MRC DTG studentship: Origins of cancer-causing chromosonal translocations
Lead Research Organisation:
Babraham Institute
Department Name: UNLISTED
Abstract
Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.
Technical Summary
Chromosomal translocations lead to genes becoming fused together and are frequent causes of many types of cancer, yet these events are not well understood. We hypothesize that the manner by which genes are organized in the cell nucleus will impact how often two genes may form a chromosomal translocation. We have found that when they are active, genes are often found in close proximity in specialised nuclear compartments called transcription factories. This project aims to investigate whether chromosomal translocations can occur between genes when they are engaged at the same transcription factory.
Planned Impact
unavailable
People |
ORCID iD |
| Cameron Osborne (Principal Investigator) |
Publications
Mifsud B
(2015)
Mapping long-range promoter contacts in human cells with high-resolution capture Hi-C
in Nature Genetics
Osborne CS
(2011)
Meet the neighbours: tools to dissect nuclear structure and function.
in Briefings in functional genomics
Osborne CS
(2014)
Molecular pathways: transcription factories and chromosomal translocations.
in Clinical cancer research : an official journal of the American Association for Cancer Research
| Description | Bloodwise |
| Amount | £265,268 (GBP) |
| Organisation | Leukaemia and Lymphoma Research |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 03/2014 |
| End | 04/2017 |
| Description | Carry-on LLR grant |
| Amount | £97,302 (GBP) |
| Organisation | Leukaemia and Lymphoma Research |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | |
| Title | Capture Hi-C |
| Description | This method enables Hi-C chromosome conformation capture sequencing libraries to be enriched for the genomic interactions of special interest, such as those of gene promoters, to identify their long-range regulatory interactions to elements, such as enhancers, and to regions of the genome that are associated with disease. |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2014 |
| Provided To Others? | Yes |
| Impact | Several papers using this methodology have been published to date |
| Description | Capture Hi-C |
| Organisation | Agilent Technologies |
| Country | United States |
| Sector | Private |
| PI Contribution | We carried out the experimentation and development of the methodology. |
| Collaborator Contribution | Agilent provided custom designed Sureselect solution hybridisation selection arrays for use in the studies, as well as some NGS sequencing. |
| Impact | Resulted in the publication, Mifsud et al. 2015 Nature Genetics. |
| Start Year | 2011 |
| Description | Capture Hi-C |
| Organisation | Francis Crick Institute |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | In the project to use Capture Hi-C to examine the long-range promoter interactions across the genome, we carried out the wet-lab experimentation |
| Collaborator Contribution | The research group of our collaborator, Nick Luscombe, provided the bioinformatics analyses of the data we generated. |
| Impact | Publication of the study. Mifsud et al. 2015 Nature Genetics. |
| Start Year | 2011 |
| Title | CHROMOSOME CONFORMATION CAPTURE METHOD INCLUDING SELECTION AND ENRICHMENT STEPS |
| Description | The invention relates to a method for identifying nucleic acid segments which interact with a target nucleic acid segment by use of an isolating nucleic acid molecule, and to kits for use in said method. The invention also relates to a method of identifying one or more interacting nucleic acid segments that are indicative of a particular disease state. |
| IP Reference | WO2015033134 |
| Protection | Patent application published |
| Year Protection Granted | 2015 |
| Licensed | No |
| Impact | NA |