Visit to the laboratory of Professor Alison Barth to learn in vivo optogenetics, multicellular recording and re-establish the collaboration
Lead Research Organisation:
Keele University
Department Name: Inst for Science and Tech in Medicine
Abstract
United States
Publications
Butcher JB
(2022)
A requirement for astrocyte IP3R2 signaling for whisker experience-dependent depression and homeostatic upregulation in the mouse barrel cortex.
in Frontiers in cellular neuroscience
| Description | Re-established collaboration. New experiments planned in the summer 2017, which have been moved to January 2018 and executed. Plans have been delineated for the summer visit (2018) Set-up optogenetic in vivo at Keele and in vitro at Aston and executed all experiments that required technology learned at Carnegie Mellon, including controls. All of them have been listed in the parallel grant portfolio (in vivo and in vitro optogenetics). Calcium recording in vivo learned in Barth lab is necessary to met the objectives of the next BBSRC grant that we have just applied for, but were turned down. Also executed a few experiments with use of silicon probes gaining preliminary data for the grant application. |
| Exploitation Route | We are using technology that we learned at Carnegie Mellon in our present and we will use in our future approaches, that is optogenetics, calcium imaging in vivo and silicon probes recording. We see the interest in this technology from the surrounding labs and we are willing to help in the matter. WE also started collaboration with a group from the Nencki Institute , Warsaw, requiring optogenetics. This new collaboration enable us also to learn functional optical recoding. |
| Sectors | Education,Healthcare,Pharmaceuticals and Medical Biotechnology |
| Description | increasing our general understanding of brain function |
| First Year Of Impact | 2015 |
| Sector | Education |
| Impact Types | Societal |
| Description | Travel Grant |
| Amount | £698 (GBP) |
| Organisation | Physiological Society |
| Sector | Charity/Non Profit |
| Country | Global |
| Start | 08/2018 |
| End | 09/2018 |
| Title | new method of stimulating astrocytes via IP3R using opto - and chemogenetics |
| Description | optogenetic via Gq coupled melanopsin (OPN-GFAP-CNP) and a chemo-genetic via "Designer Receptor Exclusively Activated by Designer Drug" with GFAP promoter (GFAP-Gq-DREADD) can be used to target astrocyte Gq-IP3 signalling |
| Type Of Material | Technology assay or reagent |
| Provided To Others? | No |
| Impact | using these methods one can drive astrocytes via their major known pathways |
| Description | Cortical representation of temperature in rodents |
| Organisation | University of Notre Dame |
| Department | Department of Biological Sciences |
| Country | United States |
| Sector | Academic/University |
| PI Contribution | Hands on expertise in extracellular recording in vivo, including the surgical skills and the ideas, part of subsistence and travel support. |
| Collaborator Contribution | Ideas, animals, consumables, part of subsistence and travel support |
| Impact | This is the collaboration in frame of neuroscience, but still probably multidisciplinary. This is on-going research. We have done several recordings for the mice insular and somatosensory cortices and also from the superior colliculus. Only in the latter location we managed to find neurones responding to cold temperature. WE are at present moving with this project to Arch optogenetics and also multicellular recording from insula. |
| Start Year | 2012 |
| Description | Development of a classifier to differentiate between classes of neurones during in vivo recording |
| Organisation | Carnegie Mellon University |
| Department | Department of Biological Sciences |
| Country | United States |
| Sector | Academic/University |
| PI Contribution | The collaboration re-started at the beginning of this year with solving technical issues associated with silicon probe recordings in vivo. This collaboration can take many different routes. |
| Collaborator Contribution | participation in the cost of the visit to Pittsburgh and offering brilliantly equipped lab and technical help. |
| Impact | Solving technical issues associated with silicon probe recording |
| Start Year | 2018 |
| Description | Effects of learning involving vibrissae on responses across the layers of the barrel cortex |
| Organisation | Carnegie Mellon University |
| Department | Department of Biological Sciences |
| Country | United States |
| Sector | Academic/University |
| PI Contribution | Taking part in setting up and executing the experiments |
| Collaborator Contribution | Provided animals, consumables and training and covered the cost of transportation and subsistence plus the leading role during setting up and the execution of all parts of the experiments |
| Impact | This is interdisciplinary collaboration. Some results are already collected and they are processed at present |
| Start Year | 2018 |
| Description | Mechanisms of plasticity in the mouse barrel cortex |
| Organisation | Cardiff University |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Electrophysiological recordings from wt and genetically modified mice in vivo, travel money, consumables, lab work |
| Collaborator Contribution | genetically modified and wt mice, some recordings, travel and accommodation money |
| Impact | Journal Papers Fox K. Wright N., Wallace H. and Glazewski S. (2003) The origin of cortical surround receptive fields studied in the barrel cortex. J. Neurosci. 23: 8380-8391. *Hardingham N.R., *Glazewski S., *Pakhotin P., Giese K.P., Chapman P. and Fox K. (2003) The effect of a CaMKII T286A point mutation on experience-dependent plasticity and LTP in mouse barrel cortex. J. Neurosci., 23: 4428-4436.*shared first authorship. Wright N., Glazewski S. Hardingham N., Philips K., Pervolaraki E. and Fox K. (2008) Laminar analysis of the role of GluR1 in experience-dependent and synaptic depression in barrel cortex. Nature Neurosci. 11: 1140-1142. Dachtler J., Hardingham N., Glazewski S., Wright N., Blain E., Fox K. (2011) Experience-dependent plasticity acts via GluR1 and a novel NOS1-dependent synaptic mechanism in adult cortex. J. Neurosci. 31: 11220-11230. Meeting papers: Glazewski S., Sprengel R. and Fox K. The effect of deleting AMPA receptor subunit GluR A on experience-dependent plasticity in mouse barrel cortex. Society for Neuroscience Annual Meeting, Orlando 2002. Fox K, Wright N., Wallace H and Glazewski S. The origin of cortical surround receptive fields studied in the barrel field. Barrel Meeting, Lausanne, 2004. Dachtler, J.; Wright, N. F.; Glazewski, S.; Fox, K. D. The role of neuronal nitric oxide in experience-dependent plasticity in the mouse barrel cortex. Society for Neuroscience Annual Meeting, Washington D.C, 2008. Dachtler J., Hardingham N. R., Glazewski S.; Fox K. D. Gender differences in neuronal nitric oxide synthase-dependent neocortical plasticity. Society for Neuroscience Annual Meeting, Chicago, 2009. Dachtler, J.; Wright, N. F.; Glazewski, S.; Fox, K. D. The role of neuronal nitric oxide in experience-dependent plasticity in the mouse barrel cortex. Society for Neuroscience Annual Meeting, Washington D.C, 2008. Glazewski, S., Greenhill, S., Fox, K. Experience-dependent Homeostatic plasticity in the barrel cortex. Society for Neuroscience Annual Meeting, San Diego, 2013. Glazewski, S., Greenhill, S., Butcher, J., Fox, K. Experience-dependent Homeostatic plasticity in the barrel cortex. Forum of European Neuroscience, Milan, Italy, 2014. |
| Start Year | 2006 |
| Description | Brain Awareness Week in Krakow and Lublin, Poland |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Public/other audiences |
| Results and Impact | Brain Awareness Week in Krakow and Lublin. Individual lectures in frame of this event in Krakow with media attendance and broad spectrum of the public (well over 500 participants in each case). The purpose was to familiarise the public with recent achievements in chosen areas of neuroscience, including the newest technology, prospects, impact on society and medicine. This was followed by the discussion and media interview. |
| Year(s) Of Engagement Activity | 2007,2009,2015 |
| Description | School visits (two schools..one comprehensive and one grammar) |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Schools |
| Results and Impact | Different forms of activities. Mostly lecturing followed by the discussion, questions and answers. Sometimes demonstrations. The aim is to popularise neuroscience. not measurable |
| Year(s) Of Engagement Activity | 2012,2013,2014,2015,2016 |
| Description | Speaker in frame of 13th Electrophysiological Conference, Warsaw , Poland |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Formal talk to professionals including the data collected during both grants |
| Year(s) Of Engagement Activity | 2018 |
| Description | Talk in Carnegie Mellon University |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Undergraduate students |
| Results and Impact | The talk about homeostatic plasticity with the discussion |
| Year(s) Of Engagement Activity | 2018 |
| Description | Teaching Mobility Lublin |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Undergraduate students |
| Results and Impact | 8h of lecturing (neuroscience, including the role of astrocytes in various processes in the CNS)...50-70 people attending each of them |
| Year(s) Of Engagement Activity | 2016 |
| Description | Teaching Mobility Lublin |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Undergraduate students |
| Results and Impact | 8 hours of lecturing delivered in frame of Erasmus Teaching Mobility with discussion afterwards plus exchange on research and teaching |
| Year(s) Of Engagement Activity | 2010,2011,2012,2013,2014,2015,2016,2017,2018 |
| Description | University visit in frame of Teaching Mobility (Krakow and Lublin, Poland) |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | Yes |
| Geographic Reach | International |
| Primary Audience | Undergraduate students |
| Results and Impact | Series of lectures concerning different aspects of neuroscience (each year the different set) and in many cases linked to the understanding every day human activities. The lectures also includes large technological components, including their possible impact ion every day life. There is always long discussion at the end of each lectures followed by private talks. The main purpose is to popularise neuroscience , which is a rarity in Poland not measurable |
| Year(s) Of Engagement Activity | 2008,2009,2010,2011,2012,2013,2014,2015,2016 |