Imaging of 3D Engineered Tissues
Lead Research Organisation:
University of Sheffield
Department Name: Materials Science and Engineering
Abstract
Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.
Technical Summary
The current proposal outlines research themes on tissue engineering at Sheffield University where a laser scanning confocal microscope with META multichannel confocal detection is requested (with the potential for multiphoton upgrade in the future). The model is for a confocal system to be located in the new interdisciplinary Kroto Research Institute at Sheffield University. This facility will have a suite of GMP accredited clean rooms to enable tissue engineered construct formation under sterile conditions for clinical use. Multidisciplinary research laboratories located immediately next to the clean rooms will support core research and further development of reconstructed tissues. Location of an on-site confocal microscope in the research laboratories will therefore enable the in-depth study of cells in synthetic scaffolds across a number of research programmes. Confocal microscopy is a leading-edge technology and a powerful research tool, enabling the capture of unrivalled high resolution of cells within 3 dimensional constructs as well as in-depth penetration of specimens tagged with very specific fluorophores. This leading edge technology is now becoming a method of choice for the dynamic imaging of cells, tissues and biological systems not otherwise possible by other optical systems. Such a technology is essential for driving the research of our team, understanding a range of interconnected problems spanning the life sciences-engineering interface. Each member of our team has programmes that rely heavily on the need for high resolution microscopy which presently is only partially met by existing fluorescence systems. META confocal technology advances the quality of imaging and flexibility of analysis by such a high degree over standard fluorescence that it is now the new benchmark from visualising cells in vitro or in 3 dimension structures in vivo. It therefore has wide applicability in biology, chemistry and engineering and this is represented by our team of applicants. A central imaging facility will also be essential for maintaining research of an internationally competitive standard and will act as a potent catalyst for attracting high quality collaborations with investigators in the UK and abroad.
Publications
Alépée N
(2014)
State-of-the-art of 3D cultures (organs-on-a-chip) in safety testing and pathophysiology
in ALTEX
Baggaley E
(2014)
Dinuclear ruthenium(II) complexes as two-photon, time-resolved emission microscopy probes for cellular DNA.
in Angewandte Chemie (International ed. in English)
Bell J
(2011)
The use of plasma polymerised acrylic acid and maleic anhydride for neuronal cell engineering
in European Cells and Materials
Blackwood K
(2008)
Long term degradation study of electrospun scaffolds for soft tissue reconstruction
in 8th World Biomaterials Congress 2008
Botchway SW
(2008)
Time-resolved and two-photon emission imaging microscopy of live cells with inert platinum complexes.
in Proceedings of the National Academy of Sciences of the United States of America
Bryan M
(2010)
Erratum: "The effect of trapping superparamagnetic beads on domain wall motion" [Appl. Phys. Lett. 96, 192503 (2010)]
in Applied Physics Letters
Cantòn I
(2010)
Development of a 3D human in vitro skin co-culture model for detecting irritants in real-time.
in Biotechnology and bioengineering
Cantón I
(2007)
Real-time detection of stress in 3D tissue-engineered constructs using NF-kappaB activation in transiently transfected human dermal fibroblast cells.
in Tissue engineering
Cantón I
(2008)
3D tissue engineered constructs for real time detection of stress via NF-?B activation in transiently transfected human dermal fibroblasts
in 8th World Biomaterials Congress 2008
Charnley M
(2009)
Generation of Bioactive Materials with Rapid Self-Assembling Resorcinarene-Peptides
in Advanced Materials
Charnley M
(2007)
Evaluation of anti-inflammatory and antimicrobial resorcinarene-peptides for biomaterial modification
in European Cells and Materials
Charnley M
(2007)
Evaluation of anti-inflammatory and antimicrobial resorcinarene-peptides for biomaterial modification
in European Cells and Materials
Charnley M
(2006)
Evaluation of anti-inflammatory calixarene-peptides for biomaterial modification
in European Cells and Materials
Chau DY
(2013)
The development of a 3D immunocompetent model of human skin.
in Biofabrication
Chunthapong J
(2011)
Development of a tissue-engineered skin model for detecting irritants
in European Cells and Materials
Chunthapong J
(2008)
A 3D Skin tissue-engineered model for inflammatory and toxicity testing
in European Cells and Materials
Daud MF
(2012)
An aligned 3D neuronal-glial co-culture model for peripheral nerve studies.
in Biomaterials
Eves PC
(2006)
alpha-Melanocyte stimulating hormone, inflammation and human melanoma.
in Peptides
Filova E
(2008)
Mesenchymal stems cells and endothelial cells on micropatterned surfaces prepared by plasma polymerisation
in 8th World Biomaterials Congress 2008
Filová E
(2009)
Regionally-selective cell colonization of micropatterned surfaces prepared by plasma polymerization of acrylic acid and 1,7-octadiene.
in Physiological research
Hopper AP
(2014)
Amine functionalized nanodiamond promotes cellular adhesion, proliferation and neurite outgrowth.
in Biomedical materials (Bristol, England)
Hopper AP
(2016)
Photochemically modified diamond-like carbon surfaces for neural interfaces.
in Materials science & engineering. C, Materials for biological applications
Kaewkhaw R
(2011)
Adipose-derived stem cells (ASCs) for peripheral nerve repair
in European Cells and Materials
Kaewkhaw R
(2009)
Adipose-derived stem cells (ASCs) for peripheral nerve repair
in European Cells and Materials
Kaewkhaw R
(2012)
Integrated culture and purification of rat Schwann cells from freshly isolated adult tissue
in Nature Protocols
Kaewkhaw R
(2011)
Adipose-derived stem cells for peripheral nerve repair
in European Cells and Materials
Lizarraga-Valderrama L
(2015)
Nerve tissue engineering using blends of poly(3-hydroxyalkanoates) for peripheral nerve regeneration
in Engineering in Life Sciences
Melissinaki V
(2011)
Direct laser writing of 3D scaffolds for neural tissue engineering applications.
in Biofabrication
Morris H
(2010)
Mechanisms of fluid-flow-induced matrix production in bone tissue engineering
in Proceedings of the Institution of Mechanical Engineers, Part H: Journal of Engineering in Medicine
Murray-Dunning C
(2011)
Three-dimensional alignment of schwann cells using hydrolysable microfiber scaffolds: strategies for peripheral nerve repair.
in Methods in molecular biology (Clifton, N.J.)
Murray-Dunning C
(2008)
Peripheral nerve engineering using aligned polymer microfibres
in European Cells and Materials
Murray-Dunning C
(2009)
The use of aligned polymer microfibres in peripheral nerve engineering
in European Cells and Materials
Murray-Dunning C
(2008)
Use of aligned polymer microfibres for peripheral nerve repair
in 8th World Biomaterials Congress 2008
Pateman CJ
(2015)
Nerve guides manufactured from photocurable polymers to aid peripheral nerve repair.
in Biomaterials
Paviolo C
(2013)
Laser exposure of gold nanorods can increase neuronal cell outgrowth
in Biotechnology and Bioengineering
Paviolo C
(2014)
Laser exposure of gold nanorods can induce intracellular calcium transients.
in Journal of biophotonics
Plenderleith RA
(2015)
Arginine-glycine-aspartic acid functional branched semi-interpenetrating hydrogels.
in Soft matter
Sun T
(2007)
Investigation of fibroblast and keratinocyte cell-scaffold interactions using a novel 3D cell culture system.
in Journal of materials science. Materials in medicine
Sun T
(2008)
Development of a bioreactor for evaluating novel nerve conduits.
in Biotechnology and bioengineering
Zilic L
(2015)
An anatomical study of porcine peripheral nerve and its potential use in nerve tissue engineering.
in Journal of anatomy
| Description | The grant allowed the purchase of a versatile imaging system for conducting work on 3D in vitro cells/tissues. The major outcome has been the discovery of several methods for imaging a breadth of cells and scaffolds in 3D as a alternative to using animals. |
| Exploitation Route | The grant has supported a tremendous breadth of outputs - not just those I am personally associated with (immediate outputs herein), but those of many other groups. These outputs have been documented on the webpage cited above and here - (https://www.sheffield.ac.uk/kroto/confocal). These findings may be taken forward by devising new methods for further reducing animal research, new methods for SMEs to develop new implants, through to new bioreactors for such methods. |
| Sectors | Healthcare |
| URL | https://www.sheffield.ac.uk/kroto/confocal |
| Description | This was an equipment grant for imaging 3D engineered tissues. It has led to a breadth of research represented in several outputs. Parallel findings have arisen including: 1. Design of commercial bioreactors in collaboration with SMEs 2. Design of methods for orthopaedic implants in collaboration with SMEs using 3D in vitro methods. |
| First Year Of Impact | 2014 |
| Sector | Healthcare |
| Impact Types | Economic |