Lipid-derived signalling
Lead Research Organisation:
Rothamsted Research
Department Name: UNLISTED
Abstract
Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.
Technical Summary
Objectives:
1. Defining the role of genes induced by the volatile cis-jasmone (cJ). Transgenic arabidopsis plants will be generated in which cJ-induced genes are ectopically expressed. These lines will be subject to biochemical and metabolomic characterisation, and will also be used in plant-insect studies
2. Defining the signalling pathway utilised by cis-jasmone to effect changes in Arabidopsis gene expression. Candidate transcription factors (such as WRKY, ORCA, MYB and TGA classes) will be evalutated for their contribution to this signalling pathway via reverse genetics. Natural variation between Arabidopsis ecotypes will also be used to identify loci involved in responding to this signal.
3. Defining the role of sphingolipids and sphingolipid long chain bases in plants. Arabidopsis mutants defective in the synthesis of specific sphingolipids will be isolated and characterised at the biochemical and cellular level. Specifically, the role of sphingosine and S-1-P will be defined in plants.
1. Defining the role of genes induced by the volatile cis-jasmone (cJ). Transgenic arabidopsis plants will be generated in which cJ-induced genes are ectopically expressed. These lines will be subject to biochemical and metabolomic characterisation, and will also be used in plant-insect studies
2. Defining the signalling pathway utilised by cis-jasmone to effect changes in Arabidopsis gene expression. Candidate transcription factors (such as WRKY, ORCA, MYB and TGA classes) will be evalutated for their contribution to this signalling pathway via reverse genetics. Natural variation between Arabidopsis ecotypes will also be used to identify loci involved in responding to this signal.
3. Defining the role of sphingolipids and sphingolipid long chain bases in plants. Arabidopsis mutants defective in the synthesis of specific sphingolipids will be isolated and characterised at the biochemical and cellular level. Specifically, the role of sphingosine and S-1-P will be defined in plants.
Planned Impact
unavailable
Organisations
People |
ORCID iD |
| Johnathan Napier (Principal Investigator) |
Publications
Capuano F
(2011)
LC-MS/MS Methods for Absolute Quantification and Identification of Proteins Associated with Chimeric Plant Oil Bodies
in Analytical Chemistry
Matthes M
(2011)
Emerging roles in plant defense for cis-jasmone-induced cytochrome P450 CYP81D11.
in Plant signaling & behavior
Matthes MC
(2008)
Natural variation in responsiveness of Arabidopsis thaliana to methyl jasmonate is developmentally regulated.
in Planta
Matthes MC
(2010)
The transcriptome of cis-jasmone-induced resistance in Arabidopsis thaliana and its role in indirect defence.
in Planta
Michaelson LV
(2009)
Functional characterization of a higher plant sphingolipid Delta4-desaturase: defining the role of sphingosine and sphingosine-1-phosphate in Arabidopsis.
in Plant physiology
Michaelson LV
(2010)
Viral trans-dominant manipulation of algal sphingolipids.
in Trends in plant science
Nissimov JI
(2012)
Draft genome sequence of the coccolithovirus Emiliania huxleyi virus 202.
in Journal of virology
Nissimov JI
(2011)
Draft genome sequence of the Coccolithovirus Emiliania huxleyi virus 203.
in Journal of virology
Nissimov JI
(2011)
Draft genome sequence of the coccolithovirus EhV-84.
in Standards in genomic sciences
Reina-Pinto JJ
(2009)
Lipid determinants of cell death.
in Plant signaling & behavior
| Description | The importance of lipid (volatile) signals in how plants and other simple organisms communicate |
| Exploitation Route | . |
| Sectors | Agriculture Food and Drink |