Resources for the Study of the Beneficial Symbioses in Model Legumes
Lead Research Organisation:
John Innes Centre
Department Name: Cell and Develop Biology
Abstract
Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.
Publications
Kryvoruchko IS
(2016)
MtSWEET11, a Nodule-Specific Sucrose Transporter of Medicago truncatula.
in Plant physiology
Pislariu CI
(2012)
A Medicago truncatula tobacco retrotransposon insertion mutant collection with defects in nodule development and symbiotic nitrogen fixation.
in Plant physiology
Verdier J
(2013)
Establishment of the Lotus japonicus Gene Expression Atlas (LjGEA) and its use to explore legume seed maturation.
in The Plant journal : for cell and molecular biology
Wang M
(2013)
LegumeGRN: a gene regulatory network prediction server for functional and comparative studies.
in PloS one
Young ND
(2011)
The Medicago genome provides insight into the evolution of rhizobial symbioses.
in Nature
Zhang JY
(2014)
Global reprogramming of transcription and metabolism in Medicago truncatula during progressive drought and after rewatering.
in Plant, cell & environment
| Description | The collaboration with Noble Foundation lead to the development of a software available on the web to carry out gene network analysis (see link below). The workshops directly lead to a project to sequence a particularly useful ecotype of Medicago truncatula, a model legume used to study plant-microbe symbiotic interactions. This will greatly improve the usefulness of existing genetic resources Medicago research. |
| Exploitation Route | The gene network server can be used to carry out analysis for any species and transcriptomic data type so should be useful to the greater plant biology community. The new genome sequence will be useful to people carrying out comparative genomic studies in legumes, and those interested in legume natural diversity. |
| Sectors | Agriculture, Food and Drink |
| URL | http://legumegrn.noble.org/ |
| Description | The workshops have lead to a project to sequence a particularly useful ecotype of Medicago truncatula, a model legume used to study plant-microbe symbiotic interactions. This will greatly improve the usefulness of existing genetic resources Medicago research. |
| First Year Of Impact | 2012 |
| Title | Medicago truncatula recombinant inbred lines |
| Description | A population of approximately 400 5th generation medicago truncatula lines each with different transposon insertions in different genes |
| Type Of Material | Biological samples |
| Year Produced | 2014 |
| Provided To Others? | Yes |
| Impact | We have successfully used this for the first genetic genetic screen for rhizosphere mutants |
| Description | Databases for Legume Research |
| Organisation | The Samuel Roberts Noble Foundation |
| Country | United States |
| Sector | Charity/Non Profit |
| PI Contribution | We helped develop and test the database and network server |
| Collaborator Contribution | The designed and created and currently host the server and database. |
| Impact | Wang, M., Verdier, J., Benedito, V.A., Tang, Y., Murray, J.D., Ge, Y., Becker, J.D., Carvalho. H., Rogers, C., Udvardi, M., and He, J. (2013) LegumeGRN: a gene regulatory network prediction server for functional and comparative studies. PlosOne DOI: 10.1371/journal.pone.0067434. Verdier J., Torres-Jerez I., Wang M., Andriankaja A., Allen S.N., He J., Tang Y., Murray J.D., and Udvardi M.K. (2013) Establishment of the Lotus japonicus Gene Expression Atlas (LjGEA) and its use to explore legume seed maturation. Plant Journal 74:351-62 |
| Start Year | 2012 |
| Description | Medicago Infection Genetics |
| Organisation | The Samuel Roberts Noble Foundation |
| Country | United States |
| Sector | Charity/Non Profit |
| PI Contribution | We participated in the cloning and characterization of several genes involved in rhizobial infection. |
| Collaborator Contribution | They carried out complementary molecular and physiological studies on the mutants . |
| Impact | Chen D. S., Liu C. W., Roy S., Cousins D., Stacey N., and Murray J. D. (2015) Identification of a core set of rhizobial infection genes using data from single cell-types. Frontiers in Plant Science. 575. doi: 10.1080/15592324.2015.1019982 Horváth B, Domonkos Á, Kereszt A, Szucs A, Ábrahám E, Ayaydin F, Bóka K, Chen Y, Chen R, Murray J. D., Udvardi MK, Kondorosi É, Kaló P. Loss of the nodule-specific cysteine rich peptide, NCR169, abolishes symbiotic nitrogen fixation in the Medicago truncatula dnf7 mutant. Proc Natl Acad Sci U S A. 2015 Sep 23. pii: 201500777. Pislariu C.I.*, Murray J.D* (*co-first authors), Wen, J., Cosson, V., Muni, R.R., Wang, M., Benedito V.A, A, Andriankaja, Cheng, X. , Jerez, I.T., Mondy, S., Zhang, S., Taylor, M.E., Tadege, M., Ratet, P., Mysore, K.S., Chen, R., Udvardi, M.K. (2012) A Medicago truncatula tobacco retrotransposon insertion mutant collection with defects in nodule development and symbiotic nitrogen fixation. Plant Physiology 159:1686-99. Guan D., Stacey N., Liu C., Wen J., Mysore K.S., Torres-Jerez I., Vernie T., Tadege M., Zhou C., Wang Z.Y., Udvardi M.K., Oldroyd G.E., and Murray J.D. (2013) Rhizobial infection is associated with the development of peripheral vasculature in nodules of Medicago truncatula. Plant Physiology 162: 107-115. Lefebvre B, Timmers T, Mbengue M, Moreau S, Hervé C, Tóth K, Bittencourt-Silvestre J, Klaus D, Deslandes L, Godiard L, Murray JD, Udvardi MK, Raffaele S, Mongrand S, Cullimore J, Gamas P, Niebel A, Ott T. (2010) A remorin protein interacts with symbiotic receptors and regulates bacterial infection. Proc Natl Acad Sci U S A 107:2343-8. |
| Start Year | 2011 |
| Title | LegumeGRN |
| Description | It allows users to use their own expression data or public data to model gene regulatory networks. |
| Type Of Technology | Webtool/Application |
| Year Produced | 2013 |
| Impact | Wang, M., Verdier, J., Benedito, V.A., Tang, Y., Murray, J.D., Ge, Y., Becker, J.D., Carvalho. H., Rogers, C., Udvardi, M., and He, J. (2013) LegumeGRN: a gene regulatory network prediction server for functional and comparative studies. PlosOne DOI: 10.1371/journal.pone.0067434. |
| URL | http://legumegrn.noble.org/ |
| Title | LjGEA |
| Description | This is a public gene expression database with analysis functions for the model legume Lotus japonicus. |
| Type Of Technology | Webtool/Application |
| Year Produced | 2013 |
| Impact | Verdier J., Torres-Jerez I., Wang M., Andriankaja A., Allen S.N., He J., Tang Y., Murray J.D., and Udvardi M.K. (2013) Establishment of the Lotus japonicus Gene Expression Atlas (LjGEA) and its use to explore legume seed maturation. Plant Journal 74:351-62 |
| URL | http://bc.noble.org/int/ljgea/ |