Mass Spectrometry Based Lipidomics and Metabolomics to Drive Bioscience Discovery
Lead Research Organisation:
Swansea University
Department Name: Institute of Life Science Medical School
Abstract
Lipids are key components of biological system ranging from plants to mammals. Lipid molecules are critically involved in membrane structures of cells and also provide an energy store and act as signalling molecules within and between cells. Hence, understanding lipid metabolism is important for all areas of the BBSRC remit.
Mass spectrometry (MS) represents the primary technology in lipidomics and when combined with liquid chromatography (LC) is suitable for the unbiased identification and quantification of lipids in complex mixtures. Extra dimensions of separation are provided by high resolution MS, where isobaric ions (ions with the same nominal mass but different exact mass) are resolved, and by tandem mass spectrometry (MS/MS) or multi-stage fragmentation (MSn), where characteristic ions are separated from background. A more recent addition to MS-tool box is MS-imaging (MSI) adding further to the available bioimaging technologies.
The UK has a burgeoning lipidomic community but lacks a MSI resource. The current proposal is for a high resolution MS instrument with MS/MS and MSn capabilities combined with interchangeable interfaces for high spatial-resolution atmospheric pressure (AP)-MALDI and electrospray ionisation (ESI). The AP-MALDI source offers high spatial-resolution (5 - 10 microm), at the single cell level, while the ESI interface is compatible with other imaging modalities e.g. Liquid Extraction for Surface Analysis (LESA). The instrument will be a resource for BBRSC-remit research and for the UK lipidomic community both academic and industrial. The new instrument will underpin scientific research in several key strategic priority areas for BBSRC, including (1) Healthy ageing across the life course; (2) Sustainably enhancing agricultural production; and (3) The replacement, refinement and reduction (3Rs) in research using animals.
Mass spectrometry (MS) represents the primary technology in lipidomics and when combined with liquid chromatography (LC) is suitable for the unbiased identification and quantification of lipids in complex mixtures. Extra dimensions of separation are provided by high resolution MS, where isobaric ions (ions with the same nominal mass but different exact mass) are resolved, and by tandem mass spectrometry (MS/MS) or multi-stage fragmentation (MSn), where characteristic ions are separated from background. A more recent addition to MS-tool box is MS-imaging (MSI) adding further to the available bioimaging technologies.
The UK has a burgeoning lipidomic community but lacks a MSI resource. The current proposal is for a high resolution MS instrument with MS/MS and MSn capabilities combined with interchangeable interfaces for high spatial-resolution atmospheric pressure (AP)-MALDI and electrospray ionisation (ESI). The AP-MALDI source offers high spatial-resolution (5 - 10 microm), at the single cell level, while the ESI interface is compatible with other imaging modalities e.g. Liquid Extraction for Surface Analysis (LESA). The instrument will be a resource for BBRSC-remit research and for the UK lipidomic community both academic and industrial. The new instrument will underpin scientific research in several key strategic priority areas for BBSRC, including (1) Healthy ageing across the life course; (2) Sustainably enhancing agricultural production; and (3) The replacement, refinement and reduction (3Rs) in research using animals.
Technical Summary
The main aim of this proposal is to establish a mass spectrometry imaging (MSI) resource for the BBSRC lipidomics community in the UK. This will be achieved through the siting in Swansea University of an MSI instrument capable of high spatial-resolution (5 - 10 microm), high mass-resolution (>200,000 (FWHM) at m/z 400) and capacity to perform tandem-MS (MS/MS) and multistage-fragmentation (MSn). The instrument will be equipped with a AP-MALDI source with 10 kHz Nd:YAG laser at 355 nm and spot size of <10 microm. An advantage of the AP-MALDI source is that it can be rapidly interchanged with an electrospray ionisation (ESI) source, minimising down-time between different applications. The ESI source can be utilised with other imaging modalities, e.g. Liquid Extraction for Surface Analysis (LESA). The mass spectrometer will have a resolution of >200,000 (FWHM) at m/z 400, allowing separation of lipid signals from isobaric (ions with the same nominal mass but different exact mass) chemical noise. This will be important for the imaging of low-abundance lipids. The instrument will be able to perform both MS/MS and MSn with high mass-resolution and high mass-accuracy measurement of fragment-ions. MS/MS will add an extra dimension to MSI allowing the exploitation of multiple-reaction monitoring methods commonly used for LC-MS applications to enhance signal to noise ratio. In addition, MSn is a particularly important fragmentation method for the structural identification of unknown lipids.
Planned Impact
This application is to install a high spatial-resolution mass spectrometry imaging (MSI) instrument at Swansea University. The instrument will be a resource for BBRSC-remit research and the UK lipidomic community. As illustrated in the application work-packages, the equipment will drive bioscience discovery in a breadth of research areas ranging from food and agriculture to animal and human health and consequently have significant social and economic impact.
Impact on food and nutrition: Global consumption of edible oil has increased steadily at an annual rate of about 5% for the past 50 years. Innovation in oilseed technology is required to create a hybrid vegetable oil replacement for fish oil and for production of high value oils. High spatial-resolution MSI will elucidate the compartmentation of oil assembly in developing seeds, which is essential for the redesign of lipid biosynthesis in seeds in order to improve yield.
Impact on agriculture: A major limitation to plant growth is restricted access to nutrients in the soil. To improve nutrient acquisition, most land plants enter a beneficial symbiosis with arbuscular mycorrhizal (AM) fungi. Incomplete understanding of the mechanism underlying this symbiosis makes it difficult to exploit for plant crop improvement. A recent discovery made at Rothamsted Research that plants supply lipids to arbuscular mycorrhiza was a paradigm shift. MSI will now provide a complete spatial understanding of lipid exchange and allow us to learn how to facilitate this process for agricultural benefit.
At Swansea University, a patented (WO2016198852A1) technology "symbiont-mediated RNA interference" was developed to eliminate insect pests. Lipidomics will be used to identify the RNAi target in insects to prevent transmission of a plant pathogenic virus. This virus has a huge worldwide impact on agricultural productivity. Current control strategies for agricultural insect pests are heavily reliant on chemical pesticides. However, there is no safe targeted chemical pesticide. This research aims to replace harmful non-targeted chemical pesticides with a smart, environmentally-friendly alternatives and so improve worldwide agricultural productivity. In addition, the technology could be translated to limit transmission of viruses by insects that cause human and animal disease.
Impact on animal and human health: Lipid metabolism underpins normal physiology and homeostatic control during early development and across the lifespan. One particular example is cholesterol metabolism. Cholesterol is implicated in various neurological disorders and much more research into the involvement of cholesterol metabolism in healthy brain function is still required. We will use the proposed instrument with Swansea University patented (US9851368B2) technology to generate the first 3D-attlas of the sterol content of brain, which will fill significant gaps in our understanding of brain biology across the life span and also generate considerable interest from academics, clinicians, the pharmaceutical industry and the public in general.
Impact with 3Rs: Through better characterisation of the metabolic consequences of toxin/carcinogen exposure to human 3D tissue models, we will validate these models as an alternative approach to animal testing. This will impact the safety assessment in chemical and pharmaceutical industries and offer the possibility of replacing animal use in future.
Impact on drug discovery: Improved methods and technologies to image drugs and their metabolites in tissue will impact the drug discovery process
Impact on training for the lipidomics community: The availability of a MSI lipidomic community resource, also available for young researchers to access, will greatly improve training options available in the UK.
Impact on food and nutrition: Global consumption of edible oil has increased steadily at an annual rate of about 5% for the past 50 years. Innovation in oilseed technology is required to create a hybrid vegetable oil replacement for fish oil and for production of high value oils. High spatial-resolution MSI will elucidate the compartmentation of oil assembly in developing seeds, which is essential for the redesign of lipid biosynthesis in seeds in order to improve yield.
Impact on agriculture: A major limitation to plant growth is restricted access to nutrients in the soil. To improve nutrient acquisition, most land plants enter a beneficial symbiosis with arbuscular mycorrhizal (AM) fungi. Incomplete understanding of the mechanism underlying this symbiosis makes it difficult to exploit for plant crop improvement. A recent discovery made at Rothamsted Research that plants supply lipids to arbuscular mycorrhiza was a paradigm shift. MSI will now provide a complete spatial understanding of lipid exchange and allow us to learn how to facilitate this process for agricultural benefit.
At Swansea University, a patented (WO2016198852A1) technology "symbiont-mediated RNA interference" was developed to eliminate insect pests. Lipidomics will be used to identify the RNAi target in insects to prevent transmission of a plant pathogenic virus. This virus has a huge worldwide impact on agricultural productivity. Current control strategies for agricultural insect pests are heavily reliant on chemical pesticides. However, there is no safe targeted chemical pesticide. This research aims to replace harmful non-targeted chemical pesticides with a smart, environmentally-friendly alternatives and so improve worldwide agricultural productivity. In addition, the technology could be translated to limit transmission of viruses by insects that cause human and animal disease.
Impact on animal and human health: Lipid metabolism underpins normal physiology and homeostatic control during early development and across the lifespan. One particular example is cholesterol metabolism. Cholesterol is implicated in various neurological disorders and much more research into the involvement of cholesterol metabolism in healthy brain function is still required. We will use the proposed instrument with Swansea University patented (US9851368B2) technology to generate the first 3D-attlas of the sterol content of brain, which will fill significant gaps in our understanding of brain biology across the life span and also generate considerable interest from academics, clinicians, the pharmaceutical industry and the public in general.
Impact with 3Rs: Through better characterisation of the metabolic consequences of toxin/carcinogen exposure to human 3D tissue models, we will validate these models as an alternative approach to animal testing. This will impact the safety assessment in chemical and pharmaceutical industries and offer the possibility of replacing animal use in future.
Impact on drug discovery: Improved methods and technologies to image drugs and their metabolites in tissue will impact the drug discovery process
Impact on training for the lipidomics community: The availability of a MSI lipidomic community resource, also available for young researchers to access, will greatly improve training options available in the UK.
Organisations
- Swansea University (Lead Research Organisation)
- University Hospital Regensburg (Collaboration)
- Washington University in St Louis (Collaboration)
- University of Basel (Collaboration)
- Karolinska Institute (Collaboration)
- University of Oslo (Collaboration)
- University of Regensburg (Collaboration)
- Athens Medical Center (Collaboration)
- University of Southern Denmark (Collaboration)
- University of Clermont Auvergne (Collaboration)
- UNIVERSITY OF EDINBURGH (Collaboration)
- Woods Hole Oceanographic Institution (Collaboration)
- Cardiff University (Collaboration)
- Rothamsted Research (Collaboration)
- Biotechnology and Biological Sciences Research Council (BBSRC) (Collaboration)
- University of Illinois at Chicago (Collaboration)
- University of Franche-Comté (Collaboration)
- Novartis (Collaboration)
- Babraham Institute (Collaboration)
- Nebraska Medical Center (Collaboration)
- Université Catholique de Louvain (Collaboration)
- Luxembourg Institute of Science and Technology (Collaboration)
- UNIVERSITY OF OXFORD (Collaboration)
- University of Toulouse (Collaboration)
- Medical University of Graz (Collaboration)
- National University of Singapore (Collaboration)
- University of Pardubice (Collaboration)
- Minerva Foundation Institute for Medical Research (Collaboration)
- University of Texas Southwestern Medical Center (Collaboration)
Publications

Yutuc E
(2021)
Deep mining of oxysterols and cholestenoic acids in human plasma and cerebrospinal fluid: Quantification using isotope dilution mass spectrometry.
in Analytica chimica acta

Daniilidou M
(2023)
Alzheimer's disease biomarker profiling in a memory clinic cohort without common comorbidities.
in Brain communications

Wang Y
(2021)
Neuro-oxysterols and neuro-sterols as ligands to nuclear receptors, GPCRs, ligand-gated ion channels and other protein receptors.
in British journal of pharmacology

De La Fuente DC
(2024)
Impaired oxysterol-liver X receptor signaling underlies aberrant cortical neurogenesis in a stem cell model of neurodevelopmental disorder.
in Cell reports

Reed J
(2024)
The Regulation of Metabolic Homeostasis by Incretins and the Metabolic Hormones Produced by Pancreatic Islets
in Diabetes, Metabolic Syndrome and Obesity

Reed J
(2021)
A Review of Current Trends with Type 2 Diabetes Epidemiology, Aetiology, Pathogenesis, Treatments and Future Perspectives.
in Diabetes, metabolic syndrome and obesity : targets and therapy

Dickson AL
(2022)
Identification of unusual oxysterols biosynthesised in human pregnancy by charge-tagging and liquid chromatography - mass spectrometry.
in Frontiers in endocrinology

Hennegan J
(2024)
Inhibition of 7a,26-dihydroxycholesterol biosynthesis promotes midbrain dopaminergic neuron development.
in iScience

Dickson A
(2023)
HSD3B1 is an oxysterol 3ß-hydroxysteroid dehydrogenase in human placenta.
in Open biology

Kanamarlapudi V
(2022)
ADP-ribosylation factor 6 expression increase in oesophageal adenocarcinoma suggests a potential biomarker role for it
in PLOS ONE
Description | As part of this award we have developed further ultra-high sensitivity MALDI-mass spectrometry imaging (MSI) at high spatial resolution for analysis of sterols and phospholipids. This award has facilitated collaborations as part of the SWBio Doctoral Training Partnership, and increased training capabilities available to the DTP. The award has allowed the establishment of new collaborations with Babraham Institute, Cambridge University, Cardiff University, Rothamsted Research and Edinburgh University. |
Exploitation Route | The MALDI-MSI methods developed further and exploited in this grant can be utilised by other academics and commercial companies. |
Sectors | Agriculture Food and Drink Chemicals Environment Healthcare Manufacturing including Industrial Biotechology Pharmaceuticals and Medical Biotechnology |
Description | Using AP-MALDI supplied by KR Analytical Ltd we have reduced the pixel size to <0.01 mm for mass spectrometry imaging of cholesterol. We have incorporated multi-stage-fragmentation with AP-MALDI for mass spectrometry imaging of sterols. These developments will contribute to the economic competitiveness of the UK company supplying the instrumentation. Using the instrument funded by this award we have optimised a protocol to allow the measurement of almost all cholesterol metabolites in a single single assay. We are working with the pharmaceutical industry to exploit this in the drug discovery process and with the NHS to assist in the diagnosis of patients with sterol related disorders. |
First Year Of Impact | 2020 |
Sector | Healthcare,Pharmaceuticals and Medical Biotechnology |
Description | Department of Health workshop on non-genetic conditions |
Geographic Reach | National |
Policy Influence Type | Contribution to a national consultation/review |
Description | Introducing the Lipidomics Minimal Reporting Checklist |
Geographic Reach | Multiple continents/international |
Policy Influence Type | Contribution to new or improved professional practice |
URL | https://lipidomicssociety.org/interest_groups/lipidomics-standards-initiative-lsi/ |
Description | NHS Wales Rare Diseases Implementation Group |
Geographic Reach | National |
Policy Influence Type | Participation in a guidance/advisory committee |
Description | A 3D Neurosterol Atlas of Mouse Brain |
Amount | £450,711 (GBP) |
Funding ID | BB/T018542/1 |
Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
Sector | Public |
Country | United Kingdom |
Start | 12/2020 |
End | 12/2024 |
Description | Purchase of equipment: The SunCollect System for MALDI imaging sample preparation |
Amount | £24,500 (GBP) |
Funding ID | MA/KW/5554/19 |
Organisation | Government of Wales |
Sector | Public |
Country | United Kingdom |
Start | 02/2022 |
End | 03/2022 |
Description | Sterol Characterization and Biomarker Discovery in PD CSF and Plasma |
Amount | £258,940 (GBP) |
Funding ID | MJFF-021150 |
Organisation | Michael J Fox Foundation |
Sector | Charity/Non Profit |
Country | United States |
Start | 01/2022 |
End | 08/2023 |
Description | Understanding and manipulating how Trypanosoma cruzi infects its triatomine insect hosts |
Amount | £390,138 (GBP) |
Funding ID | BB/Y001125/1 |
Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
Sector | Public |
Country | United Kingdom |
Start | 12/2023 |
End | 11/2026 |
Title | Pregnancy Sterols |
Description | Mass spectrometry raw data from the study of pregnancy sterols described in DOI: 10.3389/fendo.2022.1031013 and doi: https://doi.org/10.1101/2022.04.01.486576 |
Type Of Material | Database/Collection of data |
Year Produced | 2022 |
Provided To Others? | Yes |
Impact | Catalyst for new collaborations. |
URL | https://osf.io/egncz/ |
Description | Bile Acid Ring Trial |
Organisation | National University of Singapore |
Country | Singapore |
Sector | Academic/University |
PI Contribution | We are one of a number of groups world wide providing bile acid measurement on a standard reference material. |
Collaborator Contribution | We are one of a number of groups world wide providing bile acid measurement on a standard reference material. Commercial partners are providing authentic standards free of charge. |
Impact | An expert protocol has been developed for bile acid analysis in human plasma. |
Start Year | 2020 |
Description | Bjorkhem |
Organisation | Karolinska Institute |
Country | Sweden |
Sector | Academic/University |
PI Contribution | We provide expertise in LC-MS analysis of sterols. |
Collaborator Contribution | Our partner provides transgenic mouse material, human plasma and CSF. |
Impact | doi: 10.1194/jlr.P048603 doi: 10.1172/JCI68506 doi: 10.1016/j.jsbmb.2016.03.018 doi: 10.1016/j.biochi.2018.07.016 doi: 10.1074/jbc.RA118.005639 doi: 10.1016/j.jsbmb.2019.03.025 DOI: 10.1126/sciadv.adj1354 DOI: 10.1093/braincomms/fcad228 doi: 10.1016/j.jsbmb.2019.105475 doi: 10.1016/j.jsbmb.2020.105794 |
Start Year | 2007 |
Description | Cardiff Brain |
Organisation | Cardiff University |
Country | United Kingdom |
Sector | Academic/University |
PI Contribution | We have analysed brain tissue from transgenic animals. |
Collaborator Contribution | Our partners have provided brain tissue from transgenic animals. |
Impact | This collaboration has resulted in multiple grant applications. |
Start Year | 2020 |
Description | Cardiff Neuroscience |
Organisation | Cardiff University |
Country | United Kingdom |
Sector | Academic/University |
PI Contribution | Analysis of cultured cells for oxysterols |
Collaborator Contribution | Provision of cellular material |
Impact | Manuscript in preparation. Publication DOI: 10.1016/j.isci.2023.108670 |
Start Year | 2014 |
Description | ENOR |
Organisation | Catholic University of Louvain |
Country | Belgium |
Sector | Academic/University |
PI Contribution | expertise, intellectual input, access to equipment |
Collaborator Contribution | Access to biological materials |
Impact | doi: 10.1016/j.jsbmb.2019.03.02 doi: 10.1016/j.biochi.2018.07.016 DOI: 10.1016/j.jsbmb.2024.106495 |
Start Year | 2010 |
Description | ENOR |
Organisation | Minerva Foundation Institute for Medical Research |
Country | Finland |
Sector | Private |
PI Contribution | expertise, intellectual input, access to equipment |
Collaborator Contribution | Access to biological materials |
Impact | doi: 10.1016/j.jsbmb.2019.03.02 doi: 10.1016/j.biochi.2018.07.016 DOI: 10.1016/j.jsbmb.2024.106495 |
Start Year | 2010 |
Description | ENOR |
Organisation | Novartis |
Country | Global |
Sector | Private |
PI Contribution | expertise, intellectual input, access to equipment |
Collaborator Contribution | Access to biological materials |
Impact | doi: 10.1016/j.jsbmb.2019.03.02 doi: 10.1016/j.biochi.2018.07.016 DOI: 10.1016/j.jsbmb.2024.106495 |
Start Year | 2010 |
Description | ENOR |
Organisation | University Hospital Regensburg |
Country | Germany |
Sector | Hospitals |
PI Contribution | expertise, intellectual input, access to equipment |
Collaborator Contribution | Access to biological materials |
Impact | doi: 10.1016/j.jsbmb.2019.03.02 doi: 10.1016/j.biochi.2018.07.016 DOI: 10.1016/j.jsbmb.2024.106495 |
Start Year | 2010 |
Description | ENOR |
Organisation | University of Basel |
Country | Switzerland |
Sector | Academic/University |
PI Contribution | expertise, intellectual input, access to equipment |
Collaborator Contribution | Access to biological materials |
Impact | doi: 10.1016/j.jsbmb.2019.03.02 doi: 10.1016/j.biochi.2018.07.016 DOI: 10.1016/j.jsbmb.2024.106495 |
Start Year | 2010 |
Description | ENOR |
Organisation | University of Clermont Auvergne |
Country | France |
Sector | Academic/University |
PI Contribution | expertise, intellectual input, access to equipment |
Collaborator Contribution | Access to biological materials |
Impact | doi: 10.1016/j.jsbmb.2019.03.02 doi: 10.1016/j.biochi.2018.07.016 DOI: 10.1016/j.jsbmb.2024.106495 |
Start Year | 2010 |
Description | ENOR |
Organisation | University of Edinburgh |
Department | Queen's Medical Research Institute Edinburgh |
Country | United Kingdom |
Sector | Academic/University |
PI Contribution | expertise, intellectual input, access to equipment |
Collaborator Contribution | Access to biological materials |
Impact | doi: 10.1016/j.jsbmb.2019.03.02 doi: 10.1016/j.biochi.2018.07.016 DOI: 10.1016/j.jsbmb.2024.106495 |
Start Year | 2010 |
Description | ENOR |
Organisation | University of Franche-Comté |
Country | France |
Sector | Academic/University |
PI Contribution | expertise, intellectual input, access to equipment |
Collaborator Contribution | Access to biological materials |
Impact | doi: 10.1016/j.jsbmb.2019.03.02 doi: 10.1016/j.biochi.2018.07.016 DOI: 10.1016/j.jsbmb.2024.106495 |
Start Year | 2010 |
Description | ENOR |
Organisation | University of Oslo |
Country | Norway |
Sector | Academic/University |
PI Contribution | expertise, intellectual input, access to equipment |
Collaborator Contribution | Access to biological materials |
Impact | doi: 10.1016/j.jsbmb.2019.03.02 doi: 10.1016/j.biochi.2018.07.016 DOI: 10.1016/j.jsbmb.2024.106495 |
Start Year | 2010 |
Description | ENOR |
Organisation | University of Toulouse |
Country | France |
Sector | Academic/University |
PI Contribution | expertise, intellectual input, access to equipment |
Collaborator Contribution | Access to biological materials |
Impact | doi: 10.1016/j.jsbmb.2019.03.02 doi: 10.1016/j.biochi.2018.07.016 DOI: 10.1016/j.jsbmb.2024.106495 |
Start Year | 2010 |
Description | Edinburgh Imaging |
Organisation | University of Edinburgh |
Country | United Kingdom |
Sector | Academic/University |
PI Contribution | We have provided expertise in enzyme assisted derivatisation for sterol analysis and in liquid extraction for surface analysis (LESA)-mass spectrometry imaging (MSI). This resulted in a successful grant application and on-going collaboration. We are imaging mouse brain in collaboration with Edinburgh. We are imaging sterols, they are imaging steroids. |
Collaborator Contribution | Our partners have provided expertise in on-tissue derivatisation and access to mouse tissue. They are contributing to the imaging project by imaging steroids to complement our sterol imaging. |
Impact | The collaboration has resulted in the successful joint grant application "A 3D Neurosterol Atlas of Mouse Brain" BB/T018542/1. Conference presentations: Constructing a Three-Dimensional Sterol Atlas of Mouse Brain: The First Steps. Eylan Yutuc. BMSS SIG Imaging Symposium, Sheffield, 2022. Constructing a Three-Dimensional Neurosterol Atlas of Mouse Brain. Eylan Yutuc. 42nd BMSS ANNUAL MEETING, Royal Northern College of Music, 13th-15th September 2022. Integration of Multimodal Mass Spec Imaging of Sterols with the Allen Mouse Brain Reference Atlas. Eylan Yutuc, Heath Patterson, Thao Tran, Shazia Khan, Maria Mantas, Nico Verbeeck, Alice Ly, Marc Claesen, Joyce Yau, Ruth Andrew, William J Griffiths, Yuqin Wang. 43rd BMSS ANNUAL MEETING, Royal Northern College of Music, 12th-14th September 2022. A 3D neurosteroids atlas of mouse brian using mass spectrometry imaging. Shazia Khan, Eylan Yutuc, Joyce L.W. Yau, Yuqin Wang , William J Griffiths & Ruth Andrew. Society for Endocrinology BES 2023, Glasgow, UK, 13 Nov 2023 - 15 Nov 2023. |
Start Year | 2019 |
Description | Lipidomics Standards Initiative |
Organisation | Babraham Institute |
Country | United Kingdom |
Sector | Academic/University |
PI Contribution | Intellectual input to the Lipidomics Standards Initiative. |
Collaborator Contribution | Intellectual input. |
Impact | doi.org/10.1038/s42255-019-0094-z |
Start Year | 2018 |
Description | Lipidomics Standards Initiative |
Organisation | Cardiff University |
Department | School of Medicine |
Country | United Kingdom |
Sector | Academic/University |
PI Contribution | Intellectual input to the Lipidomics Standards Initiative. |
Collaborator Contribution | Intellectual input. |
Impact | doi.org/10.1038/s42255-019-0094-z |
Start Year | 2018 |
Description | Lipidomics Standards Initiative |
Organisation | Medical University of Graz |
Country | Austria |
Sector | Academic/University |
PI Contribution | Intellectual input to the Lipidomics Standards Initiative. |
Collaborator Contribution | Intellectual input. |
Impact | doi.org/10.1038/s42255-019-0094-z |
Start Year | 2018 |
Description | Lipidomics Standards Initiative |
Organisation | University of Pardubice |
Country | Czech Republic |
Sector | Academic/University |
PI Contribution | Intellectual input to the Lipidomics Standards Initiative. |
Collaborator Contribution | Intellectual input. |
Impact | doi.org/10.1038/s42255-019-0094-z |
Start Year | 2018 |
Description | Lipidomics Standards Initiative |
Organisation | University of Regensburg |
Country | Germany |
Sector | Academic/University |
PI Contribution | Intellectual input to the Lipidomics Standards Initiative. |
Collaborator Contribution | Intellectual input. |
Impact | doi.org/10.1038/s42255-019-0094-z |
Start Year | 2018 |
Description | Lipidomics Standards Initiative |
Organisation | University of Southern Denmark |
Country | Denmark |
Sector | Academic/University |
PI Contribution | Intellectual input to the Lipidomics Standards Initiative. |
Collaborator Contribution | Intellectual input. |
Impact | doi.org/10.1038/s42255-019-0094-z |
Start Year | 2018 |
Description | Lipidomics Standards Initiative |
Organisation | University of Texas Southwestern Medical Center |
Country | United States |
Sector | Academic/University |
PI Contribution | Intellectual input to the Lipidomics Standards Initiative. |
Collaborator Contribution | Intellectual input. |
Impact | doi.org/10.1038/s42255-019-0094-z |
Start Year | 2018 |
Description | Luxembourg Institute of Science and Technology, |
Organisation | Luxembourg Institute of Science and Technology |
Department | GP Lippmann Public Research Institute (CRP) |
Country | Luxembourg |
Sector | Academic/University |
PI Contribution | We contributed expertise in cholesterol biochemistry and analysis. |
Collaborator Contribution | Our partner provided access to data, equipment and facilities. |
Impact | https://doi.org/10.1101/2020.11.06.369447 |
Start Year | 2019 |
Description | Oxford Centre for Diabetes, Endocrinology & Metabolism |
Organisation | University of Oxford |
Department | Oxford Hub |
Country | United Kingdom |
Sector | Academic/University |
PI Contribution | Expertise in the analysis of oxysterols |
Collaborator Contribution | Provision of valuable biological material |
Impact | Joint grant application made. |
Start Year | 2023 |
Description | Rothamsted Research |
Organisation | Rothamsted Research |
Country | United Kingdom |
Sector | Academic/University |
PI Contribution | We are providing expertise in mass spectrometry imaging. |
Collaborator Contribution | Our partners are providing expertise in plant science and plant material. |
Impact | Together we have a PhD student funded by the SWBio DTP. |
Start Year | 2019 |
Description | SPG5 at Athens Medical Center |
Organisation | Athens Medical Center |
Country | Greece |
Sector | Hospitals |
PI Contribution | Analysis of plasma and urine samples from SPG5 patients under a new treatment regime. |
Collaborator Contribution | Disease diagnosis. Treatment of patients. |
Impact | doi: 10.1016/j.biochi.2018.06.020; DOI: 10.1016/j.isci.2023.108670 Collaboration between Medicine and Bioanalysis. |
Start Year | 2015 |
Description | SWBIO |
Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
Department | South West Biosciences Doctoral Training Partnership |
Country | United Kingdom |
Sector | Academic/University |
PI Contribution | We have provided expertise and access to equipment and facilities. |
Collaborator Contribution | Our collaborators have provided access to expertise and materials. |
Impact | This is a multidisciplinary Doctoral Training Partnership. the current iteration of the Partnership is in its first accademic year. |
Start Year | 2020 |
Description | University of Illinois at Chicago, |
Organisation | University of Illinois at Chicago |
Country | United States |
Sector | Academic/University |
PI Contribution | We have provided expertise in cholesterol analysis in brain tissue. |
Collaborator Contribution | Our partners have provided transgenic animal material. |
Impact | https://doi.org/10.1101/2020.11.06.369447 |
Start Year | 2019 |
Description | University of Nebraska Medical Center |
Organisation | Nebraska Medical Center |
Country | United States |
Sector | Hospitals |
PI Contribution | We provided expertise for sterol analysis of brain tissue. |
Collaborator Contribution | Our partners provided transgenic animal materials and expertise on brain development. |
Impact | https://doi.org/10.1101/2020.11.06.369447 |
Start Year | 2019 |
Description | WOODS HOLE OCEANOGRAPHIC INSTITUTION |
Organisation | Woods Hole Oceanographic Institution |
Country | United States |
Sector | Charity/Non Profit |
PI Contribution | We have used our mass spectrometry and sterolomic technology to analyse biological samples. |
Collaborator Contribution | Woods Hole have provided biological samples. |
Impact | Grant application submitted to NIH |
Start Year | 2021 |
Description | Washington University in St. Louis - TB |
Organisation | Washington University in St Louis |
Country | United States |
Sector | Academic/University |
PI Contribution | We are analyzing materials for oxysterols. |
Collaborator Contribution | Washington University are providing material for oxysterol analysis |
Impact | Successful grant application NIH. Award number 1R01AI178685-01A1. Presentation "Cholesterol 25-hydroxylase promotes survival and modulates immune cell recruitment to the lung during Mycobacterium tuberculosis infection" at the 2024 American Thoracic Society annual meeting. |
Start Year | 2021 |
Description | Wellcome Centre for Human Genetics |
Organisation | University of Oxford |
Department | Oxford Hub |
Country | United Kingdom |
Sector | Academic/University |
PI Contribution | We are performing sterol analysis to clarify the biochemistry behind rare mutations in human and in a mouse model. |
Collaborator Contribution | Oxford are providing biological material. |
Impact | Grant application. |
Start Year | 2022 |
Description | 100 mins with ILS |
Form Of Engagement Activity | A broadcast e.g. TV/radio/film/podcast (other than news/press) |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Postgraduate students |
Results and Impact | Seminar to lipid scientists organised by the International lipidomic society |
Year(s) Of Engagement Activity | 2021 |
URL | https://lipidomicssociety.org/2021/05/17/100-minutes-with-ils-podcast/ |
Description | Cardiff Huntington's Disease Centre Public Engagement Event |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Patients, carers and/or patient groups |
Results and Impact | Presentation to the Cardiff Huntington's Disease Centre Public Engagement Event. Great interest from the patients and carers about the work we are doing to support future clinical trials. |
Year(s) Of Engagement Activity | 2023 |
Description | EpiLipidNET Work Group 1 |
Form Of Engagement Activity | A broadcast e.g. TV/radio/film/podcast (other than news/press) |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Postgraduate students |
Results and Impact | Talk via Zoom to postgraduate students and professionals interested in lipidomics |
Year(s) Of Engagement Activity | 2022 |
URL | https://www.cost.eu/actions/CA19105/#tabs+Name:Description |
Description | ILS Clinical Lipidomics |
Form Of Engagement Activity | A broadcast e.g. TV/radio/film/podcast (other than news/press) |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | On-line talk on Clinical Lipidomics. |
Year(s) Of Engagement Activity | 2022 |
URL | https://lipidomicssociety.org/interest_groups/clinical-lipidomics/ |
Description | Lipid Maps Spring School (1) |
Form Of Engagement Activity | A broadcast e.g. TV/radio/film/podcast (other than news/press) |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Postgraduate students |
Results and Impact | >200 researchers from across World attended the On-line Spring School in real time. All lectures are available on YouTube. |
Year(s) Of Engagement Activity | 2021 |
URL | https://www.youtube.com/watch?v=Zq-wRW_8tyI&list=PLftrKvk5gjt4CKLgC1CZaVyznBksh4O-j&index=3 |
Description | Lipid Maps Spring School (2) |
Form Of Engagement Activity | A broadcast e.g. TV/radio/film/podcast (other than news/press) |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Postgraduate students |
Results and Impact | >200 participants attended the School in real time. The lectures are available on YouTube |
Year(s) Of Engagement Activity | 2021 |
URL | https://www.youtube.com/watch?v=xkumM1PRwHY&list=PLftrKvk5gjt6e_0u4g3otB2Bg_J0LAXy1&index=1 |
Description | Lipid Maps Webinar |
Form Of Engagement Activity | A broadcast e.g. TV/radio/film/podcast (other than news/press) |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Postgraduate students |
Results and Impact | I give a webinar discussing the good and bad side of cholesterol. Over 200 attended live. The presentation is available on YouTube. |
Year(s) Of Engagement Activity | 2020 |
URL | https://www.lipidmaps.org/resources/tutorials/webinars/lipidmaps/sterols_griffiths.php |
Description | RAREsummit23 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Patients, carers and/or patient groups |
Results and Impact | Panellist in the session "DIAGNOSTIC ADVANCES: BEYOND THE GENOME" at the beginning of the meeting. Sparked an interest in the concept of using metabolomics/lipidomics to diagnose rare diseases. |
Year(s) Of Engagement Activity | 2023 |
URL | https://www.youtube.com/watch?v=KsZ4PvELAec |
Description | Schroepfer Medal |
Form Of Engagement Activity | A broadcast e.g. TV/radio/film/podcast (other than news/press) |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | Award Lecture to the American Oil Chemist's Society. Lecture is available on YouTube. |
Year(s) Of Engagement Activity | 2021 |
URL | https://www.youtube.com/watch?v=J9TCRUHmNGg |