Other Countries Partnering Award [Australia] Understanding common mechanisms of pathogenicity in Mycoplasma species.

Lead Research Organisation: Royal Veterinary College
Department Name: Pathology and Pathogen Biology

Abstract

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Description This grant began in April 2014. We have engaged very positively with UTS in Australia. We have hosted a senior PhD student for 3 months enabling him to learn new techniques and take them back to Australia. We have applied for funding to the Australian government (ANR as collaborator). We have published joint resultsfor the 3rd Prato Conference on the Pathogenesis of Bacterial Diseases of Animals in Prato, Italy, October 2014. This won first prize. We have also hosted Prof Steve Djordevic at RVC for a research seminar and held useful discussions on future collaboration and research opportunities. These have included generating results of in vivo testing of key mutants of M. hyopneumoniae, and the transfer of our techniques and our entire arrayed mutant library to Sydney to facilitate their research.

We have also engaged with Prof Glenn Browning in Melbourne and held discussions on ways to improve our understanding of common aspects of pathogenicity in animal Mycoplasma species. We have shared our technical expertise with these researchers and also with others in Brazil and in Europe.
Exploitation Route Proteomic analysis of M hyo mutants at UTS.
Sharing of results of in vivo studies of selected mutants of interest to both parties.
Improved culture of M .hyopneumoniae.
Experiumental work with mutants to determine common and specific mechanisms of pathogenicity in Mycoplasma species.
Sectors Agriculture, Food and Drink,Healthcare,Pharmaceuticals and Medical Biotechnology,Transport

 
Description UTS Sydney 
Organisation University of Technology Sydney
Country Australia 
Sector Academic/University 
PI Contribution Hosting of a PhD student in the lab at RVC for 3 months. Transfer of techniques for culture and mutagenesis of Mycoplasma hyopneumoniae. Identification of genes of M. hyopneumoniae as contributing to aspects of pathogenicity and biofilm formation. Transfer of gridded (ordered) library of 5000 transposon mutants (3000 from RVC lab) to UTS Sydney. Contribution, as co-applicant, on a grant proposal to the Australian Research Council 2014 and 2015. Discussions of aberrant (non-conventional) mycoplasmal respiratory disease (fibrinous pleuritis) observed in pigs and cattle.
Collaborator Contribution Seminar by Steve Djordjevic at RVC. Inclusion of ANR and GAM on a poster at 3rd Prato conference on Pathogenicity of Bacteria in Animal Disease (October 2014). Advice on microscopic techniques and stains for Alanah Deeney, PhD student by Ben Raymond. Meetings in Sydney and Melbourne to discuss current understanding of Mycoplasma pathogenicity and future research direction. Discussion of possible visit to UTS by junior staff and students particularly for their microscope expertise and facility.
Impact Poster at 3rd Prato Conference.: Raymond, B., Rohde, M., Padula, M., Maglennon, G.A. Rycroft, A.N. & Djordjevic, S. (2014). Proteins involved in the adherence of Mycoplasma hyopneumoniae to abiotic surfaces and porcine monolayers which may also play a role in biofilm formation. Proceedings of the 2nd Prato Conference on the Pathogenesis of Bacterial Diseases of Animals. Prato, Italy, October 2014. abs #36.
Start Year 2014
 
Description Round table discussions - Melbourne 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Detailed discussions on Mycoplasma pathogenesis and experiences of animal models with participants at University of Melbourne Vet School.
Year(s) Of Engagement Activity 2016
 
Description SD Presentation 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact A talk by SD, followed by extended discussion, on theories for the ability of Mycoplasma hyopneumoniae to cause host tissue damage.
Year(s) Of Engagement Activity 2015
 
Description Seminar at UTS 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Seminar to discuss research progress in Mycoplasma research held at UTS Sydney.
Year(s) Of Engagement Activity 2015
 
Description Workshop UTS Sydney 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Staff and post-graduates attended a Workshop we organised at UTS Sydney to discuss transfer of materials and strains and to assess the current understanding of pathogenicity of Mycoplasma species.
Year(s) Of Engagement Activity 2016