TPM - Tailoring Plant Metabolism - Work package 1 (WP1) - High value lipids for health and industry
Lead Research Organisation:
Rothamsted Research
Department Name: Unlisted
Abstract
Plant seeds are a reservoir of energy-dense molecules, including oils enriched in triacylglycerols (TAGs) that serve as the primary storage form of fatty acids. TAGs and their component fatty acids not only provide a major dietary calorie source but also serve as a renewable source of hydrocarbons for biofuels and industrial applications. By understanding and exploiting the diversity of lipid biosynthetic pathways in nature, seeds can be used as a canvas for the design and tailoring of biochemical pathways to generate diverse nutritional and industrial oils not found in oilseed crops. WP1 will examine the spatiotemporal regulation of lipid synthesis, using a combination of transcriptomics, lipidomics, mass spec imaging, tracer studies and metabolic modelling. The interactions between endogenous metabolism and introduced biochemical pathways will be analysed using Camelina lines engineered to produce non-native, very long chain polyunsaturated fatty acids. The potential to exploit novel regulatory mechanisms (including nuclear RNA export and targeted protein degradation) will also be explored. Finally, genome editing and conventional genetic improvement will be employed to improve the Camelina germplasm used for engineering (the "chassis"). Together, these approaches will provide a knowledge base to facilitate predictive metabolic engineering of a range of high-value lipids, including polyunsaturated fatty acids with proven health and nutritional benefits, wax esters for the lubricant and cosmetics markets and structured TAGs with desirable properties.
Organisations
- Rothamsted Research, United Kingdom (Lead Research Organisation)
- International Rice Research Institute, Philippines (Collaboration)
- University of Southampton, United Kingdom (Collaboration)
- University College London, United Kingdom (Collaboration)
- National Physical Laboratory NPL, United Kingdom (Collaboration)
- Ingenza Ltd, United Kingdom (Collaboration)
- University of Bath, Bath (Collaboration)
- Punjab Aricultural University (Collaboration)
- Cargill Europe (Collaboration)
- University of Liverpool, United Kingdom (Collaboration)
- University of Cambridge (Collaboration)
- Cardiff University, United Kingdom (Collaboration)
- Unilever R&D Vlaardingen B.V., Netherlands (Collaboration)
- Commonwealth Scientific and Industrial Research Organisation (Collaboration)
- University of Heidelberg, Germany (Collaboration)
- University of Nottingham (Collaboration)
- National Inst of Agricultural Botany (Collaboration)
- French National Institute of Agricultural Research (Collaboration)
- Protein Technologies Ltd, Cheadle, United Kingdom (Collaboration)
- University of York, United Kingdom (Collaboration)
- University of Huelva (Collaboration)
- Universidade de São Paulo (Collaboration)
- Pilot Pflanzenöltechnologie Magdeburg (Collaboration)
- Cargill (Collaboration)
- University of Buenos Aires, Argentina (Collaboration)
- University of Nebraska-Lincoln, United States (Collaboration)
- Swedish University of Agricultural Sci, Sweden (Collaboration)
- University of Stockholm (Collaboration)
- Instituto de la Grasa (Collaboration)
Publications
Amiruddin N
(2020)
Characterization of Oil Palm Acyl-CoA-Binding Proteins and Correlation of Their Gene Expression with Oil Synthesis.
in Plant & cell physiology
Barrero-Sicilia C
(2017)
Lipid remodelling: Unravelling the response to cold stress in Arabidopsis and its extremophile relative Eutrema salsugineum.
in Plant science : an international journal of experimental plant biology
Betancor MB
(2021)
Oil from transgenic as a source of EPA and DHA in feed for European sea bass ( L.).
in Aquaculture (Amsterdam, Netherlands)
Betancor MB
(2018)
Oil from transgenic Camelina sativa containing over 25 % n-3 long-chain PUFA as the major lipid source in feed for Atlantic salmon (Salmo salar).
in The British journal of nutrition
Bianchetti R
(2020)
Phytochrome-Dependent Temperature Perception Modulates Isoprenoid Metabolism.
in Plant physiology
Broughton R
(2018)
New insights in the composition of wax and sterol esters in common and mutant sunflower oils revealed by ESI-MS/MS.
in Food chemistry
Bryant FM
(2019)
Basic LEUCINE ZIPPER TRANSCRIPTION FACTOR67 Transactivates to Establish Primary Seed Dormancy in Arabidopsis.
in The Plant cell
Carrier DJ
(2019)
Mutagenesis separates ATPase and thioesterase activities of the peroxisomal ABC transporter, Comatose.
in Scientific reports
| Description | WP1.1 Understanding and manipulating the regulation of metabolism Johnathan Napier, Frederica Theodoulou, Richard Haslam, Smita Kurup This ISPG WP aims to advance and exploit fundamental knowledge of plant metabolism, to expand the value chains of existing crops, predominantly focused on the niche crop, Camelina. Work Package 1: High-value lipids for health and industry WP1.1 Understanding and manipulating the regulation of metabolism 1.1.1 Optimisation of spatiotemporal expression: To identify factors associated with high accumulation of non-native fatty acids, high-resolution maps of the wild-type and engineered Camelina seeds lipidome have been generated via mass spectrometry imaging (MSI) and compared with radicle and cotyledon profiles obtained by conventional lipidomics. These data define, for the first time, spatial and temporal resolution of novel long chain polyunsaturated fatty acid (LC-PUFA) seed oil biosynthesis. Integration of this information with transcriptome data using intersecting set analysis has identified 20 candidate oil assembly genes as targets to optimize tissue-specific endogenous metabolism. These genes (which would have been difficult to identify in the absence of a combined experimental and bioinformatic analysis) are currently being introduced into elite GM Camelina backgrounds to evaluate their effect on novel fatty acid accumulation. In parallel, endogenous oil assembly pathways are being manipulated in elite germplasm by multiplex CRISPR/Cas9 gene editing (in collaboration with INRA Paris). This workflow paves the way to improve yield of other novel lipids. 1.1.2 RNA export and transgene stability: Studies on the TREX-2 complex in Arabidopsis have confirmed a role in transgene expression, though this may be cis-independent and instead influenced by the genomic site of integration. The role of different 3'-untranslated regions on transgene-derived transcript stability was systematically investigated with a panel of constructs, which have now been introduced in Camelina and are undergoing field evaluation. 1.1.3 manipulating targeted protein degradation for crop improvement: we have developed tandem fluorescent protein timers (tFTs) as a tool for measurement of protein lifetime in planta (Zhang et al., 2019, Plant Physiol. 180, 718-731). We have used tFTs to identify and characterise E3 ligases of the N-recognin pathway (which influences lipid metabolism and several important agronomic traits). Through quantitative proteomic analysis, a role for an N-recognin E3 ligase in plant-pathogen interactions has been identified which will be taken forward in uplift projects. WP1.2 Optimising crop traits for product delivery Two approaches to identify or generate optimal germplasm have been undertaken. Constructs for LC-PUFA production were introduced into six Camelina sativa cultivars selected to represent a range of natural diversity and we have established links with AAFC Saskatoon and Yield10 Biosciences to facilitate access to additional elite germplasm. However, as no marked improvement upon our standard chassis genotype (Celina) was observed, current efforts are directed towards improving product delivery via crop engineering with a focus on seed size/number and oil yield. Importantly, the omega-3 LC-PUFA trait has now been tested in multiple locations (UK, USA and Canada, partly supported by BBSRC Follow-on funding), showing stability over a range of environmental and management conditions. In some cases, the introduction of multigene constructs in Camelina has demonstrated a negative impact on oil yield. Lipidomic analysis of developing seed indicated that this yield penalty is established early in seed/embryo development. A bioinformatic workflow has been designed to integrate a lipidomic/transcriptomic analysis, to provide an unbiased approach to uncover the mechanisms controlling lipid metabolism during seed maturation. This has been complemented by a targeted approach, in which Camelina lines expressing a combination of six transgenes exhibited ~20% increase in seed oil content, partially due to increased seed size. This phenotype has been validated in multiple independent lines over two generations grown in the controlled environment chamber and the lines assessed in the 2019 GM field trial. Candidate seed number regulatory genes have been identified. Proof of concept in Arabidopsis demonstrated increased ovule numbers by overexpressing brassinosteroid biosynthesis or signalling genes- the first evidence of the role of this hormone in controlling seed number. In collaboration with Cambridge, we have discovered a new plant lipid biosynthetic pathway that feeds arbuscular mycorrhizal fungi (Luginbuehl et al. 2017 Science 356, 1178). We have used ectopic expression of a transcription factor from this pathway to drive the secretion of lipids from plant cells, providing an alternative technology to deliver high-value lipid products, e.g. in roots. One of the enzymes of this pathway has been combined with a second heterologous activity to engineer a new-to-nature oil biosynthetic pathway encoded by a single gene fusion, providing new traits for our the crop pipeline. Via the genetic screen that identified TREX-2 components we have also identified a novel gene that confers drought tolerance and improved architecture in Camelina (patent filed: P239758GB); these traits were validated in the 2018 GM field trial. WP1.3 Novel products Iterative metabolic engineering has confirmed the utility of Camelina for delivery of high-value polyunsaturated fatty acids. Using the BBSRC-supported GeneMill synthetic biology foundry, a large library of parts and constructs has been generated by automated MoClo assembly and an efficient "gene-to-field" pipeline established for prioritised transformation to validate plants for further scale-up (such as larger trials, feeding studies etc) and associated impact. To deliver a precisely defined Camelina oil matching the fatty acid profile of oceanic fish oil, further iterations of GM Camelina expressing genes for the omega-3 LC-PUFA pathway have been developed and evaluated for trait stability and agronomic performance in two new GM field trials (18/R08/01 and 19/R08/01). These superior lines contained ~40% omega-3 LC-PUFAs, significantly higher than for the reference fish oil, together with requisite low levels of arachidonic acid but retained levels (~14%) of linoleic acid above the benchmark of 10%. Thus, further iterations will be tested from our library to identify optimal gene combinations. We also demonstrated the feasibility of producing sciadonic acid, a non-native PUFA with pharmaceutical potential in Camelina seed oil via the co-expression of ?5-desaturase with ?9-elongases and ?12-desaturases. Low but significant levels of sciadonic acid in transgenic Camelina seeds indicate the need for optimisation by further iteration, but lipid profiling suggested that acyl exchange was not a bottleneck in SA production, in contrast to the model system Arabidopsis thaliana. Therefore, RNA-seq analysis of developing and mature, sciadonic acid-rich Anemone leveillei seeds has been performed to identify additional factors required for effective heterologous reconstitution of the biosynthetic pathway in transgenic Camelina. In addition to fine-tuning the metabolic engineering of the PUFA pathway in plants, we have made significant progress in delivering two further groups of lipids. Following our initial proof-of-concept work in Arabidopsis demonstrating multi-gene metabolic engineering to produce structured triacylgycerols for better infant nutrition, we have explored translation into three oil-producing platforms: Camelina, oilseed rape and the oleaginous yeast Yarrowia lipolytica. We have been able to match closely both the fatty acid profile and structure of human milk fat in Y. lipolytica and oilseed rape. Pilot-scale extraction and purification procedures have been tested in order to develop wax esters as a source of biolubricants. Wax esters were efficiently (80%) recovered from Camelina oil using molecular distillation and further enrichment could be achieved using hydrogenation and winterization. A preliminary scaled-up extraction/purification experiment was conducted with industrial collaborator, PPM-Magdeburg, leading to funding from the German government to advance the technology readiness level of this project. A second field trial to generate sufficient seed for extraction and performance testing was drilled in May 2019. For the development of lines with novel wax ester compositions (in demand from industrial partners), four branched-chain and hydroxylated fatty acid biosynthetic genes have been introduced into our into our elite Camelina line. |
| Exploitation Route | Too early to say at this stage, though it is important to note that all objectives have so far been met and we are on track to deliver those associated with the last two years of this programme. Since these outputs map onto our Impact Plan, there are good prospects for realizing tangible outcomes and impact from this research. |
| Sectors | Agriculture, Food and Drink,Healthcare,Manufacturing, including Industrial Biotechology,Pharmaceuticals and Medical Biotechnology |
| Description | Contribution to the on-going debate as how to regulate GM and genome edited crops in Europe and post-Brexit Europe. This involves discussions with DEFRA representatives close to the Minister responsible for this area. Notably, the ISPG has played a leading role in pioneering increasingly sophisticated GM field trials, and in public dialogue around GM and GE. |
| First Year Of Impact | 2017 |
| Sector | Agriculture, Food and Drink |
| Impact Types | Economic,Policy & public services |
| Description | Presentation at House of Lord Select Committee |
| Geographic Reach | Local/Municipal/Regional |
| Policy Influence Type | Gave evidence to a government review |
| Description | Ted talk on plant biotechnology and crop genetic improvement: Department for International Development (DFID), Agriculture Research Team. |
| Geographic Reach | Multiple continents/international |
| Policy Influence Type | Influenced training of practitioners or researchers |
| Description | BBSRC sLoLa Brassica Rapeseed And Vegetable Optimisation |
| Amount | £3,514,227 (GBP) |
| Funding ID | BB/P003095/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 01/2017 |
| Description | Brassica Rapeseed And Vegetable Optimisation |
| Amount | £3,514,227 (GBP) |
| Funding ID | BB/P003095/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 01/2017 |
| End | 12/2021 |
| Description | Development of non-glaucous Brassica napus lines by genome editing for enhancing pathogen resistance and reducing chemical sprays. |
| Amount | 4,119,484 kr (SEK) |
| Organisation | Swedish Research Council for Environment, Agricultural Sciences and Spatial Planning (FORMAS) |
| Sector | Private |
| Country | Sweden |
| Start | 03/2018 |
| End | 02/2022 |
| Description | Functional Food and Pharma Ingredients from the Physical Dismantling of Oilseed Rape Oleosomes |
| Amount | £98,212 (GBP) |
| Funding ID | BB/R506278/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 03/2018 |
| End | 02/2022 |
| Description | Genetic improvement of rice seed vigour for dry direct-seeded conditions |
| Amount | £520,445 (GBP) |
| Funding ID | BB/P023428/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 05/2017 |
| End | 04/2019 |
| Description | Genomics-led improvement of biotic and abiotic stress tolerance in mustard rape for economic and environmental sustainability |
| Amount | £1,764,019 (GBP) |
| Funding ID | BB/R019819/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 09/2018 |
| End | 08/2021 |
| Description | International Exchanges 2018 Cost Share (Argentina, France and Russia only) |
| Amount | £10,670 (GBP) |
| Funding ID | IEC\R2\181074 |
| Organisation | The Royal Society |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 12/2018 |
| End | 11/2020 |
| Description | Mass Spectrometry Based Lipidomics and Metabolomics to Drive Bioscience Discovery |
| Amount | £750,000 (GBP) |
| Funding ID | BB/S019588/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | |
| End | 04/2022 |
| Description | Mass Spectrometry Based Lipidomics and Metabolomics to Drive Bioscience Discovery |
| Amount | £750,000 (GBP) |
| Funding ID | BB/S019588/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | |
| End | 03/2022 |
| Description | New varieties of direct seeded rice for farmers in Lower Middle Income Countries |
| Amount | £602,712 (GBP) |
| Funding ID | EP/T015551/1 |
| Organisation | Engineering and Physical Sciences Research Council (EPSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 09/2019 |
| End | 03/2021 |
| Description | Novel Omega-3 Sources in Feeds and Impacts on Salmon Health |
| Amount | £240,067 (GBP) |
| Funding ID | BB/S006400/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 01/2019 |
| End | 12/2021 |
| Description | Nutrition and abiotic stress tolerance in Tomato |
| Amount | £25,000 (GBP) |
| Organisation | Innovate UK |
| Sector | Public |
| Country | United Kingdom |
| Start | 01/2018 |
| End | 03/2019 |
| Description | Oilseed Rape Genetic Improvement Network (OREGIN) phase 5 |
| Amount | £850,000 (GBP) |
| Organisation | Department For Environment, Food And Rural Affairs (DEFRA) |
| Sector | Public |
| Country | United Kingdom |
| Start | 03/2018 |
| End | 03/2023 |
| Description | Production of the lipid-soluble antioxidant Canolol in cruciferous oilseeds - BBSRC HVCfP NIBB PoC |
| Amount | £39,026 (GBP) |
| Organisation | University of York |
| Sector | Academic/University |
| Country | United Kingdom |
| Start | 04/2017 |
| End | 01/2018 |
| Description | RCUK-CIAT Newton Fund: Exploiting biodiversity in Brachiaria/Panicum tropical forage grasses using genetics to improve livelihoods and sustainability |
| Amount | £150,692 (GBP) |
| Funding ID | BB/R022828/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 04/2018 |
| End | 03/2019 |
| Description | Rutherford Fund |
| Amount | £12,000 (GBP) |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 07/2018 |
| End | 12/2018 |
| Description | Rutherford fellowship - flexible talent mobility award |
| Amount | £11,000 (GBP) |
| Funding ID | BB/R506503/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 07/2018 |
| End | 12/2018 |
| Description | Seeding Catalyst Awards |
| Amount | £10,000 (GBP) |
| Funding ID | BB/SCA/Rothamsted/17 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 11/2017 |
| End | 02/2018 |
| Description | Sustainable sources of omega-3 fish oils |
| Amount | £150,000 (GBP) |
| Organisation | AstraZeneca |
| Sector | Private |
| Country | United Kingdom |
| Start | 04/2019 |
| End | 12/2021 |
| Description | UK-Brazil partnership BBSRC/FAPESP. Validation of Arabidopsis thaliana as a suitable model system for investigating the dual protein-lipid antigenic response required for sensitisation to Brazil Nut Ber e 1 |
| Amount | £18,000 (GBP) |
| Funding ID | BBS/OS/NW/000001 |
| Organisation | Newton Fund |
| Sector | Public |
| Country | United Kingdom |
| Start | 07/2018 |
| End | 12/2018 |
| Description | UK-China Centre for the Sustainable Intensification of Agriculture (CSIA) |
| Amount | £500,000 (GBP) |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 04/2018 |
| End | 03/2019 |
| Description | UNTWIST (Uncover and promote tolerance to temperature and water stress in Camelina sativa) |
| Amount | £5,000,000 (GBP) |
| Funding ID | SFS-30-2018-2019-2020 Agri |
| Organisation | European Union |
| Sector | Public |
| Country | European Union (EU) |
| Start | 09/2020 |
| End | 09/2025 |
| Description | Utility of a plant hydrolase as an industrial biocatalyst - |
| Amount | £15,929 (GBP) |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 04/2018 |
| End | 09/2018 |
| Description | Utility of a plant hydrolase as an industrial biocatalyst - BBSRC HVCfP NIBB PoC |
| Amount | £15,929 (GBP) |
| Organisation | University of York |
| Sector | Academic/University |
| Country | United Kingdom |
| Start | 04/2018 |
| End | 09/2018 |
| Description | What determines protein abundance in plants? |
| Amount | £3,354,456 (GBP) |
| Funding ID | BB/T002182/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 11/2019 |
| End | 10/2024 |
| Title | A live imaging system of measuring etiolated seedling growth |
| Description | A live imaging system of measuring etiolated seedling growth |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2018 |
| Provided To Others? | No |
| Impact | We have developed a time-lapse image capture system for measuring the rate of rice seed germination and seedling growth in the dark. The purpose of this technology is to quantify phenotypic variation in seed/seedling vigour in order to assist in genetic improvement for rice for direct seeding. The system can also be applied to other crops in which early vigour is important. |
| Title | Click-chemistry for in vivo labelling of membrane lipids |
| Description | Click-chemistry for in vivo labelling of membrane lipids |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2019 |
| Provided To Others? | No |
| Impact | Uptake of propargylcholine, followed by click chemistry with fluorescein or Alexa Fluor 594 azide was used to visualize choline phospholipids in cells of root, leaf, stem, silique and seed tissues from Camelina. PC tunover and accumulation can be further determine by mass spectrometry. |
| Title | GWAS and gene discovery pipeline |
| Description | GWAS and gene discovery pipeline |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2019 |
| Provided To Others? | Yes |
| Impact | Facilitate rapid and accurate discovery of gene of interest controlling various agronomic traits |
| Title | High resolution accurate mass lipidomics for plants |
| Description | Investment by Rothamsted in a accurate mass Thermo Q Exactive Plus MS has updated and extended analytical capacity. A lipidomics workflow for the Q Exactive has been developed, incorporating an MTBE optimised sample extraction and UPLC method, the Q Exactive Instrument Method, LipidSearch software workflow and lipidomics quality control. We have optimised for identification, alignment and quantitation of all plant lipid species. We have deployed the new instrument to investigate tissue-specific novel fatty acid assembly into seed oil. See: Haslam, R.P. and Feussner, I. (2017) Green light for lipid fingerprinting. BBA Molecular and Cell Biology of Lipids 1862(8):782-785. Commissioning the new instrumentation has provided the ISPG with increased analytical capacity, efficiency, responsiveness and flexibility. Moreover, it has enabled the development of a new workflow, lipid fingerprinting, that integrates the advantages of shotgun lipidomics (quantitative data) with LC-MS-based strategies (higher resolution and/or coverage). |
| Type Of Material | Improvements to research infrastructure |
| Year Produced | 2017 |
| Provided To Others? | Yes |
| Impact | The enhanced analytical capability provide by this instrumentation has enabled the development of a new workflow, lipid fingerprinting, that integrates the advantages of shotgun lipidomics (quantitative data) with LC-MS-based strategies (higher resolution and/or coverage). Collectively, the instrumentation and associated workflows enable the development of new knowledge-based strategies to redesign lipid pathways for the sustainable production of high value products. |
| Title | High throughput HPLC-ESI-MS/MS profilling method for analysis of free and esterified sterols in biological samples |
| Description | We have developed a high throughput HPLC/ELSD-ESI-MS/MS method for the compositional and content analysis of seed sterols and steryl esters in a B. napus diversity collection (over 400 accessions representing the ASSYST panel). 1600 samples analysed. This methods expanded the analytical capability of the state of the art lipidomic platform available at Rothamsted Research. |
| Type Of Material | Improvements to research infrastructure |
| Year Produced | 2017 |
| Provided To Others? | Yes |
| Impact | This methods has been extented to include detectection of wax esters as well as sterol esters in seed oil samples. This allowed the accurate compositional analysis of the ester fraction in commercial sunflower oil as well as in mutant varieties. This work may have implications for vegetable oil treatment and authentication allowing both the reduction of oil turbidity at room temperature (wax esters) and the detection of fraudulent mixture of high value oils with cheaper and lower quality oils (steryl esters markers). This work was submitted to food Chemistry and is curently under review: New insights in the composition of Wax and Sterol Esters in common and mutant sunflower oils revealed by ESI-MS/MS. Richard Broughton, Noemi Ruíz-Lopez, Enrique Martínez-Force, Rafael Garcés, Fred Beaudoin* and Joaquín J. Salas* (2018). |
| Title | Tandem fluorescent timers for protein lifetime measurement in plants |
| Description | A tandem fluorescent protein timer (tFT) is a fusion of two different fluorescent proteins with distinct fluorophore maturation kinetics, which enable protein age to be estimated from the ratio of fluorescence intensities of the two fluorescent proteins. We have adopted this technology (originally developed in yeast) for use in plants. tFTs expressed in transient systems enable efficient screens for relative protein lifetime, useful for testing the effects of mutations and different genetic backgrounds on protein stability. Stably expressed tFTs can be used to study native protein dynamics with high temporal resolution in response to exogenous or endogenous stimuli. |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2019 |
| Provided To Others? | Yes |
| Impact | The method has only just been published so it is too early to judge impact but conference presentations have led to other researchers adopting the method and we are aware of at least one study submitted for publication. |
| Description | Analysis of the Wax and Steryl Ester content in vegetable oils. Implications for oil processing, authentification and commercialisation. |
| Organisation | Instituto de la Grasa |
| Country | Spain |
| Sector | Private |
| PI Contribution | Lipidomic profiling of oil minor components (wax and lipid soluble bioactives) GC-FID, GC-MS, ESI-MS/MS |
| Collaborator Contribution | Oil crops cultivation and harvesting Oil extraction, testing of biophysical properties |
| Impact | one research article in Food sciences |
| Start Year | 2017 |
| Description | Collaboration with DR Tara Hessa |
| Organisation | Stockholm University |
| Country | Sweden |
| Sector | Academic/University |
| PI Contribution | Lipidomic profiling of mouse retina |
| Collaborator Contribution | Visting worker for lipid extraction |
| Impact | Ongoing work |
| Start Year | 2019 |
| Description | Collaboration with Dr Arvind Kumar |
| Organisation | International Rice Research Institute |
| Country | Philippines |
| Sector | Charity/Non Profit |
| PI Contribution | Rice seedlings phenotyping, GWAS for direct seeding |
| Collaborator Contribution | To be entered |
| Impact | Ongoing work |
| Start Year | 2017 |
| Description | Collaboration with Dr Graham Burdge |
| Organisation | University of Southampton |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Lipidomic profiling of human serum lipids |
| Collaborator Contribution | Serum lipid extracts from human trial testing fish oil and O3 camelina oil |
| Impact | Ongoing work |
| Start Year | 2019 |
| Description | Collaboration with Dr Kenneth Tong |
| Organisation | University College London |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Re-engineering the biomechanics of seedling emergence to enhance crop performance (RICE+) |
| Collaborator Contribution | To be entered |
| Impact | Ongoing work |
| Start Year | 2018 |
| Description | Collaboration with Dr Nitika Sandhu |
| Organisation | Punjab Aricultural University |
| Country | India |
| Sector | Academic/University |
| PI Contribution | Rice seedlings phenotyping, GWAS for direct seeding |
| Collaborator Contribution | To be entered |
| Impact | Ongoing work |
| Start Year | 2017 |
| Description | Collaboration with Prof Fernando Carrari |
| Organisation | University of Buenos Aires |
| Country | Argentina |
| Sector | Academic/University |
| PI Contribution | Lipidomic profiling of Tomato variation in response to heat stress |
| Collaborator Contribution | To be entered |
| Impact | Ongoing work |
| Start Year | 2019 |
| Description | Collaboration with Prof John Harwood |
| Organisation | Cardiff University |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Role of the lipidome on cold stress tolerance |
| Collaborator Contribution | Tracking expression of transcriptin factors |
| Impact | Ongoing work |
| Start Year | 2019 |
| Description | Collaboration with Prof jean-Dennis Faure |
| Organisation | French National Institute of Agricultural Research |
| Country | France |
| Sector | Academic/University |
| PI Contribution | Selection of candidate genes and screening of generations |
| Collaborator Contribution | production of muliplex CRISPR lines in TAG assembly - Wt and elite camelina germplasm |
| Impact | Ongoing work |
| Start Year | 2019 |
| Description | Composition and properties of B napus oleosome ghosts for use as a structuring and functional food ingredient |
| Organisation | Cargill Europe |
| Country | Belgium |
| Sector | Private |
| PI Contribution | Students and PDRAs training in lipid biochemistry analytical methods. Lipidomic analyses |
| Collaborator Contribution | Extraction, purification of OSR oil bodies, biophysical properties testing. |
| Impact | joint RRes-UoN iCASE PhD studentship |
| Start Year | 2017 |
| Description | Composition and properties of B napus oleosome ghosts for use as a structuring and functional food ingredient |
| Organisation | University of Nottingham |
| Department | University of Nottingham Museum |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Students and PDRAs training in lipid biochemistry analytical methods. Lipidomic analyses |
| Collaborator Contribution | Extraction, purification of OSR oil bodies, biophysical properties testing. |
| Impact | joint RRes-UoN iCASE PhD studentship |
| Start Year | 2017 |
| Description | Development of non-glaucous Brassica napus lines by genome editing |
| Organisation | Swedish University of Agricultural Sciences |
| Country | Sweden |
| Sector | Academic/University |
| PI Contribution | Biochemical and physiological characterisation of genome edited B.napus lines |
| Collaborator Contribution | generation of genome edited B. napus lines |
| Impact | work in progress |
| Start Year | 2018 |
| Description | Development of tandem fluorescent protein timer methodology |
| Organisation | Heidelberg University |
| Department | Centre for Organismal Studies |
| Country | Germany |
| Sector | Academic/University |
| PI Contribution | Co-development of research methodology |
| Collaborator Contribution | Co-development of research methodology |
| Impact | A joint publication is reported in the appropriate section |
| Start Year | 2017 |
| Description | Dr James Doughty, studentship |
| Organisation | University of Bath |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Increasing seed yields by the targeted modulation of brassinosteroids |
| Collaborator Contribution | To be entered |
| Impact | Ongoing |
| Start Year | 2018 |
| Description | Engineering novel seed storage lipids - ketocarotenoids and esters |
| Organisation | University of Nebraska-Lincoln |
| Country | United States |
| Sector | Academic/University |
| PI Contribution | Synthesis of genes for assembly and expression in transgenic plants - reciprocal exchange of transgenic material for environmental release (GM field trials) |
| Collaborator Contribution | Synthesis of genes for assembly and expression in transgenic plants - reciprocal exchange of transgenic material for environmental release (GM field trials) |
| Impact | None in the public domain. |
| Start Year | 2017 |
| Description | Evaluation of a plant hydrolase as an industrial enzyme |
| Organisation | Protein Technologies Ltd |
| Country | United Kingdom |
| Sector | Private |
| PI Contribution | We produced and supplied a recombinant plant hydrolase with a novel activity for application testing by Dr Dietmar Lang at Unilever R&D. |
| Collaborator Contribution | Unilever R&D carried out application testing in-house. |
| Impact | Work was supported by BBSRC HVCfP NIBB PoC grant. Material still being tested. |
| Start Year | 2018 |
| Description | Evaluation of a plant hydrolase as an industrial enzyme |
| Organisation | Unilever |
| Department | Unilever UK R&D Centre Port Sunlight |
| Country | United Kingdom |
| Sector | Private |
| PI Contribution | We produced and supplied a recombinant plant hydrolase with a novel activity for application testing by Dr Dietmar Lang at Unilever R&D. |
| Collaborator Contribution | Unilever R&D carried out application testing in-house. |
| Impact | Work was supported by BBSRC HVCfP NIBB PoC grant. Material still being tested. |
| Start Year | 2018 |
| Description | Field evaluation of gene-edited crops |
| Organisation | French National Institute of Agricultural Research |
| Department | INRA Versailles |
| Country | France |
| Sector | Academic/University |
| PI Contribution | Obtaining regulatory approval to carry out environmental release (field trial) of gene-edited camelina - this was the first release of it's kind in Europe. |
| Collaborator Contribution | Provision of characterised, gene-edited material for release |
| Impact | A paper was published eLife. |
| Start Year | 2018 |
| Description | Functional and biochemical characterisation of VLC-PUFA biosynthetic gene/enzyme candidates in yeast and Algae |
| Organisation | University of Huelva |
| Country | Spain |
| Sector | Academic/University |
| PI Contribution | cloning and functional characterisation of Algal Elongase genes in yeast Lipid analyses, GC-FID, GC-MS, ESI-MS/MS |
| Collaborator Contribution | Student/PDRA visiting workers Cultivation and analysis of algal strains |
| Impact | Research article in preparation |
| Start Year | 2018 |
| Description | Genetic improvement of Brassica oilseed crops |
| Organisation | University of York |
| Department | Biological Sciences |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Extraction, purification and analysis of lipids (biomass surface lipids, seed oil and oil minor components (wax esters and lipid soluble bioactives) Training of students and PDRAs in lipid biochemistry analytical techniques |
| Collaborator Contribution | Establishment of Brassica crop genomic resources Generation of a gamma-ray mutagenized B. napus population Screening and selection of mutagenized lines |
| Impact | RIPR and BBSRC-DBT Newton-PORI research fish entries |
| Start Year | 2017 |
| Description | Identification and characterization of a plant lipid biosynthesis pathway that supplies arbuscular mycorrhizal fungi with lipids |
| Organisation | University of Cambridge |
| Department | The Sainsbury Laboratory |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | We characterised the enzyme activity of the RAM2 protein from Medicago truncatula, hypothesised that it played a role supplying lipids to arbuscular mycorrhizal fungi and devised a series of experiments involving plant expression for marker fatty acids and radiolabeling of mutants to prove this hypothesis. |
| Collaborator Contribution | Our partner (Prof Giles Oldroyd) first identified the RAM2 gene through a genetic screen, along with RAM1, characterised the mutants and demonstrated that RAM1 induced RAM2, as well as a other genes putatively involved in fatty acid and lipid biosynthesis and transport. Our partner also generated constructs and transgenic lines to allow us to carry out experiments to show that arbuscular mycorrhizal fungi receive lipids from their host plant. |
| Impact | We published our work in 2017 (doi: 10.1126/science.aan0081). |
| Start Year | 2015 |
| Description | Investigating the dual protein-lipid antigenic response required for sensitisation to Brazil Nut Ber e 1 |
| Organisation | Universidade de São Paulo |
| Country | Brazil |
| Sector | Academic/University |
| PI Contribution | Synthesis and cloning of WT and mutant form of the Ber-e-1 gene. Camelina transformation Brazil nut lipid extraction and purification |
| Collaborator Contribution | In vitro assays with T-cells (UoN). Purified protein with and without lipid fractions Sensitization assays in vivo (in rats) |
| Impact | research manuscript in preparation |
| Start Year | 2018 |
| Description | Investigating the dual protein-lipid antigenic response required for sensitisation to Brazil Nut Ber e 1 |
| Organisation | University of Nottingham |
| Department | School of Biosciences |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Synthesis and cloning of WT and mutant form of the Ber-e-1 gene. Camelina transformation Brazil nut lipid extraction and purification |
| Collaborator Contribution | In vitro assays with T-cells (UoN). Purified protein with and without lipid fractions Sensitization assays in vivo (in rats) |
| Impact | research manuscript in preparation |
| Start Year | 2018 |
| Description | Investigating the role of rice receptor kinases in lipid supply to arbuscular mycorrhizal fungi. |
| Organisation | University of Cambridge |
| Department | Department of Plant Sciences |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | We are analysing lipids and metabolism in the mutants to determine if they are defective in lipid supply to the fungus. |
| Collaborator Contribution | Our partner (Dr Uta Paszkowski) has identified receptor kinase mutants in rice that are weakly impaired in arbuscular mycorrhization but have a dramatic reduction in the formation of vesicles that store lipids. |
| Impact | Not yet. Work in progress. |
| Start Year | 2018 |
| Description | MIC of transgenic microalgae expressing antimicrobial peptides |
| Organisation | National Physical Laboratory |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Production of transgenic starins of microalgae expressing antimicrobial peptides |
| Collaborator Contribution | Measurement of inhibition activities of the transgenic strains |
| Impact | Ongoing work |
| Start Year | 2019 |
| Description | Metabolic and biochemical characterisation of cytosolic lipid droplets that appear to be associated with chronic kidney disease in domestic cat |
| Organisation | University of Nottingham |
| Department | School of Veterinary Medicine and Science Nottingham |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Lipidomic profiling of lipid droplets from wild and domestic cat kidneys GC-FID, GC-MS, HPLC,ESI-MS/MS Training of PhD student in lipid biochemistry analytical methods. |
| Collaborator Contribution | Subcellular fractionation of animal tissue extraction and purification of lipid droplets intervention studies in domestic cat (analysis of correlation between nutrition and chronic diseases) |
| Impact | on-going work |
| Start Year | 2018 |
| Description | Microcin expression |
| Organisation | Ingenza Ltd |
| Country | United Kingdom |
| Sector | Private |
| PI Contribution | Production of microcin in host strains |
| Collaborator Contribution | To be entered |
| Impact | Ongoing work |
| Start Year | 2019 |
| Description | Optimising the processing of novel oils and wax esters |
| Organisation | Pilot Pflanzenöltechnologie Magdeburg |
| Country | Germany |
| Sector | Academic/University |
| PI Contribution | Provision of novel (GM) material containing non-native wax esters |
| Collaborator Contribution | Extraction and evaluation of novel wax esters from GM plant seeds |
| Impact | . |
| Start Year | 2017 |
| Description | Plant wax esters as biolubricants |
| Organisation | Cargill |
| Country | United States |
| Sector | Private |
| PI Contribution | Provision of novel non-GM germplasm for evaluation and extraction |
| Collaborator Contribution | Deployment of proprietary know-how and capabilities to assess potential new product leads |
| Impact | None yet |
| Start Year | 2017 |
| Description | Production of human milk fat substitute in oilseed rape |
| Organisation | Cargill |
| Country | United States |
| Sector | Private |
| PI Contribution | We generated gene constricts and genetically transformed proprietary oilseed rape material provided by Kristin Gray at Cargill in order to produce seeds containing oil with the same sterioisomeric structure as human milk fat. |
| Collaborator Contribution | Cargill provided their proprietary oilseed rape material and genetic markers for their HOLL trait. |
| Impact | None yet. Material still to be fully characterised. |
| Start Year | 2018 |
| Description | Production of human milk fat substitute in oilseed rape |
| Organisation | National Institute of Agronomy and Botany (NIAB) |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | We generated gene constricts and genetically transformed proprietary oilseed rape material provided by Kristin Gray at Cargill in order to produce seeds containing oil with the same sterioisomeric structure as human milk fat. |
| Collaborator Contribution | Cargill provided their proprietary oilseed rape material and genetic markers for their HOLL trait. |
| Impact | None yet. Material still to be fully characterised. |
| Start Year | 2018 |
| Description | Quantitative proteomics for analysis of targeted protein degradation |
| Organisation | University of Cambridge |
| Department | Cambridge Centre for Proteomics |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Provision of plant biology and protein labelling expertise |
| Collaborator Contribution | Provision of mass spectrometry expertise |
| Impact | Joint publications are listed the relevant section. |
| Start Year | 2013 |
| Description | Rothamsted Research- CSIRO Linkage proposals, Collaboration with Dr Matthew Nelson |
| Organisation | Commonwealth Scientific and Industrial Research Organisation |
| Country | Australia |
| Sector | Public |
| PI Contribution | To be entered |
| Collaborator Contribution | To be entered |
| Impact | Ongoing work |
| Start Year | 2019 |
| Description | Synthetic Biology - Foundry validation |
| Organisation | University of Liverpool |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Design, synthesis and conceptualisation of modular multi-component plant transformation vectors for advanced metabolic engineering |
| Collaborator Contribution | Optimise and validate the combinatorial assembly of multi-gene constructs for plant transformation. |
| Impact | None in the public domain yet. |
| Start Year | 2018 |
| Description | new collaboration |
| Organisation | University of Bath |
| Department | Department of Biology and Biochemistry |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | A new PhD project on 'Increasing seed yields by the targeted modulation of brassinosteroids (BR)' has been initiated with the University of Bath (Dr James Doughty). Recent preliminary research in the Kurup lab identified novel BR related loci that play a role in increased ovule primordia. We have generated transgenic lines in Arabidopsis that have increased ovule number in their gynoecia compared to control plants. This collaboration is to further investigate these findings. |
| Collaborator Contribution | The Bath partners will help with the characterisation of the above phenotypes, in particular investigations on the development of post zygotic barriers, if any in these lines. They will also help with semi-restricted seed yield experiments. |
| Impact | This project will include a variety of disciplines- molecular biology, genetics, developmental cell biology, imaging, and bioinformatics. The collaboration outlined in this proposal is designed to benefit from the complimentary expertise and experimental resources within each institution. |
| Start Year | 2017 |
| Title | Desaturase And Method For The Production Of Polyunsaturated Fatty Acids In Transgenic Organisms |
| Description | he present invention relates to a polynucleotide from Emiliana huxleyi which codes for a desaturase and which can be employed for the recombinant production of polyun- saturated fatty acids. The invention furthermore relates to vectors, host cells and transgenic nonhuman organisms which comprise the polynucleotide according to the invention, and to the polypeptides encoded by the polynucleotide. The invention furthermore relates to antibodies against the polypeptide according to the invention. Finally, the invention also relates to production methods for the polyunsaturated fatty acids and for oil, lipid and fatty acid compositions and to their use as drugs, cosmetics, foodstuffs, feedstuffs, preferably fish food, or food supplements. |
| IP Reference | CA2723072 |
| Protection | Patent granted |
| Year Protection Granted | 2017 |
| Licensed | Commercial In Confidence |
| Impact | . |
| Title | Genetically modified plants with enhanced drought tolerance |
| Description | This invention relates to plants with improved drought tolerance, leading to improved yields of crop plants under variable field conditions. The plants are genetically modified plants |
| IP Reference | P239758GB/RAW |
| Protection | Patent application published |
| Year Protection Granted | 2019 |
| Licensed | Commercial In Confidence |
| Impact | The modified plant materials and germplasm have potential application in the generation and breeding of new more drought resistant plants, including modification of existing plant varieties to improve field performance. |
| Title | Novel fatty acid desaturases and elongases and uses thereof |
| Description | The invention provides isolated nucleic acid molecules which encode novel fatty acid desaturases and elongases from the organism Emiliana huxleyi. The invention also provides recombinant expression vectors containing desaturase or elongase nucleic 5 acid molecules, host cells into which the expression vectors have been introduced, and methods for large-scale production of long chain polyunsaturated fatty acids (LCPUFAs), e.g. arachidonic acid (ARA), eicosapentaenoic acid (EPA) or docosahexaenoic acid (DHA). 3068284_1 (GHMatters) P89302.AU |
| IP Reference | AU2018282290 |
| Protection | Patent application published |
| Year Protection Granted | 2019 |
| Licensed | Commercial In Confidence |
| Impact | This patent protects the discover of an acyl-CoA dependent D5-desaturase, a key step in the production of EPA in transgenic plants and algae. The patent covers the use of this gene in both transgenic systems. |
| Title | Process For Producing A Polyunsatured Fatty Acid Comprising Arachidonic Acid |
| Description | A process for producing a polyunsaturated fatty acid (PUFA) comprising arachidonic acid (ARA) wherein the process comprises the conversion of linoleic acid to arachidonic acid in a non-human transgenic organism, the process comprising (i) providing a non-human transgenic organism comprising, as heterologous nucleic acid sequence, at least one nucleic acid sequence which encodes a polypeptide with ?9- elongase activity and which is selected from the group consisting of: a) a sequence comprising nucleic acid residues 7668 to 9200 of SEQ ID NO: 1; b) a nucleic acid sequence which hybridizes under stringent conditions with a nucleic acid sequence comprising residues 7668 to 9200 of SEQ ID NO: 1, wherein hybridization is in 6 x sodium chloride/sodium citrate (SSC) at approximately 45°C, followed by one or more washing steps in 0.2 x SSC, 0.1% SDS at 50 to 65°C; c) a nucleic acid sequence which encodes a polypeptide comprising SEQ ID NO: 2; d) a nucleic acid sequence which encodes a polypeptide with at least 85% identity at the amino acid level with SEQ ID NO: 2; and at least one nucleic acid sequence encoding a ?8 desaturase and at least one nucleic acid sequence encoding a ?5 desaturase; and (ii) expressing said nucleic acid sequence |
| IP Reference | EP2380984 |
| Protection | Patent granted |
| Year Protection Granted | 2018 |
| Licensed | Commercial In Confidence |
| Impact | Part of the IP portfolio around which commercialisation is focused |
| Title | Production Of Omega-3 Long Chain Polyunsaturated Fatty Acids |
| Description | A recombinant camelina plant or cell comprising one or more polynucleotides encoding a ?6-desaturase, a ?6-elongase and a ?5-desaturase operably linked with one or more regulatory sequences. |
| IP Reference | AU2013246661 |
| Protection | Patent granted |
| Year Protection Granted | 2018 |
| Licensed | Commercial In Confidence |
| Impact | Foundation of an IP portfolio with which to commercialise the omega-3 technology |
| Title | TRANSGENIC MICROALGAE WITH INCREASED PRODUCTION OF AT LEAST ONE OMEGA-3 LONG CHAIN POLYUNSATURATED FATTY ACID |
| Description | The invention relates to genetically modified organisms with enhanced production of omega-3 long chain polyunsaturated fatty acids. |
| IP Reference | US2018312888 |
| Protection | Patent application published |
| Year Protection Granted | 2018 |
| Licensed | Commercial In Confidence |
| Impact | Establishing and validating novel platforms for the production of omega-3 LC-PUFAs |
| Description | 22nd International Symposium on Plant Lipids, Goettingen |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Poster presentation Development of an ESI-MS/MS method for high throughput profiling of free and esterified sterols and Associative Transcriptomic analysis of seed phytosterol content and composition using a B. napus diversity panel Richard Broughton1, Lenka Havlickova2, Andrea Harper2, Ian Bancroft2 and Frédéric Beaudoin1 1Biological Chemistry and Crop Protection Department, Rothamsted Research, Harpenden, AL5 2JQ, UK. 2 Department of Biology, University of York, Heslington, YO10 5DD, UK. Oilseed rape (Brassica napus) is a commercially viable crop owing partly to the seed oil co-products which can be exploited commercially, adding value to the crop. One emerging "bio-refining" opportunity in rapeseed involves the extraction and purification of phytosterols from the oil. Phytosterols are industrially important due to their increasing use as both a functional food component and as nutraceuticals (role in lowering LDL cholesterol). Phytosterols exist in two predominant forms in Brassica napus seeds, free sterols and steryl esters, and it is the latter that is of greater industrial use because of their increased solubility in fat and oil based food products. Although the biosynthetic pathways involved in the synthesis of plant sterols are known to some extent from studies conducted in species such as Arabidopsis thaliana, the quantitative control of their accumulation (key to yield of these compounds) is not understood. In addition, as seed phytosterol content has not been the foci for crop improvement by breeders, there is no improved germplasm or molecular markers available to aid breeding. We have developed a high throughput ESI-MS/MS method for the compositional and content analysis of seed sterols and steryl esters in a B. napus diversity collection (over 400 accessions representing the ASSYST panel). Here we report the quantitative variations observed and the identification of sequence (SNPs) and gene expression markers (GEMs) associated with these traits in both the A and the C genomes. The strong correlation observed between SNPs and GEMs will greatly facilitate the identification and cloning of the underlying causal genes. Interestingly, we found that very long chain fatty acids are completely excluded from the steryl ester fraction, regardless of the seed fatty acid composition, and that steryl ester content strongly correlates with the seed total sterol content. |
| Year(s) Of Engagement Activity | 2016 |
| URL | http://www.eurofedlipid.org/meetings/archive/goettingen2016/ |
| Description | 8th European Symposium on Plant Lipids |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Scientific presentation, Monday 3rd July 2017: High-throughput Profiling of Free and Esterified Sterols and Associative Transcriptomics Analysis of Seed Phytosterol Content and Composition in a B. napus diversity Panel (ASSYST) R. Broughton, Harpenden/GB , L. Havlickova, York/GB, A. Harper, York/GB, I. Bancroft, York/GB, F. Beaudoin, Harpenden/GB |
| Year(s) Of Engagement Activity | 2017 |
| URL | http://www.eurofedlipid.org/meetings/malmoe2017/index.php#programme |
| Description | 8th European Symposium on Plant Lipids, Malmö, Sweden |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | I presented research findings regarding our discovery that plants supply arbuscular mycorrhizal fungi with lipids as a major carbon source. |
| Year(s) Of Engagement Activity | 2017 |
| Description | BBSRC UK Brassica strategy meetings |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Other audiences |
| Results and Impact | Represented Rothamsted a strategic meetings organised by BBSRC to discuss future research and funding relevant to the UK Brassica research community. Presentation of RRes past and current research involving Brassica crops in front of academic and industrial stakeholders and representatives of funding bodies (BBSRC, DEFRA, ADAS, AHBD). |
| Year(s) Of Engagement Activity | 2017,2018 |
| Description | Engagement with industry/stakeholder |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Industry/Business |
| Results and Impact | 2nd Annual BRAVO sLoLa stakeholder meetings where members of the breeding/growing industry discuss progress on the BRAVO project. |
| Year(s) Of Engagement Activity | 2019 |
| Description | Engagement with industry/stakeholder |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Industry/Business |
| Results and Impact | The second annual 'Brassica Rapeseed And Vegetable Optimisation' stakeholder meeting was organised on the 19the of February 2019 at Rothamsted Research. Besides project partners and members of the supervisory board, over 20 stakeholder/companies were invited to the meeting. Along with project presentation, vegetable and oilseed breeders presented challenges faced by them and this was followed by several feedback and discussion sessions. |
| Year(s) Of Engagement Activity | 2019 |
| Description | Engagement with industry/stakeholder |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Industry/Business |
| Results and Impact | Presented by Olga Sayanova outputs from metabolic engineering of microalgae |
| Year(s) Of Engagement Activity | 2017 |
| Description | Engagement with industry/stakeholder |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Industry/Business |
| Results and Impact | Visit and presentation of phenotyping technolgies (by S Kurup) to Plant Impact |
| Year(s) Of Engagement Activity | 2019 |
| Description | Engagement with industry/stakeholder |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Industry/Business |
| Results and Impact | Meeting with Elsoms on several aspects of seed/reproductive research as avenues for future collaborations. |
| Year(s) Of Engagement Activity | 2018 |
| Description | Engagement with industry/stakeholder |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Industry/Business |
| Results and Impact | Visit and presentation of phenotyping technolgies (by S Kurup) to Elsoms |
| Year(s) Of Engagement Activity | 2019 |
| Description | First Annual Stakeholder Meeting |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Industry/Business |
| Results and Impact | The first Annual BRAVO stakeholder meeting was organised on the 1st of February 2018 at Rothamsted Research. Besides project partners and members of the supervisory board, over 21 stakeholder/companies were invited to the meeting. Along with project presentation, vegetable and oilseed breeders presented challenges faced by them and this was followed by several feedback and discussion sessions. |
| Year(s) Of Engagement Activity | 2018 |
| Description | Fish Farmer Article |
| Form Of Engagement Activity | A magazine, newsletter or online publication |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Industry/Business |
| Results and Impact | Fish Farmer Magain - Volume 41 Number 01 January 2018. Article 'Man on a Mission' pg 30-33 |
| Year(s) Of Engagement Activity | 2018 |
| URL | https://issuu.com/fishfarmermagazine/docs/fish_farmer_january_2018 |
| Description | Freddie Theodoulou presentation "Plant Proteostasis; controlling protein stability for sustainable agriculture" |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Freddie Theodoulou presented "Plant Proteostasis; controlling protein stability for sustainable agriculture" at New Phytologist Workshop, University of Durham, UK |
| Year(s) Of Engagement Activity | 2018 |
| Description | Front-cover feature in BBSRC Business magazine |
| Form Of Engagement Activity | A magazine, newsletter or online publication |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Industry/Business |
| Results and Impact | This was a co-ordinated media campaign, working with BBSRC and the BBC to run the story about our GM camelina oil being used in a salmon feeding study in a sea loch. The story was run on multiple platforms, BBC TV, BBC radio and website |
| Year(s) Of Engagement Activity | 2018 |
| URL | https://www.bbc.co.uk/news/science-environment-44743003 |
| Description | Global Omega-3 Forum |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Attendance at GoEd Exchange 2018 - contribute to panel discussion on GM Omega-3 |
| Year(s) Of Engagement Activity | 2018 |
| Description | Grand Challenges Meeting, Washington, DC |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | I was invited to attend the BMG-funded Grand Challenges Meeting, where I participated in the crops session workshop and presented flash talks on our GCRF BBSRC-funded work on improving seed vigour for direct-seeded rice and our work on nutrient exchange in the plant arbuscular mycorrhizal association. |
| Year(s) Of Engagement Activity | 2017 |
| Description | Hosted pupils from 2 secondary schools from Harpenden in Bioimaging |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | Organised by S Kurup, Bioimaging staff introducing imaging technologies (light, confocal, EM) and insights into our research |
| Year(s) Of Engagement Activity | 2017 |
| Description | Hosted pupils from 2 secondary schools from Harpenden in Bioimaging |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | Organised by S Kurup, Bioimaging staff introducing imaging technologies (light, confocal, EM) and insights into our research |
| Year(s) Of Engagement Activity | 2018 |
| Description | Hosted two secondary school pupils from St Albans Girls' School for work shadowing |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | Freddie Theodoulou and Chelsea Rundle hosted two secondary school pupils from St Albans Girls' School for work shadowing |
| Year(s) Of Engagement Activity | 2019 |
| Description | Hosted visit by local school children |
| Form Of Engagement Activity | Participation in an open day or visit at my research institution |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | I hosted a visit by local 6th form school children to investigate Rothamsted Research and the work we do. This involved describing the TPM and other ISPG programs, observing and learning about wheat transformation, EM and light microscopy, field phenotyping facilities, the insect survey and the GM field site and long term experiments. |
| Year(s) Of Engagement Activity | 2018 |
| Description | Hosted visit by local school children |
| Form Of Engagement Activity | Participation in an open day or visit at my research institution |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | I hosted a visit by local 6th form school children to investigate Rothamsted Research and the work we do. This involved describing the TPM and other ISPG programs, observing and learning about wheat transformation, EM and light microscopy, field phenotyping facilities, the insect survey and the GM field site and long term experiments. |
| Year(s) Of Engagement Activity | 2017 |
| Description | Interview with SCIENCE magazine regarding gene editing and ECJ ruling |
| Form Of Engagement Activity | A magazine, newsletter or online publication |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Public/other audiences |
| Results and Impact | I was interviewed by Science magazine for my response to the ECJ ruling on gene editing, based on the fact that I was running the only overt GE field trial in Europe |
| Year(s) Of Engagement Activity | 2018 |
| Description | Interview with WIRED magazine on gene editing |
| Form Of Engagement Activity | A magazine, newsletter or online publication |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Public/other audiences |
| Results and Impact | I was interviewed to provide my view on the recent ECJ court case regarding legal status of gene editing in Europe. Interview was part of a larger item on the potential of gene editing. |
| Year(s) Of Engagement Activity | 2018 |
| URL | https://www.wired.co.uk/article/cavendish-banana-extinction-gene-editing |
| Description | Invited Departmental presentation University of Buenos Aires, Argentina |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Presentation on Engineering plants for nutrition |
| Year(s) Of Engagement Activity | 2017,2019 |
| Description | Invited Departmental presentation University of Buenos Aires, Argentina |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Plant membrane remodelling in response to abiotic stress |
| Year(s) Of Engagement Activity | 2019 |
| Description | Invited Lecture, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Professional Practitioners |
| Results and Impact | Lecture title: Oilseed crops as integrated production platforms for food, feed, fuel and renewable industrial feedstock |
| Year(s) Of Engagement Activity | 2016 |
| Description | Invited Talk ISFNF aquaculture congress |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Johnathan Napier gave an invited talk at the ISFNF aquaculture congress in Gran Canaria |
| Year(s) Of Engagement Activity | 2018 |
| Description | Invited lecture at Plant Proteostasis 2019, Freiburg, Germany |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Freddie Theodoulou gave a presentation on tandem timer technology |
| Year(s) Of Engagement Activity | 2019 |
| Description | Invited lecture at University of York |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Professional Practitioners |
| Results and Impact | Freddie Theodoulou gave a presentation of roles of proteostasis in plants |
| Year(s) Of Engagement Activity | 2018 |
| Description | Invited lecture at Utrecht University |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Freddie Theodoulou gave a presentation of roles of proteostasis in plants |
| Year(s) Of Engagement Activity | 2018 |
| Description | Invited lecture, Institute of Life Sciences Jiangsu University, Zhenjiang, China |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Postgraduate students |
| Results and Impact | Lecture title: Oilseed crops as integrated production platforms for food, feed, fuel and renewable industrial feedstock. 1 h lecture Including a presentation of ther power of Associative transcriptomics for improving the value of co-products in oilseed rape (B. napus). |
| Year(s) Of Engagement Activity | 2016 |
| Description | Invited lecture, Intituto de la Grasa, Seville |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Professional Practitioners |
| Results and Impact | Plant biotechnology and biorefinning lecture: Oleaginous crops as integrated production platforms for sustainable production of food, feed, fuel and renewable industrial feedstock Initiated discussions about future collaboration on transaltional research projects and students exchange/training. |
| Year(s) Of Engagement Activity | 2018 |
| Description | Invited lecture, Workshop on Integrative Plant Biology Wuhan, China |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Huazhong (HZAU) - University of Nebraska Lincoln (UNL) Joint workshop on Integrative Plant Biology: Innovations in Crop Improvement to Meet the Global Grand Challenges of the 21st Century. September 2016 Lecture title: Associative transcriptomic approach to improving the value of co-products in oilseed rape (B. napus). |
| Year(s) Of Engagement Activity | 2016 |
| Description | Invited presentation at "Plant Proteostasis at the N-terminus", Halle, Germany |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Freddie Theodoulou gave a presentation of techniques for studying roles of the N-degron pathway in plant proteostasis |
| Year(s) Of Engagement Activity | 2017 |
| Description | Invited seminar, Department of Plant Biochemistry, Georg-August-Universität, Göttingen |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Postgraduate students |
| Results and Impact | I presented our recent findings that plants supply arbuscular mycorrhizal fungi with lipids as their major carbon source. |
| Year(s) Of Engagement Activity | 2017 |
| Description | Invited seminar/lecture on Plant Biotechnology and biorefining - UNICAMP Sao Paulo. |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Study participants or study members |
| Results and Impact | Lecture on Plant biotechnology and Biorefining illustrated by examples of successful research project carried out at Rothamsted research. |
| Year(s) Of Engagement Activity | 2018 |
| Description | Keynote lecture - 17th EuroFed Lipid Congress in Seville |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Invited lecture: New insights in the composition of wax and sterol esters in seed oils revealed by ESI-MS/MS |
| Year(s) Of Engagement Activity | 2019 |
| URL | http://www.eurofedlipid.org/pages/sevilla |
| Description | Keynote speaker at Future Foods symposium |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Johnathan Napier was Keynote Speaker at Future Foods symposium, Koln (5-7 September 2018) |
| Year(s) Of Engagement Activity | 2018 |
| Description | Lecture to undergraduate students |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Undergraduate students |
| Results and Impact | A lecture on this project and related research and its impact in general terms was presented to 2nd year undergraduate students specialising in Plant Sciences module from Oxford Brookes University. |
| Year(s) Of Engagement Activity | 2019 |
| Description | Olga Sayanova invited speaker, Conference on Algal Biomass and Bioproducts |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Industry/Business |
| Results and Impact | Olga Sayanova was an invited speaker at Conference on Algal Biomass and Bioproducts, Seattle, USA |
| Year(s) Of Engagement Activity | 2018 |
| Description | Omega-3s from transgenic plants and its role in aquaculture |
| Form Of Engagement Activity | A broadcast e.g. TV/radio/film/podcast (other than news/press) |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Public/other audiences |
| Results and Impact | . |
| Year(s) Of Engagement Activity | 2019 |
| Description | Plant Lipids session at EuroFed Lipid Annual Congress, Belfast |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Johnathan Napier Organized and chaired Plant Lipids session at EuroFed Lipid Annual Congress, Belfast (16-19 September 2018) |
| Year(s) Of Engagement Activity | 2018 |
| Description | Plenary lecture at the GIFS "Emerging Technologies for Global Food Security" Conference |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Johnathan Napier gave a plenary lecture at the GIFS "Emerging Technologies for Global Food Security" Conference in Saskatoon |
| Year(s) Of Engagement Activity | 2018 |
| Description | Presentation at 3rd International Conference on Plant Synthetic Biology, Bioengineering, and Biotechnology |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Peter Eastmond gave a presentation titled "Engineering the Stereoisomeric Structure of Plant Triacylglycerol to Mimic Human Milk Fat" |
| Year(s) Of Engagement Activity | 2019 |
| Description | Presentation at 9th European Symposium on Plant Lipids |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Peter Eastmond gave a presentation titled "Rewriting Triacylglycerol Biosynthesis to Tailor Fatty Acyl Composition" |
| Year(s) Of Engagement Activity | 2016,2019 |
| Description | Presentation at ABBA conference, Seattle |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Presented results of our work on production of phytase in microalgae |
| Year(s) Of Engagement Activity | 2018 |
| Description | Presentation at ELM |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | The identification and tissue-specific localisation of Long-Chain Omega-3 Fatty Acid-Containing Triacylglycerols in Camelina sativa Embryos using MSI. 10th to 12th April, European Lipidomics Meeting, Toulouse, France. |
| Year(s) Of Engagement Activity | 2019 |
| Description | Presentation at ELM |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | High resolution accurate mass (HR/AM) lipidomic approaches to compare the incorporation of EPA and DHA from transgenic Camelina or fish oil into the blood lipids of healthy humans. European Plant Lipid Meeting, 7th - 10th July, Marseille, France |
| Year(s) Of Engagement Activity | 2018,2019 |
| Description | Presentation at ISPL, Japan, 2018 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Presented outputs from research on EPA biosynthesis in diatoms |
| Year(s) Of Engagement Activity | 2018 |
| Description | Presentation at international Conference: Exploiting tomato Andean landraces to improve heat stress |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Presentation at an international meeting on plant physiology covering the work of the collaboration. Exploiting phenotypic plasticity from tomato Andean landraces to improve heat stress tolerance in the face of global warming de Haro LA, De Luca MB, Bianchetti R, Bermudez L, Cernadas A, Lira BS, Michaelson LV, Haslam RP, Rossi M, Carrari F. 2018. Fisiologia Vegetal Cordoba Argentina. 11 - 15th Nov. |
| Year(s) Of Engagement Activity | 2018 |
| URL | http://www.rafv-clafv2018.org/en |
| Description | Presentation at international Conference: Phytochrome-dependent temperature perception modulates isoprenoid metabolism in tomato. |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Presentation at an international meeting on plant genetics covering the work of the collaboration. Phytochrome-dependent temperature perception modulates isoprenoid metabolism in tomato. Bianchetti R, De Luca MB, de Haro LA, Rosado D Bermudez L, Michaelson LV, Haslam RP, Rossi M, Carrari F. 2019. Simposio Brasileiro de Gentetica Molecular de Plantas. 29th Apr 3rd May. Sau Paulo, Brazil. |
| Year(s) Of Engagement Activity | 2019 |
| URL | https://www.sbg.org.br/pt-br/eventos/vii-simposio-brasileiro-de-genetica-molecular-de-plantas/bem-vi... |
| Description | Presentation at international Conference: Phytochromes and temperature dependent perception in Tomato |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Presentation at an international meeting on plant physiology covering the work of the collaboration. Phytochrome-dependent temperature perception modulates isoprenoid metabolism in tomato. Bianchetti R, De Luca MB, de HaroLA, Rosado D, Bermudez L, Michaelson LV, Haslam RP, Rossi M, Carrari F. 2018. Fisiologia Vegetal Cordoba Argentina. 11 - 15th Nov. |
| Year(s) Of Engagement Activity | 2018 |
| URL | http://www.rafv-clafv2018.org/en |
| Description | Presentation at international Conference: Plant Biology Europe |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Presentation at an international meeting on plant physiology covering the work of the collaboration. Epigenetics basis of light-dependent phytochrome perception in tomato fruits |
| Year(s) Of Engagement Activity | 2020 |
| URL | https://europlantbiology2020.org |
| Description | Presentation at international Conference: Temperature and isopreniod metabolism in Tomato |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Presentation at an international meeting on plant physiology covering the work of the collaboration. Temperature modulates lipid and isoprenoids metabolism in tomato fruits through phytochrome-dependent regulation. Bianchetti R, De Luca MB, de Haro LA, Rosado D Bermudez L, Michaelson LV, Haslam RP, Rossi M, Carrari F. 2018. Fisiologia Vegetal Cordoba Argentina. 11 - 15th Nov. |
| Year(s) Of Engagement Activity | 2018 |
| URL | http://www.rafv-clafv2018.org/en |
| Description | Presentation at international Conference: Tomato fruit and leaf lipidomics |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Poster presentation at an international meeting on Lipidomics covering the work of the collaboration. Developing a high-throughput HR/AM method for the analysis of the lipidome in the tissues from tomato. Michaelson LV, de Haro LA, De Luca MB, Bianchetti R, Bermúdez L, Cernadas A, Conte M, Lira BS, Peralta IE, Rossi M, Carrari F, Haslam RP. 2018. 7th European Lipidomics Meeting, 26-29 September Leipzig Germany. |
| Year(s) Of Engagement Activity | 2018 |
| URL | https://home.uni-leipzig.de/elm2018/ |
| Description | Presentation to the parliamentary all-party group on agriculture |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Policymakers/politicians |
| Results and Impact | Johnathan Napier presented to the parliamentary all-party group on agriculture re: regulation of gene editing post-ECJ (10 October 2018) |
| Year(s) Of Engagement Activity | 2018 |
| Description | Rothamsted Research 'Festival of Ideas' Open Weekend |
| Form Of Engagement Activity | Participation in an open day or visit at my research institution |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Public/other audiences |
| Results and Impact | showcasing oilseeds and camelina at Open Weekend |
| Year(s) Of Engagement Activity | 2018 |
| Description | Royal Society Public Dialogue on Genetic Techologies |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Public/other audiences |
| Results and Impact | Public Dialogue re: genetic technologies -. a news briefing about the findings of the public dialogue will take place at the Royal Society on Wednesday 7 March 2018 @ 10:45am. |
| Year(s) Of Engagement Activity | 2018 |
| Description | School Visit (Hitchin, Hertfordshire) |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Schools |
| Results and Impact | Presentation on plant molecular biology and biochemistry to GCSE and A level students form the Hitchin consortium held at Hitchin Girls School, 18/10/2018. Approx. 200 students attended and I spoke to approximately thirty. Many students talked about genetic engineering of plants and one or two mentioned gene editing. There was interest in careers in science including work experience and the Nuffield scheme. |
| Year(s) Of Engagement Activity | 2018 |
| Description | School Visit (Stevenage, Hertfordshire) |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | Presentation on plant molecular biology and biochemistry to GCSE students, 16/11/2018. Approx.60 students attended from Thomas Alleyne School, Stevenage at an event targeted at girls. Students talked about genetic engineering of plants and protecting the environment. There was interest in careers in science including work experience and the Nuffield scheme. The teachers felt it had gone well and that students were more interested in science as a result. |
| Year(s) Of Engagement Activity | 2018 |
| Description | Talk at Annual Stakeholder meeting |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Industry/Business |
| Results and Impact | Annual BRAVO sLoLa stakeholder meetings where members of the breeding/growing industry discuss progress on the BRAVO project. |
| Year(s) Of Engagement Activity | 2018 |
| Description | Talk at Farm Manager conference |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Other audiences |
| Results and Impact | The Director, Dr Achim Dobermann presented the work being carried out in BRAVO, and WP4 to the attendees at the Velcourt farm Manager's Conference on 12th January 2018. |
| Year(s) Of Engagement Activity | 2018 |
| Description | Talk at the 16th Eurofed Lipid Congress - Belfast |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Other audiences |
| Results and Impact | Presented our recently published work: "New insights in the composition of wax and sterol esters in common and mutant sunflower oils revealed by ESI-MS/MS" Demonstration of the impact of this work for oil authentication and detection of fraudulent mixture to an audience of academic and industrial professionals. |
| Year(s) Of Engagement Activity | 2018 |
| URL | https://veranstaltungen.gdch.de/tms/frontend/index.cfm?l=7660&modus= |
| Description | UK Brassica Research Community meeting |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Other audiences |
| Results and Impact | Frederic Beaudoin organised and chaired the UK-Brassica Research Community (UK-BRC) meeting at Rothamsted on 9th May 2018 |
| Year(s) Of Engagement Activity | 2017,2018 |
| Description | UK Brassica Research Community meeting |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Other audiences |
| Results and Impact | Presented outputs from research on yield traits |
| Year(s) Of Engagement Activity | 2019 |
| Description | Undergraduate Lecturing at the University of Nottingham |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Undergraduate students |
| Results and Impact | Biotechnology BSc -Molecular Pharming module Frederic Beaudoin Lecturing plant biotechnology and molecular pharming at the University of Nottingham. Particularly, the metabolic engineering of plant lipid composition for food and non-food industrial applications using research projects carried out at RRes as successful examples. |
| Year(s) Of Engagement Activity | 2016,2017,2018,2019 |
| Description | Undergraduate Lecturing at the University of Sheffield |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Undergraduate students |
| Results and Impact | Freddie Theodoulou gave lectures on flood toelrance in plants and on plant metabolic engineering: from basic research to commercialisation |
| Year(s) Of Engagement Activity | 2019 |
| Description | Workshop Global Bioeconomy Summit |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Johnathan Napier attended the Global Bioeconomy Summit in Berlin and presented at workshop |
| Year(s) Of Engagement Activity | 2018 |
| Description | Workshop presentation at the 30th International Conference on Arabidopsis Research, Wuhan, China |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Hongtao Zhang gave a presentation on tandem timer technology |
| Year(s) Of Engagement Activity | 2019 |
| Description | World AgriTech Panel discussion on gene-editing |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Industry/Business |
| Results and Impact | I chaired the panel discussion on the future applications on gene editing in agriculture at World Agri-Tech Innovation Summit in London, Oct 2018. |
| Year(s) Of Engagement Activity | 2018 |
| URL | https://worldagritechinnovation.com/ |
| Description | panel discussion on potential of gene editing in agriculture at World AgriTecb summit |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Johnathan Napier chaired panel discussion on potential of gene editing in agriculture at World AgriTec B summit, London (16 October 2018) |
| Year(s) Of Engagement Activity | 2018 |
| Description | visit by Stuart Agnew (UKIP MP) |
| Form Of Engagement Activity | Participation in an open day or visit at my research institution |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Policymakers/politicians |
| Results and Impact | 16th Aug 2018 - Johnathan Napier hosted a visit from Stuart Agnew (UKIP MP) |
| Year(s) Of Engagement Activity | 2018 |