To map the organisation of nutrient signalling pathways

Lead Research Organisation: Babraham Institute
Department Name: UNLISTED

Abstract

We propose to begin to characterize the responses of freshly-prepared, collagenase-liberated isolated mouse hepatocytes to a range of specific nutrient challenges, that cannot be applied in vivo, in terms of their effect on the class I & III PI3K pathways to mTor, mTor activity, the intiation of autophagy and ROS production. So that in the second half of the project we can look at aged and diet-restricted animals. We aim to publish our first major study indicating that amino acids controlling mTor activity are taken-up by macro-pinocytosis and to have advanced our work addressing the nature of the Ca2+ signals controlling mTor at lysosomes near to a point of submission. We will have tested several amino acid analogues for their effect on mTORC1 activation and then image their distribution within cells. A project following-up the confirmed hits from our genome-wide screens for suppressors and activators of vps34 complexes in autophagy. This work will focus on specific hypotheses arising from that work using a combination of molecular, cell biology and systems approaches to assemble subsets of hits into meaningful regulatory networks. Within that project we aim to have isolated the subset of hits that appear to affect mTORC1 localization and activity and begun to generate a signalling diagram leading from starvation to autophagy. We will have begun to analyse the products of DAz-2 derivatised cell lysates to reveal the complexity of the samples we will be attempting to analyse. Following on from our new data we will define the activation status of ERK1/2, p38, MNK1/2 and eIF4E during nutrient starvation and during acute and chronic mTOR inhibition in primary human fibroblasts and assess their activation in young and aged fibroblasts. This will be a pre-requisite to gene expression studies and analysis of young and aged samples.

Publications

10 25 50

publication icon
Bulley SJ (2016) In B cells, phosphatidylinositol 5-phosphate 4-kinase-a synthesizes PI(4,5)P2 to impact mTORC2 and Akt signaling. in Proceedings of the National Academy of Sciences of the United States of America

 
Description mTOR is a master regulator of nutrient status. In organisms as diverse as worms, flies, mice and men it coordinates how cells respond to changes in nutrient availability. In addition, drugs that inhibit mTOR can mimic nutrient starvation and increase lifespan in several organisms and can make longer lived organisms healthier (increasing their healthspan).
We discovered how mTOR, an important kinase that regulates autophagy, traffics on and off lysosomes in response to amino acids.
We also discovered how the earliest autophagy intermediate looks like by combining live imaging and super resolution microscopy.
In addition we have discovered one mechanism by which cells are able to adapt to long-term inhibition of mTOR. This may be relevant to models of lifespan/healthspan extension and also to possible therapeutic uses of mTOR inhibitors.
Exploitation Route The dynamics of the processes that we described can be analyzed in neuronal cells undergoing neurodegeneration, or in cells as they age, in order to assay the benefits of potential drugs. Indeed, understanding how cells adapt to long-term mTOR inhibition will be important to pharmaceutical companies seeking to develop mTOR inhibitors of treatment of disease
Sectors Education,Healthcare,Pharmaceuticals and Medical Biotechnology

 
Description Reagents and assays developed have been used to set up a screening platform for identification of novel autophagy modulators. This has been funded by the Institute and is using a chemical library provided by MRC-T. We have also generated immortalised human cell lines that have adapted to chronic mTOR inhibition (which mimics nutrient starvation) and investigated the underlying mechanisms of adaptation. These cell lines have been made available to other academics and industrial collaborators. For example, AstraZeneca are developing mTOR inhibitors for the treatment of malignancy and are interested in how cells adapt and acquires resistance to such drugs and this will help them to maximise drug efficacy and identify patient populations most likely to respond.
First Year Of Impact 2014
Sector Education,Healthcare,Pharmaceuticals and Medical Biotechnology
Impact Types Economic

 
Description Babraham Institute Representative on Translational Research at EU Life, a consortium of EU Life Sciences Institutes
Geographic Reach Europe 
Policy Influence Type Membership of a guideline committee
URL http://eu-life.eu/
 
Description Member of Scientific Advisory Board of Cancer Research Technology Discovery Lab
Geographic Reach National 
Policy Influence Type Participation in a advisory committee
 
Description Membership of Scientific Advisory Board for Cancer Research Technology
Geographic Reach National 
Policy Influence Type Participation in a advisory committee
 
Description Scientific Advisory Board for CRUK Programme Grant awarded to Northern Institute for Cancer Research, Univeristy of Newcastle
Geographic Reach Local/Municipal/Regional 
Policy Influence Type Participation in a advisory committee
 
Description BBSRC Response mode project grant
Amount £322,649 (GBP)
Funding ID BB/L008793/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 12/2013 
End 08/2017
 
Description BBSRC Response mode project grant
Amount £327,000 (GBP)
Funding ID BB/P007015/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 06/2017 
End 05/2020
 
Title IKKalpha and IKKbeta KO cell lines 
Description We have generated HCT116 cell lines lacking one or other or both (DKO) of the critical NFkB activating protein kinases IKKalpha or IKKbeta using CRISPR/Cas9 gene targeting. 
Type Of Material Cell line 
Provided To Others? No  
Impact Too early for impacts; cells still being charatcerised. These will be made available to the community in the future 
 
Title Inducible DYRK1B cells 
Description HEK293 cells lines engineered to exhibit Tet-inducible expression of the DYRK1B protein kinase 
Type Of Material Cell line 
Provided To Others? No  
Impact New knowledge of the biological function of the DYRK1B protein kinase, including new substrates. New research papers New collaborations 
 
Title Inducible DYRK2 cell line 
Description HEK293 cells lines engineered to exhibit Tet-inducible expression of the DYRK2 protein kinase 
Type Of Material Cell line 
Provided To Others? No  
Impact New knowledge of DYRK2 Identification of new substrates of DYRK2 
 
Title Pathway analysis 
Description Development of pathway analysis allowing for identification of enzyme activities modulated by cell stimulation, infection or metabolic change utilising novel bioinformatics methods. 
Type Of Material Model of mechanisms or symptoms - mammalian in vivo 
Year Produced 2017 
Provided To Others? Yes  
Impact Identification of potential therapeutic targets to treat infection. 
 
Title mTORi resistant cell lines 
Description We have generated human cell lines (cancer cells and primary human fibroblasts) with adaptive resistance to the mTOR inhibitors rapamycin and AZD8055 
Type Of Material Cell line 
Year Produced 2012 
Provided To Others? Yes  
Impact Two papers have been published and this has provided insight in to likely mechanisms by which cancer cells may adapt and acquire resistance to mTOR inhibitors 
 
Title DUB RNAi screen 
Description In collaboration with MISSION Therapeutics we have undertaken an RNAi screen with human deubiquitylating enzymes (DUBs) to identify those DUBs that confer protection against ERK pathway, mTOR inhibition or PERK inhibition Datat is currently being analysed before candidates are selected for further characterisation 
Type Of Material Database/Collection of data 
Year Produced 2017 
Provided To Others? Yes  
Impact Too early for impacts This data will be made available to the community in the future 
 
Title DYRK1B phosphoproteomics data set 
Description Using HEK293 cells exhibiting inducible expression of the DYRK1B protein kinase (HD1B cells) we have performed Phospho-SILAC mess spectrometry to identify DYRK1B-inducible phosphoproteins. Some of these turn out to be direct DYRK1B substrates 
Type Of Material Database/Collection of data 
Year Produced 2017 
Provided To Others? No  
Impact This data set has allowed us to identify new DYRK1B substrates and so has provided new insights into the function of this protein kinases in controlling gene expression and autophagy. The dataset will ultimately be released and freely available when the first mansucript is published 
 
Title DYRK2 phosphoproteomics dataset 
Description Using HEK293 cells exhibiting inducible expression of the DYRK2 protein kinase (HD2 cells) we have performed Phospho-SILAC mess spectrometry to identify DYRK2-inducible phosphoproteins. Some of these turn out to be direct DYRK2 substrates 
Type Of Material Database/Collection of data 
Year Produced 2017 
Provided To Others? No  
Impact This dataset has allowed us to identify DYRK2-inducible phosphoproteins, including new substrates, providing new insghts into the function of the DYRK2 protein in gene expression, autophagy/proteostasis and cell motility 
 
Title IKKa, IKKb and IKK DKO gene sets 
Description RNAseq data sets from human colorectal epithelial cell lines in which IKKa, IKKb or both IKKs have been deleted by CRISPR/Cas9. Both basal and TNFalpha-induced conditions were employed so that we can define the contribution of IKKs to basal and cytokine-induced gene expression. 
Type Of Material Database/Collection of data 
Year Produced 2017 
Provided To Others? No  
Impact The IKKs are the kinases that drive activation of the NFkappaB (NFkB) transcription factor. This is the first instance of knockout of the IKKs in human cells. We have confirmed the expression of known NFkB target genes but also identified an array of novel IKK-dependent genes that are candidate NFkB gene targets. Some of these have roles in autophagy, protein turnover, protein trafficking, cell motility and inflammation. This data will be made available freely when the first manuscript is published. 
 
Description AstraZeneca mTOR 
Organisation AstraZeneca
Country United Kingdom 
Sector Private 
PI Contribution Inhibitors of mTOR promote healthy lifespan extension in model organisms by promoting autophagy and are also undergoing evaluation as a treatment for cancer. However, complete mTOR inhibition is not compatible with life. We are interested in how the mTOR pathway is remodelled during chronic exposure to mTOR inhibitors. We have generated cells that are resistant to mTOR inhibitors and examined the mTOR pathway to identify changes that underpin resistance.
Collaborator Contribution Our partners at AstraZeneca part funded a BBSRC PhD student to do this work, provided large quantities of mTOR inhibtor as an 'in kind' contribution and performed next gen sequencing and other analyses of resistant cells to probe for mechanisms of resistance
Impact The PhD student successfully completed their PhD, two papers have been published and additional work is ongoing.
Start Year 2011
 
Description Di Christofano_PIP3_mouse_Thyroid 
Organisation Albert Einstein College of Medicine
Country United States 
Sector Academic/University 
PI Contribution Technical expertise: we measured PIP3 by mass spectrometry in their mouse thyroid biopsies
Collaborator Contribution They provided the main intellectual input and the mouse thyroid samples.
Impact Orlacchio A, Ranieri M, Brave M, Arciuch VA, Forde T, De Martino D..... Di Cristofano A, (2017). SGK1 Is a Critical Component of an AKT-Independent Pathway Essential for PI3K-Mediated Tumor Development and Maintenance.. Cancer research, 77 (24), pp. 6914-6926
Start Year 2016
 
Description Divecha 
Organisation University of Southampton
Country United Kingdom 
Sector Academic/University 
PI Contribution Measurement of phosphoinositides. GM mouse projects progressed.
Collaborator Contribution Preparation of cell extracts
Impact Multi-disciplinary, we do lipidomics and mouse work. They do cell line experiments and cancer related experiments
Start Year 2012
 
Description Divecha - phosphoinositides 
Organisation University of Southampton
Department Primary Care and Population Sciences
Country United Kingdom 
Sector Academic/University 
PI Contribution expertise, reagents, measurements of phosphoinositides by mass spectrometry
Collaborator Contribution Intellectual framework for study, biological samples
Impact Stijf-Bultsma et al (2015) Mol Cell. 58(3):453-67. doi: 10.1016/j.molcel.2015.03.009. Jones et al (2015) FEBS 281(16):3591-608. doi: 10.1111/febs.12879.
 
Description Doreen Cantrell 
Organisation University of Dundee
Country United Kingdom 
Sector Academic/University 
PI Contribution Measurements of phosphoinositides
Collaborator Contribution Samples of lymphocytes
Impact Publications that are fully reported
Start Year 2014
 
Description Herve Gillou 
Organisation French National Institute of Agricultural Research
Department INRA laboratory Toxalim
Country France 
Sector Public 
PI Contribution Measurements of Phosphosphoinositides
Collaborator Contribution Preparation of cell samples, dietary manipulation of mice, eg high fat diet
Impact Multi-discipllinary, We perform lipidomics , Toulouse group deal with mouse models and diet manipulation
Start Year 2013
 
Description Irvine_PI5P4K alpha 
Organisation University of Cambridge
Department Department of Pathology
Country United Kingdom 
Sector Academic/University 
PI Contribution We measured phosphoinositides by mass spectrometry
Collaborator Contribution They designed the study and provided biological material
Impact Bulley SJ, Droubi A, Clarke JH, Anderson KE, Stephens LR, Hawkins PT, Irvine RF. Proc Natl Acad Sci U S A. 2016 Sep 20;113(38):10571-6. doi: 10.1073/pnas.1522478113.
Start Year 2014
 
Description Mitophagy in human patients 
Organisation University of Oxford
Country United Kingdom 
Sector Academic/University 
PI Contribution Examining human fibroblasts from patients with mitochondrial dysfunction to identify those with mitophagy alterations
Collaborator Contribution Provision of patient fibroblasts and patient histories
Impact still in progress
Start Year 2018
 
Description Parsons - PREX 
Organisation Columbia University Medical Center
Country United States 
Sector Academic/University 
PI Contribution Analysis of phosphoinositides by mass spectrometry
Collaborator Contribution Intellectual frame work for study and biological material
Impact Hodakoski et al (2014) Proc Natl Acad Sci U S A. 111(1):155-60. doi: 10.1073/pnas.1213773111. Epub 2013 Dec 23.
Start Year 2012
 
Description Roger Williams 
Organisation Medical Research Council (MRC)
Department MRC Laboratory of Molecular Biology (LMB)
Country United Kingdom 
Sector Public 
PI Contribution Exchange of reagents and cell lines
Collaborator Contribution Exchange of reagents and cell lines
Impact Publications and grants
Start Year 2012
 
Description Synuclein clearance 
Organisation National University of Cordoba
Country Argentina 
Sector Academic/University 
PI Contribution Hosted student to study clearance of synuclein fibers by autohagy
Collaborator Contribution Model system
Impact publication
Start Year 2017
 
Description Tamotsu 
Organisation Osaka University
Country Japan 
Sector Academic/University 
PI Contribution Papers together
Collaborator Contribution Papers together
Impact Published 2 papers together
Start Year 2009
 
Description Van haesebroeck - PI3K 
Organisation University College London
Department UCL Cancer Institute
Country United Kingdom 
Sector Academic/University 
PI Contribution Intellectual input, reagents and mass spectrometric analysis of phosphoinositides
Collaborator Contribution Intellectual input, biological samples. Wide ranging collaboration covering several projects.
Impact Alliouachene et al (2015) Cell Rep. 13(9):1881-94. doi: 10.1016/j.celrep.2015.10.052 Gyori et al (2014) Arthritis Rheumatol. 66(8):2210-21. doi: 10.1002/art.38660. Kulkarni et al (2011) Sci Signal. 4(168):ra23. doi: 10.1126/scisignal.2001617.
Start Year 2009
 
Description Volker Haucke 
Organisation Leibniz Association
Department Leibniz-Institute for Molecular Pharmacology
Country Germany 
Sector Public 
PI Contribution Measurements of phopshoinositides and advice about assays for inostiol phosphates
Collaborator Contribution They have established assays of intracellualr trafficking using high content imaging technology and to measure mTOR1 distribution and roles.
Impact Multi-disciplinary, high content imaging, mass spectrometry, biochemisty
Start Year 2015
 
Description p62 phosphorylation 
Organisation University of Tromso
Department Department of Medical Biology
Country Norway 
Sector Academic/University 
PI Contribution Sharing of data; discussion of new experimental directions; experiments We have identified p62/SQSTM1 as a novel substrate of DYRK1B and DYRK2. We are investigating the role of this phosphorylation on p62 functions in proteostasis, stress responses and nutrient signalling
Collaborator Contribution Terje Johansen's lab will share reagents and expertise and perform specific experiments to define the interaction between DYRKs and p62
Impact Too soon for any specific outputs as the collaboration has only just started. However, i have visited the University of Tromso and presented two Lectures as part of a PhD training course
Start Year 2017
 
Title Identification of Deubiquitylating enzymes that arbitrate cell death in response ot ERK pathway or mTOR inhibition 
Description In collaboration with MISSION Therapeutics the PhD student has screened and identified DUBs that determine whether ERK pathway or mTOR inhibition leads ot cell death or not. These might be potential drug targets 
IP Reference  
Protection Protection not required
Year Protection Granted 2017
Licensed Commercial In Confidence
Impact Too early for direct impacts. Further validation and characterisation required
 
Title Identification of genes/enzymes driving resistance to mTOR inhibitors 
Description Identification of genes/enzymes driving acquired resistance to mTOR inhibitors - these are possibly druggable targets for overcoming acquired resistance 
IP Reference  
Protection Protection not required
Year Protection Granted
Licensed Yes
Impact Too early to say but these genes/enzymes are possibly druggable targets for overcoming acquired resistance
 
Title SBpipe 
Description This package contains a collection of pipelines for dynamic modelling of biological systems. It aims to automate common processes and speed up productivity for tasks such as model simulation, single and double parameter scan, and parameter estimation. 
Type Of Technology Software 
Year Produced 2017 
Open Source License? Yes  
Impact Streamlined model identifiability and parameter estimation in systems biology modelling. 
URL https://pdp10.github.io/sbpipe/
 
Description Cambridge Enterprise & Technology Club 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Professional Practitioners
Results and Impact Talk on autophagy and degenerative diseases
Year(s) Of Engagement Activity 2016
URL http://www.cetc.info/degenerative-diseases/
 
Description Cambridge Hellenic Learned Society 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Professional Practitioners
Results and Impact President of Society of Greek and Cypriot academics in Cambridge, charged with organizing events with well-known speakers in the fields of Science of Humanities and addressed to a lay/educated audience. So far we have organized 7 such events with average attendance of 90 people.
Year(s) Of Engagement Activity 2014,2015,2016
URL https://camlearnhelsoc.wordpress.com/
 
Description Computational Modeling in Biology Network 
Form Of Engagement Activity Engagement focused website, blog or social media channel
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I created COMBINE to coordinate the development of standards in computational systems biology. The initiative includes several workshops a year (discussion of future standards, implementation of current standards, and end-user training), diffusion lists, social media dissemination.
Year(s) Of Engagement Activity 2011,2012,2013,2014,2015
URL http://co.mbine.org
 
Description Deep dive autophagy discussion 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact Provided expert opinion on whether autophagy is a good investment area
Year(s) Of Engagement Activity 2019
 
Description Development strategy council 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact Part of an international panel of experts convened by the Greek Economic Ministry to help develop Biotech industries in Greece
Year(s) Of Engagement Activity 2017
 
Description In conversation with the Babraham Institute - part of Cambridge Science Festival 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact 1:1 dialogue with general public (open invitation but registration required for numbers).
Evening reception in which we explain our science and answer questions
Part of a programme of events for the annual Cambridge Science Festival
Year(s) Of Engagement Activity 2017
 
Description Interview with PBS USA 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Media (as a channel to the public)
Results and Impact Interviewed about effect of Brexit upon UK science and innovation
Year(s) Of Engagement Activity 2017
 
Description Invited lecturer to international meetings (average 2-3 per year) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact promoted discussions, collaborations

scientific collaborations, joint grants and publications
Year(s) Of Engagement Activity Pre-2006,2006,2007,2008,
 
Description Invited lecturer to international meetings (average 2-3 per year) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Generated discussion, exchange of ideas and collaborations
Year(s) Of Engagement Activity Pre-2006,2006,2007,2008,
 
Description Invited talk at scientific meetings 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Invited talks at international meetings in Japan (June 2017), Finland (September 2017), Croatia (September 2017), Argentina (November 2017)
Year(s) Of Engagement Activity 2017
 
Description Lipidomics workshops and lipid databasing, standards and nomenclature 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Establishing internationally agreed format for reporting lipid mass spectrometry data, agreeing structures for archiving of lipidomics data, international links, transfer of LIPID MAPS website and databasing capability from USA to Babraham and Cardiff
Year(s) Of Engagement Activity Pre-2006,2006,2007,2008,
 
Description Member of visiting group at University of Athens Biology Department 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Policymakers/politicians
Results and Impact Part of the five-year evaluation of the Biology undergraduate and graduate programme at University of Athens. 4-member panel, one week visit,
Year(s) Of Engagement Activity 2018
 
Description Participated in Babraham Institute exhibit at Royal Society Summer Science Exhibition 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact The Babraham Institute prepared an exhibit - The Ageing Clock - which exemplified aspects of our ageing research portfolio for a public audience. Tjis was selcted tp be part of the prestigious Royal Society Summer Science Exhibition and I was involved in presentign this exhbit to the Public togehter with colleagues.
Year(s) Of Engagement Activity 2018
 
Description Presentation at Instutute Ageing Conference 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Invited talk at Ageing conference, Babraham Institute
Year(s) Of Engagement Activity 2017
 
Description Public feedback exercise 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Professional Practitioners
Results and Impact The Signalling Department, in parallel with other BI departments, presented our research to a professionally selected sample of the UK public with the interntion of gaining public feedback on our institute and research.
Year(s) Of Engagement Activity 2015
 
Description School visits 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact The students were enthused about the topic of my presentation and this led to dialogue and discussion about several issues including new cancer therapies, evolution of drug resistance in cancer, the use of animals in research.


Anecdotally, the institute received requests for summer placement students following this visit.
Year(s) Of Engagement Activity 2014,2015,2016,2017,2018
 
Description Science Open Day 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Schools
Results and Impact Students visited the lab and undertook small lab-based proejcts supervised by students/post-docs and myself. I explained the research that we do and discussed ethical issues such as the use of animals in research.
This precipitated excellent discussion and dialogue.

We received excellent feedback from the schools involved and requests for further outreach activities
Year(s) Of Engagement Activity 2013,2014,2015,2016,2017
 
Description SysMod 2017 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I organised the 2nd meeting of the ISCB Community of Special Interest SysMod. Up to 270 people attended depending on the talk.
Year(s) Of Engagement Activity 2017
 
Description Visits by Teachers 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact I hosted two 6th form Biology teachers (one PhD trained, the other MSc trained) who were visiting my Institution during Half Term to update their knowledge as part of their CPD
Year(s) Of Engagement Activity 2016
 
Description WT/EBI course in silico systems biology 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact I organised this one-week course, and gave presentations entitled: "What is Systems Biology? Where does it come from?", "The many faces of modelling in biology", "Modelling in systems biology, a few challenges", "From art to engineering: two decades of standards and tools towards digital organisms", "Modelling chemical kinetics" plus a tutorial on stochastic simulations of biological systems.
Year(s) Of Engagement Activity 2017
 
Description eLife Community Webinar - Refreshing approaches to researcher evaluation 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I animated a webinar discussing various ways to bypass the traditional publication-based evaluation of researchers.
Year(s) Of Engagement Activity 2017