Development and analysis of technologies for measurement of protein interactions in living cells
Lead Research Organisation:
University of Liverpool
Department Name: Sch of Biological Sciences
Abstract
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People |
ORCID iD |
| Michael White (Principal Investigator) |
Publications
Awais M
(2011)
Illuminating intracellular signaling and molecules for single cell analysis.
in Molecular bioSystems
Spiller D
(2010)
Measurement of single-cell dynamics
in Nature
| Description | Development of new spit luciferase tools Advice in FRET experiments Trained visiting scientists in FCS and live cell imaging Exchanged expertise including systems biology methods. |
| Exploitation Route | These collaborations are continuing |
| Sectors | Education,Healthcare,Manufacturing, including Industrial Biotechology |
| Description | This was a collaborative grant with Japan. It provided the basis for an ongoing strong collaboration. This covered luminescence, fluorescent proteins and systems biology. It has helped to develop many underpinning tools. Two Japanese students have visitied Manchester since the grant ended and we have visitied Japan twice. On the most recent visit we were able to visit an expert FCS lab through our Japanese contacts. One of our postdocs has visited Japan since the end of the grant . A further postdoc in 2016 obtained BBSRC funds to visit Japan through a link initiated through contacts made during this grant. |
| First Year Of Impact | 2011 |
| Sector | Education,Healthcare,Pharmaceuticals and Medical Biotechnology |
| Impact Types | Cultural,Societal |
| Description | An upright confocal microscope for multidisciplinary research |
| Amount | £282,781 (GBP) |
| Funding ID | BB/R014361/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 05/2018 |
| End | 04/2019 |
| Description | Carl Zeiss |
| Organisation | Carl Zeiss AG |
| Country | Germany |
| Sector | Private |
| PI Contribution | We have advised Zeiss on trends in bioimaging since 1996. We have provided new data and tested prototype equipment. We have spoken at Zeiss organised meetings. We have given them an opportunity to display Zeiss equipment at our training courses. We have organised symposia that have been supported by Zeiss. We have held expert discussion meetings to review microscopy trends that have involved senior Zeiss staff |
| Collaborator Contribution | Zeiss have made a cash contribution to training courses (received) of £16,250. Zeiss estimate of total value of in-kind staff time for collaboration, training courses and other meetings (including visits of teams from Germany) ?25,000. In addition, Zeiss have also committed over £30,000 in cash and ~£80000 in in kind staff for future training meetings and collaborative visits. Zeiss helped to design the new Systems Microscopy Centre in Manchester and made a 45% discount (value ?350k) for the purchase of equipment in 2011. Zeiss are a formal MICA partner on both Liverpool and Manchester awards from the MRC/BBSRC New Microscopy Initiative. In the award to Manchester they have made a contribution of £614,314 in staff time, development costs and equipment contribution. This involves FCS (developed during this project), luminescence fluorescence imaging, light sheet microscopy and SOFI super-resolution imaging. More recently Zeiss have made a further contribution to our new clinical single cell centre. This includes over £400k in equipment discounts and £25k in cash contribution to training and symposia. Over the years the Zeiss contributions have included them helping us with public understanding of science exhibitions where they loaned equipment, provided support for professional poster preparation and used their delivery services to transport our exhibit materials and equipment to the exhibition venues. This included an exhibition in Buckingham Palace in 2006. Zeiss have sponsored 2-3 meetings per year in Manchester. In 2016 this included a session on light sheet imaging and a session on new confocal imaging technologies. In 2017 they have sponsored an image analysis daya and will sponsor a single cell biology workshop. |
| Impact | Annual training courses MICA collaborative MRC grant MICA collaboration on new single cell centre The relationship with Zeiss has been two way. We have been given the opportunity to be early adopters f new technology and to feedback idease for improvement. We receive very favourable deals on microscope purchases and maintenance contracts. Specific areas of successful collaboration lie in improvements to higher throughput live cell imaging using the confocal microscopes; optimisation of truly dark microscopes for quantitative luminescence imaging and the development of FCS. Multiple workshops organised (2-3 per yeat) |
| Description | Collaboration with University of Tokyo |
| Organisation | University of Tokyo |
| Country | Japan |
| Sector | Academic/University |
| PI Contribution | BBSRC funded partnership to investigate technologies for measurement of protein interactions in single cells |
| Collaborator Contribution | We have visited Toky annually for the past 4 years and there have been similar visits to the UK. There has been exchange of results and reagents. Closer collaboration is planned with exchange of staff in the next year. |
| Impact | Key results have been obtained on measurement of protein interactions. In addition the collaboration has moved into optogenetics and we are exchanging reagents for photoactivation of akt and measurement of effects on the NF-kB pathway. We are planning addition methodology for direct photoactivation of NF-kB signalling.. |
| Start Year | 2009 |
| Description | Hamamatsu Photonics |
| Organisation | PMT Hamamatsu Photonics K.K. |
| Country | Japan |
| Sector | Private |
| PI Contribution | We have advised Hamamatsu on trends in bio-imaging for 20 years. We have provided new data and tested prototype equipment. We have given them an opportunity to display their equipment at our training courses. |
| Collaborator Contribution | They have loaned us equipment and provided privileged discounts for almost 20 years (in kind value well over £100k). They have advised us on new emerging technology. They have assisted by providing staff for our training courses (recent in kind value calculated as £50k. They have provided £10k in cash towards training courses since 2008. A further £4k in cash and £10k has been committed to future training courses. |
| Impact | Annual training courses |