"Behavioural assays for structural plasticity and repair in the central nervous system of Drosophila"
Lead Research Organisation:
University of Birmingham
Department Name: Sch of Biosciences
Abstract
Germany
People |
ORCID iD |
| Alicia Hidalgo (Principal Investigator) |
Publications
Li G
(2020)
A Toll-receptor map underlies structural brain plasticity.
in eLife
Li G
(2020)
Adult Neurogenesis in the Drosophila Brain: The Evidence and the Void.
in International journal of molecular sciences
| Description | Learned methods new to my team. Most particularly, this involved techniques to investigate behaviour in Drosophila: the Buridan arena and optomotor response for the visual system, and learning and memory. The results obtained with this grant form part of a larger project. This work is part of a larger body of work, which has just recently been published in eLife: Guiyi Li, Manuel G Forero, Jill S Wentzell, Ilgim Durmus, Reinhard Wolf, Niki C Anthoney, Mieczyslaw Parker, Ruiying Jiang, Jacob Hasenauer, Nicholas James Strausfeld, Martin Heisenberg, Alicia Hidalgo (2020) "A Toll receptor map underlies structural brain plasticity" eLife DOI: 10.7554/eLife.52743 |
| Exploitation Route | it has brought new techniques to my lab. Published in Li et al 2020 eLife DOI: 10.7554/eLife.52743, see link above |
| Sectors | Healthcare,Pharmaceuticals and Medical Biotechnology |
| URL | https://elifesciences.org/articles/52743 |
| Description | The findings from this grant were used in a recent publication: Guiyi Li, Manuel G Forero, Jill S Wentzell, Ilgim Durmus, Reinhard Wolf, Niki C Anthoney, Mieczyslaw Parker, Ruiying Jiang, Jacob Hasenauer, Nicholas James Strausfeld, Martin Heisenberg, Alicia Hidalgo (2020) "A Toll-receptor map underlies structural brain plasticity" eLife DOI: 10.7554/eLife.52743 we frequently carry out outreach activities in which all team members are involved, and we explain the use of behaviroual assays in flies |
| First Year Of Impact | 2020 |
| Sector | Education,Healthcare,Pharmaceuticals and Medical Biotechnology |
| Description | BBSRC ISIS |
| Amount | £3,071 (GBP) |
| Funding ID | BB/P004997/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 07/2016 |
| End | 08/2016 |
| Title | New DeadEasy plugins to investigate brain plasticity |
| Description | We develop several new DeadEasy plugins, to count automatically cell number in the adult brain of the fruit-fly Drosophila: (1) DeadEasy Optic Lobe; (2) DeadEasy Central Brain; (3) DeadEasy Kenyon Cells. These were used in the publication Li et al 2020 eLife. |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2020 |
| Provided To Others? | Yes |
| Impact | This has only just been published, so impacts have not materialised yet. |
| URL | https://elifesciences.org/articles/52743 |
| Title | Novel DeadEasy plugins to investigate brain plasticity in Drosophila |
| Description | We have generated novel DeadEasy plugin to investigate brain plasticity in Drosophila. They enable us to automatically count cells throughout the brain: (1) DeadEasy Optic lobes for smaller cells; (2) DeadEasy Central Brain; (3) DeadEasy Kenyon Cells, for tightly packed cells. These plugins have been used in our publication Li et al 2020 eLife |
| Type Of Material | Computer model/algorithm |
| Year Produced | 2020 |
| Provided To Others? | Yes |
| Impact | This has only just been published so impact still unknown |
| URL | https://elifesciences.org/articles/52743 |
| Description | "Automatic quantification of cell number in the brain of Drosophila" |
| Organisation | University of Ibagué |
| Country | Colombia |
| Sector | Academic/University |
| PI Contribution | Contribution by my team: biological research data. |
| Collaborator Contribution | Contribution by collaborating team: software produced to analyse our data. |
| Impact | Recent publication in eLife: Guiyi Li, Manuel G Forero, Jill S Wentzell, Ilgim Durmus, Reinhard Wolf, Niki C Anthoney, Mieczyslaw Parker, Ruiying Jiang, Jacob Hasenauer, Nicholas James Strausfeld, Martin Heisenberg, Alicia Hidalgo (2020) "A Toll-receptor map underlies structural brain plasticity" eLife DOI: 10.7554/eLife.52743 |
| Start Year | 2018 |
| Description | Behavioural assays for structural plasticity and repair in the central nervous system of Drosophila |
| Organisation | University of Wurzburg |
| Country | Germany |
| Sector | Academic/University |
| PI Contribution | The aim of this collaboration was for my research team to learn from our collaborators about behaviour assays in fruit-flies. Once we have data ready for publication, we will offer them authorship in a joint paper. |
| Collaborator Contribution | We collaborated with Prof Martin Heisenberg and Dr Reinhard Wolf. One member of my team visited their lab to learn behaviour assays for fruit-flies. This helps our research by enriching the experimental approaches we can use to address our questions. |
| Impact | Behavioural analysis in flies. Outcomes not ready yet, work in progress. |
| Start Year | 2016 |
| Description | Collaboration with Professor Martin Heisenberg and Reinhard Wolf, University of Würzburg, Germany |
| Organisation | University of Wurzburg |
| Country | Germany |
| Sector | Academic/University |
| PI Contribution | Guiyi Li from my lab went to the lab of Professor Martin Heisenberg to learn to carry out behaviour experiments including: the Buridan arena essay, the optomotor response and learning and memory. She brought these techniques back to the lab, as well as some kit made in the Würzburg workshop. |
| Collaborator Contribution | Teaching staff from my lab to cary out some techniques, and providing us with kit to carry out those experiments. It is difficult to estimate the commercial value of the kit (hence the reason for not declaring it as an 'in kind' contribution, as they were designed and made by our collaborators' workshop, but they're extremely useful for our research. |
| Impact | We have just recently submitted an article for publication that resulted from this collaboration: Li et al (submitted) A Toll-receptor map underlies structural brain plasticity. |
| Start Year | 2016 |
| Title | DeadEasy Central Brain |
| Description | DeadEasy Central Brain counts automatically in vivo all the nuclei in the Drosophila adult central brain, labelled with histone-YFP. Will count other nuclei of similar size, stained with nuclear markers. |
| Type Of Technology | Software |
| Year Produced | 2020 |
| Impact | recently published in Li et al 2020 eLife |
| URL | https://elifesciences.org/articles/52743 |
| Title | DeadEasy Kenyon Cells |
| Description | DeadEasy Kenyon Cells counts automatically in vivo the tightly packed cells of the mushroom bodies of Drosophila, labelled with histone-YFP. Will count other similar nuclei. |
| Type Of Technology | Software |
| Year Produced | 2020 |
| Impact | recently published, impacts not materialised yet |
| URL | https://elifesciences.org/articles/52743 |
| Title | DeadEasy Optic Lobe |
| Description | DeadEasy Optic Lobes counts automatically the many and tiny cell nuclei labelled with Histone-YFP of the Drosophila adult optic lobes, in 3D, in vivo. It will count other cells of similar size, visible with fluorescent nuclear markers. |
| Type Of Technology | Software |
| Year Produced | 2020 |
| Impact | Published in Li et al 2020 eLife |
| URL | https://elifesciences.org/articles/52743 |