China Partnering Award: Emerging Approaches to Intracellular Signaling

Lead Research Organisation: University of Leeds
Department Name: School of Biomedical Sciences


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Description This is still early stage of the project but we made a good progress in identifying interactions between the ANO1-like protein TMC7 and TRPV1 in mammalian sensory neurons.

We also investigated molecular composition of the Kv4 potassium channel complex. This work has been published in the FASEB Journal
Exploitation Route At this time the findings are interesting but still preliminary. The visiting scheme worked excellently before the lockdown and we hope to be able to resume.
Sectors Education,Pharmaceuticals and Medical Biotechnology

Description Divide and rule: localised Ca2+ signalling in sensory neurons
Amount £567,770 (GBP)
Funding ID BB/V010344/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 07/2021 
End 06/2024
Title A correlative super-resolution protocol to visualise structural underpinnings of fast second-messenger signalling in primary cell types 
Description A correlative imaging protocol which allows the ubiquitous intracellular second messenger, calcium (Ca2+), to be directly visualised against nanoscale patterns of the Ca2+ channels in primary cells. This is achieved by combining total internal reflection fluorescence (TIRF) imaging of the elementary Ca2+ signals, with the subsequent DNA-PAINT super-resolution imaging of the Ca2+ channels. 
Type Of Material Technology assay or reagent 
Year Produced 2020 
Provided To Others? Yes  
Impact Most super-resolution microscopy methods require fixed cells/tissue. Here we developed a correlative approach in which live signaling events recorded from the living cells can be matched to the nanoscale structural information obtained with super-resolution imaging. This will further the scope of super-resolution imaging. 
Title All-optical monitoring of chloride channel activity in living cells 
Description We developed a triple-wavelength fluorescent imaging approach to simultaneously perform halide-sensitive EYFP quenching (to measure Cl- channel activity) and ratiometric fura-2 Ca2+ imaging. The method can be used as a higher-throughput alternative to patch-clamp for studying Ca2+-activated Cl- channels. 
Type Of Material Technology assay or reagent 
Year Produced 2019 
Provided To Others? No  
Impact The publication is currently under revision.