"Behavioural assays for structural plasticity and repair in the central nervous system of Drosophila"

Lead Research Organisation: University of Birmingham
Department Name: Sch of Biosciences

Abstract

Germany

Publications

10 25 50
 
Description Learned methods new to my team. Most particularly, this involved techniques to investigate behaviour in Drosophila: the Buridan arena and optomotor response for the visual system, and learning and memory. The results obtained with this grant form part of a larger project.

This work is part of a larger body of work, which has just recently been published in eLife:

Guiyi Li, Manuel G Forero, Jill S Wentzell, Ilgim Durmus, Reinhard Wolf, Niki C Anthoney, Mieczyslaw Parker, Ruiying Jiang, Jacob Hasenauer, Nicholas James Strausfeld, Martin Heisenberg, Alicia Hidalgo (2020) "A Toll receptor map underlies structural brain plasticity" eLife DOI: 10.7554/eLife.52743
Exploitation Route it has brought new techniques to my lab.

Published in Li et al 2020 eLife DOI: 10.7554/eLife.52743, see link above
Sectors Healthcare,Pharmaceuticals and Medical Biotechnology

URL https://elifesciences.org/articles/52743
 
Description The findings from this grant were used in a recent publication: Guiyi Li, Manuel G Forero, Jill S Wentzell, Ilgim Durmus, Reinhard Wolf, Niki C Anthoney, Mieczyslaw Parker, Ruiying Jiang, Jacob Hasenauer, Nicholas James Strausfeld, Martin Heisenberg, Alicia Hidalgo (2020) "A Toll-receptor map underlies structural brain plasticity" eLife DOI: 10.7554/eLife.52743 we frequently carry out outreach activities in which all team members are involved, and we explain the use of behaviroual assays in flies
First Year Of Impact 2020
Sector Education,Healthcare,Pharmaceuticals and Medical Biotechnology
 
Description BBSRC ISIS
Amount £3,071 (GBP)
Funding ID BB/P004997/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 06/2016 
End 08/2016
 
Title New DeadEasy plugins to investigate brain plasticity 
Description We develop several new DeadEasy plugins, to count automatically cell number in the adult brain of the fruit-fly Drosophila: (1) DeadEasy Optic Lobe; (2) DeadEasy Central Brain; (3) DeadEasy Kenyon Cells. These were used in the publication Li et al 2020 eLife. 
Type Of Material Technology assay or reagent 
Year Produced 2020 
Provided To Others? Yes  
Impact This has only just been published, so impacts have not materialised yet. 
URL https://elifesciences.org/articles/52743
 
Title Novel DeadEasy plugins to investigate brain plasticity in Drosophila 
Description We have generated novel DeadEasy plugin to investigate brain plasticity in Drosophila. They enable us to automatically count cells throughout the brain: (1) DeadEasy Optic lobes for smaller cells; (2) DeadEasy Central Brain; (3) DeadEasy Kenyon Cells, for tightly packed cells. These plugins have been used in our publication Li et al 2020 eLife 
Type Of Material Computer model/algorithm 
Year Produced 2020 
Provided To Others? Yes  
Impact This has only just been published so impact still unknown 
URL https://elifesciences.org/articles/52743
 
Description "Automatic quantification of cell number in the brain of Drosophila" 
Organisation University of Ibagué
Country Colombia 
Sector Academic/University 
PI Contribution Contribution by my team: biological research data.
Collaborator Contribution Contribution by collaborating team: software produced to analyse our data.
Impact Recent publication in eLife: Guiyi Li, Manuel G Forero, Jill S Wentzell, Ilgim Durmus, Reinhard Wolf, Niki C Anthoney, Mieczyslaw Parker, Ruiying Jiang, Jacob Hasenauer, Nicholas James Strausfeld, Martin Heisenberg, Alicia Hidalgo (2020) "A Toll-receptor map underlies structural brain plasticity" eLife DOI: 10.7554/eLife.52743
Start Year 2018
 
Description Behavioural assays for structural plasticity and repair in the central nervous system of Drosophila 
Organisation University of Wurzburg
Country Germany 
Sector Academic/University 
PI Contribution The aim of this collaboration was for my research team to learn from our collaborators about behaviour assays in fruit-flies. Once we have data ready for publication, we will offer them authorship in a joint paper.
Collaborator Contribution We collaborated with Prof Martin Heisenberg and Dr Reinhard Wolf. One member of my team visited their lab to learn behaviour assays for fruit-flies. This helps our research by enriching the experimental approaches we can use to address our questions.
Impact Behavioural analysis in flies. Outcomes not ready yet, work in progress.
Start Year 2016
 
Description Collaboration with Professor Martin Heisenberg and Reinhard Wolf, University of Würzburg, Germany 
Organisation University of Wurzburg
Country Germany 
Sector Academic/University 
PI Contribution Guiyi Li from my lab went to the lab of Professor Martin Heisenberg to learn to carry out behaviour experiments including: the Buridan arena essay, the optomotor response and learning and memory. She brought these techniques back to the lab, as well as some kit made in the Würzburg workshop.
Collaborator Contribution Teaching staff from my lab to cary out some techniques, and providing us with kit to carry out those experiments. It is difficult to estimate the commercial value of the kit (hence the reason for not declaring it as an 'in kind' contribution, as they were designed and made by our collaborators' workshop, but they're extremely useful for our research.
Impact We have just recently submitted an article for publication that resulted from this collaboration: Li et al (submitted) A Toll-receptor map underlies structural brain plasticity.
Start Year 2016
 
Title DeadEasy Central Brain 
Description DeadEasy Central Brain counts automatically in vivo all the nuclei in the Drosophila adult central brain, labelled with histone-YFP. Will count other nuclei of similar size, stained with nuclear markers. 
Type Of Technology Software 
Year Produced 2020 
Impact recently published in Li et al 2020 eLife 
URL https://elifesciences.org/articles/52743
 
Title DeadEasy Kenyon Cells 
Description DeadEasy Kenyon Cells counts automatically in vivo the tightly packed cells of the mushroom bodies of Drosophila, labelled with histone-YFP. Will count other similar nuclei. 
Type Of Technology Software 
Year Produced 2020 
Impact recently published, impacts not materialised yet 
URL https://elifesciences.org/articles/52743
 
Title DeadEasy Optic Lobe 
Description DeadEasy Optic Lobes counts automatically the many and tiny cell nuclei labelled with Histone-YFP of the Drosophila adult optic lobes, in 3D, in vivo. It will count other cells of similar size, visible with fluorescent nuclear markers. 
Type Of Technology Software 
Year Produced 2020 
Impact Published in Li et al 2020 eLife 
URL https://elifesciences.org/articles/52743