricultural implications.

Providing proof of the health benefits of anthocyanin-rich juices also holds industrial and agricultural benefit as anthocyanin levels in food crops could be easily modified using pre- and post-harvest manipulation or plant breeding or genetic approaches to produced products of increased bioactivity. As juices are likely to be the major delivery vehicle of anthocyanins to the consumer, this work holds particular relevance to the juice industry and consumers. The project is currently providing valuable training and knowledge transfer between research groups at the University of East Anglia (UEA; UK) and the University of California Davis (UCD; USA). Research staff (Primary Investigators, PhD Students and Research Associates) at the UEA are providing training regarding the extraction and analysis of anthocyanin metabolites in biological samples (urine, serum, faeces) while research staff at UCD are providing training on the extraction and identification of flavonoid polymers in juices, wines and faecal samples. This project will provide valuable training required for the analysis of existing clinical samples and the development of future project grants between the two Universities.

This project is of particular importance to phytochemcial researchers as the study designs explored will define experimental approaches for defining the active compounds responsible for the health benefits of flavonoid-rich foods. Ultimately, this project will provide evidence on the structure of novel compounds found in stored juices and there bioactivity. This evidence will be essential to the design of future dietary intervention studies with the goal of determining the effects of juice consumption on chronic disease outcomes.
Sectors Agriculture, Food and Drink,Healthcare,Manufacturing, including Industrial Biotechology,Retail

 
Description Norwich Rsearch Park Enterprise and Engagement award, 2014
Amount £3,000 (GBP)
Organisation Norwich Research Park 
Sector Private
Country United Kingdom
Start 10/2014 
End 10/2015
 
Description Collaboration with AM, UCD, 2012 
Organisation University of California, Davis
Department Department of Food Chemistry
Country United States 
Sector Academic/University 
PI Contribution During a visit as part of the present IPA, collaboration with the Department of Food Chemistry was formed to foster future collaboration on the analysis of glutathione conjugates of flavonoids
Collaborator Contribution Future development of potential collaboration
Impact No outputs
Start Year 2012
 
Description Collaboration with F.S. of University of California Davis, 2013 
Organisation University of California, Davis
Department Department of Nutrition
Country United States 
Sector Academic/University 
PI Contribution During a visit as part of the present IPA, collaboration with the Department of Nutrition was formed to foster future collaboration on the analysis of flavonoids in clinical samples from an on-going clinical intervention at Davis.
Collaborator Contribution Potential future clinical collaboration
Impact None as of yet
Start Year 2013
 
Description Collaboration with Plant Science Group, Cranfield University, Cranfield, 3013 
Organisation Cranfield University
Department Plant Science Group
Country United Kingdom 
Sector Academic/University 
PI Contribution Collaboration established through attendance at BBSRC DRINC dissemination events led to meeting with staff in the department of Health at Cranfield University which lead to a formal collaboration to explore flavonoid polymer formation in juices with the present IPA collaborators at UCD
Collaborator Contribution development of BBSRC project Grant
Impact development of BBSRC project grant
Start Year 2013
 
Description Collaborative engagement meeting for programme development with the Dpt of Nutriton at UCD, 2013 
Organisation University of California, Davis
Country United States 
Sector Academic/University 
PI Contribution Met to help with development of a method at UCD (in the Dpt Nutrition) for analysing anthocyanins in clinical samples and to arrange a PhD student Exchange to send a PhD student (MS) for two weeks to transfer methods to University of California for isolation and identification of anthocyanins, including SPE and MS analysis of biological samples.
Collaborator Contribution Potential for future shared publication
Impact none at this time
Start Year 2013
 
Description Methods Transfer Meeting, F.S. 2014 
Organisation University of California, Davis
Country United States 
Sector Academic/University 
PI Contribution Helping develop a method for anthocyanin metabolite analysis of clinical samples at UCD
Collaborator Contribution Potential for future clinical collaboration
Impact NA
Start Year 2012
 
Description PepsiCo Collaboarative Meeting at Cranfield University, Oct 2014 
Organisation PepsiCo
Country United States 
Sector Private 
PI Contribution Proposal outlined to members of PepsiCo research and nutrition divisions exploring development of processing technology
Collaborator Contribution Met for a 4 hour meeting to explore future engagement and TSB application
Impact None as of yet
Start Year 2014
 
Description PhD Research Student Placement (JC) between UCD and UEA, Sept 1-Oct 30 
Organisation University of California, Davis
Department Department of Viticulture and Enology
Country United States 
Sector Academic/University 
PI Contribution We provided expertise in Q-Trap method development and optimisation and training to the UCD student to take back to UCD and provide support for JCs PhD thesis
Collaborator Contribution Development of methodologies for analysis of cocoa, grape and blackcurrant polymers in solution, transferring previous MS methodologies established at UCD to UEA QTrap. Subsequent work to be progressed in collaborators lab in Germany.
Impact Continuing method development and validation
Start Year 2015
 
Description PhD student Exchange (RdH) between UEA and UCD, 2013 
Organisation University of California, Davis
Department Food Science and Technology
Country United States 
Sector Academic/University 
PI Contribution PhD student Exchange (RdF) between UEA and UCD. PhD student spent two weeks in labs at the University of California transferring methods for isolation and identification of anthocyanins, including SPE and MS analysis of biological samples.
Collaborator Contribution PhD student worked with the department of Viticulture to establish methods for analysis of pyranoanthocyanins in blackcurrant juice to implement in the UK.
Impact methods transfer between research groups
Start Year 2013
 
Description Project Development meeting, UCD, 2013 
Organisation University of California, Davis
Country United States 
Sector Academic/University 
PI Contribution PI travelled to UCD to establish future funding opportunities and to develop a project outline to explore the biological activity of flavonoid polymers. A BBSRC-DRINC project grant outline was progressed and parallel funding streams in the US were explored.
Collaborator Contribution contribution to project development
Impact programme development
Start Year 2013
 
Description Project outline meeting, Waterhouse Lab, UCD, 2012 
Organisation University of California, Davis
Country United States 
Sector Academic/University 
PI Contribution PI travelled to UCD to establish future project goals and identify target juices and wines for polymer method development. Methods from UEA in the analysis of anthocyanin metabolites in human clinical samples were exchanged with PhD students at UCD to implement in their current research projects. Researchers at UCD also provided guidance in the extraction and identification of flavonoid polymers in wines and rat faecal samples.
Collaborator Contribution Expertise shared in flavonoid polymer extraction and analysis
Impact further programme and method development, establishing studentship exchange
Start Year 2012
 
Description Project outline meeting, Waterhouse Lab, UCD, 2014 
Organisation University of California, Davis
Country United States 
Sector Academic/University 
PI Contribution PI travelled to UCD to establish future project goals and plans for student exchange placement
Collaborator Contribution Establish future collaboration and student exchange
Impact established student exchange programme to transfer methods between UEA and UCD
Start Year 2010
 
Description Student Tranfer placemetn between UCD and UEA, April 2015 
Organisation University of California, Davis
Country United States 
Sector Academic/University 
PI Contribution We provided training and expertise in Q-trap method development, optimisation and analysis of small molecules to University of California Davis (UCD) PhD student (J. Cave), and access to extraction and analytical chromatography platforms (not available at UCD) for identifying wine tannins in extracts from aged Californian wines, which were imported as part of the collaboration.
Collaborator Contribution PhD student J. Cave travelled to the University of East Anglia (from University of California Davis; UCD) to transfer methods developed at the UCD for the extraction and analysis of flavonoid polymers from wines, to researchers in the departments of Nutrition to aid in their future work exploring the dynamic equilibrium of bioactives in functional beverages, which is being developed for a BBSRC-DRINC grant application. Researchers at UCD also provided training to UEA PhD student M. Smith for the extraction and identification of flavonoid polymers.
Impact NA
Start Year 2012
 
Description Student Tranfer placemetn between UEA and UCD, 2014 
Organisation University of California, Davis
Country United States 
Sector Academic/University 
PI Contribution PhD student M.S. travelled to UCD for two weeks lab experience
Collaborator Contribution expertise and training
Impact lab methods transfer for anthocyanin polymer extraction and analysis
Start Year 2013
 
Description Tour of the MARS research laboratories, Dpt Nutrition, University of California Davis, 2014 
Organisation University of California, Davis
Country United States 
Sector Academic/University 
PI Contribution expertise and intellectual input shared, including discussion regarding future studentship exchange programme and collaborative research
Collaborator Contribution expertise and intellectual input shared, including discussion regarding future studentship exchange programme and collaborative research
Impact Invited to give an oral presentation to the department and meet with lab manager
Start Year 2014
 
Description Invited Lecture to the Norwich Medical School and Norwich Research Park, University of East Anglia, UK, 2014 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact 20 PhD students, research associates and academics attending a lecture 'Wine quality: insights in wine oxidation chemistry and California wine' presented by collaborator Andrew Waterhouse

no direct impact
Year(s) Of Engagement Activity 2014
 
Description Invited Seminar to John Innes Centre, 17 April, 2015 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact Visiting UCD PhD student J. Cave presented 'Wine Pigmented Tannin: Compositional Elucidation by a Complimentary Suite of Mass Spectrometric Techniques' for an Invited Seminar to the John Innes Centre Metabolic Biology Departmental Seminar Series; Norwich Research Park, G34/35 JIC Conference Centre, 17 April, 2015. Talk sparked question, discussion and led to the identification of a number of potential new collaborations.


Talk sparked question, discussion and led to the identification of a number of potential new collaborations.
Year(s) Of Engagement Activity 2015
 
Description Invited Seminar to MARS research and the department of Nutrition, University of California, 2014 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact 35 Academics, PhD students and Research associates attended the seminar 'Establishing the effectiveness of dietary flavonoids in improving health, through understanding the structural activity relationships of their metabolites' Presented to the Department of Nutrition & Mars Research Division Sept 25, 2014, UC Davis, CA.


no notable impacts
Year(s) Of Engagement Activity 2014
 
Description Invited Seminar to the Departments of Food Science & Technology, Nutrition, and Vitaculture University of California, 2013 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact 35 Academics, Research Associates and PhD students attended an Invited seminar 'Analytical methods for isolation and identification of anthocyanin metabolites' provided by PhD student RdF as part of a student exchange

Aid in methods exchange between UCD and UEA
Year(s) Of Engagement Activity 2013
 
Description Invited Seminar to the Departments of Food Science & Technology, Nutrition, and Vitaculture, University of California, 2012 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact 30-50 academics and students from the departments of Viticulture, Food Science, Nutrition and Agriculture, from the University of California Davis, attended the Invited Lecture: Oral presentation - Exploring the Impact of Processing on Anthocyanin Bioactivity and introducing a proposed collaborative project exploring the biological effect of consuming pyranoanthocyanins in juices and wines Oral presentation

Established collaborators for future proposals
Year(s) Of Engagement Activity 2012
 
Description Invited Seminar to the Departments of Food Science & Nutrition, University of California Davis, 2013 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact 30+ PhD students and academics attended a Seminar focused on our findings regarding the utility of Anthocyanin metabolites in cardiovascular disease prevention and management and introducing the potential of our collaborative project exploring the biological effect of consuming pyranoanthocyanins in juices and wines. Oral Presentation - 'Exploring the utility of Anthocyanin metabolites in cardiovascular disease prevention and management'

promoted the development of further collaboration with the intent of designing future research proposals underway
Year(s) Of Engagement Activity 2013
 
Description Invited Seminar to the Departments of Food Science, Nutrition, and Vitaculture, University of California Davis, 2013 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact 40+ PGR students attended an Invited Seminar to the Departments of Food Science & Technology, Nutrition, and Vitaculture 'exploring The metabolic fate of anthocyanins in humans and further collaborative work describing methods of analysis of anthocyanin metabolites in clinical samples' Oral presentation

no actual impacts realised to date
Year(s) Of Engagement Activity 2013
 
Description Workshop to the Department of Nutrition, bioanalytical group, University of East Anglia, UK, 2014 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Postgraduate students
Results and Impact 6 PhD students, 2 Research Associates and 2 PIs attended a workshop 'tannin and pyranoanthocyanidin chemistry: extraction, identification and quantification' presented by UCD partner Andrew Waterhouse.

led to development of method
Year(s) Of Engagement Activity 2014