Using microscale technologies in tissue engineering of human lung
Lead Research Organisation:
University of Nottingham
Department Name: Sch of Molecular Medical Sciences
Abstract
United States
Publications
Alvarez MM
(2016)
Delivery strategies to control inflammatory response: Modulating M1-M2 polarization in tissue engineering applications.
in Journal of controlled release : official journal of the Controlled Release Society
Bertassoni LE
(2014)
Direct-write bioprinting of cell-laden methacrylated gelatin hydrogels.
in Biofabrication
Ghaemmaghami AM
(2012)
Biomimetic tissues on a chip for drug discovery.
in Drug discovery today
Harrington H
(2014)
Immunocompetent 3D model of human upper airway for disease modeling and in vitro drug evaluation.
in Molecular pharmaceutics
Jabbari, Esmaiel; Kim, Deok-Ho; Lee, Luke
(2014)
Handbook of Biomimetics and Bioinspiration: Biologically-Driven Engineering of Materials, Processes, Devices, and Systems
Rahimi R
(2015)
A Janus-paper PDMS platform for air-liquid interface cell culture applications
in Journal of Micromechanics and Microengineering
Rahimi R
(2016)
A paper-based in vitro model for on-chip investigation of the human respiratory system.
in Lab on a chip
Vishwakarma A
(2016)
Engineering Immunomodulatory Biomaterials To Tune the Inflammatory Response.
in Trends in biotechnology
Vrana NE
(2013)
Engineering functional epithelium for regenerative medicine and in vitro organ models: a review.
in Tissue engineering. Part B, Reviews
Zhao X
(2016)
Photocrosslinkable Gelatin Hydrogel for Epidermal Tissue Engineering.
in Advanced healthcare materials
| Description | During this project we explored the use of 3D hydrogel scaffolds in developing co-cultures of human immune cells and structural cells such as lung epithelium. Key skills developed during this sabbatical include use of photo cross-linking in micropatterning, cell encapsulation and developing 3D hydrogel based scaffolds. Such techniques complemented other strategies we use in developing 3D tissue models including the immunocompetent lung model we are developing as part of a BBSRC funded project. With help from my host research group at Harvard University we have now established the technology for fabrication of microppatterned hydrogels in our laboratory at the University of Nottingham. Such micropatterned scaffolds underpin a number of research projects within my laboratory. Another skill developed during this sabbatical was fabrication of microwells that enable establishing microscale single and co-cultures. Activities during this sabbatical also led to securing major research grant from the European Commission. |
| Exploitation Route | Developing 3D immune competent tissue models for disease modelling |
| Sectors | Pharmaceuticals and Medical Biotechnology |
| Description | This ISIS visit led to establishing a substantive collaboration between research groups at the University of Nottingham and Harvard Medical School. This has already led to a number of ongoing collaborative projects some of which are funded by EU or NIH. We expect this will continue to generate more outputs in areas of immune competent tissue models and immune-bioengineering. |
| First Year Of Impact | 2011 |
| Sector | Pharmaceuticals and Medical Biotechnology |
| Impact Types | Societal |
| Description | Construction of a miniaturized human lymph node model as an alternative to the Local Lymph Node Assay |
| Amount | £120,000 (GBP) |
| Funding ID | NC/K500318/1 |
| Organisation | National Centre for the Replacement, Refinement and Reduction of Animals in Research (NC3Rs) |
| Sector | Public |
| Country | United Kingdom |
| Start | 09/2012 |
| End | 08/2016 |
| Description | Local Immunomodulation around implants by innovative auxiliary hydrogel-based systems encapsulating autologous and phenotype controlled macrophages. |
| Amount | £5,600,000 (GBP) |
| Funding ID | 602694 |
| Organisation | European Commission |
| Sector | Public |
| Country | European Union (EU) |
| Start | 10/2013 |
| End | 09/2017 |
| Description | Personalized And/Or Generalized Integrated Biomaterial Risk Assessment |
| Amount | € 7,992,471 (EUR) |
| Funding ID | 760921 |
| Organisation | European Commission |
| Sector | Public |
| Country | European Union (EU) |
| Start | 12/2017 |
| End | 01/2022 |
| Description | A microfluidic device for studying innate-adaptive immune cell interaction |
| Organisation | Brigham and Women's Hospital |
| Country | United States |
| Sector | Hospitals |
| PI Contribution | Using a microfluidic device to establish a multi cell culture of immune cells under flow conditions |
| Start Year | 2012 |
| Description | Investigating the immunological properties of hydrogels |
| Organisation | Brigham and Women's Hospital |
| Country | United States |
| Sector | Hospitals |
| PI Contribution | A number of collaborative projects have been instigated to analyse the immuno-compatibility of different hydrogels in collaboration with collaborators at Harvard Medical School |
| Start Year | 2012 |