National Biofilms Innovation Centre NBIC Flexible Talent Mobility Account

Lead Research Organisation: University of Southampton
Department Name: Sch of Biological Sciences

Abstract

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Organisations

Publications

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Townsend EM (2020) CAUTI's next top model - Model dependent Klebsiella biofilm inhibition by bacteriophages and antimicrobials. in Biofilm

 
Description The award is very attractive for inter academic and industry collaborations. NBIC was able to award 34 quality projects for placements and fellowships. Half of them have completed despite of the hardships of the Pandemic. Successful results included further funding, publication, and job creation. Scientific accomplishment include biological solutions for green and clean production and energy saving methodology, by collaboration between biocoating researcher from University of Surrey and a biological solutions company working together to make waste water treatment less energy intensive by introducing specific biocoating for wastewater treatments facilities. The collaboration is exploring further development of the project into actual products and industrial applications.
Exploitation Route The award was very well received across NBIC's wide network of researchers and companies, and led to efficient pairing of unmet industry needs with research expertise.
Sectors Agriculture, Food and Drink,Chemicals,Education,Energy,Environment,Healthcare,Manufacturing, including Industrial Biotechology,Pharmaceuticals and Medical Biotechnology
URL http://www.biofilms.ac.uk
 
Description The impacts from this FTMA award are all reported in the return for BBSRC NBIC (BB/R012415/1)
Sector Agriculture, Food and Drink,Energy,Environment,Healthcare,Manufacturing, including Industrial Biotechology,Pharmaceuticals and Medical Biotechnology,Transport
Impact Types Societal,Economic
 
Description Screening method (Claudio Lourenco)
Geographic Reach Multiple continents/international 
Policy Influence Type Influenced training of practitioners or researchers
 
Description SUrfaCe Characteristics Enabled StrategieS against virus transmission (SUCCESS)
Amount £649,501 (GBP)
Funding ID EP/V029762/1 
Organisation Engineering and Physical Sciences Research Council (EPSRC) 
Sector Public
Country United Kingdom
Start 09/2020 
End 03/2022
 
Title Model reactor and culture system for biofilm metrology studies. 
Description We have developed a CDC reactor model system for Pseudomonas aeruginosa culture in order to carry out studies of biofilm reproducibility in partnership with LGC. 
Type Of Material Technology assay or reagent 
Year Produced 2022 
Provided To Others? No  
Impact Knowledge exchange and collaborative work in the area of biofilm metrology with industry partner LGC. 
 
Title Using H-NMR to detect the generation of Reactive Oxygen Species (Claudio Lourenco) 
Description This technique consists in the use of H-NMR to follow the reactions taking place within a complex formulation with particular emphasis in the generation of ROS. On its own the technique can effectively detect and quantify the elements present in the mixture. By fine tuning the pH of the environment the generation of ROS can be increased and its half-life in solution increased. 
Type Of Material Technology assay or reagent 
Year Produced 2017 
Provided To Others? Yes  
Impact The main impact is that it allows a quick screening and quantification of a complex mixture of ingredients within a solution. It can be extremely valuable to compare new formulations enabling the researcher to test the reactants efficacy in the early stages of product development saving costs and time on not so good formulations. 
 
Title Workflow for genomic assessment of microbially influenced corrosion 
Description The team have developed expertise in field based DNA sequencing and the use of the Nanopore sequencing platform for energy sector samples. Furthermore, the secondment has facilitate the development of industry links, both in the UK and internationally through DNV GL's global research team. 
Type Of Material Technology assay or reagent 
Year Produced 2023 
Provided To Others? No  
Impact The FTMA application has allowed knowledge exchange between NBIC researchers and renewable energy industries and infrastructure that will be required for the energy transition. This has involved working with DNV who provide access to bacterial corrosion samples, and have assets such as wind farms and pipeline digs and are who are familiar with the industries standardisation processes. 
 
Title YouSeq The ONE 16S NGS kit 
Description Create a ready to sequence 16S Library in one closed tube reaction. The ONE 16S NGS kit contains all of the reagents necessary to create a ready-to-sequence NGS library in minutes. In a breakthrough kit format, the user simply performs one closed tube qPCR reaction. The variable regions V3/4 are targeted, amplified and adapters are added in a single reaction. The quantitative PCR read out simultaneously quantifies each library so they can be pooled precisely. Then a simple bead-clean completes the workflow. After sequencing, the data can then be loaded on to our cloud for rapid analysis. A detailed report is typically returned within 15 minutes. 
Type Of Material Technology assay or reagent 
Year Produced 2019 
Provided To Others? Yes  
Impact This product was developed during an NBIC funded secondment. 
URL https://youseq.com/product/the-one-16s-ngs-kit/8
 
Description Clinical collaboration with Salford Royal NHS Foundation Trust (Mohamed El Mohtadi) 
Organisation Manchester Metropolitan University
Country United Kingdom 
Sector Academic/University 
PI Contribution Securing the FTMA has allowed me to strengthen my collaboration with 5D Health Protection Group Ltd and Manchester Metropolitan University which resulted in me obtaining a Visiting Lecturer position at MMU. The in vitro work carried out during the fellowship has attracted clinical collaborations as we are now replicating the experiments using clinical samples obtained from Salford Royal NHS Foundation Trust operated Salford Royal Hospital.
Collaborator Contribution The role of collaborators (i.e. Dr Ashworth) involves the conceptualisation of project ideas and supervision of students. This will ultimately lead to increased research outcomes and the publication of several original research articles in the near future.
Impact Submission of a grant bid to the Academy of Medical Sciences. The application is currently under review.
Start Year 2020
 
Description Clinical collaboration with Salford Royal NHS Foundation Trust (Mohamed El Mohtadi) 
Organisation Salford Royal NHS Foundation Trust
Country United Kingdom 
Sector Public 
PI Contribution Securing the FTMA has allowed me to strengthen my collaboration with 5D Health Protection Group Ltd and Manchester Metropolitan University which resulted in me obtaining a Visiting Lecturer position at MMU. The in vitro work carried out during the fellowship has attracted clinical collaborations as we are now replicating the experiments using clinical samples obtained from Salford Royal NHS Foundation Trust operated Salford Royal Hospital.
Collaborator Contribution The role of collaborators (i.e. Dr Ashworth) involves the conceptualisation of project ideas and supervision of students. This will ultimately lead to increased research outcomes and the publication of several original research articles in the near future.
Impact Submission of a grant bid to the Academy of Medical Sciences. The application is currently under review.
Start Year 2020
 
Description DNV-GL Industrial Partnership 
Organisation DNV GL
Country Norway 
Sector Private 
PI Contribution Expertise in field based microbial genome sequencing
Collaborator Contribution Access to industrial sampling sites
Impact No publication outputs yet
Start Year 2020
 
Description Impact Acceleration Account 2020-22: Bioinspired Antimicrobial Surfaces (Andrew Parnell) 
Organisation Engineering and Physical Sciences Research Council (EPSRC)
Country United Kingdom 
Sector Public 
PI Contribution EPSRC provided funding for this project which is a continuation of work from the NBIC funded FTMA project (reference FTMA_20_IF_070).
Collaborator Contribution EPSRC provided funding for this project which is a continuation of work from the NBIC funded FTMA project (reference FTMA_20_IF_070).
Impact EPSRC provided funding for this project which is a continuation of work from the NBIC funded FTMA project (reference FTMA_20_IF_070).
Start Year 2021
 
Description Impact Acceleration Account 2020-22: Bioinspired Antimicrobial Surfaces (Andrew Parnell) 
Organisation University of Sheffield
Country United Kingdom 
Sector Academic/University 
PI Contribution EPSRC provided funding for this project which is a continuation of work from the NBIC funded FTMA project (reference FTMA_20_IF_070).
Collaborator Contribution EPSRC provided funding for this project which is a continuation of work from the NBIC funded FTMA project (reference FTMA_20_IF_070).
Impact EPSRC provided funding for this project which is a continuation of work from the NBIC funded FTMA project (reference FTMA_20_IF_070).
Start Year 2021
 
Description Industry partnership with LGC 
Organisation Laboratory of the Government Chemist (LGC) Ltd
Country United Kingdom 
Sector Private 
PI Contribution LGC Group, formerly the Laboratory of the Government Chemist, is an international life sciences measurement and tools company. It provides the role and duties of the UK Government Chemist, a statutory role and adviser to the government. We are working together in the metrology of biofilms, to understand the feasibility of developing new standards for molecular and 'omics approaches to measuring biofilms. We are providing the biofilm models and culture systems in order to generate biological material for the studies.
Collaborator Contribution LGC are applying in-house analytical measurements and approaches in order to understand the variability and reproducibility of biofilm assays, and the feasibility to develop new standards.
Impact Knowledge exchange in use of the CDC biofilm reactor model and in biofilm metrology.
Start Year 2021
 
Description Intelligent Imaging Innovations, Inc. (3i) 
Organisation Intelligent Imaging Innovations Ltd
Country United Kingdom 
Sector Private 
PI Contribution As a result of the BBSRC/NBIC funding (NBIC_FTMA_P_19_2_47 and NBIC_FTMA_20_IF_083) we have established a working relationship with 3i and now have a NBIC DTP iCASE studentship that will take forward promising results from these pump priming projects.
Collaborator Contribution 3i will provide access to advanced instrumentation and placment training for the iCASE student
Impact None to date
Start Year 2023
 
Description NBIC FTMA Fellowship 20_IF_070 Bioinspired Antimicrobial Surfaces (Andrew Parnell) 
Organisation Farapack Polymers
Country United Kingdom 
Sector Private 
PI Contribution Cicada wing surfaces [a] use some as yet unknown physical-mechanical property of the wing surface nanostructure to make them bactericidal. The surface nanostructure is actively killing the bacteria, without the release of a chemical, possibly by puncturing the Bacterial wall. We plan to use this same physical mechanism as a way to kill bacteria and limit the spread of bacteria. Our approach is to use low cost plastic materials that are patterned with a similar surface as is on the cicada. We have already made suitable nano-patterned negative print surfaces in our labs at Sheffield. Our aims in this FTMA fellowship are 1) To test a number of polymer materials as nanostructured surfaces to see if they are effective against colonisation by clinically relevant bacterial strains. This will be using colony forming unit (CFU) counts and live-dead stain fluorescence imaging. This is in collaboration with my colleague Dr Rebecca Corrigan also at Sheffield. Our project is in collaboration with Farapack Polymers Ltd, the PDRA will work with Farapack for the duration of the project. 2) Farapack will help in the development of a prototype nanostructured polymer surface, primarily by helping to screen a number of potential commercial polymer materials that can be manufactured at scale. They have committed £7000 on their side as part of this proof of concept project (see supporting letter), this will involve ongoing development meetings and supply of materials. In later stages they will help by using their extensive existing industrial network to setup and facilitate commercial links. Our project aligns with the NIBIC themes of preventing and managing biofilms, and the themes of transforming foundation industries and leading-edge healthcare. Ivanova, E. P. et al. Natural Bactericidal Surfaces: Mechanical Rupture of Pseudomonas aeruginosa Cells by Cicada Wings. Small 8, 2489-2494 (2012).
Collaborator Contribution We plan to demonstrate the efficacy of antibacterial polymer surfaces that can be made at scale using existing FDA approved materials which mean that this technology could be adopted in a number of clinical settings. We hope to generate IP as one outcome, Dr Parnell has done this before, so has experience of not disclosing information into the public domain along with keeping an audited track of the invention process. The new venture with Farapack will hopefully lead to a number of potential large multinational companies that have the appropriate manufacturing capability to make such a surface. Dr Parnell is currently preparing a UKRI future leaders fellowship in the area of bio-inspired technologies. Studying the mechanism underlying bacterial death on nano-structured surfaces from a fundamental level forms a major part of the fellowship. Publications will stem from a number of different aspects: 1) Firstly understanding the mechanism of action on cicada wings, this will involve a series of in-situ experiments constantly monitoring the membrane and lipid layers. Using naturally fluorescent bacteria will make it possible to see if there is a single burst response or if the mechanism is more subtle. 2) Replication of the biological nanostructure on optimised nano-structured polymer surfaces, provided that we have first patent protected a number of key findings that will help to validate how these surfaces work when they interact with bacteria along with how to optimise them further.
Impact We now have a much better insight into how polymer stiffness plays a role in modulating the killing efficiency. It is an important parameter that must be optimised by the appropriate choice of polymer used to make the nanostructured surface. The link between stiffness and can alter the killing efficiency from near ~ 50-60% down to 20%. This knowledge is important and has helped the team to open discussions with suitable potential commercial partners about this work with the aim of taking it from the lab to a more technology ready state and hopefully to becoming a product. Our discussions with companies are ongoing and may lead to further funding or testing of specific materials. We have also received Impact acceleration account (IAA) funding to further progress the work, this is via the University of Sheffield. Dr Parnell has also applied for a UKRI future leaders fellowship (round 6) part of which concerns further development and commercialisation of antibacterial nanostructured surfaces, this will be decided upon by Jan/Feb 2022. The team also plan to apply for subsequent funding from either the MRC or the BBSRC. Subject to IP considerations we intend to publish this work and will give credit to the NBIC funding for its pivotal role in helping us to understand the role of materials mechanics in making more effective antibacterial surfaces.
Start Year 2021
 
Description NBIC FTMA Fellowship 20_IF_070 Bioinspired Antimicrobial Surfaces (Andrew Parnell) 
Organisation National Biofilms Innovation Centre
Sector Private 
PI Contribution Cicada wing surfaces [a] use some as yet unknown physical-mechanical property of the wing surface nanostructure to make them bactericidal. The surface nanostructure is actively killing the bacteria, without the release of a chemical, possibly by puncturing the Bacterial wall. We plan to use this same physical mechanism as a way to kill bacteria and limit the spread of bacteria. Our approach is to use low cost plastic materials that are patterned with a similar surface as is on the cicada. We have already made suitable nano-patterned negative print surfaces in our labs at Sheffield. Our aims in this FTMA fellowship are 1) To test a number of polymer materials as nanostructured surfaces to see if they are effective against colonisation by clinically relevant bacterial strains. This will be using colony forming unit (CFU) counts and live-dead stain fluorescence imaging. This is in collaboration with my colleague Dr Rebecca Corrigan also at Sheffield. Our project is in collaboration with Farapack Polymers Ltd, the PDRA will work with Farapack for the duration of the project. 2) Farapack will help in the development of a prototype nanostructured polymer surface, primarily by helping to screen a number of potential commercial polymer materials that can be manufactured at scale. They have committed £7000 on their side as part of this proof of concept project (see supporting letter), this will involve ongoing development meetings and supply of materials. In later stages they will help by using their extensive existing industrial network to setup and facilitate commercial links. Our project aligns with the NIBIC themes of preventing and managing biofilms, and the themes of transforming foundation industries and leading-edge healthcare. Ivanova, E. P. et al. Natural Bactericidal Surfaces: Mechanical Rupture of Pseudomonas aeruginosa Cells by Cicada Wings. Small 8, 2489-2494 (2012).
Collaborator Contribution We plan to demonstrate the efficacy of antibacterial polymer surfaces that can be made at scale using existing FDA approved materials which mean that this technology could be adopted in a number of clinical settings. We hope to generate IP as one outcome, Dr Parnell has done this before, so has experience of not disclosing information into the public domain along with keeping an audited track of the invention process. The new venture with Farapack will hopefully lead to a number of potential large multinational companies that have the appropriate manufacturing capability to make such a surface. Dr Parnell is currently preparing a UKRI future leaders fellowship in the area of bio-inspired technologies. Studying the mechanism underlying bacterial death on nano-structured surfaces from a fundamental level forms a major part of the fellowship. Publications will stem from a number of different aspects: 1) Firstly understanding the mechanism of action on cicada wings, this will involve a series of in-situ experiments constantly monitoring the membrane and lipid layers. Using naturally fluorescent bacteria will make it possible to see if there is a single burst response or if the mechanism is more subtle. 2) Replication of the biological nanostructure on optimised nano-structured polymer surfaces, provided that we have first patent protected a number of key findings that will help to validate how these surfaces work when they interact with bacteria along with how to optimise them further.
Impact We now have a much better insight into how polymer stiffness plays a role in modulating the killing efficiency. It is an important parameter that must be optimised by the appropriate choice of polymer used to make the nanostructured surface. The link between stiffness and can alter the killing efficiency from near ~ 50-60% down to 20%. This knowledge is important and has helped the team to open discussions with suitable potential commercial partners about this work with the aim of taking it from the lab to a more technology ready state and hopefully to becoming a product. Our discussions with companies are ongoing and may lead to further funding or testing of specific materials. We have also received Impact acceleration account (IAA) funding to further progress the work, this is via the University of Sheffield. Dr Parnell has also applied for a UKRI future leaders fellowship (round 6) part of which concerns further development and commercialisation of antibacterial nanostructured surfaces, this will be decided upon by Jan/Feb 2022. The team also plan to apply for subsequent funding from either the MRC or the BBSRC. Subject to IP considerations we intend to publish this work and will give credit to the NBIC funding for its pivotal role in helping us to understand the role of materials mechanics in making more effective antibacterial surfaces.
Start Year 2021
 
Description NBIC FTMA Fellowship 20_IF_070 Bioinspired Antimicrobial Surfaces (Andrew Parnell) 
Organisation University of Sheffield
Country United Kingdom 
Sector Academic/University 
PI Contribution Cicada wing surfaces [a] use some as yet unknown physical-mechanical property of the wing surface nanostructure to make them bactericidal. The surface nanostructure is actively killing the bacteria, without the release of a chemical, possibly by puncturing the Bacterial wall. We plan to use this same physical mechanism as a way to kill bacteria and limit the spread of bacteria. Our approach is to use low cost plastic materials that are patterned with a similar surface as is on the cicada. We have already made suitable nano-patterned negative print surfaces in our labs at Sheffield. Our aims in this FTMA fellowship are 1) To test a number of polymer materials as nanostructured surfaces to see if they are effective against colonisation by clinically relevant bacterial strains. This will be using colony forming unit (CFU) counts and live-dead stain fluorescence imaging. This is in collaboration with my colleague Dr Rebecca Corrigan also at Sheffield. Our project is in collaboration with Farapack Polymers Ltd, the PDRA will work with Farapack for the duration of the project. 2) Farapack will help in the development of a prototype nanostructured polymer surface, primarily by helping to screen a number of potential commercial polymer materials that can be manufactured at scale. They have committed £7000 on their side as part of this proof of concept project (see supporting letter), this will involve ongoing development meetings and supply of materials. In later stages they will help by using their extensive existing industrial network to setup and facilitate commercial links. Our project aligns with the NIBIC themes of preventing and managing biofilms, and the themes of transforming foundation industries and leading-edge healthcare. Ivanova, E. P. et al. Natural Bactericidal Surfaces: Mechanical Rupture of Pseudomonas aeruginosa Cells by Cicada Wings. Small 8, 2489-2494 (2012).
Collaborator Contribution We plan to demonstrate the efficacy of antibacterial polymer surfaces that can be made at scale using existing FDA approved materials which mean that this technology could be adopted in a number of clinical settings. We hope to generate IP as one outcome, Dr Parnell has done this before, so has experience of not disclosing information into the public domain along with keeping an audited track of the invention process. The new venture with Farapack will hopefully lead to a number of potential large multinational companies that have the appropriate manufacturing capability to make such a surface. Dr Parnell is currently preparing a UKRI future leaders fellowship in the area of bio-inspired technologies. Studying the mechanism underlying bacterial death on nano-structured surfaces from a fundamental level forms a major part of the fellowship. Publications will stem from a number of different aspects: 1) Firstly understanding the mechanism of action on cicada wings, this will involve a series of in-situ experiments constantly monitoring the membrane and lipid layers. Using naturally fluorescent bacteria will make it possible to see if there is a single burst response or if the mechanism is more subtle. 2) Replication of the biological nanostructure on optimised nano-structured polymer surfaces, provided that we have first patent protected a number of key findings that will help to validate how these surfaces work when they interact with bacteria along with how to optimise them further.
Impact We now have a much better insight into how polymer stiffness plays a role in modulating the killing efficiency. It is an important parameter that must be optimised by the appropriate choice of polymer used to make the nanostructured surface. The link between stiffness and can alter the killing efficiency from near ~ 50-60% down to 20%. This knowledge is important and has helped the team to open discussions with suitable potential commercial partners about this work with the aim of taking it from the lab to a more technology ready state and hopefully to becoming a product. Our discussions with companies are ongoing and may lead to further funding or testing of specific materials. We have also received Impact acceleration account (IAA) funding to further progress the work, this is via the University of Sheffield. Dr Parnell has also applied for a UKRI future leaders fellowship (round 6) part of which concerns further development and commercialisation of antibacterial nanostructured surfaces, this will be decided upon by Jan/Feb 2022. The team also plan to apply for subsequent funding from either the MRC or the BBSRC. Subject to IP considerations we intend to publish this work and will give credit to the NBIC funding for its pivotal role in helping us to understand the role of materials mechanics in making more effective antibacterial surfaces.
Start Year 2021
 
Description NBIC FTMA Fellowship 20_IF_074 Engineering synthetic biofilm for improving energy efficiency in wastewater treatment (Yuxiu Chen) 
Organisation National Biofilms Innovation Centre
Sector Private 
PI Contribution The aim of this secondment is to explore the potential application of our newly developed material, called biocoating, in wastewater treatment. Biocoating is an engineered biofilm that consists of a synthetic polymeric matrix combined with encapsulated bacteria. The two major advantages of biocoating are that the bacterial population is controlled and there is good diffusion through the matrix, which potentially offers a solution to some of the challenges in conventional biofilm bioreactors. This project will act as a proof-of-concept study and initiate a potential long-term collaboration with Novozymes, a global leader in providing biological solutions for wastewater treatment. This project offers a potential means to reduce the carbon emissions associated with the wastewater industry by facilitating significant energy savings. Wastewater treatment is responsible for approximately 2% of a country's total electricity consumption with most of this energy used for aeration and solids separation with centrifugation, both of which are related to the biological wastewater treatment process. Therefore, there is considerable interest from the industry to develop and employ new technologies to reduce the overall energy consumption of wastewater treatment. My initial research focused on developing and characterising new biocoating materials. As this progressed, I recognised the potential application for biocoatings in wastewater treatment. However, as our research team consists only of physicists and microbiologists, we do not have a strong connection to the wastewater industry. As the postdoctoral researcher of the team, I am eagerly exploring this application in wastewater treatment by seeking collaboration with a strong industrial partner. This potential collaboration with Novozymes provides a perfect opportunity for me to work with experts in wastewater treatment and understand the state-of-the-art of the industry. The valuable knowledge and potential long-term collaboration gained from this secondment will guide me in my future research in developing biocoatings for real-world applications.
Collaborator Contribution There are two key results that we want to achieve in this three-month project. Firstly, we will, for the first time, encapsulate commercial bacterial products in our biocoating. Previously, we have only had experience with encapsulating E.coli, which acted as a model system for our research. In this project, mixed-culture bacteria that are commercially used for wastewater treatment bioaugmentation will be encapsulated. The bacteria will be provided by Novozymes. This would represent a huge step in the research of our biocoating as it would represent not only the application of commercially interesting bacteria, but would also provide experience around the viability of mixed culture communities in the biocoating. The viability and reactivity of the bacteria in the biocoating will be a key measure for this part of the study, which will be characterised both in our lab and in Novozymes' lab. Secondly, we will coat our biocoatings on large substrates that can be tested directly in a lab-scale bioreactor. The bioreactor used for this study will be a 1 L lab-scale model of a rotating biological contactor (RBC) in Novozymes' lab. The reason RBC is chosen for this study is because it is commercially available, easily scalable and most importantly, energy efficient (as no aeration is needed). Fibrous substrates will be used to provide good diffusion from both sides of our biocoatings. Disc-shape substrates will be coated with biocoatings in our lab and shipped to Novozymes' lab for testing the reactivity of the encapsulated bacterial products using RBC. In this three-month period of collaboration, Novozymes will share their knowledge on wastewater treatment as well as their lab facilities for testing the reactivity of our biocoatings. We will share our knowledge on bacteria encapsulation and prepare samples for them for testing. We will maintain regular contact through video-meetings. I plan to visit Novozymes' lab for demonstrations and discussions, provided that the Covid pandemic eases before the end of the project. If the results of this proof-of-concept study are promising, that is to say our biocoating has a reasonable reactivity when tested in the RBC, Novozymes will be very likely to establish a long-term collaboration with us to further investigate the application of biocoating in wastewater treatment. While an agreement is still under discussion, it is also possible that we will be able develop collaborative IP and publish this work in a relevant journal if the results are positive.
Impact Feedback from University of Surrey: 1. The project team from Surrey and Novozymes met bi-weekly to track progress and to make plans. Novozymes made two shipments of Prawnbac bacteria to the Surrey labs. We learnt from Novozymes their standard procedure of characterising the reactivity of the nitrifying bacteria (in suspension). We adapted this procedure for characterising the reactivity of nitrifying bacteria immobilised in our biocoatings. Dr Chen built a small lab-scale bioreactor, which he used to study the reactivity of our biocoatings. Using the bacteria and characterisation method provided by Novozymes, he characterised the biocoatings according to an industrial standard. 2. Using the procedure described above, Dr Chen characterised the reactivity of biocoatings fabricated with six different combinations of porous substrates and film formation processes. One trial with carbon paper substrates and a short film formation time showed promising reactivity (181 mg NH4-N produced/L/h). SEM study showed that bacteria were embedded inside the biocoatings after the film formation. Once rehydrated, these embedded bacteria acted as anchoring/initiation sites for natural biofilm growth on the surface of the biocoatings (Figure 1). This study validated the concept of applying our biocoatings to immobilise a commercial nitrifier and opened up possibilities for future commercial biocoating bioreactors. The potential benefits of biocoating bioreactors include a shortened process start-up stage, protection against the failure of biofilms, protection of sensitive nitrifier populations to inhibition, and the potential to intensify wastewater nitrification processes, all of which are of great interest to Novozymes. 3. We realised that the poor desiccation tolerance of Prawnbac limited the survivability of bacteria during the film formation process of the biocoating, which is conventionally a desiccation process. We proposed a new concept of film formation via the method of "wet sintering", which does not require desiccation during polymer particle coalescence as part of the film formation process. This proof-of-concept experiment started near the end of this NBIC fellowship, and is still ongoing at the time this report is being written. Our collaborator from Novozymes was impressed by the versatility of our biocoatings and the ability we have to adapt our biocoatings. 4. Novozymes has gained a better insight into the characteristics of their own product and how it responds under the conditions of film formation. They had not previously performed scanning electron microscopy on their product and its biofilm. Hence, the characterisation gave them a valuable insight. Next steps: We will continue our collaboration with Novozymes, to study our biocoatings using the bacteria Novozymes provided us. We plan to ship biocoatings from Surrey to Novozymes for them to analyse in their application, using specialised microbial analysis methods such as FISH and qPCR. Our current focus is to develop our concept of film formation of biocoatings without desiccation, which aligns perfectly with our ongoing project on biocoatings and fits our expertise as materials physicists. Novozymes also shows interest in this concept, as it offers a unique solution to immobilise their desiccation sensitive bacteria. We will apply for grants together with Novozymes to continue our research on this project. We have identified the Novo Nordisk Foundation as a possible source of follow-on funding. An alternative source of funding is through the BBSRC Engineering Biology programme.
Start Year 2021
 
Description NBIC FTMA Fellowship 20_IF_074 Engineering synthetic biofilm for improving energy efficiency in wastewater treatment (Yuxiu Chen) 
Organisation Novozymes
Country Denmark 
Sector Public 
PI Contribution The aim of this secondment is to explore the potential application of our newly developed material, called biocoating, in wastewater treatment. Biocoating is an engineered biofilm that consists of a synthetic polymeric matrix combined with encapsulated bacteria. The two major advantages of biocoating are that the bacterial population is controlled and there is good diffusion through the matrix, which potentially offers a solution to some of the challenges in conventional biofilm bioreactors. This project will act as a proof-of-concept study and initiate a potential long-term collaboration with Novozymes, a global leader in providing biological solutions for wastewater treatment. This project offers a potential means to reduce the carbon emissions associated with the wastewater industry by facilitating significant energy savings. Wastewater treatment is responsible for approximately 2% of a country's total electricity consumption with most of this energy used for aeration and solids separation with centrifugation, both of which are related to the biological wastewater treatment process. Therefore, there is considerable interest from the industry to develop and employ new technologies to reduce the overall energy consumption of wastewater treatment. My initial research focused on developing and characterising new biocoating materials. As this progressed, I recognised the potential application for biocoatings in wastewater treatment. However, as our research team consists only of physicists and microbiologists, we do not have a strong connection to the wastewater industry. As the postdoctoral researcher of the team, I am eagerly exploring this application in wastewater treatment by seeking collaboration with a strong industrial partner. This potential collaboration with Novozymes provides a perfect opportunity for me to work with experts in wastewater treatment and understand the state-of-the-art of the industry. The valuable knowledge and potential long-term collaboration gained from this secondment will guide me in my future research in developing biocoatings for real-world applications.
Collaborator Contribution There are two key results that we want to achieve in this three-month project. Firstly, we will, for the first time, encapsulate commercial bacterial products in our biocoating. Previously, we have only had experience with encapsulating E.coli, which acted as a model system for our research. In this project, mixed-culture bacteria that are commercially used for wastewater treatment bioaugmentation will be encapsulated. The bacteria will be provided by Novozymes. This would represent a huge step in the research of our biocoating as it would represent not only the application of commercially interesting bacteria, but would also provide experience around the viability of mixed culture communities in the biocoating. The viability and reactivity of the bacteria in the biocoating will be a key measure for this part of the study, which will be characterised both in our lab and in Novozymes' lab. Secondly, we will coat our biocoatings on large substrates that can be tested directly in a lab-scale bioreactor. The bioreactor used for this study will be a 1 L lab-scale model of a rotating biological contactor (RBC) in Novozymes' lab. The reason RBC is chosen for this study is because it is commercially available, easily scalable and most importantly, energy efficient (as no aeration is needed). Fibrous substrates will be used to provide good diffusion from both sides of our biocoatings. Disc-shape substrates will be coated with biocoatings in our lab and shipped to Novozymes' lab for testing the reactivity of the encapsulated bacterial products using RBC. In this three-month period of collaboration, Novozymes will share their knowledge on wastewater treatment as well as their lab facilities for testing the reactivity of our biocoatings. We will share our knowledge on bacteria encapsulation and prepare samples for them for testing. We will maintain regular contact through video-meetings. I plan to visit Novozymes' lab for demonstrations and discussions, provided that the Covid pandemic eases before the end of the project. If the results of this proof-of-concept study are promising, that is to say our biocoating has a reasonable reactivity when tested in the RBC, Novozymes will be very likely to establish a long-term collaboration with us to further investigate the application of biocoating in wastewater treatment. While an agreement is still under discussion, it is also possible that we will be able develop collaborative IP and publish this work in a relevant journal if the results are positive.
Impact Feedback from University of Surrey: 1. The project team from Surrey and Novozymes met bi-weekly to track progress and to make plans. Novozymes made two shipments of Prawnbac bacteria to the Surrey labs. We learnt from Novozymes their standard procedure of characterising the reactivity of the nitrifying bacteria (in suspension). We adapted this procedure for characterising the reactivity of nitrifying bacteria immobilised in our biocoatings. Dr Chen built a small lab-scale bioreactor, which he used to study the reactivity of our biocoatings. Using the bacteria and characterisation method provided by Novozymes, he characterised the biocoatings according to an industrial standard. 2. Using the procedure described above, Dr Chen characterised the reactivity of biocoatings fabricated with six different combinations of porous substrates and film formation processes. One trial with carbon paper substrates and a short film formation time showed promising reactivity (181 mg NH4-N produced/L/h). SEM study showed that bacteria were embedded inside the biocoatings after the film formation. Once rehydrated, these embedded bacteria acted as anchoring/initiation sites for natural biofilm growth on the surface of the biocoatings (Figure 1). This study validated the concept of applying our biocoatings to immobilise a commercial nitrifier and opened up possibilities for future commercial biocoating bioreactors. The potential benefits of biocoating bioreactors include a shortened process start-up stage, protection against the failure of biofilms, protection of sensitive nitrifier populations to inhibition, and the potential to intensify wastewater nitrification processes, all of which are of great interest to Novozymes. 3. We realised that the poor desiccation tolerance of Prawnbac limited the survivability of bacteria during the film formation process of the biocoating, which is conventionally a desiccation process. We proposed a new concept of film formation via the method of "wet sintering", which does not require desiccation during polymer particle coalescence as part of the film formation process. This proof-of-concept experiment started near the end of this NBIC fellowship, and is still ongoing at the time this report is being written. Our collaborator from Novozymes was impressed by the versatility of our biocoatings and the ability we have to adapt our biocoatings. 4. Novozymes has gained a better insight into the characteristics of their own product and how it responds under the conditions of film formation. They had not previously performed scanning electron microscopy on their product and its biofilm. Hence, the characterisation gave them a valuable insight. Next steps: We will continue our collaboration with Novozymes, to study our biocoatings using the bacteria Novozymes provided us. We plan to ship biocoatings from Surrey to Novozymes for them to analyse in their application, using specialised microbial analysis methods such as FISH and qPCR. Our current focus is to develop our concept of film formation of biocoatings without desiccation, which aligns perfectly with our ongoing project on biocoatings and fits our expertise as materials physicists. Novozymes also shows interest in this concept, as it offers a unique solution to immobilise their desiccation sensitive bacteria. We will apply for grants together with Novozymes to continue our research on this project. We have identified the Novo Nordisk Foundation as a possible source of follow-on funding. An alternative source of funding is through the BBSRC Engineering Biology programme.
Start Year 2021
 
Description NBIC FTMA Fellowship 20_IF_074 Engineering synthetic biofilm for improving energy efficiency in wastewater treatment (Yuxiu Chen) 
Organisation University of Surrey
Country United Kingdom 
Sector Academic/University 
PI Contribution The aim of this secondment is to explore the potential application of our newly developed material, called biocoating, in wastewater treatment. Biocoating is an engineered biofilm that consists of a synthetic polymeric matrix combined with encapsulated bacteria. The two major advantages of biocoating are that the bacterial population is controlled and there is good diffusion through the matrix, which potentially offers a solution to some of the challenges in conventional biofilm bioreactors. This project will act as a proof-of-concept study and initiate a potential long-term collaboration with Novozymes, a global leader in providing biological solutions for wastewater treatment. This project offers a potential means to reduce the carbon emissions associated with the wastewater industry by facilitating significant energy savings. Wastewater treatment is responsible for approximately 2% of a country's total electricity consumption with most of this energy used for aeration and solids separation with centrifugation, both of which are related to the biological wastewater treatment process. Therefore, there is considerable interest from the industry to develop and employ new technologies to reduce the overall energy consumption of wastewater treatment. My initial research focused on developing and characterising new biocoating materials. As this progressed, I recognised the potential application for biocoatings in wastewater treatment. However, as our research team consists only of physicists and microbiologists, we do not have a strong connection to the wastewater industry. As the postdoctoral researcher of the team, I am eagerly exploring this application in wastewater treatment by seeking collaboration with a strong industrial partner. This potential collaboration with Novozymes provides a perfect opportunity for me to work with experts in wastewater treatment and understand the state-of-the-art of the industry. The valuable knowledge and potential long-term collaboration gained from this secondment will guide me in my future research in developing biocoatings for real-world applications.
Collaborator Contribution There are two key results that we want to achieve in this three-month project. Firstly, we will, for the first time, encapsulate commercial bacterial products in our biocoating. Previously, we have only had experience with encapsulating E.coli, which acted as a model system for our research. In this project, mixed-culture bacteria that are commercially used for wastewater treatment bioaugmentation will be encapsulated. The bacteria will be provided by Novozymes. This would represent a huge step in the research of our biocoating as it would represent not only the application of commercially interesting bacteria, but would also provide experience around the viability of mixed culture communities in the biocoating. The viability and reactivity of the bacteria in the biocoating will be a key measure for this part of the study, which will be characterised both in our lab and in Novozymes' lab. Secondly, we will coat our biocoatings on large substrates that can be tested directly in a lab-scale bioreactor. The bioreactor used for this study will be a 1 L lab-scale model of a rotating biological contactor (RBC) in Novozymes' lab. The reason RBC is chosen for this study is because it is commercially available, easily scalable and most importantly, energy efficient (as no aeration is needed). Fibrous substrates will be used to provide good diffusion from both sides of our biocoatings. Disc-shape substrates will be coated with biocoatings in our lab and shipped to Novozymes' lab for testing the reactivity of the encapsulated bacterial products using RBC. In this three-month period of collaboration, Novozymes will share their knowledge on wastewater treatment as well as their lab facilities for testing the reactivity of our biocoatings. We will share our knowledge on bacteria encapsulation and prepare samples for them for testing. We will maintain regular contact through video-meetings. I plan to visit Novozymes' lab for demonstrations and discussions, provided that the Covid pandemic eases before the end of the project. If the results of this proof-of-concept study are promising, that is to say our biocoating has a reasonable reactivity when tested in the RBC, Novozymes will be very likely to establish a long-term collaboration with us to further investigate the application of biocoating in wastewater treatment. While an agreement is still under discussion, it is also possible that we will be able develop collaborative IP and publish this work in a relevant journal if the results are positive.
Impact Feedback from University of Surrey: 1. The project team from Surrey and Novozymes met bi-weekly to track progress and to make plans. Novozymes made two shipments of Prawnbac bacteria to the Surrey labs. We learnt from Novozymes their standard procedure of characterising the reactivity of the nitrifying bacteria (in suspension). We adapted this procedure for characterising the reactivity of nitrifying bacteria immobilised in our biocoatings. Dr Chen built a small lab-scale bioreactor, which he used to study the reactivity of our biocoatings. Using the bacteria and characterisation method provided by Novozymes, he characterised the biocoatings according to an industrial standard. 2. Using the procedure described above, Dr Chen characterised the reactivity of biocoatings fabricated with six different combinations of porous substrates and film formation processes. One trial with carbon paper substrates and a short film formation time showed promising reactivity (181 mg NH4-N produced/L/h). SEM study showed that bacteria were embedded inside the biocoatings after the film formation. Once rehydrated, these embedded bacteria acted as anchoring/initiation sites for natural biofilm growth on the surface of the biocoatings (Figure 1). This study validated the concept of applying our biocoatings to immobilise a commercial nitrifier and opened up possibilities for future commercial biocoating bioreactors. The potential benefits of biocoating bioreactors include a shortened process start-up stage, protection against the failure of biofilms, protection of sensitive nitrifier populations to inhibition, and the potential to intensify wastewater nitrification processes, all of which are of great interest to Novozymes. 3. We realised that the poor desiccation tolerance of Prawnbac limited the survivability of bacteria during the film formation process of the biocoating, which is conventionally a desiccation process. We proposed a new concept of film formation via the method of "wet sintering", which does not require desiccation during polymer particle coalescence as part of the film formation process. This proof-of-concept experiment started near the end of this NBIC fellowship, and is still ongoing at the time this report is being written. Our collaborator from Novozymes was impressed by the versatility of our biocoatings and the ability we have to adapt our biocoatings. 4. Novozymes has gained a better insight into the characteristics of their own product and how it responds under the conditions of film formation. They had not previously performed scanning electron microscopy on their product and its biofilm. Hence, the characterisation gave them a valuable insight. Next steps: We will continue our collaboration with Novozymes, to study our biocoatings using the bacteria Novozymes provided us. We plan to ship biocoatings from Surrey to Novozymes for them to analyse in their application, using specialised microbial analysis methods such as FISH and qPCR. Our current focus is to develop our concept of film formation of biocoatings without desiccation, which aligns perfectly with our ongoing project on biocoatings and fits our expertise as materials physicists. Novozymes also shows interest in this concept, as it offers a unique solution to immobilise their desiccation sensitive bacteria. We will apply for grants together with Novozymes to continue our research on this project. We have identified the Novo Nordisk Foundation as a possible source of follow-on funding. An alternative source of funding is through the BBSRC Engineering Biology programme.
Start Year 2021
 
Description NBIC FTMA Fellowship 20_IF_079 Development of biofilm inhibition testing to the ASTM standard for implanted medical devices (Vanina Garcia Altamirano) 
Organisation National Biofilms Innovation Centre
Sector Private 
PI Contribution The primary ambition of this project is to develop biofilm testing capabilities in the Medical Technologies Innovation Facility (MTIF) to develop their portfolio to include standardised biofilm testing services for academics and industrial clients that wish to test developing medical device materials for biofilm inhibition properties to the American Society for Testing and Materials (ASTM) standard for implanted medical devices. Secondary aims are to develop standard operating procedures (SOPs) for several simple biofilm assays that can be utilised by MTIF as further services. This will support my personal development by providing experience of grant capture as well as working in the industrial sector. This fellowship will develop my commercial awareness, through my involvement in setting up a commercial laboratory and SOPs to the ASTM standard. This is experience that is difficult to gain through academic research alone and will mean that I am able to work effectively within these environments and in collaborations with industrial partners in the future, which is a vital part of bridging the gap between fundamental research and real-world application. This project aligns to the challenges "Leading-edge healthcare" and the "Healthy Ageing" outlined in the Industry Strategy Challenge Fund. Aging populations increasingly require implantable medical devices for improved quality of life and in treatment of disease. As biofilm infection is a leading cause of failure of these devices, the development of anti-biofilm medical materials is critical for the improvement of quality life for millions, having standardised biofilm testing services available in the region to test developing medical device materials provides researchers and commercial developers access to reliable and robust testing of new medical device materials to recognised standards without the costs associated with setting this up in-house. Consequently, the manufacture and commercialisation of antibiofilm materials will be expedited by these services.
Collaborator Contribution Biofilm contamination is a leading cause of failure of medical implant devices that costs lives, reduces quality of life and costs millions of pounds globally. Therefore, development of medical implant device materials that can inhibit the formation of biofilms is critical to the efficacy of such devices. There are few facilities that can offer access to robust standardised biofilm testing within the region. Development of this service within MTIF will provide wide ranging impact as manufacturers, developers and academic researchers of implantable medical device materials and other anti-biofilm materials will be able to gain access to these facilities and expertise to test their products to the recognised ASTM standards (e.g. E2562/E3161) at low cost. There is a recognised lack of standardisation of biofilm testing, linking MTIF to NBIC via fellowships such as this ensures that MTIF is situated at the forefront of the biofilm field ensuring that the testing services developed are appropriate, relevant and useful to the wider community of industrial companies and academic researchers that have interests in biofilm testing. This allows MTIF to provide a biofilm testing service that they currently do not have and will allow them to expand their portfolio. Biofilm testing in a CDC Biofilm Reactor to the ASTM standard is a recognised standard for materials testing and will allow MTIF to provide a robust and reliable service. This fellowship provides MTIF with the expertise of an experienced biofilm researcher able to develop biofilm models to ASTM standards. I have over five years of experience in biofilm research, developing models to study biofilm formation of human pathogens and host-pathogen interactions. I am currently working on a Proof of Concept NBIC grant, developing SOPs to produce novel Vascular Access Grafts and subsequent evaluation of grafts coated with antimicrobials to evaluate their antibiofilm efficacy compared to currently commercially available grafts and their potential for future commercialisation. This fellowship will benefit me as a researcher by providing experience of setting up a commercial biofilm inhibition testing laboratory to the ASTM standard, knowledge that can only be acquired in an industrial setting like MTIF, a dual site research and development facility available to industry and academic institutions to support and accelerate the development of innovative medical technologies. This will provide me with the skills and experience of working with industrial partners and working to industrial standards, which will allow me to develop industrial collaborations for future funding applications.
Impact The achievements of this fellowship were as follow: • The development of a robust SOP for biofilm testing in a CDC Reactor to the internationally recognised ASTM standards to test new materials used in the manufacture of medical devices. • The development of an SOP for Salmonella testing from feed material. • The development of an SOP to test Salmonella on industry surfaces (swabs). These three SOPs have helped to extended MTIF's portfolio. This fellowship has supported my personal development by providing experience of grant capture. It also helped to develop my commercial awareness, through my involvement in setting up a commercial laboratory, developing SOPs to industrial standards, and provided me with the skills and experience of working with industrial partners which will allow me to develop industrial collaborations for future funding applications. A standardised biofilm testing was developed to the well-known ASTM standards, a biofilm testing service that MTIF did not have, and now they can provide it to manufacturers, developers and academic researchers of implantable medical device materials and other anti-biofilm materials within the region and at low cost. Additionally, food industries can also benefit with the new SOPs developed during this placement for Salmonella testing in feed material and swabs. For the next steps, MTIF and NTU are fully supportive and seeking opportunities to support applications for Early Career Fellowships, to continue with the development of methodologies to continue transferring my skills to business. In the meantime, it would be beneficial to have an economical support to develop more biofilm testing methodologies to offer more services to the wider community of industrial companies and academic researchers that have interests in biofilm testing in order to have a continuity in my career whilst the application process for fellowships proceeds.
Start Year 2021
 
Description NBIC FTMA Fellowship 20_IF_079 Development of biofilm inhibition testing to the ASTM standard for implanted medical devices (Vanina Garcia Altamirano) 
Organisation Nottingham Trent University
Country United Kingdom 
Sector Academic/University 
PI Contribution The primary ambition of this project is to develop biofilm testing capabilities in the Medical Technologies Innovation Facility (MTIF) to develop their portfolio to include standardised biofilm testing services for academics and industrial clients that wish to test developing medical device materials for biofilm inhibition properties to the American Society for Testing and Materials (ASTM) standard for implanted medical devices. Secondary aims are to develop standard operating procedures (SOPs) for several simple biofilm assays that can be utilised by MTIF as further services. This will support my personal development by providing experience of grant capture as well as working in the industrial sector. This fellowship will develop my commercial awareness, through my involvement in setting up a commercial laboratory and SOPs to the ASTM standard. This is experience that is difficult to gain through academic research alone and will mean that I am able to work effectively within these environments and in collaborations with industrial partners in the future, which is a vital part of bridging the gap between fundamental research and real-world application. This project aligns to the challenges "Leading-edge healthcare" and the "Healthy Ageing" outlined in the Industry Strategy Challenge Fund. Aging populations increasingly require implantable medical devices for improved quality of life and in treatment of disease. As biofilm infection is a leading cause of failure of these devices, the development of anti-biofilm medical materials is critical for the improvement of quality life for millions, having standardised biofilm testing services available in the region to test developing medical device materials provides researchers and commercial developers access to reliable and robust testing of new medical device materials to recognised standards without the costs associated with setting this up in-house. Consequently, the manufacture and commercialisation of antibiofilm materials will be expedited by these services.
Collaborator Contribution Biofilm contamination is a leading cause of failure of medical implant devices that costs lives, reduces quality of life and costs millions of pounds globally. Therefore, development of medical implant device materials that can inhibit the formation of biofilms is critical to the efficacy of such devices. There are few facilities that can offer access to robust standardised biofilm testing within the region. Development of this service within MTIF will provide wide ranging impact as manufacturers, developers and academic researchers of implantable medical device materials and other anti-biofilm materials will be able to gain access to these facilities and expertise to test their products to the recognised ASTM standards (e.g. E2562/E3161) at low cost. There is a recognised lack of standardisation of biofilm testing, linking MTIF to NBIC via fellowships such as this ensures that MTIF is situated at the forefront of the biofilm field ensuring that the testing services developed are appropriate, relevant and useful to the wider community of industrial companies and academic researchers that have interests in biofilm testing. This allows MTIF to provide a biofilm testing service that they currently do not have and will allow them to expand their portfolio. Biofilm testing in a CDC Biofilm Reactor to the ASTM standard is a recognised standard for materials testing and will allow MTIF to provide a robust and reliable service. This fellowship provides MTIF with the expertise of an experienced biofilm researcher able to develop biofilm models to ASTM standards. I have over five years of experience in biofilm research, developing models to study biofilm formation of human pathogens and host-pathogen interactions. I am currently working on a Proof of Concept NBIC grant, developing SOPs to produce novel Vascular Access Grafts and subsequent evaluation of grafts coated with antimicrobials to evaluate their antibiofilm efficacy compared to currently commercially available grafts and their potential for future commercialisation. This fellowship will benefit me as a researcher by providing experience of setting up a commercial biofilm inhibition testing laboratory to the ASTM standard, knowledge that can only be acquired in an industrial setting like MTIF, a dual site research and development facility available to industry and academic institutions to support and accelerate the development of innovative medical technologies. This will provide me with the skills and experience of working with industrial partners and working to industrial standards, which will allow me to develop industrial collaborations for future funding applications.
Impact The achievements of this fellowship were as follow: • The development of a robust SOP for biofilm testing in a CDC Reactor to the internationally recognised ASTM standards to test new materials used in the manufacture of medical devices. • The development of an SOP for Salmonella testing from feed material. • The development of an SOP to test Salmonella on industry surfaces (swabs). These three SOPs have helped to extended MTIF's portfolio. This fellowship has supported my personal development by providing experience of grant capture. It also helped to develop my commercial awareness, through my involvement in setting up a commercial laboratory, developing SOPs to industrial standards, and provided me with the skills and experience of working with industrial partners which will allow me to develop industrial collaborations for future funding applications. A standardised biofilm testing was developed to the well-known ASTM standards, a biofilm testing service that MTIF did not have, and now they can provide it to manufacturers, developers and academic researchers of implantable medical device materials and other anti-biofilm materials within the region and at low cost. Additionally, food industries can also benefit with the new SOPs developed during this placement for Salmonella testing in feed material and swabs. For the next steps, MTIF and NTU are fully supportive and seeking opportunities to support applications for Early Career Fellowships, to continue with the development of methodologies to continue transferring my skills to business. In the meantime, it would be beneficial to have an economical support to develop more biofilm testing methodologies to offer more services to the wider community of industrial companies and academic researchers that have interests in biofilm testing in order to have a continuity in my career whilst the application process for fellowships proceeds.
Start Year 2021
 
Description NBIC FTMA Fellowship 20_IF_083 Rapid, multi-dimension, long term lightsheet imaging of fungal biofilms under conditions of minimal phototoxicity (Campbell Gourlay) 
Organisation Intelligent Imaging Innovations Ltd
Country United Kingdom 
Sector Private 
PI Contribution We seek funds to support a 3 month fellowship to support Dr. Daniel Pentland, who recently completed his PhD, to collaborate with the instrument manufacturing company Intelligent Imaging Innovations (3i). The company produces a unique and highly versatile light-sheet microscope platform, the Marianas lightsheet that Dr. Pentland will use to generate the first 4D, long term fungal biofilms data gathered under conditions of minimal phototoxicity. The fellowship will greatly extend findings obtained from an NBIC funded FTMA award to Dr. Daniel Pentland (02POC19105) who worked with Carin Scientific to successfully establish light-sheet fluorescence microscopy to image fungal biofilms. The development of such imaging technology will allow us to perfect, for the first time, accurate methods for biomarker identification in complex biofilms, an essential step to enable early diagnosis of the many diseases caused by fungal biofilms and provide new insights to enable eradication. The project therefore falls within the Industry Strategy Challenge Fund areas of "Accelerated detection of disease" and "From data to early diagnosis and precision medicine". The fellowship will enable exchange Dr Pentland's of biological skills, technical expertise, background knowledge and experience in using light sheet to image biofilms obtained within the FTMA award at Cairn Scientific with 3i scientists. Dr. Pentland will work with the 3i team, in consultation with Dr Laissue (Essex) to perfect image analysis of fungal biofilm dynamics using this remarkable technique. As Dr. Pentland will be trained on the Marianas Lightsheet system and software at 3i he will develop a deep understanding of industrial application, product design and market demands. He will receive support and technical advice throughout from applications scientist and Marianas™ Lightsheet product manager, Dr Hella Baumann. Dr. Baumann's time and access to 3i's facility are equivalent to an in-kind support in excess of £5,000 in value.
Collaborator Contribution The main output arises from the design, experimental testing and validation of light sheet microscopy methods in a new area of application: The developmental imaging of fungal biofilms are of medical, environmental and industrial relevance but is a research area that is currently unexplored. This fellowship will enable a step change in the imaging of fungal biofilms by coupling the fellows academic expertise with cutting edge advanced low-light fluorescence microscopy knowledge at 3i. An key output is therefore the transfer of knowledge between industry and academia to develop a new area of research, business opportunity and return to UK Plc. A key industrial output of this research will be to test and develop protocols for the Marianas Light Sheet system so that these can be distributed and used by multiple users, within a range of disciplines, to image fungal biofilms without inflicting photodamage. The fellow will work with 3i to develop protocols that can be rapidly adapted to different fungal biofilm applications, thus extending the applicability of this technology to research groups within medical, environmental and industrial fields. The research will allow development of integrated hardware and software control, creating a seamless user experience with comprehensive control of acquisition and rendering, ideal for a multi-user setting. The expected outputs are reliant on the exchange of skills and knowledge between the fellow and 3i scientists and will lead to tangible benefits to both parties.
Impact Di-SPIM light sheet microscopy is ideal for monitoring the development of a dense fungal biofilm. Our approaches will lead to this technology being applied to a new field and opens a new customer base 3i as was planned within this application.
Start Year 2021
 
Description NBIC FTMA Fellowship 20_IF_083 Rapid, multi-dimension, long term lightsheet imaging of fungal biofilms under conditions of minimal phototoxicity (Campbell Gourlay) 
Organisation National Biofilms Innovation Centre
Sector Private 
PI Contribution We seek funds to support a 3 month fellowship to support Dr. Daniel Pentland, who recently completed his PhD, to collaborate with the instrument manufacturing company Intelligent Imaging Innovations (3i). The company produces a unique and highly versatile light-sheet microscope platform, the Marianas lightsheet that Dr. Pentland will use to generate the first 4D, long term fungal biofilms data gathered under conditions of minimal phototoxicity. The fellowship will greatly extend findings obtained from an NBIC funded FTMA award to Dr. Daniel Pentland (02POC19105) who worked with Carin Scientific to successfully establish light-sheet fluorescence microscopy to image fungal biofilms. The development of such imaging technology will allow us to perfect, for the first time, accurate methods for biomarker identification in complex biofilms, an essential step to enable early diagnosis of the many diseases caused by fungal biofilms and provide new insights to enable eradication. The project therefore falls within the Industry Strategy Challenge Fund areas of "Accelerated detection of disease" and "From data to early diagnosis and precision medicine". The fellowship will enable exchange Dr Pentland's of biological skills, technical expertise, background knowledge and experience in using light sheet to image biofilms obtained within the FTMA award at Cairn Scientific with 3i scientists. Dr. Pentland will work with the 3i team, in consultation with Dr Laissue (Essex) to perfect image analysis of fungal biofilm dynamics using this remarkable technique. As Dr. Pentland will be trained on the Marianas Lightsheet system and software at 3i he will develop a deep understanding of industrial application, product design and market demands. He will receive support and technical advice throughout from applications scientist and Marianas™ Lightsheet product manager, Dr Hella Baumann. Dr. Baumann's time and access to 3i's facility are equivalent to an in-kind support in excess of £5,000 in value.
Collaborator Contribution The main output arises from the design, experimental testing and validation of light sheet microscopy methods in a new area of application: The developmental imaging of fungal biofilms are of medical, environmental and industrial relevance but is a research area that is currently unexplored. This fellowship will enable a step change in the imaging of fungal biofilms by coupling the fellows academic expertise with cutting edge advanced low-light fluorescence microscopy knowledge at 3i. An key output is therefore the transfer of knowledge between industry and academia to develop a new area of research, business opportunity and return to UK Plc. A key industrial output of this research will be to test and develop protocols for the Marianas Light Sheet system so that these can be distributed and used by multiple users, within a range of disciplines, to image fungal biofilms without inflicting photodamage. The fellow will work with 3i to develop protocols that can be rapidly adapted to different fungal biofilm applications, thus extending the applicability of this technology to research groups within medical, environmental and industrial fields. The research will allow development of integrated hardware and software control, creating a seamless user experience with comprehensive control of acquisition and rendering, ideal for a multi-user setting. The expected outputs are reliant on the exchange of skills and knowledge between the fellow and 3i scientists and will lead to tangible benefits to both parties.
Impact Di-SPIM light sheet microscopy is ideal for monitoring the development of a dense fungal biofilm. Our approaches will lead to this technology being applied to a new field and opens a new customer base 3i as was planned within this application.
Start Year 2021
 
Description NBIC FTMA Fellowship 20_IF_083 Rapid, multi-dimension, long term lightsheet imaging of fungal biofilms under conditions of minimal phototoxicity (Campbell Gourlay) 
Organisation University of Essex
Country United Kingdom 
Sector Academic/University 
PI Contribution We seek funds to support a 3 month fellowship to support Dr. Daniel Pentland, who recently completed his PhD, to collaborate with the instrument manufacturing company Intelligent Imaging Innovations (3i). The company produces a unique and highly versatile light-sheet microscope platform, the Marianas lightsheet that Dr. Pentland will use to generate the first 4D, long term fungal biofilms data gathered under conditions of minimal phototoxicity. The fellowship will greatly extend findings obtained from an NBIC funded FTMA award to Dr. Daniel Pentland (02POC19105) who worked with Carin Scientific to successfully establish light-sheet fluorescence microscopy to image fungal biofilms. The development of such imaging technology will allow us to perfect, for the first time, accurate methods for biomarker identification in complex biofilms, an essential step to enable early diagnosis of the many diseases caused by fungal biofilms and provide new insights to enable eradication. The project therefore falls within the Industry Strategy Challenge Fund areas of "Accelerated detection of disease" and "From data to early diagnosis and precision medicine". The fellowship will enable exchange Dr Pentland's of biological skills, technical expertise, background knowledge and experience in using light sheet to image biofilms obtained within the FTMA award at Cairn Scientific with 3i scientists. Dr. Pentland will work with the 3i team, in consultation with Dr Laissue (Essex) to perfect image analysis of fungal biofilm dynamics using this remarkable technique. As Dr. Pentland will be trained on the Marianas Lightsheet system and software at 3i he will develop a deep understanding of industrial application, product design and market demands. He will receive support and technical advice throughout from applications scientist and Marianas™ Lightsheet product manager, Dr Hella Baumann. Dr. Baumann's time and access to 3i's facility are equivalent to an in-kind support in excess of £5,000 in value.
Collaborator Contribution The main output arises from the design, experimental testing and validation of light sheet microscopy methods in a new area of application: The developmental imaging of fungal biofilms are of medical, environmental and industrial relevance but is a research area that is currently unexplored. This fellowship will enable a step change in the imaging of fungal biofilms by coupling the fellows academic expertise with cutting edge advanced low-light fluorescence microscopy knowledge at 3i. An key output is therefore the transfer of knowledge between industry and academia to develop a new area of research, business opportunity and return to UK Plc. A key industrial output of this research will be to test and develop protocols for the Marianas Light Sheet system so that these can be distributed and used by multiple users, within a range of disciplines, to image fungal biofilms without inflicting photodamage. The fellow will work with 3i to develop protocols that can be rapidly adapted to different fungal biofilm applications, thus extending the applicability of this technology to research groups within medical, environmental and industrial fields. The research will allow development of integrated hardware and software control, creating a seamless user experience with comprehensive control of acquisition and rendering, ideal for a multi-user setting. The expected outputs are reliant on the exchange of skills and knowledge between the fellow and 3i scientists and will lead to tangible benefits to both parties.
Impact Di-SPIM light sheet microscopy is ideal for monitoring the development of a dense fungal biofilm. Our approaches will lead to this technology being applied to a new field and opens a new customer base 3i as was planned within this application.
Start Year 2021
 
Description NBIC FTMA Fellowship 20_IF_083 Rapid, multi-dimension, long term lightsheet imaging of fungal biofilms under conditions of minimal phototoxicity (Campbell Gourlay) 
Organisation University of Kent
Country United Kingdom 
Sector Academic/University 
PI Contribution We seek funds to support a 3 month fellowship to support Dr. Daniel Pentland, who recently completed his PhD, to collaborate with the instrument manufacturing company Intelligent Imaging Innovations (3i). The company produces a unique and highly versatile light-sheet microscope platform, the Marianas lightsheet that Dr. Pentland will use to generate the first 4D, long term fungal biofilms data gathered under conditions of minimal phototoxicity. The fellowship will greatly extend findings obtained from an NBIC funded FTMA award to Dr. Daniel Pentland (02POC19105) who worked with Carin Scientific to successfully establish light-sheet fluorescence microscopy to image fungal biofilms. The development of such imaging technology will allow us to perfect, for the first time, accurate methods for biomarker identification in complex biofilms, an essential step to enable early diagnosis of the many diseases caused by fungal biofilms and provide new insights to enable eradication. The project therefore falls within the Industry Strategy Challenge Fund areas of "Accelerated detection of disease" and "From data to early diagnosis and precision medicine". The fellowship will enable exchange Dr Pentland's of biological skills, technical expertise, background knowledge and experience in using light sheet to image biofilms obtained within the FTMA award at Cairn Scientific with 3i scientists. Dr. Pentland will work with the 3i team, in consultation with Dr Laissue (Essex) to perfect image analysis of fungal biofilm dynamics using this remarkable technique. As Dr. Pentland will be trained on the Marianas Lightsheet system and software at 3i he will develop a deep understanding of industrial application, product design and market demands. He will receive support and technical advice throughout from applications scientist and Marianas™ Lightsheet product manager, Dr Hella Baumann. Dr. Baumann's time and access to 3i's facility are equivalent to an in-kind support in excess of £5,000 in value.
Collaborator Contribution The main output arises from the design, experimental testing and validation of light sheet microscopy methods in a new area of application: The developmental imaging of fungal biofilms are of medical, environmental and industrial relevance but is a research area that is currently unexplored. This fellowship will enable a step change in the imaging of fungal biofilms by coupling the fellows academic expertise with cutting edge advanced low-light fluorescence microscopy knowledge at 3i. An key output is therefore the transfer of knowledge between industry and academia to develop a new area of research, business opportunity and return to UK Plc. A key industrial output of this research will be to test and develop protocols for the Marianas Light Sheet system so that these can be distributed and used by multiple users, within a range of disciplines, to image fungal biofilms without inflicting photodamage. The fellow will work with 3i to develop protocols that can be rapidly adapted to different fungal biofilm applications, thus extending the applicability of this technology to research groups within medical, environmental and industrial fields. The research will allow development of integrated hardware and software control, creating a seamless user experience with comprehensive control of acquisition and rendering, ideal for a multi-user setting. The expected outputs are reliant on the exchange of skills and knowledge between the fellow and 3i scientists and will lead to tangible benefits to both parties.
Impact Di-SPIM light sheet microscopy is ideal for monitoring the development of a dense fungal biofilm. Our approaches will lead to this technology being applied to a new field and opens a new customer base 3i as was planned within this application.
Start Year 2021
 
Description NBIC FTMA Fellowship F_19_01 Impact of the potential interfering of Azospirillum brasilense Az39, one of the most used strains in agriculture inoculants in Argentina in polymicrobial biofilms and their interaction with the plant.(Miguel Camara) 
Organisation NOVA SA
Country Argentina 
Sector Private 
PI Contribution Studies in crop production require using novel strategies with low environmental impact. Some bacterial biofilms when associated with plant roots promote crop production (Plant Growth Promoting Rhizobacteria or PGPR) protecting them against environmental stress and diseases. Key for these interactions are small bacterial signal molecules which control the production of traits beneficial to the plant by a process known as quorum sensing (QS). Some bacterial plant pathogens also produce QS signals to trigger disease processes. Azospirillum brasilense Az39 is a key PGPR which can fix nitrogen for the plant and produce hormones which affect plant growth. It can degrade QS molecules and hence has potential to inactivate plant pathogens but also has the machinery to detect QS molecules although we still do not know how this affects the interaction with the plant. This fellowship will provide the required training to Dr. Gaston Lopez working at Rio Cuarto University (Argentina) in collaboration with the company NOVA SA to unravel how QS signal sensing and degradation 'impacts on the relationship between NOVA'S inoculant with Az39 (named Promozion), polymicrobial biofilms and the plant. This is paramount to understand the mechanisms behind the positive impact it has on crop production providing the stepping-stone required to improve natural inoculants using this bacterium for a wider use in agriculture in Argentina and other countries. The project will provide Dr. Gaston Lopez the know-how for the characterisation of QS inhibitory bacteria and the impact this has on plant growth and protection against diseases which he would take back to NOVA SA and Rio Cuarto University for the optimisation of inoculants for field trials. This project aligns with the Industrial Strategy Challenge Fund on Transforming Food Production through exploitation of natural inoculants and 101C Engineer theme. The long-term aims will be to improve agricultural productivity reducing the use of chemical treatments and hence their impact in the environment.
Collaborator Contribution Benefits to the Researcher: Dr. Gaston Lopez will gain know-how on the characterisation of quorum quenching bacteria including analytical chemistry techniques, construction of transcriptional fusions, tagging of bacteria for polymicrobial biofilms analysis, use of biofilms models and confocal microscopy, and determination of the specificity of QS receptors. Benefits to NBIC 1. Research in food security is at the heart of NOVA SA and Rio Cuarto University through their research expertise on bacterial-plant interactions and the optimisation of inoculants. NBIC will benefit through the delivery of sustainable solutions to global food challenges increasing the international research portfolio of this Centre. 2. It will lead to long-term collaborations between NBIC and the Argentinian applicants through applications to emerging calls from UKRI to enhance links with Argentina and the GCRF in the strategic area of sustainable agriculture. 3. Dr. Gaston Lopez will be giving seminars to NBIC researchers during his visit to Nottingham and explore future collaborations between NOVA SA the University of Rio Cuarto and NBIC. Benefits to the UK It will strengthen links with Argentina through access to the unique infrastructure for research on maize, wheat and soybean production, being one of the largest producers in the world. Especially as the PGPR studied in this project is a major inoculant for both crops used by NOVA SA. This project will contribute to the UK cross-government programme on Global Food Security research and the outcomes are likely to be translated into UK crops to reduce the use chemical fertilizers and pesticides. Benefits to Argentina The benefits to NOVA SA in particular and more generally to Argentina will be directly related to aiding the improvement of biofertilizers and biostimulants formulated with Azospirillum brasilense Az39 for the biological treatment of maize, wheat and soybean seeds or seedlings. The understanding of how the "cell to cell" communication mechanisms mediated by "quorum" signals modify the behaviour of this bacterium under agronomic conditions will become a "key tool" to improve the formulation of current commercial products with improved bacterial inoculant combinations, making them more efficient to promote plant growth under field conditions. The benefits will be reflected in the consolidation of an experimental line for this Argentinian team (NOVA SA and Rio Cuarto University), which yea entail an increase in the timber of specific joint publications with Nottingham University and the establishment of inks with NBIC for longer term collaborations.
Impact Although covid-19 reduced the time of my stay at Nottingham, I was able to make progress in the generation of some of the recombinant clones required to advance the understanding of the quorum sensing(QS)-quorum quenching (QS) system present in the plant growth promoting rhizobacteria (PGPR) A. brasilense Az39 with a view to study the impact of this system in crops inoculants. This was my trip outside Argentina and my stay at the Nottingham NBIC labs enabled me to acquire expertise in new molecular biology and analytical chemistry techniques. It also gave me the exposure to a very distinct way of working with opportunities to interact with scientists working in many disciplines but specially on biofilm research. This has also strengthened my CV and enabled me to apply for a permanent position as a CONICET researcher at the Universidad Nacional de Río Cuarto (application still under evaluation). Furthermore, the experience acquired during my stay allowed me to take on a part-time job as junior researcher in NOVA SA, the partner company who supported my FTMA application and work on the production of biofertilizers and in particular the strain I used in this project. NOVA SA is very interested in the project that I carried out during my stay at Nottingham. To enable me to continue with this project I have been given a PhD student, Sofía Nievas, who received a PhD scholarship from CONICET at the Universidad Nacional de Río Cuarto. This will ensure the continuation of the collaboration with Nottingham. Overall, my stay at an NBIC institution through this FTMA award, has allowed to lay the foundations to consolidate a longer-term international collaboration between Argentina (CONICET and UNRC) and the UK. We will continue to study how the QQ capacity of A. brasilense Az39 plays a role in the rhizosphere of crops and how this capacity can be exploited to develop new biofertilizers.
Start Year 2019
 
Description NBIC FTMA Fellowship F_19_01 Impact of the potential interfering of Azospirillum brasilense Az39, one of the most used strains in agriculture inoculants in Argentina in polymicrobial biofilms and their interaction with the plant.(Miguel Camara) 
Organisation National Biofilms Innovation Centre
Sector Private 
PI Contribution Studies in crop production require using novel strategies with low environmental impact. Some bacterial biofilms when associated with plant roots promote crop production (Plant Growth Promoting Rhizobacteria or PGPR) protecting them against environmental stress and diseases. Key for these interactions are small bacterial signal molecules which control the production of traits beneficial to the plant by a process known as quorum sensing (QS). Some bacterial plant pathogens also produce QS signals to trigger disease processes. Azospirillum brasilense Az39 is a key PGPR which can fix nitrogen for the plant and produce hormones which affect plant growth. It can degrade QS molecules and hence has potential to inactivate plant pathogens but also has the machinery to detect QS molecules although we still do not know how this affects the interaction with the plant. This fellowship will provide the required training to Dr. Gaston Lopez working at Rio Cuarto University (Argentina) in collaboration with the company NOVA SA to unravel how QS signal sensing and degradation 'impacts on the relationship between NOVA'S inoculant with Az39 (named Promozion), polymicrobial biofilms and the plant. This is paramount to understand the mechanisms behind the positive impact it has on crop production providing the stepping-stone required to improve natural inoculants using this bacterium for a wider use in agriculture in Argentina and other countries. The project will provide Dr. Gaston Lopez the know-how for the characterisation of QS inhibitory bacteria and the impact this has on plant growth and protection against diseases which he would take back to NOVA SA and Rio Cuarto University for the optimisation of inoculants for field trials. This project aligns with the Industrial Strategy Challenge Fund on Transforming Food Production through exploitation of natural inoculants and 101C Engineer theme. The long-term aims will be to improve agricultural productivity reducing the use of chemical treatments and hence their impact in the environment.
Collaborator Contribution Benefits to the Researcher: Dr. Gaston Lopez will gain know-how on the characterisation of quorum quenching bacteria including analytical chemistry techniques, construction of transcriptional fusions, tagging of bacteria for polymicrobial biofilms analysis, use of biofilms models and confocal microscopy, and determination of the specificity of QS receptors. Benefits to NBIC 1. Research in food security is at the heart of NOVA SA and Rio Cuarto University through their research expertise on bacterial-plant interactions and the optimisation of inoculants. NBIC will benefit through the delivery of sustainable solutions to global food challenges increasing the international research portfolio of this Centre. 2. It will lead to long-term collaborations between NBIC and the Argentinian applicants through applications to emerging calls from UKRI to enhance links with Argentina and the GCRF in the strategic area of sustainable agriculture. 3. Dr. Gaston Lopez will be giving seminars to NBIC researchers during his visit to Nottingham and explore future collaborations between NOVA SA the University of Rio Cuarto and NBIC. Benefits to the UK It will strengthen links with Argentina through access to the unique infrastructure for research on maize, wheat and soybean production, being one of the largest producers in the world. Especially as the PGPR studied in this project is a major inoculant for both crops used by NOVA SA. This project will contribute to the UK cross-government programme on Global Food Security research and the outcomes are likely to be translated into UK crops to reduce the use chemical fertilizers and pesticides. Benefits to Argentina The benefits to NOVA SA in particular and more generally to Argentina will be directly related to aiding the improvement of biofertilizers and biostimulants formulated with Azospirillum brasilense Az39 for the biological treatment of maize, wheat and soybean seeds or seedlings. The understanding of how the "cell to cell" communication mechanisms mediated by "quorum" signals modify the behaviour of this bacterium under agronomic conditions will become a "key tool" to improve the formulation of current commercial products with improved bacterial inoculant combinations, making them more efficient to promote plant growth under field conditions. The benefits will be reflected in the consolidation of an experimental line for this Argentinian team (NOVA SA and Rio Cuarto University), which yea entail an increase in the timber of specific joint publications with Nottingham University and the establishment of inks with NBIC for longer term collaborations.
Impact Although covid-19 reduced the time of my stay at Nottingham, I was able to make progress in the generation of some of the recombinant clones required to advance the understanding of the quorum sensing(QS)-quorum quenching (QS) system present in the plant growth promoting rhizobacteria (PGPR) A. brasilense Az39 with a view to study the impact of this system in crops inoculants. This was my trip outside Argentina and my stay at the Nottingham NBIC labs enabled me to acquire expertise in new molecular biology and analytical chemistry techniques. It also gave me the exposure to a very distinct way of working with opportunities to interact with scientists working in many disciplines but specially on biofilm research. This has also strengthened my CV and enabled me to apply for a permanent position as a CONICET researcher at the Universidad Nacional de Río Cuarto (application still under evaluation). Furthermore, the experience acquired during my stay allowed me to take on a part-time job as junior researcher in NOVA SA, the partner company who supported my FTMA application and work on the production of biofertilizers and in particular the strain I used in this project. NOVA SA is very interested in the project that I carried out during my stay at Nottingham. To enable me to continue with this project I have been given a PhD student, Sofía Nievas, who received a PhD scholarship from CONICET at the Universidad Nacional de Río Cuarto. This will ensure the continuation of the collaboration with Nottingham. Overall, my stay at an NBIC institution through this FTMA award, has allowed to lay the foundations to consolidate a longer-term international collaboration between Argentina (CONICET and UNRC) and the UK. We will continue to study how the QQ capacity of A. brasilense Az39 plays a role in the rhizosphere of crops and how this capacity can be exploited to develop new biofertilizers.
Start Year 2019
 
Description NBIC FTMA Fellowship F_19_01 Impact of the potential interfering of Azospirillum brasilense Az39, one of the most used strains in agriculture inoculants in Argentina in polymicrobial biofilms and their interaction with the plant.(Miguel Camara) 
Organisation University of Nottingham
Country United Kingdom 
Sector Academic/University 
PI Contribution Studies in crop production require using novel strategies with low environmental impact. Some bacterial biofilms when associated with plant roots promote crop production (Plant Growth Promoting Rhizobacteria or PGPR) protecting them against environmental stress and diseases. Key for these interactions are small bacterial signal molecules which control the production of traits beneficial to the plant by a process known as quorum sensing (QS). Some bacterial plant pathogens also produce QS signals to trigger disease processes. Azospirillum brasilense Az39 is a key PGPR which can fix nitrogen for the plant and produce hormones which affect plant growth. It can degrade QS molecules and hence has potential to inactivate plant pathogens but also has the machinery to detect QS molecules although we still do not know how this affects the interaction with the plant. This fellowship will provide the required training to Dr. Gaston Lopez working at Rio Cuarto University (Argentina) in collaboration with the company NOVA SA to unravel how QS signal sensing and degradation 'impacts on the relationship between NOVA'S inoculant with Az39 (named Promozion), polymicrobial biofilms and the plant. This is paramount to understand the mechanisms behind the positive impact it has on crop production providing the stepping-stone required to improve natural inoculants using this bacterium for a wider use in agriculture in Argentina and other countries. The project will provide Dr. Gaston Lopez the know-how for the characterisation of QS inhibitory bacteria and the impact this has on plant growth and protection against diseases which he would take back to NOVA SA and Rio Cuarto University for the optimisation of inoculants for field trials. This project aligns with the Industrial Strategy Challenge Fund on Transforming Food Production through exploitation of natural inoculants and 101C Engineer theme. The long-term aims will be to improve agricultural productivity reducing the use of chemical treatments and hence their impact in the environment.
Collaborator Contribution Benefits to the Researcher: Dr. Gaston Lopez will gain know-how on the characterisation of quorum quenching bacteria including analytical chemistry techniques, construction of transcriptional fusions, tagging of bacteria for polymicrobial biofilms analysis, use of biofilms models and confocal microscopy, and determination of the specificity of QS receptors. Benefits to NBIC 1. Research in food security is at the heart of NOVA SA and Rio Cuarto University through their research expertise on bacterial-plant interactions and the optimisation of inoculants. NBIC will benefit through the delivery of sustainable solutions to global food challenges increasing the international research portfolio of this Centre. 2. It will lead to long-term collaborations between NBIC and the Argentinian applicants through applications to emerging calls from UKRI to enhance links with Argentina and the GCRF in the strategic area of sustainable agriculture. 3. Dr. Gaston Lopez will be giving seminars to NBIC researchers during his visit to Nottingham and explore future collaborations between NOVA SA the University of Rio Cuarto and NBIC. Benefits to the UK It will strengthen links with Argentina through access to the unique infrastructure for research on maize, wheat and soybean production, being one of the largest producers in the world. Especially as the PGPR studied in this project is a major inoculant for both crops used by NOVA SA. This project will contribute to the UK cross-government programme on Global Food Security research and the outcomes are likely to be translated into UK crops to reduce the use chemical fertilizers and pesticides. Benefits to Argentina The benefits to NOVA SA in particular and more generally to Argentina will be directly related to aiding the improvement of biofertilizers and biostimulants formulated with Azospirillum brasilense Az39 for the biological treatment of maize, wheat and soybean seeds or seedlings. The understanding of how the "cell to cell" communication mechanisms mediated by "quorum" signals modify the behaviour of this bacterium under agronomic conditions will become a "key tool" to improve the formulation of current commercial products with improved bacterial inoculant combinations, making them more efficient to promote plant growth under field conditions. The benefits will be reflected in the consolidation of an experimental line for this Argentinian team (NOVA SA and Rio Cuarto University), which yea entail an increase in the timber of specific joint publications with Nottingham University and the establishment of inks with NBIC for longer term collaborations.
Impact Although covid-19 reduced the time of my stay at Nottingham, I was able to make progress in the generation of some of the recombinant clones required to advance the understanding of the quorum sensing(QS)-quorum quenching (QS) system present in the plant growth promoting rhizobacteria (PGPR) A. brasilense Az39 with a view to study the impact of this system in crops inoculants. This was my trip outside Argentina and my stay at the Nottingham NBIC labs enabled me to acquire expertise in new molecular biology and analytical chemistry techniques. It also gave me the exposure to a very distinct way of working with opportunities to interact with scientists working in many disciplines but specially on biofilm research. This has also strengthened my CV and enabled me to apply for a permanent position as a CONICET researcher at the Universidad Nacional de Río Cuarto (application still under evaluation). Furthermore, the experience acquired during my stay allowed me to take on a part-time job as junior researcher in NOVA SA, the partner company who supported my FTMA application and work on the production of biofertilizers and in particular the strain I used in this project. NOVA SA is very interested in the project that I carried out during my stay at Nottingham. To enable me to continue with this project I have been given a PhD student, Sofía Nievas, who received a PhD scholarship from CONICET at the Universidad Nacional de Río Cuarto. This will ensure the continuation of the collaboration with Nottingham. Overall, my stay at an NBIC institution through this FTMA award, has allowed to lay the foundations to consolidate a longer-term international collaboration between Argentina (CONICET and UNRC) and the UK. We will continue to study how the QQ capacity of A. brasilense Az39 plays a role in the rhizosphere of crops and how this capacity can be exploited to develop new biofertilizers.
Start Year 2019
 
Description NBIC FTMA Fellowship F_19_2_17 Organoids-on-a-chip for detecting and understanding biofilm formation in polymicrobial cystic fibrosis infections (José Juan-Colás) 
Organisation National Biofilms Innovation Centre
Sector Private 
PI Contribution Identifying and quantifying virulence, communication and antibiotic resistance in polymicrobial communities is crucial for understanding infection, improving diagnostics and for the development of targeted, personalised therapies. While current standardised Omics methods provide taxonomic specificity, they are destructive and lack spatiotemporal resolution for dynamically mapping early-biofilm formation in living bacterial communities. New technology is required that can non-destructively and rapidly quantify biofilm formation within the host-specific ecological conditions. This technology would enable testing of fundamental microbiological and clinically relevant questions, inform new diagnostic tools and enable screening of antimicrobial therapies, ultimately accelerating the detection of disease and developing leading-edge healthcare. By combining multi-modal spectroscopy (optical and electrochemical) and microfluidics, I am currently developing technology to spatiotemporally map early-biofilm formation in a system that mimics the ecological and environmental conditions of the Cystic Fibrosis(CF) lung. However, due to the absence of bacterial-host interactions, the phenotypic response of these CF-polymicrobial cultures differs from that observed in CF-patients. To fully understand communities in CF it is thus necessary to recreate the precise niche conditions, including interactions with host-cells. To achieve this, I will visit Prof. Molin in DTU(Denmark), who is an established international leader in the field of infectious chronic obstructive pulmonary disease. Prof. Molin is currently developing miniature organ models (organoids) in order to accurately mimic the natural environment of the CF-lung more closely than current 'test-tube' models of infection. This NBIC funding will allow me to engage with Prof. Molin and his team and to develop expertise in culturing epithelial stem cells into organoid models of human lung tissue. This expertise will subsequently be transferred to York to enhance on-going research in CF-biofilms. The know-how developed through this project would underpin follow-on research with foci in (a)novel tools to improve diagnosis and treatment of CF-bacterial infections and (b)understanding biofilm formation and bacteria-host interactions in CF-lungs.
Collaborator Contribution This project will provide a number of quantitative and qualitative benefits to my research, professional development and ultimately to different people at the University of York and the university itself. The successful realisation of this award will complement my expertise in engineering and physical sciences by allowing me to immerse myself in a clinically focussed research group specialising in experimental microbiology. This NBIC award will also help to position York at the forefront of clinical research and create new links between York and DTU through a long-lasting collaboration. The integration of organoid models of lung tissue with my novel analytical technologies will provide a new approach to study and understand CF in a realistic mimic of lung environments This will greatly enhance the clinical relevance of my current research on early-biofilm formation of model CF polymicrobial communities (which comprise Pseudomonas aureginosa, Staphylococcus aureus and Stenotrophomonas maltophilia), and allow my research to be published in high impact factor journals. Moreover, it would pave the way to generate the first publication, if not the first one of this kind that shows how realistic biofilm formation naturally occurs in a controlled system that reproduces the CF patients' microbiota and pathogen-host interactions. Upon completion of this award, the knowledge and skillset acquired to culture organoids, control the biochemical microenvironment of lung organoids, optimise the required nutrient supply and tailor the biophysical microenvironment would ultimately be transferred to York. Bringing this expertise to my current institution will directly enhance the activities of my local collaborators, Ville Friman and Michael Bottery, and expose them to new areas of research. The Friman and Bottery groups have already expressed an interest in employing the lung CF organoid in their research focussed on understanding how the CF focal pathogen (P. aeruginosa) evolves and adapts in the CF lung due to local microbiota and nutrient availability. This collaboration would be mutually beneficial, as my developed multi-modal sensing technology optimised for polymicrobial communities will complement the Molin group experimental capabilities. Specifically, it will allow his group to assess the mechanism and efficacy of novel targeted treatments of respiratory CF infections, in real-time and non-invasively. Thus, a visit to his lab would help establish a long-lasting collaboration and enable detailed and lengthy discussions with the group's members in order to support my upcoming Sir Henry Dale fellowship application with him as the principal collaborator, grounded in challenges in the field.
Impact We generated preliminary data and initially explored the viability of human enteroids to investigate early-biofilm formation in cystic fibrosis lung infections. We aim to use this data to (i) apply for a responsive mode grant with myself as the technical collaborator (lead by Soren Molin at DTU) and (ii) underpin a Sir Henry Dale fellowship application focused on developing a real-time, localised phenotyping technology based on a combination of Raman microscopy, machine learning and microfluidics. I also plan to further strengthen this preliminary data and carry out follow-up experiments in York to produce a joint publication that demonstrates how this technology that can spatiotemporally map pyocyanin regulation in realistic CF lung infections. Moreover, I engaged with individuals from a number of DTU health technology research groups to share expertise in developing different fluidic systems. This was very insightful to critically assess the viability of complementary microfluidic techniques (namely 3D printing and the in-house developed spin-disk approach) for studying biofilm formation in lung CF infections.
Start Year 2020
 
Description NBIC FTMA Fellowship F_19_2_17 Organoids-on-a-chip for detecting and understanding biofilm formation in polymicrobial cystic fibrosis infections (José Juan-Colás) 
Organisation Technical University of Denmark
Country Denmark 
Sector Academic/University 
PI Contribution Identifying and quantifying virulence, communication and antibiotic resistance in polymicrobial communities is crucial for understanding infection, improving diagnostics and for the development of targeted, personalised therapies. While current standardised Omics methods provide taxonomic specificity, they are destructive and lack spatiotemporal resolution for dynamically mapping early-biofilm formation in living bacterial communities. New technology is required that can non-destructively and rapidly quantify biofilm formation within the host-specific ecological conditions. This technology would enable testing of fundamental microbiological and clinically relevant questions, inform new diagnostic tools and enable screening of antimicrobial therapies, ultimately accelerating the detection of disease and developing leading-edge healthcare. By combining multi-modal spectroscopy (optical and electrochemical) and microfluidics, I am currently developing technology to spatiotemporally map early-biofilm formation in a system that mimics the ecological and environmental conditions of the Cystic Fibrosis(CF) lung. However, due to the absence of bacterial-host interactions, the phenotypic response of these CF-polymicrobial cultures differs from that observed in CF-patients. To fully understand communities in CF it is thus necessary to recreate the precise niche conditions, including interactions with host-cells. To achieve this, I will visit Prof. Molin in DTU(Denmark), who is an established international leader in the field of infectious chronic obstructive pulmonary disease. Prof. Molin is currently developing miniature organ models (organoids) in order to accurately mimic the natural environment of the CF-lung more closely than current 'test-tube' models of infection. This NBIC funding will allow me to engage with Prof. Molin and his team and to develop expertise in culturing epithelial stem cells into organoid models of human lung tissue. This expertise will subsequently be transferred to York to enhance on-going research in CF-biofilms. The know-how developed through this project would underpin follow-on research with foci in (a)novel tools to improve diagnosis and treatment of CF-bacterial infections and (b)understanding biofilm formation and bacteria-host interactions in CF-lungs.
Collaborator Contribution This project will provide a number of quantitative and qualitative benefits to my research, professional development and ultimately to different people at the University of York and the university itself. The successful realisation of this award will complement my expertise in engineering and physical sciences by allowing me to immerse myself in a clinically focussed research group specialising in experimental microbiology. This NBIC award will also help to position York at the forefront of clinical research and create new links between York and DTU through a long-lasting collaboration. The integration of organoid models of lung tissue with my novel analytical technologies will provide a new approach to study and understand CF in a realistic mimic of lung environments This will greatly enhance the clinical relevance of my current research on early-biofilm formation of model CF polymicrobial communities (which comprise Pseudomonas aureginosa, Staphylococcus aureus and Stenotrophomonas maltophilia), and allow my research to be published in high impact factor journals. Moreover, it would pave the way to generate the first publication, if not the first one of this kind that shows how realistic biofilm formation naturally occurs in a controlled system that reproduces the CF patients' microbiota and pathogen-host interactions. Upon completion of this award, the knowledge and skillset acquired to culture organoids, control the biochemical microenvironment of lung organoids, optimise the required nutrient supply and tailor the biophysical microenvironment would ultimately be transferred to York. Bringing this expertise to my current institution will directly enhance the activities of my local collaborators, Ville Friman and Michael Bottery, and expose them to new areas of research. The Friman and Bottery groups have already expressed an interest in employing the lung CF organoid in their research focussed on understanding how the CF focal pathogen (P. aeruginosa) evolves and adapts in the CF lung due to local microbiota and nutrient availability. This collaboration would be mutually beneficial, as my developed multi-modal sensing technology optimised for polymicrobial communities will complement the Molin group experimental capabilities. Specifically, it will allow his group to assess the mechanism and efficacy of novel targeted treatments of respiratory CF infections, in real-time and non-invasively. Thus, a visit to his lab would help establish a long-lasting collaboration and enable detailed and lengthy discussions with the group's members in order to support my upcoming Sir Henry Dale fellowship application with him as the principal collaborator, grounded in challenges in the field.
Impact We generated preliminary data and initially explored the viability of human enteroids to investigate early-biofilm formation in cystic fibrosis lung infections. We aim to use this data to (i) apply for a responsive mode grant with myself as the technical collaborator (lead by Soren Molin at DTU) and (ii) underpin a Sir Henry Dale fellowship application focused on developing a real-time, localised phenotyping technology based on a combination of Raman microscopy, machine learning and microfluidics. I also plan to further strengthen this preliminary data and carry out follow-up experiments in York to produce a joint publication that demonstrates how this technology that can spatiotemporally map pyocyanin regulation in realistic CF lung infections. Moreover, I engaged with individuals from a number of DTU health technology research groups to share expertise in developing different fluidic systems. This was very insightful to critically assess the viability of complementary microfluidic techniques (namely 3D printing and the in-house developed spin-disk approach) for studying biofilm formation in lung CF infections.
Start Year 2020
 
Description NBIC FTMA Fellowship F_19_2_17 Organoids-on-a-chip for detecting and understanding biofilm formation in polymicrobial cystic fibrosis infections (José Juan-Colás) 
Organisation University of York
Country United Kingdom 
Sector Academic/University 
PI Contribution Identifying and quantifying virulence, communication and antibiotic resistance in polymicrobial communities is crucial for understanding infection, improving diagnostics and for the development of targeted, personalised therapies. While current standardised Omics methods provide taxonomic specificity, they are destructive and lack spatiotemporal resolution for dynamically mapping early-biofilm formation in living bacterial communities. New technology is required that can non-destructively and rapidly quantify biofilm formation within the host-specific ecological conditions. This technology would enable testing of fundamental microbiological and clinically relevant questions, inform new diagnostic tools and enable screening of antimicrobial therapies, ultimately accelerating the detection of disease and developing leading-edge healthcare. By combining multi-modal spectroscopy (optical and electrochemical) and microfluidics, I am currently developing technology to spatiotemporally map early-biofilm formation in a system that mimics the ecological and environmental conditions of the Cystic Fibrosis(CF) lung. However, due to the absence of bacterial-host interactions, the phenotypic response of these CF-polymicrobial cultures differs from that observed in CF-patients. To fully understand communities in CF it is thus necessary to recreate the precise niche conditions, including interactions with host-cells. To achieve this, I will visit Prof. Molin in DTU(Denmark), who is an established international leader in the field of infectious chronic obstructive pulmonary disease. Prof. Molin is currently developing miniature organ models (organoids) in order to accurately mimic the natural environment of the CF-lung more closely than current 'test-tube' models of infection. This NBIC funding will allow me to engage with Prof. Molin and his team and to develop expertise in culturing epithelial stem cells into organoid models of human lung tissue. This expertise will subsequently be transferred to York to enhance on-going research in CF-biofilms. The know-how developed through this project would underpin follow-on research with foci in (a)novel tools to improve diagnosis and treatment of CF-bacterial infections and (b)understanding biofilm formation and bacteria-host interactions in CF-lungs.
Collaborator Contribution This project will provide a number of quantitative and qualitative benefits to my research, professional development and ultimately to different people at the University of York and the university itself. The successful realisation of this award will complement my expertise in engineering and physical sciences by allowing me to immerse myself in a clinically focussed research group specialising in experimental microbiology. This NBIC award will also help to position York at the forefront of clinical research and create new links between York and DTU through a long-lasting collaboration. The integration of organoid models of lung tissue with my novel analytical technologies will provide a new approach to study and understand CF in a realistic mimic of lung environments This will greatly enhance the clinical relevance of my current research on early-biofilm formation of model CF polymicrobial communities (which comprise Pseudomonas aureginosa, Staphylococcus aureus and Stenotrophomonas maltophilia), and allow my research to be published in high impact factor journals. Moreover, it would pave the way to generate the first publication, if not the first one of this kind that shows how realistic biofilm formation naturally occurs in a controlled system that reproduces the CF patients' microbiota and pathogen-host interactions. Upon completion of this award, the knowledge and skillset acquired to culture organoids, control the biochemical microenvironment of lung organoids, optimise the required nutrient supply and tailor the biophysical microenvironment would ultimately be transferred to York. Bringing this expertise to my current institution will directly enhance the activities of my local collaborators, Ville Friman and Michael Bottery, and expose them to new areas of research. The Friman and Bottery groups have already expressed an interest in employing the lung CF organoid in their research focussed on understanding how the CF focal pathogen (P. aeruginosa) evolves and adapts in the CF lung due to local microbiota and nutrient availability. This collaboration would be mutually beneficial, as my developed multi-modal sensing technology optimised for polymicrobial communities will complement the Molin group experimental capabilities. Specifically, it will allow his group to assess the mechanism and efficacy of novel targeted treatments of respiratory CF infections, in real-time and non-invasively. Thus, a visit to his lab would help establish a long-lasting collaboration and enable detailed and lengthy discussions with the group's members in order to support my upcoming Sir Henry Dale fellowship application with him as the principal collaborator, grounded in challenges in the field.
Impact We generated preliminary data and initially explored the viability of human enteroids to investigate early-biofilm formation in cystic fibrosis lung infections. We aim to use this data to (i) apply for a responsive mode grant with myself as the technical collaborator (lead by Soren Molin at DTU) and (ii) underpin a Sir Henry Dale fellowship application focused on developing a real-time, localised phenotyping technology based on a combination of Raman microscopy, machine learning and microfluidics. I also plan to further strengthen this preliminary data and carry out follow-up experiments in York to produce a joint publication that demonstrates how this technology that can spatiotemporally map pyocyanin regulation in realistic CF lung infections. Moreover, I engaged with individuals from a number of DTU health technology research groups to share expertise in developing different fluidic systems. This was very insightful to critically assess the viability of complementary microfluidic techniques (namely 3D printing and the in-house developed spin-disk approach) for studying biofilm formation in lung CF infections.
Start Year 2020
 
Description NBIC FTMA Fellowship F_19_2_31 Engineering cyanobacteria biofilms to transform production of aqua- and agri-culture feedstocks (Sarah Rouse) 
Organisation Cyanofeed Ltd
Country United Kingdom 
Sector Private 
PI Contribution The aim of the partnership is to optimise a new method for aqua- and agri-culture feedstock production, by using engineered biofilm-forming cyanobacteria as a food source. This 2 month industry-based project will bring together elements of academic research and industrial processes. This project is aligned to the UKRI Transforming Food Production Industry Challenge. Production of animal feed is ecologically damaging, and expensive. Fishmeal is usually derived from ocean prey fish and their stocks are being depleted. Using cyanobacteria and micro algae has been attempted in the past but farmed fish including salmon and trout are unable to digest large quantities because of the exopolysaccharides - important components of biofilm. Cyanofeed are engineering new strains to keep the beneficial aspects of biofilm whilst making them readily digestible to the fish. I will work with them to optimise these variants, using on site testing to determine microbial composition and the best methods to improve efficiency in harvesting and downstream processing. In the longer term, these same processes could potentially be used to make renewable protein sources for human consumption. Being awarded this NBIC Innovation Fellowship would pave the way for future translational research partnerships with industry. It will provide a foundation in the steps required for successful commercialisation of lab based work. I have a deep understanding of the underlying science of biofilm formation, but have limited experience of working in an industrial environment, in particular, the challenges associated with large scale production. Furthermore, being based at an early-stage biotech startup will involve exposure to more varied aspects of working within industry that I may not experience at a larger company.
Collaborator Contribution within a start-up will also allow me to be involved in company expansion and provides potential for long-term partnership. • Immediate potential collaboration: The 2 month secondment is expected to be the beginning of a longterm partnership. • Joint publications: Expect to publish a white paper on this method and present outcomes at research conferences. • Societal and economic impact: This fellowship will have both societal and economic impact. Finding new ways to make renewable protein sources in feedstock production is a high priority area of research. • Translational applications will arise from our understanding of how the engineered biofilm strains behave, and new insight into which properties of biofilm are beneficial for nutrition.
Impact We have designed a robust testing approach which will benefit the production of modified feedstocks. This is the first, measurable step within an important area of research which will have both societal and economic impact in the medium to long-term. Finding new ways to make renewable protein sources in feedstock production is a high priority area of research. We also expect to develop our testing protocols for translational applications that will arise from our understanding of how the engineered biofilm strains behave, and new insight into which properties of biofilm are beneficial for nutrition. This project is aligned to the UKRI Transforming Food Production Industry Challenge. The protocols designed during the FTMA Innovation Fellowship will be implemented in the next round of feeding trials. This Innovation Fellowship was intended to lay the groundwork for a longer-term partnership. The resources and support available from NBIC will be beneficial going forwards, and we hope to be able to attend any future training workshops.
Start Year 2020
 
Description NBIC FTMA Fellowship F_19_2_31 Engineering cyanobacteria biofilms to transform production of aqua- and agri-culture feedstocks (Sarah Rouse) 
Organisation Imperial College London
Country United Kingdom 
Sector Academic/University 
PI Contribution The aim of the partnership is to optimise a new method for aqua- and agri-culture feedstock production, by using engineered biofilm-forming cyanobacteria as a food source. This 2 month industry-based project will bring together elements of academic research and industrial processes. This project is aligned to the UKRI Transforming Food Production Industry Challenge. Production of animal feed is ecologically damaging, and expensive. Fishmeal is usually derived from ocean prey fish and their stocks are being depleted. Using cyanobacteria and micro algae has been attempted in the past but farmed fish including salmon and trout are unable to digest large quantities because of the exopolysaccharides - important components of biofilm. Cyanofeed are engineering new strains to keep the beneficial aspects of biofilm whilst making them readily digestible to the fish. I will work with them to optimise these variants, using on site testing to determine microbial composition and the best methods to improve efficiency in harvesting and downstream processing. In the longer term, these same processes could potentially be used to make renewable protein sources for human consumption. Being awarded this NBIC Innovation Fellowship would pave the way for future translational research partnerships with industry. It will provide a foundation in the steps required for successful commercialisation of lab based work. I have a deep understanding of the underlying science of biofilm formation, but have limited experience of working in an industrial environment, in particular, the challenges associated with large scale production. Furthermore, being based at an early-stage biotech startup will involve exposure to more varied aspects of working within industry that I may not experience at a larger company.
Collaborator Contribution within a start-up will also allow me to be involved in company expansion and provides potential for long-term partnership. • Immediate potential collaboration: The 2 month secondment is expected to be the beginning of a longterm partnership. • Joint publications: Expect to publish a white paper on this method and present outcomes at research conferences. • Societal and economic impact: This fellowship will have both societal and economic impact. Finding new ways to make renewable protein sources in feedstock production is a high priority area of research. • Translational applications will arise from our understanding of how the engineered biofilm strains behave, and new insight into which properties of biofilm are beneficial for nutrition.
Impact We have designed a robust testing approach which will benefit the production of modified feedstocks. This is the first, measurable step within an important area of research which will have both societal and economic impact in the medium to long-term. Finding new ways to make renewable protein sources in feedstock production is a high priority area of research. We also expect to develop our testing protocols for translational applications that will arise from our understanding of how the engineered biofilm strains behave, and new insight into which properties of biofilm are beneficial for nutrition. This project is aligned to the UKRI Transforming Food Production Industry Challenge. The protocols designed during the FTMA Innovation Fellowship will be implemented in the next round of feeding trials. This Innovation Fellowship was intended to lay the groundwork for a longer-term partnership. The resources and support available from NBIC will be beneficial going forwards, and we hope to be able to attend any future training workshops.
Start Year 2020
 
Description NBIC FTMA Fellowship F_19_2_31 Engineering cyanobacteria biofilms to transform production of aqua- and agri-culture feedstocks (Sarah Rouse) 
Organisation National Biofilms Innovation Centre
Sector Private 
PI Contribution The aim of the partnership is to optimise a new method for aqua- and agri-culture feedstock production, by using engineered biofilm-forming cyanobacteria as a food source. This 2 month industry-based project will bring together elements of academic research and industrial processes. This project is aligned to the UKRI Transforming Food Production Industry Challenge. Production of animal feed is ecologically damaging, and expensive. Fishmeal is usually derived from ocean prey fish and their stocks are being depleted. Using cyanobacteria and micro algae has been attempted in the past but farmed fish including salmon and trout are unable to digest large quantities because of the exopolysaccharides - important components of biofilm. Cyanofeed are engineering new strains to keep the beneficial aspects of biofilm whilst making them readily digestible to the fish. I will work with them to optimise these variants, using on site testing to determine microbial composition and the best methods to improve efficiency in harvesting and downstream processing. In the longer term, these same processes could potentially be used to make renewable protein sources for human consumption. Being awarded this NBIC Innovation Fellowship would pave the way for future translational research partnerships with industry. It will provide a foundation in the steps required for successful commercialisation of lab based work. I have a deep understanding of the underlying science of biofilm formation, but have limited experience of working in an industrial environment, in particular, the challenges associated with large scale production. Furthermore, being based at an early-stage biotech startup will involve exposure to more varied aspects of working within industry that I may not experience at a larger company.
Collaborator Contribution within a start-up will also allow me to be involved in company expansion and provides potential for long-term partnership. • Immediate potential collaboration: The 2 month secondment is expected to be the beginning of a longterm partnership. • Joint publications: Expect to publish a white paper on this method and present outcomes at research conferences. • Societal and economic impact: This fellowship will have both societal and economic impact. Finding new ways to make renewable protein sources in feedstock production is a high priority area of research. • Translational applications will arise from our understanding of how the engineered biofilm strains behave, and new insight into which properties of biofilm are beneficial for nutrition.
Impact We have designed a robust testing approach which will benefit the production of modified feedstocks. This is the first, measurable step within an important area of research which will have both societal and economic impact in the medium to long-term. Finding new ways to make renewable protein sources in feedstock production is a high priority area of research. We also expect to develop our testing protocols for translational applications that will arise from our understanding of how the engineered biofilm strains behave, and new insight into which properties of biofilm are beneficial for nutrition. This project is aligned to the UKRI Transforming Food Production Industry Challenge. The protocols designed during the FTMA Innovation Fellowship will be implemented in the next round of feeding trials. This Innovation Fellowship was intended to lay the groundwork for a longer-term partnership. The resources and support available from NBIC will be beneficial going forwards, and we hope to be able to attend any future training workshops.
Start Year 2020
 
Description NBIC FTMA Fellowship F_19_2_45 Superior Anti-biofilm Coatings for Urinary Catheters (Paolo Pantalone) 
Organisation National Biofilms Innovation Centre
Sector Private 
PI Contribution This project aims to provide novel insights regarding the interactions of bacterial urinary tract pathogens with anti-fouling polymers (Nottingham) combined with a novel anti-biofilm technology (lactams - Unilever). This creates a potential opportunity to develop superior catheter materials for preventing catheter associated urinary tract infections (CAUTI's). Various physical techniques will be employed to characterise the surfaces of lactam-treated materials using established techniques (supported by the Materials Innovation Factory). Single-cell tracking via multimode 2D-3D imaging and confocal microscopy will used to investigate bacteria-lactam surface interactions and multi-species biofilm formation in artificial urine. The project will facilitate development of my current knowledge surrounding lactam anti-biofilm by expanding to alternative mixed species biofilms. I will be using my intellectual abilities to translate into a previously unexplored area - combining fouling resistant polymers with the lactam material. This will require creative thought and problem solving on my part to deliver insights and areas for opportunities in an efficient manner. Freedom to manage the project will require working to deadlines within a multidisciplinary team. My professional network will be enhanced leading to future industrial collaborations. Alignment to ISC: Leading Edge Healthcare - Recognising the demands of an aging population and delivering superior technologies and products. Urinary tract catheters are the most commonly used prosthetic medical devices, with 15-25% of patients requiring bladder catheterisation during hospitalization. They are used to manage urine retention or incontinence for short and extended periods. Long-term catheter users with permanent disability will need a catheter change after 2-3 months for the rest of their lives. Catheters promote CAUTIs that, if left untreated, may lead to kidney infection, sepsis and death. Treatment requires antibiotic therapy and catheter removal. CAUTIs cost the NHS £99M pa and require an extra 638,000 extra bed days (House of Commons Select Committee Report).
Collaborator Contribution The primary deliverable would be demonstration of the superior efficacy of a novel catheter material that could be exploited and developed further in the future by both parties through ongoing collaboration. A key practical output from the project would be the application of multimode 2D-3D imaging to complex microbial systems. The work may also result in the generation of joint intellectual property and a publication output. Unilever will gain access to skills they require to develop their technology in an area outside of their current fields of use, for which they have recently created a spin-out company (Penrhos Bio Ltd). Output from this project will be fed back into Penrhos which could lead to advances in their business. This is an opportunity to deepen the ties they already have with University of Nottingham. Work from this project will further their aim to move towards a more environmentally friendly manufacturer of medical devices and other products. With funding for this placement, they gain the benefits of a skilled worker on their project who has the opportunity to dedicate the time and skills learned to a focused area of research. This project would also showcase Unilever's ability to work with NBIC with a wider scope. The University of Nottingham will deepen its ties with Unilever via a reciprocal relationship. This project could also lead to future student placements at Unilever. This is then further publicity that the University and NBIC are collectively providing further opportunities for students to realise their career ambitions. This project would also showcase Nottingham's active engagement in the government's Industrial Strategy as highlighted in the Universities' UK green paper of January 2017, and University Strategic Plan 2013-2018. NBIC Case Study: In early 2020 Dr Paolo Pantalone was an NBIC Associate Doctoral Researcher at the University of Nottingham and had been working in association with Unilever on elucidating the mechanism of action of novel agents (Unilever's Lactam Technology) for Pseudomonas aeruginosa biofilm prevention. The opportunity arose via the NBIC FTMA scheme to exploit potential novel applications of the Lactam compounds beyond the Unilever core sectors. Paolo worked on their use in combination with unique coatings for the prevention of catheter associated urinary tract infections developed at Nottingham. Also called CAUTI's, these cost the NHS £99m per year. According to a House of Commons Select Committee Report, these require an extra 638,000 extra bed days. Various physical techniques were employed at both Nottingham and in Unilever's Materials Innovation Factory based at Liverpool University. These aimed to characterise the anti-fouling properties of lactam-treated materials using established techniques such as Liquid Chromatography-Mass Spectrometry and confocal microscopy on a state-of-the-art biofilm model. Paolo said, "The project facilitated development of my current knowledge surrounding the lactam anti-biofilm technology and helped me to translate these agents into a previously unexplored area - applying the lactam material to commercial catheters. This required creative thought and problem solving to deliver insights and areas for opportunities in an efficient manner". Paolo's work also allowed his professional network to be enhanced and to work more closely with Unilever development teams and in their facilities. The project allowed Unilever to gain access to skills they required to develop their technology in an area outside of their current fields of use. Unilever recently created a spin-out company (Penrhos Bio Ltd) as a joint venture with the life sciences investment group Innova Partnerships to market the technology outside its normal market sectors. Paolo has now been successful in securing a job with Unilever, demonstrating not just his ability, but also the opportunities offered by collaborative programmes such as the FTMA for researchers to work alongside industry. Lactams, rather than killing bacteria, prevent micro-organisms from forming biofilms on surfaces by disrupting their communications systems. Through Proof of Concept funding, NBIC has funded other projects to allow Penrhos Bio Ltd to explore and prove, or disprove, other Lactam applications, for example marine antifouling with the Plymouth Marine Laboratory.
Impact The catheters treated with Unilever's compounds showed excellent catheter release and biofilm prevention properties on both CAUTI species tested during the project. The data generated by this study will unlock new applications for Unilever's lactam technology in the biomedical and industrial sectors. I will benefit by authorship of any future publications. In addition, Unilever have offered me a permanent position as a research scientist and so I will be able to continue the lactam work.
Start Year 2021
 
Description NBIC FTMA Fellowship F_19_2_45 Superior Anti-biofilm Coatings for Urinary Catheters (Paolo Pantalone) 
Organisation Unilever
Country United Kingdom 
Sector Private 
PI Contribution This project aims to provide novel insights regarding the interactions of bacterial urinary tract pathogens with anti-fouling polymers (Nottingham) combined with a novel anti-biofilm technology (lactams - Unilever). This creates a potential opportunity to develop superior catheter materials for preventing catheter associated urinary tract infections (CAUTI's). Various physical techniques will be employed to characterise the surfaces of lactam-treated materials using established techniques (supported by the Materials Innovation Factory). Single-cell tracking via multimode 2D-3D imaging and confocal microscopy will used to investigate bacteria-lactam surface interactions and multi-species biofilm formation in artificial urine. The project will facilitate development of my current knowledge surrounding lactam anti-biofilm by expanding to alternative mixed species biofilms. I will be using my intellectual abilities to translate into a previously unexplored area - combining fouling resistant polymers with the lactam material. This will require creative thought and problem solving on my part to deliver insights and areas for opportunities in an efficient manner. Freedom to manage the project will require working to deadlines within a multidisciplinary team. My professional network will be enhanced leading to future industrial collaborations. Alignment to ISC: Leading Edge Healthcare - Recognising the demands of an aging population and delivering superior technologies and products. Urinary tract catheters are the most commonly used prosthetic medical devices, with 15-25% of patients requiring bladder catheterisation during hospitalization. They are used to manage urine retention or incontinence for short and extended periods. Long-term catheter users with permanent disability will need a catheter change after 2-3 months for the rest of their lives. Catheters promote CAUTIs that, if left untreated, may lead to kidney infection, sepsis and death. Treatment requires antibiotic therapy and catheter removal. CAUTIs cost the NHS £99M pa and require an extra 638,000 extra bed days (House of Commons Select Committee Report).
Collaborator Contribution The primary deliverable would be demonstration of the superior efficacy of a novel catheter material that could be exploited and developed further in the future by both parties through ongoing collaboration. A key practical output from the project would be the application of multimode 2D-3D imaging to complex microbial systems. The work may also result in the generation of joint intellectual property and a publication output. Unilever will gain access to skills they require to develop their technology in an area outside of their current fields of use, for which they have recently created a spin-out company (Penrhos Bio Ltd). Output from this project will be fed back into Penrhos which could lead to advances in their business. This is an opportunity to deepen the ties they already have with University of Nottingham. Work from this project will further their aim to move towards a more environmentally friendly manufacturer of medical devices and other products. With funding for this placement, they gain the benefits of a skilled worker on their project who has the opportunity to dedicate the time and skills learned to a focused area of research. This project would also showcase Unilever's ability to work with NBIC with a wider scope. The University of Nottingham will deepen its ties with Unilever via a reciprocal relationship. This project could also lead to future student placements at Unilever. This is then further publicity that the University and NBIC are collectively providing further opportunities for students to realise their career ambitions. This project would also showcase Nottingham's active engagement in the government's Industrial Strategy as highlighted in the Universities' UK green paper of January 2017, and University Strategic Plan 2013-2018. NBIC Case Study: In early 2020 Dr Paolo Pantalone was an NBIC Associate Doctoral Researcher at the University of Nottingham and had been working in association with Unilever on elucidating the mechanism of action of novel agents (Unilever's Lactam Technology) for Pseudomonas aeruginosa biofilm prevention. The opportunity arose via the NBIC FTMA scheme to exploit potential novel applications of the Lactam compounds beyond the Unilever core sectors. Paolo worked on their use in combination with unique coatings for the prevention of catheter associated urinary tract infections developed at Nottingham. Also called CAUTI's, these cost the NHS £99m per year. According to a House of Commons Select Committee Report, these require an extra 638,000 extra bed days. Various physical techniques were employed at both Nottingham and in Unilever's Materials Innovation Factory based at Liverpool University. These aimed to characterise the anti-fouling properties of lactam-treated materials using established techniques such as Liquid Chromatography-Mass Spectrometry and confocal microscopy on a state-of-the-art biofilm model. Paolo said, "The project facilitated development of my current knowledge surrounding the lactam anti-biofilm technology and helped me to translate these agents into a previously unexplored area - applying the lactam material to commercial catheters. This required creative thought and problem solving to deliver insights and areas for opportunities in an efficient manner". Paolo's work also allowed his professional network to be enhanced and to work more closely with Unilever development teams and in their facilities. The project allowed Unilever to gain access to skills they required to develop their technology in an area outside of their current fields of use. Unilever recently created a spin-out company (Penrhos Bio Ltd) as a joint venture with the life sciences investment group Innova Partnerships to market the technology outside its normal market sectors. Paolo has now been successful in securing a job with Unilever, demonstrating not just his ability, but also the opportunities offered by collaborative programmes such as the FTMA for researchers to work alongside industry. Lactams, rather than killing bacteria, prevent micro-organisms from forming biofilms on surfaces by disrupting their communications systems. Through Proof of Concept funding, NBIC has funded other projects to allow Penrhos Bio Ltd to explore and prove, or disprove, other Lactam applications, for example marine antifouling with the Plymouth Marine Laboratory.
Impact The catheters treated with Unilever's compounds showed excellent catheter release and biofilm prevention properties on both CAUTI species tested during the project. The data generated by this study will unlock new applications for Unilever's lactam technology in the biomedical and industrial sectors. I will benefit by authorship of any future publications. In addition, Unilever have offered me a permanent position as a research scientist and so I will be able to continue the lactam work.
Start Year 2021
 
Description NBIC FTMA Fellowship F_19_2_45 Superior Anti-biofilm Coatings for Urinary Catheters (Paolo Pantalone) 
Organisation University of Nottingham
Country United Kingdom 
Sector Academic/University 
PI Contribution This project aims to provide novel insights regarding the interactions of bacterial urinary tract pathogens with anti-fouling polymers (Nottingham) combined with a novel anti-biofilm technology (lactams - Unilever). This creates a potential opportunity to develop superior catheter materials for preventing catheter associated urinary tract infections (CAUTI's). Various physical techniques will be employed to characterise the surfaces of lactam-treated materials using established techniques (supported by the Materials Innovation Factory). Single-cell tracking via multimode 2D-3D imaging and confocal microscopy will used to investigate bacteria-lactam surface interactions and multi-species biofilm formation in artificial urine. The project will facilitate development of my current knowledge surrounding lactam anti-biofilm by expanding to alternative mixed species biofilms. I will be using my intellectual abilities to translate into a previously unexplored area - combining fouling resistant polymers with the lactam material. This will require creative thought and problem solving on my part to deliver insights and areas for opportunities in an efficient manner. Freedom to manage the project will require working to deadlines within a multidisciplinary team. My professional network will be enhanced leading to future industrial collaborations. Alignment to ISC: Leading Edge Healthcare - Recognising the demands of an aging population and delivering superior technologies and products. Urinary tract catheters are the most commonly used prosthetic medical devices, with 15-25% of patients requiring bladder catheterisation during hospitalization. They are used to manage urine retention or incontinence for short and extended periods. Long-term catheter users with permanent disability will need a catheter change after 2-3 months for the rest of their lives. Catheters promote CAUTIs that, if left untreated, may lead to kidney infection, sepsis and death. Treatment requires antibiotic therapy and catheter removal. CAUTIs cost the NHS £99M pa and require an extra 638,000 extra bed days (House of Commons Select Committee Report).
Collaborator Contribution The primary deliverable would be demonstration of the superior efficacy of a novel catheter material that could be exploited and developed further in the future by both parties through ongoing collaboration. A key practical output from the project would be the application of multimode 2D-3D imaging to complex microbial systems. The work may also result in the generation of joint intellectual property and a publication output. Unilever will gain access to skills they require to develop their technology in an area outside of their current fields of use, for which they have recently created a spin-out company (Penrhos Bio Ltd). Output from this project will be fed back into Penrhos which could lead to advances in their business. This is an opportunity to deepen the ties they already have with University of Nottingham. Work from this project will further their aim to move towards a more environmentally friendly manufacturer of medical devices and other products. With funding for this placement, they gain the benefits of a skilled worker on their project who has the opportunity to dedicate the time and skills learned to a focused area of research. This project would also showcase Unilever's ability to work with NBIC with a wider scope. The University of Nottingham will deepen its ties with Unilever via a reciprocal relationship. This project could also lead to future student placements at Unilever. This is then further publicity that the University and NBIC are collectively providing further opportunities for students to realise their career ambitions. This project would also showcase Nottingham's active engagement in the government's Industrial Strategy as highlighted in the Universities' UK green paper of January 2017, and University Strategic Plan 2013-2018. NBIC Case Study: In early 2020 Dr Paolo Pantalone was an NBIC Associate Doctoral Researcher at the University of Nottingham and had been working in association with Unilever on elucidating the mechanism of action of novel agents (Unilever's Lactam Technology) for Pseudomonas aeruginosa biofilm prevention. The opportunity arose via the NBIC FTMA scheme to exploit potential novel applications of the Lactam compounds beyond the Unilever core sectors. Paolo worked on their use in combination with unique coatings for the prevention of catheter associated urinary tract infections developed at Nottingham. Also called CAUTI's, these cost the NHS £99m per year. According to a House of Commons Select Committee Report, these require an extra 638,000 extra bed days. Various physical techniques were employed at both Nottingham and in Unilever's Materials Innovation Factory based at Liverpool University. These aimed to characterise the anti-fouling properties of lactam-treated materials using established techniques such as Liquid Chromatography-Mass Spectrometry and confocal microscopy on a state-of-the-art biofilm model. Paolo said, "The project facilitated development of my current knowledge surrounding the lactam anti-biofilm technology and helped me to translate these agents into a previously unexplored area - applying the lactam material to commercial catheters. This required creative thought and problem solving to deliver insights and areas for opportunities in an efficient manner". Paolo's work also allowed his professional network to be enhanced and to work more closely with Unilever development teams and in their facilities. The project allowed Unilever to gain access to skills they required to develop their technology in an area outside of their current fields of use. Unilever recently created a spin-out company (Penrhos Bio Ltd) as a joint venture with the life sciences investment group Innova Partnerships to market the technology outside its normal market sectors. Paolo has now been successful in securing a job with Unilever, demonstrating not just his ability, but also the opportunities offered by collaborative programmes such as the FTMA for researchers to work alongside industry. Lactams, rather than killing bacteria, prevent micro-organisms from forming biofilms on surfaces by disrupting their communications systems. Through Proof of Concept funding, NBIC has funded other projects to allow Penrhos Bio Ltd to explore and prove, or disprove, other Lactam applications, for example marine antifouling with the Plymouth Marine Laboratory.
Impact The catheters treated with Unilever's compounds showed excellent catheter release and biofilm prevention properties on both CAUTI species tested during the project. The data generated by this study will unlock new applications for Unilever's lactam technology in the biomedical and industrial sectors. I will benefit by authorship of any future publications. In addition, Unilever have offered me a permanent position as a research scientist and so I will be able to continue the lactam work.
Start Year 2021
 
Description NBIC FTMA Fellowship F_19_2_49 Stimulating Host-Pathogen Interactions in Wound Biofilms using Poloxamer Surfactants (Mohamed El Mohtadi) 
Organisation 5D Health Protection Group Ltd
Country United Kingdom 
Sector Private 
PI Contribution The Innovation Fellowship will enable me to take a three month secondment with 5D Health Protection Group (5D HPG) Limited at the Centre of Excellence in Biofilm Science and Technologies in Liverpool. The secondment will pump-prime a new biofilm collaboration between academia and 5D HPG. Chronic wounds are common in the elderly and lead to substantial morbidity and mortality. Biofilm formation is a major problem in chronic wounds, resulting in chronic inflammation and delayed wound closure. The recalcitrance of biofilms to the host immune system and antimicrobial agents, together with the emergence of antimicrobial drug resistant bacteria, typically leads to poor resolution and increased risk of infection. The aim of this project is to combine the use of poloxamer surfactants with targeted stimulation of innate immune responses as a novel strategy to resolve wound biofilms and promote healing. This novel therapeutic approach to prevent/treat wound infections in the elderly whilst reducing the reliance on antibiotics aligns to two key challenges outlined in the Industry Strategic Challenge Fund, Healthy Ageing and Leading-Edge Healthcare. My hypothesis is that the prevention of biofilms and/or the dispersal of existing biofilms using poloxamer surfactants will provide an environment that promotes bacterial clearance through enhanced host inflammatory cell migration and interaction with bacteria. The poloxamer surfactants will be investigated alone and in combination with immunomodulatory agents investigated during my PhD (awarded 2019) that dampen inflammation but promote phagocytosis and healing. As a non-British, early career postdoctoral research scientist with expertise in host-pathogen interactions, this opportunity to work in biofilm research and gain experience of an industrial research environment will grow my skills in the field. Successful completion of the fellowship will enhance my research profile and attractiveness to UK employers/funders, thereby supporting my career plans to remain in the UK and eventually become an independent researcher.
Collaborator Contribution This secondment will extend my expertise of host-pathogen interactions into the field of biofilm management and control. It will give me an insight in industry developments and technologies, and provide me with industry perspective that will enhance my career aspirations and prospects. The Innovation Fellowship will pump-prime an exciting new biofilm collaboration between Manchester Metropolitan University and the 5D HPG industry partner. The project will generate sufficient data to substantially contribute to one subsequent joint research publication (submission to Biofilms and Microbiomes proposed) and kick-start larger funding bids (BBSRC Industrial Partnership Award/Responsive Mode Research Grant under consideration) to sustain an ongoing income stream and build the research capacity as the collaboration develops beyond this current application. This initial collaborative project will focus predominantly on stimulation of macrophage-mediated host inflammatory cell activity but future work will explore a wider range of host-pathogen interactions and wound healing processes. The 5D HPG industry partner already has patent-protected antibiofilm technologies in relation to its surfactant agents and this collaboration with academia is intended to lead to a long-lasting partnership where further, potential game-changing therapeutic interventions with commercial exploitability become realised. The use of poloxamer surfactants alongside stimulation of host immunity represents an emergent strategy to combat biofilms in impaired wound healing states, leading to far-reaching societal, economic and healthcare impact. The NHS spends over £5 billion per year treating chronic wounds and associated comorbidities. However, wound treatments often fail and have been largely limited to debridement of non-viable (necrotic) tissues, application of routine wound dressings and use of antibiotics to clear wound infections. Biofilm formation can impede antibiotic delivery and impair bacterial clearance by host immunity. Moreover, antibiotic resistance is commonplace so there is an urgent need to develop new therapeutic strategies to resolve infections with reduced reliance on antibiotics. This project will foster a close partnership to develop and optimise novel, commercially viable therapies in the future that simultaneously prevent/control biofilm formation and promote host-mediated clearance of bacteria, with impacts that include • Reduced antibiotic use [thereby helping to tackle antibiotic resistance and emergence of new resistant strains]. • Prophylactic use to prevent wound infections in patients with ulcer development. • Enhanced healing and reduced wound infection rates in the elderly. • Reduced healthcare costs and treatment times. • Improved health, longevity and wellbeing in the elderly. • Reduced morbidity (e.g. impaired mobility, pain and anxiety/depression) and issues of social isolation/exclusion associated with chronic wounds.
Impact This short (3-month) fellowship project has generated a substantial amount of high quality, novel data that can be used to write a research manuscript for publication and form the basis for further funding opportunities and a growing collaboration between researchers at Manchester Metropolitan University and 5D Health Protection Group (5D HPG) Limited. The award enabled the named fellow (Mohamed El Mohtadi) to work in close partnership with 5D HPG Limited, gaining invaluable expertise in biofilm investigations at their laboratories, experience of working within an external company setting and fostering a strong collaborative link between academia and industry. This opportunity has also contributed to the fellow subsequently securing a permanent academic post in the UK with the ability to set up a new research group in this particular field. The findings of this study confirmed that dual sequential use of poloxamers followed by estradiol therapy substantially enhances both the disruption of Gram-positive or Gram-negative bacterial biofilms in vitro and their subsequent targeted clearance by host inflammatory cells when compared to either poloxamer or estradiol application alone. Moreover, this combined therapeutic strategy not only promoted biofilm disruption and host-mediated clearance without the need for antibiotics but also stimulated rapid wound repair, thereby providing multiple beneficial advantages that could be exploited in clinical settings such as infected chronic wounds in the elderly. These initial findings using in vitro biofilm and wound models are extremely promising but future work needs to determine whether the results can be extrapolated into in vivo wound settings. Successful future development of topical wound therapies using this focussed strategy and reduced reliance on antibiotics could revolutionise wound healing and anti-biofilm technologies, leading to substantial clinical and economic impact for the management and treatment of chronic wounds in the elderly. The potential development of game-changing therapies arising from this project may lead to far-reaching societal, economic and healthcare impact.
Start Year 2020
 
Description NBIC FTMA Fellowship F_19_2_49 Stimulating Host-Pathogen Interactions in Wound Biofilms using Poloxamer Surfactants (Mohamed El Mohtadi) 
Organisation Manchester Metropolitan University
Country United Kingdom 
Sector Academic/University 
PI Contribution The Innovation Fellowship will enable me to take a three month secondment with 5D Health Protection Group (5D HPG) Limited at the Centre of Excellence in Biofilm Science and Technologies in Liverpool. The secondment will pump-prime a new biofilm collaboration between academia and 5D HPG. Chronic wounds are common in the elderly and lead to substantial morbidity and mortality. Biofilm formation is a major problem in chronic wounds, resulting in chronic inflammation and delayed wound closure. The recalcitrance of biofilms to the host immune system and antimicrobial agents, together with the emergence of antimicrobial drug resistant bacteria, typically leads to poor resolution and increased risk of infection. The aim of this project is to combine the use of poloxamer surfactants with targeted stimulation of innate immune responses as a novel strategy to resolve wound biofilms and promote healing. This novel therapeutic approach to prevent/treat wound infections in the elderly whilst reducing the reliance on antibiotics aligns to two key challenges outlined in the Industry Strategic Challenge Fund, Healthy Ageing and Leading-Edge Healthcare. My hypothesis is that the prevention of biofilms and/or the dispersal of existing biofilms using poloxamer surfactants will provide an environment that promotes bacterial clearance through enhanced host inflammatory cell migration and interaction with bacteria. The poloxamer surfactants will be investigated alone and in combination with immunomodulatory agents investigated during my PhD (awarded 2019) that dampen inflammation but promote phagocytosis and healing. As a non-British, early career postdoctoral research scientist with expertise in host-pathogen interactions, this opportunity to work in biofilm research and gain experience of an industrial research environment will grow my skills in the field. Successful completion of the fellowship will enhance my research profile and attractiveness to UK employers/funders, thereby supporting my career plans to remain in the UK and eventually become an independent researcher.
Collaborator Contribution This secondment will extend my expertise of host-pathogen interactions into the field of biofilm management and control. It will give me an insight in industry developments and technologies, and provide me with industry perspective that will enhance my career aspirations and prospects. The Innovation Fellowship will pump-prime an exciting new biofilm collaboration between Manchester Metropolitan University and the 5D HPG industry partner. The project will generate sufficient data to substantially contribute to one subsequent joint research publication (submission to Biofilms and Microbiomes proposed) and kick-start larger funding bids (BBSRC Industrial Partnership Award/Responsive Mode Research Grant under consideration) to sustain an ongoing income stream and build the research capacity as the collaboration develops beyond this current application. This initial collaborative project will focus predominantly on stimulation of macrophage-mediated host inflammatory cell activity but future work will explore a wider range of host-pathogen interactions and wound healing processes. The 5D HPG industry partner already has patent-protected antibiofilm technologies in relation to its surfactant agents and this collaboration with academia is intended to lead to a long-lasting partnership where further, potential game-changing therapeutic interventions with commercial exploitability become realised. The use of poloxamer surfactants alongside stimulation of host immunity represents an emergent strategy to combat biofilms in impaired wound healing states, leading to far-reaching societal, economic and healthcare impact. The NHS spends over £5 billion per year treating chronic wounds and associated comorbidities. However, wound treatments often fail and have been largely limited to debridement of non-viable (necrotic) tissues, application of routine wound dressings and use of antibiotics to clear wound infections. Biofilm formation can impede antibiotic delivery and impair bacterial clearance by host immunity. Moreover, antibiotic resistance is commonplace so there is an urgent need to develop new therapeutic strategies to resolve infections with reduced reliance on antibiotics. This project will foster a close partnership to develop and optimise novel, commercially viable therapies in the future that simultaneously prevent/control biofilm formation and promote host-mediated clearance of bacteria, with impacts that include • Reduced antibiotic use [thereby helping to tackle antibiotic resistance and emergence of new resistant strains]. • Prophylactic use to prevent wound infections in patients with ulcer development. • Enhanced healing and reduced wound infection rates in the elderly. • Reduced healthcare costs and treatment times. • Improved health, longevity and wellbeing in the elderly. • Reduced morbidity (e.g. impaired mobility, pain and anxiety/depression) and issues of social isolation/exclusion associated with chronic wounds.
Impact This short (3-month) fellowship project has generated a substantial amount of high quality, novel data that can be used to write a research manuscript for publication and form the basis for further funding opportunities and a growing collaboration between researchers at Manchester Metropolitan University and 5D Health Protection Group (5D HPG) Limited. The award enabled the named fellow (Mohamed El Mohtadi) to work in close partnership with 5D HPG Limited, gaining invaluable expertise in biofilm investigations at their laboratories, experience of working within an external company setting and fostering a strong collaborative link between academia and industry. This opportunity has also contributed to the fellow subsequently securing a permanent academic post in the UK with the ability to set up a new research group in this particular field. The findings of this study confirmed that dual sequential use of poloxamers followed by estradiol therapy substantially enhances both the disruption of Gram-positive or Gram-negative bacterial biofilms in vitro and their subsequent targeted clearance by host inflammatory cells when compared to either poloxamer or estradiol application alone. Moreover, this combined therapeutic strategy not only promoted biofilm disruption and host-mediated clearance without the need for antibiotics but also stimulated rapid wound repair, thereby providing multiple beneficial advantages that could be exploited in clinical settings such as infected chronic wounds in the elderly. These initial findings using in vitro biofilm and wound models are extremely promising but future work needs to determine whether the results can be extrapolated into in vivo wound settings. Successful future development of topical wound therapies using this focussed strategy and reduced reliance on antibiotics could revolutionise wound healing and anti-biofilm technologies, leading to substantial clinical and economic impact for the management and treatment of chronic wounds in the elderly. The potential development of game-changing therapies arising from this project may lead to far-reaching societal, economic and healthcare impact.
Start Year 2020
 
Description NBIC FTMA Fellowship F_19_2_49 Stimulating Host-Pathogen Interactions in Wound Biofilms using Poloxamer Surfactants (Mohamed El Mohtadi) 
Organisation National Biofilms Innovation Centre
Sector Private 
PI Contribution The Innovation Fellowship will enable me to take a three month secondment with 5D Health Protection Group (5D HPG) Limited at the Centre of Excellence in Biofilm Science and Technologies in Liverpool. The secondment will pump-prime a new biofilm collaboration between academia and 5D HPG. Chronic wounds are common in the elderly and lead to substantial morbidity and mortality. Biofilm formation is a major problem in chronic wounds, resulting in chronic inflammation and delayed wound closure. The recalcitrance of biofilms to the host immune system and antimicrobial agents, together with the emergence of antimicrobial drug resistant bacteria, typically leads to poor resolution and increased risk of infection. The aim of this project is to combine the use of poloxamer surfactants with targeted stimulation of innate immune responses as a novel strategy to resolve wound biofilms and promote healing. This novel therapeutic approach to prevent/treat wound infections in the elderly whilst reducing the reliance on antibiotics aligns to two key challenges outlined in the Industry Strategic Challenge Fund, Healthy Ageing and Leading-Edge Healthcare. My hypothesis is that the prevention of biofilms and/or the dispersal of existing biofilms using poloxamer surfactants will provide an environment that promotes bacterial clearance through enhanced host inflammatory cell migration and interaction with bacteria. The poloxamer surfactants will be investigated alone and in combination with immunomodulatory agents investigated during my PhD (awarded 2019) that dampen inflammation but promote phagocytosis and healing. As a non-British, early career postdoctoral research scientist with expertise in host-pathogen interactions, this opportunity to work in biofilm research and gain experience of an industrial research environment will grow my skills in the field. Successful completion of the fellowship will enhance my research profile and attractiveness to UK employers/funders, thereby supporting my career plans to remain in the UK and eventually become an independent researcher.
Collaborator Contribution This secondment will extend my expertise of host-pathogen interactions into the field of biofilm management and control. It will give me an insight in industry developments and technologies, and provide me with industry perspective that will enhance my career aspirations and prospects. The Innovation Fellowship will pump-prime an exciting new biofilm collaboration between Manchester Metropolitan University and the 5D HPG industry partner. The project will generate sufficient data to substantially contribute to one subsequent joint research publication (submission to Biofilms and Microbiomes proposed) and kick-start larger funding bids (BBSRC Industrial Partnership Award/Responsive Mode Research Grant under consideration) to sustain an ongoing income stream and build the research capacity as the collaboration develops beyond this current application. This initial collaborative project will focus predominantly on stimulation of macrophage-mediated host inflammatory cell activity but future work will explore a wider range of host-pathogen interactions and wound healing processes. The 5D HPG industry partner already has patent-protected antibiofilm technologies in relation to its surfactant agents and this collaboration with academia is intended to lead to a long-lasting partnership where further, potential game-changing therapeutic interventions with commercial exploitability become realised. The use of poloxamer surfactants alongside stimulation of host immunity represents an emergent strategy to combat biofilms in impaired wound healing states, leading to far-reaching societal, economic and healthcare impact. The NHS spends over £5 billion per year treating chronic wounds and associated comorbidities. However, wound treatments often fail and have been largely limited to debridement of non-viable (necrotic) tissues, application of routine wound dressings and use of antibiotics to clear wound infections. Biofilm formation can impede antibiotic delivery and impair bacterial clearance by host immunity. Moreover, antibiotic resistance is commonplace so there is an urgent need to develop new therapeutic strategies to resolve infections with reduced reliance on antibiotics. This project will foster a close partnership to develop and optimise novel, commercially viable therapies in the future that simultaneously prevent/control biofilm formation and promote host-mediated clearance of bacteria, with impacts that include • Reduced antibiotic use [thereby helping to tackle antibiotic resistance and emergence of new resistant strains]. • Prophylactic use to prevent wound infections in patients with ulcer development. • Enhanced healing and reduced wound infection rates in the elderly. • Reduced healthcare costs and treatment times. • Improved health, longevity and wellbeing in the elderly. • Reduced morbidity (e.g. impaired mobility, pain and anxiety/depression) and issues of social isolation/exclusion associated with chronic wounds.
Impact This short (3-month) fellowship project has generated a substantial amount of high quality, novel data that can be used to write a research manuscript for publication and form the basis for further funding opportunities and a growing collaboration between researchers at Manchester Metropolitan University and 5D Health Protection Group (5D HPG) Limited. The award enabled the named fellow (Mohamed El Mohtadi) to work in close partnership with 5D HPG Limited, gaining invaluable expertise in biofilm investigations at their laboratories, experience of working within an external company setting and fostering a strong collaborative link between academia and industry. This opportunity has also contributed to the fellow subsequently securing a permanent academic post in the UK with the ability to set up a new research group in this particular field. The findings of this study confirmed that dual sequential use of poloxamers followed by estradiol therapy substantially enhances both the disruption of Gram-positive or Gram-negative bacterial biofilms in vitro and their subsequent targeted clearance by host inflammatory cells when compared to either poloxamer or estradiol application alone. Moreover, this combined therapeutic strategy not only promoted biofilm disruption and host-mediated clearance without the need for antibiotics but also stimulated rapid wound repair, thereby providing multiple beneficial advantages that could be exploited in clinical settings such as infected chronic wounds in the elderly. These initial findings using in vitro biofilm and wound models are extremely promising but future work needs to determine whether the results can be extrapolated into in vivo wound settings. Successful future development of topical wound therapies using this focussed strategy and reduced reliance on antibiotics could revolutionise wound healing and anti-biofilm technologies, leading to substantial clinical and economic impact for the management and treatment of chronic wounds in the elderly. The potential development of game-changing therapies arising from this project may lead to far-reaching societal, economic and healthcare impact.
Start Year 2020
 
Description NBIC FTMA Placement 20_IP_085 Novel rapid methods for broad detection of microbes and microbial activity (Praveen Kaveri) 
Organisation Cromerix Ltd
Country United Kingdom 
Sector Private 
PI Contribution My PhD research experience at Loughborough University showed that while it is possible to detect bacteria in principle in a single step using an aptamer probe, there are several challenges to address before this can be successfully applied in practice. In this project, I aim to explore some novel approaches that can address the fundamental challenges around rapid aptamer probe-based detection and make the approach reliable and broadly applicable against any microbe (WP1 and WP2). I will also explore a novel approach for the detection of microbial activity (WP3). The experience of working in Cromerix, a start-up, will be the first of its kind for me as I have never worked in a commercial environment. Unique personal development will be achieved through interactions with client organisations, who bring in specific requirements around the detection of microbes, biofilms and microbial activity. Initial interactions with them have helped in shaping up the problem definition and inspired the novel methods of addressing those problems that I will explore through this project. I also look forward to gaining an exposure of the Alderley Park facilities where Cromerix has been offered a hotdesk. Success of this project will establish the proof of concept of novel rapid methods for broad detection of microbes and microbial activity for the first time. These methods have wide applicability in industry, clinic and home. Thus, this project aligns well with three of the four themes of the Industrial Strategy Challenge Fund: Ageing Society (Accelerating detection of disease, Leading-edge healthcare), Future of mobility (Future flight) and Clean Growth (Transforming food production).
Collaborator Contribution The following outcomes are expected as a result of this visit/placement. 1. New Skills and Knowledge - I will develop new knowledge around the feasibility of some novel generic methods for rapid broad detection of microbes and microbial activity using a. single aptamer probe (WP1), b. dual aptamer probe (WP1), c. catalytic transduction (WP2), and d. enzymatic cleavage (WP3). - I will learn how to liaise, communicate, present and submit reports to industrial clients. - As I aim to progress to the next phase of development working on the successful outcomes of this project, I will gain experience of writing collaborative proposals to funders such as Innovate-UK by working together with the clients. - As an early member of the start-up, I will gain exposure to the key small business operations, including purchase ordering, safety, and intellectual property management. 2. Collaborations This project proposal is guided by the "problem definition" discussions (scoping meetings) Cromerix has had with two established global players in the field of air transport (for microbial biofilms detection) and personal care (for microbial activity detection), where I have participated. The work I will carry out in this project will strengthen these collaborations and potentially lead to investments from these organisations for further technology development, an interest for which has been expressed by these organisations. Successful validation of the proposed generic methods will also allow me to approach organisations in other sectors of industry and clinic to develop new collaborations. 3. Impact Successful validation in this project will lead to further technology development to progress along the TRL and produce workable prototypes in collaboration with the industry partners. The methods being explored have broad applications. The chosen applications are based on interests received from industry players in niche sectors and can be extended to broader sectors of industry, clinic and consumer market. Thus, successful validation and TRL progression based on these methods can be potentially deliver a broad socio-economic impact through new product development around the detection of microbes and microbial activity. I look forward to working with the Alderley Park network as part of Cromerix in creating these translational impacts.
Impact Feedback from academic: I have met and exceeded my objectives of the FTMA project through the following outcomes. Skills and Knowledge The FTMA project gave me a unique experience of working in a start-up environment, which I found different from the academic research experience in many ways. The projects here are more industrial problem-driven rather than curiosity-driven as in a university. There is a greater importance on freedom to operate check before working on an idea. I helped the company Director with background IP search for discussion with the IP attorney, which helped ascertain the freedom to operate of the research I conducted in FTMA. My attending of the client meetings with the Director helped me understand how professionally these meetings are conducted, how NDAs are prepared and signed prior to the first meeting, how presentations are done, and how a problem is defined that is of importance to the client and deliverable within the required timeline, costs, and capacities of a start-up company. Working with the Director, I also learnt how to write industry-focused technical consulting and development proposals, two of which were submitted to clients, and one was submitted to Innovate UK. I have also gained some insights into the importance and challenges of contract negotiations before a project can actually start. The company was supported by NBIC's Accelerator funding for participating in the Alderley Park Accelerator. The Director primarily attended this, but I had the opportunity of getting introduced to the concepts of customer discovery, business plan proposition, and Lean innovation as these are now part of the company's knowledge base and have defined how we as a company approach business development. As an early member of the start-up, I have also gained exposure to the purchase and sales processes and setting up safety and risk assessment documents. Research outcomes For bacterial detection, the key outcomes included • Conserved epitopes for bacteria and novel aptamer beacon probes for broad detection of gram-negative bacteria that are applicable in various fields, including but not restricted to bacterial contamination in industry and environment, and clinical diagnostics of bacterial infections. • A novel method to detect viable bacteria using dual aptamer probes that is applicable for viability detection of other microbes, including fungi. • Conserved proteases and peptide probe for detection of bacterial activity. • A novel colorimetric transduction method for reader-less visual detection using peptide and aptamer probes. This method can be extended to detection of targets beyond bacteria. For fungal detection, the key outcomes included • Conserved epitopes and aptamers against these conserved epitopes with information on regions of interactions, which is now being used to design aptamer beacons for broad fungal detection as part of a client-funded project. For malodour detection and inhibition, the key outcomes included • Identifying the mechanism of malodour production by bacteria secreted from the human axillary glands. • Methods of detection of PatB, an enzyme associated with malodour production, detection of PatB activity and detection of malodour molecules produced by the bacteria. • A method and aptamer for inhibition of production of malodour molecules. Collaborations The outcomes of this FTMA project supported the progression of discussions with two clients, one in a niche field of microbial detection and another in personal care/microbiome. This involved submission of proposals to these organisations. The proposal to the first client (microbial detection) has been accepted after several rounds of discussion and has progressed towards contract negotiation phase. The project is scheduled to commence in August 2022 and will employ me. Progression with the proposal submitted to the second client (personal care/microbiome) has been put on hold by them, reportedly due to their organisational changes. Even if the second client is unable to progress, the outcome should help Cromerix to progress a collaboration on this topic with another organisation in the field of personal care/microbiome. In addition to the abovementioned collaboration, the outcomes of the project have also helped the company to engage with a wider network of stakeholders in the field of microbial detection, which is expected to drive more innovation-led projects supporting technology and business development. Impact The work done in the FTMA project is creating impact through multiple channels. The research outcomes have helped establish a client project in a niche field of microbial contamination detection, which is going to commence from August 2022, and has set strong foundations for a project in the field of personal care/microbiome. The outcomes also formed the basis of a recent application to the Fast Start Innovate-UK grant in the field of diabetic foot ulcer prognostic. All these projects have technology and business plan development in scope to support the eventual commercial success of the company. The outcomes are also supporting the growth in network and visibility of the company among the relevant stakeholders. Eventual adoption of the developed microbial detection technologies by the industry or clinic will address multiple themes of the Industrial Strategy Challenge Fund, some of these being Ageing Society (Accelerating detection of disease, Leading-edge healthcare), Future of mobility (Future flight) and Clean Growth (Transforming food production). With an outstanding support from the Director, the project has helped my personal development through the first and rich exposure to working in a business environment. The varied professional skills and knowledge gained around business case preparation, product development, IP, legal, safety and operations will help me contribute much better than before in my role as a "Scientist - Technology Development" in Cromerix. INTELLECTUAL PROPERTY In the near future, I will work closely with the IP attorney on patent filing around the method and materials developed within the FTMA project after doing a patentability assessment and IP strategy development together with the Director. WHAT DID HAPPEN NEXT? OR IS GOING TO HAPPEN NEXT? As mentioned above, the immediate next step is to work on a client project for microbial detection technology development in a niche space. Through this project, I will keep working with the team Cromerix on microbial detection technology development and progression towards commercialisation. Any support through direct funding from NBIC in a future proof of concept (POC) project or cash/in-kind support towards a funding application to another organisation for advancing the TRL of the technology will be vitally important and gratefully received. This FTMA funding was a unique support towards my gaining exposure in a technology start-up environment, which as an innovator I was aiming to get straight after my PhD. In addition to that, I am also getting a position in the company as a full-time employee. The funding also helped the company generate its own IP, which is acting as a foundation to its first projects and proposals. However, further data need to be generated before these IP can be filed as a patent. I hope we can apply for funding support from NBIC for patent filing at the time when it is needed. Also, any support towards my continued professional development (CPD), either through courses within NBIC or funding support to attend these externally will be very helpful and much appreciated. I would like to extend my sincerest thanks to the FTMA funding, which has helped unleash the potential in me and the company to take the first steps towards creating valuable impact to UK's society, economy, and environment.
Start Year 2021
 
Description NBIC FTMA Placement 20_IP_085 Novel rapid methods for broad detection of microbes and microbial activity (Praveen Kaveri) 
Organisation Loughborough University
Country United Kingdom 
Sector Academic/University 
PI Contribution My PhD research experience at Loughborough University showed that while it is possible to detect bacteria in principle in a single step using an aptamer probe, there are several challenges to address before this can be successfully applied in practice. In this project, I aim to explore some novel approaches that can address the fundamental challenges around rapid aptamer probe-based detection and make the approach reliable and broadly applicable against any microbe (WP1 and WP2). I will also explore a novel approach for the detection of microbial activity (WP3). The experience of working in Cromerix, a start-up, will be the first of its kind for me as I have never worked in a commercial environment. Unique personal development will be achieved through interactions with client organisations, who bring in specific requirements around the detection of microbes, biofilms and microbial activity. Initial interactions with them have helped in shaping up the problem definition and inspired the novel methods of addressing those problems that I will explore through this project. I also look forward to gaining an exposure of the Alderley Park facilities where Cromerix has been offered a hotdesk. Success of this project will establish the proof of concept of novel rapid methods for broad detection of microbes and microbial activity for the first time. These methods have wide applicability in industry, clinic and home. Thus, this project aligns well with three of the four themes of the Industrial Strategy Challenge Fund: Ageing Society (Accelerating detection of disease, Leading-edge healthcare), Future of mobility (Future flight) and Clean Growth (Transforming food production).
Collaborator Contribution The following outcomes are expected as a result of this visit/placement. 1. New Skills and Knowledge - I will develop new knowledge around the feasibility of some novel generic methods for rapid broad detection of microbes and microbial activity using a. single aptamer probe (WP1), b. dual aptamer probe (WP1), c. catalytic transduction (WP2), and d. enzymatic cleavage (WP3). - I will learn how to liaise, communicate, present and submit reports to industrial clients. - As I aim to progress to the next phase of development working on the successful outcomes of this project, I will gain experience of writing collaborative proposals to funders such as Innovate-UK by working together with the clients. - As an early member of the start-up, I will gain exposure to the key small business operations, including purchase ordering, safety, and intellectual property management. 2. Collaborations This project proposal is guided by the "problem definition" discussions (scoping meetings) Cromerix has had with two established global players in the field of air transport (for microbial biofilms detection) and personal care (for microbial activity detection), where I have participated. The work I will carry out in this project will strengthen these collaborations and potentially lead to investments from these organisations for further technology development, an interest for which has been expressed by these organisations. Successful validation of the proposed generic methods will also allow me to approach organisations in other sectors of industry and clinic to develop new collaborations. 3. Impact Successful validation in this project will lead to further technology development to progress along the TRL and produce workable prototypes in collaboration with the industry partners. The methods being explored have broad applications. The chosen applications are based on interests received from industry players in niche sectors and can be extended to broader sectors of industry, clinic and consumer market. Thus, successful validation and TRL progression based on these methods can be potentially deliver a broad socio-economic impact through new product development around the detection of microbes and microbial activity. I look forward to working with the Alderley Park network as part of Cromerix in creating these translational impacts.
Impact Feedback from academic: I have met and exceeded my objectives of the FTMA project through the following outcomes. Skills and Knowledge The FTMA project gave me a unique experience of working in a start-up environment, which I found different from the academic research experience in many ways. The projects here are more industrial problem-driven rather than curiosity-driven as in a university. There is a greater importance on freedom to operate check before working on an idea. I helped the company Director with background IP search for discussion with the IP attorney, which helped ascertain the freedom to operate of the research I conducted in FTMA. My attending of the client meetings with the Director helped me understand how professionally these meetings are conducted, how NDAs are prepared and signed prior to the first meeting, how presentations are done, and how a problem is defined that is of importance to the client and deliverable within the required timeline, costs, and capacities of a start-up company. Working with the Director, I also learnt how to write industry-focused technical consulting and development proposals, two of which were submitted to clients, and one was submitted to Innovate UK. I have also gained some insights into the importance and challenges of contract negotiations before a project can actually start. The company was supported by NBIC's Accelerator funding for participating in the Alderley Park Accelerator. The Director primarily attended this, but I had the opportunity of getting introduced to the concepts of customer discovery, business plan proposition, and Lean innovation as these are now part of the company's knowledge base and have defined how we as a company approach business development. As an early member of the start-up, I have also gained exposure to the purchase and sales processes and setting up safety and risk assessment documents. Research outcomes For bacterial detection, the key outcomes included • Conserved epitopes for bacteria and novel aptamer beacon probes for broad detection of gram-negative bacteria that are applicable in various fields, including but not restricted to bacterial contamination in industry and environment, and clinical diagnostics of bacterial infections. • A novel method to detect viable bacteria using dual aptamer probes that is applicable for viability detection of other microbes, including fungi. • Conserved proteases and peptide probe for detection of bacterial activity. • A novel colorimetric transduction method for reader-less visual detection using peptide and aptamer probes. This method can be extended to detection of targets beyond bacteria. For fungal detection, the key outcomes included • Conserved epitopes and aptamers against these conserved epitopes with information on regions of interactions, which is now being used to design aptamer beacons for broad fungal detection as part of a client-funded project. For malodour detection and inhibition, the key outcomes included • Identifying the mechanism of malodour production by bacteria secreted from the human axillary glands. • Methods of detection of PatB, an enzyme associated with malodour production, detection of PatB activity and detection of malodour molecules produced by the bacteria. • A method and aptamer for inhibition of production of malodour molecules. Collaborations The outcomes of this FTMA project supported the progression of discussions with two clients, one in a niche field of microbial detection and another in personal care/microbiome. This involved submission of proposals to these organisations. The proposal to the first client (microbial detection) has been accepted after several rounds of discussion and has progressed towards contract negotiation phase. The project is scheduled to commence in August 2022 and will employ me. Progression with the proposal submitted to the second client (personal care/microbiome) has been put on hold by them, reportedly due to their organisational changes. Even if the second client is unable to progress, the outcome should help Cromerix to progress a collaboration on this topic with another organisation in the field of personal care/microbiome. In addition to the abovementioned collaboration, the outcomes of the project have also helped the company to engage with a wider network of stakeholders in the field of microbial detection, which is expected to drive more innovation-led projects supporting technology and business development. Impact The work done in the FTMA project is creating impact through multiple channels. The research outcomes have helped establish a client project in a niche field of microbial contamination detection, which is going to commence from August 2022, and has set strong foundations for a project in the field of personal care/microbiome. The outcomes also formed the basis of a recent application to the Fast Start Innovate-UK grant in the field of diabetic foot ulcer prognostic. All these projects have technology and business plan development in scope to support the eventual commercial success of the company. The outcomes are also supporting the growth in network and visibility of the company among the relevant stakeholders. Eventual adoption of the developed microbial detection technologies by the industry or clinic will address multiple themes of the Industrial Strategy Challenge Fund, some of these being Ageing Society (Accelerating detection of disease, Leading-edge healthcare), Future of mobility (Future flight) and Clean Growth (Transforming food production). With an outstanding support from the Director, the project has helped my personal development through the first and rich exposure to working in a business environment. The varied professional skills and knowledge gained around business case preparation, product development, IP, legal, safety and operations will help me contribute much better than before in my role as a "Scientist - Technology Development" in Cromerix. INTELLECTUAL PROPERTY In the near future, I will work closely with the IP attorney on patent filing around the method and materials developed within the FTMA project after doing a patentability assessment and IP strategy development together with the Director. WHAT DID HAPPEN NEXT? OR IS GOING TO HAPPEN NEXT? As mentioned above, the immediate next step is to work on a client project for microbial detection technology development in a niche space. Through this project, I will keep working with the team Cromerix on microbial detection technology development and progression towards commercialisation. Any support through direct funding from NBIC in a future proof of concept (POC) project or cash/in-kind support towards a funding application to another organisation for advancing the TRL of the technology will be vitally important and gratefully received. This FTMA funding was a unique support towards my gaining exposure in a technology start-up environment, which as an innovator I was aiming to get straight after my PhD. In addition to that, I am also getting a position in the company as a full-time employee. The funding also helped the company generate its own IP, which is acting as a foundation to its first projects and proposals. However, further data need to be generated before these IP can be filed as a patent. I hope we can apply for funding support from NBIC for patent filing at the time when it is needed. Also, any support towards my continued professional development (CPD), either through courses within NBIC or funding support to attend these externally will be very helpful and much appreciated. I would like to extend my sincerest thanks to the FTMA funding, which has helped unleash the potential in me and the company to take the first steps towards creating valuable impact to UK's society, economy, and environment.
Start Year 2021
 
Description NBIC FTMA Placement 20_IP_085 Novel rapid methods for broad detection of microbes and microbial activity (Praveen Kaveri) 
Organisation National Biofilms Innovation Centre
Sector Private 
PI Contribution My PhD research experience at Loughborough University showed that while it is possible to detect bacteria in principle in a single step using an aptamer probe, there are several challenges to address before this can be successfully applied in practice. In this project, I aim to explore some novel approaches that can address the fundamental challenges around rapid aptamer probe-based detection and make the approach reliable and broadly applicable against any microbe (WP1 and WP2). I will also explore a novel approach for the detection of microbial activity (WP3). The experience of working in Cromerix, a start-up, will be the first of its kind for me as I have never worked in a commercial environment. Unique personal development will be achieved through interactions with client organisations, who bring in specific requirements around the detection of microbes, biofilms and microbial activity. Initial interactions with them have helped in shaping up the problem definition and inspired the novel methods of addressing those problems that I will explore through this project. I also look forward to gaining an exposure of the Alderley Park facilities where Cromerix has been offered a hotdesk. Success of this project will establish the proof of concept of novel rapid methods for broad detection of microbes and microbial activity for the first time. These methods have wide applicability in industry, clinic and home. Thus, this project aligns well with three of the four themes of the Industrial Strategy Challenge Fund: Ageing Society (Accelerating detection of disease, Leading-edge healthcare), Future of mobility (Future flight) and Clean Growth (Transforming food production).
Collaborator Contribution The following outcomes are expected as a result of this visit/placement. 1. New Skills and Knowledge - I will develop new knowledge around the feasibility of some novel generic methods for rapid broad detection of microbes and microbial activity using a. single aptamer probe (WP1), b. dual aptamer probe (WP1), c. catalytic transduction (WP2), and d. enzymatic cleavage (WP3). - I will learn how to liaise, communicate, present and submit reports to industrial clients. - As I aim to progress to the next phase of development working on the successful outcomes of this project, I will gain experience of writing collaborative proposals to funders such as Innovate-UK by working together with the clients. - As an early member of the start-up, I will gain exposure to the key small business operations, including purchase ordering, safety, and intellectual property management. 2. Collaborations This project proposal is guided by the "problem definition" discussions (scoping meetings) Cromerix has had with two established global players in the field of air transport (for microbial biofilms detection) and personal care (for microbial activity detection), where I have participated. The work I will carry out in this project will strengthen these collaborations and potentially lead to investments from these organisations for further technology development, an interest for which has been expressed by these organisations. Successful validation of the proposed generic methods will also allow me to approach organisations in other sectors of industry and clinic to develop new collaborations. 3. Impact Successful validation in this project will lead to further technology development to progress along the TRL and produce workable prototypes in collaboration with the industry partners. The methods being explored have broad applications. The chosen applications are based on interests received from industry players in niche sectors and can be extended to broader sectors of industry, clinic and consumer market. Thus, successful validation and TRL progression based on these methods can be potentially deliver a broad socio-economic impact through new product development around the detection of microbes and microbial activity. I look forward to working with the Alderley Park network as part of Cromerix in creating these translational impacts.
Impact Feedback from academic: I have met and exceeded my objectives of the FTMA project through the following outcomes. Skills and Knowledge The FTMA project gave me a unique experience of working in a start-up environment, which I found different from the academic research experience in many ways. The projects here are more industrial problem-driven rather than curiosity-driven as in a university. There is a greater importance on freedom to operate check before working on an idea. I helped the company Director with background IP search for discussion with the IP attorney, which helped ascertain the freedom to operate of the research I conducted in FTMA. My attending of the client meetings with the Director helped me understand how professionally these meetings are conducted, how NDAs are prepared and signed prior to the first meeting, how presentations are done, and how a problem is defined that is of importance to the client and deliverable within the required timeline, costs, and capacities of a start-up company. Working with the Director, I also learnt how to write industry-focused technical consulting and development proposals, two of which were submitted to clients, and one was submitted to Innovate UK. I have also gained some insights into the importance and challenges of contract negotiations before a project can actually start. The company was supported by NBIC's Accelerator funding for participating in the Alderley Park Accelerator. The Director primarily attended this, but I had the opportunity of getting introduced to the concepts of customer discovery, business plan proposition, and Lean innovation as these are now part of the company's knowledge base and have defined how we as a company approach business development. As an early member of the start-up, I have also gained exposure to the purchase and sales processes and setting up safety and risk assessment documents. Research outcomes For bacterial detection, the key outcomes included • Conserved epitopes for bacteria and novel aptamer beacon probes for broad detection of gram-negative bacteria that are applicable in various fields, including but not restricted to bacterial contamination in industry and environment, and clinical diagnostics of bacterial infections. • A novel method to detect viable bacteria using dual aptamer probes that is applicable for viability detection of other microbes, including fungi. • Conserved proteases and peptide probe for detection of bacterial activity. • A novel colorimetric transduction method for reader-less visual detection using peptide and aptamer probes. This method can be extended to detection of targets beyond bacteria. For fungal detection, the key outcomes included • Conserved epitopes and aptamers against these conserved epitopes with information on regions of interactions, which is now being used to design aptamer beacons for broad fungal detection as part of a client-funded project. For malodour detection and inhibition, the key outcomes included • Identifying the mechanism of malodour production by bacteria secreted from the human axillary glands. • Methods of detection of PatB, an enzyme associated with malodour production, detection of PatB activity and detection of malodour molecules produced by the bacteria. • A method and aptamer for inhibition of production of malodour molecules. Collaborations The outcomes of this FTMA project supported the progression of discussions with two clients, one in a niche field of microbial detection and another in personal care/microbiome. This involved submission of proposals to these organisations. The proposal to the first client (microbial detection) has been accepted after several rounds of discussion and has progressed towards contract negotiation phase. The project is scheduled to commence in August 2022 and will employ me. Progression with the proposal submitted to the second client (personal care/microbiome) has been put on hold by them, reportedly due to their organisational changes. Even if the second client is unable to progress, the outcome should help Cromerix to progress a collaboration on this topic with another organisation in the field of personal care/microbiome. In addition to the abovementioned collaboration, the outcomes of the project have also helped the company to engage with a wider network of stakeholders in the field of microbial detection, which is expected to drive more innovation-led projects supporting technology and business development. Impact The work done in the FTMA project is creating impact through multiple channels. The research outcomes have helped establish a client project in a niche field of microbial contamination detection, which is going to commence from August 2022, and has set strong foundations for a project in the field of personal care/microbiome. The outcomes also formed the basis of a recent application to the Fast Start Innovate-UK grant in the field of diabetic foot ulcer prognostic. All these projects have technology and business plan development in scope to support the eventual commercial success of the company. The outcomes are also supporting the growth in network and visibility of the company among the relevant stakeholders. Eventual adoption of the developed microbial detection technologies by the industry or clinic will address multiple themes of the Industrial Strategy Challenge Fund, some of these being Ageing Society (Accelerating detection of disease, Leading-edge healthcare), Future of mobility (Future flight) and Clean Growth (Transforming food production). With an outstanding support from the Director, the project has helped my personal development through the first and rich exposure to working in a business environment. The varied professional skills and knowledge gained around business case preparation, product development, IP, legal, safety and operations will help me contribute much better than before in my role as a "Scientist - Technology Development" in Cromerix. INTELLECTUAL PROPERTY In the near future, I will work closely with the IP attorney on patent filing around the method and materials developed within the FTMA project after doing a patentability assessment and IP strategy development together with the Director. WHAT DID HAPPEN NEXT? OR IS GOING TO HAPPEN NEXT? As mentioned above, the immediate next step is to work on a client project for microbial detection technology development in a niche space. Through this project, I will keep working with the team Cromerix on microbial detection technology development and progression towards commercialisation. Any support through direct funding from NBIC in a future proof of concept (POC) project or cash/in-kind support towards a funding application to another organisation for advancing the TRL of the technology will be vitally important and gratefully received. This FTMA funding was a unique support towards my gaining exposure in a technology start-up environment, which as an innovator I was aiming to get straight after my PhD. In addition to that, I am also getting a position in the company as a full-time employee. The funding also helped the company generate its own IP, which is acting as a foundation to its first projects and proposals. However, further data need to be generated before these IP can be filed as a patent. I hope we can apply for funding support from NBIC for patent filing at the time when it is needed. Also, any support towards my continued professional development (CPD), either through courses within NBIC or funding support to attend these externally will be very helpful and much appreciated. I would like to extend my sincerest thanks to the FTMA funding, which has helped unleash the potential in me and the company to take the first steps towards creating valuable impact to UK's society, economy, and environment.
Start Year 2021
 
Description NBIC FTMA Placement P_19_05 Improving risk assessment methods for Microbially Influenced Corrosion (MIC) (Jeremy Webb) 
Organisation DNV GL
Country Norway 
Sector Private 
PI Contribution The cost of Microbial Induced Corrosion (MIC) in 2001 to the oil and gas industry was estimated to be between $3-7 Billion and therefore innovation in this area stands to provide significant economic benefit to the energy sector and better enable the UK to prosper from the energy revolution. MIC exerts its impact through damage to pipelines, equipment and products. A range of risk factors are currently used by field operators to predict when biocides and other interventions should be used to mitigate MIC. However, DNV GL and the University of Southampton currently consider the current risk factors to be insufficient to adequately predict equipment and pipeline failure. In this secondment DNV GL and the University of Southampton will further develop project plans to reduce the impact of MIC through innovative biofilm modelling and bioinformatic approaches. Applications across the oil, gas and renewable energy sectors will benefit from prolonged equipment life span, reduced product deterioration and improved biocide regimes, thereby meeting the challenge of 'Prospering from the energy revolution'. This project will build upon Robert Hull's experience in oil microbiology and industrial biofilm technologies. We will develop at least two separate research proposals during this secondment, facilitated through Robert Hull visiting plants and pipelines to identify challenges and carry out proof of concept work. Through the proof of concept work, Robert will gain further expertise in field based DNA sequencing and the use of the Nanopore sequencing platform. Furthermore, the secondment will facilitate the development of industry links, both in the UK and internationally through DNV GL's global research team. Robert would also have the opportunity to take advantage of DNV GL's extensive training program in areas including energy infrastructure and clean energy, gaining valuable expertise aligned with the 'Prospering from the energy revolution'.
Collaborator Contribution Robert Hull will be placed with DNV GL to better understand the processes and operations impacted by biofilm associated MIC in the oil, gas and renewables industry. This will involve training in plant and pipeline operations, and taking part in equipment failure investigations linked to biocorrosion in a 'real world' setting. While at DNV GL, Robert will provide microbiological and biofilm expertise to the company. Industry and particularly DNV GL will benefit from nurturing a centre of microbial corrosion expertise located within England. At present such expertise is associated with the offshore North Sea oil and gas industry centres such as Aberdeen. However, we are increasingly encountering microbial corrosion issues due to biofilm formation within equipment such as wind turbines and onshore assets such as heat exchangers, buried pipelines and high purity water systems. There is also a concern that the utilisation of hydrogen within the UK energy mix would encourage bacterial issues in areas such as underground gas storage. It would be valuable to increase the visibility of such assets within the academic community to facilitate initiation of projects in these areas. DNV GL and the University of Southampton team intend to take forward several plans, supported through this initial FTMA2 collaboration. Future outputs will include joint grant applications and research projects to combat MIC, with the benefits of the site visits and observations made in this secondment. Future research would also look to incorporate a MIC based PhD studentship supported by the University of Southampton, DNV GL and VIA University College. Research outputs would be detailed in joint publications in academic journals. The research from these projects would contribute towards the development of new industry standards for MIC risk assessment and biocide use, leading to reduced costs and enhanced equipment lifespan. These measures have the potential to make investments in energy generation more cost effective, facilitating additional growth in the UK energy sector. Current projects are based on the UK based Oxford Nanopore sequencing platform. Should these projects lead to the adoption of new sequencing based standards by the energy sector, we would expect additional growth by Oxford Nanopore and the creation of new services companies to carry out the sequencing for energy companies. The training carried out, networking opportunities taken and grant applications produced will be detailed in the NBIC final report.
Impact None yet.
Start Year 2020
 
Description NBIC FTMA Placement P_19_05 Improving risk assessment methods for Microbially Influenced Corrosion (MIC) (Jeremy Webb) 
Organisation National Biofilms Innovation Centre
Sector Private 
PI Contribution The cost of Microbial Induced Corrosion (MIC) in 2001 to the oil and gas industry was estimated to be between $3-7 Billion and therefore innovation in this area stands to provide significant economic benefit to the energy sector and better enable the UK to prosper from the energy revolution. MIC exerts its impact through damage to pipelines, equipment and products. A range of risk factors are currently used by field operators to predict when biocides and other interventions should be used to mitigate MIC. However, DNV GL and the University of Southampton currently consider the current risk factors to be insufficient to adequately predict equipment and pipeline failure. In this secondment DNV GL and the University of Southampton will further develop project plans to reduce the impact of MIC through innovative biofilm modelling and bioinformatic approaches. Applications across the oil, gas and renewable energy sectors will benefit from prolonged equipment life span, reduced product deterioration and improved biocide regimes, thereby meeting the challenge of 'Prospering from the energy revolution'. This project will build upon Robert Hull's experience in oil microbiology and industrial biofilm technologies. We will develop at least two separate research proposals during this secondment, facilitated through Robert Hull visiting plants and pipelines to identify challenges and carry out proof of concept work. Through the proof of concept work, Robert will gain further expertise in field based DNA sequencing and the use of the Nanopore sequencing platform. Furthermore, the secondment will facilitate the development of industry links, both in the UK and internationally through DNV GL's global research team. Robert would also have the opportunity to take advantage of DNV GL's extensive training program in areas including energy infrastructure and clean energy, gaining valuable expertise aligned with the 'Prospering from the energy revolution'.
Collaborator Contribution Robert Hull will be placed with DNV GL to better understand the processes and operations impacted by biofilm associated MIC in the oil, gas and renewables industry. This will involve training in plant and pipeline operations, and taking part in equipment failure investigations linked to biocorrosion in a 'real world' setting. While at DNV GL, Robert will provide microbiological and biofilm expertise to the company. Industry and particularly DNV GL will benefit from nurturing a centre of microbial corrosion expertise located within England. At present such expertise is associated with the offshore North Sea oil and gas industry centres such as Aberdeen. However, we are increasingly encountering microbial corrosion issues due to biofilm formation within equipment such as wind turbines and onshore assets such as heat exchangers, buried pipelines and high purity water systems. There is also a concern that the utilisation of hydrogen within the UK energy mix would encourage bacterial issues in areas such as underground gas storage. It would be valuable to increase the visibility of such assets within the academic community to facilitate initiation of projects in these areas. DNV GL and the University of Southampton team intend to take forward several plans, supported through this initial FTMA2 collaboration. Future outputs will include joint grant applications and research projects to combat MIC, with the benefits of the site visits and observations made in this secondment. Future research would also look to incorporate a MIC based PhD studentship supported by the University of Southampton, DNV GL and VIA University College. Research outputs would be detailed in joint publications in academic journals. The research from these projects would contribute towards the development of new industry standards for MIC risk assessment and biocide use, leading to reduced costs and enhanced equipment lifespan. These measures have the potential to make investments in energy generation more cost effective, facilitating additional growth in the UK energy sector. Current projects are based on the UK based Oxford Nanopore sequencing platform. Should these projects lead to the adoption of new sequencing based standards by the energy sector, we would expect additional growth by Oxford Nanopore and the creation of new services companies to carry out the sequencing for energy companies. The training carried out, networking opportunities taken and grant applications produced will be detailed in the NBIC final report.
Impact None yet.
Start Year 2020
 
Description NBIC FTMA Placement P_19_05 Improving risk assessment methods for Microbially Influenced Corrosion (MIC) (Jeremy Webb) 
Organisation University of Southampton
Country United Kingdom 
Sector Academic/University 
PI Contribution The cost of Microbial Induced Corrosion (MIC) in 2001 to the oil and gas industry was estimated to be between $3-7 Billion and therefore innovation in this area stands to provide significant economic benefit to the energy sector and better enable the UK to prosper from the energy revolution. MIC exerts its impact through damage to pipelines, equipment and products. A range of risk factors are currently used by field operators to predict when biocides and other interventions should be used to mitigate MIC. However, DNV GL and the University of Southampton currently consider the current risk factors to be insufficient to adequately predict equipment and pipeline failure. In this secondment DNV GL and the University of Southampton will further develop project plans to reduce the impact of MIC through innovative biofilm modelling and bioinformatic approaches. Applications across the oil, gas and renewable energy sectors will benefit from prolonged equipment life span, reduced product deterioration and improved biocide regimes, thereby meeting the challenge of 'Prospering from the energy revolution'. This project will build upon Robert Hull's experience in oil microbiology and industrial biofilm technologies. We will develop at least two separate research proposals during this secondment, facilitated through Robert Hull visiting plants and pipelines to identify challenges and carry out proof of concept work. Through the proof of concept work, Robert will gain further expertise in field based DNA sequencing and the use of the Nanopore sequencing platform. Furthermore, the secondment will facilitate the development of industry links, both in the UK and internationally through DNV GL's global research team. Robert would also have the opportunity to take advantage of DNV GL's extensive training program in areas including energy infrastructure and clean energy, gaining valuable expertise aligned with the 'Prospering from the energy revolution'.
Collaborator Contribution Robert Hull will be placed with DNV GL to better understand the processes and operations impacted by biofilm associated MIC in the oil, gas and renewables industry. This will involve training in plant and pipeline operations, and taking part in equipment failure investigations linked to biocorrosion in a 'real world' setting. While at DNV GL, Robert will provide microbiological and biofilm expertise to the company. Industry and particularly DNV GL will benefit from nurturing a centre of microbial corrosion expertise located within England. At present such expertise is associated with the offshore North Sea oil and gas industry centres such as Aberdeen. However, we are increasingly encountering microbial corrosion issues due to biofilm formation within equipment such as wind turbines and onshore assets such as heat exchangers, buried pipelines and high purity water systems. There is also a concern that the utilisation of hydrogen within the UK energy mix would encourage bacterial issues in areas such as underground gas storage. It would be valuable to increase the visibility of such assets within the academic community to facilitate initiation of projects in these areas. DNV GL and the University of Southampton team intend to take forward several plans, supported through this initial FTMA2 collaboration. Future outputs will include joint grant applications and research projects to combat MIC, with the benefits of the site visits and observations made in this secondment. Future research would also look to incorporate a MIC based PhD studentship supported by the University of Southampton, DNV GL and VIA University College. Research outputs would be detailed in joint publications in academic journals. The research from these projects would contribute towards the development of new industry standards for MIC risk assessment and biocide use, leading to reduced costs and enhanced equipment lifespan. These measures have the potential to make investments in energy generation more cost effective, facilitating additional growth in the UK energy sector. Current projects are based on the UK based Oxford Nanopore sequencing platform. Should these projects lead to the adoption of new sequencing based standards by the energy sector, we would expect additional growth by Oxford Nanopore and the creation of new services companies to carry out the sequencing for energy companies. The training carried out, networking opportunities taken and grant applications produced will be detailed in the NBIC final report.
Impact None yet.
Start Year 2020
 
Description NBIC FTMA Placement P_19_08 Assessment of the surface cleaning efficiency of Steam-e devices against environmental microbial contamination and biofilms (Nany Malissa Rahimi) 
Organisation National Biofilms Innovation Centre
Sector Private 
PI Contribution In this placement, I will spend three months working for Steam-e to provide a preliminary assessment of their novel and patented cleaning technology. Steam-e have developed a range of cleaning devices to enable rapid and efficient decontamination for a variety of environments. The technology works by using recirculated cold water that passes through a handheld fluent heater, which provides instant heating to 156°C. This results in directed steam production at a temperature of 115°C. To date, the portable devices have been designed to remove chewing gum, or act as a general purpose steam cleaner for commercial cleaning. Due to the safe, rapid and efficient design of the cleaning process, I will be investigating whether the technology can provide effective cleaning and decontamination of microbial populations including biofilms. To do this, I will use a series of indicator organisms to initially assess whether the steam treatment inactivates the key bacteria (Gram negative and Gram positive), including spore-forming species. The species to be focused on will include: Klebsiella pneumoniae, MRSA and Clostridium difficile (all important hospital pathogens); Salomonella spp. and Listeria monocytogenes (important in food production); and the industry standard indicator Geobacillus stearothermophilus. Following this, the action of the device on biofilms will be assessed. This work will provide experience on standard testing as required for product development and evaluation. Throughout, I will be working alongside Steam-e engineers and company representatives, providing me with commercial experience and insight into product design and improvement. I will also learn about market analysis and gain understanding on the challenges encountered with different markets; NHS, food production and other regulated industries.
Collaborator Contribution Steam-e has not explored antimicrobial markets and, if successful, a range of devices could be developed following this initial phase of testing. The problems of antimicrobial resistance and effective cleaning are major global challenges, with the latter having a clear impact on controlling the former by improved decontamination. Improved cleaning has implications in a wide range of industries and environments, not least food production and handling, and infection prevention both for animal husbandry and in healthcare facilities. The broad scope and potential for this technology has important socio-economic impact with global reach. Considering infection prevention in healthcare facilities and hospitals, we know environmental contamination is a key driver in continued healthcare associated infections, and a technology which provides rapid and effective cleaning would be highly beneficial. The ability to effectively clean following bacterial (including spore-forming), viral and fungal outbreaks, in a simple way, minimizing ward closure times would improve patient outcomes as well as having considerable financial savings. Following this project, the team would apply for further funding to allow more detailed testing and scaleup. Depending on the application, this funding could be from the NIHR i4i programme, allowing clinical evaluation and testing. The team has strong links to University Hospital Southampton and the Infection Prevention team, as well as Wessex Academic Health Services Network. Within the food and agri-tech industries, the team have further links to facilities that could offer subsequent pilot testing, with funding potential from BBSRC and Innovate identified. Initially, the placement results will be used to produce a final report to inform product design and development. With the consent of all parties, if appropriate, data will be used for a scientific paper and presented at appropriate conferences. The intention is for the placement to lead to a programme of collaborative work between Steam-e, the University of Southampton and NBIC.
Impact Feedback from academic: This project has given insight from the research conducted against biofilms, which added value to their market analysis. It has expanded my commercial experience in also providing feedbacks into decontamination product development. To date, the Steam-E which have been designed to remove chewing gum and used as a general-purpose steam cleaner for commercial cleaning. Therefore, the study evaluates the antibacterial potential of Gum-E as a disinfection machine in managing and preventing biofilm infection. The testing was conducted on three different biofilm ages (24h, 48h and 7d) and further categorised into clean and dirty. These biofilm parameters were used to see if there would be any log reduction after treatment times of 5mins, 10s, 5s, 3s, and 1s with Gum-E. The preliminary study on harvested biofilm from the control SS coupons (without steam treatment) when analysed using two-way ANOVA showed that there are significant differences between clean and dirty biofilm aged 24h (p < 0.0001) and 7d (P=0.0003), but not for 48h (p=0.2820). Overall, all biofilm types and ages showed total reduction (100%) with 10s and 5mins of Gum-E treatment. The 5s treatment were able to give total reduction (100%) in the 24h dirty biofilm sample compared to 24h clean biofilm which showed 4-log reduction (99.99%). In 48h sample, the 5s treatment were able to give 3.9-log reduction in clean compared to dirty with 4.4-log reduction. In 7d sample, total reduction (100%) were achieved with 5s treatment for both dirty and clean biofilm sample. Overall, for each treatment of 1s, 3s and 5s showed no significant differences on the log reduction between clean and dirty samples (p>0.9999). The 1s of Gum-E treatment showed a minimum of 2-log reduction (99%) in the 7d dirty sample and a maximum of 3-log reduction (99.9%) in the 24h dirty sample. This shows that Gum-E is an effective and rapid cleaning device that is not affected by the presence of organic matter. Biofilms has been a major prevalent cause in hospital-acquired infections (HAI) and this research has shown that Gum-E machine is able to eliminate if not reduce this risk if used by health partners like the NHS as well as it being useful in food production and other regulated industries. This is also in addition to the recent global COVID19, the Gum-E has the potential in being a portable decontamination machine.
Start Year 2019
 
Description NBIC FTMA Placement P_19_08 Assessment of the surface cleaning efficiency of Steam-e devices against environmental microbial contamination and biofilms (Nany Malissa Rahimi) 
Organisation Steam-e Holdings Limited
Country United Kingdom 
Sector Private 
PI Contribution In this placement, I will spend three months working for Steam-e to provide a preliminary assessment of their novel and patented cleaning technology. Steam-e have developed a range of cleaning devices to enable rapid and efficient decontamination for a variety of environments. The technology works by using recirculated cold water that passes through a handheld fluent heater, which provides instant heating to 156°C. This results in directed steam production at a temperature of 115°C. To date, the portable devices have been designed to remove chewing gum, or act as a general purpose steam cleaner for commercial cleaning. Due to the safe, rapid and efficient design of the cleaning process, I will be investigating whether the technology can provide effective cleaning and decontamination of microbial populations including biofilms. To do this, I will use a series of indicator organisms to initially assess whether the steam treatment inactivates the key bacteria (Gram negative and Gram positive), including spore-forming species. The species to be focused on will include: Klebsiella pneumoniae, MRSA and Clostridium difficile (all important hospital pathogens); Salomonella spp. and Listeria monocytogenes (important in food production); and the industry standard indicator Geobacillus stearothermophilus. Following this, the action of the device on biofilms will be assessed. This work will provide experience on standard testing as required for product development and evaluation. Throughout, I will be working alongside Steam-e engineers and company representatives, providing me with commercial experience and insight into product design and improvement. I will also learn about market analysis and gain understanding on the challenges encountered with different markets; NHS, food production and other regulated industries.
Collaborator Contribution Steam-e has not explored antimicrobial markets and, if successful, a range of devices could be developed following this initial phase of testing. The problems of antimicrobial resistance and effective cleaning are major global challenges, with the latter having a clear impact on controlling the former by improved decontamination. Improved cleaning has implications in a wide range of industries and environments, not least food production and handling, and infection prevention both for animal husbandry and in healthcare facilities. The broad scope and potential for this technology has important socio-economic impact with global reach. Considering infection prevention in healthcare facilities and hospitals, we know environmental contamination is a key driver in continued healthcare associated infections, and a technology which provides rapid and effective cleaning would be highly beneficial. The ability to effectively clean following bacterial (including spore-forming), viral and fungal outbreaks, in a simple way, minimizing ward closure times would improve patient outcomes as well as having considerable financial savings. Following this project, the team would apply for further funding to allow more detailed testing and scaleup. Depending on the application, this funding could be from the NIHR i4i programme, allowing clinical evaluation and testing. The team has strong links to University Hospital Southampton and the Infection Prevention team, as well as Wessex Academic Health Services Network. Within the food and agri-tech industries, the team have further links to facilities that could offer subsequent pilot testing, with funding potential from BBSRC and Innovate identified. Initially, the placement results will be used to produce a final report to inform product design and development. With the consent of all parties, if appropriate, data will be used for a scientific paper and presented at appropriate conferences. The intention is for the placement to lead to a programme of collaborative work between Steam-e, the University of Southampton and NBIC.
Impact Feedback from academic: This project has given insight from the research conducted against biofilms, which added value to their market analysis. It has expanded my commercial experience in also providing feedbacks into decontamination product development. To date, the Steam-E which have been designed to remove chewing gum and used as a general-purpose steam cleaner for commercial cleaning. Therefore, the study evaluates the antibacterial potential of Gum-E as a disinfection machine in managing and preventing biofilm infection. The testing was conducted on three different biofilm ages (24h, 48h and 7d) and further categorised into clean and dirty. These biofilm parameters were used to see if there would be any log reduction after treatment times of 5mins, 10s, 5s, 3s, and 1s with Gum-E. The preliminary study on harvested biofilm from the control SS coupons (without steam treatment) when analysed using two-way ANOVA showed that there are significant differences between clean and dirty biofilm aged 24h (p < 0.0001) and 7d (P=0.0003), but not for 48h (p=0.2820). Overall, all biofilm types and ages showed total reduction (100%) with 10s and 5mins of Gum-E treatment. The 5s treatment were able to give total reduction (100%) in the 24h dirty biofilm sample compared to 24h clean biofilm which showed 4-log reduction (99.99%). In 48h sample, the 5s treatment were able to give 3.9-log reduction in clean compared to dirty with 4.4-log reduction. In 7d sample, total reduction (100%) were achieved with 5s treatment for both dirty and clean biofilm sample. Overall, for each treatment of 1s, 3s and 5s showed no significant differences on the log reduction between clean and dirty samples (p>0.9999). The 1s of Gum-E treatment showed a minimum of 2-log reduction (99%) in the 7d dirty sample and a maximum of 3-log reduction (99.9%) in the 24h dirty sample. This shows that Gum-E is an effective and rapid cleaning device that is not affected by the presence of organic matter. Biofilms has been a major prevalent cause in hospital-acquired infections (HAI) and this research has shown that Gum-E machine is able to eliminate if not reduce this risk if used by health partners like the NHS as well as it being useful in food production and other regulated industries. This is also in addition to the recent global COVID19, the Gum-E has the potential in being a portable decontamination machine.
Start Year 2019
 
Description NBIC FTMA Placement P_19_08 Assessment of the surface cleaning efficiency of Steam-e devices against environmental microbial contamination and biofilms (Nany Malissa Rahimi) 
Organisation University of Southampton
Country United Kingdom 
Sector Academic/University 
PI Contribution In this placement, I will spend three months working for Steam-e to provide a preliminary assessment of their novel and patented cleaning technology. Steam-e have developed a range of cleaning devices to enable rapid and efficient decontamination for a variety of environments. The technology works by using recirculated cold water that passes through a handheld fluent heater, which provides instant heating to 156°C. This results in directed steam production at a temperature of 115°C. To date, the portable devices have been designed to remove chewing gum, or act as a general purpose steam cleaner for commercial cleaning. Due to the safe, rapid and efficient design of the cleaning process, I will be investigating whether the technology can provide effective cleaning and decontamination of microbial populations including biofilms. To do this, I will use a series of indicator organisms to initially assess whether the steam treatment inactivates the key bacteria (Gram negative and Gram positive), including spore-forming species. The species to be focused on will include: Klebsiella pneumoniae, MRSA and Clostridium difficile (all important hospital pathogens); Salomonella spp. and Listeria monocytogenes (important in food production); and the industry standard indicator Geobacillus stearothermophilus. Following this, the action of the device on biofilms will be assessed. This work will provide experience on standard testing as required for product development and evaluation. Throughout, I will be working alongside Steam-e engineers and company representatives, providing me with commercial experience and insight into product design and improvement. I will also learn about market analysis and gain understanding on the challenges encountered with different markets; NHS, food production and other regulated industries.
Collaborator Contribution Steam-e has not explored antimicrobial markets and, if successful, a range of devices could be developed following this initial phase of testing. The problems of antimicrobial resistance and effective cleaning are major global challenges, with the latter having a clear impact on controlling the former by improved decontamination. Improved cleaning has implications in a wide range of industries and environments, not least food production and handling, and infection prevention both for animal husbandry and in healthcare facilities. The broad scope and potential for this technology has important socio-economic impact with global reach. Considering infection prevention in healthcare facilities and hospitals, we know environmental contamination is a key driver in continued healthcare associated infections, and a technology which provides rapid and effective cleaning would be highly beneficial. The ability to effectively clean following bacterial (including spore-forming), viral and fungal outbreaks, in a simple way, minimizing ward closure times would improve patient outcomes as well as having considerable financial savings. Following this project, the team would apply for further funding to allow more detailed testing and scaleup. Depending on the application, this funding could be from the NIHR i4i programme, allowing clinical evaluation and testing. The team has strong links to University Hospital Southampton and the Infection Prevention team, as well as Wessex Academic Health Services Network. Within the food and agri-tech industries, the team have further links to facilities that could offer subsequent pilot testing, with funding potential from BBSRC and Innovate identified. Initially, the placement results will be used to produce a final report to inform product design and development. With the consent of all parties, if appropriate, data will be used for a scientific paper and presented at appropriate conferences. The intention is for the placement to lead to a programme of collaborative work between Steam-e, the University of Southampton and NBIC.
Impact Feedback from academic: This project has given insight from the research conducted against biofilms, which added value to their market analysis. It has expanded my commercial experience in also providing feedbacks into decontamination product development. To date, the Steam-E which have been designed to remove chewing gum and used as a general-purpose steam cleaner for commercial cleaning. Therefore, the study evaluates the antibacterial potential of Gum-E as a disinfection machine in managing and preventing biofilm infection. The testing was conducted on three different biofilm ages (24h, 48h and 7d) and further categorised into clean and dirty. These biofilm parameters were used to see if there would be any log reduction after treatment times of 5mins, 10s, 5s, 3s, and 1s with Gum-E. The preliminary study on harvested biofilm from the control SS coupons (without steam treatment) when analysed using two-way ANOVA showed that there are significant differences between clean and dirty biofilm aged 24h (p < 0.0001) and 7d (P=0.0003), but not for 48h (p=0.2820). Overall, all biofilm types and ages showed total reduction (100%) with 10s and 5mins of Gum-E treatment. The 5s treatment were able to give total reduction (100%) in the 24h dirty biofilm sample compared to 24h clean biofilm which showed 4-log reduction (99.99%). In 48h sample, the 5s treatment were able to give 3.9-log reduction in clean compared to dirty with 4.4-log reduction. In 7d sample, total reduction (100%) were achieved with 5s treatment for both dirty and clean biofilm sample. Overall, for each treatment of 1s, 3s and 5s showed no significant differences on the log reduction between clean and dirty samples (p>0.9999). The 1s of Gum-E treatment showed a minimum of 2-log reduction (99%) in the 7d dirty sample and a maximum of 3-log reduction (99.9%) in the 24h dirty sample. This shows that Gum-E is an effective and rapid cleaning device that is not affected by the presence of organic matter. Biofilms has been a major prevalent cause in hospital-acquired infections (HAI) and this research has shown that Gum-E machine is able to eliminate if not reduce this risk if used by health partners like the NHS as well as it being useful in food production and other regulated industries. This is also in addition to the recent global COVID19, the Gum-E has the potential in being a portable decontamination machine.
Start Year 2019
 
Description NBIC FTMA Placement P_19_2_38 Removal of Oral Biofilm by Activated Oxygen: A Targeted Study (Claudio Lourenco) 
Organisation GlaxoSmithKline (GSK)
Country Global 
Sector Private 
PI Contribution This industrial placement will provide an outstanding opportunity to apply all the knowledge and findings acquired during my EngD research degree at UCL. The aims and objectives of this project are to perform real time imaging of the removal of oral biofilm by reactive oxygen species (ROS) generated in denture cleansers. By employing scanning laser confocal microscopy with a three-dye combination, that will allow the visualization of the biofilm sugary matrix and its microorganisms and therefore, access which structures are being targeted by the ROS produced. In this research project, we aim to develop a dual species biofilm using Candida albicans and Streptococcus mutans. Both microorganisms are part of our microflora, C. albicans is the most prevalent cause of fungal infections in humans and when out of control, in the oral cavity, can cause several complications including candidiasis and stomatitis, Streptococcus mutans is a early colonizer of the mouth cavity, known to produce a polysaccharide matrix that acts as scaffolding for microorganisms that make the oral biofilm. This industrial placement is an excellent opportunity to carry out a set of unique inter-linking activities with private industry, allowing me to develop my research skills as an independent researcher to apply for further funding. This research project will provide new insights on the mode of action of ROS in the removal of biofilms and the killing of bacteria to prevent recurring oral complications plaguing denture wearers. Thus, this project is expected to have a remarkable international impact, attracting a wide interdisciplinary recognition and opening the door to future collaborations between GSK-CH and UCL by promoting the use of combined resources and knowledge exchange.
Collaborator Contribution In the initial stage of this project an oral presentation will be delivered, to share the major findings and techniques used throughout my EngD studies, to the relevant teams at the GSK-CH. I will be showing the effectiveness and advantages in the use of H-NMR and fluorimetry to screen existing and future formulations of denture cleansers. And at the same time show the capabilities of the equipment available at UCL and promote the exchange of resources and expertise between both institutions. On the societal aspect the relevance of this project arises from the fact that dentures are not only a concern for the elderly people. Recently the Oral Health Foundation has reported that 16% of the UK population wear dentures, including a million of people aged between 16 to 44. According with the Special Eurobarometer Oral Health denture wearers experience complications such as toothache, painful gums and sore spots that lead to chewing and biting problems affecting their diet and nutrition, as well as the aesthetic point of view, mainly cause by the growth of biofilm in dentures. In addition, the World Health Organization (WHO) has predicted that the number of patients requiring dentures worldwide will increase in the future years. In addition, recent studies suggests that the oral cavity microflora can contribute to systemic and chronic diseases but also more commonly for denture wearers denture associated stomatitis, caused mainly by an increase in Candida albicans, and mal odour caused by other microorganisms. Therefore, there is a significant need to improve the cleaning and disinfection mechanisms in oral care products for dentures. The main agent of our product is peracetic acid that is known to be a powerful bleaching and antimicrobial agent with a proven track of success. It has several applications ranging from disinfectant in hospitals to bleaching agent in household laundry. Therefore, in a near future the findings of this project can effectively impact other relevant areas where disinfection and removal of bacteria are a major concern. The development of this technology will provide fresh insight into existing infrastructure, attracting industrial interest that could lead to the development of commercial pathways for the promising formulations that will be developed in this industrial placement. Furthermore, a joint publication between UCL and GSK-CH on a peer reviewed journal will be drafted and the findings of this study will also be presented at a major international meeting.
Impact The main outcome of the project was the data concerning the effects of the pH on the generation of ROS in a simple formulation. This knowledge can be relevant when considering the development of novel formulations. The current formulations operate at a pH close to neutral that is within the optimal range for the reaction to take place in a effective way but a more acidic pH seemed to contribute for the stability of peracetic acid. As result of the work being conducted at UCL, the industrial partner GSK had more questions that needed answers and another project was funded and set up to study other formulations using the techniques developed throughout my EngD studies a UCL. A clear indicator of the success of the initial project and the advantages and quality of work conducted within the Department of Chemistry of UCL. Ideally the project must evolve for the next step that will be to observe how the pH effects towards the stability of peracetic acid will contribute to biofilm removal. In the case that it is more effective than the current formulations, that use neutral pH, a closer look and further investigations will be necessary. The fact that peracetic acid is widely used to sanitize and clean surfaces there is also the potential develop the product for other applications. I have recently left UCL therefore will not be possible for me to continue with this project but I am happy to contribute with my knowledge and expertise in the field if anyone in the future is willing to take up on this research.
Start Year 2020
 
Description NBIC FTMA Placement P_19_2_38 Removal of Oral Biofilm by Activated Oxygen: A Targeted Study (Claudio Lourenco) 
Organisation National Biofilms Innovation Centre
Sector Private 
PI Contribution This industrial placement will provide an outstanding opportunity to apply all the knowledge and findings acquired during my EngD research degree at UCL. The aims and objectives of this project are to perform real time imaging of the removal of oral biofilm by reactive oxygen species (ROS) generated in denture cleansers. By employing scanning laser confocal microscopy with a three-dye combination, that will allow the visualization of the biofilm sugary matrix and its microorganisms and therefore, access which structures are being targeted by the ROS produced. In this research project, we aim to develop a dual species biofilm using Candida albicans and Streptococcus mutans. Both microorganisms are part of our microflora, C. albicans is the most prevalent cause of fungal infections in humans and when out of control, in the oral cavity, can cause several complications including candidiasis and stomatitis, Streptococcus mutans is a early colonizer of the mouth cavity, known to produce a polysaccharide matrix that acts as scaffolding for microorganisms that make the oral biofilm. This industrial placement is an excellent opportunity to carry out a set of unique inter-linking activities with private industry, allowing me to develop my research skills as an independent researcher to apply for further funding. This research project will provide new insights on the mode of action of ROS in the removal of biofilms and the killing of bacteria to prevent recurring oral complications plaguing denture wearers. Thus, this project is expected to have a remarkable international impact, attracting a wide interdisciplinary recognition and opening the door to future collaborations between GSK-CH and UCL by promoting the use of combined resources and knowledge exchange.
Collaborator Contribution In the initial stage of this project an oral presentation will be delivered, to share the major findings and techniques used throughout my EngD studies, to the relevant teams at the GSK-CH. I will be showing the effectiveness and advantages in the use of H-NMR and fluorimetry to screen existing and future formulations of denture cleansers. And at the same time show the capabilities of the equipment available at UCL and promote the exchange of resources and expertise between both institutions. On the societal aspect the relevance of this project arises from the fact that dentures are not only a concern for the elderly people. Recently the Oral Health Foundation has reported that 16% of the UK population wear dentures, including a million of people aged between 16 to 44. According with the Special Eurobarometer Oral Health denture wearers experience complications such as toothache, painful gums and sore spots that lead to chewing and biting problems affecting their diet and nutrition, as well as the aesthetic point of view, mainly cause by the growth of biofilm in dentures. In addition, the World Health Organization (WHO) has predicted that the number of patients requiring dentures worldwide will increase in the future years. In addition, recent studies suggests that the oral cavity microflora can contribute to systemic and chronic diseases but also more commonly for denture wearers denture associated stomatitis, caused mainly by an increase in Candida albicans, and mal odour caused by other microorganisms. Therefore, there is a significant need to improve the cleaning and disinfection mechanisms in oral care products for dentures. The main agent of our product is peracetic acid that is known to be a powerful bleaching and antimicrobial agent with a proven track of success. It has several applications ranging from disinfectant in hospitals to bleaching agent in household laundry. Therefore, in a near future the findings of this project can effectively impact other relevant areas where disinfection and removal of bacteria are a major concern. The development of this technology will provide fresh insight into existing infrastructure, attracting industrial interest that could lead to the development of commercial pathways for the promising formulations that will be developed in this industrial placement. Furthermore, a joint publication between UCL and GSK-CH on a peer reviewed journal will be drafted and the findings of this study will also be presented at a major international meeting.
Impact The main outcome of the project was the data concerning the effects of the pH on the generation of ROS in a simple formulation. This knowledge can be relevant when considering the development of novel formulations. The current formulations operate at a pH close to neutral that is within the optimal range for the reaction to take place in a effective way but a more acidic pH seemed to contribute for the stability of peracetic acid. As result of the work being conducted at UCL, the industrial partner GSK had more questions that needed answers and another project was funded and set up to study other formulations using the techniques developed throughout my EngD studies a UCL. A clear indicator of the success of the initial project and the advantages and quality of work conducted within the Department of Chemistry of UCL. Ideally the project must evolve for the next step that will be to observe how the pH effects towards the stability of peracetic acid will contribute to biofilm removal. In the case that it is more effective than the current formulations, that use neutral pH, a closer look and further investigations will be necessary. The fact that peracetic acid is widely used to sanitize and clean surfaces there is also the potential develop the product for other applications. I have recently left UCL therefore will not be possible for me to continue with this project but I am happy to contribute with my knowledge and expertise in the field if anyone in the future is willing to take up on this research.
Start Year 2020
 
Description NBIC FTMA Placement P_19_2_38 Removal of Oral Biofilm by Activated Oxygen: A Targeted Study (Claudio Lourenco) 
Organisation University College London
Country United Kingdom 
Sector Academic/University 
PI Contribution This industrial placement will provide an outstanding opportunity to apply all the knowledge and findings acquired during my EngD research degree at UCL. The aims and objectives of this project are to perform real time imaging of the removal of oral biofilm by reactive oxygen species (ROS) generated in denture cleansers. By employing scanning laser confocal microscopy with a three-dye combination, that will allow the visualization of the biofilm sugary matrix and its microorganisms and therefore, access which structures are being targeted by the ROS produced. In this research project, we aim to develop a dual species biofilm using Candida albicans and Streptococcus mutans. Both microorganisms are part of our microflora, C. albicans is the most prevalent cause of fungal infections in humans and when out of control, in the oral cavity, can cause several complications including candidiasis and stomatitis, Streptococcus mutans is a early colonizer of the mouth cavity, known to produce a polysaccharide matrix that acts as scaffolding for microorganisms that make the oral biofilm. This industrial placement is an excellent opportunity to carry out a set of unique inter-linking activities with private industry, allowing me to develop my research skills as an independent researcher to apply for further funding. This research project will provide new insights on the mode of action of ROS in the removal of biofilms and the killing of bacteria to prevent recurring oral complications plaguing denture wearers. Thus, this project is expected to have a remarkable international impact, attracting a wide interdisciplinary recognition and opening the door to future collaborations between GSK-CH and UCL by promoting the use of combined resources and knowledge exchange.
Collaborator Contribution In the initial stage of this project an oral presentation will be delivered, to share the major findings and techniques used throughout my EngD studies, to the relevant teams at the GSK-CH. I will be showing the effectiveness and advantages in the use of H-NMR and fluorimetry to screen existing and future formulations of denture cleansers. And at the same time show the capabilities of the equipment available at UCL and promote the exchange of resources and expertise between both institutions. On the societal aspect the relevance of this project arises from the fact that dentures are not only a concern for the elderly people. Recently the Oral Health Foundation has reported that 16% of the UK population wear dentures, including a million of people aged between 16 to 44. According with the Special Eurobarometer Oral Health denture wearers experience complications such as toothache, painful gums and sore spots that lead to chewing and biting problems affecting their diet and nutrition, as well as the aesthetic point of view, mainly cause by the growth of biofilm in dentures. In addition, the World Health Organization (WHO) has predicted that the number of patients requiring dentures worldwide will increase in the future years. In addition, recent studies suggests that the oral cavity microflora can contribute to systemic and chronic diseases but also more commonly for denture wearers denture associated stomatitis, caused mainly by an increase in Candida albicans, and mal odour caused by other microorganisms. Therefore, there is a significant need to improve the cleaning and disinfection mechanisms in oral care products for dentures. The main agent of our product is peracetic acid that is known to be a powerful bleaching and antimicrobial agent with a proven track of success. It has several applications ranging from disinfectant in hospitals to bleaching agent in household laundry. Therefore, in a near future the findings of this project can effectively impact other relevant areas where disinfection and removal of bacteria are a major concern. The development of this technology will provide fresh insight into existing infrastructure, attracting industrial interest that could lead to the development of commercial pathways for the promising formulations that will be developed in this industrial placement. Furthermore, a joint publication between UCL and GSK-CH on a peer reviewed journal will be drafted and the findings of this study will also be presented at a major international meeting.
Impact The main outcome of the project was the data concerning the effects of the pH on the generation of ROS in a simple formulation. This knowledge can be relevant when considering the development of novel formulations. The current formulations operate at a pH close to neutral that is within the optimal range for the reaction to take place in a effective way but a more acidic pH seemed to contribute for the stability of peracetic acid. As result of the work being conducted at UCL, the industrial partner GSK had more questions that needed answers and another project was funded and set up to study other formulations using the techniques developed throughout my EngD studies a UCL. A clear indicator of the success of the initial project and the advantages and quality of work conducted within the Department of Chemistry of UCL. Ideally the project must evolve for the next step that will be to observe how the pH effects towards the stability of peracetic acid will contribute to biofilm removal. In the case that it is more effective than the current formulations, that use neutral pH, a closer look and further investigations will be necessary. The fact that peracetic acid is widely used to sanitize and clean surfaces there is also the potential develop the product for other applications. I have recently left UCL therefore will not be possible for me to continue with this project but I am happy to contribute with my knowledge and expertise in the field if anyone in the future is willing to take up on this research.
Start Year 2020
 
Description NBIC FTMA Placement P_19_2_44 Mechanical properties of biofilm on complex substrate (Zhenyu Zhang) 
Organisation National Biofilms Innovation Centre
Sector Private 
PI Contribution The aim of this project is to place Maria Masoura, a PhD student with microbiology background, in a world-leading chemical company, Procter & Gamble to: 1. establish appreciation of industrial perspectives of biofilm; 2. develop industrial links with P&G; 3. identify key parameters that manage and engineer biofilms on complex substrates. During the three months placement period, Maria plans to systematically investigate and measure microbial adhesion to model soils and the mechanical properties of the biofilm/soil complex consequently formed. The scientific nature of the proposal is aligned with at least two areas outlined in the ISCF: accelerating detection of disease, and healthy ageing. Biofilms represent a major concern in industry and hospital healthcare settings where they cause severe structural and health problems (Jose Luis Del Pozo 2017; Gibson et al. 1997). Nowadays, the majority of efforts to treat biofilms are based on antimicrobial agents, which has led to one of the biggest issue in the world that is the bacteria and microorganisms antimicrobial resistance (AMR). The decrease of the effectiveness of these agents poses a serious concern and new ways for tackling biofilms without using antimicrobials are needed. The quantification of adhesion and cohesion forces is crucial in understanding, predicting and modelling biofilm development and removal. The primary objective for the placement is to identify the models and characterize the system as a function of the environment, such as pH, Relative Humidity, temperature, ionic strength, and time. We wish to use the opportunity to exchange both personnel and knowledge between academic (UoB) and industrial sectors (P&G). The placement holder will be exposed to a modern cooperate training package such as management skills, technical skills such as bacteria growth and monitoring for chemical industry.
Collaborator Contribution It is anticipated that the outcomes of the planned exchange include the following: 1. New skills: to be transferred between the University of Birmingham to P&G. Maria will use her expertise in microbiology to analyse the presence of biofilms on substrates that are of interest to both industrial and household related applications. In return, Maria will have the opportunity to be trained on a variety of industrial protocols that are not available under an academic research settings. 2. Personal development: Maria will establish in-depth appreciation of industrial perspective on engineering and managing biofilm. Furthermore, she will develop connections with colleagues at P&G, which is tremendously invaluable to an early career researcher. Furthermore, Maria will be offered to attend a number of bespoke training courses by P&G to develop her transferrable skills such as Project Management. 3. New knowledge: concerning the presence and mechanical integrity of biofilms will be generated, which bears fundamental importance to both academic research and industrial impact. This links the past work over different length scales (Goode 2013, Pen 2011), alongside the industrial R&D work. 4. Immediate collaboration: upon success of the placement, whereby key parameters that influence the mechanical properties of biofilm are identified, P&G will commit a further £60k to systematically expand the investigation, as indicated in the supporting letter. This would also strengthen the connection between Maria and colleagues at P&G developed through the placement. 5. Future partnership: University of Birmingham has a strategic partnership with P&G, for the past two decades, with a focus on the Formulation Engineering related work. The proposed work is closely related to the development of new formulated products or processes, and opens new avenues for future collaboration. 6. Societal impact: The learning outcome will help to design new strategies in addressing biofilm related issues in household and healthcare environment. Its impact to our society will be amplified and implemented through large organisation such as P&G who has an established and closed loop in transferring knowledge to real time. We will also engage with other potential partners through the National Biofilm Innovation Centre, to not only disseminate the findings, but to prompt the protocols and techniques developed.
Impact Together with the industrial partners, Dr Masoura identified the skin microbes that are of interest to the particular end applications, and developed the corresponding biofilms on fabrics, which provided invaluable insight to the company in understanding the influence of microbiological activities on the development of fabric care products. A key outcome of the project was to identify the factor(s) that control the adhesion and cohesion of such biofilms - this could be used for the development of new technologies in preventing and removing biofilms from fabrics. Our industrial partner, P&G, was delighted by the initial results generated by Dr Masoura and committed a follow-on fund of £60k to support Maria as a Research Fellow at the University of Birmingham continuing her investigation with a systematic manner. No other support is required from the NBIC for this project. Dr Masoura had subsequently developed her connection with another SME using natural materials for food packaging, and plans to submit a research proposal in the upcoming round of funding call.
Start Year 2020
 
Description NBIC FTMA Placement P_19_2_44 Mechanical properties of biofilm on complex substrate (Zhenyu Zhang) 
Organisation Procter & Gamble
Country United States 
Sector Private 
PI Contribution The aim of this project is to place Maria Masoura, a PhD student with microbiology background, in a world-leading chemical company, Procter & Gamble to: 1. establish appreciation of industrial perspectives of biofilm; 2. develop industrial links with P&G; 3. identify key parameters that manage and engineer biofilms on complex substrates. During the three months placement period, Maria plans to systematically investigate and measure microbial adhesion to model soils and the mechanical properties of the biofilm/soil complex consequently formed. The scientific nature of the proposal is aligned with at least two areas outlined in the ISCF: accelerating detection of disease, and healthy ageing. Biofilms represent a major concern in industry and hospital healthcare settings where they cause severe structural and health problems (Jose Luis Del Pozo 2017; Gibson et al. 1997). Nowadays, the majority of efforts to treat biofilms are based on antimicrobial agents, which has led to one of the biggest issue in the world that is the bacteria and microorganisms antimicrobial resistance (AMR). The decrease of the effectiveness of these agents poses a serious concern and new ways for tackling biofilms without using antimicrobials are needed. The quantification of adhesion and cohesion forces is crucial in understanding, predicting and modelling biofilm development and removal. The primary objective for the placement is to identify the models and characterize the system as a function of the environment, such as pH, Relative Humidity, temperature, ionic strength, and time. We wish to use the opportunity to exchange both personnel and knowledge between academic (UoB) and industrial sectors (P&G). The placement holder will be exposed to a modern cooperate training package such as management skills, technical skills such as bacteria growth and monitoring for chemical industry.
Collaborator Contribution It is anticipated that the outcomes of the planned exchange include the following: 1. New skills: to be transferred between the University of Birmingham to P&G. Maria will use her expertise in microbiology to analyse the presence of biofilms on substrates that are of interest to both industrial and household related applications. In return, Maria will have the opportunity to be trained on a variety of industrial protocols that are not available under an academic research settings. 2. Personal development: Maria will establish in-depth appreciation of industrial perspective on engineering and managing biofilm. Furthermore, she will develop connections with colleagues at P&G, which is tremendously invaluable to an early career researcher. Furthermore, Maria will be offered to attend a number of bespoke training courses by P&G to develop her transferrable skills such as Project Management. 3. New knowledge: concerning the presence and mechanical integrity of biofilms will be generated, which bears fundamental importance to both academic research and industrial impact. This links the past work over different length scales (Goode 2013, Pen 2011), alongside the industrial R&D work. 4. Immediate collaboration: upon success of the placement, whereby key parameters that influence the mechanical properties of biofilm are identified, P&G will commit a further £60k to systematically expand the investigation, as indicated in the supporting letter. This would also strengthen the connection between Maria and colleagues at P&G developed through the placement. 5. Future partnership: University of Birmingham has a strategic partnership with P&G, for the past two decades, with a focus on the Formulation Engineering related work. The proposed work is closely related to the development of new formulated products or processes, and opens new avenues for future collaboration. 6. Societal impact: The learning outcome will help to design new strategies in addressing biofilm related issues in household and healthcare environment. Its impact to our society will be amplified and implemented through large organisation such as P&G who has an established and closed loop in transferring knowledge to real time. We will also engage with other potential partners through the National Biofilm Innovation Centre, to not only disseminate the findings, but to prompt the protocols and techniques developed.
Impact Together with the industrial partners, Dr Masoura identified the skin microbes that are of interest to the particular end applications, and developed the corresponding biofilms on fabrics, which provided invaluable insight to the company in understanding the influence of microbiological activities on the development of fabric care products. A key outcome of the project was to identify the factor(s) that control the adhesion and cohesion of such biofilms - this could be used for the development of new technologies in preventing and removing biofilms from fabrics. Our industrial partner, P&G, was delighted by the initial results generated by Dr Masoura and committed a follow-on fund of £60k to support Maria as a Research Fellow at the University of Birmingham continuing her investigation with a systematic manner. No other support is required from the NBIC for this project. Dr Masoura had subsequently developed her connection with another SME using natural materials for food packaging, and plans to submit a research proposal in the upcoming round of funding call.
Start Year 2020
 
Description NBIC FTMA Placement P_19_2_44 Mechanical properties of biofilm on complex substrate (Zhenyu Zhang) 
Organisation University of Birmingham
Country United Kingdom 
Sector Academic/University 
PI Contribution The aim of this project is to place Maria Masoura, a PhD student with microbiology background, in a world-leading chemical company, Procter & Gamble to: 1. establish appreciation of industrial perspectives of biofilm; 2. develop industrial links with P&G; 3. identify key parameters that manage and engineer biofilms on complex substrates. During the three months placement period, Maria plans to systematically investigate and measure microbial adhesion to model soils and the mechanical properties of the biofilm/soil complex consequently formed. The scientific nature of the proposal is aligned with at least two areas outlined in the ISCF: accelerating detection of disease, and healthy ageing. Biofilms represent a major concern in industry and hospital healthcare settings where they cause severe structural and health problems (Jose Luis Del Pozo 2017; Gibson et al. 1997). Nowadays, the majority of efforts to treat biofilms are based on antimicrobial agents, which has led to one of the biggest issue in the world that is the bacteria and microorganisms antimicrobial resistance (AMR). The decrease of the effectiveness of these agents poses a serious concern and new ways for tackling biofilms without using antimicrobials are needed. The quantification of adhesion and cohesion forces is crucial in understanding, predicting and modelling biofilm development and removal. The primary objective for the placement is to identify the models and characterize the system as a function of the environment, such as pH, Relative Humidity, temperature, ionic strength, and time. We wish to use the opportunity to exchange both personnel and knowledge between academic (UoB) and industrial sectors (P&G). The placement holder will be exposed to a modern cooperate training package such as management skills, technical skills such as bacteria growth and monitoring for chemical industry.
Collaborator Contribution It is anticipated that the outcomes of the planned exchange include the following: 1. New skills: to be transferred between the University of Birmingham to P&G. Maria will use her expertise in microbiology to analyse the presence of biofilms on substrates that are of interest to both industrial and household related applications. In return, Maria will have the opportunity to be trained on a variety of industrial protocols that are not available under an academic research settings. 2. Personal development: Maria will establish in-depth appreciation of industrial perspective on engineering and managing biofilm. Furthermore, she will develop connections with colleagues at P&G, which is tremendously invaluable to an early career researcher. Furthermore, Maria will be offered to attend a number of bespoke training courses by P&G to develop her transferrable skills such as Project Management. 3. New knowledge: concerning the presence and mechanical integrity of biofilms will be generated, which bears fundamental importance to both academic research and industrial impact. This links the past work over different length scales (Goode 2013, Pen 2011), alongside the industrial R&D work. 4. Immediate collaboration: upon success of the placement, whereby key parameters that influence the mechanical properties of biofilm are identified, P&G will commit a further £60k to systematically expand the investigation, as indicated in the supporting letter. This would also strengthen the connection between Maria and colleagues at P&G developed through the placement. 5. Future partnership: University of Birmingham has a strategic partnership with P&G, for the past two decades, with a focus on the Formulation Engineering related work. The proposed work is closely related to the development of new formulated products or processes, and opens new avenues for future collaboration. 6. Societal impact: The learning outcome will help to design new strategies in addressing biofilm related issues in household and healthcare environment. Its impact to our society will be amplified and implemented through large organisation such as P&G who has an established and closed loop in transferring knowledge to real time. We will also engage with other potential partners through the National Biofilm Innovation Centre, to not only disseminate the findings, but to prompt the protocols and techniques developed.
Impact Together with the industrial partners, Dr Masoura identified the skin microbes that are of interest to the particular end applications, and developed the corresponding biofilms on fabrics, which provided invaluable insight to the company in understanding the influence of microbiological activities on the development of fabric care products. A key outcome of the project was to identify the factor(s) that control the adhesion and cohesion of such biofilms - this could be used for the development of new technologies in preventing and removing biofilms from fabrics. Our industrial partner, P&G, was delighted by the initial results generated by Dr Masoura and committed a follow-on fund of £60k to support Maria as a Research Fellow at the University of Birmingham continuing her investigation with a systematic manner. No other support is required from the NBIC for this project. Dr Masoura had subsequently developed her connection with another SME using natural materials for food packaging, and plans to submit a research proposal in the upcoming round of funding call.
Start Year 2020
 
Description NBIC FTMA Placement P_19_2_47 Developing Light Sheet Imaging to Detect and Monitor Biofilm Growth (Campbell Gourlay) 
Organisation CAIRN Research Ltd
Country United Kingdom 
Sector Private 
PI Contribution The overarching aim is to establish light sheet microscopy as a powerful tool that can be used to detect and monitor the formation of biofilms. To date such technology, which can image large surface areas in high resolution rapidly and with low phototoxicity has not been implemented to monitor and detect biofilm growth. The student will receive training in the use of two complementary light-sheet systems produced by Cairn Scientific. Training will take place within their scientific R & D labs in Faversham, Kent, with additional provided by Dr. Laissue (Essex). Both Cairn and Dr. Laissue have significant expertise in the design and implementation of light microscopy solutions. The student will be trained to use two light sheet instruments, an L-SPI for large sample imaging, and a MizarTILT system for high-resolution imaging. Both systems cause very low phototoxicity and can be used for non-invasive, long-term time-lapse fluorescence microscopy. The student will explore their evident potential for the use for detection and monitoring of biofilm growth within industrial settings that are highly relevant to the BBSRC Industry Strategy Challenges a) acceleration of detection of disease and b) precision agriculture. The first aim will be to monitor the development of algal biofilms on plastic tubing used to deliver nutrition within controlled growth precision agricultural facilities. In this case it will be of interest to monitor large areas of tubing at intermediate, cellular resolution to help detect early biofilm formation and to examine the effects of decontamination processes. The second aim will be to examine more complex biofilms at high resolution, revealing subcellular details to help dissect the composition of complex biofilm formation in real time. Such experiments will allow researchers to determine how multi-species biofilms assemble in real time, opening the door to new research-driven approaches to early disease detection and treatment.
Collaborator Contribution The research aims to extend the use of cutting edge microscopy technology into a new field of research that has direct relevance to the BBSRC Industrial Strategy Challenges. Main outputs will arise from the capture of the first comprehensive light sheet microscopy live cell imaging data of algal, bacterial and fungal biofilms developing on industrially relevant materials at high resolution. The data will validate the use of light sheet microscopy for biofilm detection. It will also show that high resolution live cell imaging data can be rapidly acquired over large surface areas, which lends the technique to rapid monitoring of biofilm formation in a variety of automated industrial settings, such as in precision agriculture feeding assemblies that are prone to contamination. The project will lead to the production of protocols that can be used to image biofilms for extended periods of time with limited photo-damage that will contribute to many future publications. We anticipate that the success of the project will lead to further collaborative work between the applicants, Cairn Scientific and a customer base with interests in using rapid cost effective imaging to monitor biofilm contamination or the effectiveness of anti-fouling treatments. Examples of such applications lie within the "smart agriculture" sector where constant monitoring systems play an important role in plant pathogen control or on the surfaces of medical equipment such as IV lines or catheter tubing. A significant output will be the training of the researcher to use cutting edge technology within an industrial R and D environment. The project offers an exciting opportunity for the student to gain experience not only in the development and application of a cutting edge microscopy technique, but in product development and marketing. Experience and results gained from developing new light sheet biofilm detection applications, and results obtained with them, will feed directly into the further collaborative research and research-led teaching done by the investigators at the Universities of Kent and Essex. The instruments and techniques will also be applied within future practical courses run by Dr. Laissue (e.g. NERC course for environmental imaging and joint imaging courses being developed by the Universities of Kent and Essex).
Impact The student gained experience using two light sheet instruments, an L-SPI for large sample imaging, and a MizarTILT system for high-resolution imaging. Use of both systems allowed long term imaging of biofilms and experiments focussed on optomising experimental conditions to reduce photoxicity using mitochondrial fragmentation as a sensitive marker. While the student gained experience working within an industrial setting and in new technology the company also gained new data that can be used to develop new protocols for their instruments. Certain limitations were encountered with the Cairn light sheet instruments that led to a follow on application for an FTMA fellowship within a second imaging company. This application was successful. The company, 3i, has agreed to the student (now a postdoc) to undertake studies within their facility to introduce AI software approaches in combination with their innovative light sheet instruments. It is hoped these experiments will yield unprecedented images of growing fungal biofilms under negligible levels of photoxicity.
Start Year 2020
 
Description NBIC FTMA Placement P_19_2_47 Developing Light Sheet Imaging to Detect and Monitor Biofilm Growth (Campbell Gourlay) 
Organisation National Biofilms Innovation Centre
Sector Private 
PI Contribution The overarching aim is to establish light sheet microscopy as a powerful tool that can be used to detect and monitor the formation of biofilms. To date such technology, which can image large surface areas in high resolution rapidly and with low phototoxicity has not been implemented to monitor and detect biofilm growth. The student will receive training in the use of two complementary light-sheet systems produced by Cairn Scientific. Training will take place within their scientific R & D labs in Faversham, Kent, with additional provided by Dr. Laissue (Essex). Both Cairn and Dr. Laissue have significant expertise in the design and implementation of light microscopy solutions. The student will be trained to use two light sheet instruments, an L-SPI for large sample imaging, and a MizarTILT system for high-resolution imaging. Both systems cause very low phototoxicity and can be used for non-invasive, long-term time-lapse fluorescence microscopy. The student will explore their evident potential for the use for detection and monitoring of biofilm growth within industrial settings that are highly relevant to the BBSRC Industry Strategy Challenges a) acceleration of detection of disease and b) precision agriculture. The first aim will be to monitor the development of algal biofilms on plastic tubing used to deliver nutrition within controlled growth precision agricultural facilities. In this case it will be of interest to monitor large areas of tubing at intermediate, cellular resolution to help detect early biofilm formation and to examine the effects of decontamination processes. The second aim will be to examine more complex biofilms at high resolution, revealing subcellular details to help dissect the composition of complex biofilm formation in real time. Such experiments will allow researchers to determine how multi-species biofilms assemble in real time, opening the door to new research-driven approaches to early disease detection and treatment.
Collaborator Contribution The research aims to extend the use of cutting edge microscopy technology into a new field of research that has direct relevance to the BBSRC Industrial Strategy Challenges. Main outputs will arise from the capture of the first comprehensive light sheet microscopy live cell imaging data of algal, bacterial and fungal biofilms developing on industrially relevant materials at high resolution. The data will validate the use of light sheet microscopy for biofilm detection. It will also show that high resolution live cell imaging data can be rapidly acquired over large surface areas, which lends the technique to rapid monitoring of biofilm formation in a variety of automated industrial settings, such as in precision agriculture feeding assemblies that are prone to contamination. The project will lead to the production of protocols that can be used to image biofilms for extended periods of time with limited photo-damage that will contribute to many future publications. We anticipate that the success of the project will lead to further collaborative work between the applicants, Cairn Scientific and a customer base with interests in using rapid cost effective imaging to monitor biofilm contamination or the effectiveness of anti-fouling treatments. Examples of such applications lie within the "smart agriculture" sector where constant monitoring systems play an important role in plant pathogen control or on the surfaces of medical equipment such as IV lines or catheter tubing. A significant output will be the training of the researcher to use cutting edge technology within an industrial R and D environment. The project offers an exciting opportunity for the student to gain experience not only in the development and application of a cutting edge microscopy technique, but in product development and marketing. Experience and results gained from developing new light sheet biofilm detection applications, and results obtained with them, will feed directly into the further collaborative research and research-led teaching done by the investigators at the Universities of Kent and Essex. The instruments and techniques will also be applied within future practical courses run by Dr. Laissue (e.g. NERC course for environmental imaging and joint imaging courses being developed by the Universities of Kent and Essex).
Impact The student gained experience using two light sheet instruments, an L-SPI for large sample imaging, and a MizarTILT system for high-resolution imaging. Use of both systems allowed long term imaging of biofilms and experiments focussed on optomising experimental conditions to reduce photoxicity using mitochondrial fragmentation as a sensitive marker. While the student gained experience working within an industrial setting and in new technology the company also gained new data that can be used to develop new protocols for their instruments. Certain limitations were encountered with the Cairn light sheet instruments that led to a follow on application for an FTMA fellowship within a second imaging company. This application was successful. The company, 3i, has agreed to the student (now a postdoc) to undertake studies within their facility to introduce AI software approaches in combination with their innovative light sheet instruments. It is hoped these experiments will yield unprecedented images of growing fungal biofilms under negligible levels of photoxicity.
Start Year 2020
 
Description NBIC FTMA Placement P_19_2_47 Developing Light Sheet Imaging to Detect and Monitor Biofilm Growth (Campbell Gourlay) 
Organisation University of Kent
Country United Kingdom 
Sector Academic/University 
PI Contribution The overarching aim is to establish light sheet microscopy as a powerful tool that can be used to detect and monitor the formation of biofilms. To date such technology, which can image large surface areas in high resolution rapidly and with low phototoxicity has not been implemented to monitor and detect biofilm growth. The student will receive training in the use of two complementary light-sheet systems produced by Cairn Scientific. Training will take place within their scientific R & D labs in Faversham, Kent, with additional provided by Dr. Laissue (Essex). Both Cairn and Dr. Laissue have significant expertise in the design and implementation of light microscopy solutions. The student will be trained to use two light sheet instruments, an L-SPI for large sample imaging, and a MizarTILT system for high-resolution imaging. Both systems cause very low phototoxicity and can be used for non-invasive, long-term time-lapse fluorescence microscopy. The student will explore their evident potential for the use for detection and monitoring of biofilm growth within industrial settings that are highly relevant to the BBSRC Industry Strategy Challenges a) acceleration of detection of disease and b) precision agriculture. The first aim will be to monitor the development of algal biofilms on plastic tubing used to deliver nutrition within controlled growth precision agricultural facilities. In this case it will be of interest to monitor large areas of tubing at intermediate, cellular resolution to help detect early biofilm formation and to examine the effects of decontamination processes. The second aim will be to examine more complex biofilms at high resolution, revealing subcellular details to help dissect the composition of complex biofilm formation in real time. Such experiments will allow researchers to determine how multi-species biofilms assemble in real time, opening the door to new research-driven approaches to early disease detection and treatment.
Collaborator Contribution The research aims to extend the use of cutting edge microscopy technology into a new field of research that has direct relevance to the BBSRC Industrial Strategy Challenges. Main outputs will arise from the capture of the first comprehensive light sheet microscopy live cell imaging data of algal, bacterial and fungal biofilms developing on industrially relevant materials at high resolution. The data will validate the use of light sheet microscopy for biofilm detection. It will also show that high resolution live cell imaging data can be rapidly acquired over large surface areas, which lends the technique to rapid monitoring of biofilm formation in a variety of automated industrial settings, such as in precision agriculture feeding assemblies that are prone to contamination. The project will lead to the production of protocols that can be used to image biofilms for extended periods of time with limited photo-damage that will contribute to many future publications. We anticipate that the success of the project will lead to further collaborative work between the applicants, Cairn Scientific and a customer base with interests in using rapid cost effective imaging to monitor biofilm contamination or the effectiveness of anti-fouling treatments. Examples of such applications lie within the "smart agriculture" sector where constant monitoring systems play an important role in plant pathogen control or on the surfaces of medical equipment such as IV lines or catheter tubing. A significant output will be the training of the researcher to use cutting edge technology within an industrial R and D environment. The project offers an exciting opportunity for the student to gain experience not only in the development and application of a cutting edge microscopy technique, but in product development and marketing. Experience and results gained from developing new light sheet biofilm detection applications, and results obtained with them, will feed directly into the further collaborative research and research-led teaching done by the investigators at the Universities of Kent and Essex. The instruments and techniques will also be applied within future practical courses run by Dr. Laissue (e.g. NERC course for environmental imaging and joint imaging courses being developed by the Universities of Kent and Essex).
Impact The student gained experience using two light sheet instruments, an L-SPI for large sample imaging, and a MizarTILT system for high-resolution imaging. Use of both systems allowed long term imaging of biofilms and experiments focussed on optomising experimental conditions to reduce photoxicity using mitochondrial fragmentation as a sensitive marker. While the student gained experience working within an industrial setting and in new technology the company also gained new data that can be used to develop new protocols for their instruments. Certain limitations were encountered with the Cairn light sheet instruments that led to a follow on application for an FTMA fellowship within a second imaging company. This application was successful. The company, 3i, has agreed to the student (now a postdoc) to undertake studies within their facility to introduce AI software approaches in combination with their innovative light sheet instruments. It is hoped these experiments will yield unprecedented images of growing fungal biofilms under negligible levels of photoxicity.
Start Year 2020
 
Description NBIC FTMA Placements P_19_01 and P_19_01_2 16S Metagenomics product development (Sandra Wilks) 
Organisation National Biofilms Innovation Centre
Sector Private 
PI Contribution I will be spending 3 months working at YouSeq Ltd. YouSeq is a new company that develops Next Generation Sequencing (NGS) kits. YouSeq was founded by the team behind PrimerDesign Ltd (a successful qPCR product business spun out of the University of Southampton), that I have purchased product from and was previously successful in winning a sponsorship award from. Youseq have a new 16S metagenomics product with the power to identify (to a species level) complex bacteria populations from environmental and clinical samples. YouSeq are also developing a novel sampling tool for quick and easy sampling and simultaneous DNA extraction from environmental bacteria (including biofilms). I will be working to develop this new product further through a programme of laboratory work, field testing and market research. I will use my existing knowledge of 16S bacterial metagenomics alongside my in depth understanding of biofilm formation to help advise the team in the development of the product further. I will test the product extensively with real world samples to help validate the product and identify areas for further improvement. I will also spend time talking to clinicians and researchers to understand in more detail the challenges and pain points that they face in order to understand best how to develop the product for maximum societal impact. The secondment will allow me to experience the fast-paced commercial start-up environment and apply my knowledge in a tangible and very direct manner. I will have the opportunity to learn new commercial skills in product development, market research and commercialisation of scientific knowledge. All of which, I believe, offers me a rich and exciting opportunity to experience the commercial world of Science at this pivotal moment in my personal and professional development.
Collaborator Contribution The 16S Metagenomics kit that I will be working to develop will have enormous societal and economic benefit. Microbial infection of environmental surfaces in hospitals has a massive impact on patient outcome and is known to be a reservoir for clinical infection. Accurate, cost effect, routine identification of complex bacteria populations from environmental samples will be a powerful tool in the fight against hospital acquired infection. As well as improved patient outcomes, this has the potential to provide enormous cost savings by preventing infection before it occurs. The key measurable outcome from my visit will be the commercial launch of the 16S metagenomics product of which I will be an integral part of the launch team. The product is in beta testing and requires some final development and a body of scientific and market research data behind it. I will produce/collate this data in to a final report. This data will be used to make any final improvements to the product ahead of launch. I hope to be able to use my own network of clinical contacts at Southampton General Hospital to perform a small-scale collaboration/testing programme during the secondment. I will also write a "white paper" centred around the use of the 16S Metagenomics kit for marketing and communication purposes. My current academic department also have a requirement for more 16S metagenomic testing in future so there is potential for further collaboration between YouSeq and the University of Southampton beyond the duration of my secondment. Further developments: After completing his project with YouSeq in early January 2019, Dr Winnard successfully found employment with Novagene, a global NGS service provider. YouSeq are currently undertaking a marketing drive for the product with great hopes for significant commercial success that will bring further income and employment to the local community.
Impact The outcome has been a successful product launch. Chris has been successful in taking the prototype product, identifying final issues and implement improvements to make the kit robust for commercial use. He has performed detailed studies on sensitivity and specificity and produced good quality data to satisfy the company's quality standards as well as for use in marketing purposes. Chris has also been involved in various marketing related tasks associated with product launch including developing the product instructions-for-use further as well as website and printed marketing content. Chris has also been involved in the beta version of product launch with trials of the kit occurring in the US, South Africa, Portugal and the UK. Chris also took part in commercial and technical discussions with the company's first significant customer for the product signing an agreement with YouSeq this week.
Start Year 2019
 
Description NBIC FTMA Placements P_19_01 and P_19_01_2 16S Metagenomics product development (Sandra Wilks) 
Organisation University of Southampton
Country United Kingdom 
Sector Academic/University 
PI Contribution I will be spending 3 months working at YouSeq Ltd. YouSeq is a new company that develops Next Generation Sequencing (NGS) kits. YouSeq was founded by the team behind PrimerDesign Ltd (a successful qPCR product business spun out of the University of Southampton), that I have purchased product from and was previously successful in winning a sponsorship award from. Youseq have a new 16S metagenomics product with the power to identify (to a species level) complex bacteria populations from environmental and clinical samples. YouSeq are also developing a novel sampling tool for quick and easy sampling and simultaneous DNA extraction from environmental bacteria (including biofilms). I will be working to develop this new product further through a programme of laboratory work, field testing and market research. I will use my existing knowledge of 16S bacterial metagenomics alongside my in depth understanding of biofilm formation to help advise the team in the development of the product further. I will test the product extensively with real world samples to help validate the product and identify areas for further improvement. I will also spend time talking to clinicians and researchers to understand in more detail the challenges and pain points that they face in order to understand best how to develop the product for maximum societal impact. The secondment will allow me to experience the fast-paced commercial start-up environment and apply my knowledge in a tangible and very direct manner. I will have the opportunity to learn new commercial skills in product development, market research and commercialisation of scientific knowledge. All of which, I believe, offers me a rich and exciting opportunity to experience the commercial world of Science at this pivotal moment in my personal and professional development.
Collaborator Contribution The 16S Metagenomics kit that I will be working to develop will have enormous societal and economic benefit. Microbial infection of environmental surfaces in hospitals has a massive impact on patient outcome and is known to be a reservoir for clinical infection. Accurate, cost effect, routine identification of complex bacteria populations from environmental samples will be a powerful tool in the fight against hospital acquired infection. As well as improved patient outcomes, this has the potential to provide enormous cost savings by preventing infection before it occurs. The key measurable outcome from my visit will be the commercial launch of the 16S metagenomics product of which I will be an integral part of the launch team. The product is in beta testing and requires some final development and a body of scientific and market research data behind it. I will produce/collate this data in to a final report. This data will be used to make any final improvements to the product ahead of launch. I hope to be able to use my own network of clinical contacts at Southampton General Hospital to perform a small-scale collaboration/testing programme during the secondment. I will also write a "white paper" centred around the use of the 16S Metagenomics kit for marketing and communication purposes. My current academic department also have a requirement for more 16S metagenomic testing in future so there is potential for further collaboration between YouSeq and the University of Southampton beyond the duration of my secondment. Further developments: After completing his project with YouSeq in early January 2019, Dr Winnard successfully found employment with Novagene, a global NGS service provider. YouSeq are currently undertaking a marketing drive for the product with great hopes for significant commercial success that will bring further income and employment to the local community.
Impact The outcome has been a successful product launch. Chris has been successful in taking the prototype product, identifying final issues and implement improvements to make the kit robust for commercial use. He has performed detailed studies on sensitivity and specificity and produced good quality data to satisfy the company's quality standards as well as for use in marketing purposes. Chris has also been involved in various marketing related tasks associated with product launch including developing the product instructions-for-use further as well as website and printed marketing content. Chris has also been involved in the beta version of product launch with trials of the kit occurring in the US, South Africa, Portugal and the UK. Chris also took part in commercial and technical discussions with the company's first significant customer for the product signing an agreement with YouSeq this week.
Start Year 2019
 
Description NBIC FTMA Placements P_19_01 and P_19_01_2 16S Metagenomics product development (Sandra Wilks) 
Organisation YouSeq Ltd
Country United Kingdom 
Sector Private 
PI Contribution I will be spending 3 months working at YouSeq Ltd. YouSeq is a new company that develops Next Generation Sequencing (NGS) kits. YouSeq was founded by the team behind PrimerDesign Ltd (a successful qPCR product business spun out of the University of Southampton), that I have purchased product from and was previously successful in winning a sponsorship award from. Youseq have a new 16S metagenomics product with the power to identify (to a species level) complex bacteria populations from environmental and clinical samples. YouSeq are also developing a novel sampling tool for quick and easy sampling and simultaneous DNA extraction from environmental bacteria (including biofilms). I will be working to develop this new product further through a programme of laboratory work, field testing and market research. I will use my existing knowledge of 16S bacterial metagenomics alongside my in depth understanding of biofilm formation to help advise the team in the development of the product further. I will test the product extensively with real world samples to help validate the product and identify areas for further improvement. I will also spend time talking to clinicians and researchers to understand in more detail the challenges and pain points that they face in order to understand best how to develop the product for maximum societal impact. The secondment will allow me to experience the fast-paced commercial start-up environment and apply my knowledge in a tangible and very direct manner. I will have the opportunity to learn new commercial skills in product development, market research and commercialisation of scientific knowledge. All of which, I believe, offers me a rich and exciting opportunity to experience the commercial world of Science at this pivotal moment in my personal and professional development.
Collaborator Contribution The 16S Metagenomics kit that I will be working to develop will have enormous societal and economic benefit. Microbial infection of environmental surfaces in hospitals has a massive impact on patient outcome and is known to be a reservoir for clinical infection. Accurate, cost effect, routine identification of complex bacteria populations from environmental samples will be a powerful tool in the fight against hospital acquired infection. As well as improved patient outcomes, this has the potential to provide enormous cost savings by preventing infection before it occurs. The key measurable outcome from my visit will be the commercial launch of the 16S metagenomics product of which I will be an integral part of the launch team. The product is in beta testing and requires some final development and a body of scientific and market research data behind it. I will produce/collate this data in to a final report. This data will be used to make any final improvements to the product ahead of launch. I hope to be able to use my own network of clinical contacts at Southampton General Hospital to perform a small-scale collaboration/testing programme during the secondment. I will also write a "white paper" centred around the use of the 16S Metagenomics kit for marketing and communication purposes. My current academic department also have a requirement for more 16S metagenomic testing in future so there is potential for further collaboration between YouSeq and the University of Southampton beyond the duration of my secondment. Further developments: After completing his project with YouSeq in early January 2019, Dr Winnard successfully found employment with Novagene, a global NGS service provider. YouSeq are currently undertaking a marketing drive for the product with great hopes for significant commercial success that will bring further income and employment to the local community.
Impact The outcome has been a successful product launch. Chris has been successful in taking the prototype product, identifying final issues and implement improvements to make the kit robust for commercial use. He has performed detailed studies on sensitivity and specificity and produced good quality data to satisfy the company's quality standards as well as for use in marketing purposes. Chris has also been involved in various marketing related tasks associated with product launch including developing the product instructions-for-use further as well as website and printed marketing content. Chris has also been involved in the beta version of product launch with trials of the kit occurring in the US, South Africa, Portugal and the UK. Chris also took part in commercial and technical discussions with the company's first significant customer for the product signing an agreement with YouSeq this week.
Start Year 2019
 
Description National Biofilm Innovation Centre 
Organisation Aberystwyth University
Country United Kingdom 
Sector Academic/University 
PI Contribution 1) Sectorial workshops with NBIC Company / Academic Community. This gives the opportunity to form links and possible POC projects to be identified. 2) Holding Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. This gives the opportunity to influence NBIC strategy and funding calls. 3) Identifying NBIC fully or part funded Strategic Research (Defined by Sectorial Workshops and approved by Boards). 4) Providing access to POC calls aligned with NBIC Strategy along with Industrial Partner via Sector or Theme. Competitive transparent Open Process Univ signed up to NBIC with companies willing to sign Project Agreement. Selected using range of criteria within a template case. Support in POC submissions and advice from NBIC engagement officers 5) Facilitating collaborative Access to facilities (eg Diamond / Hartree) (Note Science and Industrial Advisory Boards will have regular review and input into these processes and content of themes etc)
Collaborator Contribution 1) Participation in Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. 2) Application to the NBIC Proof of Concept funding for translational research projects 3) Application to studentships and placements with industrial partners
Impact NBIC has awarded 65 Proof of Concept projects, these projects are with the academic partners lists and industry collaborators. We have held three workshop (Biofilm detection, engineering and management), also see publications. The National Biofilms innovation centre is working across disciplines, including: engineering, chemistry, surface and interface science, functional materials, soft matter and non-equilibrium physics, environmental sciences, microbiology, 'omics, bioinformatics, computation and advanced techniques.
Start Year 2018
 
Description National Biofilm Innovation Centre 
Organisation Aston University
Country United Kingdom 
Sector Academic/University 
PI Contribution 1) Sectorial workshops with NBIC Company / Academic Community. This gives the opportunity to form links and possible POC projects to be identified. 2) Holding Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. This gives the opportunity to influence NBIC strategy and funding calls. 3) Identifying NBIC fully or part funded Strategic Research (Defined by Sectorial Workshops and approved by Boards). 4) Providing access to POC calls aligned with NBIC Strategy along with Industrial Partner via Sector or Theme. Competitive transparent Open Process Univ signed up to NBIC with companies willing to sign Project Agreement. Selected using range of criteria within a template case. Support in POC submissions and advice from NBIC engagement officers 5) Facilitating collaborative Access to facilities (eg Diamond / Hartree) (Note Science and Industrial Advisory Boards will have regular review and input into these processes and content of themes etc)
Collaborator Contribution 1) Participation in Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. 2) Application to the NBIC Proof of Concept funding for translational research projects 3) Application to studentships and placements with industrial partners
Impact NBIC has awarded 65 Proof of Concept projects, these projects are with the academic partners lists and industry collaborators. We have held three workshop (Biofilm detection, engineering and management), also see publications. The National Biofilms innovation centre is working across disciplines, including: engineering, chemistry, surface and interface science, functional materials, soft matter and non-equilibrium physics, environmental sciences, microbiology, 'omics, bioinformatics, computation and advanced techniques.
Start Year 2018
 
Description National Biofilm Innovation Centre 
Organisation Barts Health NHS Trust
Country United Kingdom 
Sector Public 
PI Contribution 1) Sectorial workshops with NBIC Company / Academic Community. This gives the opportunity to form links and possible POC projects to be identified. 2) Holding Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. This gives the opportunity to influence NBIC strategy and funding calls. 3) Identifying NBIC fully or part funded Strategic Research (Defined by Sectorial Workshops and approved by Boards). 4) Providing access to POC calls aligned with NBIC Strategy along with Industrial Partner via Sector or Theme. Competitive transparent Open Process Univ signed up to NBIC with companies willing to sign Project Agreement. Selected using range of criteria within a template case. Support in POC submissions and advice from NBIC engagement officers 5) Facilitating collaborative Access to facilities (eg Diamond / Hartree) (Note Science and Industrial Advisory Boards will have regular review and input into these processes and content of themes etc)
Collaborator Contribution 1) Participation in Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. 2) Application to the NBIC Proof of Concept funding for translational research projects 3) Application to studentships and placements with industrial partners
Impact NBIC has awarded 65 Proof of Concept projects, these projects are with the academic partners lists and industry collaborators. We have held three workshop (Biofilm detection, engineering and management), also see publications. The National Biofilms innovation centre is working across disciplines, including: engineering, chemistry, surface and interface science, functional materials, soft matter and non-equilibrium physics, environmental sciences, microbiology, 'omics, bioinformatics, computation and advanced techniques.
Start Year 2018
 
Description National Biofilm Innovation Centre 
Organisation British Geological Survey
Country United Kingdom 
Sector Academic/University 
PI Contribution 1) Sectorial workshops with NBIC Company / Academic Community. This gives the opportunity to form links and possible POC projects to be identified. 2) Holding Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. This gives the opportunity to influence NBIC strategy and funding calls. 3) Identifying NBIC fully or part funded Strategic Research (Defined by Sectorial Workshops and approved by Boards). 4) Providing access to POC calls aligned with NBIC Strategy along with Industrial Partner via Sector or Theme. Competitive transparent Open Process Univ signed up to NBIC with companies willing to sign Project Agreement. Selected using range of criteria within a template case. Support in POC submissions and advice from NBIC engagement officers 5) Facilitating collaborative Access to facilities (eg Diamond / Hartree) (Note Science and Industrial Advisory Boards will have regular review and input into these processes and content of themes etc)
Collaborator Contribution 1) Participation in Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. 2) Application to the NBIC Proof of Concept funding for translational research projects 3) Application to studentships and placements with industrial partners
Impact NBIC has awarded 65 Proof of Concept projects, these projects are with the academic partners lists and industry collaborators. We have held three workshop (Biofilm detection, engineering and management), also see publications. The National Biofilms innovation centre is working across disciplines, including: engineering, chemistry, surface and interface science, functional materials, soft matter and non-equilibrium physics, environmental sciences, microbiology, 'omics, bioinformatics, computation and advanced techniques.
Start Year 2018
 
Description National Biofilm Innovation Centre 
Organisation Brunel University London
Country United Kingdom 
Sector Academic/University 
PI Contribution 1) Sectorial workshops with NBIC Company / Academic Community. This gives the opportunity to form links and possible POC projects to be identified. 2) Holding Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. This gives the opportunity to influence NBIC strategy and funding calls. 3) Identifying NBIC fully or part funded Strategic Research (Defined by Sectorial Workshops and approved by Boards). 4) Providing access to POC calls aligned with NBIC Strategy along with Industrial Partner via Sector or Theme. Competitive transparent Open Process Univ signed up to NBIC with companies willing to sign Project Agreement. Selected using range of criteria within a template case. Support in POC submissions and advice from NBIC engagement officers 5) Facilitating collaborative Access to facilities (eg Diamond / Hartree) (Note Science and Industrial Advisory Boards will have regular review and input into these processes and content of themes etc)
Collaborator Contribution 1) Participation in Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. 2) Application to the NBIC Proof of Concept funding for translational research projects 3) Application to studentships and placements with industrial partners
Impact NBIC has awarded 65 Proof of Concept projects, these projects are with the academic partners lists and industry collaborators. We have held three workshop (Biofilm detection, engineering and management), also see publications. The National Biofilms innovation centre is working across disciplines, including: engineering, chemistry, surface and interface science, functional materials, soft matter and non-equilibrium physics, environmental sciences, microbiology, 'omics, bioinformatics, computation and advanced techniques.
Start Year 2018
 
Description National Biofilm Innovation Centre 
Organisation Canterbury Christ Church University
Country United Kingdom 
Sector Academic/University 
PI Contribution 1) Sectorial workshops with NBIC Company / Academic Community. This gives the opportunity to form links and possible POC projects to be identified. 2) Holding Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. This gives the opportunity to influence NBIC strategy and funding calls. 3) Identifying NBIC fully or part funded Strategic Research (Defined by Sectorial Workshops and approved by Boards). 4) Providing access to POC calls aligned with NBIC Strategy along with Industrial Partner via Sector or Theme. Competitive transparent Open Process Univ signed up to NBIC with companies willing to sign Project Agreement. Selected using range of criteria within a template case. Support in POC submissions and advice from NBIC engagement officers 5) Facilitating collaborative Access to facilities (eg Diamond / Hartree) (Note Science and Industrial Advisory Boards will have regular review and input into these processes and content of themes etc)
Collaborator Contribution 1) Participation in Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. 2) Application to the NBIC Proof of Concept funding for translational research projects 3) Application to studentships and placements with industrial partners
Impact NBIC has awarded 65 Proof of Concept projects, these projects are with the academic partners lists and industry collaborators. We have held three workshop (Biofilm detection, engineering and management), also see publications. The National Biofilms innovation centre is working across disciplines, including: engineering, chemistry, surface and interface science, functional materials, soft matter and non-equilibrium physics, environmental sciences, microbiology, 'omics, bioinformatics, computation and advanced techniques.
Start Year 2018
 
Description National Biofilm Innovation Centre 
Organisation Cardiff University
Country United Kingdom 
Sector Academic/University 
PI Contribution 1) Sectorial workshops with NBIC Company / Academic Community. This gives the opportunity to form links and possible POC projects to be identified. 2) Holding Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. This gives the opportunity to influence NBIC strategy and funding calls. 3) Identifying NBIC fully or part funded Strategic Research (Defined by Sectorial Workshops and approved by Boards). 4) Providing access to POC calls aligned with NBIC Strategy along with Industrial Partner via Sector or Theme. Competitive transparent Open Process Univ signed up to NBIC with companies willing to sign Project Agreement. Selected using range of criteria within a template case. Support in POC submissions and advice from NBIC engagement officers 5) Facilitating collaborative Access to facilities (eg Diamond / Hartree) (Note Science and Industrial Advisory Boards will have regular review and input into these processes and content of themes etc)
Collaborator Contribution 1) Participation in Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. 2) Application to the NBIC Proof of Concept funding for translational research projects 3) Application to studentships and placements with industrial partners
Impact NBIC has awarded 65 Proof of Concept projects, these projects are with the academic partners lists and industry collaborators. We have held three workshop (Biofilm detection, engineering and management), also see publications. The National Biofilms innovation centre is working across disciplines, including: engineering, chemistry, surface and interface science, functional materials, soft matter and non-equilibrium physics, environmental sciences, microbiology, 'omics, bioinformatics, computation and advanced techniques.
Start Year 2018
 
Description National Biofilm Innovation Centre 
Organisation Cranfield University
Country United Kingdom 
Sector Academic/University 
PI Contribution 1) Sectorial workshops with NBIC Company / Academic Community. This gives the opportunity to form links and possible POC projects to be identified. 2) Holding Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. This gives the opportunity to influence NBIC strategy and funding calls. 3) Identifying NBIC fully or part funded Strategic Research (Defined by Sectorial Workshops and approved by Boards). 4) Providing access to POC calls aligned with NBIC Strategy along with Industrial Partner via Sector or Theme. Competitive transparent Open Process Univ signed up to NBIC with companies willing to sign Project Agreement. Selected using range of criteria within a template case. Support in POC submissions and advice from NBIC engagement officers 5) Facilitating collaborative Access to facilities (eg Diamond / Hartree) (Note Science and Industrial Advisory Boards will have regular review and input into these processes and content of themes etc)
Collaborator Contribution 1) Participation in Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. 2) Application to the NBIC Proof of Concept funding for translational research projects 3) Application to studentships and placements with industrial partners
Impact NBIC has awarded 65 Proof of Concept projects, these projects are with the academic partners lists and industry collaborators. We have held three workshop (Biofilm detection, engineering and management), also see publications. The National Biofilms innovation centre is working across disciplines, including: engineering, chemistry, surface and interface science, functional materials, soft matter and non-equilibrium physics, environmental sciences, microbiology, 'omics, bioinformatics, computation and advanced techniques.
Start Year 2018
 
Description National Biofilm Innovation Centre 
Organisation De Montfort University
Country United Kingdom 
Sector Academic/University 
PI Contribution 1) Sectorial workshops with NBIC Company / Academic Community. This gives the opportunity to form links and possible POC projects to be identified. 2) Holding Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. This gives the opportunity to influence NBIC strategy and funding calls. 3) Identifying NBIC fully or part funded Strategic Research (Defined by Sectorial Workshops and approved by Boards). 4) Providing access to POC calls aligned with NBIC Strategy along with Industrial Partner via Sector or Theme. Competitive transparent Open Process Univ signed up to NBIC with companies willing to sign Project Agreement. Selected using range of criteria within a template case. Support in POC submissions and advice from NBIC engagement officers 5) Facilitating collaborative Access to facilities (eg Diamond / Hartree) (Note Science and Industrial Advisory Boards will have regular review and input into these processes and content of themes etc)
Collaborator Contribution 1) Participation in Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. 2) Application to the NBIC Proof of Concept funding for translational research projects 3) Application to studentships and placements with industrial partners
Impact NBIC has awarded 65 Proof of Concept projects, these projects are with the academic partners lists and industry collaborators. We have held three workshop (Biofilm detection, engineering and management), also see publications. The National Biofilms innovation centre is working across disciplines, including: engineering, chemistry, surface and interface science, functional materials, soft matter and non-equilibrium physics, environmental sciences, microbiology, 'omics, bioinformatics, computation and advanced techniques.
Start Year 2018
 
Description National Biofilm Innovation Centre 
Organisation Durham University
Country United Kingdom 
Sector Academic/University 
PI Contribution 1) Sectorial workshops with NBIC Company / Academic Community. This gives the opportunity to form links and possible POC projects to be identified. 2) Holding Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. This gives the opportunity to influence NBIC strategy and funding calls. 3) Identifying NBIC fully or part funded Strategic Research (Defined by Sectorial Workshops and approved by Boards). 4) Providing access to POC calls aligned with NBIC Strategy along with Industrial Partner via Sector or Theme. Competitive transparent Open Process Univ signed up to NBIC with companies willing to sign Project Agreement. Selected using range of criteria within a template case. Support in POC submissions and advice from NBIC engagement officers 5) Facilitating collaborative Access to facilities (eg Diamond / Hartree) (Note Science and Industrial Advisory Boards will have regular review and input into these processes and content of themes etc)
Collaborator Contribution 1) Participation in Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. 2) Application to the NBIC Proof of Concept funding for translational research projects 3) Application to studentships and placements with industrial partners
Impact NBIC has awarded 65 Proof of Concept projects, these projects are with the academic partners lists and industry collaborators. We have held three workshop (Biofilm detection, engineering and management), also see publications. The National Biofilms innovation centre is working across disciplines, including: engineering, chemistry, surface and interface science, functional materials, soft matter and non-equilibrium physics, environmental sciences, microbiology, 'omics, bioinformatics, computation and advanced techniques.
Start Year 2018
 
Description National Biofilm Innovation Centre 
Organisation Earlham Institute
Country United Kingdom 
Sector Academic/University 
PI Contribution 1) Sectorial workshops with NBIC Company / Academic Community. This gives the opportunity to form links and possible POC projects to be identified. 2) Holding Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. This gives the opportunity to influence NBIC strategy and funding calls. 3) Identifying NBIC fully or part funded Strategic Research (Defined by Sectorial Workshops and approved by Boards). 4) Providing access to POC calls aligned with NBIC Strategy along with Industrial Partner via Sector or Theme. Competitive transparent Open Process Univ signed up to NBIC with companies willing to sign Project Agreement. Selected using range of criteria within a template case. Support in POC submissions and advice from NBIC engagement officers 5) Facilitating collaborative Access to facilities (eg Diamond / Hartree) (Note Science and Industrial Advisory Boards will have regular review and input into these processes and content of themes etc)
Collaborator Contribution 1) Participation in Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. 2) Application to the NBIC Proof of Concept funding for translational research projects 3) Application to studentships and placements with industrial partners
Impact NBIC has awarded 65 Proof of Concept projects, these projects are with the academic partners lists and industry collaborators. We have held three workshop (Biofilm detection, engineering and management), also see publications. The National Biofilms innovation centre is working across disciplines, including: engineering, chemistry, surface and interface science, functional materials, soft matter and non-equilibrium physics, environmental sciences, microbiology, 'omics, bioinformatics, computation and advanced techniques.
Start Year 2018
 
Description National Biofilm Innovation Centre 
Organisation Edinburgh Napier University
Country United Kingdom 
Sector Academic/University 
PI Contribution 1) Sectorial workshops with NBIC Company / Academic Community. This gives the opportunity to form links and possible POC projects to be identified. 2) Holding Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. This gives the opportunity to influence NBIC strategy and funding calls. 3) Identifying NBIC fully or part funded Strategic Research (Defined by Sectorial Workshops and approved by Boards). 4) Providing access to POC calls aligned with NBIC Strategy along with Industrial Partner via Sector or Theme. Competitive transparent Open Process Univ signed up to NBIC with companies willing to sign Project Agreement. Selected using range of criteria within a template case. Support in POC submissions and advice from NBIC engagement officers 5) Facilitating collaborative Access to facilities (eg Diamond / Hartree) (Note Science and Industrial Advisory Boards will have regular review and input into these processes and content of themes etc)
Collaborator Contribution 1) Participation in Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. 2) Application to the NBIC Proof of Concept funding for translational research projects 3) Application to studentships and placements with industrial partners
Impact NBIC has awarded 65 Proof of Concept projects, these projects are with the academic partners lists and industry collaborators. We have held three workshop (Biofilm detection, engineering and management), also see publications. The National Biofilms innovation centre is working across disciplines, including: engineering, chemistry, surface and interface science, functional materials, soft matter and non-equilibrium physics, environmental sciences, microbiology, 'omics, bioinformatics, computation and advanced techniques.
Start Year 2018
 
Description National Biofilm Innovation Centre 
Organisation Glasgow Caledonian University
Country United Kingdom 
Sector Academic/University 
PI Contribution 1) Sectorial workshops with NBIC Company / Academic Community. This gives the opportunity to form links and possible POC projects to be identified. 2) Holding Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. This gives the opportunity to influence NBIC strategy and funding calls. 3) Identifying NBIC fully or part funded Strategic Research (Defined by Sectorial Workshops and approved by Boards). 4) Providing access to POC calls aligned with NBIC Strategy along with Industrial Partner via Sector or Theme. Competitive transparent Open Process Univ signed up to NBIC with companies willing to sign Project Agreement. Selected using range of criteria within a template case. Support in POC submissions and advice from NBIC engagement officers 5) Facilitating collaborative Access to facilities (eg Diamond / Hartree) (Note Science and Industrial Advisory Boards will have regular review and input into these processes and content of themes etc)
Collaborator Contribution 1) Participation in Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. 2) Application to the NBIC Proof of Concept funding for translational research projects 3) Application to studentships and placements with industrial partners
Impact NBIC has awarded 65 Proof of Concept projects, these projects are with the academic partners lists and industry collaborators. We have held three workshop (Biofilm detection, engineering and management), also see publications. The National Biofilms innovation centre is working across disciplines, including: engineering, chemistry, surface and interface science, functional materials, soft matter and non-equilibrium physics, environmental sciences, microbiology, 'omics, bioinformatics, computation and advanced techniques.
Start Year 2018
 
Description National Biofilm Innovation Centre 
Organisation Heriot-Watt University
Country United Kingdom 
Sector Academic/University 
PI Contribution 1) Sectorial workshops with NBIC Company / Academic Community. This gives the opportunity to form links and possible POC projects to be identified. 2) Holding Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. This gives the opportunity to influence NBIC strategy and funding calls. 3) Identifying NBIC fully or part funded Strategic Research (Defined by Sectorial Workshops and approved by Boards). 4) Providing access to POC calls aligned with NBIC Strategy along with Industrial Partner via Sector or Theme. Competitive transparent Open Process Univ signed up to NBIC with companies willing to sign Project Agreement. Selected using range of criteria within a template case. Support in POC submissions and advice from NBIC engagement officers 5) Facilitating collaborative Access to facilities (eg Diamond / Hartree) (Note Science and Industrial Advisory Boards will have regular review and input into these processes and content of themes etc)
Collaborator Contribution 1) Participation in Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. 2) Application to the NBIC Proof of Concept funding for translational research projects 3) Application to studentships and placements with industrial partners
Impact NBIC has awarded 65 Proof of Concept projects, these projects are with the academic partners lists and industry collaborators. We have held three workshop (Biofilm detection, engineering and management), also see publications. The National Biofilms innovation centre is working across disciplines, including: engineering, chemistry, surface and interface science, functional materials, soft matter and non-equilibrium physics, environmental sciences, microbiology, 'omics, bioinformatics, computation and advanced techniques.
Start Year 2018
 
Description National Biofilm Innovation Centre 
Organisation Imperial College London
Country United Kingdom 
Sector Academic/University 
PI Contribution 1) Sectorial workshops with NBIC Company / Academic Community. This gives the opportunity to form links and possible POC projects to be identified. 2) Holding Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. This gives the opportunity to influence NBIC strategy and funding calls. 3) Identifying NBIC fully or part funded Strategic Research (Defined by Sectorial Workshops and approved by Boards). 4) Providing access to POC calls aligned with NBIC Strategy along with Industrial Partner via Sector or Theme. Competitive transparent Open Process Univ signed up to NBIC with companies willing to sign Project Agreement. Selected using range of criteria within a template case. Support in POC submissions and advice from NBIC engagement officers 5) Facilitating collaborative Access to facilities (eg Diamond / Hartree) (Note Science and Industrial Advisory Boards will have regular review and input into these processes and content of themes etc)
Collaborator Contribution 1) Participation in Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. 2) Application to the NBIC Proof of Concept funding for translational research projects 3) Application to studentships and placements with industrial partners
Impact NBIC has awarded 65 Proof of Concept projects, these projects are with the academic partners lists and industry collaborators. We have held three workshop (Biofilm detection, engineering and management), also see publications. The National Biofilms innovation centre is working across disciplines, including: engineering, chemistry, surface and interface science, functional materials, soft matter and non-equilibrium physics, environmental sciences, microbiology, 'omics, bioinformatics, computation and advanced techniques.
Start Year 2018
 
Description National Biofilm Innovation Centre 
Organisation James Hutton Institute
Country United Kingdom 
Sector Charity/Non Profit 
PI Contribution 1) Sectorial workshops with NBIC Company / Academic Community. This gives the opportunity to form links and possible POC projects to be identified. 2) Holding Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. This gives the opportunity to influence NBIC strategy and funding calls. 3) Identifying NBIC fully or part funded Strategic Research (Defined by Sectorial Workshops and approved by Boards). 4) Providing access to POC calls aligned with NBIC Strategy along with Industrial Partner via Sector or Theme. Competitive transparent Open Process Univ signed up to NBIC with companies willing to sign Project Agreement. Selected using range of criteria within a template case. Support in POC submissions and advice from NBIC engagement officers 5) Facilitating collaborative Access to facilities (eg Diamond / Hartree) (Note Science and Industrial Advisory Boards will have regular review and input into these processes and content of themes etc)
Collaborator Contribution 1) Participation in Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. 2) Application to the NBIC Proof of Concept funding for translational research projects 3) Application to studentships and placements with industrial partners
Impact NBIC has awarded 65 Proof of Concept projects, these projects are with the academic partners lists and industry collaborators. We have held three workshop (Biofilm detection, engineering and management), also see publications. The National Biofilms innovation centre is working across disciplines, including: engineering, chemistry, surface and interface science, functional materials, soft matter and non-equilibrium physics, environmental sciences, microbiology, 'omics, bioinformatics, computation and advanced techniques.
Start Year 2018
 
Description National Biofilm Innovation Centre 
Organisation Keele University
Country United Kingdom 
Sector Academic/University 
PI Contribution 1) Sectorial workshops with NBIC Company / Academic Community. This gives the opportunity to form links and possible POC projects to be identified. 2) Holding Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. This gives the opportunity to influence NBIC strategy and funding calls. 3) Identifying NBIC fully or part funded Strategic Research (Defined by Sectorial Workshops and approved by Boards). 4) Providing access to POC calls aligned with NBIC Strategy along with Industrial Partner via Sector or Theme. Competitive transparent Open Process Univ signed up to NBIC with companies willing to sign Project Agreement. Selected using range of criteria within a template case. Support in POC submissions and advice from NBIC engagement officers 5) Facilitating collaborative Access to facilities (eg Diamond / Hartree) (Note Science and Industrial Advisory Boards will have regular review and input into these processes and content of themes etc)
Collaborator Contribution 1) Participation in Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. 2) Application to the NBIC Proof of Concept funding for translational research projects 3) Application to studentships and placements with industrial partners
Impact NBIC has awarded 65 Proof of Concept projects, these projects are with the academic partners lists and industry collaborators. We have held three workshop (Biofilm detection, engineering and management), also see publications. The National Biofilms innovation centre is working across disciplines, including: engineering, chemistry, surface and interface science, functional materials, soft matter and non-equilibrium physics, environmental sciences, microbiology, 'omics, bioinformatics, computation and advanced techniques.
Start Year 2018
 
Description National Biofilm Innovation Centre 
Organisation King's College London
Country United Kingdom 
Sector Academic/University 
PI Contribution 1) Sectorial workshops with NBIC Company / Academic Community. This gives the opportunity to form links and possible POC projects to be identified. 2) Holding Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. This gives the opportunity to influence NBIC strategy and funding calls. 3) Identifying NBIC fully or part funded Strategic Research (Defined by Sectorial Workshops and approved by Boards). 4) Providing access to POC calls aligned with NBIC Strategy along with Industrial Partner via Sector or Theme. Competitive transparent Open Process Univ signed up to NBIC with companies willing to sign Project Agreement. Selected using range of criteria within a template case. Support in POC submissions and advice from NBIC engagement officers 5) Facilitating collaborative Access to facilities (eg Diamond / Hartree) (Note Science and Industrial Advisory Boards will have regular review and input into these processes and content of themes etc)
Collaborator Contribution 1) Participation in Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. 2) Application to the NBIC Proof of Concept funding for translational research projects 3) Application to studentships and placements with industrial partners
Impact NBIC has awarded 65 Proof of Concept projects, these projects are with the academic partners lists and industry collaborators. We have held three workshop (Biofilm detection, engineering and management), also see publications. The National Biofilms innovation centre is working across disciplines, including: engineering, chemistry, surface and interface science, functional materials, soft matter and non-equilibrium physics, environmental sciences, microbiology, 'omics, bioinformatics, computation and advanced techniques.
Start Year 2018
 
Description National Biofilm Innovation Centre 
Organisation Lancaster University
Country United Kingdom 
Sector Academic/University 
PI Contribution 1) Sectorial workshops with NBIC Company / Academic Community. This gives the opportunity to form links and possible POC projects to be identified. 2) Holding Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. This gives the opportunity to influence NBIC strategy and funding calls. 3) Identifying NBIC fully or part funded Strategic Research (Defined by Sectorial Workshops and approved by Boards). 4) Providing access to POC calls aligned with NBIC Strategy along with Industrial Partner via Sector or Theme. Competitive transparent Open Process Univ signed up to NBIC with companies willing to sign Project Agreement. Selected using range of criteria within a template case. Support in POC submissions and advice from NBIC engagement officers 5) Facilitating collaborative Access to facilities (eg Diamond / Hartree) (Note Science and Industrial Advisory Boards will have regular review and input into these processes and content of themes etc)
Collaborator Contribution 1) Participation in Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. 2) Application to the NBIC Proof of Concept funding for translational research projects 3) Application to studentships and placements with industrial partners
Impact NBIC has awarded 65 Proof of Concept projects, these projects are with the academic partners lists and industry collaborators. We have held three workshop (Biofilm detection, engineering and management), also see publications. The National Biofilms innovation centre is working across disciplines, including: engineering, chemistry, surface and interface science, functional materials, soft matter and non-equilibrium physics, environmental sciences, microbiology, 'omics, bioinformatics, computation and advanced techniques.
Start Year 2018
 
Description National Biofilm Innovation Centre 
Organisation Liverpool John Moores University
Country United Kingdom 
Sector Academic/University 
PI Contribution 1) Sectorial workshops with NBIC Company / Academic Community. This gives the opportunity to form links and possible POC projects to be identified. 2) Holding Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. This gives the opportunity to influence NBIC strategy and funding calls. 3) Identifying NBIC fully or part funded Strategic Research (Defined by Sectorial Workshops and approved by Boards). 4) Providing access to POC calls aligned with NBIC Strategy along with Industrial Partner via Sector or Theme. Competitive transparent Open Process Univ signed up to NBIC with companies willing to sign Project Agreement. Selected using range of criteria within a template case. Support in POC submissions and advice from NBIC engagement officers 5) Facilitating collaborative Access to facilities (eg Diamond / Hartree) (Note Science and Industrial Advisory Boards will have regular review and input into these processes and content of themes etc)
Collaborator Contribution 1) Participation in Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. 2) Application to the NBIC Proof of Concept funding for translational research projects 3) Application to studentships and placements with industrial partners
Impact NBIC has awarded 65 Proof of Concept projects, these projects are with the academic partners lists and industry collaborators. We have held three workshop (Biofilm detection, engineering and management), also see publications. The National Biofilms innovation centre is working across disciplines, including: engineering, chemistry, surface and interface science, functional materials, soft matter and non-equilibrium physics, environmental sciences, microbiology, 'omics, bioinformatics, computation and advanced techniques.
Start Year 2018
 
Description National Biofilm Innovation Centre 
Organisation Liverpool School of Tropical Medicine
Country United Kingdom 
Sector Academic/University 
PI Contribution 1) Sectorial workshops with NBIC Company / Academic Community. This gives the opportunity to form links and possible POC projects to be identified. 2) Holding Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. This gives the opportunity to influence NBIC strategy and funding calls. 3) Identifying NBIC fully or part funded Strategic Research (Defined by Sectorial Workshops and approved by Boards). 4) Providing access to POC calls aligned with NBIC Strategy along with Industrial Partner via Sector or Theme. Competitive transparent Open Process Univ signed up to NBIC with companies willing to sign Project Agreement. Selected using range of criteria within a template case. Support in POC submissions and advice from NBIC engagement officers 5) Facilitating collaborative Access to facilities (eg Diamond / Hartree) (Note Science and Industrial Advisory Boards will have regular review and input into these processes and content of themes etc)
Collaborator Contribution 1) Participation in Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. 2) Application to the NBIC Proof of Concept funding for translational research projects 3) Application to studentships and placements with industrial partners
Impact NBIC has awarded 65 Proof of Concept projects, these projects are with the academic partners lists and industry collaborators. We have held three workshop (Biofilm detection, engineering and management), also see publications. The National Biofilms innovation centre is working across disciplines, including: engineering, chemistry, surface and interface science, functional materials, soft matter and non-equilibrium physics, environmental sciences, microbiology, 'omics, bioinformatics, computation and advanced techniques.
Start Year 2018
 
Description National Biofilm Innovation Centre 
Organisation Loughborough University
Country United Kingdom 
Sector Academic/University 
PI Contribution 1) Sectorial workshops with NBIC Company / Academic Community. This gives the opportunity to form links and possible POC projects to be identified. 2) Holding Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. This gives the opportunity to influence NBIC strategy and funding calls. 3) Identifying NBIC fully or part funded Strategic Research (Defined by Sectorial Workshops and approved by Boards). 4) Providing access to POC calls aligned with NBIC Strategy along with Industrial Partner via Sector or Theme. Competitive transparent Open Process Univ signed up to NBIC with companies willing to sign Project Agreement. Selected using range of criteria within a template case. Support in POC submissions and advice from NBIC engagement officers 5) Facilitating collaborative Access to facilities (eg Diamond / Hartree) (Note Science and Industrial Advisory Boards will have regular review and input into these processes and content of themes etc)
Collaborator Contribution 1) Participation in Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. 2) Application to the NBIC Proof of Concept funding for translational research projects 3) Application to studentships and placements with industrial partners
Impact NBIC has awarded 65 Proof of Concept projects, these projects are with the academic partners lists and industry collaborators. We have held three workshop (Biofilm detection, engineering and management), also see publications. The National Biofilms innovation centre is working across disciplines, including: engineering, chemistry, surface and interface science, functional materials, soft matter and non-equilibrium physics, environmental sciences, microbiology, 'omics, bioinformatics, computation and advanced techniques.
Start Year 2018
 
Description National Biofilm Innovation Centre 
Organisation Manchester Metropolitan University
Country United Kingdom 
Sector Academic/University 
PI Contribution 1) Sectorial workshops with NBIC Company / Academic Community. This gives the opportunity to form links and possible POC projects to be identified. 2) Holding Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. This gives the opportunity to influence NBIC strategy and funding calls. 3) Identifying NBIC fully or part funded Strategic Research (Defined by Sectorial Workshops and approved by Boards). 4) Providing access to POC calls aligned with NBIC Strategy along with Industrial Partner via Sector or Theme. Competitive transparent Open Process Univ signed up to NBIC with companies willing to sign Project Agreement. Selected using range of criteria within a template case. Support in POC submissions and advice from NBIC engagement officers 5) Facilitating collaborative Access to facilities (eg Diamond / Hartree) (Note Science and Industrial Advisory Boards will have regular review and input into these processes and content of themes etc)
Collaborator Contribution 1) Participation in Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. 2) Application to the NBIC Proof of Concept funding for translational research projects 3) Application to studentships and placements with industrial partners
Impact NBIC has awarded 65 Proof of Concept projects, these projects are with the academic partners lists and industry collaborators. We have held three workshop (Biofilm detection, engineering and management), also see publications. The National Biofilms innovation centre is working across disciplines, including: engineering, chemistry, surface and interface science, functional materials, soft matter and non-equilibrium physics, environmental sciences, microbiology, 'omics, bioinformatics, computation and advanced techniques.
Start Year 2018
 
Description National Biofilm Innovation Centre 
Organisation Newcastle University
Country United Kingdom 
Sector Academic/University 
PI Contribution 1) Sectorial workshops with NBIC Company / Academic Community. This gives the opportunity to form links and possible POC projects to be identified. 2) Holding Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. This gives the opportunity to influence NBIC strategy and funding calls. 3) Identifying NBIC fully or part funded Strategic Research (Defined by Sectorial Workshops and approved by Boards). 4) Providing access to POC calls aligned with NBIC Strategy along with Industrial Partner via Sector or Theme. Competitive transparent Open Process Univ signed up to NBIC with companies willing to sign Project Agreement. Selected using range of criteria within a template case. Support in POC submissions and advice from NBIC engagement officers 5) Facilitating collaborative Access to facilities (eg Diamond / Hartree) (Note Science and Industrial Advisory Boards will have regular review and input into these processes and content of themes etc)
Collaborator Contribution 1) Participation in Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. 2) Application to the NBIC Proof of Concept funding for translational research projects 3) Application to studentships and placements with industrial partners
Impact NBIC has awarded 65 Proof of Concept projects, these projects are with the academic partners lists and industry collaborators. We have held three workshop (Biofilm detection, engineering and management), also see publications. The National Biofilms innovation centre is working across disciplines, including: engineering, chemistry, surface and interface science, functional materials, soft matter and non-equilibrium physics, environmental sciences, microbiology, 'omics, bioinformatics, computation and advanced techniques.
Start Year 2018
 
Description National Biofilm Innovation Centre 
Organisation Nottingham Trent University
Country United Kingdom 
Sector Academic/University 
PI Contribution 1) Sectorial workshops with NBIC Company / Academic Community. This gives the opportunity to form links and possible POC projects to be identified. 2) Holding Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. This gives the opportunity to influence NBIC strategy and funding calls. 3) Identifying NBIC fully or part funded Strategic Research (Defined by Sectorial Workshops and approved by Boards). 4) Providing access to POC calls aligned with NBIC Strategy along with Industrial Partner via Sector or Theme. Competitive transparent Open Process Univ signed up to NBIC with companies willing to sign Project Agreement. Selected using range of criteria within a template case. Support in POC submissions and advice from NBIC engagement officers 5) Facilitating collaborative Access to facilities (eg Diamond / Hartree) (Note Science and Industrial Advisory Boards will have regular review and input into these processes and content of themes etc)
Collaborator Contribution 1) Participation in Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. 2) Application to the NBIC Proof of Concept funding for translational research projects 3) Application to studentships and placements with industrial partners
Impact NBIC has awarded 65 Proof of Concept projects, these projects are with the academic partners lists and industry collaborators. We have held three workshop (Biofilm detection, engineering and management), also see publications. The National Biofilms innovation centre is working across disciplines, including: engineering, chemistry, surface and interface science, functional materials, soft matter and non-equilibrium physics, environmental sciences, microbiology, 'omics, bioinformatics, computation and advanced techniques.
Start Year 2018
 
Description National Biofilm Innovation Centre 
Organisation Plymouth Marine Laboratory
Country United Kingdom 
Sector Academic/University 
PI Contribution 1) Sectorial workshops with NBIC Company / Academic Community. This gives the opportunity to form links and possible POC projects to be identified. 2) Holding Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. This gives the opportunity to influence NBIC strategy and funding calls. 3) Identifying NBIC fully or part funded Strategic Research (Defined by Sectorial Workshops and approved by Boards). 4) Providing access to POC calls aligned with NBIC Strategy along with Industrial Partner via Sector or Theme. Competitive transparent Open Process Univ signed up to NBIC with companies willing to sign Project Agreement. Selected using range of criteria within a template case. Support in POC submissions and advice from NBIC engagement officers 5) Facilitating collaborative Access to facilities (eg Diamond / Hartree) (Note Science and Industrial Advisory Boards will have regular review and input into these processes and content of themes etc)
Collaborator Contribution 1) Participation in Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. 2) Application to the NBIC Proof of Concept funding for translational research projects 3) Application to studentships and placements with industrial partners
Impact NBIC has awarded 65 Proof of Concept projects, these projects are with the academic partners lists and industry collaborators. We have held three workshop (Biofilm detection, engineering and management), also see publications. The National Biofilms innovation centre is working across disciplines, including: engineering, chemistry, surface and interface science, functional materials, soft matter and non-equilibrium physics, environmental sciences, microbiology, 'omics, bioinformatics, computation and advanced techniques.
Start Year 2018
 
Description National Biofilm Innovation Centre 
Organisation Quadram Institute Bioscience
Country United Kingdom 
Sector Academic/University 
PI Contribution 1) Sectorial workshops with NBIC Company / Academic Community. This gives the opportunity to form links and possible POC projects to be identified. 2) Holding Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. This gives the opportunity to influence NBIC strategy and funding calls. 3) Identifying NBIC fully or part funded Strategic Research (Defined by Sectorial Workshops and approved by Boards). 4) Providing access to POC calls aligned with NBIC Strategy along with Industrial Partner via Sector or Theme. Competitive transparent Open Process Univ signed up to NBIC with companies willing to sign Project Agreement. Selected using range of criteria within a template case. Support in POC submissions and advice from NBIC engagement officers 5) Facilitating collaborative Access to facilities (eg Diamond / Hartree) (Note Science and Industrial Advisory Boards will have regular review and input into these processes and content of themes etc)
Collaborator Contribution 1) Participation in Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. 2) Application to the NBIC Proof of Concept funding for translational research projects 3) Application to studentships and placements with industrial partners
Impact NBIC has awarded 65 Proof of Concept projects, these projects are with the academic partners lists and industry collaborators. We have held three workshop (Biofilm detection, engineering and management), also see publications. The National Biofilms innovation centre is working across disciplines, including: engineering, chemistry, surface and interface science, functional materials, soft matter and non-equilibrium physics, environmental sciences, microbiology, 'omics, bioinformatics, computation and advanced techniques.
Start Year 2018
 
Description National Biofilm Innovation Centre 
Organisation Queen Mary University of London
Country United Kingdom 
Sector Academic/University 
PI Contribution 1) Sectorial workshops with NBIC Company / Academic Community. This gives the opportunity to form links and possible POC projects to be identified. 2) Holding Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. This gives the opportunity to influence NBIC strategy and funding calls. 3) Identifying NBIC fully or part funded Strategic Research (Defined by Sectorial Workshops and approved by Boards). 4) Providing access to POC calls aligned with NBIC Strategy along with Industrial Partner via Sector or Theme. Competitive transparent Open Process Univ signed up to NBIC with companies willing to sign Project Agreement. Selected using range of criteria within a template case. Support in POC submissions and advice from NBIC engagement officers 5) Facilitating collaborative Access to facilities (eg Diamond / Hartree) (Note Science and Industrial Advisory Boards will have regular review and input into these processes and content of themes etc)
Collaborator Contribution 1) Participation in Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. 2) Application to the NBIC Proof of Concept funding for translational research projects 3) Application to studentships and placements with industrial partners
Impact NBIC has awarded 65 Proof of Concept projects, these projects are with the academic partners lists and industry collaborators. We have held three workshop (Biofilm detection, engineering and management), also see publications. The National Biofilms innovation centre is working across disciplines, including: engineering, chemistry, surface and interface science, functional materials, soft matter and non-equilibrium physics, environmental sciences, microbiology, 'omics, bioinformatics, computation and advanced techniques.
Start Year 2018
 
Description National Biofilm Innovation Centre 
Organisation Sheffield Hallam University
Country United Kingdom 
Sector Academic/University 
PI Contribution 1) Sectorial workshops with NBIC Company / Academic Community. This gives the opportunity to form links and possible POC projects to be identified. 2) Holding Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. This gives the opportunity to influence NBIC strategy and funding calls. 3) Identifying NBIC fully or part funded Strategic Research (Defined by Sectorial Workshops and approved by Boards). 4) Providing access to POC calls aligned with NBIC Strategy along with Industrial Partner via Sector or Theme. Competitive transparent Open Process Univ signed up to NBIC with companies willing to sign Project Agreement. Selected using range of criteria within a template case. Support in POC submissions and advice from NBIC engagement officers 5) Facilitating collaborative Access to facilities (eg Diamond / Hartree) (Note Science and Industrial Advisory Boards will have regular review and input into these processes and content of themes etc)
Collaborator Contribution 1) Participation in Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. 2) Application to the NBIC Proof of Concept funding for translational research projects 3) Application to studentships and placements with industrial partners
Impact NBIC has awarded 65 Proof of Concept projects, these projects are with the academic partners lists and industry collaborators. We have held three workshop (Biofilm detection, engineering and management), also see publications. The National Biofilms innovation centre is working across disciplines, including: engineering, chemistry, surface and interface science, functional materials, soft matter and non-equilibrium physics, environmental sciences, microbiology, 'omics, bioinformatics, computation and advanced techniques.
Start Year 2018
 
Description National Biofilm Innovation Centre 
Organisation Swansea University
Country United Kingdom 
Sector Academic/University 
PI Contribution 1) Sectorial workshops with NBIC Company / Academic Community. This gives the opportunity to form links and possible POC projects to be identified. 2) Holding Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. This gives the opportunity to influence NBIC strategy and funding calls. 3) Identifying NBIC fully or part funded Strategic Research (Defined by Sectorial Workshops and approved by Boards). 4) Providing access to POC calls aligned with NBIC Strategy along with Industrial Partner via Sector or Theme. Competitive transparent Open Process Univ signed up to NBIC with companies willing to sign Project Agreement. Selected using range of criteria within a template case. Support in POC submissions and advice from NBIC engagement officers 5) Facilitating collaborative Access to facilities (eg Diamond / Hartree) (Note Science and Industrial Advisory Boards will have regular review and input into these processes and content of themes etc)
Collaborator Contribution 1) Participation in Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. 2) Application to the NBIC Proof of Concept funding for translational research projects 3) Application to studentships and placements with industrial partners
Impact NBIC has awarded 65 Proof of Concept projects, these projects are with the academic partners lists and industry collaborators. We have held three workshop (Biofilm detection, engineering and management), also see publications. The National Biofilms innovation centre is working across disciplines, including: engineering, chemistry, surface and interface science, functional materials, soft matter and non-equilibrium physics, environmental sciences, microbiology, 'omics, bioinformatics, computation and advanced techniques.
Start Year 2018
 
Description National Biofilm Innovation Centre 
Organisation Teesside University
Country United Kingdom 
Sector Academic/University 
PI Contribution 1) Sectorial workshops with NBIC Company / Academic Community. This gives the opportunity to form links and possible POC projects to be identified. 2) Holding Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. This gives the opportunity to influence NBIC strategy and funding calls. 3) Identifying NBIC fully or part funded Strategic Research (Defined by Sectorial Workshops and approved by Boards). 4) Providing access to POC calls aligned with NBIC Strategy along with Industrial Partner via Sector or Theme. Competitive transparent Open Process Univ signed up to NBIC with companies willing to sign Project Agreement. Selected using range of criteria within a template case. Support in POC submissions and advice from NBIC engagement officers 5) Facilitating collaborative Access to facilities (eg Diamond / Hartree) (Note Science and Industrial Advisory Boards will have regular review and input into these processes and content of themes etc)
Collaborator Contribution 1) Participation in Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. 2) Application to the NBIC Proof of Concept funding for translational research projects 3) Application to studentships and placements with industrial partners
Impact NBIC has awarded 65 Proof of Concept projects, these projects are with the academic partners lists and industry collaborators. We have held three workshop (Biofilm detection, engineering and management), also see publications. The National Biofilms innovation centre is working across disciplines, including: engineering, chemistry, surface and interface science, functional materials, soft matter and non-equilibrium physics, environmental sciences, microbiology, 'omics, bioinformatics, computation and advanced techniques.
Start Year 2018
 
Description National Biofilm Innovation Centre 
Organisation University of Aberdeen
Country United Kingdom 
Sector Academic/University 
PI Contribution 1) Sectorial workshops with NBIC Company / Academic Community. This gives the opportunity to form links and possible POC projects to be identified. 2) Holding Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. This gives the opportunity to influence NBIC strategy and funding calls. 3) Identifying NBIC fully or part funded Strategic Research (Defined by Sectorial Workshops and approved by Boards). 4) Providing access to POC calls aligned with NBIC Strategy along with Industrial Partner via Sector or Theme. Competitive transparent Open Process Univ signed up to NBIC with companies willing to sign Project Agreement. Selected using range of criteria within a template case. Support in POC submissions and advice from NBIC engagement officers 5) Facilitating collaborative Access to facilities (eg Diamond / Hartree) (Note Science and Industrial Advisory Boards will have regular review and input into these processes and content of themes etc)
Collaborator Contribution 1) Participation in Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. 2) Application to the NBIC Proof of Concept funding for translational research projects 3) Application to studentships and placements with industrial partners
Impact NBIC has awarded 65 Proof of Concept projects, these projects are with the academic partners lists and industry collaborators. We have held three workshop (Biofilm detection, engineering and management), also see publications. The National Biofilms innovation centre is working across disciplines, including: engineering, chemistry, surface and interface science, functional materials, soft matter and non-equilibrium physics, environmental sciences, microbiology, 'omics, bioinformatics, computation and advanced techniques.
Start Year 2018
 
Description National Biofilm Innovation Centre 
Organisation University of Bath
Country United Kingdom 
Sector Academic/University 
PI Contribution 1) Sectorial workshops with NBIC Company / Academic Community. This gives the opportunity to form links and possible POC projects to be identified. 2) Holding Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. This gives the opportunity to influence NBIC strategy and funding calls. 3) Identifying NBIC fully or part funded Strategic Research (Defined by Sectorial Workshops and approved by Boards). 4) Providing access to POC calls aligned with NBIC Strategy along with Industrial Partner via Sector or Theme. Competitive transparent Open Process Univ signed up to NBIC with companies willing to sign Project Agreement. Selected using range of criteria within a template case. Support in POC submissions and advice from NBIC engagement officers 5) Facilitating collaborative Access to facilities (eg Diamond / Hartree) (Note Science and Industrial Advisory Boards will have regular review and input into these processes and content of themes etc)
Collaborator Contribution 1) Participation in Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. 2) Application to the NBIC Proof of Concept funding for translational research projects 3) Application to studentships and placements with industrial partners
Impact NBIC has awarded 65 Proof of Concept projects, these projects are with the academic partners lists and industry collaborators. We have held three workshop (Biofilm detection, engineering and management), also see publications. The National Biofilms innovation centre is working across disciplines, including: engineering, chemistry, surface and interface science, functional materials, soft matter and non-equilibrium physics, environmental sciences, microbiology, 'omics, bioinformatics, computation and advanced techniques.
Start Year 2018
 
Description National Biofilm Innovation Centre 
Organisation University of Birmingham
Country United Kingdom 
Sector Academic/University 
PI Contribution 1) Sectorial workshops with NBIC Company / Academic Community. This gives the opportunity to form links and possible POC projects to be identified. 2) Holding Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. This gives the opportunity to influence NBIC strategy and funding calls. 3) Identifying NBIC fully or part funded Strategic Research (Defined by Sectorial Workshops and approved by Boards). 4) Providing access to POC calls aligned with NBIC Strategy along with Industrial Partner via Sector or Theme. Competitive transparent Open Process Univ signed up to NBIC with companies willing to sign Project Agreement. Selected using range of criteria within a template case. Support in POC submissions and advice from NBIC engagement officers 5) Facilitating collaborative Access to facilities (eg Diamond / Hartree) (Note Science and Industrial Advisory Boards will have regular review and input into these processes and content of themes etc)
Collaborator Contribution 1) Participation in Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. 2) Application to the NBIC Proof of Concept funding for translational research projects 3) Application to studentships and placements with industrial partners
Impact NBIC has awarded 65 Proof of Concept projects, these projects are with the academic partners lists and industry collaborators. We have held three workshop (Biofilm detection, engineering and management), also see publications. The National Biofilms innovation centre is working across disciplines, including: engineering, chemistry, surface and interface science, functional materials, soft matter and non-equilibrium physics, environmental sciences, microbiology, 'omics, bioinformatics, computation and advanced techniques.
Start Year 2018
 
Description National Biofilm Innovation Centre 
Organisation University of Bradford
Country United Kingdom 
Sector Academic/University 
PI Contribution 1) Sectorial workshops with NBIC Company / Academic Community. This gives the opportunity to form links and possible POC projects to be identified. 2) Holding Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. This gives the opportunity to influence NBIC strategy and funding calls. 3) Identifying NBIC fully or part funded Strategic Research (Defined by Sectorial Workshops and approved by Boards). 4) Providing access to POC calls aligned with NBIC Strategy along with Industrial Partner via Sector or Theme. Competitive transparent Open Process Univ signed up to NBIC with companies willing to sign Project Agreement. Selected using range of criteria within a template case. Support in POC submissions and advice from NBIC engagement officers 5) Facilitating collaborative Access to facilities (eg Diamond / Hartree) (Note Science and Industrial Advisory Boards will have regular review and input into these processes and content of themes etc)
Collaborator Contribution 1) Participation in Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. 2) Application to the NBIC Proof of Concept funding for translational research projects 3) Application to studentships and placements with industrial partners
Impact NBIC has awarded 65 Proof of Concept projects, these projects are with the academic partners lists and industry collaborators. We have held three workshop (Biofilm detection, engineering and management), also see publications. The National Biofilms innovation centre is working across disciplines, including: engineering, chemistry, surface and interface science, functional materials, soft matter and non-equilibrium physics, environmental sciences, microbiology, 'omics, bioinformatics, computation and advanced techniques.
Start Year 2018
 
Description National Biofilm Innovation Centre 
Organisation University of Bristol
Country United Kingdom 
Sector Academic/University 
PI Contribution 1) Sectorial workshops with NBIC Company / Academic Community. This gives the opportunity to form links and possible POC projects to be identified. 2) Holding Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. This gives the opportunity to influence NBIC strategy and funding calls. 3) Identifying NBIC fully or part funded Strategic Research (Defined by Sectorial Workshops and approved by Boards). 4) Providing access to POC calls aligned with NBIC Strategy along with Industrial Partner via Sector or Theme. Competitive transparent Open Process Univ signed up to NBIC with companies willing to sign Project Agreement. Selected using range of criteria within a template case. Support in POC submissions and advice from NBIC engagement officers 5) Facilitating collaborative Access to facilities (eg Diamond / Hartree) (Note Science and Industrial Advisory Boards will have regular review and input into these processes and content of themes etc)
Collaborator Contribution 1) Participation in Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. 2) Application to the NBIC Proof of Concept funding for translational research projects 3) Application to studentships and placements with industrial partners
Impact NBIC has awarded 65 Proof of Concept projects, these projects are with the academic partners lists and industry collaborators. We have held three workshop (Biofilm detection, engineering and management), also see publications. The National Biofilms innovation centre is working across disciplines, including: engineering, chemistry, surface and interface science, functional materials, soft matter and non-equilibrium physics, environmental sciences, microbiology, 'omics, bioinformatics, computation and advanced techniques.
Start Year 2018
 
Description National Biofilm Innovation Centre 
Organisation University of Cambridge
Country United Kingdom 
Sector Academic/University 
PI Contribution 1) Sectorial workshops with NBIC Company / Academic Community. This gives the opportunity to form links and possible POC projects to be identified. 2) Holding Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. This gives the opportunity to influence NBIC strategy and funding calls. 3) Identifying NBIC fully or part funded Strategic Research (Defined by Sectorial Workshops and approved by Boards). 4) Providing access to POC calls aligned with NBIC Strategy along with Industrial Partner via Sector or Theme. Competitive transparent Open Process Univ signed up to NBIC with companies willing to sign Project Agreement. Selected using range of criteria within a template case. Support in POC submissions and advice from NBIC engagement officers 5) Facilitating collaborative Access to facilities (eg Diamond / Hartree) (Note Science and Industrial Advisory Boards will have regular review and input into these processes and content of themes etc)
Collaborator Contribution 1) Participation in Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. 2) Application to the NBIC Proof of Concept funding for translational research projects 3) Application to studentships and placements with industrial partners
Impact NBIC has awarded 65 Proof of Concept projects, these projects are with the academic partners lists and industry collaborators. We have held three workshop (Biofilm detection, engineering and management), also see publications. The National Biofilms innovation centre is working across disciplines, including: engineering, chemistry, surface and interface science, functional materials, soft matter and non-equilibrium physics, environmental sciences, microbiology, 'omics, bioinformatics, computation and advanced techniques.
Start Year 2018
 
Description National Biofilm Innovation Centre 
Organisation University of Dundee
Country United Kingdom 
Sector Academic/University 
PI Contribution 1) Sectorial workshops with NBIC Company / Academic Community. This gives the opportunity to form links and possible POC projects to be identified. 2) Holding Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. This gives the opportunity to influence NBIC strategy and funding calls. 3) Identifying NBIC fully or part funded Strategic Research (Defined by Sectorial Workshops and approved by Boards). 4) Providing access to POC calls aligned with NBIC Strategy along with Industrial Partner via Sector or Theme. Competitive transparent Open Process Univ signed up to NBIC with companies willing to sign Project Agreement. Selected using range of criteria within a template case. Support in POC submissions and advice from NBIC engagement officers 5) Facilitating collaborative Access to facilities (eg Diamond / Hartree) (Note Science and Industrial Advisory Boards will have regular review and input into these processes and content of themes etc)
Collaborator Contribution 1) Participation in Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. 2) Application to the NBIC Proof of Concept funding for translational research projects 3) Application to studentships and placements with industrial partners
Impact NBIC has awarded 65 Proof of Concept projects, these projects are with the academic partners lists and industry collaborators. We have held three workshop (Biofilm detection, engineering and management), also see publications. The National Biofilms innovation centre is working across disciplines, including: engineering, chemistry, surface and interface science, functional materials, soft matter and non-equilibrium physics, environmental sciences, microbiology, 'omics, bioinformatics, computation and advanced techniques.
Start Year 2018
 
Description National Biofilm Innovation Centre 
Organisation University of East Anglia
Country United Kingdom 
Sector Academic/University 
PI Contribution 1) Sectorial workshops with NBIC Company / Academic Community. This gives the opportunity to form links and possible POC projects to be identified. 2) Holding Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. This gives the opportunity to influence NBIC strategy and funding calls. 3) Identifying NBIC fully or part funded Strategic Research (Defined by Sectorial Workshops and approved by Boards). 4) Providing access to POC calls aligned with NBIC Strategy along with Industrial Partner via Sector or Theme. Competitive transparent Open Process Univ signed up to NBIC with companies willing to sign Project Agreement. Selected using range of criteria within a template case. Support in POC submissions and advice from NBIC engagement officers 5) Facilitating collaborative Access to facilities (eg Diamond / Hartree) (Note Science and Industrial Advisory Boards will have regular review and input into these processes and content of themes etc)
Collaborator Contribution 1) Participation in Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. 2) Application to the NBIC Proof of Concept funding for translational research projects 3) Application to studentships and placements with industrial partners
Impact NBIC has awarded 65 Proof of Concept projects, these projects are with the academic partners lists and industry collaborators. We have held three workshop (Biofilm detection, engineering and management), also see publications. The National Biofilms innovation centre is working across disciplines, including: engineering, chemistry, surface and interface science, functional materials, soft matter and non-equilibrium physics, environmental sciences, microbiology, 'omics, bioinformatics, computation and advanced techniques.
Start Year 2018
 
Description National Biofilm Innovation Centre 
Organisation University of Edinburgh
Country United Kingdom 
Sector Academic/University 
PI Contribution 1) Sectorial workshops with NBIC Company / Academic Community. This gives the opportunity to form links and possible POC projects to be identified. 2) Holding Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. This gives the opportunity to influence NBIC strategy and funding calls. 3) Identifying NBIC fully or part funded Strategic Research (Defined by Sectorial Workshops and approved by Boards). 4) Providing access to POC calls aligned with NBIC Strategy along with Industrial Partner via Sector or Theme. Competitive transparent Open Process Univ signed up to NBIC with companies willing to sign Project Agreement. Selected using range of criteria within a template case. Support in POC submissions and advice from NBIC engagement officers 5) Facilitating collaborative Access to facilities (eg Diamond / Hartree) (Note Science and Industrial Advisory Boards will have regular review and input into these processes and content of themes etc)
Collaborator Contribution 1) Participation in Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. 2) Application to the NBIC Proof of Concept funding for translational research projects 3) Application to studentships and placements with industrial partners
Impact NBIC has awarded 65 Proof of Concept projects, these projects are with the academic partners lists and industry collaborators. We have held three workshop (Biofilm detection, engineering and management), also see publications. The National Biofilms innovation centre is working across disciplines, including: engineering, chemistry, surface and interface science, functional materials, soft matter and non-equilibrium physics, environmental sciences, microbiology, 'omics, bioinformatics, computation and advanced techniques.
Start Year 2018
 
Description National Biofilm Innovation Centre 
Organisation University of Essex
Country United Kingdom 
Sector Academic/University 
PI Contribution 1) Sectorial workshops with NBIC Company / Academic Community. This gives the opportunity to form links and possible POC projects to be identified. 2) Holding Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. This gives the opportunity to influence NBIC strategy and funding calls. 3) Identifying NBIC fully or part funded Strategic Research (Defined by Sectorial Workshops and approved by Boards). 4) Providing access to POC calls aligned with NBIC Strategy along with Industrial Partner via Sector or Theme. Competitive transparent Open Process Univ signed up to NBIC with companies willing to sign Project Agreement. Selected using range of criteria within a template case. Support in POC submissions and advice from NBIC engagement officers 5) Facilitating collaborative Access to facilities (eg Diamond / Hartree) (Note Science and Industrial Advisory Boards will have regular review and input into these processes and content of themes etc)
Collaborator Contribution 1) Participation in Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. 2) Application to the NBIC Proof of Concept funding for translational research projects 3) Application to studentships and placements with industrial partners
Impact NBIC has awarded 65 Proof of Concept projects, these projects are with the academic partners lists and industry collaborators. We have held three workshop (Biofilm detection, engineering and management), also see publications. The National Biofilms innovation centre is working across disciplines, including: engineering, chemistry, surface and interface science, functional materials, soft matter and non-equilibrium physics, environmental sciences, microbiology, 'omics, bioinformatics, computation and advanced techniques.
Start Year 2018
 
Description National Biofilm Innovation Centre 
Organisation University of Exeter
Country United Kingdom 
Sector Academic/University 
PI Contribution 1) Sectorial workshops with NBIC Company / Academic Community. This gives the opportunity to form links and possible POC projects to be identified. 2) Holding Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. This gives the opportunity to influence NBIC strategy and funding calls. 3) Identifying NBIC fully or part funded Strategic Research (Defined by Sectorial Workshops and approved by Boards). 4) Providing access to POC calls aligned with NBIC Strategy along with Industrial Partner via Sector or Theme. Competitive transparent Open Process Univ signed up to NBIC with companies willing to sign Project Agreement. Selected using range of criteria within a template case. Support in POC submissions and advice from NBIC engagement officers 5) Facilitating collaborative Access to facilities (eg Diamond / Hartree) (Note Science and Industrial Advisory Boards will have regular review and input into these processes and content of themes etc)
Collaborator Contribution 1) Participation in Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. 2) Application to the NBIC Proof of Concept funding for translational research projects 3) Application to studentships and placements with industrial partners
Impact NBIC has awarded 65 Proof of Concept projects, these projects are with the academic partners lists and industry collaborators. We have held three workshop (Biofilm detection, engineering and management), also see publications. The National Biofilms innovation centre is working across disciplines, including: engineering, chemistry, surface and interface science, functional materials, soft matter and non-equilibrium physics, environmental sciences, microbiology, 'omics, bioinformatics, computation and advanced techniques.
Start Year 2018
 
Description National Biofilm Innovation Centre 
Organisation University of Glasgow
Country United Kingdom 
Sector Academic/University 
PI Contribution 1) Sectorial workshops with NBIC Company / Academic Community. This gives the opportunity to form links and possible POC projects to be identified. 2) Holding Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. This gives the opportunity to influence NBIC strategy and funding calls. 3) Identifying NBIC fully or part funded Strategic Research (Defined by Sectorial Workshops and approved by Boards). 4) Providing access to POC calls aligned with NBIC Strategy along with Industrial Partner via Sector or Theme. Competitive transparent Open Process Univ signed up to NBIC with companies willing to sign Project Agreement. Selected using range of criteria within a template case. Support in POC submissions and advice from NBIC engagement officers 5) Facilitating collaborative Access to facilities (eg Diamond / Hartree) (Note Science and Industrial Advisory Boards will have regular review and input into these processes and content of themes etc)
Collaborator Contribution 1) Participation in Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. 2) Application to the NBIC Proof of Concept funding for translational research projects 3) Application to studentships and placements with industrial partners
Impact NBIC has awarded 65 Proof of Concept projects, these projects are with the academic partners lists and industry collaborators. We have held three workshop (Biofilm detection, engineering and management), also see publications. The National Biofilms innovation centre is working across disciplines, including: engineering, chemistry, surface and interface science, functional materials, soft matter and non-equilibrium physics, environmental sciences, microbiology, 'omics, bioinformatics, computation and advanced techniques.
Start Year 2018
 
Description National Biofilm Innovation Centre 
Organisation University of Hertfordshire
Country United Kingdom 
Sector Academic/University 
PI Contribution 1) Sectorial workshops with NBIC Company / Academic Community. This gives the opportunity to form links and possible POC projects to be identified. 2) Holding Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. This gives the opportunity to influence NBIC strategy and funding calls. 3) Identifying NBIC fully or part funded Strategic Research (Defined by Sectorial Workshops and approved by Boards). 4) Providing access to POC calls aligned with NBIC Strategy along with Industrial Partner via Sector or Theme. Competitive transparent Open Process Univ signed up to NBIC with companies willing to sign Project Agreement. Selected using range of criteria within a template case. Support in POC submissions and advice from NBIC engagement officers 5) Facilitating collaborative Access to facilities (eg Diamond / Hartree) (Note Science and Industrial Advisory Boards will have regular review and input into these processes and content of themes etc)
Collaborator Contribution 1) Participation in Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. 2) Application to the NBIC Proof of Concept funding for translational research projects 3) Application to studentships and placements with industrial partners
Impact NBIC has awarded 65 Proof of Concept projects, these projects are with the academic partners lists and industry collaborators. We have held three workshop (Biofilm detection, engineering and management), also see publications. The National Biofilms innovation centre is working across disciplines, including: engineering, chemistry, surface and interface science, functional materials, soft matter and non-equilibrium physics, environmental sciences, microbiology, 'omics, bioinformatics, computation and advanced techniques.
Start Year 2018
 
Description National Biofilm Innovation Centre 
Organisation University of Huddersfield
Country United Kingdom 
Sector Academic/University 
PI Contribution 1) Sectorial workshops with NBIC Company / Academic Community. This gives the opportunity to form links and possible POC projects to be identified. 2) Holding Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. This gives the opportunity to influence NBIC strategy and funding calls. 3) Identifying NBIC fully or part funded Strategic Research (Defined by Sectorial Workshops and approved by Boards). 4) Providing access to POC calls aligned with NBIC Strategy along with Industrial Partner via Sector or Theme. Competitive transparent Open Process Univ signed up to NBIC with companies willing to sign Project Agreement. Selected using range of criteria within a template case. Support in POC submissions and advice from NBIC engagement officers 5) Facilitating collaborative Access to facilities (eg Diamond / Hartree) (Note Science and Industrial Advisory Boards will have regular review and input into these processes and content of themes etc)
Collaborator Contribution 1) Participation in Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. 2) Application to the NBIC Proof of Concept funding for translational research projects 3) Application to studentships and placements with industrial partners
Impact NBIC has awarded 65 Proof of Concept projects, these projects are with the academic partners lists and industry collaborators. We have held three workshop (Biofilm detection, engineering and management), also see publications. The National Biofilms innovation centre is working across disciplines, including: engineering, chemistry, surface and interface science, functional materials, soft matter and non-equilibrium physics, environmental sciences, microbiology, 'omics, bioinformatics, computation and advanced techniques.
Start Year 2018
 
Description National Biofilm Innovation Centre 
Organisation University of Hull
Country United Kingdom 
Sector Academic/University 
PI Contribution 1) Sectorial workshops with NBIC Company / Academic Community. This gives the opportunity to form links and possible POC projects to be identified. 2) Holding Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. This gives the opportunity to influence NBIC strategy and funding calls. 3) Identifying NBIC fully or part funded Strategic Research (Defined by Sectorial Workshops and approved by Boards). 4) Providing access to POC calls aligned with NBIC Strategy along with Industrial Partner via Sector or Theme. Competitive transparent Open Process Univ signed up to NBIC with companies willing to sign Project Agreement. Selected using range of criteria within a template case. Support in POC submissions and advice from NBIC engagement officers 5) Facilitating collaborative Access to facilities (eg Diamond / Hartree) (Note Science and Industrial Advisory Boards will have regular review and input into these processes and content of themes etc)
Collaborator Contribution 1) Participation in Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. 2) Application to the NBIC Proof of Concept funding for translational research projects 3) Application to studentships and placements with industrial partners
Impact NBIC has awarded 65 Proof of Concept projects, these projects are with the academic partners lists and industry collaborators. We have held three workshop (Biofilm detection, engineering and management), also see publications. The National Biofilms innovation centre is working across disciplines, including: engineering, chemistry, surface and interface science, functional materials, soft matter and non-equilibrium physics, environmental sciences, microbiology, 'omics, bioinformatics, computation and advanced techniques.
Start Year 2018
 
Description National Biofilm Innovation Centre 
Organisation University of Kent
Country United Kingdom 
Sector Academic/University 
PI Contribution 1) Sectorial workshops with NBIC Company / Academic Community. This gives the opportunity to form links and possible POC projects to be identified. 2) Holding Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. This gives the opportunity to influence NBIC strategy and funding calls. 3) Identifying NBIC fully or part funded Strategic Research (Defined by Sectorial Workshops and approved by Boards). 4) Providing access to POC calls aligned with NBIC Strategy along with Industrial Partner via Sector or Theme. Competitive transparent Open Process Univ signed up to NBIC with companies willing to sign Project Agreement. Selected using range of criteria within a template case. Support in POC submissions and advice from NBIC engagement officers 5) Facilitating collaborative Access to facilities (eg Diamond / Hartree) (Note Science and Industrial Advisory Boards will have regular review and input into these processes and content of themes etc)
Collaborator Contribution 1) Participation in Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. 2) Application to the NBIC Proof of Concept funding for translational research projects 3) Application to studentships and placements with industrial partners
Impact NBIC has awarded 65 Proof of Concept projects, these projects are with the academic partners lists and industry collaborators. We have held three workshop (Biofilm detection, engineering and management), also see publications. The National Biofilms innovation centre is working across disciplines, including: engineering, chemistry, surface and interface science, functional materials, soft matter and non-equilibrium physics, environmental sciences, microbiology, 'omics, bioinformatics, computation and advanced techniques.
Start Year 2018
 
Description National Biofilm Innovation Centre 
Organisation University of Leeds
Country United Kingdom 
Sector Academic/University 
PI Contribution 1) Sectorial workshops with NBIC Company / Academic Community. This gives the opportunity to form links and possible POC projects to be identified. 2) Holding Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. This gives the opportunity to influence NBIC strategy and funding calls. 3) Identifying NBIC fully or part funded Strategic Research (Defined by Sectorial Workshops and approved by Boards). 4) Providing access to POC calls aligned with NBIC Strategy along with Industrial Partner via Sector or Theme. Competitive transparent Open Process Univ signed up to NBIC with companies willing to sign Project Agreement. Selected using range of criteria within a template case. Support in POC submissions and advice from NBIC engagement officers 5) Facilitating collaborative Access to facilities (eg Diamond / Hartree) (Note Science and Industrial Advisory Boards will have regular review and input into these processes and content of themes etc)
Collaborator Contribution 1) Participation in Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. 2) Application to the NBIC Proof of Concept funding for translational research projects 3) Application to studentships and placements with industrial partners
Impact NBIC has awarded 65 Proof of Concept projects, these projects are with the academic partners lists and industry collaborators. We have held three workshop (Biofilm detection, engineering and management), also see publications. The National Biofilms innovation centre is working across disciplines, including: engineering, chemistry, surface and interface science, functional materials, soft matter and non-equilibrium physics, environmental sciences, microbiology, 'omics, bioinformatics, computation and advanced techniques.
Start Year 2018
 
Description National Biofilm Innovation Centre 
Organisation University of Lincoln
Country United Kingdom 
Sector Academic/University 
PI Contribution 1) Sectorial workshops with NBIC Company / Academic Community. This gives the opportunity to form links and possible POC projects to be identified. 2) Holding Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. This gives the opportunity to influence NBIC strategy and funding calls. 3) Identifying NBIC fully or part funded Strategic Research (Defined by Sectorial Workshops and approved by Boards). 4) Providing access to POC calls aligned with NBIC Strategy along with Industrial Partner via Sector or Theme. Competitive transparent Open Process Univ signed up to NBIC with companies willing to sign Project Agreement. Selected using range of criteria within a template case. Support in POC submissions and advice from NBIC engagement officers 5) Facilitating collaborative Access to facilities (eg Diamond / Hartree) (Note Science and Industrial Advisory Boards will have regular review and input into these processes and content of themes etc)
Collaborator Contribution 1) Participation in Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. 2) Application to the NBIC Proof of Concept funding for translational research projects 3) Application to studentships and placements with industrial partners
Impact NBIC has awarded 65 Proof of Concept projects, these projects are with the academic partners lists and industry collaborators. We have held three workshop (Biofilm detection, engineering and management), also see publications. The National Biofilms innovation centre is working across disciplines, including: engineering, chemistry, surface and interface science, functional materials, soft matter and non-equilibrium physics, environmental sciences, microbiology, 'omics, bioinformatics, computation and advanced techniques.
Start Year 2018
 
Description National Biofilm Innovation Centre 
Organisation University of Liverpool
Country United Kingdom 
Sector Academic/University 
PI Contribution 1) Sectorial workshops with NBIC Company / Academic Community. This gives the opportunity to form links and possible POC projects to be identified. 2) Holding Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. This gives the opportunity to influence NBIC strategy and funding calls. 3) Identifying NBIC fully or part funded Strategic Research (Defined by Sectorial Workshops and approved by Boards). 4) Providing access to POC calls aligned with NBIC Strategy along with Industrial Partner via Sector or Theme. Competitive transparent Open Process Univ signed up to NBIC with companies willing to sign Project Agreement. Selected using range of criteria within a template case. Support in POC submissions and advice from NBIC engagement officers 5) Facilitating collaborative Access to facilities (eg Diamond / Hartree) (Note Science and Industrial Advisory Boards will have regular review and input into these processes and content of themes etc)
Collaborator Contribution 1) Participation in Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. 2) Application to the NBIC Proof of Concept funding for translational research projects 3) Application to studentships and placements with industrial partners
Impact NBIC has awarded 65 Proof of Concept projects, these projects are with the academic partners lists and industry collaborators. We have held three workshop (Biofilm detection, engineering and management), also see publications. The National Biofilms innovation centre is working across disciplines, including: engineering, chemistry, surface and interface science, functional materials, soft matter and non-equilibrium physics, environmental sciences, microbiology, 'omics, bioinformatics, computation and advanced techniques.
Start Year 2018
 
Description National Biofilm Innovation Centre 
Organisation University of Manchester
Country United Kingdom 
Sector Academic/University 
PI Contribution 1) Sectorial workshops with NBIC Company / Academic Community. This gives the opportunity to form links and possible POC projects to be identified. 2) Holding Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. This gives the opportunity to influence NBIC strategy and funding calls. 3) Identifying NBIC fully or part funded Strategic Research (Defined by Sectorial Workshops and approved by Boards). 4) Providing access to POC calls aligned with NBIC Strategy along with Industrial Partner via Sector or Theme. Competitive transparent Open Process Univ signed up to NBIC with companies willing to sign Project Agreement. Selected using range of criteria within a template case. Support in POC submissions and advice from NBIC engagement officers 5) Facilitating collaborative Access to facilities (eg Diamond / Hartree) (Note Science and Industrial Advisory Boards will have regular review and input into these processes and content of themes etc)
Collaborator Contribution 1) Participation in Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. 2) Application to the NBIC Proof of Concept funding for translational research projects 3) Application to studentships and placements with industrial partners
Impact NBIC has awarded 65 Proof of Concept projects, these projects are with the academic partners lists and industry collaborators. We have held three workshop (Biofilm detection, engineering and management), also see publications. The National Biofilms innovation centre is working across disciplines, including: engineering, chemistry, surface and interface science, functional materials, soft matter and non-equilibrium physics, environmental sciences, microbiology, 'omics, bioinformatics, computation and advanced techniques.
Start Year 2018
 
Description National Biofilm Innovation Centre 
Organisation University of Nottingham
Country United Kingdom 
Sector Academic/University 
PI Contribution 1) Sectorial workshops with NBIC Company / Academic Community. This gives the opportunity to form links and possible POC projects to be identified. 2) Holding Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. This gives the opportunity to influence NBIC strategy and funding calls. 3) Identifying NBIC fully or part funded Strategic Research (Defined by Sectorial Workshops and approved by Boards). 4) Providing access to POC calls aligned with NBIC Strategy along with Industrial Partner via Sector or Theme. Competitive transparent Open Process Univ signed up to NBIC with companies willing to sign Project Agreement. Selected using range of criteria within a template case. Support in POC submissions and advice from NBIC engagement officers 5) Facilitating collaborative Access to facilities (eg Diamond / Hartree) (Note Science and Industrial Advisory Boards will have regular review and input into these processes and content of themes etc)
Collaborator Contribution 1) Participation in Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. 2) Application to the NBIC Proof of Concept funding for translational research projects 3) Application to studentships and placements with industrial partners
Impact NBIC has awarded 65 Proof of Concept projects, these projects are with the academic partners lists and industry collaborators. We have held three workshop (Biofilm detection, engineering and management), also see publications. The National Biofilms innovation centre is working across disciplines, including: engineering, chemistry, surface and interface science, functional materials, soft matter and non-equilibrium physics, environmental sciences, microbiology, 'omics, bioinformatics, computation and advanced techniques.
Start Year 2018
 
Description National Biofilm Innovation Centre 
Organisation University of Oxford
Country United Kingdom 
Sector Academic/University 
PI Contribution 1) Sectorial workshops with NBIC Company / Academic Community. This gives the opportunity to form links and possible POC projects to be identified. 2) Holding Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. This gives the opportunity to influence NBIC strategy and funding calls. 3) Identifying NBIC fully or part funded Strategic Research (Defined by Sectorial Workshops and approved by Boards). 4) Providing access to POC calls aligned with NBIC Strategy along with Industrial Partner via Sector or Theme. Competitive transparent Open Process Univ signed up to NBIC with companies willing to sign Project Agreement. Selected using range of criteria within a template case. Support in POC submissions and advice from NBIC engagement officers 5) Facilitating collaborative Access to facilities (eg Diamond / Hartree) (Note Science and Industrial Advisory Boards will have regular review and input into these processes and content of themes etc)
Collaborator Contribution 1) Participation in Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. 2) Application to the NBIC Proof of Concept funding for translational research projects 3) Application to studentships and placements with industrial partners
Impact NBIC has awarded 65 Proof of Concept projects, these projects are with the academic partners lists and industry collaborators. We have held three workshop (Biofilm detection, engineering and management), also see publications. The National Biofilms innovation centre is working across disciplines, including: engineering, chemistry, surface and interface science, functional materials, soft matter and non-equilibrium physics, environmental sciences, microbiology, 'omics, bioinformatics, computation and advanced techniques.
Start Year 2018
 
Description National Biofilm Innovation Centre 
Organisation University of Portsmouth
Country United Kingdom 
Sector Academic/University 
PI Contribution 1) Sectorial workshops with NBIC Company / Academic Community. This gives the opportunity to form links and possible POC projects to be identified. 2) Holding Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. This gives the opportunity to influence NBIC strategy and funding calls. 3) Identifying NBIC fully or part funded Strategic Research (Defined by Sectorial Workshops and approved by Boards). 4) Providing access to POC calls aligned with NBIC Strategy along with Industrial Partner via Sector or Theme. Competitive transparent Open Process Univ signed up to NBIC with companies willing to sign Project Agreement. Selected using range of criteria within a template case. Support in POC submissions and advice from NBIC engagement officers 5) Facilitating collaborative Access to facilities (eg Diamond / Hartree) (Note Science and Industrial Advisory Boards will have regular review and input into these processes and content of themes etc)
Collaborator Contribution 1) Participation in Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. 2) Application to the NBIC Proof of Concept funding for translational research projects 3) Application to studentships and placements with industrial partners
Impact NBIC has awarded 65 Proof of Concept projects, these projects are with the academic partners lists and industry collaborators. We have held three workshop (Biofilm detection, engineering and management), also see publications. The National Biofilms innovation centre is working across disciplines, including: engineering, chemistry, surface and interface science, functional materials, soft matter and non-equilibrium physics, environmental sciences, microbiology, 'omics, bioinformatics, computation and advanced techniques.
Start Year 2018
 
Description National Biofilm Innovation Centre 
Organisation University of Sheffield
Country United Kingdom 
Sector Academic/University 
PI Contribution 1) Sectorial workshops with NBIC Company / Academic Community. This gives the opportunity to form links and possible POC projects to be identified. 2) Holding Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. This gives the opportunity to influence NBIC strategy and funding calls. 3) Identifying NBIC fully or part funded Strategic Research (Defined by Sectorial Workshops and approved by Boards). 4) Providing access to POC calls aligned with NBIC Strategy along with Industrial Partner via Sector or Theme. Competitive transparent Open Process Univ signed up to NBIC with companies willing to sign Project Agreement. Selected using range of criteria within a template case. Support in POC submissions and advice from NBIC engagement officers 5) Facilitating collaborative Access to facilities (eg Diamond / Hartree) (Note Science and Industrial Advisory Boards will have regular review and input into these processes and content of themes etc)
Collaborator Contribution 1) Participation in Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. 2) Application to the NBIC Proof of Concept funding for translational research projects 3) Application to studentships and placements with industrial partners
Impact NBIC has awarded 65 Proof of Concept projects, these projects are with the academic partners lists and industry collaborators. We have held three workshop (Biofilm detection, engineering and management), also see publications. The National Biofilms innovation centre is working across disciplines, including: engineering, chemistry, surface and interface science, functional materials, soft matter and non-equilibrium physics, environmental sciences, microbiology, 'omics, bioinformatics, computation and advanced techniques.
Start Year 2018
 
Description National Biofilm Innovation Centre 
Organisation University of Southampton
Country United Kingdom 
Sector Academic/University 
PI Contribution 1) Sectorial workshops with NBIC Company / Academic Community. This gives the opportunity to form links and possible POC projects to be identified. 2) Holding Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. This gives the opportunity to influence NBIC strategy and funding calls. 3) Identifying NBIC fully or part funded Strategic Research (Defined by Sectorial Workshops and approved by Boards). 4) Providing access to POC calls aligned with NBIC Strategy along with Industrial Partner via Sector or Theme. Competitive transparent Open Process Univ signed up to NBIC with companies willing to sign Project Agreement. Selected using range of criteria within a template case. Support in POC submissions and advice from NBIC engagement officers 5) Facilitating collaborative Access to facilities (eg Diamond / Hartree) (Note Science and Industrial Advisory Boards will have regular review and input into these processes and content of themes etc)
Collaborator Contribution 1) Participation in Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. 2) Application to the NBIC Proof of Concept funding for translational research projects 3) Application to studentships and placements with industrial partners
Impact NBIC has awarded 65 Proof of Concept projects, these projects are with the academic partners lists and industry collaborators. We have held three workshop (Biofilm detection, engineering and management), also see publications. The National Biofilms innovation centre is working across disciplines, including: engineering, chemistry, surface and interface science, functional materials, soft matter and non-equilibrium physics, environmental sciences, microbiology, 'omics, bioinformatics, computation and advanced techniques.
Start Year 2018
 
Description National Biofilm Innovation Centre 
Organisation University of St Andrews
Country United Kingdom 
Sector Academic/University 
PI Contribution 1) Sectorial workshops with NBIC Company / Academic Community. This gives the opportunity to form links and possible POC projects to be identified. 2) Holding Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. This gives the opportunity to influence NBIC strategy and funding calls. 3) Identifying NBIC fully or part funded Strategic Research (Defined by Sectorial Workshops and approved by Boards). 4) Providing access to POC calls aligned with NBIC Strategy along with Industrial Partner via Sector or Theme. Competitive transparent Open Process Univ signed up to NBIC with companies willing to sign Project Agreement. Selected using range of criteria within a template case. Support in POC submissions and advice from NBIC engagement officers 5) Facilitating collaborative Access to facilities (eg Diamond / Hartree) (Note Science and Industrial Advisory Boards will have regular review and input into these processes and content of themes etc)
Collaborator Contribution 1) Participation in Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. 2) Application to the NBIC Proof of Concept funding for translational research projects 3) Application to studentships and placements with industrial partners
Impact NBIC has awarded 65 Proof of Concept projects, these projects are with the academic partners lists and industry collaborators. We have held three workshop (Biofilm detection, engineering and management), also see publications. The National Biofilms innovation centre is working across disciplines, including: engineering, chemistry, surface and interface science, functional materials, soft matter and non-equilibrium physics, environmental sciences, microbiology, 'omics, bioinformatics, computation and advanced techniques.
Start Year 2018
 
Description National Biofilm Innovation Centre 
Organisation University of Strathclyde
Country United Kingdom 
Sector Academic/University 
PI Contribution 1) Sectorial workshops with NBIC Company / Academic Community. This gives the opportunity to form links and possible POC projects to be identified. 2) Holding Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. This gives the opportunity to influence NBIC strategy and funding calls. 3) Identifying NBIC fully or part funded Strategic Research (Defined by Sectorial Workshops and approved by Boards). 4) Providing access to POC calls aligned with NBIC Strategy along with Industrial Partner via Sector or Theme. Competitive transparent Open Process Univ signed up to NBIC with companies willing to sign Project Agreement. Selected using range of criteria within a template case. Support in POC submissions and advice from NBIC engagement officers 5) Facilitating collaborative Access to facilities (eg Diamond / Hartree) (Note Science and Industrial Advisory Boards will have regular review and input into these processes and content of themes etc)
Collaborator Contribution 1) Participation in Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. 2) Application to the NBIC Proof of Concept funding for translational research projects 3) Application to studentships and placements with industrial partners
Impact NBIC has awarded 65 Proof of Concept projects, these projects are with the academic partners lists and industry collaborators. We have held three workshop (Biofilm detection, engineering and management), also see publications. The National Biofilms innovation centre is working across disciplines, including: engineering, chemistry, surface and interface science, functional materials, soft matter and non-equilibrium physics, environmental sciences, microbiology, 'omics, bioinformatics, computation and advanced techniques.
Start Year 2018
 
Description National Biofilm Innovation Centre 
Organisation University of Surrey
Country United Kingdom 
Sector Academic/University 
PI Contribution 1) Sectorial workshops with NBIC Company / Academic Community. This gives the opportunity to form links and possible POC projects to be identified. 2) Holding Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. This gives the opportunity to influence NBIC strategy and funding calls. 3) Identifying NBIC fully or part funded Strategic Research (Defined by Sectorial Workshops and approved by Boards). 4) Providing access to POC calls aligned with NBIC Strategy along with Industrial Partner via Sector or Theme. Competitive transparent Open Process Univ signed up to NBIC with companies willing to sign Project Agreement. Selected using range of criteria within a template case. Support in POC submissions and advice from NBIC engagement officers 5) Facilitating collaborative Access to facilities (eg Diamond / Hartree) (Note Science and Industrial Advisory Boards will have regular review and input into these processes and content of themes etc)
Collaborator Contribution 1) Participation in Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. 2) Application to the NBIC Proof of Concept funding for translational research projects 3) Application to studentships and placements with industrial partners
Impact NBIC has awarded 65 Proof of Concept projects, these projects are with the academic partners lists and industry collaborators. We have held three workshop (Biofilm detection, engineering and management), also see publications. The National Biofilms innovation centre is working across disciplines, including: engineering, chemistry, surface and interface science, functional materials, soft matter and non-equilibrium physics, environmental sciences, microbiology, 'omics, bioinformatics, computation and advanced techniques.
Start Year 2018
 
Description National Biofilm Innovation Centre 
Organisation University of Sussex
Country United Kingdom 
Sector Academic/University 
PI Contribution 1) Sectorial workshops with NBIC Company / Academic Community. This gives the opportunity to form links and possible POC projects to be identified. 2) Holding Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. This gives the opportunity to influence NBIC strategy and funding calls. 3) Identifying NBIC fully or part funded Strategic Research (Defined by Sectorial Workshops and approved by Boards). 4) Providing access to POC calls aligned with NBIC Strategy along with Industrial Partner via Sector or Theme. Competitive transparent Open Process Univ signed up to NBIC with companies willing to sign Project Agreement. Selected using range of criteria within a template case. Support in POC submissions and advice from NBIC engagement officers 5) Facilitating collaborative Access to facilities (eg Diamond / Hartree) (Note Science and Industrial Advisory Boards will have regular review and input into these processes and content of themes etc)
Collaborator Contribution 1) Participation in Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. 2) Application to the NBIC Proof of Concept funding for translational research projects 3) Application to studentships and placements with industrial partners
Impact NBIC has awarded 65 Proof of Concept projects, these projects are with the academic partners lists and industry collaborators. We have held three workshop (Biofilm detection, engineering and management), also see publications. The National Biofilms innovation centre is working across disciplines, including: engineering, chemistry, surface and interface science, functional materials, soft matter and non-equilibrium physics, environmental sciences, microbiology, 'omics, bioinformatics, computation and advanced techniques.
Start Year 2018
 
Description National Biofilm Innovation Centre 
Organisation University of Warwick
Country United Kingdom 
Sector Academic/University 
PI Contribution 1) Sectorial workshops with NBIC Company / Academic Community. This gives the opportunity to form links and possible POC projects to be identified. 2) Holding Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. This gives the opportunity to influence NBIC strategy and funding calls. 3) Identifying NBIC fully or part funded Strategic Research (Defined by Sectorial Workshops and approved by Boards). 4) Providing access to POC calls aligned with NBIC Strategy along with Industrial Partner via Sector or Theme. Competitive transparent Open Process Univ signed up to NBIC with companies willing to sign Project Agreement. Selected using range of criteria within a template case. Support in POC submissions and advice from NBIC engagement officers 5) Facilitating collaborative Access to facilities (eg Diamond / Hartree) (Note Science and Industrial Advisory Boards will have regular review and input into these processes and content of themes etc)
Collaborator Contribution 1) Participation in Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. 2) Application to the NBIC Proof of Concept funding for translational research projects 3) Application to studentships and placements with industrial partners
Impact NBIC has awarded 65 Proof of Concept projects, these projects are with the academic partners lists and industry collaborators. We have held three workshop (Biofilm detection, engineering and management), also see publications. The National Biofilms innovation centre is working across disciplines, including: engineering, chemistry, surface and interface science, functional materials, soft matter and non-equilibrium physics, environmental sciences, microbiology, 'omics, bioinformatics, computation and advanced techniques.
Start Year 2018
 
Description National Biofilm Innovation Centre 
Organisation University of York
Country United Kingdom 
Sector Academic/University 
PI Contribution 1) Sectorial workshops with NBIC Company / Academic Community. This gives the opportunity to form links and possible POC projects to be identified. 2) Holding Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. This gives the opportunity to influence NBIC strategy and funding calls. 3) Identifying NBIC fully or part funded Strategic Research (Defined by Sectorial Workshops and approved by Boards). 4) Providing access to POC calls aligned with NBIC Strategy along with Industrial Partner via Sector or Theme. Competitive transparent Open Process Univ signed up to NBIC with companies willing to sign Project Agreement. Selected using range of criteria within a template case. Support in POC submissions and advice from NBIC engagement officers 5) Facilitating collaborative Access to facilities (eg Diamond / Hartree) (Note Science and Industrial Advisory Boards will have regular review and input into these processes and content of themes etc)
Collaborator Contribution 1) Participation in Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. 2) Application to the NBIC Proof of Concept funding for translational research projects 3) Application to studentships and placements with industrial partners
Impact NBIC has awarded 65 Proof of Concept projects, these projects are with the academic partners lists and industry collaborators. We have held three workshop (Biofilm detection, engineering and management), also see publications. The National Biofilms innovation centre is working across disciplines, including: engineering, chemistry, surface and interface science, functional materials, soft matter and non-equilibrium physics, environmental sciences, microbiology, 'omics, bioinformatics, computation and advanced techniques.
Start Year 2018
 
Description National Biofilm Innovation Centre 
Organisation University of the Highlands and Islands
Country United Kingdom 
Sector Academic/University 
PI Contribution 1) Sectorial workshops with NBIC Company / Academic Community. This gives the opportunity to form links and possible POC projects to be identified. 2) Holding Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. This gives the opportunity to influence NBIC strategy and funding calls. 3) Identifying NBIC fully or part funded Strategic Research (Defined by Sectorial Workshops and approved by Boards). 4) Providing access to POC calls aligned with NBIC Strategy along with Industrial Partner via Sector or Theme. Competitive transparent Open Process Univ signed up to NBIC with companies willing to sign Project Agreement. Selected using range of criteria within a template case. Support in POC submissions and advice from NBIC engagement officers 5) Facilitating collaborative Access to facilities (eg Diamond / Hartree) (Note Science and Industrial Advisory Boards will have regular review and input into these processes and content of themes etc)
Collaborator Contribution 1) Participation in Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. 2) Application to the NBIC Proof of Concept funding for translational research projects 3) Application to studentships and placements with industrial partners
Impact NBIC has awarded 65 Proof of Concept projects, these projects are with the academic partners lists and industry collaborators. We have held three workshop (Biofilm detection, engineering and management), also see publications. The National Biofilms innovation centre is working across disciplines, including: engineering, chemistry, surface and interface science, functional materials, soft matter and non-equilibrium physics, environmental sciences, microbiology, 'omics, bioinformatics, computation and advanced techniques.
Start Year 2018
 
Description National Biofilm Innovation Centre 
Organisation University of the West of England
Country United Kingdom 
Sector Academic/University 
PI Contribution 1) Sectorial workshops with NBIC Company / Academic Community. This gives the opportunity to form links and possible POC projects to be identified. 2) Holding Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. This gives the opportunity to influence NBIC strategy and funding calls. 3) Identifying NBIC fully or part funded Strategic Research (Defined by Sectorial Workshops and approved by Boards). 4) Providing access to POC calls aligned with NBIC Strategy along with Industrial Partner via Sector or Theme. Competitive transparent Open Process Univ signed up to NBIC with companies willing to sign Project Agreement. Selected using range of criteria within a template case. Support in POC submissions and advice from NBIC engagement officers 5) Facilitating collaborative Access to facilities (eg Diamond / Hartree) (Note Science and Industrial Advisory Boards will have regular review and input into these processes and content of themes etc)
Collaborator Contribution 1) Participation in Scientific NBIC Forum around key themes to establish consensus areas and key unanswered questions. 2) Application to the NBIC Proof of Concept funding for translational research projects 3) Application to studentships and placements with industrial partners
Impact NBIC has awarded 65 Proof of Concept projects, these projects are with the academic partners lists and industry collaborators. We have held three workshop (Biofilm detection, engineering and management), also see publications. The National Biofilms innovation centre is working across disciplines, including: engineering, chemistry, surface and interface science, functional materials, soft matter and non-equilibrium physics, environmental sciences, microbiology, 'omics, bioinformatics, computation and advanced techniques.
Start Year 2018
 
Description PhD Exploring innovative regenerative scaffolds for simultaneous bacteria/host monitoring and healing promotion (Mat Hardman) 
Organisation Neotherix Ltd
Country United Kingdom 
Sector Private 
PI Contribution Training, supervision and funding for this PhD.
Collaborator Contribution Training, supervision and funding for this PhD.
Impact None yet.
Start Year 2022
 
Description PhD Exploring innovative regenerative scaffolds for simultaneous bacteria/host monitoring and healing promotion (Mat Hardman) 
Organisation University of Hull
Country United Kingdom 
Sector Academic/University 
PI Contribution Training, supervision and funding for this PhD.
Collaborator Contribution Training, supervision and funding for this PhD.
Impact None yet.
Start Year 2022
 
Description Studies in Solid Formulation Stability 
Organisation GlaxoSmithKline (GSK)
Country Global 
Sector Private 
PI Contribution Due to the work conducted during my EngD studies in combination with the success of this project (NBIC_FTMA_P_19_2_38), GSK and UCL signed an Exchange Knowledge Agreement and funded a 6 month study on the stability of solid formulations. The study was conducted by me under supervision of Ivan Parkin and employed a spectroscopic techniques (H- NMR, IR, UV/Vis) and scanning electron microscopy. This study can help the development of stronger formulations by giving insights on the increase of its reactive oxygen species output.
Collaborator Contribution Funding of study.
Impact None as yet.
Start Year 2022
 
Description The University of Sheffield - Kraton Collaboration (Andrew Parnell) 
Organisation University of Sheffield
Department Sheffield Biorepository
Country United Kingdom 
Sector Academic/University 
PI Contribution We have used the existing commercially available block copolymers to show how important stiffness is on producing materials that have surface antibacterial properties. These are stiff but also have rubbery behaviour making them suitable for a whole host of technological applications.
Collaborator Contribution Kraton have and will continue to provide an assortment of cutting edge block copolymers. These are bcp's with varying chain architecture. These are currently being synthesised in their pilot plant in Houston. In principle they have the capability to made at scale and are low cost due to the feedstock material. They also have been used previously in some medical implants, as such the routes to gaining clearance will be easier.
Impact A draft of a patent is currently being processed by my technology transfer office.
Start Year 2021
 
Title Antiviral filters for face masks and air ventilation systems to prevent coronavirus transmission (Faradin Mirkhalaf) 
Description We have developed a new technology for the modification of filters in order to capture and kill viruses and bacteria from the air flow. The modified filters were used in face masks and tested on coronavirus. After testing, the filters showed 100% efficient to remove and kill corona viruses from the air flow. The face masks have been prototyped and are in the market (see: nanoxx.co.uk).The application of this technology for air ventilation systems is under development and we are seeking industrial/investor partners to complete this mission. 
Type Preventative Intervention - Physical/Biological risk modification
Current Stage Of Development Small-scale adoption
Year Development Stage Completed 2021
Development Status Actively seeking support
Impact We are using a similar technology to modify metal and textiles to induce antibiofilm properties within our NBIC FTMA3 projects. 
URL http://nanoxx.co.uk
 
Title Nanoparticles (Eden Mannix-Fisher) 
Description Copper nanoparticles created by Pharm2Farm are being tested for their antimicrobial efficacy when mixed into paint and coated onto the surface of paint. The product can have many different applications both within and outside of medicine if successful. 
Type Products with applications outside of medicine
Current Stage Of Development Initial development
Year Development Stage Completed 2022
Development Status Under active development/distribution
Impact Should this antimicrobial work, the data generated from this secondment on the antimicrobial efficacy may generate income for Pharm2Farm and the customer AksoNobel. It may also be used to decrease the incidence of disease through utilisation as a coating on door handles, walls, beds etc. within a hospital setting. 
 
Description Exploring Novel Anti-biofilm Technology Use in New Areas (NBIC Case Study) 
Form Of Engagement Activity Engagement focused website, blog or social media channel
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Highlight the success of an NBIC funded project.
Year(s) Of Engagement Activity 2021
URL https://www.biofilms.ac.uk/anti-biofilm-technology/
 
Description Interview for Radio 5 live (Eleanor Jameson) 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact I gave an interview on Naga Munchetty's lunch time national radio show.
Year(s) Of Engagement Activity 2021
 
Description NBIC Secure 180K from BBSRC for FTMA3 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact News article on NBIC website.
Year(s) Of Engagement Activity 2021
URL https://www.biofilms.ac.uk/nbic-ftma3/
 
Description Poster at the 32nd European Congress of Clinical Microbiology & Infectious Diseases (Mohamed El Mohtadi) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact I have presented my fellowship-related research findings at the 32nd European Congress of Clinical Microbiology & Infectious Diseases (Lisbon, Portugal on 23 - 26 April 2022). ECCMID is labelled as the world's premier Clinical Microbiology & Infectious Diseases event, which was attended by an audience of over 14,000 colleagues from National and International institutions.
Year(s) Of Engagement Activity 2022
 
Description Poster presentation at the 32nd European Congress of Clinical Microbiology & Infectious Diseases 2022 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact I have presented my fellowship-related research findings at the 32nd European Congress of Clinical Microbiology & Infectious Diseases, which took place in Lisbon, Portugal on 23 - 26 April 2022. ECCMID is labelled as the world's premier Clinical Microbiology & Infectious Diseases event, which was attended by an audience of over 14,000 colleagues from National and International institutions.
Year(s) Of Engagement Activity 2022
 
Description Secrete Science of Sewage (Eleanor Jameson) 
Form Of Engagement Activity A broadcast e.g. TV/radio/film/podcast (other than news/press)
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Media (as a channel to the public)
Results and Impact My research was discussed on a BBC horizon documentary. I discussed my research and implications with the presenter and demonstrated my bacteriophages.
Year(s) Of Engagement Activity 2020
URL https://www.bbc.co.uk/iplayer/episode/m000t8zl/the-secret-science-of-sewage
 
Description Transforming 16S Sequencing (NBIC case study) 
Form Of Engagement Activity Engagement focused website, blog or social media channel
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Highlight the success of an NBIC funded project.
Year(s) Of Engagement Activity 2020
URL https://www.biofilms.ac.uk/transforming-16s-sequencing/