Systematic characterisation of epigenomes and transcriptomes during early mouse development

Lead Research Organisation: Babraham Institute
Department Name: UNLISTED

Abstract

Work in this Theme provides an unprecedented opportunity for this grouping of PIs and for the national and international scientific community to establish reference epigenomes for the key stages of epigenetic reprogramming and programming in germ cells, preimplantation embryos, and lineage commitment in early postimplantation embryos. This resource together with the development of an integrated epigenomics database will underpin the biological exploration of epigenetic reprogramming mechanisms, the unravelling of signalling principles that pattern the epigenome during development and ageing, and the understanding of how non-coding RNAs regulate the epigenome. This resource will become available to the national and international community through established high quality links (eg EU Network of Excellence, IHEC) and will be of great value not only for the epigenetics community, but also for developmental biologists, geneticists, reproductive and evolutionary biologists, stem cell researchers, and those working on the biology of ageing

Publications

10 25 50
 
Description This research theme aimed at mapping epigenetic information across the genome during key stages in the mammalian life-course, in particular the specification and development of germ cells and in the early embryo, during which major changes in epigenetic states occur and are necessary for cells to change from a germ cell fate to becoming totipotent, and then again to allow embryonic lineages to arise. Many epigenomes were determined across these stages, strongly enriching our knowledge in this important area for development and transgenerational epigenetic inheritance. We have pioneered low and single cell technologies, allowing studies of the transcriptome, methylome and chromatin heterogeneity, as well as highly-sensitive methods for detecting derivatives of 5-methylcystosine. These methods are proving transformative in scientific areas as diverse as oocyte epigenetics (where material is highly limited and improving the sensitivity of methods substantially reduces animal requirements), determination of lineage specificity during embryogenesis, and for investigating epigenetic heterogeneity in ageing.
Exploitation Route These findings have been taken up by the community as judged by research publications that build on our results
Sectors Healthcare,Pharmaceuticals and Medical Biotechnology

URL https://www.babraham.ac.uk/our-research/epigenetics
 
Description As part of the wider Babraham Institute strategy, across the course of this ISP we have developed a much broader public engagement strategy, undertaking large prestigious events including two Royal Society exhibits specifically showcasing our work (Epigenetics: DNA is not your destiny and Race Against the Ageing Clock), as well as presentations at local science fairs and engagement with local schools. We have also contributed substantially to transferable skills development, analytical thinking and training, mentoring and career development.
First Year Of Impact 2012
Sector Education,Healthcare
Impact Types Cultural,Societal,Economic

 
Description Babraham Institute Public Dialogue 2015
Geographic Reach National 
Policy Influence Type Citation in other policy documents
Impact Improved public understanding of biological sciences and the work of the Babraham Institute
URL http://www.babraham.ac.uk/get-involved/partnerships-page/public-dialogue
 
Description CTR Board of Managers (MH)
Geographic Reach Europe 
Policy Influence Type Influenced training of practitioners or researchers
Impact Member, Board of Managers of the Centre for Trophoblast Research, Cambridge assessing funding for targetted PhD studentship and Next Generation fellowship applciations and influencing science strategy within this research area.
 
Description Chair SAB Development and Genetics Unit Institut Curie
Geographic Reach Local/Municipal/Regional 
Policy Influence Type Participation in a advisory committee
Impact Probably the best unit of this type in France
 
Description Chair of SAB Centre for Trophoblast Research University of Cambridge
Geographic Reach National 
Policy Influence Type Participation in a advisory committee
Impact Has changed the perception of placenta and trophoblast in reproduction
 
Description European Research Council committee member
Geographic Reach Europe 
Policy Influence Type Participation in a advisory committee
 
Description H2020 EU-LIFE project "LIBRA", co-lead work package "Gender Equality in Research"
Geographic Reach Europe 
Policy Influence Type Influenced training of practitioners or researchers
Impact Consideration of gender effects in research, appreciation that there may be sex differences in cell lines, animal models and treatment regimes that ought to be taken into consideration when designing studies.
 
Description Research Advisory Board, Wellbeing of Women
Geographic Reach National 
Policy Influence Type Participation in advisory committee
Impact I served as member of the scientific advisory committee to the charity Wellbeing of Women, which reviews and makes decisions on grant funding in the general field of reproductive medicine. Some of the work funded through WoW has led to improvements in health care, novel diagnostic methods, etc.
 
Description Review panel member, DFG Clinical Research Unit (Germany) (MH)
Geographic Reach Europe 
Policy Influence Type Membership of a guideline committee
Impact Funding assessment for a major research unit on male infertility which includes improvement of patient treatment options and better patient assessment and stratification. Funding was recommended by teh panel and was implemented.
 
Description Wellcome Trust funding commitee
Geographic Reach National 
Policy Influence Type Participation in a advisory committee
 
Description EU BLUEPRINT
Amount £500,000 (GBP)
Funding ID 282510 
Organisation European Commission 
Department Seventh Framework Programme (FP7)
Sector Public
Country European Union (EU)
Start  
 
Description EU NoE EpiGeneSys
Amount £400,000 (GBP)
Funding ID 257082 
Organisation European Commission 
Sector Public
Country European Union (EU)
Start  
 
Description Epigenome patterning in oocytes and its legacies in the embryo
Amount £1,964,775 (GBP)
Funding ID MR/S000437/1 
Organisation Medical Research Council (MRC) 
Sector Academic/University
Country United Kingdom
Start 08/2018 
End 07/2023
 
Description MRC Collaboration Grant (deep sequencing in Epigenomics)
Amount £960,000 (GBP)
Funding ID G0801156 
Organisation Medical Research Council (MRC) 
Sector Academic/University
Country United Kingdom
Start  
 
Title High through-put single-cell DNA methylation profiling 
Description We have improved our previously published method of profiling DNA methylation genome-wide in single cells (Smallwood et al., Nat. Methods 2014), by implementing the method on a liquid handing robot (Clark et al. Nat. Protocols 2017), which is allowing much higher through-put applications. An extensive protocol is provided. 
Type Of Material Technology assay or reagent 
Year Produced 2017 
Provided To Others? Yes  
Impact Allowing much higher through-put applications. 
 
Title Single cell bisulfite sequencing 
Description Single cell method for methylome sequencing 
Type Of Material Technology assay or reagent 
Year Produced 2014 
Provided To Others? Yes  
Impact Widely quoted technique which is being implemented by other laboratories 
URL http://www.nature.com/nmeth/journal/v11/n8/full/nmeth.3035.html
 
Title Single cell combined RNA and bisulfite sequencing 
Description Method for sequencing of the transcriptome and methylome from the same single cell 
Type Of Material Technology assay or reagent 
Year Produced 2016 
Provided To Others? Yes  
Impact This has just been published 
URL http://www.nature.com/nmeth/journal/v13/n3/full/nmeth.3728.html
 
Title Single cell triple-omics 
Description Single cell triple-omics combining transcriptome, methylome, and chromatin accessibility from the same single cell. 
Type Of Material Technology assay or reagent 
Year Produced 2017 
Provided To Others? Yes  
Impact This method was just published. 
 
Title Single-cell BS-seq 
Description We developed a method to profile DNA methylation genome-wide in single cells, which has now been published (Smallwood et al., Nat. Methods 2014). This capability could have widespread application in assessing DNA methylation in rare cells (e.g., human gametes, preimplantation embryos), to assess DNA methylation heterogeneity amongst cells, which could be an important mechanism in developmental decisions, aging and pathological states, notably in cancer. 
Type Of Material Technology assay or reagent 
Year Produced 2014 
Provided To Others? Yes  
Impact The development of this method has led to the establishment of new collaborations for our group. It has also been a key component underpinning the award of a Wellcome Trust Strategic Award by a consortium headed by my colleague Prof. W. Reik. 
 
Title ChIP-seq profiles for Tet1 in TSCs (GSE109545) 
Description Chromatin binding profiles of Tet1 protein in trophoblast stem cells (TSCs) 
Type Of Material Database/Collection of data 
Year Produced 2018 
Provided To Others? Yes  
Impact Data allow to determine the functional role of Tet1 in TSCs 
 
Title Chromatin modifications during mouse oogenesis 
Description High quality native ChIP-seq datasets for histone modifications H3K4me3, H3K27ac, H3K27me3 during oocyte growth and fully grown oocytes in the mouse. ChIP-seq datasets for H3K4me3 in oocytes genetically deficient in DNA methylation (Dnmt3a/Dnmt3b nulls). ChIP-seq datasets for oocytes genetically deficient in the major H3K4 methyltransferase MLL2 (KMT2B). Published in Hanna et al. Nat. Struct. Mol. Biol. 2018 
Type Of Material Database/Collection of data 
Year Produced 2018 
Provided To Others? Yes  
Impact Recently released datasets, too early to identify impact outside of research group. 
URL https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE93941GSE93941
 
Title DNA methylation during mouse oogenesis 
Description Genome-wide DNA methylation datasets by RRBS and PBAT from size-selected mouse oocytes during the oocyte growth phase. Published in Gahurova et al. 2017 Epigenetics and Chromatin. 
Type Of Material Database/Collection of data 
Year Produced 2017 
Provided To Others? Yes  
Impact Only recently released, impact outside of research group too early to say. 
URL https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE86297
 
Title Gene expression during mouse oogenesis 
Description Deeply sequenced RNA-seq datasets from size-selected mouse oocytes during the oocyte growth phase. Published in Gahurova et al. 2017 Epigenetics and Chromatin. 
Type Of Material Database/Collection of data 
Year Produced 2017 
Provided To Others? Yes  
Impact Only recently released, too early to identify impacts beyond research group. 
URL https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE86297
 
Title Oocyte ChIP-seq datasets 
Description ChIP-seq datasets for selected histone modifications in mouse growing oocytes. DNA methylation profiles of oocytes lacking the histone demethylases KDM1A, KDM1B. As reported in Stewart et al., 2015 Genes and Development, 29, 2449-2462 
Type Of Material Database/Collection of data 
Year Produced 2015 
Provided To Others? Yes  
Impact Too soon to define notable impacts, as the associated manuscript was published in Genes and Development in December 2015 
URL http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE74549
 
Title Oocyte transcriptome, mouse 
Description Deep strand-specific RNA-seq to establish in-depth transcriptome of mouse oocytes, as reported in Veselovska et al., 2015 Genome Biology 16, 209 
Type Of Material Database/Collection of data 
Year Produced 2015 
Provided To Others? Yes  
Impact The publication in Genome Biology associated with these datasets has already attracted >30 citations, suggesting a good level of uptake of the data by the research community. 
URL http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE70116
 
Title Stem Cell methylomes 
Description DNA methylation (meDIP)-seq profiling of ESCs, TSCs, XEN cells and EpiSCs 
Type Of Material Database/Collection of data 
Provided To Others? No  
Impact Data were deposited in publicly accessible databases. Data will be useful to many other researchers for meta-analyses. 
 
Title Transcriptome datasets of reproductive tissues in ageing females (GSE98901) 
Description RNA-seq datasets of trophoblast, endometrium and deciduae of mouse conceptuses developed in young versus aged females 
Type Of Material Database/Collection of data 
Year Produced 2017 
Provided To Others? Yes  
Impact Significant changes imposed by maternal age on expression profiles of endoemtrial tissue and decidual development. Work shows the impact of the older female on reproductive success, unrelated to oocyte fitness. 
 
Title Webtool for integration of high-throughput datasets and their rapid analysis 
Description Generation of a webtool allowing rapid data analysis of high-througput sequencing datasets. 
Type Of Material Computer model/algorithm 
Year Produced 2018 
Provided To Others? No  
Impact Webtool allows to upload and integrate multiple datasets of different nature (such as ChIP-seq, RNA-seq, DNA methyaltion profiles) and allows to perform rapid multivariate analyses on them to find co-occpuancy, mutual exclusion etc 
 
Description Active Motif DNA modification antibody 
Organisation Active Motif
Country United States 
Sector Private 
PI Contribution Commercialisation of DNA modification antibodies we have made
Collaborator Contribution Commercialisation of DNA modification antibodies we have made
Impact Commercially available antibodies
Start Year 2016
 
Description Analysis of Mll2-deficient oocytes 
Organisation Technical University of Dresden
Department Biotechnology Center
Country Germany 
Sector Academic/University 
PI Contribution We framed the biological question and were responsible for experimental design and data analysis. We performed DNA methylation and histone modification analysis of oocytes conditionally deleted for Mll2 provided by the partner.
Collaborator Contribution Provided mice with conditional knock-out allele of Mll2 and Mll2-deficient oocytes.
Impact Joint publication: Hanna et al., Nat. Struct. Mol. Biol. 2018. Deposited sequence datasets at NCBI GEO: GSE93941.
Start Year 2013
 
Description Bioinformatic analysis of oocyte ChIP-seq data 
Organisation Helmholtz Zentrum München
Department Institute of Computational Biology
Country Germany 
Sector Private 
PI Contribution We provided ChIP-seq datasets for various histone modifications in mouse wild-type and genetic knock-out oocytes to enable partner to test new informatics tools they had developed.
Collaborator Contribution The partner provided new informatics methods, based on multivariate Hidden-Markov Models, to enable analysis of multiple ChIP-seq datasets, with focus on multivariate comparisons.
Impact Joint publication: Hanna et al., Nat. Struct. Mol. Biol. 2018. Deposited sequence datasets at NCBI GEO: GSE93941. Hosted visiting bioinformatics PhD student from collaborating lab. Inter-disciplinarity: partner provides modelling and informatics expertise, our group provides biological datasets.
Start Year 2016
 
Description Consultancy Cambridge Epigenetix 
Organisation Cambridge Epigenetix
PI Contribution I am a consultant for the company
Collaborator Contribution None at present
Impact Oxidative bisulfite sequencing
Start Year 2012
 
Description DNA methylation analysis in oocytes genetically deficient for Kdm1a (Lsd1) and Kdm1b (Aof1) 
Organisation University of Texas
Department M. D. Anderson Cancer Center
Country United States 
Sector Academic/University 
PI Contribution We were responsible for framing the project and experimental design and performed genome-wide DNA methylation analysis in oocytes genetically deficient in the H3K4me2 demethylases KDM1A or KDM1B, provided by the collaborating group.
Collaborator Contribution The partner provided oocytes from mice genetically deficient in Kdm1a or Kdm1b; provided RNA-seq datasets for these oocytes.
Impact Joint publications Stewart et al., 2015 Genes & Dev; Gahurova et al. 2017 Epigenetics & Chromatin. Deposited sequence datasets at NCBI GEO: GSE73803 and GSE74549.
Start Year 2011
 
Description DNA methylation analysis in oocytes genetically deficient in Hira 
Organisation Medical Research Council (MRC)
Department MRC Clinical Sciences Centre (CSC)
Country United Kingdom 
Sector Academic/University 
PI Contribution We performed single-cell, genome-wide bisulphite sequencing of oocytes deficient in H3.3 chaperone HIRA, provided by the collaborating group; using our newly established single-cell BS-seq method (Smallwood et al., 2014 Nat. Meths).
Collaborator Contribution The partner was responsible for framing the project and experimental design and provided mouse oocytes conditionally deleted in Hira.
Impact Joint publication Nashun et al. 2015 Mol. Cell. Deposited sequence datasets at NCBI GSE66629.
Start Year 2014
 
Description DNA methylation dynamics in single cells 
Organisation University of Cambridge
Department Gurdon Institute
Country United Kingdom 
Sector Academic/University 
PI Contribution Contributed project design and single cell sequencing data
Collaborator Contribution Contributed project design and mathematical models
Impact First paper is under submission
Start Year 2014
 
Description Deciduoma 
Organisation National Institute of Environmental Health Sciences
Department Reproductive and Developmental Biology Laboratory
Country United States 
Sector Public 
PI Contribution Characterisation of reproductive defects in older females
Collaborator Contribution Decidualisation model to assess the effect in the absence of an implanting embryo
Impact Collaborative publication (PMID: 28874785)
Start Year 2016
 
Description Donal O'Carrol 
Organisation University of Edinburgh
Country United Kingdom 
Sector Academic/University 
PI Contribution Helped with methylome sequencing and computational work.
Collaborator Contribution Conceived and conducted the study.
Impact Publication Vasiliauskaite et al 2017. Developmental biologists, epigeneticists, computational biologists.
Start Year 2016
 
Description Gastrulation team 
Organisation University of Cambridge
Country United Kingdom 
Sector Academic/University 
PI Contribution Conceived of the project and lead partner. Contributing single cell multi-omics and embryology.
Collaborator Contribution Helped to conceive of the project and several experimental and computational contributions.
Impact First publication Mohammed et al 2017. Experimental embryology, single cell biology, computation, modelling, epigenetics.
Start Year 2014
 
Description Guiseppe Lupo 
Organisation Sapienza University of Rome
Country Italy 
Sector Academic/University 
PI Contribution We have profiled the epigenome and transcriptome of aged neural stem cells. We have also hosted GL in our laboratory over two summers and provided training in epigenomics techniques.
Collaborator Contribution Our partners derived and characterised young and aged neural stem cells, provide expertise in neural biology, and have given training to us in neural stem cell cultures.
Impact Manuscript in preparation.
Start Year 2014
 
Description Human Cell Atlas 
Organisation Broad Institute
Country United States 
Sector Charity/Non Profit 
PI Contribution Contributed to formulating the white paper of the Human Cell Atlas.
Collaborator Contribution Conceived the project and brought the international consortium together.
Impact The white paper was published Regev et al 2017. It involves single cell biologists and computational and modelling scientists.
Start Year 2016
 
Description Jenny Nichols, Austin Smith 
Organisation University of Cambridge
Country United Kingdom 
Sector Academic/University 
PI Contribution Contributed to conceiving studies. Epigenetic profiling and analysis.
Collaborator Contribution Conceived and conducted studies.
Impact Publications Kalkan et al 2017, Guo et al 2017. Involves embryologists, stem cell biologists, computational biologists, epigeneticists.
Start Year 2016
 
Description Millipore DNA modification antibodies 
Organisation Merck
Department MilliporeSigma
Country United States 
Sector Private 
PI Contribution Commercialisation of DNA modification antibodies we have made
Collaborator Contribution Commercialisation of DNA modification antibodies we have made
Impact DNA modification antibodies
Start Year 2011
 
Description Sanger Single Cell Genomics Centre 
Organisation The Wellcome Trust Sanger Institute
Country United Kingdom 
Sector Charity/Non Profit 
PI Contribution Helped to establish the Single Cell Genomics Centre at Sanger/EBI
Collaborator Contribution A number of Sanger and EBI PIs were involved
Impact Genomics, transcriptomics, epigenomics, computational biology
Start Year 2013
 
Description XIMBIO DNA modification antibodies 
Organisation Cancer Research Technology (CRT)
Country United Kingdom 
Sector Private 
PI Contribution Commercialisation of DNA modification antibodies we have made
Collaborator Contribution Commercialisation of DNA modification antibodies we have made
Impact DNA modification antibodies
Start Year 2016
 
Description uNK cells 
Organisation University of Cambridge
Country United Kingdom 
Sector Academic/University 
PI Contribution Characterisaiton of reproductive problems in older females.
Collaborator Contribution Analysis of immune repertoire of implantation sites depending on maternal age.
Impact Collaborative publication (PMID: 28874785).
Start Year 2016
 
Description Babraham Schools Day 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Schools
Results and Impact Annual event in which local GCSE and A-level students are hosted in my group and many other groups at the Institute for a day of practical demonstrations.
Year(s) Of Engagement Activity Pre-2006,2006,2007,2008,
 
Description Cambridge Science Festival 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Exhibit about Babraham's research at Cambridge Science Festival
Year(s) Of Engagement Activity 2015
 
Description Cambridge Science Festival: Molecular Explorers, Cambridge (UK) 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Exhibit at Cambridge Science Festival, engaging with visitors at stall explaining the science and advances made in the area, answering questions and discussing outstanding research goals
Year(s) Of Engagement Activity 2017
 
Description Cold Spring Harbor Laboratory meeting: Stem Cell Biology, New York (USA) (VPG, CS) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact Participation in confenrence, poster presentation by both post-doctoral reserachers involved in this activity (VPG and CS) and engagement in discussions and broadening of scientific horizon
Year(s) Of Engagement Activity 2017
 
Description EMBL Mammalian Genetics and Genomics Conference (MH) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Invited speaker at AMBL Conference Mammalian genetic and Genomic, Heidelberg, Germany
Year(s) Of Engagement Activity 2017
 
Description Genetics Society and BSDB Spring Meeting, Warwick, UK (MH) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Invited speaker at annual spring meeting of the Genetics Society and British Society for Developmental Biology (BSDB) in Warwick, UK (2017)
Year(s) Of Engagement Activity 2017
 
Description IFPA Meeting, Manchester, UK (MH) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Invited talk at IFPA Meeting held in Manchester, UK (2017)
Year(s) Of Engagement Activity 2017
 
Description ISSHP Meeting, Berlin, Germany (MH) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Invited talk at ISSHP Meeting held in Berlin, Germany (2017)
Year(s) Of Engagement Activity 2017
 
Description Keynote speaker, CTR Anniversary Meeting, Cambridge, UK (MH) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact Keynote lecture at 10th Anniversary Meeting of the Centre for Trophoblast Research, University of Cambridge (2017)
Year(s) Of Engagement Activity 2017
 
Description Member, Board of Managers at Centre for Trophoblast Research 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? Yes
Geographic Reach International
Primary Audience Supporters
Results and Impact As member of the Board of Managers at the CTR in Cambridge we make decisions on the award of PhD scholarships, postdoctoral fellowships and direct the general focal points of local research activity in the field of placental biology.

PhD students admitted to the University of Cambridge; postdoctoral researchers' career path enhanced
Year(s) Of Engagement Activity 2013,2014,2015,2016
 
Description Podcast with Dr Kat Arney on the impact of placentation and maternal age on reproductive outcome 
Form Of Engagement Activity A broadcast e.g. TV/radio/film/podcast (other than news/press)
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Podcast as descibed above
Year(s) Of Engagement Activity 2017
 
Description Postgraduate course Epigenetics in Reproductive Biology, University of Murcia, Spain (VPG) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Postgraduate students
Results and Impact Invited talk and workshop participation as part of postgraduate course on reproductive epigenetics
Year(s) Of Engagement Activity 2016,2017
 
Description Press interview on Maternal methylation inheritance 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Media (as a channel to the public)
Results and Impact Co-interviewee which formed part of a televised clip reporting on a recent publication and its societal/public impact.
Year(s) Of Engagement Activity 2016
 
Description Press release - Keeping eggs fresh with epigenetics 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Media (as a channel to the public)
Results and Impact Press release from Babraham to accompany publication of paper 'MLL2 conveys transcription-independent H3K4me3 in the oocyte', by Hanna et al. Nat. Struct. Mol. Biol. 2018. Picked up by 9 news outlets and blogs.
Year(s) Of Engagement Activity 2018
URL https://www.babraham.ac.uk/news/2018/01/keeping-egg-cells-fresh-with-epigenetics
 
Description Radio interview DeutschlandFunk 
Form Of Engagement Activity A broadcast e.g. TV/radio/film/podcast (other than news/press)
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Radio Interview for DeutschlandFunk on the impact of maternal age on pregnancy success, irrespective of the increased risk of defects in the egg
Year(s) Of Engagement Activity 2017
URL http://www.deutschlandfunk.de/alter-des-uterus-entscheidend-junges-glueck-in-jungen.676.de.html?dram...
 
Description Royal Society Partnering Award - CS + LW, NM, SC 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Successful application for Royal Society Partnering Award. Poster 6 selected 6th form students over the course of 1 week and conducted in-depth research project. Data analysis followed at the pupils' school directly.
Year(s) Of Engagement Activity 2016,2017
 
Description STEM teacher visit 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact As part of a week-long visit to the Institute, my group hosted 3 biology A-level teachers from local schools for involvement in practical work in the lab over a 2-day period.
Year(s) Of Engagement Activity 2016
 
Description School's Day 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? Yes
Geographic Reach Local
Primary Audience Schools
Results and Impact provided basic explanation of early development and the importance of the placenta for growth of a baby; hands-on experience for students in staining and microscopic analysis of a mouse placenta

rose awareness of research activities and importance of basic research to understand common pregnancy-associated diseases
Year(s) Of Engagement Activity 2006,2007,2008,2009,2010
 
Description Schools Day 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Schools
Results and Impact Groups of 5-6 6th-form students take part in a one-day visit to 2 research groups at the Babraham Institute and conduct two small research experiments during that day.
Year(s) Of Engagement Activity 2017,2018
 
Description Schools Day 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Project design, scientific background induction and practical supervision for 6th form students as well as teachers during Annual Schools Day.
Year(s) Of Engagement Activity 2015,2016,2017
 
Description The Ageing Cell Conference, Cambridge, UK (MH) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact Invited talk at The Ageing Cell Conference held in Cambridge, UK (2017)
Year(s) Of Engagement Activity 2017
 
Description Twilight teacher training event, Bioscience lite-CRISPR-Cas9 genome editing- technology, applications and implication, Cambridge (UK) (VPG) 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Schools
Results and Impact Talk and Q&A session with high school teachers to update and inform them on state-of-the-art genome editing tools
Year(s) Of Engagement Activity 2017