The Development of High Throughput Automated Microscopy to Investigate the Developmental Genetics of Model Organisms
Lead Research Organisation:
University of Sheffield
Department Name: Biomedical Science
Abstract
Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.
Technical Summary
Developmental biology and genetics research groups in Sheffield are engaged in a broad range of research projects in the biomolecular sciences area at an internationally competitive level, and this application is a request for a state-of-the-art, high throughout, real-time epifluoresence microscope system to support this work. Projects include studies into the fundamental biology of human embryonic stem cells, genetic stability of human embryonic stem cells, lineage segregation in stem cells and organisms. All of these projects will benefit from the range of new experiments which can be performed by the instrument requested and which are not currently possible using the microscopy systems currently available in the faculty of medicine.
Publications
Avery S
(2010)
The role of SMAD4 in human embryonic stem cell self-renewal and stem cell fate.
in Stem cells (Dayton, Ohio)
Barbaric I
(2011)
High-content screening for chemical modulators of embryonal carcinoma cell differentiation and survival.
in Journal of biomolecular screening
Barbaric I
(2011)
Pinacidil enhances survival of cryopreserved human embryonic stem cells.
in Cryobiology
Barbaric I
(2010)
Novel regulators of stem cell fates identified by a multivariate phenotype screen of small compounds on human embryonic stem cell colonies.
in Stem cell research
Harrison NJ
(2009)
CD30 expression reveals that culture adaptation of human embryonic stem cells can occur through differing routes.
in Stem cells (Dayton, Ohio)
Mohanty ST
(2012)
A small molecule modulator of prion protein increases human mesenchymal stem cell lifespan, ex vivo expansion, and engraftment to bone marrow in NOD/SCID mice.
in Stem cells (Dayton, Ohio)
Na J
(2010)
Inhibition of ERK1/2 prevents neural and mesendodermal differentiation and promotes human embryonic stem cell self-renewal.
in Stem cell research
Neil Harrison (Co-Author)
(2009)
CD30 expression reveals that culture adaptation of human embryonic stem cells can occur through differing routes.
in Stem Cells
Sood A
(2011)
Signalling of DNA damage and cytokines across cell barriers exposed to nanoparticles depends on barrier thickness
in Nature Nanotechnology
Wright A
(2011)
Mapping the stem cell state: eight novel human embryonic stem and embryonal carcinoma cell antibodies.
in International journal of andrology
| Description | We have used this equipment to facilitate experiments to explore the mechanisms that control the growth and differentiation of human embryonic stem cells |
| Exploitation Route | High content clonogenic assays are becoming widespread for the study of the biology underlying pluripotent stem cells. |
| Sectors | Pharmaceuticals and Medical Biotechnology |
| Description | Not applicable - equipment grant |
| First Year Of Impact | 2005 |
| Sector | Pharmaceuticals and Medical Biotechnology |
| Impact Types | Economic |