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AI-Optimised Fermentation for Sustainable Protein Production from Food Side Streams

Lead Research Organisation: University of Leeds
Department Name: School of Food Science and Nutrition

Abstract

This international collaboration will develop AI methods for optimising fermentation processes that use food side streams as substrates to produce sustainable proteins for human consumption. It will be led by the University of Leeds (UoL) and the Commonwealth Scientific and Industrial Research Organisation (CSIRO) and includes four industrial partners from the agri-food sector.

Current alternative protein production often uses high-sugar substrates. This project aims to utilise food side streams as the fermentation substrate to increase sustainability and economic viability of protein production. Given the variability between different food side streams, AI will be used to aid optimisation of fermentation parameters like solids and moisture content, pre-treatment methods, pH levels, yeast strains, and nutrient supplementation.

Approximately one-third of food produced gets wasted. The project addresses this waste, contributes to creating a circular economy, while simultaneously producing protein to enhance food security for a growing world population. The project is aligned with two of the competition's themes on AI in sustainable agriculture and food and AI to advance manufacturing and clean growth.

Each institution has complementary capabilities where the University of Leeds team offers experience in experimental data collection and preliminary model development and CSIRO brings expertise in scaling up these processes to ensure they have a broader, real-world impact. The partnership also addresses regional variations in food side streams enabling the development of adaptable models with greater generalisability. The projects activities are organised into five Work Packages (WPs):

WP1 (UoL): Experimental data collection: Collection of data from fermentation processes using food side streams (short shelf-life soft fruit and Jerusalem artichokes) as substrates to produce protein.
WP2 (UoL & CSIRO): Single side stream models: Leveraging the data from WP1, development of AI models to predict fermentation products (e.g., microbial dynamics, yield, and protein concentration) based on the side stream composition and fermentation parameters.
WP3 (UoL): Adaptive modelling techniques: Using the data from WP1, development of transfer learning and Bayesian optimisation methodologies to significantly reduce the data collection burden for new side streams or equipment.
WP4 (CSIRO): Scale up and out: The methodologies from WP3 will guide data collection from scaled-up fermentation trials at CSIRO using new food side streams aiming to maximise sustainability and economics.
WP5 (UoL & CSIRO): Partnership and impact: Focusing on disseminating findings to the broader scientific community, sharing data, code, models, methodologies, and academic papers; and conducting partnership activities including visits, workshops, and training sessions.
The outcome of the partnership between UoL and CSIRO will be enhanced AI capabilities at each organisation, the career development of project team members and adaptable AI models that can be used by beneficiaries world-wide to efficiently optimise fermentation processes and assess the potential of new food side streams.

Publications

10 25 50
 
Description AI Platform for Sustainable Protein from Agri-Food Waste
Amount $1,998,000 (USD)
Funding ID G-2025-00873 
Organisation Bezos Earth Fund 
Sector Charity/Non Profit
Country United States
Start 01/2026 
End 12/2027
 
Description Fortyeast
Amount £500,000 (GBP)
Funding ID 133100 
Organisation Innovate UK 
Sector Public
Country United Kingdom
Start 12/2024 
End 11/2026
 
Description Exploitation of yeast biodiversity 
Organisation Commonwealth Scientific and Industrial Research Organisation
Country Australia 
Sector Public 
PI Contribution Dr Carol Hartley, Senior Research Scientist & Engineering Biology Team Leader, CSIRO I shared my work with Carol and we saw the bridges and start exchanging ideas. Carol then kindly provide my group with several yeast strains that they have good results and I have been using these in my work but also on yeast biomass from waste for the present project.
Collaborator Contribution above
Impact yet to come
Start Year 2024
 
Description Fermentation benefits 
Organisation University of Melbourne
Country Australia 
Sector Academic/University 
PI Contribution Prof Kate Howell During our visit to Australia we had the opportunity to visit some universities including University of Melbourne and talk with different researchers and professionals. During these talks we could see ideas we could explore together and that is what happened with Prof Kate Howell. she show interest in having PhD students together and I propose the possibility of her entering in the consortia I am elaborating for a Marie currie DN. We just had the first couple of talks. We want to apply for PHD exchange and prepare a Marie curie Doctoral Network for the next call (fall 2025)
Collaborator Contribution above
Impact we just started
Start Year 2025
 
Description Conversion of under-resourced crops, bio-waste, and other biomass into high value products using a yeast fermentation 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Presentation to the Association of Applied Biologists for their crops conference Aberystwyth, September 2024.
Year(s) Of Engagement Activity 2024
 
Description Modelling in Food: Machine Learning, Life Cycle Assessment, and Techno-economic Analysis 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Postgraduate students
Results and Impact Project work delivered to students studying the "Digital Tools" module in Food Science and Nutrition at the University of Leeds. Led to increased interest in PhD programs and new directions for the module (e.g., coding, AI).
Year(s) Of Engagement Activity 2025
 
Description World submit on Food Science and Nutrition 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact World submit on Food Science and Nutrition, October 2024, Bern Switzerland- invited talk
Year(s) Of Engagement Activity 2024