UK-Brazil International Partnering Award: Development of novel strategies to address plant-microbes interactions in planta

Lead Research Organisation: University of Warwick
Department Name: School of Life Sciences

Abstract

Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.

Publications

10 25 50
 
Description Microbiome strategic road map
Geographic Reach Europe 
Policy Influence Type Participation in a guidance/advisory committee
URL https://iuk.ktn-uk.org/wp-content/uploads/2021/02/Microbiome_Strategic_Roadmap_FINAL.pdf
 
Description Development of novel strategies to address plant-microbes interactions in planta 
Organisation Universidade de São Paulo
Country Brazil 
Sector Academic/University 
PI Contribution This is a new UK-Brazil international partnership to study plant-microbe interactions involved in symbiosis and regulation of plant growth and development influenced by the circadian clock between the University of Warwick and the University of São Paulo, Brazil. It brings together recent work by Isabelle Carré and Miriam Gifford at the University of Warwick uncovered the impact of the circadian clock on symbiotic interaction between the legume Medicago truncatula and nitrogen-fixing rhizobia, leading to a current BBSRC-funded project (BB/T015357/1; Achom et al. 2021 Journal of Experimental Botany).
Collaborator Contribution It also links the work of Isabelle Carré and Gary Bending at the University of Warwick who discovered that plant circadian rhythms influence the composition of the rhizosphere microbiome (Newman et al., Under Revision). Joining forces with Carlos Hotta and Marie-Anne Van Sluys at the University of São Paulo, Brazil, will enable us to access expertise on circadian rhythms and endophytes in the important crop of sugarcane (Dantas et al. 2021 New Phytologist). It will also bring enable use of fluorescent bacterial cell lines, which will be valuable tools for validation of our experimental approaches.
Impact Updated March 2023: (1) Researcher exchange from University of São Paulo -> University of Warwick (November 2022): researchers developed bacterial GFP-lines to track endophyte colonisation of Setaria viridis at Warwick and learnt methods for cell dissociation from tissue, and cell sorting. (2) Researcher exchange from University of Warwick -> University of São Paulo (February 2023): researchers developed methods to separate and sequence endophytes, using a range of cell strainers and sequence of dissociation methods. Next we plan a bilateral workshop to plan next steps.
Start Year 2022