To study epigenetic mechanisms that create heterogeneity and functional diversity in stem cells, development and between individuals

Lead Research Organisation: Babraham Institute
Department Name: UNLISTED

Abstract

Single-cell technologies have revealed extensive heterogeneity between and within tissues, enabling important new questions in development and ageing to be experimentally addressed. During the course of the last ISPG, for example, we developed scBS-seq23 and the first combined method to sequence the transcriptome and epigenome (methylome) from the same single cell (scM&T-seq24). These new approaches have identified an exciting mechanism by which the creation of epigenetic heterogeneity can be connected with the generation of transcriptional heterogeneity. Building on this work, the new ISPG objective will investigate and model how epigenetic heterogeneity is established and, in turn, leads to diversification of cell phenotype and function during development and ageing. We will test the prediction that as cells transition towards a pluripotent state that is primed for lineage commitment, they acquire epigenetic marks including H3K27me3 and DNA methylation at regulatory regions that act to constrain the expression of developmental TFs25. Rapid turnover of these marks is expected to generate epigenetic heterogeneity between cells and thereby create transcriptional heterogeneity. We propose that this mechanism enables primed pluripotent cells to remain uncommitted, yet able to explore transiently different states that predispose cells toward different lineage choices upon receipt of differentiation stimuli. This informs an important new concept for understanding and controlling lineage commitment during stem cell differentiation and embryo development. In complementary work, using the model organism C. elegans, we have established that there is widespread heterogeneity in a subset of genes that are rate-limiting for ageing. We will, therefore, investigate how epigenetic and transcriptional heterogeneity is established between genetically identical organisms, and how such heterogeneity contributes to variability in key traits during ageing such as lifespan. This Objective uses mouse and human stem cells and C. elegans to explore the emergence and consequences of epigenetic heterogeneity.

Publications

10 25 50
 
Description To investigate the role of Polycomb-group proteins in human pluripotency and molecular heterogeneity (Objective 2.1), we have 1) generated human embryonic stem cells (hESCs) that contain a degron-based PRC2-depletion system to probe the immediate, gene regulatory consequences of Polycomb disruption; 2) we have applied single cell methods to investigate Polycomb protein occupancy and activity in ~200 individual primed-state hESCs, thereby revealing the presence of epigenetic heterogeneity with important consequences for lineage-priming and cell differentiation; 3) mapped the transcriptional and epigenetic changes that occur upon naïve - primed interconversions (PMID 28343983), which have provided new insights into the programming and reprogramming of epigenetic heterogeneity in human cells; 4) completed a genome-wide loss of function screen that has revealed the essential regulators of primed to naïve reprogramming including a switch in Polycomb complex composition that is required for human cells to enter naïve pluripotency; 5) discovered that a Polycomb-mediated network of long-range DNA interactions exists in primed, but not in naïve, hESCs. By developing new network-scale computational approaches, we found that this network connects the promoters and their cis-regulatory regions of many key developmental genes and we hypothesise this contributes to transcriptional co-regulation (preprint, doi: 10.1101/2019.12.13.875286); 6) identified a DNA-binding factor that we believe operates to establish and maintain developmental genes in a 'primed' configuration, thereby providing an exciting molecular mechanism that could underpin the concept of lineage-priming.

We have made several new and exciting discoveries within Objective 2.2. Notably, we have produced a single-cell multi-omics map of gastrulation in mouse embryos, which revealed that regulatory elements associated with each germ layer are either epigenetically primed or remodelled before cell-fate decisions (PMID 31827285). This work uncovers important insights into how the epigenome, operating at multiple molecular layers, controls early cell-fate choice. We have also identified genome-scale oscillations in DNA methylation at the exit of pluripotency particularly in enhancers (PMID 30031774); this is potentially linked to noisy transcription ahead of overt cell fate decision making at gastrulation (PMID 28768204). This could form an important new concept linking molecular heterogeneity with developmental decision-making. We have investigated these ideas in multiple ways: 1) we have identified species-conserved and divergent single cell transcriptomic signatures in mouse, human, and marmoset early development (PMID 30413530); 2) we have compared single cell transcriptional diversity in naïve and primed hESCs (PMID 30673604); 3) we have identified stage- and tissue-specific single cell transcriptional programmes during mouse gastrulation and early organogenesis (PMID 30787436).

We have made good progress towards understanding the causes and consequences of inter-individual variability (Objective 2.3). Specifically, we have established a novel methodology to detect inter-individual variability in gene expression across individual worms. We discovered that stress response genes are variable from individual to individual and the variability arises in specific neurons. Using an in vivo developed marker to amplify the low expression of chaperones under non-stressed conditions, we have established that there is substantial heterogeneity. In parallel, we have found that gene expression heterogeneity also extends to genes that influence fat metabolism and that their variability is inversely correlated with neuronal stress. Using standard epistasis analysis, we have found that forcing chaperone expression in neurons influences fat metabolism genes in a distant tissue, the intestine, using TGF-beta/BMP as a linking signal. We propose that neuronal stress acts as a thermostat to induce desaturation of fats in membranes (preprint, doi: 10.1101/2019.12.20.882514). We propose that this metabolic heterogeneity in an otherwise genetically identical population of individuals improves the adaptation of membranes to warm temperatures.
Exploitation Route Our research is discovering new concepts and developing novel technologies that directly address the role of epigenetic mechanisms that underpin development and ageing, thereby advancing the 'Bioscience for Health' BBSRC priority. We are disseminating our research through scientific publications, presentations at international meetings (e.g. Keystone Single Cell Biology 2019; International Society for Stem Cell Research 2019) and organisation of conferences (e.g. The Ageing Cell, 2017; Metabolism and Epigenetics, 2019), seminar series (Cambridge Epigenetics Club) and workshops (Collaborative Workshops on Epigenetics). In addition, we are training others in using the technologies that we have developed. The Epigenetics ISP have showcased their research at a public exhibit 'Race Against The Ageing Clock' at the Cambridge Science Festival and Royal Society Summer Festival. We are also contributing to the creation of an informed society through taking part in public events about epigenetics, ageing, and gene editing (e.g. at the Cambridge Science Festival; Pint of Science; "Diet and Genes").
Sectors Healthcare,Pharmaceuticals and Medical Biotechnology

 
Description We have organised a successful public event "Diet and Genes: Can we pass on our sins?" together with colleagues at the Cambridge Metabolic Network, which explored the role of epigenetics, diet and metabolism in health and disease. We have also organised a major Royal Society science exhibit "Race Against the Ageing Clock", which involved 42 staff members from our Epigenetics ISPG and an audience of 8400 general educated members of the public. We also seek ways to maximise the impact of our research through collaborations with industry. Completion of work within Objective 2 has led to a research collaboration with Lonza to leverage our understanding of genome organisation to boost the production of therapeutic proteins.
First Year Of Impact 2019
Sector Healthcare,Pharmaceuticals and Medical Biotechnology
Impact Types Societal,Economic

 
Description Chair of SAB Centre for Trophoblast Research University of Cambridge
Geographic Reach National 
Policy Influence Type Participation in a advisory committee
Impact Has changed the perception of placenta and trophoblast in reproduction
 
Description 16ALERT: BD FACS-Aria Fusion - strengthening the cell sorting capabilities of Babraham Institute Flow Core to enrich world-class science
Amount £449,452 (GBP)
Funding ID BB/R00076X/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 08/2017 
End 08/2018
 
Description Enhancer DNA methylation dynamics during early mammalian development
Amount £300,000 (GBP)
Funding ID 215912/Z/19/Z 
Organisation Wellcome Trust 
Sector Charity/Non Profit
Country United Kingdom
Start 08/2019 
End 08/2023
 
Description Flagship
Amount € 1,000,000 (EUR)
Organisation European Commission 
Sector Public
Country European Union (EU)
Start 02/2019 
End 03/2020
 
Description Investigator Award
Amount £2,600,000 (GBP)
Funding ID 210754/Z/18/Z 
Organisation Wellcome Trust 
Sector Charity/Non Profit
Country United Kingdom
Start 10/2018 
End 09/2023
 
Description Special initiative
Amount £10,000,000 (GBP)
Organisation Wellcome Trust 
Sector Charity/Non Profit
Country United Kingdom
Start 05/2019 
End 04/2024
 
Description UKRMP2 Pluripotent Stem and Engineered Cell (PSEC) Hub
Amount £250,000 (GBP)
Organisation Medical Research Council (MRC) 
Sector Public
Country United Kingdom
Start  
 
Description US Partnering Award: Cellular heterogeneity, signalling and decision-making in human pluripotent stem cells
Amount £48,164 (GBP)
Funding ID BB/R021341/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 04/2018 
End 04/2022
 
Title C elegans Metabolic Reconstruction Model 
Description Merged and re-annotated C elegans Metabolic Reconstruction Model 
Type Of Material Technology assay or reagent 
Year Produced 2018 
Provided To Others? Yes  
Impact This is a great improvement to the C elegans research community 
 
Title Identification of cell-surface proteins that can distinguish between human pluripotent states 
Description We have developed a new method to identify and flow-sort human naive and primed pluripotent stem cells based on their different cell-surface protein expression. 
Type Of Material Technology assay or reagent 
Year Produced 2017 
Provided To Others? Yes  
Impact We have published our method (Cell Stem Cell, 2017) and we anticipate other researchers to use the method in their own research. 
 
Title Novel method to study metabolic fluxes during ageing 
Description The novel method has been christened "Metab_FBA" and it is a novel flux balance analysis method to study ageing samples 
Type Of Material Technology assay or reagent 
Year Produced 2019 
Provided To Others? Yes  
Impact This development is a step forward for the field of metabolism and ageing in multicellular model organisms 
 
Title PCR Bayes 
Description Novel algorithm to quantify expression and biological variability of steady state transcripts 
Type Of Material Technology assay or reagent 
Year Produced 2017 
Provided To Others? No  
Impact This is a novel tool which extends the capabilities of C elegans researchers to quantitate biological noise in single worms 
 
Title Parp1/2 knockout 
Description Established mouse knockout lines that lack Parp1 and Parp2. 
Type Of Material Model of mechanisms or symptoms - mammalian in vivo 
Provided To Others? No  
Impact Possibility to study impact of these genes on imprinted gene regulation, the stability of chromatin organisation in general, and DNA damage responses. 
 
Title Single cell multi-omics (scNMT-seq) 
Description Single cell triple-omics which records the transcriptome, methylome, and chromatin accessibility from the same single cell 
Type Of Material Technology assay or reagent 
Year Produced 2018 
Provided To Others? Yes  
Impact This method was applied to the first real biology question in 2019 on an investigation of mouse gastrulation (Argelaguet et al 2019 Nature). The joint first author Stephen Clark was awarded the researcher of the year award 2019 by the Cambridge Independent newspaper. 
 
Title Single cell triple-omics 
Description Single cell triple-omics combining transcriptome, methylome, and chromatin accessibility from the same single cell. 
Type Of Material Technology assay or reagent 
Year Produced 2017 
Provided To Others? Yes  
Impact This method was just published. 
 
Title Tet mutant TSCs 
Description Generated and established trophoblast stem cell lines lacking either Tet1, Tet2 or Tet1+Tet2. 
Type Of Material Cell line 
Year Produced 2017 
Provided To Others? No  
Impact Enabling the assessment of the role of TET enzymes, and their product 5-hydroxymethylcytosine, on trophoblast stem cell maintenance, integrity and differentiation. 
 
Title Worm-Align and Worm_CP 
Description Worm-align/Worm_CP is a simple FIJI/CellProfiler workflow that can be used to straighten and align Caenorhabditis elegans samples and to score whole-worm image-based assays without the need for prior training steps. We have applied Worm-align/Worm_CP to the quantification of heat-shock induced expression in live animals or lipid droplets in fixed samples. 
Type Of Material Biological samples 
Year Produced 2020 
Provided To Others? Yes  
Impact Publication of the method in JOVE journal 
 
Title Aging Cell 2018 
Description We provide expression (RNA-seq) and epigenetic (ChIP-Seq and BS-Seq) data sets for young adult and aged mouse neural stem cells. The data are accessible at GEO under Accession Number GSE101610. 
Type Of Material Database/Collection of data 
Year Produced 2018 
Provided To Others? Yes  
Impact We anticipate that other researchers will use our published data sets to identify candidate regulators of age-associated neurogenic decline. 
 
Title Cell Reports 2018 
Description We have generated transcriptional (RNA-seq), gene regulatory (ChIP-seq) and chromatin organisation (Hi-C and PCHi-C) datasets and deposited them in GEO under accession number GSE103053. 
Type Of Material Database/Collection of data 
Year Produced 2018 
Provided To Others? Yes  
Impact The data sets are published as part of a paper in Cell Reports, and we anticipate that other researchers will use our data for their own work. 
 
Title Cell Stem Cell 2017 
Description We have deposited RNA-sequencing data related to our publication in Cell Stem Cell, 2017. The data have been uploaded to GEO under accession number GSE93241. 
Type Of Material Database/Collection of data 
Year Produced 2017 
Provided To Others? Yes  
Impact The data relate to the reprogramming of human pluripotent stem cells. We anticipate that these data will be used by other researchers. 
 
Title ChIP-seq profiles for Tet1 in TSCs (GSE109545) 
Description Chromatin binding profiles of Tet1 protein in trophoblast stem cells (TSCs) 
Type Of Material Database/Collection of data 
Year Produced 2018 
Provided To Others? Yes  
Impact Data allow to determine the functional role of Tet1 in TSCs 
 
Title Elf5 and Esrrb protein interactomes in TSCs (PXD002183) 
Description IP->MS/MS datasets of interaction partners of the key transcription factors Esrrb and Elf5 in trophoblast stem cells (TSCs). 
Type Of Material Database/Collection of data 
Year Produced 2015 
Provided To Others? No  
Impact Data deposited in publicly accessible databases. Results are instrumental to gain a detailed understanding of how TSCs operate, which will be important to move the field of reproductive biology and pregnancy (normal and pathological) forward. 
URL http://www.ebi.ac.uk/pride/archive/
 
Title Transcriptome datasets of reproductive tissues in ageing females (GSE98901) 
Description RNA-seq datasets of trophoblast, endometrium and deciduae of mouse conceptuses developed in young versus aged females 
Type Of Material Database/Collection of data 
Year Produced 2017 
Provided To Others? Yes  
Impact Significant changes imposed by maternal age on expression profiles of endoemtrial tissue and decidual development. Work shows the impact of the older female on reproductive success, unrelated to oocyte fitness. 
 
Title We submit every year hundreds to thousands of new database entries these are all linked to the publications associated with the grants 
Description please see above 
Type Of Material Database/Collection of data 
Year Produced 2019 
Provided To Others? Yes  
Impact please see above 
 
Title eLife 2017 
Description High-through sequencing data related to our 2017 published in eLife. Data deposited in GEO under accession number GSE86821 
Type Of Material Database/Collection of data 
Year Produced 2017 
Provided To Others? Yes  
Impact We provide the coordinates for promoter-enhancer interactions in human pluripotent cells and differentiated cells. The data will be re-used by other researchers. Too early for measured impacts. 
 
Description Austin Smith 
Organisation University of Cambridge
Country United Kingdom 
Sector Academic/University 
PI Contribution Epigenomics and multi-omics sequencing
Collaborator Contribution Human and mouse pluripotent stem cells
Impact Several collaborative publications.
Start Year 2013
 
Description Aylwyn Scally 
Organisation University of Cambridge
Department Department of Genetics
Country United Kingdom 
Sector Academic/University 
PI Contribution We are collaborating on a research project to investigate the regulation and function of duplicated pseudogenes in human development and pluripotency. My team are contributing expertise, materials and resources related to the gene regulatory control of human pluripotent stem cells.
Collaborator Contribution The Scally team are leaders in evolutionary genetics and have been contributing expertise and computational analyses of pseudogenes in human evolution.
Impact The research collaboration is underway with no reported outputs so far.
Start Year 2019
 
Description Benjamin Simons 
Organisation University of Cambridge
Country United Kingdom 
Sector Academic/University 
PI Contribution Single cell multi-omics datasets.
Collaborator Contribution Modelling methods for multi-omics datasets.
Impact Two collaborative publications, two joint grants. Multi-disciplinary between biology and theoretical physics.
Start Year 2014
 
Description CEGX 
Organisation Cambridge Epigenetix
Country United Kingdom 
Sector Private 
PI Contribution Data generated by CASE PhD student as preliminary/pilot data for target validation of epigenetic changes in disease.
Collaborator Contribution Industrial CASE PhD studentship partner Cambridge Epigenetix (CEGX) provided help and advice in novel, proprietary technique and enabled student to perform this new method of epigenetic target validation during the placement period.
Impact PhD studentship in progress. Manuscript(s) will be generated from this work, possibly even targets for follow-on work and potential commercialisation as epigenetic biomarkers.
Start Year 2016
 
Description COST Action_Worm Genie (COST BM1408) 
Organisation European Union
Country European Union (EU) 
Sector Public 
PI Contribution Financing for traveling and collaborations around europe for other labs in the EU
Collaborator Contribution Financing for traveling and collaborations around europe for my lab members
Impact -knoweldge exchange -conferences -bringing new technologies to the lab -using infrastructure that was not present in house
Start Year 2017
 
Description Claire Rougeulle 
Organisation Paris Diderot University
Country France 
Sector Academic/University 
PI Contribution We have provided cell lines and contributed experiments to an ongoing collaboration.
Collaborator Contribution Our collaborators have made key discoveries about X-chromosome inactivation in human cells.
Impact Manuscript in preparation.
Start Year 2015
 
Description Collaboration with Alvaro Rada-Iglesias 
Organisation University of Cantabria
Country Spain 
Sector Academic/University 
PI Contribution Single cell multi-omics
Collaborator Contribution In vitro PGCLC model
Impact First datasets have been obtained. A training visit has taken place.
Start Year 2019
 
Description Collaboration with Fredrik Lanner 
Organisation Karolinska Institute
Country Sweden 
Sector Academic/University 
PI Contribution Help and assistance with multi-omics single cell sequencing and computational analysis
Collaborator Contribution Collection of human embryo cells
Impact Paper is in preparation. Embryology, single cell genomics, computation.
Start Year 2017
 
Description Collaboration with James Turner 
Organisation Francis Crick Institute
Country United Kingdom 
Sector Academic/University 
PI Contribution We are providing help and training with scNMT-seq
Collaborator Contribution Collected samples from marsupial embryos for sequencing
Impact None yet
Start Year 2018
 
Description Collaboration with Oliver Stegle 
Organisation EMBL European Bioinformatics Institute (EMBL - EBI)
Country United Kingdom 
Sector Academic/University 
PI Contribution We conceived the study and provided sequencing datasets and interpretation.
Collaborator Contribution They helped with conceiving the study and provided computational analysis and modelling.
Impact Initial work has been published in Stubbs et al 2017. The work is multidisciplinary involving epigeneticists and computational biologists and modellers.
Start Year 2016
 
Description Development of computational methods for the quantification of biological variability 
Organisation EMBL European Bioinformatics Institute (EMBL - EBI)
Country United Kingdom 
Sector Academic/University 
PI Contribution Development of an algorithm based on Bayesian statistics that quantifies variability in steady state levels of mRNA from nanofluidics-based PCR.
Collaborator Contribution Development of an algorithm based on Bayesian statistics that quantifies variability in steady state levels of mRNA from nanofluidics-based PCR.
Impact It is a multi-disciplinary collaboration involving high end PCR technology and statistical modelling.
Start Year 2015
 
Description Fredrik Lanner 
Organisation Karolinska Institute
Country Sweden 
Sector Academic/University 
PI Contribution We have made new discoveries about human pluripotent states.
Collaborator Contribution Our collaborators have shared data, provided reagents, and participated in a shared project with us.
Impact None
Start Year 2015
 
Description Gastrulation team 
Organisation University of Cambridge
Country United Kingdom 
Sector Academic/University 
PI Contribution Conceived of the project and lead partner. Contributing single cell multi-omics and embryology.
Collaborator Contribution Helped to conceive of the project and several experimental and computational contributions.
Impact First publication Mohammed et al 2017. Experimental embryology, single cell biology, computation, modelling, epigenetics.
Start Year 2014
 
Description Guiseppe Lupo 
Organisation Sapienza University of Rome
Country Italy 
Sector Academic/University 
PI Contribution We have profiled the epigenome and transcriptome of aged neural stem cells. We have also hosted GL in our laboratory over two summers and provided training in epigenomics techniques.
Collaborator Contribution Our partners derived and characterised young and aged neural stem cells, provide expertise in neural biology, and have given training to us in neural stem cell cultures.
Impact Manuscript in preparation.
Start Year 2014
 
Description Human Cell Atlas 
Organisation Broad Institute
Country United States 
Sector Charity/Non Profit 
PI Contribution Contributed to formulating the white paper of the Human Cell Atlas.
Collaborator Contribution Conceived the project and brought the international consortium together.
Impact The white paper was published Regev et al 2017. It involves single cell biologists and computational and modelling scientists.
Start Year 2016
 
Description Jean-Antoine Girault 
Organisation National Institute of Health and Medical Research (INSERM)
Country France 
Sector Academic/University 
PI Contribution Analysis of data, and predictive modelling
Collaborator Contribution Provision of experimental datasets
Impact We analysed the differences between transcriptomes of D1 and D2 receptor-containing neurons of the basal ganglia, proposed master regulators and unravelled a new signalling system regulating neuronal identity. This is a multi-disciplinary collaboration involving experimental and computational biology.
Start Year 2016
 
Description Jenny Nichols mouse and human embryology 
Organisation University of Cambridge
Country United Kingdom 
Sector Academic/University 
PI Contribution Single cell multi-omics, mutants of epigenetic regulators.
Collaborator Contribution Mouse and human embryos
Impact Several collaborative publications and two joint grants. Multi-disciplinary between epigenetics, multi-omics, embryology, stem cell research.
Start Year 2014
 
Description Jenny Nichols, Austin Smith 
Organisation University of Cambridge
Country United Kingdom 
Sector Academic/University 
PI Contribution Contributed to conceiving studies. Epigenetic profiling and analysis.
Collaborator Contribution Conceived and conducted studies.
Impact Publications Kalkan et al 2017, Guo et al 2017. Involves embryologists, stem cell biologists, computational biologists, epigeneticists.
Start Year 2016
 
Description Kosuke Yusa 
Organisation The Wellcome Trust Sanger Institute
Country United Kingdom 
Sector Charity/Non Profit 
PI Contribution We have together conceived and conducted experiments to identify new regulators of human pluripotency and reprogramming using genome-wide genetic screens.
Collaborator Contribution Our partner provided expertise about the screen design, provided the cell line and lentiviral library, and sequenced the DNA samples.
Impact Manuscript in preparation.
Start Year 2017
 
Description Ludovic Vallier 
Organisation University of Cambridge
Country United Kingdom 
Sector Academic/University 
PI Contribution We are working jointly on investigating the role of TGFb signalling in the control of human pluripotent states.
Collaborator Contribution Our partner provided expertise in TGFb signalling, contributions to experimental design, conducted experiments, and were jointly involved in interpreting results.
Impact The major output so far is the collection of data.
Start Year 2018
 
Description Meri Huch 
Organisation Max Planck Society
Department Center for Systems Biology Dresden
Country Germany 
Sector Charity/Non Profit 
PI Contribution Single cell multi-omics
Collaborator Contribution Mouse model of liver regeneration
Impact Several datasets. Multi-disciplinary between epigenetics, multi-omics, disease modelling.
Start Year 2017
 
Description Mikhail Spivakov 
Organisation Imperial College London
Department MRC London Institute of Medical Sciences
Country United Kingdom 
Sector Academic/University 
PI Contribution We are working together on a research project aimed at understanding the role of poised enhancers in developmental gene regulation. My research team are contributing expertise, resources and materials related to human pluripotent cells, Polycomb-group proteins and developmental priming.
Collaborator Contribution The Spivakov group are leaders in the field of 3D genome organisation and transcriptional control, and they are providing expertise, resources and materials related to these topics.
Impact The project is underway with no outputs to date.
Start Year 2019
 
Description Sanjay Sinha 
Organisation Wellcome Trust
Department Wellcome - MRC Cambridge Stem Cell Institute
Country United Kingdom 
Sector Academic/University 
PI Contribution Analysis of single cell transcriptomes.
Collaborator Contribution Provision of datasets.
Impact We unravelled the cellular heterogeneity of cultures of engineered epicardium, characterised two populations with different differentiation potential, found a mean to enrich the culture with one population, and proposed a chemical cell reprogramming method. This is a multi-disciplinary collaboration involving experimental and computational biology.
Start Year 2014
 
Description Sean Bendall 
Organisation Stanford University
Country United States 
Sector Academic/University 
PI Contribution We are working together to devise new ways to measure and understand heterogeneity in human pluripotent cells, including being able to associate protein, epigenetic and signalling variability in the same individual cells.
Collaborator Contribution Our partner is a leader in the field of mass cytometry and is contributing to the experiment design and execution, including data analysis.
Impact This collaboration led to additional funding through the BBSRC UK - USA Partnership Award.
Start Year 2017
 
Description Steffen Rulands 
Organisation Max Planck Society
Department Max Planck Institute for the Physics of Complex Systems
Country Germany 
Sector Academic/University 
PI Contribution Single cell multi-omics datasets
Collaborator Contribution Modelling approaches for single cell multi-omics datasets
Impact One collaborative publication. Multi-disciplinary between biology and theoretical physics.
Start Year 2015
 
Description Thorsten Boroviak - single cell multi-omics of primate development 
Organisation University of Cambridge
Country United Kingdom 
Sector Academic/University 
PI Contribution Carrying out single cell multi-omics on samples from non-human primates
Collaborator Contribution Providing samples from non-human primates
Impact See publications. Developmental biology, epigenomics, computational biology.
Start Year 2017
 
Description single cell TSCs 
Organisation University of Cambridge
Department Department of Obstetrics & Gynaecology
Country United Kingdom 
Sector Academic/University 
PI Contribution Collaboration with Prof Steve Charnock Jones to form core of Centre of Trophoblast Research single cell transcriptomics profiles of mouse (and human) trophoblast stem cells under various conditions, as well as of placentas. Provided cells and tissues and culture media components to collaborator.
Collaborator Contribution Set up Drop-seq and optimising technique for nuclear RNA sequencing, required for syncytia. Performed sc-RNA-seq.
Impact Manuscript in preparation.
Start Year 2017
 
Description uNK cells 
Organisation University of Cambridge
Country United Kingdom 
Sector Academic/University 
PI Contribution Characterisaiton of reproductive problems in older females.
Collaborator Contribution Analysis of immune repertoire of implantation sites depending on maternal age.
Impact Collaborative publication (PMID: 28874785).
Start Year 2016
 
Title CASE studentship agreement 
Description A studentship in collaboration with industry 
IP Reference  
Protection Protection not required
Year Protection Granted 2015
Licensed No
Impact The student has successfully passed their viva
 
Title Collaboration agreement 
Description This collaboration agreement covers work to be carried out under the Wellcome Human Developmental Biology Initiative 
IP Reference  
Protection Protection not required
Year Protection Granted 2019
Licensed No
Impact None yet
 
Title Exclusive license agreement 
Description Antibodies against DNA modifications 
IP Reference  
Protection Protection not required
Year Protection Granted 2016
Licensed No
Impact Income to the Institute
 
Title Methylome of human blastocysts 
Description Collaboration agreement with Karolinska Institute 
IP Reference  
Protection Protection not required
Year Protection Granted 2017
Licensed No
Impact Several datasets have been obtained
 
Title Non-exclusive license agreement 
Description Antibodies against DNA modifications 
IP Reference  
Protection Protection not required
Year Protection Granted 2016
Licensed Commercial In Confidence
Impact Income to the Institute
 
Title Non-exclusive license agreement 
Description Antibodies against DNA modifications 
IP Reference  
Protection Protection not required
Year Protection Granted 2011
Licensed Commercial In Confidence
Impact Income to the Institute
 
Description 15th International Curie Course on Epigenetics - Paris 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Invited talk
Year(s) Of Engagement Activity 2019
 
Description 2019 Keystone Symposia on Single Cell Biology - Colorado 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Invited talk
Year(s) Of Engagement Activity 2019
 
Description 3rd Revolutionizing Next-Generation Sequencing Conference - Belgium 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Invited talk
Year(s) Of Engagement Activity 2019
 
Description A talk at "Networks of Cellular Surveillance Mechanisms" Conference 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact An invited talk at an Interational Conference in Heidelberg
Year(s) Of Engagement Activity 2018
 
Description A talk at the Longevity leaders workshop in Oxford, UK 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Professional Practitioners
Results and Impact I gave an invited talk at this foru organised by the companu Juvenescence
Year(s) Of Engagement Activity 2019
 
Description An invited talk at "Advances in metabolic communication" conference 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact The conference was organised by Nature Metabolism in Brazil
Year(s) Of Engagement Activity 2019
 
Description An invited talk at conference "Networks Of Cellular Surveillance Mechanisms" 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact International conference in Heidelberg, Germany
Year(s) Of Engagement Activity 2018
 
Description Babraham Schools day 2018 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Experiments on epigenetics for local 6th form schools
Year(s) Of Engagement Activity 2018
 
Description Bioinformatic Information Evening 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Explaining the use of bioinformatics within our research to a group of 20 teachers from the local area. Primarily to teacher and secondarily the passing on of information to the students they teach.
Year(s) Of Engagement Activity 2018
 
Description Biology of Aging Gordon Research Conference - Maine, USA 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Invited talk
Year(s) Of Engagement Activity 2019
 
Description Cambridge Epigenetics Club 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Professional Practitioners
Results and Impact 150 people attended an evening of scientific presentations and networking as part of the Cambridge Epigenetics Club. This happens ~6 times per year. I organise these events with colleagues at the University of Cambridge, and the Club is sponsored by Abcam. The main outcomes are to bring together people from academia, industry, schools, and the general public to interact and discuss cutting edge epigenetics research.
Year(s) Of Engagement Activity 2018,2019,2020
URL https://www.abcam.com/events/cambridge-epigenetics-club
 
Description Cambridge Science Festival 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Three members of my team participated in the Cambridge Science Festival, presenting their research using the 'Race Against the Ageing Clock' exhibit.
Year(s) Of Engagement Activity 2019
 
Description Cambridge Science Festival 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Media (as a channel to the public)
Results and Impact This was a presentation for wider audience by laetitia Chauve
Year(s) Of Engagement Activity 2019
 
Description Cambridge Science Festival 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Babraham Institute exhibit on the Epigenetic Ageing Clock presented at the Cambridge Science Festival, involving activities and question/answer interactions with members of the public
Year(s) Of Engagement Activity 2018
 
Description Co-organised an international conference on "Advances at the interface between metabolism and epigenetics" 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I helped to organise an international conference on the theme of epigenetics and metabolism. This brought together scientists from different disciplines and sparked new interests and collaborations.
Year(s) Of Engagement Activity 2019
 
Description Co-organised public event "Diet and Genes: Can we pass on our sins?" 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact I helped to organise a public event that discussed topics related to epigenetics, diet, metabolism. This generated a lively discussion and lots of interest for research in this area.
Year(s) Of Engagement Activity 2019
 
Description Collaborative Workshop on Epigenetics 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact I co-organised a cross-institute workshop on epigenetics, which sparked new interactions and potential collaborations between researchers at different BBSRC-supported institutes.
Year(s) Of Engagement Activity 2020
 
Description Computational Modeling in Biology Network 
Form Of Engagement Activity Engagement focused website, blog or social media channel
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I created COMBINE to coordinate the development of standards in computational systems biology. The initiative includes several workshops a year (discussion of future standards, implementation of current standards, and end-user training), diffusion lists, social media dissemination.
Year(s) Of Engagement Activity 2011,2012,2013,2014,2015,2016,2017,2018
URL http://co.mbine.org
 
Description Cross-Institute Workshop on Epigenetics 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact I co-organised a cross-institute workshop on epigenetics, which sparked new interactions and potential collaborations between researchers at different BBSRC-supported institutes.
Year(s) Of Engagement Activity 2018
 
Description DNA extraction from fruit Babraham Campus visit from Home educated children 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact Extract DNA from fruit whilst showing how to "be a scientist" to home schooled children.
Impact: hands on activity to explain the basics of DNA and promote a career in research to home schooled children.
Year(s) Of Engagement Activity 2016
 
Description Distinguished Lecture Speaker - University of Pennsylvania 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact Invited talk
Year(s) Of Engagement Activity 2019
 
Description EMBL Conference: Chromatin and Epigenetics - Heidelberg 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Invited talk
Year(s) Of Engagement Activity 2019
 
Description Epigenetics - Making Your Mark 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Schools
Results and Impact We developed materials for sixth-form students and teachers, including classroom activities and experiments to try at home. The materials are available online (URL below) and have been presented at the following events:

Oct 2017:
Hills Road Sixth Form College Big Biology Day to an estimated audience of 500 members of the general public

June 2018:
UKRI Launch event in Swindon to an estimated audience of 75 adults (UKRI/BBSCR staff)

July 2018:
The 8th Euroscience Open Forum Conference in Toulouse to an estimated audience of 50 adults
Year(s) Of Engagement Activity 2017,2018
URL https://www.babraham.ac.uk/about-us/impact/public/resources/sixth-form-colleges
 
Description GENIE network of C elegans researchers 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Joining this pan european network has allowed us to attend conferences, network with other C elegans labs across Europe and obtaining funding to organise a workshop on C elegans metabolic reconstruction.
Year(s) Of Engagement Activity 2015,2016,2017,2018
 
Description GENiE workshop "Extending the consensus representation of C. elegans metabolism" 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Study participants or study members
Results and Impact https://www.helmholtz-muenchen.de/genie-workshop-2017/program/index.html
Year(s) Of Engagement Activity 2018
URL https://www.helmholtz-muenchen.de/genie-workshop-2017/program/index.html
 
Description Gene editing (Cambridge) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact 120 people attended a panel discussion about gene editing, with lots of questions from the audience and discussion afterwards, and there were several follow-up conversations from the media and patient groups.
Year(s) Of Engagement Activity 2016
 
Description General public workshop "Cambridge Climate and sustainability festival" 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact Invited speaker at "Cambridge Climate and sustainability festival" at St John's college February 16th, 2019
Year(s) Of Engagement Activity 2019
 
Description Genetics Society and BSDB Spring Meeting, Warwick, UK (MH) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Invited speaker at annual spring meeting of the Genetics Society and British Society for Developmental Biology (BSDB) in Warwick, UK (2017)
Year(s) Of Engagement Activity 2017
 
Description Genetics Society annual autumn meeting - poster presentation RS 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Presentation of a poster with the newest data on this work. Meeting held in London, UK (2017).
Year(s) Of Engagement Activity 2017
 
Description Genome editing workshop 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Workshop about Crispr genome editing at the Cambridge Science Festival
Year(s) Of Engagement Activity 2019
 
Description Hosted teachers 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact We hosted two teachers in our laboratory, which sparked questions and discussion about our research area.
Year(s) Of Engagement Activity 2018
 
Description IMP Research Institute of Molecular Pathology - Vienna 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact Invited talk
Year(s) Of Engagement Activity 2019
 
Description Institute for Continuing Learning 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Laetitia Chauve participated in a program designed to show Epigenetics in live nematodes
Year(s) Of Engagement Activity 2019
 
Description Invitation to give lecture at the Annual Meeting of the Portuguese Society of Human Genetics 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Gave invited talk at the Annual Meeting of the Portuguese Society of Human Genetics, which sparked questions and interest in our work from academics and industry.
Year(s) Of Engagement Activity 2018
 
Description Invited lecture at Stanford University 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I gave an invited lecture at Stanford University.
Year(s) Of Engagement Activity 2018
 
Description Invited lecture at the European Society of Human Genetics congress 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I gave an invited lecture at the European Society of Human Genetics congress, which led to discussions and new collaborations.
Year(s) Of Engagement Activity 2018
 
Description Invited lecture at the MRC Centre for Regenerative Medicine, Edinburgh 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact I gave an invited lecture that was attended by ~120 people.
Year(s) Of Engagement Activity 2019
 
Description Invited lecture at the University of Vienna 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I gave an invited lecture at the University of Vienna.
Year(s) Of Engagement Activity 2018
 
Description Invited scientific lecture to the Executive Chair and other senior staff of the BBSRC 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact I gave an invited scientific lecture to the Executive Chair and other senior staff of the BBSRC.
Year(s) Of Engagement Activity 2018
 
Description Invited speaker in a conference:"Advances at the interface between metabolism and epigenetics" 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Invited speaker in the conference: "Advances at the interface between metabolism and epigenetics", held in Cambridge, UK
Year(s) Of Engagement Activity 2018
 
Description Invited speaker invitation - Grand Rapids, USA 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact Invited talk
Year(s) Of Engagement Activity 2019
 
Description Invited speaker to a conference: "Systems biology of model organisms" 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Invited speaker at workshop "Systems biology of model organisms" held in Bordeaux, France.
Year(s) Of Engagement Activity 2019
 
Description Invited talk at Edinburgh Chromatin Club 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Invited presentation at the Edinburgh Chromatin Club.
Year(s) Of Engagement Activity 2019
 
Description Invited talk at Presdales School 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Schools
Results and Impact I gave an invited talk about our research to sixth form students at Presdales School. This generated lots of questions and discussion.
Year(s) Of Engagement Activity 2018
 
Description Invited talk at StemCell Technologies UK HQ 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Industry/Business
Results and Impact I gave an invited talk at StemCell Technologies about our research, which generated lots of questions and discussion.
Year(s) Of Engagement Activity 2018
 
Description Invited talk at the International Society for Stem Cell Research 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Invited presentation at the International Society for Stem Cell Research Annual Meeting
Year(s) Of Engagement Activity 2019
 
Description Keynote speaker, CTR Anniversary Meeting, Cambridge, UK (MH) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact Keynote lecture at 10th Anniversary Meeting of the Centre for Trophoblast Research, University of Cambridge (2017)
Year(s) Of Engagement Activity 2017
 
Description LaunchPad Activity Day 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Schools
Results and Impact In April, 12 Year 8 students from The Stephen Perse Foundation and Swavesey Village College visited the Babraham Institute to learn more about laboratory science in a real-world context. Two PhD researchers from my team led students through activities that allowed them to explore how techniques introduced at school were used in a real-life application. The Cambridge LaunchPad programme, which is managed by Form the Future CIC, aims to educate and enthuse young people with STEM subjects.
Year(s) Of Engagement Activity 2019
 
Description LaunchPad filming for secondary schools 
Form Of Engagement Activity A broadcast e.g. TV/radio/film/podcast (other than news/press)
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Schools
Results and Impact Working with a professional media company, two PhD researchers in my team contributed to a short film called "Discovering DNA", as part of the wider LaunchPad activity.
Year(s) Of Engagement Activity 2019
URL https://www.ohfilms.com/portfolio/babraham-institute/
 
Description Launchpad School's Project 
Form Of Engagement Activity Engagement focused website, blog or social media channel
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Schools
Results and Impact The activity involved explaining the research conducted at the Babraham Institute and setting a challenge to the children to optimise the extraction of DNA from strawberries. The challenge involved the design of a poster and was used as a test of teamwork, communication and motivation, with the winning teams being invited to the Institute to learn more about the work we do. The overall aim of this engagement was to inspire the next generation of research scientists in the Cambridge area.
Year(s) Of Engagement Activity 2019
 
Description Lecture to A-level students on Epigenetics 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Lecture on Epigenetics to around 100 A-level students.
Year(s) Of Engagement Activity 2018
 
Description Lecture to medical students on Epigenetics 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Undergraduate students
Results and Impact Lecture on Epigenetics to medical students for the Universidad Autónoma de Chihuahua, Mexico.
Year(s) Of Engagement Activity 2017
 
Description Life Science Symposium 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Postgraduate students
Results and Impact A symposium bringing together over 100 Cambridge University life science students to promote interaction and knowledge exchange between biological fields.
Year(s) Of Engagement Activity 2019
 
Description Life2019 Conference - Netherlands 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Invited talk
Year(s) Of Engagement Activity 2019
 
Description Meeting organiser, Reproduction and Development, Cambridge 2018 (MH) 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact Co-organisation of meeting, Reproduction and Development, held in Cambridge, UK (2018).
Year(s) Of Engagement Activity 2018
 
Description Member of BBSRC Regenerative Biology Working Group 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Invited membership of the BBSRC working group on Regenerative Biology.
Year(s) Of Engagement Activity 2018,2019
 
Description Nine invited presentations at international meetings, including EMBO, FASEB, Keystone, American Association for Cancer Research 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Conference presentations about our research
Year(s) Of Engagement Activity 2018
 
Description ORION Public Dialogue Workshop 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact As part of the Europe-wide ORION Open Science project, we participated in a public dialogue on genome editing, bringing together scientists, ethicists and the public to discuss their hopes and fears for the future of genome editing.
Year(s) Of Engagement Activity 2019
 
Description ORION Stakeholder Workshop 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Study participants or study members
Results and Impact We participated in a stakeholder workshop to help frame materials for a public dialogue on genome editing later in 2019. Participants discussed issues surrounding the future of genome editing, including technical, social, ethical and political aspects of genome editing technologies.
Year(s) Of Engagement Activity 2019
 
Description Organisation of C.elegans Cambridge local area meetings 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact An audience of about 10-15 science labs from around the Cambridge area attend these events, including many PhD students and postdocs. The aim of these meetings is dissemination of techniques and science as well as networking.
Year(s) Of Engagement Activity 2015,2017,2018
 
Description Organisation of an International Conference 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact TAGC Allied Genetics conference in Washington, DC, USA.
Year(s) Of Engagement Activity 2020
 
Description Organisation of an International Conference 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact European C.elegans Conference, Marseille, France.
Year(s) Of Engagement Activity 2020
 
Description Organisation on International Conference 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Organisation of european C.elegans conference
Year(s) Of Engagement Activity 2018
 
Description PGCE Teacher Day 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact We hosted secondary school teachers for the day in our laboratory, providing them with insight into the research process and current topics in epigenetics.
Year(s) Of Engagement Activity 2019
 
Description Partcipation in The Future of Ageing 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact A representative of the Casanueva lab attended a debate on the future of gein at London School of Economics geared towards general public.
Year(s) Of Engagement Activity 2018
 
Description Pilot Collaborative Ethnography with Bioscientists at BI Engaged in Modelling Ageing 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Policymakers/politicians
Results and Impact Working Paper Presentations & Articles in Process:
Based on this initial research, Joanna and Carrie have an article in preparation for Medical Anthropology Quarterly. The article is entitled: "Working with models of ageing: Entanglements of human and animal health and wellbeing." This paper was presented at the Life Sciences & Society Lab, Centre of Sociological Research (CeSO), KU Leuven, Parkstraat 45, 3000 Leuven, Belgium 24th- 25th October.
In addition, Carrie will draw upon her research in writing one chapter of her book on Care as Science, which she plans to start writing in 2018.
Joanna is also drawing on her research at BI in two further papers.
The first, a methodological exploration, draws together the pilot study work at BI with her earlier work on the NDA project with David Kipling: Latimer, J. (forthcoming) Knowledge, life & intimacy: being alongside the life sciences of ageing. For publication in: Latimer, J. & Lopez, D. (eds.) Intimate Entanglements. The Sociological Review Special Issue/Monograph Series. London: Sage. This paper has been presented at Disrupting the Human. Science in Public Annual Conference, iHuman, Sheffield Universiy, 13-15th July, and at a recent workshop Intimate Entanglements, York Medical Society, 19-20 February 2018.
The second draws together the work at BI with Gould's PhD study (funded by the ESRC) An ethnographic study of anti-ageing science: Gould, S. and Latimer, J. Giving Life Time: Reimagined Futures and Interrupted Becoming/s at the Frontiers of the Life Science of Ageing. For submission to Ageing & Society. This paper was presented at the Anthropological Association Gerontology Group Annual Conference. Oxford Brookes University, June, 2017.
Year(s) Of Engagement Activity 2017,2018
 
Description Pint of Science 2018, Cambridge 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact I gave an invited talk at the 2018 Pint of Science Festival in Cambridge, about epigenetics and human development. This sparked questions and discussion.
Year(s) Of Engagement Activity 2018
 
Description Podcast with Dr. Kat Arney about stem cells and epigenetics 
Form Of Engagement Activity A broadcast e.g. TV/radio/film/podcast (other than news/press)
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact Interview with Dr. Kat Arney (Naked Scientists podcast) about stem cells and epigenetics.
Year(s) Of Engagement Activity 2018
 
Description Postgraduate course Epigenetics in Reproductive Biology, University of Murcia, Spain (VPG) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Postgraduate students
Results and Impact Invited talk and workshop participation as part of postgraduate course on reproductive epigenetics
Year(s) Of Engagement Activity 2016,2017
 
Description Royal Society Summer Exhibition 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Babraham Institute exhibit on the Epigenetic Ageing Clock as invited entry presented at the Royal Society Summer Exhibition, involving activities and question/answer interactions with members of the public, fellows of the Royal Society, policymakers
Year(s) Of Engagement Activity 2018
 
Description Savile Club epigenetics talk 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact Presentation about epigenetics at the London Savile Club
Year(s) Of Engagement Activity 2018
 
Description Schools Day 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact We invited students to our lab to show them C elegans
Year(s) Of Engagement Activity 2018,2019,2020
 
Description Schools' Day 2018 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Schools
Results and Impact 120 pupils attended for a school visit to the research organisation, which involved some of the pupils spending time in our lab, and the school reported increased interest in stem cells and epigenetics.
Year(s) Of Engagement Activity 2018
 
Description Schools' Day 2019 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Schools
Results and Impact 75 pupils attended for a school visit to Babraham, which sparked questions and discussions afterwards, and the schools reported increased interest in related subject areas.
Year(s) Of Engagement Activity 2019
 
Description Schools' Day 2019 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Schools
Results and Impact My group hosted eight school students throughout the day and we worked on experiments related to epigenetics and stem cell research.
Year(s) Of Engagement Activity 2019
 
Description Schools' Day 2020 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Schools
Results and Impact My group hosted seven GCSE-stage school students throughout the day and we worked on experiments related to epigenetics and stem cell research.
Year(s) Of Engagement Activity 2020
 
Description Science festival event - CHROMOS 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact Exploration of chromatin data through virtual reality, and presentation of CHROMOS music and video, a multidimensional art piece on genome structure.
Year(s) Of Engagement Activity 2017
URL https://www.babraham.ac.uk/news/2017/02/chromos
 
Description Sophianum School collaboration - Technasium 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Schools
Results and Impact The Babraham Institute is continuing to coordinate activities supporting its commitment as a signatory of the Concordat on Openness on Animal Research. Institute staff, including animal unit managers, have been working closely with a school in the Netherlands to prepare and launch the Institute's contribution to the national 'Technasium' education programme, which aims to draw together students and industry.

Two projects, developed in collaboration with the Institute's Public Engagement team and teachers from the Sophianum School in Gulpen, will challenge students to research and design systems to improve the efficiency and safety of animal care systems. A third project will require the students to research how legislation regulating the use of animals in science, as well as public opinion, can vary throughout Europe and the world.

A two-day visit on 19th & 20th May will kick-start a 10-week programme for the 15-year old students, who will work in teams and compete to design the best solutions to the challenges. The Institute's animal technicians will monitor student progress by commenting on YouTube videos posted fortnightly by the teams. Regular video calls into the animal unit and the Institute's laboratories will allow live discussion between the students, the animal technicians and the scientists who use animals as part of their research.

The challenge projects will finish in July, with the winning Sophianum team coming to the Babraham Institute as part of their prize. The students will visit Institute animal facilities and will get a chance to see the award-winning camera system which displays live feeds from the facility - allowing groups to appreciate first-hand both the animal care environment and the nature of working within an animal research unit.

This pilot project will also involve sixth-form students at the school who will learn about the Institute's research and take part in workshops to discuss the ethics of the use of animals in science. The Institute has been running ethics workshops for secondary and sixth form students since 2005, with contributions from Understanding Animal Research, the European Animal Research Association and the Institute of Animal Technology.

The success of the Technasium project will be evaluated by both the Institute and Sophianum School, with a view to developing future challenges.
Year(s) Of Engagement Activity 2017,2018
 
Description Sophianum poster workshop 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Schools
Results and Impact A scientific poster workshop for the Sophianum school in the Netherlands
Year(s) Of Engagement Activity 2019
 
Description Sophianum sixth form project judging 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Schools
Results and Impact Sixth form students wrote scientific projects which were then judged
Year(s) Of Engagement Activity 2019
 
Description Sophianum visit to BI 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Schools
Results and Impact School visit by dutch school classes
Year(s) Of Engagement Activity 2019
 
Description St Catharines Society Visit 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact A presentation and discussion at the St Catherine's society
Year(s) Of Engagement Activity 2019
 
Description SysMod 2017 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I organised the 2nd meeting of the ISCB Community of Special Interest SysMod. Up to 270 people attended depending on the talk.
Year(s) Of Engagement Activity 2017
 
Description Talk at Cambourne Library 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact 15 general audience attended an Epigenetics presentation and discussion
Year(s) Of Engagement Activity 2018
 
Description Talk for Pint of Science 2017 - Cambridge 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact Pint of Science talk about Epigenetics to an audience of about 50 adults.
Year(s) Of Engagement Activity 2017
 
Description Teachers Day 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Schools
Results and Impact My team hosted several teachers in our laboratory, providing the teachers with insights into research processes and current topics in epigenetics.
Year(s) Of Engagement Activity 2019
 
Description The Ageing Cell Conference, Cambridge, UK (MH) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact Invited talk at The Ageing Cell Conference held in Cambridge, UK (2017)
Year(s) Of Engagement Activity 2017
 
Description Twilight teacher training event, Bioscience lite-CRISPR-Cas9 genome editing- technology, applications and implication, Cambridge (UK) (VPG) 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Schools
Results and Impact Talk and Q&A session with high school teachers to update and inform them on state-of-the-art genome editing tools
Year(s) Of Engagement Activity 2017
 
Description UK - Japan Bilateral International Meeting 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I participated in, and gave an invited lecture, at the UK - Japan Bilateral International Meeting, organised by the Royal Society and the Japan Society for the Promotion of Science (JSPS). The meeting led to increased interest in our research, and new connections with researchers from Japan.
Year(s) Of Engagement Activity 2018
 
Description UKRI launch 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Policymakers/politicians
Results and Impact UKRI launch event 2018
Year(s) Of Engagement Activity 2018
 
Description WT/EBI course in silico systems biology 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact I organised this one-week course, and gave presentations entitled: "What is Systems Biology? Where does it come from?", "The many faces of modelling in biology", "Modelling in systems biology, a few challenges", "From art to engineering: two decades of standards and tools towards digital organisms", "Modelling chemical kinetics" plus a tutorial on stochastic simulations of biological systems.
Year(s) Of Engagement Activity 2017
 
Description Weert College ethics workshop 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Schools
Results and Impact This was a scientific ethics workshop for a school in the Netherlands
Year(s) Of Engagement Activity 2019
 
Description Weert College presentation judging 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Schools
Results and Impact Students were making scientific presentations which were then judged
Year(s) Of Engagement Activity 2019
 
Description Wellcome Genome Campus Cell Fate Workshop 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact This was an invitation only workshop organised by us on the Hinxton campus which brought together some of the international leaders working on cell fate biology and pathology.
A meeting report was produced subsequently.
Year(s) Of Engagement Activity 2018
 
Description Wellcome Trust Workshop on NEURONAL MATURATION: London 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Invited talk
Year(s) Of Engagement Activity 2019
 
Description Whittlewomen 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact Presentation to local women's group
Year(s) Of Engagement Activity 2019
 
Description Women's Institute visit 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Visit by members of the Women's Institute for a discussion and workshop on Epigenetics
Year(s) Of Engagement Activity 2019
 
Description eLife Community Webinar - Refreshing approaches to researcher evaluation 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I animated a webinar discussing various ways to bypass the traditional publication-based evaluation of researchers.
Year(s) Of Engagement Activity 2017