Distinguishing between beneficial and detrimental effects of FoxO in Drosophila ageing: interactions between FoxO and ETS transcription factors.

Lead Research Organisation: University College London
Department Name: Genetics Evolution and Environment

Abstract

The proportion of aged individuals is increasing in our society. For many, ageing means deteriorating health and wellbeing, loss of physical and mental abilities and increased susceptibility to numerous diseases. We are thus exposed to ever-increasing human and socioeconomic costs of ageing that need to be urgently addressed. For centuries we have understood ageing as an immutable fact of life. It came as a big surprise when we discovered that certain interventions can actually improve health in old age. Scientific effort is now invested into understanding how these interventions act and harnessing that knowledge to improve human health and wellbeing into old age.
Down-regulation of the signals that indicate the presence of nutrients, such as the well-known hormone, insulin, improve life-long health in organisms as different as worms, fruit flies and mice. There is a growing awareness that similar interventions may work in humans. However, down-regulation of these pivotal nutrient sensing mechanisms often has major detrimental effects precluding them from human use. For example, inability to produce or respond to insulin results in diabetes. We now need to understand how to capture the benefits of these interventions without causing harm.
Numerous interventions that extend healthy lifespan act by changing how the genetic information, carried in the organism's DNA, is used. They often specifically alter the first step in the expression of genetic information where the DNA code is transcribed into an RNA molecule. The regulation of transcription is performed by transcription factors and one such factor, called FoxO, has the ability to robustly improve life-long health and well being in all organisms examined. Indeed, certain variants of the gene encoding FoxO are also present in exceptionally long-lived humans, contributing to their longevity. However, similar to other anti-ageing interventions, FoxO activation can be detrimental. Hence, FoxO provides a good model to establish the molecular events that differentiate the two opposing outcomes.
I have recently discovered that the detrimental effects of FoxO are dependent on two other transcription factors called Pnt and Aop in the fruit fly. FoxO activated alone is beneficial but becomes detrimental if Pnt is activated as the same time. Pnt and Aop are engaged in a tug of war and Aop fosters beneficial effects of FoxO by counter-acting Pnt. Hence, health in old age is not solely determined by FoxO activity but is a matter negotiated between FoxO and Pnt/Aop. Understanding the intricacies of FoxO and Pnt/Aop exchange will define the molecular events that distinguish the good from the bad outcomes of FoxO activation.
I propose to use genetic and biochemical approaches to query how Pnt swings the effects of FoxO activation from good to bad. I will firstly examine the physical and functional interactions between FoxO and Pnt/Aop on the parts of DNA from which transcription is regulated, both in a test tube and in an intact cell. Secondly, I will determine how Pnt alters the pattern of gene regulation by FoxO in the relevant organs of the adult fruit fly. This will triage the genes that underlie the beneficial from those associated with the detrimental effects of FoxO activation. Pnt and Aop are members of a gene family - a set of genes that are related and likely perform similar functions. I will examine whether all members of this family show similar interactions with FoxO in a panel of fly organs, to determine whether the findings are more broadly applicable.
The study I am proposing is multifaceted, and the different aspect will converge to give us a clear understanding of the events, at a molecular scale, that differentiate the beneficial effects of FoxO activation from the potential detrimental outcomes. This knowledge will be important in devising strategies that can be used to improve human health and well being in old age, without unwanted, detrimental consequences.

Technical Summary

Ageing is of growing medical, social and economic importance. Recent work in biogerontology has revealed mechanisms that could be used to improve human health and wellbeing in old age. However, these interventions also have a demonstrated potential to be detrimental. It is now imperative to understand how to harness the beneficial effects without the associated risks.
Key, evolutionarily conserved determinants of animal lifespan are the transcription factors (TFs) of the Forkhead Box O (FoxO) family. Variation in FoxO3A locus is robustly correlated with human longevity, and activation of FoxO members counteracts ageing in animal models.
The physiological effects of FoxO activation are context dependent, with both beneficial and detrimental outcomes observed. I have recently shown that the balance between the opposing effects of FoxO is dictated by its interaction with two evolutionarily conserved TFs of the E-twenty six (ETS) family in Drosophila. This interaction now provides an avenue to elucidate mechanisms differentiating the beneficial from detrimental effects of FoxO.
I propose to study this interaction at several levels. Guided by my recent data, I will examine in detail the physical and functional interactions between the TFs on gene-regulatory regions, in vitro and in vivo. In an in vivo, lifespan-relevant system, I will determine how the tissue-specific, genome-wide response to FoxO is altered by the two ETS TFs, simultaneously triaging the anti-ageing processes regulated by FoxO. Lastly, I will extend the observations to different ETS factors and different tissues and organs of the fruit fly.
As well as improving our knowledge of how transcriptional regulation underlies complex adult traits, the study will generate an integrated picture of how FoxO and ETS factors together determine animal's health and well being throughout life. This will allow us to harness the beneficial effects of FoxO activation while avoiding detrimental outcomes.

Planned Impact

Potential beneficiaries of this research, in short and long-term, include:

1) Public care and healthcare services.
A substantial and ever-increasing amount of care efforts are targeted at older people. In the long-term, this basic research has the potential to result in treatments that reduce the occurrence of ageing-related health and fitness issues and hence will reduce the overall cost of care, including healthcare, in today's society, increasing effectiveness of a public service. There is a possibility that an anti-ageing intervention or drug may allow for increased health and wellbeing of the aged and prevent multiple, detrimental manifestations of ageing, hence further decreasing treatment cost. New treatments/interventions increasing the health and wellbeing at later ages, which may ultimately result from this research, will also improve the quality of the care system.

2) Older people.
Older people represent an ever-increasing portion of our society and often face immense personal costs due to ageing-related loss of function, decreased overall health and wellbeing, and increased occurrence of ageing-related conditions. The final aim of this research is to provide a basis for development of treatment for age-related conditions. In the long-term, this will result in tangible benefits in terms of increased quality of life, health, wellbeing and creativity and decrease in emotional and physical suffering for older people. This effect will not just be national but international. The research will also increase the awareness of others to the problems of ageing.

3) Economy.
This project will have an impact on the UK economy in several ways, both in short and long-term. Firstly, in the short-term, the project will create one new job as well as provide training thus creating a highly skilled worker for e.g. the pharmaceutical industry. In the long-term, reduction in public care costs will liberate funds for investment into the economy. New interventions for treatment of ageing-related disease that will be developed as a result of research initiated in this project will benefit the pharmaceutical industry and hence the economy. Treatments that extend health into old age will provide more work force aiding the economy. The project will raise the research profile of the UK leading to more investments by the pharmaceutical industry. All of these will have an effect of increasing the economic performance, competitiveness and reputation of the UK.

4) Government policy.
This project will have an impact on the UK government and its policy. In short term, this research will further establish if ageing is a viable target for treatment of age-related conditions, and if increased health and wellbeing can be achieved in older age, thus informing government policy on the feasibility of this approach and whether further funding in this area of scientific enquiry is required and justified. In the long-term, the effects on the health of older people may inform government policy in numerous important areas such as healthcare and pensions.

Publications

10 25 50
 
Description Ageing populations pose one of the main public health crises of our time. Reprogramming gene expression by altering the activities of sequence-specific transcription factors (TF) can ameliorate deleterious effects of age. Here we explore how a circuit of TFs coordinates pro-longevity transcriptional outcomes, which reveals a multi-tissue and multi-species role for an entire protein family: the E-twenty-six (ETS) TFs. In Drosophila, reduced insulin/IGF signalling (IIS) extends lifespan by coordinating activation of Aop, an ETS transcriptional repressor, and Foxo, a Forkhead transcriptional activator. Aop and Foxo bind the same genomic loci, and we show that, individually, they effect similar transcriptional programmes in vivo. In combination, Aop can both moderate or synergise with Foxo, dependent on promoter context. Moreover, Foxo and Aop oppose the activities of Pnt, an ETS transcriptional activator, effecting a transcriptomic programme that correlates lifespan outcomes. Directly limiting Pnt extended lifespan, suggesting this is how Aop and Foxo promote longevity. The lifespan-limiting role of Pnt appears to be balanced by a requirement for metabolic regulation in young flies, in which the Aop-Pnt-Foxo circuit determines nutrient storage, and Pnt regulates lipolysis and responses to nutrient stress. Molecular functions are conserved amongst ETS TFs, suggesting others may also affect ageing. We show that Ets21C limits lifespan, functioning in the same genetic network as Foxo and IIS. Other ETS TFs appear to play roles in fly ageing in multiple contexts, since inhibiting the majority of the family in intestine, adipose or neurons extended lifespan. We expand the repertoire of lifespan-limiting ETS TFs in C. elegans, confirming their conserved function in ageing. Altogether this study reveals that roles of ETS TFs in physiology and lifespan are conserved throughout the family, both within and between species.

The manuscript describing these findings is currently under review at PLoS Genetics.
Exploitation Route These finding will be useful to scientists working on the basic biology of ageing and age-related diseases as well as to those working on gene regulation. They can be pursued toward treatments to ensure life-long health.
Sectors Communities and Social Services/Policy,Healthcare,Pharmaceuticals and Medical Biotechnology

 
Description 1) We seized a scientific opportunity to characterise the effect of early life nutrition on FoxO activity and consequently on lifespan. These findings were reported in a scientific journal (Dobson et al. Cell Reports 2017). These findings may have an important impact on informing the public about the consequences of poor dietary choices. To insure this impact is achieved we mobilised the UCL media office and ensured distribution of our findings to the general public in a easily accessible form. We achieved broad and international media coverage, including press coverage in the "Sun" (11 Jan 2017) and the following coverage online: http://online.isentialink.com/theaustralian.com.au/2017/01/11/3b295c49-689c-4393-a7fa-c4da1de6e5f4.html http://online.isentialink.com/heraldsun.com.au/2017/01/11/54e5f4dd-f747-4b91-8422-38b8add0bc2c.html https://www.oliveoiltimes.com/olive-oil-health-news/flies-fed-sugar-rich-diets-die-faster/55026?utm_content=buffer80887&utm_medium=social&utm_source=facebook.com&utm_campaign=buffer https://www.ucl.ac.uk/news/news-articles/0117/100117-high-sugar-diet-in-flies https://twitter.com/uclnews/status/819143086572007424 https://twitter.com/wellcometrust/status/819189627906965505 https://twitter.com/IHAatUCL/status/819133380986109953 https://twitter.com/a_j_dobson/status/818861562815516675 https://twitter.com/mattpiperlab/status/819303661524250624 2) The post-doctoral scientist on this award assisted in completing the research for an important publication (Filer et al Nature 2017). The findings may have an strong impact on our understanding of how ageing can be modulated and on how potential anti-ageing drugs, such as rapamycin, act. To insure wide spread of the findings to the scientific and wider community we released a press release through the UCL media office. We achieved broad, international online coverage by the scientific press such as: https://www.myscience.org/news/2017/lifespan_prolonged_by_inhibiting_common_enzyme-2017-UCL http://www.spacedaily.com/reports/Researchers_prolong_life_by_curbing_common_enzyme_999.html https://phys.org/news/2017-11-lifespan-prolonged-inhibiting-common-enzyme.html The publication of the paper attracted 296 tweets and re-tweets to date. 3) The post-doctoral scientist on this award has now completed his main project. We have released the findings in a preprint: https://www.biorxiv.org/content/10.1101/438879v1 The pre-print has been accessed over 180 times to date. We have also generated extensive expression datasets that will be uploaded to public databases and available upon the publication of the results. The results are currently in review in PLoS Genetics. 4) Both the post-doc and the PI have extensively communicated to the public in forms of public talks to a range of different audiences.
First Year Of Impact 2017
Sector Education,Healthcare,Other
Impact Types Cultural,Societal

 
Description DRESDEN Fellowship
Amount € 13,800 (EUR)
Organisation Technical University of Dresden 
Sector Academic/University
Country Germany
Start 05/2019 
End 10/2019
 
Description Programming of lifespan by insulin/IGF-like signalling in Drosophila.
Amount £532,221 (GBP)
Funding ID BB/R014507/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 06/2018 
End 05/2021
 
Description Sea and Currents Fund, UCL Global Engagements
Amount £1,500 (GBP)
Organisation University College London 
Sector Academic/University
Country United Kingdom
Start 02/2017 
End 03/2017
 
Title Drosophila gut transcriptome in response to RNA Polymerase III inhibition 
Description We examined the changes in the transcriptome observed upon RNAi against the largest subunit of RNA polymerase III in the fly gut. 
Type Of Material Database/Collection of data 
Year Produced 2017 
Provided To Others? Yes  
Impact It's too early to tell. 
URL https://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-5252/
 
Title Effects of reduced IIS and Fkh on gut transcriptome in Drosophila 
Description RNA-sequencing in gut tissue on controls and reduced insulin signalling upon control RNAi and FKH RNAi ). 
Type Of Material Database/Collection of data 
Year Produced 2017 
Provided To Others? Yes  
Impact It's too early to tell. 
URL https://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-6056/
 
Title Survey of the Drosophila transcriptome in response to excess dietary sugar 
Description Data from an RNA-Seq experiment profiling change in transcripts as flies experience a transient increase in dietary sugar content. Data were deposited in ArrayExpress (E-MTAB-4766) 
Type Of Material Database/Collection of data 
Year Produced 2017 
Provided To Others? Yes  
Impact This data can be freely accessed for further analysis by other groups. 
URL https://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4766/
 
Description Conservation of FoxO function from flies to worms 
Organisation University College London
Department Department of Physics & Astronomy
Country United Kingdom 
Sector Academic/University 
PI Contribution We collaborated with Prof. David Gems on understanding the conservation of FoxO function betewwwn flies and worms. Our primary findings in flies (reported in Dobson et al. Cell Reports 2017) were validated in worms.
Collaborator Contribution Prof. Gems's groups contributed research findings that showed the functions we found for FoxO are also observed in the worm.
Impact The collaboration resulted in a publication (Dobson et al. Cell Reports, 2017). The work was widely cover in print and online media.
Start Year 2016
 
Description Distinguishing mitochondrial and nuclear genetic variation in dietary impacts on lifelong health 
Organisation Technical University of Dresden
Country Germany 
Sector Academic/University 
PI Contribution I conceived the project and wrote the grant proposal.
Collaborator Contribution My partners will provide salary (ie. the value of the grant), laboratory facilities, consumables, and access to Drosphila genetic resources (in-lab selection lines)
Impact I will visit TU Dresden for this 6-month fellowship.
Start Year 2019
 
Description Effects of diet on FoxO activity. 
Organisation Monash University
Department Anatomy and Human Biology
Country Australia 
Sector Academic/University 
PI Contribution We collaborated with Dr Mtthew Piper (an expert in Drosophila nutrition) in order to understand the regulation of FoxO by the diet.
Collaborator Contribution Dr M. Piper provided expertise and experimental support to our work on how nutriotion regulated FoxO activity.
Impact This work resulted in a publications (Dobson et al. Cell Reports, 2017). The work was covered by press and online media.
Start Year 2016
 
Description Evolutionary conservation 
Organisation University of Kent
Country United Kingdom 
Sector Academic/University 
PI Contribution Examination of the evolutionary conservation of longevity effects.
Collaborator Contribution Examination of the evolutionary conservation of longevity effects.
Impact Filer et al Nature 2017 publication
Start Year 2016
 
Description Microbial thiamine provision and lifelong health 
Organisation Cornell University
Country United States 
Sector Academic/University 
PI Contribution I remotely co-supervised a PhD student, aiding in manuscript preparation, data analysis, and project conceptualisation.
Collaborator Contribution The collaborator provided funds, laboratory space and studentship for the PhD student.
Impact Publication: The Drosophila melanogaster gut microbiota provisions thiamine to its host. MBio. 2018 Mar 6;9(2) PMID 29511074
Start Year 2018
 
Description "Lost in Thought" - Science Museum Lates public open evening 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Public outreach activities, discussing the impact of ageing on dementia, and presenting interactive exhibits showing use of fruit flies in associated research.
Year(s) Of Engagement Activity 2016
URL https://www.ucl.ac.uk/pals/research/experimental-psychology/event/lost-in-thought-science-museum-lat...
 
Description "Pint of Science" festival talk. 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact A public talk designed to engage the general public with science in their local area. Part of an international programme of parallel events. Attendees recieved the talk well and discussed its possible implications for human health.
Year(s) Of Engagement Activity 2017
 
Description British Society for Research on Ageing - selected talk. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact This was a selected talk at the British Society for Research on Ageing conference in Oxford.
Year(s) Of Engagement Activity 2018
 
Description British Society for Research on Ageing Conference - Exeter, UK. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact This was an invited talk to the yearly meeting of the British Society for Research on Ageing. The talk was given in front of some 60 UK researchers into ageing.
Year(s) Of Engagement Activity 2017
 
Description Food for Healthy Ageing Conference - Amsterdam, Netherlands. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact This was an invited talk at the Food for Healthy Ageing Conference that took place in Amsterdam, Netherlands. The audience was composed of a range of professions, including scientists, science-related professions and nutritionists. I spoke on the interactions between ageing and nutrition.
Year(s) Of Engagement Activity 2017
 
Description Guest on "Slightly evolved" podcast 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact Featured as guest/interviewee on podcast featuring informal discussion of own work.
Year(s) Of Engagement Activity 2017
 
Description Institute of Cancer Research - Departmental Seminar 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Professional Practitioners
Results and Impact This was an invited scientific talk at the Institute of Cancer Research.
Year(s) Of Engagement Activity 2018
 
Description Invited talk on Foxo biology - Babraham Institute 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Postgraduate students
Results and Impact A research team at Babraham institute attended my talk on foxo biology in Drosophila. This instigated a debate/discussion of how this could be relevant to their area of research.
Year(s) Of Engagement Activity 2016
 
Description Invited talk on insulin signalling - Babraham Institute 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Postgraduate students
Results and Impact I was invited to give a talk to the Babraham Institue about insulin signalling and ageing in Drosophila. The talk was well received and had an impact on the opinios of the audience.
Year(s) Of Engagement Activity 2016
 
Description Pint of Science Festival - "Our Body" session 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Presenting and discussing with the public our research on the reprogramming of gene expression and lifespan by sugar and FoxO transcription factors. Part of a wider series of engagement activities in the Pint of Science festival.
Year(s) Of Engagement Activity 2017
URL http://www.pintofscience.com
 
Description Poster presentation of Dobson et al Cell Reports, Champalimaud Centre for the Unknown 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Poster presentation at meeting discussion developmental physiology and disease.
Year(s) Of Engagement Activity 2017
 
Description Poster presentation of Dobson et al Cell Reports, European Drosophila Research Conference 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Poster presentation at international meeting of Drosophila researchers.
Year(s) Of Engagement Activity 2017
 
Description Poster presentation on sugar, FoxO and ageing - BSRA meeting, Durham. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Poster presentation at the British Society for Research on Ageing, Durham, summer 2016. The poster presented work on reprogramming of gene expression and lifespan by sugar and FoxO.
Year(s) Of Engagement Activity 2016
 
Description Poster presentation on sugar, FoxO and ageing - UCL Biosciences Research Day, London. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Postgraduate students
Results and Impact The poster presented work on reprogramming of gene expression and lifespan by sugar and FoxO.
Year(s) Of Engagement Activity 2016
 
Description Poster presentation, Jena Ageing Meeting, Germany. 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Poster presentation describing the principal findings of the project.
Year(s) Of Engagement Activity 2018
 
Description Postgraduate teaching on Ageing - PhD seminars - Gulbenkian Science Institute, Portugal. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact 30 postgraduate students attended a half-day seminar/workshop that I conducted on ageing. The students were from the Gulbenkian Science Institute PhD programme. The event took place in their Institute in Portugal. This was an invited seminar/workshop.
Year(s) Of Engagement Activity 2017
 
Description Presentation of Dobson et al Cell Reports, Bateson Centre Lifecourse Biology Symposium, Sheffield. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Oral presentation at symposium marking opening of The Bateson Centre, Sheffield.
Year(s) Of Engagement Activity 2017
 
Description Presentation of Dobson et al Cell Reports, Free University Berlin 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Presentation to scientific audience
Year(s) Of Engagement Activity 2017
 
Description Presentation of Dobson et al Cell Reports, Imperial College London 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Postgraduate students
Results and Impact Scientific talk to postgraduate students
Year(s) Of Engagement Activity 2017
 
Description Presentation of Dobson et al Cell Reports, Technical University Dresden 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Talk to scientific audience
Year(s) Of Engagement Activity 2017
 
Description Presentation on FoxO biology and diet - Monash University. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact This was an invited talk to present our recent findings (Dobson et al. Cell Reports, 2017) to our collaborator and the students/staff at Monash University. The talk initiated a discussion that formed the ideas that will result in further collaborations.
Year(s) Of Engagement Activity 2017
 
Description Presentation on FoxO biology and diet - Sydney University. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact I was inited to give a talk at Sydney University about FoxO biology and diet (to the group led by Prof. Stephen Simpson). This was an opportunity for me to interact with the researchers and gain knowledge about nutritional biology in Drosophila and other organisms.
Year(s) Of Engagement Activity 2017
 
Description Press release for Filer et al Nature 2017 publication 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Media (as a channel to the public)
Results and Impact We composed a press release for the Filer et all Nature 2017 publication that we distributed by UCL. The press release was picked up by several online science pubications.
Year(s) Of Engagement Activity 2017
URL http://www.ucl.ac.uk/news/news-articles/1117/301117-worms-and-flies-live-longer
 
Description Press release for publication 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Media (as a channel to the public)
Results and Impact Press release for paper (PMID 29675265) from UCL press office.
Year(s) Of Engagement Activity 2018
 
Description Talk on dietary restriction, longevity and transcriptional control - Rank Prize Funds Symposium on Dietary Restriction and Ageing, Windermere, UK. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Talk at a special interest group retreat, presenting and discussing new research on dietary restriction and ageing. The meeting was attended by senior academics from around the world. The talk was well received and provided a platform for future collaborations. A publication detailing the major outputs of the meeting is planned.
Year(s) Of Engagement Activity 2016
 
Description UCL - Japan Youth Challenge - invited talk 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Schools
Results and Impact This was an event organised by and for school students from Japan and the UK. I was invited to talk.
Year(s) Of Engagement Activity 2018
URL https://www.ucl-japan-youth-challenge.com/
 
Description UCL Pharma Society - invited talk 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Undergraduate students
Results and Impact I was invited by student members of the UCL Pharma Society to talk about drugs that act against ageing.
Year(s) Of Engagement Activity 2018
 
Description University of Kent - Departmental seminar - invited talk 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Professional Practitioners
Results and Impact I gave an invited talk at the University of Kent to staff and students. The talks are organised by the PhD students.
Year(s) Of Engagement Activity 2018
 
Description Wellcome Trust Conference - Healthy Ageing 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact This was a formal scientific presentation for the international community of researcher interested in various aspects of the biology of ageing.
Year(s) Of Engagement Activity 2018
 
Description Wellcome Trust conference on Healthy Ageing - Pol III and lifespan - Selected talk. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact This was an invited scientific talk at a conference attended by an international audience of experts in the field.
Year(s) Of Engagement Activity 2018