Development of complementary strategies for the analysis of dietary bioactives in foods and biofluids
Lead Research Organisation:
University of East Anglia
Department Name: Norwich Medical School
Abstract
Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.
People |
ORCID iD |
| Colin Kay (Principal Investigator) |
| Description | Outputs to date include preliminary data from pilot studies which suggest that storage of juice concentrates does not result in degradation of anthocyanins but rather the formation of larger molecular weight compounds of unknown structure. We are presently analyzing juices which have been stored under simulated 'house-hold usage' conditions for suspected products of condensation and polymerization. |
| Exploitation Route | The findings from this project will provide evidence for the potential benefits to public health of consuming processed fruit products and in particular beverages; which is important given the current low intake of raw/fresh fruits and vegetables in the diet. The impact of processing and storage on food bioactivity, as explored in this proposal, will identify dietary constituents that may be exploited as novel bioactives with potential functional-food and agricultural implications. Providing proof of the health benefits of anthocyanin-rich juices also holds industrial and agricultural benefit as anthocyanin levels in food crops could be easily modified using pre- and post-harvest manipulation or plant breeding or genetic approaches to produced products of increased bioactivity. As juices are likely to be the major delivery vehicle of anthocyanins to the consumer, this work holds particular relevance to the juice industry and consumers. The project is currently providing valuable training and knowledge transfer between research groups at the University of East Anglia (UEA; UK) and the University of California Davis (UCD; USA). Research staff (Primary Investigators, PhD Students and Research Associates) at the UEA are providing training regarding the extraction and analysis of anthocyanin metabolites in biological samples (urine, serum, faeces) while research staff at UCD are providing training on the extraction and identification of flavonoid polymers in juices, wines and faecal samples. This project will provide valuable training required for the analysis of existing clinical samples and the development of future project grants between the two Universities. This project is of particular importance to phytochemcial researchers as the study designs explored will define experimental approaches for defining the active compounds responsible for the health benefits of flavonoid-rich foods. Ultimately, this project will provide evidence on the structure of novel compounds found in stored juices and there bioactivity. This evidence will be essential to the design of future dietary intervention studies with the goal of determining the effects of juice consumption on chronic disease outcomes. |
| Sectors | Agriculture, Food and Drink,Healthcare,Manufacturing, including Industrial Biotechology,Retail |
| Description | Methodologies discussed and developed as a result of visits between UCD and UEA led to improvement of workflow for establishing the impact of (poly)phenol consumption on the human metabolome, including solid phase extraction and mass spectrometry practices which have been applied to Kay lab analytic practices. |
| First Year Of Impact | 2016 |
| Sector | Agriculture, Food and Drink,Healthcare |
| Description | Norwich Rsearch Park Enterprise and Engagement award, 2014 |
| Amount | £3,000 (GBP) |
| Organisation | Norwich Research Park |
| Sector | Private |
| Country | United Kingdom |
| Start | 10/2014 |
| End | 10/2015 |
| Description | Collaboration with AM, UCD, 2012 |
| Organisation | University of California, Davis |
| Department | Department of Food Chemistry |
| Country | United States |
| Sector | Academic/University |
| PI Contribution | During a visit as part of the present IPA, collaboration with the Department of Food Chemistry was formed to foster future collaboration on the analysis of glutathione conjugates of flavonoids |
| Collaborator Contribution | Future development of potential collaboration |
| Impact | No outputs |
| Start Year | 2012 |
| Description | Collaboration with F.S. of University of California Davis, 2013 |
| Organisation | University of California, Davis |
| Department | Department of Nutrition |
| Country | United States |
| Sector | Academic/University |
| PI Contribution | During a visit as part of the present IPA, collaboration with the Department of Nutrition was formed to foster future collaboration on the analysis of flavonoids in clinical samples from an on-going clinical intervention at Davis. |
| Collaborator Contribution | Potential future clinical collaboration |
| Impact | None as of yet |
| Start Year | 2013 |
| Description | Collaboration with Plant Science Group, Cranfield University, Cranfield, 3013 |
| Organisation | Cranfield University |
| Department | Plant Science Group |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Collaboration established through attendance at BBSRC DRINC dissemination events led to meeting with staff in the department of Health at Cranfield University which lead to a formal collaboration to explore flavonoid polymer formation in juices with the present IPA collaborators at UCD |
| Collaborator Contribution | development of BBSRC project Grant |
| Impact | development of BBSRC project grant |
| Start Year | 2013 |
| Description | Collaborative engagement meeting for programme development with the Dpt of Nutriton at UCD, 2013 |
| Organisation | University of California, Davis |
| Country | United States |
| Sector | Academic/University |
| PI Contribution | Met to help with development of a method at UCD (in the Dpt Nutrition) for analysing anthocyanins in clinical samples and to arrange a PhD student Exchange to send a PhD student (MS) for two weeks to transfer methods to University of California for isolation and identification of anthocyanins, including SPE and MS analysis of biological samples. |
| Collaborator Contribution | Potential for future shared publication |
| Impact | none at this time |
| Start Year | 2013 |
| Description | Methods Transfer Meeting, F.S. 2014 |
| Organisation | University of California, Davis |
| Country | United States |
| Sector | Academic/University |
| PI Contribution | Helping develop a method for anthocyanin metabolite analysis of clinical samples at UCD |
| Collaborator Contribution | Potential for future clinical collaboration |
| Impact | NA |
| Start Year | 2012 |
| Description | PepsiCo Collaboarative Meeting at Cranfield University, Oct 2014 |
| Organisation | PepsiCo |
| Country | United States |
| Sector | Private |
| PI Contribution | Proposal outlined to members of PepsiCo research and nutrition divisions exploring development of processing technology |
| Collaborator Contribution | Met for a 4 hour meeting to explore future engagement and TSB application |
| Impact | None as of yet |
| Start Year | 2014 |
| Description | PhD Research Student Placement (JC) between UCD and UEA, Sept 1-Oct 30 |
| Organisation | University of California, Davis |
| Department | Department of Viticulture and Enology |
| Country | United States |
| Sector | Academic/University |
| PI Contribution | We provided expertise in Q-Trap method development and optimisation and training to the UCD student to take back to UCD and provide support for JCs PhD thesis |
| Collaborator Contribution | Development of methodologies for analysis of cocoa, grape and blackcurrant polymers in solution, transferring previous MS methodologies established at UCD to UEA QTrap. Subsequent work to be progressed in collaborators lab in Germany. |
| Impact | Continuing method development and validation |
| Start Year | 2015 |
| Description | PhD student Exchange (RdH) between UEA and UCD, 2013 |
| Organisation | University of California, Davis |
| Department | Food Science and Technology |
| Country | United States |
| Sector | Academic/University |
| PI Contribution | PhD student Exchange (RdF) between UEA and UCD. PhD student spent two weeks in labs at the University of California transferring methods for isolation and identification of anthocyanins, including SPE and MS analysis of biological samples. |
| Collaborator Contribution | PhD student worked with the department of Viticulture to establish methods for analysis of pyranoanthocyanins in blackcurrant juice to implement in the UK. |
| Impact | methods transfer between research groups |
| Start Year | 2013 |
| Description | Project Development meeting, UCD, 2013 |
| Organisation | University of California, Davis |
| Country | United States |
| Sector | Academic/University |
| PI Contribution | PI travelled to UCD to establish future funding opportunities and to develop a project outline to explore the biological activity of flavonoid polymers. A BBSRC-DRINC project grant outline was progressed and parallel funding streams in the US were explored. |
| Collaborator Contribution | contribution to project development |
| Impact | programme development |
| Start Year | 2013 |
| Description | Project outline meeting, Waterhouse Lab, UCD, 2012 |
| Organisation | University of California, Davis |
| Country | United States |
| Sector | Academic/University |
| PI Contribution | PI travelled to UCD to establish future project goals and identify target juices and wines for polymer method development. Methods from UEA in the analysis of anthocyanin metabolites in human clinical samples were exchanged with PhD students at UCD to implement in their current research projects. Researchers at UCD also provided guidance in the extraction and identification of flavonoid polymers in wines and rat faecal samples. |
| Collaborator Contribution | Expertise shared in flavonoid polymer extraction and analysis |
| Impact | further programme and method development, establishing studentship exchange |
| Start Year | 2012 |
| Description | Project outline meeting, Waterhouse Lab, UCD, 2014 |
| Organisation | University of California, Davis |
| Country | United States |
| Sector | Academic/University |
| PI Contribution | PI travelled to UCD to establish future project goals and plans for student exchange placement |
| Collaborator Contribution | Establish future collaboration and student exchange |
| Impact | established student exchange programme to transfer methods between UEA and UCD |
| Start Year | 2010 |
| Description | Student Tranfer placemetn between UCD and UEA, April 2015 |
| Organisation | University of California, Davis |
| Country | United States |
| Sector | Academic/University |
| PI Contribution | We provided training and expertise in Q-trap method development, optimisation and analysis of small molecules to University of California Davis (UCD) PhD student (J. Cave), and access to extraction and analytical chromatography platforms (not available at UCD) for identifying wine tannins in extracts from aged Californian wines, which were imported as part of the collaboration. |
| Collaborator Contribution | PhD student J. Cave travelled to the University of East Anglia (from University of California Davis; UCD) to transfer methods developed at the UCD for the extraction and analysis of flavonoid polymers from wines, to researchers in the departments of Nutrition to aid in their future work exploring the dynamic equilibrium of bioactives in functional beverages, which is being developed for a BBSRC-DRINC grant application. Researchers at UCD also provided training to UEA PhD student M. Smith for the extraction and identification of flavonoid polymers. |
| Impact | NA |
| Start Year | 2012 |
| Description | Student Tranfer placemetn between UEA and UCD, 2014 |
| Organisation | University of California, Davis |
| Country | United States |
| Sector | Academic/University |
| PI Contribution | PhD student M.S. travelled to UCD for two weeks lab experience |
| Collaborator Contribution | expertise and training |
| Impact | lab methods transfer for anthocyanin polymer extraction and analysis |
| Start Year | 2013 |
| Description | Tour of the MARS research laboratories, Dpt Nutrition, University of California Davis, 2014 |
| Organisation | University of California, Davis |
| Country | United States |
| Sector | Academic/University |
| PI Contribution | expertise and intellectual input shared, including discussion regarding future studentship exchange programme and collaborative research |
| Collaborator Contribution | expertise and intellectual input shared, including discussion regarding future studentship exchange programme and collaborative research |
| Impact | Invited to give an oral presentation to the department and meet with lab manager |
| Start Year | 2014 |
| Description | Invited Lecture to the Norwich Medical School and Norwich Research Park, University of East Anglia, UK, 2014 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Other academic audiences (collaborators, peers etc.) |
| Results and Impact | 20 PhD students, research associates and academics attending a lecture 'Wine quality: insights in wine oxidation chemistry and California wine' presented by collaborator Andrew Waterhouse no direct impact |
| Year(s) Of Engagement Activity | 2014 |
| Description | Invited Seminar to John Innes Centre, 17 April, 2015 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Other academic audiences (collaborators, peers etc.) |
| Results and Impact | Visiting UCD PhD student J. Cave presented 'Wine Pigmented Tannin: Compositional Elucidation by a Complimentary Suite of Mass Spectrometric Techniques' for an Invited Seminar to the John Innes Centre Metabolic Biology Departmental Seminar Series; Norwich Research Park, G34/35 JIC Conference Centre, 17 April, 2015. Talk sparked question, discussion and led to the identification of a number of potential new collaborations. Talk sparked question, discussion and led to the identification of a number of potential new collaborations. |
| Year(s) Of Engagement Activity | 2015 |
| Description | Invited Seminar to MARS research and the department of Nutrition, University of California, 2014 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Other academic audiences (collaborators, peers etc.) |
| Results and Impact | 35 Academics, PhD students and Research associates attended the seminar 'Establishing the effectiveness of dietary flavonoids in improving health, through understanding the structural activity relationships of their metabolites' Presented to the Department of Nutrition & Mars Research Division Sept 25, 2014, UC Davis, CA. no notable impacts |
| Year(s) Of Engagement Activity | 2014 |
| Description | Invited Seminar to the Departments of Food Science & Technology, Nutrition, and Vitaculture University of California, 2013 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Other academic audiences (collaborators, peers etc.) |
| Results and Impact | 35 Academics, Research Associates and PhD students attended an Invited seminar 'Analytical methods for isolation and identification of anthocyanin metabolites' provided by PhD student RdF as part of a student exchange Aid in methods exchange between UCD and UEA |
| Year(s) Of Engagement Activity | 2013 |
| Description | Invited Seminar to the Departments of Food Science & Technology, Nutrition, and Vitaculture, University of California, 2012 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Other academic audiences (collaborators, peers etc.) |
| Results and Impact | 30-50 academics and students from the departments of Viticulture, Food Science, Nutrition and Agriculture, from the University of California Davis, attended the Invited Lecture: Oral presentation - Exploring the Impact of Processing on Anthocyanin Bioactivity and introducing a proposed collaborative project exploring the biological effect of consuming pyranoanthocyanins in juices and wines Oral presentation Established collaborators for future proposals |
| Year(s) Of Engagement Activity | 2012 |
| Description | Invited Seminar to the Departments of Food Science & Nutrition, University of California Davis, 2013 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Postgraduate students |
| Results and Impact | 30+ PhD students and academics attended a Seminar focused on our findings regarding the utility of Anthocyanin metabolites in cardiovascular disease prevention and management and introducing the potential of our collaborative project exploring the biological effect of consuming pyranoanthocyanins in juices and wines. Oral Presentation - 'Exploring the utility of Anthocyanin metabolites in cardiovascular disease prevention and management' promoted the development of further collaboration with the intent of designing future research proposals underway |
| Year(s) Of Engagement Activity | 2013 |
| Description | Invited Seminar to the Departments of Food Science, Nutrition, and Vitaculture, University of California Davis, 2013 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Postgraduate students |
| Results and Impact | 40+ PGR students attended an Invited Seminar to the Departments of Food Science & Technology, Nutrition, and Vitaculture 'exploring The metabolic fate of anthocyanins in humans and further collaborative work describing methods of analysis of anthocyanin metabolites in clinical samples' Oral presentation no actual impacts realised to date |
| Year(s) Of Engagement Activity | 2013 |
| Description | Workshop to the Department of Nutrition, bioanalytical group, University of East Anglia, UK, 2014 |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Postgraduate students |
| Results and Impact | 6 PhD students, 2 Research Associates and 2 PIs attended a workshop 'tannin and pyranoanthocyanidin chemistry: extraction, identification and quantification' presented by UCD partner Andrew Waterhouse. led to development of method |
| Year(s) Of Engagement Activity | 2014 |