Optimising processes for the deconstruction of plant biomass
Lead Research Organisation:
University of Nottingham
Department Name: Sch of Biosciences
Abstract
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Publications
Pullan ST
(2014)
RNA-sequencing reveals the complexities of the transcriptional response to lignocellulosic biofuel substrates in Aspergillus niger.
in Fungal biology and biotechnology
Daly P
(2017)
Expression of Aspergillus niger CAZymes is determined by compositional changes in wheat straw generated by hydrothermal or ionic liquid pretreatments.
in Biotechnology for biofuels
| Description | This grant has served to develop a strong research collaboration between The University of Nottingham and the Joint Bioenergy Research Institute (JBEI) in California, US. As part of this collaboration the response of a filamentous fungus (Aspergillus) to growth on a variety of carbon feed stocks has been examined through analysis of the genes that the fungus expresses. This has shown that the fungal response is substrate specific a finding that may have implications for the use of these feed stocks as sources of bioenergy. |
| Exploitation Route | Since the fungal response to different feed stocks appears to be substrate specific this implies that these results could be used by the bioenergy industry to develop tailor made enzyme cocktails for the breakdown of different substrates and thus improve the efficiency of the bioenergy production process |
| Sectors | Agriculture, Food and Drink,Energy |
| Description | The data from the 138 RNAseq are being used by the JGI in the US to improve the annotation of their genome sequence for Aspergillus. |
| First Year Of Impact | 2014 |
| Impact Types | Policy & public services |
| Description | Partnership with JBEI |
| Organisation | Joint BioEnergy Institute |
| Country | United States |
| Sector | Private |
| PI Contribution | This was a partnership award that funded exchange visits between Nottingham and JBEI. During the initial visit potential collaborations were explored and a joint project identified to examine the response (at a transcriptomic level) of fungi towards a range of pre treated biomass samples. The team at Nottingham then exposed fungi to pre treated wheat straw, Miscanthus and willow. A range of pre treatments including hydrothermal and ionic liquid were utilised in this research. The team extracted RNA samples for the fungi over a time course of 5 to 10 days. There were a total of about 128 sampling points in all. The NA samples were then sent to JBEI for analysis. The annotated genome data were returned to Nottingham and analysis of the data was undertaken to prepare joint publications. |
| Collaborator Contribution | JBEI, in collaboration with the JCI, undertook to carry out RNAseq analysis of all 128 samples from the experiments at Nottingham. The JCI used the sequences obtained to improve their annotation map for the Aspergillus genome. hen JBEI undertook the complete annotation of the RNAseq data and returned this to Nottingham. JBEI then collaborated with the analysis of this data and the preparation of the joint papers. |
| Impact | Two refereed publications have arisen from this collaboration. |
| Start Year | 2010 |