Multiple Herbicide Resistance in Grass Weeds: from Genes to AgroEcosystems
Lead Research Organisation:
Newcastle University
Department Name: Sch of Natural & Environmental Sciences
Abstract
In the advanced agricultural production systems of Northern Europe, weed control in cereal crops has become one of the greatest challenges to sustainable intensification, accounting for higher yield losses and greater input costs than all other biological constraints (pests and diseases). The most problematic weeds in cereals in Northern Europe are the wild grasses, notably black-grass (Alopecurus myosuroides), which has become steadily more difficult to control over the last 30 years due to the evolution of herbicide resistance. This resistance assumes two forms: 1) Target site resistance (TSR), whereby the weeds become highly tolerant of herbicides due to mutations in the proteins targeted by these chemicals rendering them less sensitive to inhibition by that herbicide mode of action. 2) Metabolic or multiple herbicide resistance (MHR) where weeds become more tolerant of a broad range of herbicides, irrespective of their chemistry or mode of action, due to a general enhancement in the ability to detoxify crop protection agents. While TSR is now quite well understood and can be countered by the rotational use of herbicides with differing modes of action, the molecular basis and evolutionary drivers which promote MHR are poorly understood and the associated grass weeds very difficult to control using conventional methods. In this 4 year project, we propose to use a combination of molecular biology and biochemistry, ecology and evolution, modeling and integrated pest management to develop better tools to monitor and manage both TSR and MHR in black-grass under field conditions. The project represents a novel agri-systems approach, linking our latest understanding in the molecular biology of herbicide resistance to on farm monitoring and modeling based on a quantitative genetics approach to define the effectiveness of different intervention measures. Through a multidisciplinary consortium, we will integrate knowledge about MHR and TSR at the molecular and biochemical levels and relate this fundamental understanding to resistance phenotypes observed in the field. Selection and breeding experiments will examine the dynamics of selection for resistance, with the intention of determining the genetic architecture of MHR for the first time and its relation to other stresses and life history traits. Data from field monitoring and glasshouse studies will be integrated in ecological, evolutionary and management models with the ultimate aim to design novel management to prevent, delay or mitigate the evolution of herbicide resistance. Finally, the environmental and economic impacts of novel management will be explored. The project therefore has the primary goal of using state of the art approaches spanning molecular biology, weed science, modeling and agronomy to provide new resistance control measures within the life of the programme.
The project is divided into 5 integrated work packages which will address the following questions
1. What are the molecular mechanisms that underpin the evolution of metabolic herbicide resistance?
2. What is the extent of the herbicide resistance problem in UK black-grass populations and what impacts is resistance having on black-grass populations and crop yields?
3. What are the genetic, ecological and agronomic factors that promote and constrain the emergence of herbicide resistance?
4. How can applied evolutionary models be used to manage herbicide resistance?
5. What are the economic and environmental consequences of novel weed and resistance management strategies?
The major outputs will be:
1. A rapid diagnostic toolkit for the on-farm characterisation of herbicide resistance.
2. A resistance audit for the extent and distribution of resistance to the major herbicide modes of action in black-grass.
3. A suite of models to address key questions in the emergence and management of resistance.
4. Management recommendations, together with an analysis of their impacts.
The project is divided into 5 integrated work packages which will address the following questions
1. What are the molecular mechanisms that underpin the evolution of metabolic herbicide resistance?
2. What is the extent of the herbicide resistance problem in UK black-grass populations and what impacts is resistance having on black-grass populations and crop yields?
3. What are the genetic, ecological and agronomic factors that promote and constrain the emergence of herbicide resistance?
4. How can applied evolutionary models be used to manage herbicide resistance?
5. What are the economic and environmental consequences of novel weed and resistance management strategies?
The major outputs will be:
1. A rapid diagnostic toolkit for the on-farm characterisation of herbicide resistance.
2. A resistance audit for the extent and distribution of resistance to the major herbicide modes of action in black-grass.
3. A suite of models to address key questions in the emergence and management of resistance.
4. Management recommendations, together with an analysis of their impacts.
Technical Summary
Our overarching aim is to provide solutions for urgent weed management issues in UK agriculture. Focussed on black-grass (Alopecurus myosuroides), the project will improve our understanding of the evolutionary and agronomic factors underpinning the evolution of multiple herbicide resistance (MHR) induced by enhanced metabolism. We propose to study MHR at all levels, from its molecular genetic basis, through its inheritance, relationship to other weed characteristics, and ultimately its effects on weed numbers, farm management and the wider environment.
We will address 5 major questions
1. What are the molecular mechanisms that underpin the evolution of metabolic herbicide resistance? We will develop molecular techniques that us to characterise MHR in the field and use this as the basis for forming the link between MHR evolution and management.
2. What is the extent of the herbicide resistance problem in UK black-grass populations and what impacts is MHR having on black-grass densities and crop yields? We will use large-scale farm surveys and assess the degree of MHR using molecular tools.
3. What are the genetic and ecological factors that promote and constrain the emergence of herbicide resistance? Quantitative genetic techniques will be used to determine the heritability of MHR and the covariance of this with other life-history characteristics.
4. What are the expected eco-evolutionary dynamics of black-grass populations under different management scenarios? We will develop new models that allow us to explore the possibilities for reducing the rate of evolution of resistance.
5. What are the economic and environmental consequences of novel weed and resistance management strategies? Using tools develop for environmental risk assessment, we will generate predictions for management that account for environmental and economic functions of agricultural systems.
We will address 5 major questions
1. What are the molecular mechanisms that underpin the evolution of metabolic herbicide resistance? We will develop molecular techniques that us to characterise MHR in the field and use this as the basis for forming the link between MHR evolution and management.
2. What is the extent of the herbicide resistance problem in UK black-grass populations and what impacts is MHR having on black-grass densities and crop yields? We will use large-scale farm surveys and assess the degree of MHR using molecular tools.
3. What are the genetic and ecological factors that promote and constrain the emergence of herbicide resistance? Quantitative genetic techniques will be used to determine the heritability of MHR and the covariance of this with other life-history characteristics.
4. What are the expected eco-evolutionary dynamics of black-grass populations under different management scenarios? We will develop new models that allow us to explore the possibilities for reducing the rate of evolution of resistance.
5. What are the economic and environmental consequences of novel weed and resistance management strategies? Using tools develop for environmental risk assessment, we will generate predictions for management that account for environmental and economic functions of agricultural systems.
Planned Impact
This project places a strong emphasis on the translation of its science content to tangible benefits to the sustainability of UK arable agriculture, with the impact of new strategies for weed resistance management being delivered within the life-time of the grant. In a recent comprehensive industry review conducted by the Home Grown Cereals Authority (HGCA), weed control was identified as a key area with 89% of growers and 93% of advisors identifying it was a high or medium priority. The project is therefore directly addressing a major problem in UK agriculture as recognized by the user community. Building on this link to the real benefits of this programme, an understanding of the practical and socioeconomic needs of end users when developing new solutions is central to effective science translation and for these reasons the project will engage from its start with stakeholders. These interactions will steer the development of the applied outputs of the project, such as the development of practical 'in-field' diagnostics, setting the modeling scenarios for resistance management and understanding the wider consequences of new control measures.
Who will benefit from this research ? By better understanding herbicide resistance in grass weeds and how to counteract it, stakeholders who will benefit from this project are the arable farming industry and related professional bodies, policy makers struggling with controlling herbicide resistance in the face of EU directives limiting agrochemical availability and non-government organizations (NGOs) interested in the consequences of sustainable intensification in arable agriculture and associated effects on the environment. An applied output of the work will be to develop new and rapid field diagnostics for different classes of resistance. In addition to the direct effect this will have on UK farmers having better decision making tools in addressing weed control issues, the development of these diagnostics has commercial potential for the development of further products which can be used globally to counteract resistance. The other major impact of the programme will be raising the profile of UK weed research at a time when the respective plant science community is being encouraged to find new practical outlets for its work as well as increasing the awareness of resistance management in agriculture to both funding and policy setting bodies.
How will these benefits be delivered ? Consortium members already have strong links with the prospective stakeholder beneficiaries. Throughout the project, full use will be made the links with the industrial sponsor of the project, the Home Grown Cereals Authority (HGCA), with its broad ranging interactions with the arable farming community. As such, the HGCA will be a primary portal to allow the practical benefits of the project to be rapidly disseminated to end users on a continuous basis through a range of activities including regular 'blogs' . The consortium will also carry out a series of engagement activities with the farming community at agricultural dissemination forums, such as the annual Cereals event and has plans for a conference for stakeholders in the third year to directly disseminate progress on the project. Finally, the effectiveness of these engagement activities will be monitored n an annual basis through regular meetings of the consortium with its stakeholder group to assess the external impact of programme outputs.
Who will benefit from this research ? By better understanding herbicide resistance in grass weeds and how to counteract it, stakeholders who will benefit from this project are the arable farming industry and related professional bodies, policy makers struggling with controlling herbicide resistance in the face of EU directives limiting agrochemical availability and non-government organizations (NGOs) interested in the consequences of sustainable intensification in arable agriculture and associated effects on the environment. An applied output of the work will be to develop new and rapid field diagnostics for different classes of resistance. In addition to the direct effect this will have on UK farmers having better decision making tools in addressing weed control issues, the development of these diagnostics has commercial potential for the development of further products which can be used globally to counteract resistance. The other major impact of the programme will be raising the profile of UK weed research at a time when the respective plant science community is being encouraged to find new practical outlets for its work as well as increasing the awareness of resistance management in agriculture to both funding and policy setting bodies.
How will these benefits be delivered ? Consortium members already have strong links with the prospective stakeholder beneficiaries. Throughout the project, full use will be made the links with the industrial sponsor of the project, the Home Grown Cereals Authority (HGCA), with its broad ranging interactions with the arable farming community. As such, the HGCA will be a primary portal to allow the practical benefits of the project to be rapidly disseminated to end users on a continuous basis through a range of activities including regular 'blogs' . The consortium will also carry out a series of engagement activities with the farming community at agricultural dissemination forums, such as the annual Cereals event and has plans for a conference for stakeholders in the third year to directly disseminate progress on the project. Finally, the effectiveness of these engagement activities will be monitored n an annual basis through regular meetings of the consortium with its stakeholder group to assess the external impact of programme outputs.
Organisations
- Newcastle University (Lead Research Organisation)
- Frontier Agriculture Ltd (Collaboration)
- UNIVERSITY OF NOTTINGHAM (Collaboration)
- Croda Europe Ltd (Collaboration)
- Association of Independent Crop Consultants Ltd (Collaboration)
- Waters Corporation (Collaboration)
- UNIVERSITY OF EXETER (Collaboration)
- Mologic (Collaboration)
- Bayer (Collaboration)
- Syngenta International AG (Collaboration)
- ADAMA Agricultural Solutions Ltd (Collaboration)
- Colorado State University (Collaboration)
- Hutchinson H L Ltd (Collaboration)
- UNIVERSITY OF EDINBURGH (Collaboration)
- Agriculture and Horticulture Development Board (Project Partner)
Publications
Neve P
(2018)
Gene drive systems: do they have a place in agricultural weed management?
in Pest management science
Onkokesung N
(2022)
Molecular diagnostics for real-time determination of herbicide resistance in wild grasses.
in Journal of biotechnology
Patterson EL
(2018)
Glyphosate Resistance and EPSPS Gene Duplication: Convergent Evolution in Multiple Plant Species.
in The Journal of heredity
Ravet K
(2018)
The power and potential of genomics in weed biology and management.
in Pest management science
Renton M
(2014)
Herbicide resistance modelling: past, present and future.
in Pest management science
Sabbadin F
(2017)
Transcriptome sequencing identifies novel persistent viruses in herbicide resistant wild-grasses.
in Scientific reports
Schwarz M
(2021)
Flavonoid-based inhibitors of the Phi-class glutathione transferase from black-grass to combat multiple herbicide resistance.
in Organic & biomolecular chemistry
Torra J
(2021)
Target-Site and Non-target-Site Resistance Mechanisms Confer Multiple and Cross- Resistance to ALS and ACCase Inhibiting Herbicides in Lolium rigidum From Spain.
in Frontiers in plant science
Tétard-Jones C
(2016)
Potential roles for microbial endophytes in herbicide tolerance in plants.
in Pest management science
Tétard-Jones C
(2018)
Changes in the proteome of the problem weed blackgrass correlating with multiple-herbicide resistance.
in The Plant journal : for cell and molecular biology
| Description | The programme has delivered all the projected outputs associated with the individual work-packages (WPs). Importantly, it has also delivered some high level outputs in forming a community of researchers who have delivered the BlackGrass Herbicide Resistance Initiative (BGRI). The BGRI have raised the profile of weed science both nationally and internationally, an output that can only assist the UK's reputation as a centre for agricultural innovation in the future. The BGRI have delivered an impressive set of academic and outreach activities (see annex) and specific outputs of more applied value include. 1)The development of a commercial diagnostic BReD, a pocket device to detect non-target site resistance (NTSR) in black-grass. A world first in herbicide resistance testing, BReD represents a 12 minute simple semi-quantitative test for NTSR in blackgrass (and other grass weeds) and is based on the detection of the protein AmGSTF1, discovered to be a NTSR functional biomarker of NTSR in grasses. The status of AmGSTF1 as intrinsically linked to NTSR in diverse populations of blackgrass collected across the UK has been validated as part of the resistance audit (see 3). BReD will continue to be developed and commercially available to the arable industry following the end of the project through its commercialisation by partner company MoLogic (https://www.mologic-bred.co.uk/) . 2) The generation of intellectual property, through the filing of patent WO 2018/138498A1 by the Newcastle group in 2018 detailing the identification of a new generation of herbicide resistance biomarkers that can serve as the basis of both new diagnostics and targets for chemical inhibition by herbicide synergists. 3) A national audit of resistant blackgrass populations linked to previous management practice. Repeated annual field-surveys have resulted in the creation of a database documenting blackgrass density for ~200 UK fields over 2014-2018, with associated field management data back to 2004. The resistance status of 132 of these surveyed fields was measured through assaying for herbicide resistance, identifying the current widespread extent of resistance to the herbicides 'Atlantis' (mesosulfuron), 'cheetah' (fenoxaprop), and 'laser' (cycloxydim). Statistical analysis linking the management histories with resistance status from WP2.1 has confirmed that there is a strong positive relationship between the historic intensity of herbicide use, and both current blackgrass population density, and herbicide resistance. This has been published in the journal Nature Ecology and Evolution (DOI: https://doi.org/10.1038/s41559-018-0470-1). We have additionally measured the density of blackgrass in over 5000 locations from 2015-2017 allowing us to build up a national scale picture of the distribution of the weed. This work has allowed us to correlate the density of blackgrass with rainfall, land use and soil type. This then allows us to identify areas at risk of future spread of blackgrass. This work has been submitted to the journal Ecology Letters for publication. 4) The research resource of a characterized collection of herbicide resistant blackgrass populations. The BGRI project has generated a unique collection of ca. 200 wild blackgrass populations with extensive data on the phenotypic, genotypic and mechanistic basis of herbicide resistance. These characterised populations have been pivotal to outcomes reported in 3) and 5). Access to these populations has facilitated a CASE PhD studentship with Bayer to explore the diversity of MHR mechanisms, as well as studies to explore population genetics of black-grass, informing the extent of gene flow (dispersal) within- and between-populations. In addition, aunique set of 400 pedigreed blackgrass seed families were created and screened for herbicide resistance and plant life-history / fitness characteristics. Results have been used to quantify the additive genetic-variance underpinning variation in sensitivity to herbicides (including glyphosate), and to calculate the heritability and genetic correlations underpinning ecological traits including flowering times and reproductive fitness. This is a unique blackgrass genetic resource, and is facilitating further experiments into the genetic architecture of NTSR at Rothamsted Research. 5) Underpinning knowledge that can help reduce the likelihood of resistance evolution Statistical approaches have been developed to combine data on molecular mechanisms of resistance, herbicide tolerance status and the history of weed management status. Analyses show that whereas target site resistance (TSR) mutations are significantly associated with intensive use of individual herbicide MOAs, application of herbicide mixes containing multiple MOAs are significantly associated with markers of NTSR. Taken together (with results in 3), these results clearly establish that herbicide diversity alone is not sufficient to mitigate evolution of MHR in blackgrass. Resistance screening has been further extended to the herbicide glyphosate (beyond the original project scope and objectives). Results highlight significant variation in glyphosate sensitivity amongst populations, with some populations showing survivors at field rate application doses (540 g ha-1). Analysis has shown that current glyphosate sensitivity has a heritable genetic basis for selection to act upon, and is significantly correlated with the historic intensity of glyphosate use in these populations. These results suggest that blackgrass populations are evolving resistance to glyphosate and add urgency to current calls for glyphosate stewardship to minimise risks of evolution of resistance. 6) Converting predictive models of herbicide resistance to on farm decisions We developed predictive models of the evolution of TSR and NTSR, and blackgrass density, in response to different control strategies (e.g. herbicide, cultivation, crop rotation). We used these models to find optimal integrated weed management strategies over a 10 year time horizon. This allowed us to convert our understanding of blackgrass population ecology and evolutionary dynamics to practical, economically sustainable, management strategies in the face of evolving resistance. We show that cost effective management requires knowledge of how blackgrass affects crop yields, an important piece of ongoing work we are addressing using our farm management and blackgrass density database. We also show that as resistance evolves optimal weed management strategies should rely more on non-chemical, agronomic control to maximise long-term profitability. 7) Environmental and economic valuation of cost and mitigation of resistance. We developed a new model to allow us to estimate national-scale yield loss and economic cost due to herbicide-resistant blackgrass. The annual winter wheat yield loss across England is 1 million tonnes and the annual cost to the farming community is £0.5bn. This is being submitted to the journal Nature Sustainability. We also developed a new modelling framework to allow concurrent estimation of productivity and economic outcomes as well as environmental end points. This modelling framework is being used in ongoing work assessing trade-offs and synergies using our farm management and blackgrass density database and also for future blackgrass mitigation scenarios. 8) Community of practice. The BGRI has established stakeholder and farmer focus groups and met frequently with those groups to discuss project outcomes in a range of workshop type events. The establishment of a national blackgrass monitoring network has been integral to the success of the BGRI and has depended on close collaboration and participatory research with UK farmers. The BGRI team have attended and presented annually at the Cereals event and at a number of industry, farmer and agronomist events, including the Association of Independent Crop Consultants annual conference (2017 and 2018), and NFU workshop on resistance management and many others (see Annex). Internationally, members of the BGRI consortium were active contributors to the Global Herbicide Resistance Challenge meeting, presenting and helping to organise workshops, raising the profile of the UK as a centre of agricultural research. 9) Predictive tools: the data from the monitoring programme has allowed us to develop a series of models that predict the density of blackgrass as a function of management (e.g. rotation, herbicides, cultivation). Using these models we can better evaluate the effectiveness of alternative management strategies. We have found, for example, that techniques such as spring cropping can be spatially and temporally extremely variable in terms of their effectiveness. This work has been published in Pest Management Science (https://onlinelibrary.wiley.com/doi/abs/10.1002/ps.4759). |
| Exploitation Route | Access to a commercialised herbicide resistance diagnostic and decision system. This part of the project is currently under development and will be co-created with agronomists and farmers. In support of this objective the herbicide resistance diagnostic BReD was commercially launched in June 2018. |
| Sectors | Agriculture, Food and Drink,Chemicals |
| URL | https://www.mologic-bred.co.uk/ |
| Description | The project has used a combination of cutting edge foundational research to underpin new understanding of herbicide resistance and its evolution in the field, that has then allowed us to develop new practical tools for black-grass management and to exchange this knowledge with the arable farming industry on a continuous and participatory basis. The key outcomes from the programme are: 1. Identification of key proteins that are causatively linked to MHR and new understanding of how their functions in resistance could be disrupted in future. 2. Evidence for sub-types of MHR linked to specific herbicide chemistries. 3. The characterisation of latent viruses in blackgrass that could be of value in future biocontrol programmes 4. That epigenetic mechanisms are unlikely to be an evolutionary driver of MHR inheritance 5. The first evidence for active roles for transporter proteins functioning in MHR and their coupled function with detoxifying enzymes. 6. Practical diagnostics for MHR in black-grass that can be used to detect resistance in the field in 10 minutes 7. A national audit of resistant blackgrass populations linked to previous management 8. The research resource of a characterized collection of herbicide resistant blackgrass populations 9. Underpinning knowledge that can help reduce the likelihood of resistance evolution 10. Converting predictive models of herbicide resistance to on farm decisions 11. Environmental and economic valuation of cost and mitigation of resistance. |
| First Year Of Impact | 2018 |
| Sector | Agriculture, Food and Drink,Chemicals |
| Impact Types | Economic |
| Description | Contribution to discussions on glyphosate use and alternatives in the EU (invitation to ENDURE Executive Committee) |
| Geographic Reach | Europe |
| Policy Influence Type | Membership of a guideline committee |
| Description | Member of Weed Resistance Action Group Steering Committee |
| Geographic Reach | National |
| Policy Influence Type | Membership of a guideline committee |
| Impact | The work of the Weed Resistance Action group provides guidelines to support the sustainable use of herbicides in agroecosystems. Whilst hard to quantify the production, distribution and hosting online of guidance documents for the sustainable use of herbicides helps to inform the regulatory environment, provides guidance to growers and other practitioners and impacts on the environmental impacts of herbicide use. |
| URL | https://ahdb.org.uk/knowledge-library/wrag |
| Description | BBSRC CASE studentship funded by industry partner Bayer Crop Science |
| Amount | £22,500 (GBP) |
| Funding ID | BB/P504786/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 10/2017 |
| End | 09/2021 |
| Description | BBSRC DTP (Nottingham) |
| Amount | £123,000 (GBP) |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 09/2017 |
| End | 09/2021 |
| Description | BBSRC Industrial CASE studentship (with Bayer) |
| Amount | £171,965 (GBP) |
| Funding ID | BB/M016420/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 11/2015 |
| End | 10/2019 |
| Description | Critical Roles for Cytochromes P450 in Sustainable Wheat Production |
| Amount | £585,556 (GBP) |
| Funding ID | BB/S005617/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 01/2019 |
| End | 12/2021 |
| Description | Critical Roles for Cytochromes P450 in Sustainable Wheat Production. |
| Amount | £180,000 (GBP) |
| Organisation | Syngenta International AG |
| Sector | Private |
| Country | Switzerland |
| Start | 04/2019 |
| End | 03/2022 |
| Description | GCRF Impact Accelerator Award |
| Amount | £10,000 (GBP) |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 02/2017 |
| End | 03/2017 |
| Description | GCRF Impact Accelerator Award |
| Amount | £46,430 (GBP) |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 01/2017 |
| End | 03/2017 |
| Description | GCRF Impact Accelerator Award (17/18) |
| Amount | £10,000 (GBP) |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 01/2018 |
| End | 01/2018 |
| Description | SWBio DTP |
| Amount | £95,000 (GBP) |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 10/2017 |
| End | 09/2021 |
| Description | Seeding Catalyst Award |
| Amount | £100,000 (GBP) |
| Funding ID | BB/SCA/Newcastle/17 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 11/2017 |
| End | 01/2018 |
| Description | Seeding Catalyst Award |
| Amount | £16,000 (GBP) |
| Funding ID | ISCF-TFP-SA-Newcastle |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 12/2018 |
| End | 02/2019 |
| Description | Seeding Catalyst Award |
| Amount | £9,000 (GBP) |
| Funding ID | ISCF-TFP-SA-Newcastle |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 12/2018 |
| End | 02/2019 |
| Description | Seeding Catalyst Award |
| Amount | £10,000 (GBP) |
| Funding ID | BB/SCA/Newcastle /17 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 11/2017 |
| End | 01/2018 |
| Description | Syngenta Strategic Funding |
| Amount | £350,000 (GBP) |
| Organisation | Syngenta International AG |
| Department | Syngenta Ltd (Bracknell) |
| Sector | Private |
| Country | United Kingdom |
| Start | 07/2014 |
| End | 06/2017 |
| Description | seeding Catalyst Award |
| Amount | £11,000 (GBP) |
| Funding ID | ISCF-TFP-SA-Newcastle |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 12/2018 |
| End | 02/2019 |
| Description | seeding Catalyst Award |
| Amount | £10,000 (GBP) |
| Funding ID | ISCF-TFP-SA-Newcastle |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 12/2018 |
| End | 02/2019 |
| Title | Drought exposure leads to rapid acquisition and inheritance of herbicide resistance in the weed Alopecurus myosuroides |
| Description | This dataset contains data from two-part greenhouse experiments described in the paper: "Vian H. Mohammad, Colin P. Osborne & Robert P. Freckleton (2022) Drought exposure leads to rapid acquisition and inheritance of herbicide resistance in the weed Alopecurus myosuroides". Globally, herbicide resistance in weeds poses a threat to food security. Resistance evolves rapidly through the co-option of a suite of physiological mechanisms that evolved to allow plants to survive environmental stress. Consequently, we hypothesize that stress tolerance and herbicide resistance are functionally linked. We address two questions: (i) does exposure to stress in a parental generation promote the evolution of resistance in the offspring? (ii) Is such evolution mediated through non-genetic mechanisms? We exposed individuals of a grass weed to drought and tested whether this resulted in herbicide resistance in the first generation (F1). In the first experiment ("Exposure to stress in a parental generation promote the evolution of resistance in the offspring"), the effect of three levels of drought ("none, medium and high") is studied in five different nive populations of black-grass. Plant height, above ground biomass and seed weight were measured to estimate the influence of drought treatments on the phenotype. In addition, surviving and dead plants were assessed to evaluate the tolerance of A. myosuroides to drought stress. The drought treatments were initiated 30 days after emergence. In the herbicide assay experiment, the seedlings of F1 of all populations were sprayed with fenoxaprop-P-ethyl herbicide (as "Puma Super" - 69 g a.i. L-1, Bayer Crop Science) using two different doses, a lethal dose (40 g a.i. h-1) and sub-lethal dose (20 g a.i. h-1). 28 days after herbicide application, dead and damaged plants were assessed. Surviving plants were categorised in two ways to account for the differential outcomes of exposure to herbicide: plants were categorised as 'surviving' if they showed no visible effects of herbicide exposure, or 'damaged' if they survived but with obvious effects on above ground tissues. In the second experiment ("The role of non-genetic inheritance"), the effect of two levels of drought ("none and high") is studied in 15 different populations of cloned black-grass plants. Each plant was divided into two clones. Plants were cloned to produce two identical seedlings for the investigation of the role of epigenetic mechanisms in herbicide resistance evolution. After 14 days the cloned plants were re-potted and allowed to establish for one week before initiating a drought stress treatment. One set of the cloned plants was exposed to drought and the second set was grown under well-watered conditions. Plant height, above ground biomass and seed weight were measured, in addition to the number of surviving and dead plants. The herbicide assay was carried out using the five populations that possessed a high rate of viability and germination rate in both treatments ("none" and "high" drought). At the 3-4 leaves stage in September 2018, the seedlings were sprayed with fenoxaprop-p-ethyl herbicide ("Puma Super" - 69 g a.i. L-1, Bayer Crop Science) using two different doses as previously described. 28 days after herbicide application (October 2018) dead and damaged plants were assessed as described above. In terms of both survival and dry mass, we find enhanced resistance to herbicide in the F1 plants when the parents had been exposed to drought. Our results suggest that exposure of weeds to drought can confer herbicide resistance in subsequent generations and that the mechanism conferring heritability of herbicide resistance may be non-genetic. |
| Type Of Material | Database/Collection of data |
| Year Produced | 2021 |
| Provided To Others? | Yes |
| URL | http://datadryad.org/stash/dataset/doi:10.5061/dryad.w3r2280s3 |
| Description | BBSRC USA-UK partnering award: Harnessing next-generation sequencing technologies for eco-evolutionary studies of herbicide resistance and weed biology |
| Organisation | Colorado State University |
| Country | United States |
| Sector | Academic/University |
| PI Contribution | Regular joint lab meetings with Gaines lab. Visits by Neve to CSU and hosting of CSU staff at Rothamsted. A number of joint initiatives, including working together on a GCRF-IAA funded project, co-organisation of a working group and workshop towards establishment of an international consortium for weed genomics. Joint publications. Work towards establishing a collaborative USA-UK black-grass genome project. |
| Collaborator Contribution | As above |
| Impact | Joint papers. Weed genomics workshops. |
| Start Year | 2016 |
| Description | Bayer Crop Science: Neve/Edwards |
| Organisation | Bayer |
| Country | Germany |
| Sector | Private |
| PI Contribution | Neve applied for a BBSRC Industrial CASE studentship. The student is based at Rothamsted Research (Neve as PI), and registered for at Newcastle University (Edwards as co-supervisor). The studentship title is "exploring the evolutionary dynamics of non-target site resistance (NTSR) in Alopecurus myosuroides". The student's work is a direct outcome of previous work completed as part of this grant. We are exploring, the expression of a panel of over-expressed genes that underpin NTSR in a broad set of populations collected during the BGRI project. This work is providing validation for those expression-based markers of NTSR in a broad range of wild, herbicide resistant populations, exploring the co-segregation of markers with various cross-resistance phenotypes in pedigreed black-grass populations and addressing the question 'is the genotypic basis of NTSR similar across a range of populations?' |
| Collaborator Contribution | Bayer are CASE partner, providing scientific and financial support. The student spends a 2-3 month period each year at Bayer's Weed Resistance Competence Centre in Frankfurt, Germany, where she is provided with training and access to equipment, resources and expertise. |
| Impact | No outputs as yet. |
| Start Year | 2015 |
| Description | Beta testing herbicide resistance diagnostics |
| Organisation | Frontier Agriculture Ltd |
| Country | United Kingdom |
| Sector | Private |
| PI Contribution | Field testing of beta type diagnostic for herbicide resistance in black grass |
| Collaborator Contribution | Providing field access and staff to conduct trial |
| Impact | Agronomy and biotechnology. Commercial appraisal- confidential |
| Start Year | 2017 |
| Description | CASE studentship with Croda |
| Organisation | Croda Europe Ltd |
| Country | United Kingdom |
| Sector | Private |
| PI Contribution | Our research and expertise on the control of herbicide resistance using novel chemistries |
| Collaborator Contribution | Expertise in herbicide formulation and advice on creating a spray testing facility at our research farm |
| Impact | Work in progress |
| Start Year | 2015 |
| Description | CASE studentship with Syngenta |
| Organisation | Syngenta International AG |
| Department | Syngenta Crop Protection |
| Country | United Kingdom |
| Sector | Private |
| PI Contribution | Through developing skills in herbicide metabolism we were approached by Syngenta to host this studentship |
| Collaborator Contribution | Radiochemical supplies and expertise in regulatory affairs relating to pesticide registration |
| Impact | Work in progress |
| Start Year | 2015 |
| Description | Collaboration with Adama (global) |
| Organisation | ADAMA Agricultural Solutions Ltd |
| Country | Israel |
| Sector | Private |
| PI Contribution | Development of herbicide resistance diagnostics technology for applications in weeds endemic in the middle east |
| Collaborator Contribution | Field evaluation of testing technology and glass house resistance trials |
| Impact | Incorporates the disciplines of biochemistry, immunology , agronomy and weed science |
| Start Year | 2020 |
| Description | Collaboration with Adama Canada on herbicide resistance diagnostics |
| Organisation | ADAMA Agricultural Solutions Ltd |
| Country | Israel |
| Sector | Private |
| PI Contribution | Development of herbicide resistance diagnostics for wild oat in Canadian wheat belt |
| Collaborator Contribution | Field testing of devices and marketing to farmers |
| Impact | Future commercial development of our UK developed technology for application in Canada |
| Start Year | 2019 |
| Description | Collaboration with Bayer Crop Science |
| Organisation | Bayer |
| Department | Bayer CropScience Ltd |
| Country | United Kingdom |
| Sector | Private |
| PI Contribution | Following on from the meeting in 2014 a follow up visit to workshop participant Dr Roland Beffa, Bayer Crop Science in Frankfurt was arranged. |
| Collaborator Contribution | The ensuing collaboration resulted in the funding of a BBSRC CASE studentship supported by Bayer Crop Science. 'Basis of herbicide resistance and safening in broad-leaf weeds' |
| Impact | Work in progress |
| Start Year | 2017 |
| Description | Collaboration with MoLogic to develop next generation herbicide resistance diagnostic |
| Organisation | Mologic |
| Country | United Kingdom |
| Sector | Private |
| PI Contribution | Identification of new protein biomarkers for resistance testing |
| Collaborator Contribution | Expertise in the Development,ent and and manufacture of lateral flow devices |
| Impact | Development of lateral flow kit. Biotechnology and Biochemistry |
| Start Year | 2020 |
| Description | PhD: Getting to the roots of black-grass control: Crop-weed allelopathic interactions in Alopecurus myosuroides |
| Organisation | Hutchinson H L Ltd |
| Country | United Kingdom |
| Sector | Private |
| PI Contribution | Principal PhD supervisor |
| Collaborator Contribution | Co-supervisors and for Hutchinsons, provision of student training and placement opportunities |
| Impact | Work in progress |
| Start Year | 2017 |
| Description | PhD: Getting to the roots of black-grass control: Crop-weed allelopathic interactions in Alopecurus myosuroides |
| Organisation | University of Nottingham |
| Department | School of Biosciences |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Principal PhD supervisor |
| Collaborator Contribution | Co-supervisors and for Hutchinsons, provision of student training and placement opportunities |
| Impact | Work in progress |
| Start Year | 2017 |
| Description | PhD: Rewinding the tape: experimental evolution of resistance to herbicides in Chlamydomonas reinhardtii |
| Organisation | University of Edinburgh |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Principal PhD supervisor |
| Collaborator Contribution | PhD co-supervisors |
| Impact | Work in progress |
| Start Year | 2017 |
| Description | PhD: Rewinding the tape: experimental evolution of resistance to herbicides in Chlamydomonas reinhardtii |
| Organisation | University of Exeter |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Principal PhD supervisor |
| Collaborator Contribution | PhD co-supervisors |
| Impact | Work in progress |
| Start Year | 2017 |
| Description | Pharmacodynamics of herbicide metabolism in crops and weeds |
| Organisation | Waters Corporation |
| Department | Waters Corporation Centre of Mass Spectrometry Excellence |
| Country | United Kingdom |
| Sector | Private |
| PI Contribution | Expertise in herbicide metabolism studio in crops and weeds |
| Collaborator Contribution | Access to new technologies in ambient and high resolution mass spectrometry. |
| Impact | Involves analytical chemists and biochemists woking together. Outcomes in progress |
| Start Year | 2018 |
| Description | Second level beta testing of resistance diagnostics |
| Organisation | Association of Independent Crop Consultants Ltd |
| Country | United Kingdom |
| Sector | Private |
| PI Contribution | Provision of diagnostic kits and expertise in their use |
| Collaborator Contribution | Testing of black grass field samples |
| Impact | Agronomy and biotechnology. Outputs in progress |
| Start Year | 2018 |
| Title | METHOD AND MEANS RELATING TO MULTIPLE HERBICIDE RESISTANCE IN PLANTS |
| Description | Methods for overcoming multiple herbicide resistance (MHR) in plants using inhibitors of GST suppression of Formula (I), novel chemical inhibitors of Formula (Ia), compositions comprising compounds of Formula (I), and uses and methods relating thereto. |
| IP Reference | WO2009034396 |
| Protection | Patent application published |
| Year Protection Granted | 2009 |
| Licensed | No |
| Impact | The work on the protein has allowed us to develop it as a functional biomarker of herbicide resistance in diagnostics applications. |
| Title | NON-TARGET SITE RESISTANCE |
| Description | The present invention provides novel biomarkers for non-target-site resistance (NTSR) in wild grass, with corresponding methods of identifying NTSR. Corresponding kits and assay devices are also provided. |
| IP Reference | WO2018138498 |
| Protection | Patent application published |
| Year Protection Granted | 2018 |
| Licensed | Commercial In Confidence |
| Impact | Commercialisation of a diagnostic for herbicide resistance in black grass |
| Description | A farmer focus group meeting was held in November 2017 in Cambridgeshire, UK. A total of 26 people attended, including 14 farmers and 5 people from relevant industries. BGRI project members presented their research findings in order to disseminate information to practitioners and other stakeholders. |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Industry/Business |
| Results and Impact | One of the aims of the meeting was to encourage best-practice by sharing the most up-to-date findings. Farmers and other stakeholders alike were very enthusiastic about the project findings presented and they were keen to find out more about how they could implement best practice. The meeting also sought stakeholder input to the project: attendees were presented with some of the project findings and then asked to design management strategies in response. The farmers found the exercise useful as it allowed them to discuss and debate best practice amongst themselves and with industry people and researchers. The strategies created are being used in some of the scenario modelling currently being undertaken. The outcomes will then be fed back to farmers in a subsequent meeting and their input sought to further refine the strategies in light of the new information presented. |
| Year(s) Of Engagement Activity | 2017 |
| Description | AHDB Agronomists conference - December 2018 |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | Paul Neve ran a weed management workshop (day one) and gave a research presentation (day two) |
| Year(s) Of Engagement Activity | 2018 |
| Description | AICC Conference |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Industry/Business |
| Results and Impact | Memebr of BGRI Lola consortium Richard Hull talked on "Herbicide resistance: current status and future threats" at the AICC (Association of Independent Crop Consultants) annual conference, with approximately 150 agronomists in the audience. |
| Year(s) Of Engagement Activity | 2015 |
| Description | American Chemical Society (San Francisco) |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Rob Edwards: Plenary Speaker at the 13th IUPAC International Congress of Pesticide Chemistry, Crop Environment and Public Health Protection Technologies for a Changing World, San Francisco. ACS-AGRO/IUPAC sponsored session: Modes of Action and Resistance Management. |
| Year(s) Of Engagement Activity | 2014 |
| Description | Association of Independent Crop Consultants annual conference |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | Paul Neve presented a talk on the BGRI project. Talk resulted in follow up media interest and an invitation to speak again in 2018. |
| Year(s) Of Engagement Activity | 2017 |
| Description | Association of Independent Crop Consultants annual conference 2018 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | Paul was an invited speaker and gave a talk entitles "Managing glyphosate resistance: risks and responses in global cropping systems and lessons for the UK" |
| Year(s) Of Engagement Activity | 2018 |
| Description | Attendance at European Weed Research Society herbicide resistance working group meeting (David Comont) |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Study participants or study members |
| Results and Impact | The meeting theme was monitoring and surveillance for herbicide resistance in weeds. David Comont gave a talk on epidemiology of herbicide resistance. |
| Year(s) Of Engagement Activity | 2018 |
| URL | http://www.ewrs.org/herbicide_resistance.asp |
| Description | BGRI informal advisory board meeting |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Other audiences |
| Results and Impact | During the occasion of the 2015 pesticide resistance meeting held at Rothamsted Research, the BGRI took the opportunity to convene a small meeting with internationally recognised academics to discuss the project; it's objectives, approaches and anticipated outcomes. The group were impressed by the scope and ambition of the project, which in unrivalled internationally, and made a number of useful suggestions for taking the project forward. |
| Year(s) Of Engagement Activity | 2015 |
| Description | Bayer Crop Science herbicide resistance meeting |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Industry/Business |
| Results and Impact | Bayer Crop Science organised a global meeting to address herbicide resistance problems, responses and solutions. Academics and practitioners were invited from all parts of the world. Neve attended. At this meeting, Neve met with Todd Gaines from Colorado State University, and Neve and Gained resolved to write a successful BBSRC USA-UK partnering award. Neve also had discussions with Chinese scientists engaged in herbicide resistance research, and this has resulted in submission of a joint research proposal via the joint Rothamsted - Chinese Academy of Agricultural Sciences Centre for the Sustainable Intensification of Agriculture (pending). |
| Year(s) Of Engagement Activity | 2015 |
| Description | British Crop Protection Council / Association of Applied Biologists Grass weed workshop |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | Richard Hull chaired this grass weed control workshop session, reviewing and selecting submitted papers. |
| Year(s) Of Engagement Activity | 2018 |
| URL | https://www.nfuonline.com/cpsb_2018_pbf-updated-07_08_2018-2/ |
| Description | British Ecological Society annual meeting |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Other audiences |
| Results and Impact | Presented a poster on the economic impacts of black-grass resistance. |
| Year(s) Of Engagement Activity | 2018 |
| Description | British Ecological Society annual meeting |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Other audiences |
| Results and Impact | Hicks, Freckleton and Neve took part in a special session which explored ecological and evolutionary challenges facing agricultural production. The workshop was convened by Hicks and Freckleton. Neve contributed a talk on herbicide resistance. |
| Year(s) Of Engagement Activity | 2015 |
| Description | Cereals 2015 workshop (Lincoln) |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | Speaker for BBSRC hosted Breakfeast Seminar 'Black-grass resistance research initiative'10th June 2015, Cereals 2015, Lincoln. Presentation of latest Lola consortium results to the farming sector and stakeholder group |
| Year(s) Of Engagement Activity | 2015 |
| Description | Cereals Show 2014 |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | Attendance by a number of members of the BGRI at the annual Cereals industry event. In 2014, the BGRI team presented poster boards outlining the aims and scope of the project. A major outcome from attendance was the recruitment of a number of farmers for the BGRI farm network and farmer focus group. In subsequent years, the team have presented updates to farmers as poster boards and also through a series of annual stakeholder and farmer presentations. |
| Year(s) Of Engagement Activity | 2014,2015,2016,2017 |
| Description | Cereals event: June 13-14, weeds group |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | The weed ecology, evolution and management group presented and discussed latest research findings on blackgrass resistance and management with UK farmers and industry representatives. |
| Year(s) Of Engagement Activity | 2018 |
| Description | Crop Production Magazine / Agrii panel discussion: Black-grass question time |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Professional Practitioners |
| Results and Impact | Paul Neve acted as a panel member for a farmer focused discussion panel. A media article that summarised the discussion and its outcomes was produced. The meeting was attended by ca. 50 farmers and industry representatives |
| Year(s) Of Engagement Activity | 2015 |
| Description | CropTec 2018 |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Industry/Business |
| Results and Impact | Paul Neve gave a talk entitled "farm networks for smart resistance monitoring and management: a blackgrass case study" as well as manning the Smart crop protection exhibits on the Rothamsted stand. |
| Year(s) Of Engagement Activity | 2018 |
| URL | https://www.croptecshow.com/ |
| Description | European Commission: workshop on agronomic, economic and environmental aspects of genetically-modified herbicide tolerant crops |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Policymakers/politicians |
| Results and Impact | Paul Neve gave a presentation on the risks of evolution of weed resistance to glyphosate in systems with heavy reliance on glyphosate-tolerant crops. These insights were considered in respect of ongoing discussions about the introduction of GM crops in the EU. |
| Year(s) Of Engagement Activity | 2014 |
| Description | European Weed Research Society: Herbicide Resistance working group symposium |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Industry/Business |
| Results and Impact | A three-day symposium, bringing together researchers, industry and practitioners to discuss herbicide resistance problems across Europe. Symposium was convened by Neve and attended by Neve and Edwards |
| Year(s) Of Engagement Activity | 2014 |
| Description | Farmer focus workshop |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | A farmer focus workshop was held to disseminate research findings and to obtain farmer input into the next stage of the research. 9 farmers and 3 industry people took part. |
| Year(s) Of Engagement Activity | 2018 |
| Description | Farmer workshop presentation |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Professional Practitioners |
| Results and Impact | Paul Neve gave a 45-minute presentation to a farming group to discuss the evolution and management of resistance to the herbicide, glyphosate. |
| Year(s) Of Engagement Activity | 2018 |
| Description | Global Herbicide Resistance Challenge conference |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Other audiences |
| Results and Impact | Talks and poster were given by a number of the BGRI team (Comont, Onkokesung, Neve, Hull, Crook). Neve organised a workshop on weed genomics for weed management. This workshop and other related follow on activities has led to the establishment of an international weed genomics consortium, including well-advanced plans to generate a reference genome for black-grass. |
| Year(s) Of Engagement Activity | 2017 |
| Description | Interdisciplinary workshop on resistance challenges in agriculture and healthcare |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Other audiences |
| Results and Impact | Paul Neve organised an international workshop in Barcelona, Spain to bring together researchers in herbicide, insecticide, fungicide, antimicrobial, anti-viral, anti-helmintic and anti-cancer resistance. Also attended by Freckleton and Childs |
| Year(s) Of Engagement Activity | 2016 |
| Description | International Weed Genomics Consortium: follow-up meeting |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Industry/Business |
| Results and Impact | Paul Neve co-organised a meeting of the International Weed Genomics Consortium at the Weed Science Society of America annual meeting. At this meeting further steps were taken towards the establishment of a coordinating committee for this global efforts towards generating genomic resources for the world's most important weed species. |
| Year(s) Of Engagement Activity | 2018 |
| Description | International Weed Science Congress |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Other audiences |
| Results and Impact | Paul Neve presented a keynote presentation in the herbicide resistance session at the conference. |
| Year(s) Of Engagement Activity | 2016 |
| Description | Interview on 'Food Unwrapped Episode 7 featuring stories on Wheat, Lard and Apple Juice' Aired Channel 4, 26th February 2018, |
| Form Of Engagement Activity | A press release, press conference or response to a media enquiry/interview |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Media (as a channel to the public) |
| Results and Impact | TV interview with Paul Neve and Rob Edwards on our black grass work |
| Year(s) Of Engagement Activity | 2018 |
| URL | http://www.channel4.com/programmes/food-unwrapped |
| Description | Invited Seminar Speaker Institutional Seminar, 21st October,2019, James Hutton Institute, Dundee. |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Professional Practitioners |
| Results and Impact | Research presentation on agri tech developments at Newcastle |
| Year(s) Of Engagement Activity | 2019 |
| Description | Invited Speaker 58th Annual Meeting of the Weed Science Society of America, 'Herbicide metabolism in crops and weeds: a revisit, current understanding and new insights'. Crystal Gateway Marriott, Arlington, Virginia. 29th Jan-1st Feb 2018. |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Talk on our resistance project at the Weed Science Society of America Annual meeting |
| Year(s) Of Engagement Activity | 2018 |
| Description | Invited annual David Staniforth Lecture, Iowa State University |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Other audiences |
| Results and Impact | Paul Neve was invited to this annual, memorial lecture entitled "Weed science: running to stand still?" |
| Year(s) Of Engagement Activity | 2017 |
| Description | Invited presentation at the Nordic-Baltic Resistance Action Group. |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Industry/Business |
| Results and Impact | Paul Neve was invited to make a presentation "Proactive and Reactive Approaches for Managing Herbicide Resistance in Global Cropping Systems." Neve also took part in a subsequent question and answer session. Participants were particularly interested to hear the UK experience of dealing with a current epidemic of herbicide resistant black-grass, as this species and associated resistance problems are increasing concerns in Nordic and Baltic countries. |
| Year(s) Of Engagement Activity | 2018 |
| Description | Invited speaker Cereals 2019, Innovation and Technology Theatre, 'Biologicals for Disease and Pest Control', 12th June 2019, Lincoln. |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | Presentation and demonstration to farmers at Cereals 2019 event. |
| Year(s) Of Engagement Activity | 2019 |
| Description | Invited speaker Global Herbicide Resistance Challenge 2017, Denver, Colorado May 14-18, 2017. |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Talk by group at international resistance conference following invitation |
| Year(s) Of Engagement Activity | 2017 |
| Description | Invited speaker and demonstrator at BBSRC Innovation Hub, Oxford Farming Conference, Oxford 3rd January 2018. |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Industry/Business |
| Results and Impact | Invited demonstration of black grass resistance diagnostics at BBSRC innovation hub |
| Year(s) Of Engagement Activity | 2018 |
| Description | Invited speaker and panellist at Crop Protection Seminar, CropTec, East of England Showground, Peterborough, 29th November 2016. |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Industry/Business |
| Results and Impact | Invited presentation at practical seminar series on latest innovations in crop protection |
| Year(s) Of Engagement Activity | 2016 |
| Description | Invited speaker at Velcourt Annual Conference, 'Black-grass herbicide resistance mechanisms and diagnostics' Oxford Belfry, Thame, Oxon 11th January |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Industry/Business |
| Results and Impact | Presented work on agridiagnostics to national Velcourt conference |
| Year(s) Of Engagement Activity | 2018 |
| Description | Invited speaker, Global food Security Institute, University of Saskatoon, Canada, 22nd March 2017 |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | UK-Canadian researcher meeting on agritech opportunities for joint working sponsored by IUK and FCO |
| Year(s) Of Engagement Activity | 2017 |
| Description | Joint Organiser of the Rank Prize Fund Mini-Symposium 'New Crop Protection', Wordsworth Hotel, Grasmere, Cumbria 13th-16th May 2019. |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Hosted a workshop supported by the RANK prize committee on modern approaches to crop protection attended by early career researchers. |
| Year(s) Of Engagement Activity | 2019 |
| Description | KTN Conference (Birmingham) |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Industry/Business |
| Results and Impact | Plenary speaker at The Knowledge Transfer Network 'Sensors in Agriculture' meeting, Birmingham, 23rd February 2016. Disseminating practical outcomes of BBSRC Lola grant in terms of development of diagnostics |
| Year(s) Of Engagement Activity | 2016 |
| Description | Latin American Weed Science Congress |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Other audiences |
| Results and Impact | Paul Neve was invited to give a plenary, invited lecture at this conference in Buenos Aires, Argentina. He reported on the 'gene to landscape' approaches for studying herbicide resistance that have been developed by the BGRI project. Neve made a number of contacts and has continued discussions about potential future collaborative projects in Latin America. One GCRF-IAA project was funded as a result of links made at this event. |
| Year(s) Of Engagement Activity | 2015 |
| Description | NFU pesticide resistance workshop |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Industry/Business |
| Results and Impact | The National Farmers Union convened a meeting at their Stoneleigh HQ to discuss the current national situation relating to insecticide and herbicide resistance. Experts shared perspectives on the current extent of resistance in the UK and on current best management recommendations. There was a particular focus on establishing mechanisms for effective and consistent communication, particularly in relation to current threats of glyphosate resistance. Paul Neve presented on risks of glyphosate resistance. Stephen Foster (Rothamsted) on current knowledge and management recommendations relating to resistance to pyrethroid insecticides. |
| Year(s) Of Engagement Activity | 2017 |
| Description | Northern Farming Conference |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Industry/Business |
| Results and Impact | Plenary Speaker Northern Farming Conference, Hexham 11th November 2015, 'Crop Protection; from a reactive to a proactive approach' Influencing farming industry into changing pesticide resistance management practice |
| Year(s) Of Engagement Activity | 2015 |
| Description | Oxford Farming Conference |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | BGRI Lola consortium member Paul Neve gave an overview of the BGRI project at a BBSRC event during the Oxford Farming Conference. |
| Year(s) Of Engagement Activity | 2015 |
| Description | Plenary Speaker, 'New Applications for Mass Spectrometry in Crop Protection', American Society of Mass Spectrometry, Indianapolis, 3rd June 2017. |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Industry/Business |
| Results and Impact | Presentation on recent advances in using MS to characterise herbicide resistance types |
| Year(s) Of Engagement Activity | 2017 |
| Description | Plenary speaker 'Advances in IPM 2017. Re-inventing agriculture to reduce dependency on pesticides.' Association of Applied Biologists, Harper Adams University, Newport. 25-26 September 2017. |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Industry/Business |
| Results and Impact | Talk to industry and applied practitioners working in crop protection on advances in weed resistance management |
| Year(s) Of Engagement Activity | 2017 |
| Description | Plenary speaker British Crop Protection Congress 2019 |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Industry/Business |
| Results and Impact | Plenary speaker British Crop Protection Congress 2019 'Using science to deliver both quality food and enhanced environmental protection', Brighton Metropole Hotel, 19-20th November 2019. |
| Year(s) Of Engagement Activity | 2019 |
| Description | Plenary speaker British Crop Protection Congress 2019 'Using science to deliver both quality food and enhanced environmental protection', Brighton Metropole Hotel, 19-20th November 2019. |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Industry/Business |
| Results and Impact | Plenary speaker British Crop Protection Congress 2019 'Using science to deliver both quality food and enhanced environmental protection', Brighton Metropole Hotel, 19-20th November 2019. Influencing industry and regulators over the use of diagnostics in crop protection. |
| Year(s) Of Engagement Activity | 2019 |
| Description | Plenary speaker, Farms.Com Precision Agriculture Conference and Agritech Showcase |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Industry/Business |
| Results and Impact | Plenary speaker, Farms.Com Precision Agriculture Conference and Agritech Showcase, RBC Place, London Ontario 29th-30th January 2020. |
| Year(s) Of Engagement Activity | 2020 |
| Description | Plenary speaker, Farms.Com Precision Agriculture Conference and Agritech Showcase, Conference Centre, London Ontario 29th-30th January 2020 |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Plenary speaker, Farms.Com Precision Agriculture Conference and Agritech Showcase, Conference Centre, London Ontario 29th-30th January 2020 |
| Year(s) Of Engagement Activity | 2020 |
| Description | Plenary speaker, 1st International Molecular Plant Protection Congress, Adana, Turkey 10-13th April 2019. |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Plenary speaker, 1st International Molecular Plant Protection Congress, Adana, Turkey 10-13th April 2019. Update on outreach and commercialisation activities to a crop protection international audience |
| Year(s) Of Engagement Activity | 2019 |
| Description | Plenary speaker, 56th Annual BCPC Weeds Review 'Emerging Science for Weed Control', NIAB, Cambridge 14th November 2019. |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Industry/Business |
| Results and Impact | Plenary speaker, 56th Annual BCPC Weeds Review 'Emerging Science for Weed Control', NIAB, Cambridge 14th November 2019. Gave an update on the outputs of the LOLA grant |
| Year(s) Of Engagement Activity | 2019 |
| Description | Presentation to farmers at Indigro Academy day (David Comont) |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Professional Practitioners |
| Results and Impact | David Comont presented an update to 30 farmers on Rothamsted's research on the current UK status of herbicide resistant blackgrass. |
| Year(s) Of Engagement Activity | 2018 |
| Description | Public Lecture (Newcastle) |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Public/other audiences |
| Results and Impact | Cameron -Gifford Public Lecture, 'Crop Protection in 21st Century Agriculture', Newcastle University, 3rd March. |
| Year(s) Of Engagement Activity | 2015 |
| Description | Seminar and visit: Monsanto, St Louis, USA. |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Industry/Business |
| Results and Impact | Paul Neve was invited to attend a two-day 'meet and greet' with Monsanto scientists working on herbicide and insecticide resistance evolution. Extensive discussions considered the development and application of modelling tools for predicting optimal management for genetically-modified herbicide resistant crops. In particular, Monsanto scientists were keen to engage in discussions to determine how they might support future funded research at Rothamsted to develop models to explore best resistance management for the deployment of crop varieties with multiple herbicide resistance traits. As part of the visit, Paul Neve gave a research seminar on modelling and epidemiology of herbicide resistance. |
| Year(s) Of Engagement Activity | 2018 |
| Description | Session Chair Clyde Higgs Scholars' Workshop |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Postgraduate students |
| Results and Impact | Session Chair Clyde Higgs Scholars' Workshop, Chesford Grange, Kenilworth 29-30th November 2019. |
| Year(s) Of Engagement Activity | 2019 |
| Description | Speaker at Herbicide Resistance Diagnostics Demonstration |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Industry/Business |
| Results and Impact | Speaker at Herbicide Resistance Diagnostics Demonstration at BASIS and FACTS Update Day, University of Lincoln, 6th February 2020 |
| Year(s) Of Engagement Activity | 2020 |
| Description | Stakeholder Workshop |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Industry/Business |
| Results and Impact | The BGRI hosted our inaugural Farmer Focus Group with a small group of participating farmers who will contribute to the project throughout the 5 years. Rob Freckleton, Helen Hicks and Paul Neve gave project updates and led discussions around approaches to black-grass management and future directions for research. February 2015 |
| Year(s) Of Engagement Activity | 2015 |
| Description | Stakeholder group meeting |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Industry/Business |
| Results and Impact | The Lola BGRI held its first stakeholder meeting at ZSL in London. It was attended by representatives from 14 organisations. The day included research updates from each part of the project and discussion groups in the afternoon. See our news article, First BGRI stakeholder meeting, on the news page for more details. |
| Year(s) Of Engagement Activity | 2014 |
| Description | Talk at industry event |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Industry/Business |
| Results and Impact | Invited workshop speaker at 'Crop Protection' session, The CropTec show November 28-29th 2018, East of England Showground, Peterborough. |
| Year(s) Of Engagement Activity | 2018 |
| Description | Talk at industry forum |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Industry/Business |
| Results and Impact | Invited plenary speaker 'AgChem and Technology Europe,' Novotel West ,20th- 21st November, Novotel West, London. |
| Year(s) Of Engagement Activity | 2018 |
| Description | Workshop (Great Yorkshire Show) |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Industry/Business |
| Results and Impact | Host and speaker for Great Yorkshire Show Breakfeast Seminar 'Black-grass resistance research initiative' 16th July 2015, Yorkshire Agricultural Society, Harrogate Latest outcomes for Lola grant |
| Year(s) Of Engagement Activity | 2015 |
| Description | Zoo Nights - public-facing evening event |
| Form Of Engagement Activity | Participation in an open day or visit at my research institution |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Public/other audiences |
| Results and Impact | I ran a science stand at an evening event at London Zoo. I engaged with visitors, telling them about the project and, more generally, about the impact of agriculture on the environment. |
| Year(s) Of Engagement Activity | 2018 |