High resolution cryo-electron microscopy of clathrin cage complexes
Lead Research Organisation:
University of Warwick
Department Name: School of Life Sciences
Abstract
USA
People |
ORCID iD |
| Corinne Smith (Principal Investigator) |
Publications
Smith SM
(2017)
Weak Molecular Interactions in Clathrin-Mediated Endocytosis.
in Frontiers in molecular biosciences
Morris KL
(2019)
Cryo-EM of multiple cage architectures reveals a universal mode of clathrin self-assembly.
in Nature structural & molecular biology
Dannhauser PN
(2017)
CHC22 and CHC17 clathrins have distinct biochemical properties and display differential regulation and function.
in The Journal of biological chemistry
| Description | As a result of this collaborative visit we have developed new methods to discover the structure of cage complexes using 3D electron microscopy |
| Exploitation Route | We are using these methods currently to obtain high resolution structures of cage complexes. Once we have completed development these methods may be of use to others working with related samples. |
| Sectors | Pharmaceuticals and Medical Biotechnology |
| Description | Alert14 |
| Amount | £1,500,000 (GBP) |
| Funding ID | BB/M01228X/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 01/2015 |
| End | 12/2015 |
| Description | Clathrin assembly regulation of glucose metabolism |
| Amount | £491,534 (GBP) |
| Funding ID | BB/V001434/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 12/2020 |
| End | 11/2023 |
| Description | Project grant - Exploiting direct electron detection to resolve protein-protein interactions in clathrin-mediated endocytosis |
| Amount | £550,000 (GBP) |
| Funding ID | BB/N008391/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 04/2016 |
| End | 03/2019 |
| Title | Sorting of cage types for single particle analysis |
| Description | Using artificial cage types we have developed a methodology for sorting heterogeneous cage types within our clathrin sample to obtain 3D structures. |
| Type Of Material | Improvements to research infrastructure |
| Year Produced | 2019 |
| Provided To Others? | Yes |
| Impact | Shortly after publication in Nature Structural and Molecular Biology (Morris et al 2019) a preprint came out in BiRXiv using this method and referencing this work. |
| URL | https://github.com/kylelmorris/Morris_2019_NSMB |
| Description | Improving image analysis of clathrin cage complexes |
| Organisation | University of California, San Francisco |
| Country | United States |
| Sector | Academic/University |
| PI Contribution | Biochemical knowledge of the system and sample preparation |
| Collaborator Contribution | Use of cryo-electron microscope and direct electron detector. Advice on image processing approaches. |
| Impact | As a result of the collaboration we were able to obtain preliminary data for a full project grant application to BBSRC which was successful. We have also now succeeded in publishing our work in Nature Structural and Molecular Biology (Morris et al, 2019) |
| Start Year | 2013 |
| Description | Inaugural lecture |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Other audiences |
| Results and Impact | Delivered professorial inaugural lecture to at least 50-100 people in person and online that presented my research and career path to a broad audience. The lecture stimulated questions and positive feedback and will be reported in the departmental newsletter. |
| Year(s) Of Engagement Activity | 2022 |
| Description | Royal Society of Biology Blog |
| Form Of Engagement Activity | Engagement focused website, blog or social media channel |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Public/other audiences |
| Results and Impact | I wrote a short blog for the Royal Society of Biology explaining my research to a general audience. Informal feedback from colleagues and via social media was positive. |
| Year(s) Of Engagement Activity | 2016 |
| URL | https://blog.rsb.org.uk/clathrin-maintaining-cell-health-in-geometric-style/ |