USA - Novel insights into the mechanisms of salt appetite
Lead Research Organisation:
University of Bristol
Department Name: Clinical Science at South Bristol
Abstract
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Publications
Alim FZD
(2019)
Seasonal adaptations of the hypothalamo-neurohypophyseal system of the dromedary camel.
in PloS one
Greenwood M
(2018)
The effects of aging on biosynthetic processes in the rat hypothalamic osmoregulatory neuroendocrine system
in Neurobiology of Aging
Murphy D
(2012)
The hypothalamic-neurohypophyseal system: from genome to physiology.
in Journal of neuroendocrinology
Waki H
(2013)
Excessive leukotriene B4 in nucleus tractus solitarii is prohypertensive in spontaneously hypertensive rats.
in Hypertension (Dallas, Tex. : 1979)
Yoshimura M
(2013)
A c-fos-monomeric red fluorescent protein 1 fusion transgene is differentially expressed in rat forebrain and brainstem after chronic dehydration and rehydration.
in Journal of neuroendocrinology
| Description | Working with our collaborators in Illinois, we have combined transcriptome analysis with proteomics to better understand how the hypothalamus responds to the stress of dehydration, and how this changes with ageing. Dehydration is normally associated with an increase in the secretion of the antidiuretic hormone vasopressin (AVP) from the posterior pituitary, and a concomitant increase in its biosynthesis in the hypothalamus. Elderly people exhibit a diminished capacity to cope with osmotic challenges such as dehydration. We have undertaken a detailed molecular analysis of arginine vasopressin (AVP) biosynthetic processes in the hypothalamus and secretory activity in the posterior pituitary of adult (3 months) and aged (18 months) rats, to provide a comprehensive analysis of age-associated changes to the AVP system. By matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis, we identified differences in pituitary peptides, including AVP, in adult and aged rats under both basal and dehydrated states. In the SON, increased AVPO gene transcription, coincided with reduced AVP gene promoter methylation in aged rats. Based on transcriptome data, we have previously characterized a number of novel dehydration-induced regulatory factors involved in the response of the SON to osmotic cues. We found that some of these increase in expression with age, while dehydration-induced expression of these genes in the SON was attenuated in aged rats. In summary, we show that aging alters the rat AVP system at the genome, transcriptome, and peptidome levels. |
| Exploitation Route | We are continuing to work with our Illinois collaborators on a number of projects related to the hypothalamic control of homeostasis, and the effects of ageing. |
| Sectors | Healthcare,Pharmaceuticals and Medical Biotechnology |
| Description | Saudi Arabia collaboration |
| Organisation | King Saud University |
| Department | Department of Zoology |
| Country | Saudi Arabia |
| Sector | Academic/University |
| PI Contribution | Transcriptomic analysis of Jerboa tissues. |
| Collaborator Contribution | Privision of Jerboa tissues. |
| Impact | Not applicable as yet/ |
| Start Year | 2018 |
| Description | UAE collaboration |
| Organisation | United Arab Emirates University |
| Department | Department of Pharmacology and Therapeutics |
| Country | United Arab Emirates |
| Sector | Hospitals |
| PI Contribution | Transciptomic skills and analysis |
| Collaborator Contribution | Provision og physiological data and samples |
| Impact | No out puts as yet. New collaboration. |
| Start Year | 2015 |