Developing Synchrotron Radiation Circular Dichroism (SRCD) Spectroscopy Methods and Applications for Structural Biology
Lead Research Organisation:
Birkbeck, University of London
Department Name: Biological Sciences
Abstract
Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.
Publications
Meersman F
(2010)
Consistent picture of the reversible thermal unfolding of hen egg-white lysozyme from experiment and molecular dynamics.
in Biophysical journal
Miles AJ
(2013)
Stabilisation of Na,K-ATPase structure by the cardiotonic steroid ouabain.
in Biochemical and biophysical research communications
Miles AJ
(2011)
Correlation of structural and functional thermal stability of the integral membrane protein Na,K-ATPase.
in Biochimica et biophysica acta
Moore B
(2011)
Structural basis of molecular recognition of the Leishmania small hydrophilic endoplasmic reticulum-associated protein (SHERP) at membrane surfaces.
in The Journal of biological chemistry
Platzer G
(2011)
The metastasis-associated extracellular matrix protein osteopontin forms transient structure in ligand interaction sites.
in Biochemistry
Powl AM
(2010)
Synchrotron radiation circular dichroism spectroscopy-defined structure of the C-terminal domain of NaChBac and its role in channel assembly.
in Proceedings of the National Academy of Sciences of the United States of America
Powl AM
(2012)
Transmembrane and extramembrane contributions to membrane protein thermal stability: studies with the NaChBac sodium channel.
in Biochimica et biophysica acta
Sait L
(2020)
Cannabidiol interactions with voltage-gated sodium channels
in eLife
Wallace B
(2011)
Synchrotron radiation circular dichroism (SRCD) spectroscopy: An emerging method in structural biology for examining protein conformations and protein interactions
in Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment
Wallace BA
(2010)
Synchrotron radiation circular dichroism (SRCD) spectroscopy: an enhanced method for examining protein conformations and protein interactions.
in Biochemical Society transactions
| Description | UK access to facilities at a time when there was no such facility in the UK |
| Exploitation Route | no further access is available as the Brookhaven synchrotron has now been shut down |
| Sectors | Education |
| Description | enabled collaborations to develop synchrotron-based methods for spectroscopy |
| First Year Of Impact | 2009 |
| Title | new software tools for CD analyses |
| Description | software and websites and you tube videos about new infrastructure tools developed for CD spectroscopy and structural biology |
| Type Of Material | Improvements to research infrastructure |
| Year Produced | 2018 |
| Provided To Others? | Yes |
| Impact | ongoing development of many new tools for bioinformatics and structural biology resources widespread usage of tools both in the UK and internationally |
| Title | The SP175 reference Data Set |
| Description | Data Set of Reference CD spectra of proteins deposited in the Protein Circular Dichroism Data Bank. protein circular dichoism data bank http://pcddb.cryst.bbk.ac.uk/home.php |
| Type Of Material | Database/Collection of data |
| Year Produced | 2009 |
| Provided To Others? | No |
| Impact | No actual impacts realised to date |
| URL | http://pcddb.cryst.bbk.ac.uk/home.php |
| Description | Collaboration with Brookhaven National Lab (Dr. J.Sutherland) |
| Organisation | Brookhaven National Laboratory |
| Department | National Synchrotron Light Source |
| Country | United States |
| Sector | Public |
| PI Contribution | Enabled us to access the Brookhaven SRCD beamline when there was none in the UK |
| Collaborator Contribution | provided access to instrumentation |
| Impact | students and postdocs had access to SRCD beamline when there was none in the UK. the Brookhaven beamline has now been shut down and our collaborator has retired |
| Start Year | 2009 |
| Description | Running training workshops for students, postdocs and industrial scientists on CD spectroscopy |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Postgraduate students |
| Results and Impact | Teaching Lectures and Practicals in multi-day workshops to provide new skills training and ideas and information for researchers both nationally and internationally |
| Year(s) Of Engagement Activity | Pre-2006,2006,2007,2008,2009,2010,2011,2012,2013,2014,2015,2016,2017,2018 |
| Description | Talks at UK and international sites and meetings: Brazil Synchrotron Symposium, ), University of Vienna Austria (Chemistry Dept), Biochemical Society Training Course (Aston University, UK) |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Talks about Tools and Resources developed in the course of this grant at Universities and International meetings, including the headline talk at the inauguration of the Brazil Synchrotron SRCD beamline (CEDRO) held for the international community of SRCD users and potential users, The Biochemical Society training course on Membrane Proteins at Aston University, the chemistry dept at the University of Vienna. |
| Year(s) Of Engagement Activity | 2022 |
| Description | annual talks for the public |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Public/other audiences |
| Results and Impact | UCL Science Centre Friday Evening Discourses and Birkbeck SET Talks for the Public talks on science for the public (5th, 6th form, and public) annually and lecture/tour at the Wellcome Collection talks no actual impacts realised to date |
| Year(s) Of Engagement Activity | 2006,2007,2008,2009,2010,2011,2012,2013,2014,2015,2016,2017,2018,2019,2020,2021 |
| Description | international advisory boad (germany) |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Other audiences |
| Results and Impact | international advisory board (Germany) |
| Year(s) Of Engagement Activity | 2011,2012,2013,2014,2015,2016,2017,2018 |
| Description | international advisory board (australia) |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Postgraduate students |
| Results and Impact | international advisory board for scientific centre of excellence (australia) |
| Year(s) Of Engagement Activity | 2008,2009,2010,2011,2012,2013,2014,2015,2016,2017 |