Host responses underlying immunity
Lead Research Organisation:
University of Edinburgh
Department Name: The Roslin Institute
Abstract
This project aims to identify host innate and adaptive responses associated with immunity following natural infection or vaccination. Novel strategies will be developed to improve antigen delivery and potentiate vaccine-mediated protection, underpinned by an Immunological Toolbox to study farm animal immunity. Emphasis will be placed on the study of pathogens in their natural farm animal hosts, supported by ongoing collaborations with academia, industry and other Institutes.
Organisations
- University of Edinburgh, United Kingdom (Lead Research Organisation)
- University of Oxford, United Kingdom (Collaboration)
- University of Gothenburg, Sweden (Collaboration)
- Keio University, Japan (Collaboration)
- Indian Council of Agri Research (ICAR), India (Collaboration)
- Ludwig Maximilians University Munich (Collaboration)
- French National Institute of Agricultural Research (Collaboration)
- AB Vista (Collaboration)
- Technical University of Denmark (Collaboration)
- Boehringer Ingelheim, Germany (Collaboration)
- Iowa State University, United States (Collaboration)
- The Pirbright Institute, WOKING (Collaboration)
- GD Animal Health (Collaboration)
- Scotland's Rural College (Collaboration)
- Babraham Institute (Collaboration)
- Heriot-Watt University, United Kingdom (Collaboration)
- Proxima Concepts Limited (Collaboration)
- U.S. Department of Agriculture USDA (Collaboration)
- Semmelweis University (Collaboration)
- International Livestock Research Institute (ILRI) (Collaboration)
- Royal Veterinary College, United Kingdom (Collaboration)
- University of Ghent, Belgium (Collaboration)
- University of Manchester, Manchester, United Kingdom (Collaboration)
Publications
Akram KM
(2018)
An innate defense peptide BPIFA1/SPLUNC1 restricts influenza A virus infection.
in Mucosal immunology
Alfituri OA
(2019)
Effects of host-derived chemokines on the motility and viability of Trypanosoma brucei.
in Parasite immunology
Andersen SH
(2017)
Quantification and phenotypic characterisation of peripheral IFN-? producing leucocytes in chickens vaccinated against Newcastle disease.
in Veterinary immunology and immunopathology
Audzevich T
(2017)
Pre/pro-B cells generate macrophage populations during homeostasis and inflammation
in Proceedings of the National Academy of Sciences
Bradford BM
(2017)
Oral Prion Disease Pathogenesis Is Impeded in the Specific Absence of CXCR5-Expressing Dendritic Cells.
in Journal of virology
Bradford BM
(2018)
Increased susceptibility to oral Trichuris muris infection in the specific absence of CXCR5 CD11c cells.
in Parasite immunology
Cadwell K
(2017)
AvBD1 nucleotide polymorphisms, peptide antimicrobial activities and microbial colonisation of the broiler chicken gut.
in BMC genomics
Chakraborty P
(2017)
Marek's disease virus infection of phagocytes: a de novo in vitro infection model.
in The Journal of general virology
| Description | To understand immune priming and natural immunity, we have used transgenic MacReporter chickens to identify phenotypic and functional differences in myeloid cell populations varying in CSF1R, MHC II and CD11 expression, including distinct macrophage, dendritic cell and M cell-like subpopulations. We have used these birds to study sites and consequences of uptake of model antigens, avian pathogenic E. coli (APEC) in the lung, and Salmonella in the Bursa and intestines. For example, we have examined early interactions between APEC O1 and O2 and myeloid cells in the respiratory tract. Transcriptomic and flow cytometric analysis indicated that 2 cell subpopulations contained bacteria. Most bacteria were found in heterophils and a small percentage in macrophages and dendritic cells. Differences between the APEC serotypes were shown for immune manipulation and avoidance of killing. As another example, in the CSF1R transgenic reporter chickens we previously discovered that the specialised epithelial cells that transcytose antigen at mucosal surfaces, M cells, express the transgene in contrast to mammalian M cells. In collaboration with Proxima Concepts/Vaxcine UK we are investigating targeting these M cells through oral vaccination of chickens with model antigens and recombinant proteins of Eimeria parasites using their vaccine carrier and adjuvant (FTMA, IVVN, Innovate UK funding). In terms of understanding adaptive cell mediated responses to infections and vaccines, we have developed NGS approaches to enable complete characterisation of bovine MHCI and MHCII using the MiSeq platform. We also have used long read sequencing technologies to obtain full length sequences of bovine MHCI. Our local herd at Langhill has now been fully characterised in terms of MHC profiles which provides a unique experimental resource. We also have MHCI typing set up for sheep, goat, buffalo, pig and have provided training to scientists from Kenya, Zambia and Uganda in MHC biology and relevant techniques. We have collected and analysed MHC types from cattle samples from >3000 animals from Pakistan, Zambia, Uganda, Kenya, Brazil, Italy, UK with further sampling in Scotland, Ghana and Nigeria planned for this year. This helps us understand the underlying MHC diversity and how specific disease subtypes and vaccines may be required in different regions of the world. We have collaborated with University of Oxford & the Technical University of Denmark to use MHC peptide elution to define the peptide binding motifs of a variety of bovine MHCI molecules and extrapolated this technology for bovine MHCII molecules. This enables us to identify specific peptides from pathogens that are presented during the infection process to improve vaccine design as well as to inform breeding strategies to select for resistance. Through ISP funding and uplift funding from BMGF, we have generated a pipeline for isolating and characterising bovine antigen-specific B cells. The aim of this work was to enable the analysis of the bovine immunoglobulin response against an immunogen; focussing on invariant surface proteins of trypanosomes as a target. In particular we aimed to identify bovine long-CDR3 domain antibodies, which we hypothesise should be able to bypass the immunodominant and abundant variant surface glycoprotein coat, which sterically hinders access of conventional antibodies to underlying invariant epitopes. We developed an immunisation regime with Trypanosoma brucei invariant surface glycoprotein 65-like (ISG65-like), a successful antigen-specific cell isolation protocol by FACS, involving tetramers of ISG65-like, and have generated methods for isolating and growing bovine B cells for the first time (both through in vitro culture and immortalisation with Theileria annulata). This has resulted in the isolation and characterisation of heavy and light chain paired sequences for 4,500 single antigen-specific B cells. We are currently screening these sequences, and through further uplift funding (BMGF) we aim to express recombinant antibodies and test binding to target antigen - in particular focussing on long-CDR3 domain antibodies. This represents a novel and powerful platform for analysing the bovine adaptive immune response, and identification of antibodies that can bind to invariant surface trypanosome proteins will provide a viable route to pursuing these antibodies as meaningful vaccination targets for trypanosomiasis. In collaboration with ISP1, we have generated transcript and epigenomic atlases (RNAseq, ATACseq & methylation data) for seven bovine immune cell subsets (CD4+, CD8+ & ??T cells, NK cells, B cells, monocytes & neutrophils) from European and African cattle to underpin research on breed-dependent responses to Theileria. In collaboration with Neiker, Spain we investigated the interaction of novel bTB and MAP vaccines with bovine dendritic cells and showed that, at least in part, they induce immune responses through interactions of DC with natural killer cells. We continue to exploit a lymphatic cannulation model in cattle to characterise phenotypically distinct subpopulations of NK cells draining the skin, and have developed assays for interactions between APCs and populations of unconventional T cells or NK cells in the context of M. bovis infection or BCG vaccination. The lymphatic cannulation model is also identifying correlates of protection induced by bTB and Theileria vaccines. We have characterised novel lipopeptides as target epitopes for T-cells and are examining the role of other non-conventional T-cells (MAIT) in bacterial infections in cattle. This is being investigated in collaboration with groups at the University of Oxford and The Pirbirght Institute (BBSRC response mode funded). Building on collaborative research with The Pirbright Institute to define the nature of protective responses induced by vaccines for infectious bronchitis virus in chickens, we identified IBV epitopes that induce antibody responses across virus strains. In collaboration with the University of Oxford, we will predict whether the epitopes are prone to mutate under immune pressure and the bio-informatics pipeline is now applied to predict epitopes of limited variability on the major surface proteins both IBV and AIV (FTMA and IAA funding). ISP staff also supported evaluation of novel vaccines for porcine viruses including testing of a novel prime boosting vaccine concept for influenza A virus comparing a conventional attenuated full virus vaccine and an experimental epitope vaccine. The experimental vaccine enhanced cellular and humoral immunity in vaccinated pigs which, following challenge, also had reduced viral loads in lungs compared to pigs vaccinated just with the commercial vaccine. A virus-vectored multi-component vaccine against porcine reproductive and respiratory syndrome virus, porcine circovirus type 2 and swine influenza is also showing promise. A trivalent vaccine restricts excretion and transmission of Shiga toxin positive E. coli O157 under experimental conditions Research supported by both this Institute Strategic Programme and an FSS/FSA grant, and in collaboration with the Moredun Research Institute and Glasgow University, has demonstrated that our vaccine developed over the last decade to limit enterohaemorrhagic E. coli O157 excretion from cattle is effective when tested using Shiga toxin (Stx) positive E. coli in small group cattle cohorts. This is important as there was some concern that Stx impairs immune responses and while the previously vaccinated animals had been shown to exhibit significantly reduced shedding of a Stx- strain it needed to be tested with a wild type (Stx+) isolate. The experiment also provided evidence that transmission to vaccinated animals was reduced. The data helps us demonstrate the value of testing the vaccine under field conditions for which a commercial partner is still being sought with the aim of licencing. Vaccination of cattle against the protozoan parasite Theileria annulata, using live cultured infected cells, has been practiced for several decades. Successful vaccination is known to be dependent on transfer of the parasite into the cells of recipient animals, to enable generation of protective T cell-mediated immune responses. However, the mechanism by which this occurs has been unclear, since this stage of the parasite does not possess the organelles and proteins required for host cell invasion. Using an in vitro model, we have demonstrated that this parasite transfer occurs as a result of cell fusion, which gives rise to infected genetic hybrid cells capable of inducing the protective immune response. Preliminary findings have implicated certain cell surface proteins in mediating cell fusion. Therefore, successful vaccination against Theileria annulata with cultured infected cells is dependent on the fusogenic capacity of infected cells. We have played a leading role in establishing the International Veterinary Vaccinology Network (IVVN) with the aim of building a community of researchers to address the challenges impeding vaccine development for major livestock and zoonotic diseases in low-and-middle income countries (LMICs). Since launching in August 2017 after receiving £2.8m from the MRC and BBSRC, the IVVN has welcomed over 800 members from more than 60 countries, and importantly 55% of IVVN members are from organisations based in LMICs. In addition to networking activities and catalyst funding, the IVVN aims to be a central area of dissemination of information to the veterinary vaccinology community via the IVVN website. Work linking themes 1 and 3 has detected genetic variation in the response of Boran cattle in Kenya to FMDV vaccination and the underlying basis is under investigation. We have also modelled the epidemiological consequences of different livestock vaccination strategies, using PRRSV in pigs as an exemplar. Toward improved adjuvants (3.3) we have demonstrated that recombinant Flt3 ligand has the potential to target fused antigens to chicken DCs and we have partnered with industry to evaluate novel adjuvant formulations, including cobalt nanoparticles (using M. bovis Ag85 in cattle with Benchmark) and oil-based formulations in chickens for IAV (with Proxima). In collaboration with scientists at Univ. Manchester we have shown that antigen presenting type 3 innate lymphoid cells regulate T cell-dependent IgA responses to commensal and pathogenic bacteria in the colon. Toolbox reagent development: We have generated and validated mAbs specific for ADGRE-1 in pigs, cattle, sheep, humans and rats adding to generation of CSF-1 monoclonals enabling studies of macrophage development and biology across species. We have also generated recombinant bovine CX3CL1 which we have used as a fluorescently labelled probe to determine the expression of CX3CR1 on populations of bovine macrophages and dendritic cells, including those found draining the skin in afferent lymph. We have started to generate an antibody for CD244 and an anti-bovine CD138 antibody. |
| Exploitation Route | This theme of the institute programme is focused on the immune responses of livestock and the primary ambition is to develop vaccine-based interventions as well our understanding of how best these can be applied through modelling studies. We have numerous commercial partners for this research and these partnerships are an important route to 'use by others'. The international veterinary vaccinology network and knowledge exchange with our Centre for Tropical Livestock Genetics & Health (CTLGH) targets impact in low- and middle-income countries (LMICs). We continue to develop new immunological reagents through our 'toolbox' and these are made generally available for use by others in academic and industrial settings. We are developing animal models and expertise in vulnerable skills as well as culture methods to reduce animal use and provide better models of infection and immune responses. |
| Sectors | Agriculture, Food and Drink,Education,Pharmaceuticals and Medical Biotechnology |
| URL | https://www.ed.ac.uk/roslin/research/isp |
| Description | The proposed outputs of this theme of the programme are: correlates of natural immunity and vaccine-mediated protection for vaccine design, potency tests and 3R assays; novel immuno-modulatory substances and delivery systems to potentiate beneficial responses; toolboxes, animal models and expertise for application in veterinary vaccinology; mathematical models of the impact of natural or vaccine-induced immunity on disease prevalence and transmission of zoonoses at population scale; training in vulnerable skills, especially in vivo research in relevant hosts. Specific examples two years into this research include; (1) an association Proxima Concepts/Vaxcine UK to target M-cells during vaccination of poultry; (2) an agreement with Roslin Technologies to trial a trivalent vaccine to restrict E. coli O157 excretion form cattle on a feedlot; (3) Partnership with Neiker (Spain) in development of bTB and MAP vaccines; (4) Partnering with Benchmark to test cobalt nanoparticles as an adjuvant with an bTb subunit vaccine that is in development. The other major area is the generation of livestock species immunological reagents. Under the toolbox section we continue to generate specific reagents, mainly monoclonals, to livestock species surface markers that have value in the commercial and academic sectors. |
| First Year Of Impact | 2017 |
| Sector | Agriculture, Food and Drink,Education,Pharmaceuticals and Medical Biotechnology |
| Impact Types | Economic |
| Description | Member of Defra's Science Advisory Council |
| Geographic Reach | National |
| Policy Influence Type | Participation in a advisory committee |
| Impact | As a member of Defra's Science Advisory Council, I provide comments on a wide variety of Defra science-related policies and draw upon both my personal research experience, but also the broader research community at the Roslin Institute |
| URL | https://www.gov.uk/government/organisations/science-advisory-council/about/our-governance#members |
| Description | BBSRC - BBR call 2017, GCRF highlight call |
| Amount | £75,000 (GBP) |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 07/2017 |
| End | 06/2018 |
| Description | BBSRC Responsive Mode |
| Amount | £644,177 (GBP) |
| Funding ID | BB/R008272/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 04/2018 |
| End | 03/2021 |
| Description | BBSRC-iCASE-Chicken Intestinal Organoids: A Novel In Vitro System To Study Mucosal Vaccine Targeting |
| Amount | £95,000 (GBP) |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 09/2017 |
| End | 09/2021 |
| Description | Bioinformatics and Biological Resources Fund - Andy Law - Facilitating application of NGS to analysis of livestock T-cell receptor repertoires |
| Amount | £71,870 (GBP) |
| Funding ID | BB/P024629/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 07/2017 |
| End | 06/2018 |
| Description | Center for Tropical Livestock Health and Genetics |
| Amount | £15,000,000 (GBP) |
| Organisation | Bill and Melinda Gates Foundation |
| Sector | Charity/Non Profit |
| Country | United States |
| Start | 02/2017 |
| End | 03/2020 |
| Description | Dissecting the molecular pathways of MDV oncoprotein Meq for understanding pathogenesis and aid vaccine development |
| Amount | £312,917 (GBP) |
| Funding ID | BB/R007632/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 06/2018 |
| End | 05/2021 |
| Description | Edinburgh Clinical Academic Track - Consortium funding - Kenny Baillie for RI - ECAT-Plus: The role of CD97 in the host response to infection with influenza A virus |
| Amount | £268,182 (GBP) |
| Funding ID | 203913/Z/16/Z |
| Organisation | Wellcome Trust |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 08/2017 |
| End | 07/2020 |
| Description | GCRF Global Challenges Research Fund - Tim Connelley - International Veterinary Vaccinology Network |
| Amount | £2,113,339 (GBP) |
| Funding ID | MR/R005958/1 |
| Organisation | Medical Research Council (MRC) |
| Sector | Academic/University |
| Country | United Kingdom |
| Start | 07/2017 |
| End | 06/2021 |
| Description | GCRF Impact Accelerator |
| Amount | £300,000 (GBP) |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 09/2017 |
| End | 03/2018 |
| Description | GCRF Impact Accelerator |
| Amount | £300,000 (GBP) |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 09/2017 |
| End | 03/2018 |
| Description | H2020 |
| Amount | € 5,500,000 (EUR) |
| Funding ID | DELTA-FLU |
| Organisation | European Commission H2020 |
| Sector | Public |
| Country | Belgium |
| Start | 05/2017 |
| End | 04/2022 |
| Description | Horizon 2020 - Research Infrastructures - Mark Stevens - REVeterinary biocontained facility network for excellence in animal infectiology research and experimentation |
| Amount | £282,513 (GBP) |
| Funding ID | 731014 |
| Organisation | European Commission |
| Sector | Public |
| Country | European Union (EU) |
| Start | 03/2017 |
| End | 02/2022 |
| Description | Horizon H2020 - Fast Track to Innovation - Lonneke Vervelde - Dynamics of avian influenza in a changing world |
| Amount | £403,373 (GBP) |
| Funding ID | 727922 |
| Organisation | European Commission |
| Sector | Public |
| Country | European Union (EU) |
| Start | 06/2017 |
| End | 05/2022 |
| Description | Horserace betting levy board research call 2017 |
| Amount | £5,800 (GBP) |
| Organisation | Horserace Betting Levy Board |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 05/2018 |
| End | 02/2019 |
| Description | Identification of interferon stimulated genes that restrict cross-species transmission of influenza A virus. |
| Amount | £614,702 (GBP) |
| Funding ID | BB/S00114X/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 10/2018 |
| End | 09/2021 |
| Description | Impact accelerator award |
| Amount | £30,000 (GBP) |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 09/2017 |
| End | 02/2018 |
| Description | International Travel Award Scheme |
| Amount | £2,500 (GBP) |
| Funding ID | BB/R012377/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 11/2017 |
| Description | International Veterinary Vaccinology Network |
| Amount | £600,000 (GBP) |
| Funding ID | MC_PC_17219 |
| Organisation | Medical Research Council (MRC) |
| Sector | Academic/University |
| Country | United Kingdom |
| Start | 03/2018 |
| End | 03/2019 |
| Description | Japan Partnering Award: Defining the factors that regulate M cell-development in the intestines of livestock |
| Amount | £42,125 (GBP) |
| Funding ID | BB/S019294/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 06/2019 |
| End | 06/2023 |
| Description | Newton fund - Christine Tait-Burkard - A strategic approach to identifying and combating porcine reproductive and respiratory syndrome virus outbreaks and other porcine viral diseases |
| Amount | £983,771 (GBP) |
| Funding ID | BB/R013187/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 04/2018 |
| End | 03/2021 |
| Description | Research Grant - Christine Tait-Burkard - Nanobodies as antiviral PRRSV agents |
| Amount | £59,802 (GBP) |
| Funding ID | Roslin Contracts Ref 2191 |
| Organisation | Eco Animal Health Ltd |
| Start | 03/2018 |
| End | 03/2019 |
| Description | Research Grant - Lonneke Vervelde - Development of in vitro chicken enterocyte and organoid cultures |
| Amount | £136,852 (GBP) |
| Funding ID | WT 6571120 |
| Organisation | Novozymes |
| Sector | Public |
| Country | Denmark |
| Start | 12/2018 |
| End | 11/2019 |
| Description | Research Grant - Lonneke Vervelde - Towards edible vaccines for chickens |
| Amount | £99,656 (GBP) |
| Organisation | Medical Research Council (MRC) |
| Sector | Academic/University |
| Country | United Kingdom |
| Start | 03/2019 |
| End | 12/2019 |
| Description | Responsive Mode - Adam Balic - Exploitation of new technologies to advance understanding of avian dendritic cell biology |
| Amount | £589,932 (GBP) |
| Funding ID | BB/R003653/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 11/2017 |
| End | 10/2020 |
| Description | Responsive Mode - Jayne Hope - SAVE: Single-Administration Vaccine Enhancement |
| Amount | £114,896 (GBP) |
| Funding ID | BB/R008272/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 07/2018 |
| End | 06/2021 |
| Description | Responsive Mode - Kellie Watson - The role of interleukin-10 (IL-10) in the regulation of innate immunity in the domestic chicken. |
| Amount | £801,119 (GBP) |
| Funding ID | BB/P022049/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 12/2017 |
| End | 11/2020 |
| Description | Role of distinct mononuclear phagocyte subsets in oral prion disease pathogenesis |
| Amount | £550,023 (GBP) |
| Funding ID | BB/S005471/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 05/2019 |
| End | 04/2022 |
| Description | Sub-award from BBSRC Flexible Talent Mobility Award (BB/R506564/1). Professor Lonneke Vervelde & Dr Sam Ellis. 'Identification of protective epitopes for control of avian influenza virus'. |
| Amount | £14,138 (GBP) |
| Funding ID | BB/R506564/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 11/2017 |
| End | 03/2019 |
| Description | Sub-award from BBSRC Flexible Talent Mobility Award (BB/R506564/1). Professor Lonneke Vervelde, Dr Kate Sutton & Proxima Concepts Ltd. 'Evaluation of a novel adjuvant for mucosal immunistion of poultry'. |
| Amount | £12,830 (GBP) |
| Funding ID | BB/R506564/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 11/2017 |
| End | 03/2019 |
| Description | Sub-award from BBSRC Impact Acceleration Account (BB/S506722/1). Dr Adam Balic 'Dendritic cell targeting to enhance vaccination efficacy in immunologically naïve chicks' |
| Amount | £9,544 (GBP) |
| Funding ID | BB/S506722/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 04/2018 |
| End | 03/2021 |
| Description | Sub-award from BBSRC Impact Accelerator Account (BB/S506722/1). Professor Lonneke Vervelde 'Broad spectrum avian influenza vaccine based on epitopes of low variability' |
| Amount | £20,000 (GBP) |
| Funding ID | BB/S506722/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 04/2018 |
| End | 03/2021 |
| Title | Bovine MHC genotyping using MiSeq |
| Description | A complete platform for enabling the characterising MHC diversity in bovine. It describes the whole process including PCR amplification, sequence analysis and bioinformatics pipeline required to perform high resolution, high throughput analysis of bovine MHC |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2016 |
| Provided To Others? | Yes |
| Impact | Application of this tool is enabling us to perform a global scale analysis of bovine MHC which had previously been technically non-feasible. Based on this technique we are now analysing several thousand samples collected from countries around the world including Pakistan, Kenya, Uganda, Cameroon, Zambia, Brazil, UK and Italy. We have already hosted researchers from Kenya and Zambia who wanted to learn how to use and apply the method. |
| Title | ELISpot for PRRSV |
| Description | An ELISpot for PRRSV was developed to investigate cellular immune responses in vaccine development studies. |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2018 |
| Provided To Others? | No |
| Impact | The tool is essential to do vaccine efficacy studies. |
| Title | In vitro culture of bovine intestinal enteroids |
| Description | We have developed the methodology for generating and culturing bovine intestinal enteroids - stem cell-derived 3-dimensional structures, including a lumen and crypts, and with all cell types represented. Importantly these are able to be continuously passaged, and can be cryopreserved. Therefore, they are (i) a more physiologically relevant in vitro model for the bovine intestine, (ii) represent a viable route to replacing animal use in the study of the bovine intestine - for example, in host-pathogen studies of important bovine enteric pathogens (potentially including drug screening). |
| Type Of Material | Model of mechanisms or symptoms - in vitro |
| Year Produced | 2018 |
| Provided To Others? | Yes |
| Impact | While it is too early to assess impact of our research tool, we have already had multiple enquiries for collaboration and requests for advice on use of the technique, both within Roslin Institute and from external institutions. We predict that this will lead to a reduction in animal use as the use of enteroids becomes more wiodespread. |
| Title | India flu - in vitro assays |
| Description | Development of in vitro assays for assessment of candidate gene function in response to flu infection |
| Type Of Material | Model of mechanisms or symptoms - in vitro |
| Provided To Others? | No |
| Impact | These assays allow us to verify the effects of candidate genes upon flu infection, without using whole animal experiments |
| URL | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4523026/ |
| Title | Avianbase: a community resource for bird genomics |
| Description | The Avianbase Project is an initiative led by the Roslin Institute in collaboration with the Avian Phylogenomics Consortium and Ensembl to make the initial sequence and annotation available for 48 bird species within the Ensembl framework. The Avianbase Project is using the Ensembl infrastructure to share data brought together by the Avian Phylogenomics Consortium (as part of the Avian Genome Consortium) for 48 birds. Giving access to sequence and annotation data for genome assemblies is important because, while facilitating research, it places both assembly and annotation quality under scrutiny, resulting in improvements to both. Therefore we announce Avianbase, a resource for bird genomics, which provides access to data released by the Avian Phylogenomics Consortium. |
| Type Of Material | Database/Collection of data |
| Year Produced | 2015 |
| Provided To Others? | Yes |
| Impact | The Avianbase project was overviewed in the paper of 2015 (Lél Eöry, M Thomas P Gilbert, Cai Li, Bo Li, Alan Archibald, Bronwen L Aken, Guojie Zhang, Erich Jarvis, Paul Flicek and David W Burt (2015) Avianbase: a community resource for bird genomics. Genome Biology 16:21) which has been sited by over 18 times. It provides a valuable one-stop-shop to access a wealth of Avian genomic information to allow easy interrogation of genotype across species. |
| URL | http://avianbase.narf.ac.uk/index.html |
| Title | India flu - chicken transcriptome |
| Description | Transcriptomes from brain, lung and ileum tissue from chicken in response to high and low path avian flu |
| Type Of Material | Database/Collection of data |
| Provided To Others? | No |
| Impact | Identification of genes involved in resistance/susceptibility to avian flu |
| Title | India flu - crow transcriptome |
| Description | Transcriptomes from brain, lung and ileum tissue from crow in response to high and low path avian flu |
| Type Of Material | Database/Collection of data |
| Provided To Others? | No |
| Impact | Identification of genes involved in resistance/susceptibility to avian flu |
| Title | India flu - duck transcriptome |
| Description | Transcriptomes from brain, lung and ileum tissue from duck in response to high and low path avian flu |
| Type Of Material | Database/Collection of data |
| Provided To Others? | No |
| Impact | Identification of genes involved in resistance/susceptibility to avian flu |
| Title | India flu - goose transcriptome |
| Description | Transcriptomes from brain, lung and ileum tissue from goose in response to high and low path avian flu |
| Type Of Material | Database/Collection of data |
| Provided To Others? | No |
| Impact | Identification of genes involved in resistance/susceptibility to avian flu |
| Title | India flu - pigeon transcriptome |
| Description | Transcriptomes from brain, lung and ileum tissue from pigeon in response to high and low path avian flu |
| Type Of Material | Database/Collection of data |
| Provided To Others? | No |
| Impact | Identification of genes involved in resistance/susceptibility to avian flu |
| Title | India flu - turkey transcriptome |
| Description | Transcriptomes from brain, lung and ileum tissue from turkey in response to high and low path avian flu |
| Type Of Material | Database/Collection of data |
| Provided To Others? | No |
| Impact | Identification of genes involved in resistance/susceptibility to avian flu |
| Title | Mathematical model for predicting vaccine effectiveness with accompanied R Shiny app |
| Description | A mathematical model with an interactive R Shiny app was created to model the epidemiological consequences of vaccination with imperfect vaccines of various types, administered using different strategies to animal herds with different replacement rates and heterogeneity in vaccine responsiveness. |
| Type Of Material | Computer model/algorithm |
| Year Produced | 2019 |
| Provided To Others? | Yes |
| Impact | no impact yet |
| URL | https://www.wiki.ed.ac.uk/display/saphir/ |
| Title | Mechanistic model for within host PRRS virus infection dynamics |
| Description | A mechanistic model was developed to assess candidate immune mechanisms responsible for viremia rebound. The model was fitted to viremia data from a large scale PRRS virus infection experiment. The model is published in Go N. et al. 2019. How to prevent viremia rebound: evidence from a PRRSv data-supported model of immune response. BMC Systems Biol. https://doi.org/10.1186/s12918-018-0666-7 |
| Type Of Material | Computer model/algorithm |
| Year Produced | 2019 |
| Provided To Others? | Yes |
| Impact | Now collaborative research grant applications |
| Title | PRJEB14552 |
| Description | The MHC region contains many genes that are key regulators of both innate and adaptive immunity including the polymorphic MHCI and MHCII genes. Consequently, the characterisation of the repertoire of MHC genes is critical to understanding the variation that determines the nature of immune responses. Currently our knowledge of the bovine MHCI repertoire is limited with only the Holstein-Friesian breed having been studied in any depth. Traditional methods of MHCI genotyping are of low resolution and laborious and this has been a major impediment to a more comprehensive analysis of the MHCI repertoire of other cattle breeds. Next-generation sequencing (NGS) technologies have been used to enable high throughput and much higher resolution MHCI typing in a number of species. In this study we have developed a MiSeq platform approach and requisite bioinformatics pipeline to facilitate typing of bovine MHCI repertoires. The method was validated initially on a cohort of Holstein-Friesian animals and then demonstrated to enable characterisation of MHCI repertoires in African cattle breeds, for which there was limited or no available data. During the course of these studies we identified >140 novel classical MHCI genes and defined 62 novel MHCI haplotypes, dramatically expanding the known bovine MHCI repertoire. Construction protocol: Total RNAs were extracted from white blood cell pellets prepared form blood obtained by venepuncture using Tri-reagent (Sigma-Aldrich, Dorset) and cDNA prepared using the Reverse Transcription Kit (Promega, WI); both according to the manufacturer's protocols. PCR amplification was performed with the Phusion HF PCR kit (NEB, Herts.) using bovine MHCI primers that included Illumina sequencing primer annealing sites, multiple sample identifier tags (D501-508 and D701-712) and Illumina ligation adaptors. PCR products from ~96 animals were pooled to form a library which was then sequenced on the Illumina Miseq platform. |
| Type Of Material | Database/Collection of data |
| Year Produced | 2016 |
| Provided To Others? | Yes |
| Impact | These data represent a reference dataset of novel MHC alleles in cattle and may underpin research into cattle immunogenetic diversity for many years in the future |
| URL | https://www.ebi.ac.uk/ena/data/view/PRJEB14552 |
| Title | PRJEB14979 |
| Description | Marek's Disease Virus (MDV) is a widespread alphaherpesvirus of poultry that causes Marek's disease (MD) characterised by fatal visceral CD4+ TCRaß+ T cell tumours at high incidence in susceptible hosts. As is the case with many virus-induced tumours, immortal cell lines harbouring viral genome have been generated from ex vivo cultures of MD tumours. As readily-available sources of large numbers of cells of a uniform type, MDV-transformed lymphoblastoid cell lines (LCL) have proved extremely valuable in studying virus-host interaction. While the viral genome is held in a latent state in most of the cells, a minor population of cells display spontaneous reactivation identifiable by the expression of lytic viral genes such as pp38. The process of spontaneous reactivation in these cell populations offers the opportunity for investigating the biological processes involved in the reactivation events. For this, we used two lymphoblastoid cell lines derived from lymphomas induced by pRB1B-UL47eGFP, a recombinant MDV engineered to express EGFP fused with the UL47. We used Fluorescence-activated cell sorting (FACS) to purify the rare EGFP-positive cell population with spontaneously activating viral genome from the majority EGFP-negative cells and analysed their gene expression profiles by RNA-sequencing using Illumina HiSeq2500. The reads generated were mapped using TopHat and gene expression levels were analysed by edgeR. Ingenuity pathway analysis software on more than 2000 differentially-expressed genes between the lytically infected (EGFP-positive) and latently infected (EGFP-negative) cell populations was used identify major biological pathways involved in the reactivation. These studies revealed that amongst others, transcripts directly involved in T activation such as CD3 complex, CD28, ICOS, phospholipase C, CD3 complex were down-regulated following reactivation of the virus in the LCL. |
| Type Of Material | Database/Collection of data |
| Year Produced | 2017 |
| Provided To Others? | Yes |
| Impact | These data detail the gene expression changes during spontaneous activation of MDV and will be useful to researchers investigating the mechanism of action of this important virus, as well as in vaccine design |
| URL | https://www.ebi.ac.uk/ena/data/view/PRJEB14979 |
| Title | mRNA-seq analysis data derived from in vitro bovine ileum enteroid cultures |
| Description | In vitro 3D enteroid, or "mini gut", cultures were prepared from bovine small intestinal (ileal) crypts. mRNA was isolated from bovine small intestinal crypts, freshly prepared enteroids (P0) and enteroids after multiple serial passages (P1-5). Global gene expression was compared in these samples by mRNA-seq. |
| Type Of Material | Database/Collection of data |
| Year Produced | 2018 |
| Provided To Others? | Yes |
| Impact | Significant interest from many other researchers was generated from these data |
| URL | https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE112674 |
| Description | Centre for Tropical Livestock Genetics and Health (CTLGH) |
| Organisation | International Livestock Research Institute (ILRI) |
| Country | Kenya |
| Sector | Charity/Non Profit |
| PI Contribution | Research contribution to the CTLGH result framework. |
| Collaborator Contribution | Research links and collaboration with its member partners in the UK and Africa. |
| Impact | No output to report yet. |
| Start Year | 2017 |
| Description | Centre for tropical livestock genetics and health (CTLGH) |
| Organisation | International Livestock Research Institute (ILRI) |
| Country | Kenya |
| Sector | Charity/Non Profit |
| PI Contribution | Mick Watson is co-leading programme 5 (informatics and bioresources) programme of CTLGH which aims to collect data on genotype and phenotype into a central database which can subsequently be mined for useful associations. So far we have ensured that scientists have access to the latest high performance computing environment for research, we have analysed and continue to analyse hundreds of farm animal genomes from LMIC countries, and we have built the data portal (http://data.ctlgh.org) |
| Collaborator Contribution | The partners are involved in all programmes, which include:Program 1: Harnessing genetic variability among indigenous and exotic breeds of cattle (as well as their crosses) to develop genetic and genomic tools that will be used to improve productivity under harsh tropical conditions and to mitigate the impact of cattle on climate change. Program 2: Harnessing genetic variability in tropical productivity and adaptation among various breeds of Chickens. Program 3: Development and application of precision breeding (through novel reproductive and germplasm technologies) to achieve step changes in livestock genetic improvement. Program 4: Understanding the genetic and molecular mechanisms underlying the tolerance of certain cattle and poultry breeds to tropical diseases and pests. Program 5: A shared global data and biological sample resource to support continued research and development on tropical livestock genetics and health. |
| Impact | The data portal so far: http://data.ctlgh.org The collaboration is multi-disciplinary, bringing together geneticists, parasitologists, virologists, epidemiologists and data scientists together. |
| Start Year | 2016 |
| Description | Centre for tropical livestock genetics and health (CTLGH) |
| Organisation | Scotland's Rural College |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Mick Watson is co-leading programme 5 (informatics and bioresources) programme of CTLGH which aims to collect data on genotype and phenotype into a central database which can subsequently be mined for useful associations. So far we have ensured that scientists have access to the latest high performance computing environment for research, we have analysed and continue to analyse hundreds of farm animal genomes from LMIC countries, and we have built the data portal (http://data.ctlgh.org) |
| Collaborator Contribution | The partners are involved in all programmes, which include:Program 1: Harnessing genetic variability among indigenous and exotic breeds of cattle (as well as their crosses) to develop genetic and genomic tools that will be used to improve productivity under harsh tropical conditions and to mitigate the impact of cattle on climate change. Program 2: Harnessing genetic variability in tropical productivity and adaptation among various breeds of Chickens. Program 3: Development and application of precision breeding (through novel reproductive and germplasm technologies) to achieve step changes in livestock genetic improvement. Program 4: Understanding the genetic and molecular mechanisms underlying the tolerance of certain cattle and poultry breeds to tropical diseases and pests. Program 5: A shared global data and biological sample resource to support continued research and development on tropical livestock genetics and health. |
| Impact | The data portal so far: http://data.ctlgh.org The collaboration is multi-disciplinary, bringing together geneticists, parasitologists, virologists, epidemiologists and data scientists together. |
| Start Year | 2016 |
| Description | Collaboration with AB Vista |
| Organisation | AB Vista |
| Country | United Kingdom |
| Sector | Private |
| PI Contribution | We are researching the activity of ovodefensins, naturally-occurring egg peptides with antimicrobial properties. With BBSRC LINK funding we are exploring the activity of diverse ovodefensins from multiple avian species, dissecting their structure and mode of action, and exploring their capacity to act as growth promoters and novel therapeutics. |
| Collaborator Contribution | AB Vista have made a substantial cash contribution to the LINK project (c. £460k) and also provide access to natural populations of broilers for feed trials using diets supplemented (or not) with Trichoderma-expressed ovodefensins. They also support analysis of the impact of such diets on intestinal microbiota and metabolites. |
| Impact | Commercially sensitive and to be disclosed following scrutiny for Intellectual Property. |
| Start Year | 2012 |
| Description | Collaboration with Boehringer Ingelheim |
| Organisation | Boehringer Ingelheim |
| Country | Germany |
| Sector | Private |
| PI Contribution | The collaboration is to advance diagnostics in relation to specific bacterial infections in pigs. We are carrying out basic science investigating antigens and immune responses |
| Collaborator Contribution | The company is providing reagents and expertise in this subject area |
| Impact | Part-funded PhD studentship |
| Start Year | 2017 |
| Description | Collaborator for grant BB/M003094/1 |
| Organisation | Semmelweiss University |
| Department | Faculty of Medicine |
| Country | Hungary |
| Sector | Academic/University |
| PI Contribution | Exchange of biological samples and reagents |
| Collaborator Contribution | Exchange of biological samples and reagents |
| Impact | exchange of antibodies to detect avian cellular markers |
| Start Year | 2015 |
| Description | Collaborators on grant BB/M012069 |
| Organisation | The Pirbright Institute |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Performed joined animal experiment related to the grant objective 1. |
| Collaborator Contribution | Performed joined animal experiment related to the grant objective 1. |
| Impact | No output yet, project started recently. |
| Start Year | 2015 |
| Description | Collaborators on grant BB/M028208 |
| Organisation | U.S. Department of Agriculture USDA |
| Department | Beltsville Agricultural Research Center |
| Country | United States |
| Sector | Academic/University |
| PI Contribution | We have performed a pilot study to investigate the susceptibility of different chicken lines to APEC. |
| Collaborator Contribution | They have performed a large scale APEC trial that informed us on how to set up the pilot trial. |
| Impact | This project only recently started, no outcomes yet. |
| Start Year | 2015 |
| Description | Collaborators on grant BB/M028305 |
| Organisation | French National Institute of Agricultural Research |
| Country | France |
| Sector | Public |
| PI Contribution | Performed a pilot study to investigate susceptibility of chicken lines to APEC. |
| Collaborator Contribution | Analysed APEC virulence |
| Impact | No outputs yet, project recently started. |
| Start Year | 2015 |
| Description | Collaborators on grant BB/M028305 |
| Organisation | Ludwig Maximilian University of Munich (LMU Munich) |
| Country | Germany |
| Sector | Academic/University |
| PI Contribution | Performed pilot study to investigate susceptibility of chicken lines to APEC. |
| Collaborator Contribution | Developed histological tools. |
| Impact | No outputs yet, project started recently. |
| Start Year | 2015 |
| Description | Designing new vaccine delivery systems for cattle |
| Organisation | Heriot-Watt University |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | This collaboration between Jayne Hope and Vicki Stone/Ferry Melchels at Heriot Watt University aims to develop implantable devices that will be engineered to release vaccine components under the skin of cattle at defined time points. This will enable prime-boost vaccination to be delivered in a single shot. The team at Heriot-Watt provide expertise in development of the device including materials expertise and 3D printing, whereas the team at Roslin provide in vitro and in vivo expertise to assess vaccine release, and immunogenicity. |
| Collaborator Contribution | This collaboration between Jayne Hope and Vicki Stone/Ferry Melchels at Heriot Watt University aims to develop implantable devices that will be engineered to release vaccine components under the skin of cattle at defined time points. This will enable prime-boost vaccination to be delivered in a single shot. The team at Heriot-Watt provide expertise in development of the device including materials expertise and 3D printing, whereas the team at Roslin provide in vitro and in vivo expertise to assess vaccine release, and immunogenicity. |
| Impact | This collaboration has led to a BBSRC grant funded in 2018 (BB/R008272/1). We appointed the first member of staff in September 2018 and a second member of staff will join the team in July 2019. |
| Start Year | 2017 |
| Description | GD animal health Deventer, collaborators on grant BB/M01206911 |
| Organisation | GD Animal Health |
| Country | Netherlands |
| Sector | Private |
| PI Contribution | GD Animal Health has performed VN assays for our project |
| Collaborator Contribution | provided us with antisera, protocols and virus |
| Impact | imroved performance of |
| Start Year | 2015 |
| Description | India DBT collaboration |
| Organisation | Indian Council of Agricultural Research |
| Department | National Institute of High Security Animal Diseases |
| Country | India |
| Sector | Public |
| PI Contribution | Using the tissues collected in Bhopal, we have sequenced and analysed the avian transcriptomic response to avian influenza challenge in 6 bird species (chicken, turkey, crow, goose, pigeon, duck). |
| Collaborator Contribution | All influenza challenge experiments were carried out by the team in Bhopal, tissues collected and sent over to the UK for processing and analysis |
| Impact | Identification of candidate genes for resistance to avian flu. |
| Start Year | 2014 |
| Description | Oxford University, Evolutionary ecology of infectious diseases |
| Organisation | University of Oxford |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Collaboration on broad spectrum vaccines in which we provide the veterinary input (materials). Postdoc in my group obtain BBSRC impact accelerator award. |
| Collaborator Contribution | Collaboration on broad spectrum vaccines in which they provide bio-informatics pipeline and screening technology. |
| Impact | BBSRC IAA |
| Start Year | 2017 |
| Description | PRRS Host Genetics Consortium |
| Organisation | Iowa State University |
| Country | United States |
| Sector | Academic/University |
| PI Contribution | Genetic analysis of infectious disease data and mathematical modelling of PRRS infection dynamics; Contribution to experimental design of PRRS challenge and field experiments and sampling; |
| Collaborator Contribution | Access to data; access to statistical models; scientific advice for model assumptions |
| Impact | Joint manuscripts and publications: 1. Go N., Islam Z., Lunney J., Belloc C., Touzeau S.& Doeschl-Wilson A.B., 2017. Neutralizing antibodies prevent PRRS viremia rebound: evidence from a data-supported model of the immune response. PloS Comp. Biol. Under review 2. Lough G., Hess A., Dekkers JCM, Hess M., Kyriazakis I., Mulder H., Lunney J, Rowland BB, Doeschl-Wilson A.B. 2017. Harnessing longitudinal information to identify genetic variation in tolerance of pigs to Porcine Reproductive and Respiratory Syndrome virus. Genetics Selection Evolution. Under review 3. Lough G., Rashidi H., Kyriazakis I., Dekkers JCM, Hess AS, Hess MK, Deeb N, Kause A., Lunney J, Rowland BB, Mulder H and Doeschl-Wilson A.B. 2017. Use of multi-trait and random regression models to identify genetic variation in tolerance to Porcine Reproductive and Respiratory Syndrome virus. Genetics Selection Evolution, 49(1)37. 3. 4. Hess, A.S., Islam, Z., Hess, M.K., Rowland, R.R., Lunney, J.K., Doeschl-Wilson, A., Plastow, G.S. and Dekkers, J.C., 2016. Comparison of host genetic factors influencing pig response to infection with two North American isolates of porcine reproductive and respiratory syndrome virus. Genetics Selection Evolution, 48(1), p.1.18. 5. Islam Z.U., Bishop S.C., Savill N.J. , Rowland R.R.R., Lunney J.K., Trible B and Doeschl-Wilson A.B. 2013. Quantitative analysis of Porcine Reproductive and Respiratory Syndrome (PRRS) viremia profiles from experimental infection: a statistical modelling approach. PLoS One, DOI: 10.1371/journal.pone.0083567. ( |
| Start Year | 2012 |
| Description | Proxima |
| Organisation | Proxima Concepts Limited |
| PI Contribution | Generated preliminary data to apply for funding which was successful. |
| Collaborator Contribution | Supplied material to test in in vivo experiments to apply for funding. |
| Impact | Obtained multiple joined small and large research grants |
| Start Year | 2018 |
| Description | RVC |
| Organisation | Royal Veterinary College (RVC) |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | We generated preliminary data for funding applications, next step will be to use their parasites in the awarded projects. |
| Collaborator Contribution | Supply of material and large scale animal trials. |
| Impact | Applied for joint grants |
| Start Year | 2014 |
| Description | Role of M cells in the sampling of mucosal antigens by B cells in Peyer's patches |
| Organisation | University of Gothenburg |
| Department | Microbiology and Immunology UGOT |
| Country | Sweden |
| Sector | Academic/University |
| PI Contribution | We provided our expertise in M cell immunobiology and use of unique in vivo systems to study M cells |
| Collaborator Contribution | Prof. Nils Lycke is an expert in mucosal immunology and provided his unique systems in which to study the generation of antigen-specific IgA responses in the intestine. |
| Impact | A revised manuscript has been submitted to Nature Communications (January 2019) describing how activated Peyer´s patch B cells sample antigen directly from M cells in the subepithelial dome to maintain gut germinal center responses |
| Start Year | 2018 |
| Description | Saphir - Horizon2020 |
| Organisation | French National Institute of Agricultural Research |
| Country | France |
| Sector | Public |
| PI Contribution | I am leader of workpackage 16: Modelling vaccine efficacy |
| Collaborator Contribution | INRA is project leader |
| Impact | -Scientific Publication: Go N., Islam Z., Lunney J., Belloc C., Touzeau S.& Doeschl-Wilson A.B., 2019. How to prevent viremia rebound: evidence from a PRRSv data-supported model of immune response. BMC Systems Biol. https://doi.org/10.1186/s12918-018-0666-7 -Popular article "How to prevent viremia rebound in virus infections? Evidence from a data-driven mathematical model for PRRS". Doeschl-Wilson A., Go N., Touzeau S. SAPHIR newsletter Nov 2017 - Issue 4. Project coordinator statement "A very innovative approach for modelling the immune response has been designed and validated." -Interactive Wiki-website: Vaccine effectiveness in the field. https://www.wiki.ed.ac.uk/display/saphir/ -Conference talks: International Vaccinology Meeting in Edinburgh, May 2018; Annual Meeting for the Society for Mathematical Biology, Sydney Australia, July 2018 and the Conference for Research Workers in Animal Disease, Chicago December 2018 |
| Start Year | 2016 |
| Description | Saphir - Horizon2020 |
| Organisation | University of Ghent |
| Department | Faculty of Veterinary Medicine |
| Country | Belgium |
| Sector | Academic/University |
| PI Contribution | I am leader of workpackage 16: Modelling vaccine efficacy |
| Collaborator Contribution | INRA is project leader |
| Impact | -Scientific Publication: Go N., Islam Z., Lunney J., Belloc C., Touzeau S.& Doeschl-Wilson A.B., 2019. How to prevent viremia rebound: evidence from a PRRSv data-supported model of immune response. BMC Systems Biol. https://doi.org/10.1186/s12918-018-0666-7 -Popular article "How to prevent viremia rebound in virus infections? Evidence from a data-driven mathematical model for PRRS". Doeschl-Wilson A., Go N., Touzeau S. SAPHIR newsletter Nov 2017 - Issue 4. Project coordinator statement "A very innovative approach for modelling the immune response has been designed and validated." -Interactive Wiki-website: Vaccine effectiveness in the field. https://www.wiki.ed.ac.uk/display/saphir/ -Conference talks: International Vaccinology Meeting in Edinburgh, May 2018; Annual Meeting for the Society for Mathematical Biology, Sydney Australia, July 2018 and the Conference for Research Workers in Animal Disease, Chicago December 2018 |
| Start Year | 2016 |
| Description | Study of role of genes and transcription factors in M-cell development |
| Organisation | Keio University |
| Country | Japan |
| Sector | Academic/University |
| PI Contribution | This collaboration aims to determine the role of genes and transcription factors in M-cell development in large animal species. Prof. Mabbott obtained a BBSRC travel award in 2017 to enable him to visit the Japanese partner Prof. Koji Hase in Tokyo, Japan, to discuss a potential collaboration. Prof. Mabbott through obtaining the travel award initiated this collaboration and provided the means to explore potential collaborations. |
| Collaborator Contribution | Prof. Koji Hase, the Japanese partner, has provided his extensive expertise in the role of genes and transcription factors in M cell development. During my visit his institution also provided in-kind support for our meetings and small workshop. Since this visit our discussions have continued and a BBSRC Japan partnering award was submitted in November 2018 to provide further pump priming support. |
| Impact | A BBSRC Japan partnering award was submitted in November 2018 |
| Start Year | 2017 |
| Description | Study of the role of Cxcr5 in ectopic germinal centre formation in the lung |
| Organisation | Babraham Institute |
| Department | Lymphocyte Signalling |
| Country | United Kingdom |
| Sector | Charity/Non Profit |
| PI Contribution | Our major contribution was the provision of our unique Cxcr5-floxed mice to this project. These mice were originally created on BBSRC grant BB/F019726/1. |
| Collaborator Contribution | Our lead partner, Dr. Michelle Linterman at the Babraham Institute used these mice to study the role of Cxcr5 in ectopic germinal centre formation in the lung. |
| Impact | Paper published in the Journal of Experimental Medicine in 2019 "Type I interferon induces CXCL13 to support ectopic germinal center formation" JEM 03-04-2019 issue, vol. 216 no. 3. http://doi.org/10.1084/jem.20181216 |
| Start Year | 2016 |
| Description | Study role of CXCR5 expression in ILC3 cells |
| Organisation | University of Manchester |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | We provided the unique CXCR5-floxed mice created in this project to Dr. Matthew Hepworth at the University of Manchester. |
| Collaborator Contribution | The CXCR5-floxed mice were crossed to ID2 ER2 Cre - ROSA-RFP mice to enable the study of effects of CXCR5-deficiency in ILC3 cells. |
| Impact | Manuscript published in the Journal of Experimental Medicine in February 2019. PMID:30814299 "Antigen presenting ILC3 regulate T cell-dependent IgA responses to colonic mucosal bacteria" by Felipe Melo-Gonzalez, Hana Kammoun, Elza Evren, Emma Dutton, Markella Papadopoulou, Barry Bradford, Ceylan Tanes, Fahmina Fardus-Reid, Jonathan Swann, Kyle Bittinger, Neil Mabbott, Bruce Vallance, Tim Willinger, David Withers, and Matthew Hepworth |
| Start Year | 2017 |
| Description | Use of MHC elution to define functional parameters of bovine MHC haplotypes |
| Organisation | Technical University of Denmark |
| Country | Denmark |
| Sector | Academic/University |
| PI Contribution | We provide the biological context for the studies, prepare laboratory materials for analysis and subsequent in vitro validation of the data generated by our collaborators |
| Collaborator Contribution | The Jenner Institute perform the tandem nPLC-mass spectrometry and preliminary peptide identification. The Technical University of Denmark performs subsequent bioinformatics analysis. |
| Impact | Publication - PMID: 29115832. Novel information on the peptide-binding motifs of bovine MHCI molecules. Preliminary data for granny submission. Collaboration to support a post-graduate Brazilian student to undertake a 9 month FAPESP-funded scholarship at the collaborating laboratories. |
| Start Year | 2015 |
| Description | Use of MHC elution to define functional parameters of bovine MHC haplotypes |
| Organisation | University of Oxford |
| Department | Jenner Institute |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | We provide the biological context for the studies, prepare laboratory materials for analysis and subsequent in vitro validation of the data generated by our collaborators |
| Collaborator Contribution | The Jenner Institute perform the tandem nPLC-mass spectrometry and preliminary peptide identification. The Technical University of Denmark performs subsequent bioinformatics analysis. |
| Impact | Publication - PMID: 29115832. Novel information on the peptide-binding motifs of bovine MHCI molecules. Preliminary data for granny submission. Collaboration to support a post-graduate Brazilian student to undertake a 9 month FAPESP-funded scholarship at the collaborating laboratories. |
| Start Year | 2015 |
| Description | 'Science Insights' "Host responses underlying immunity" |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | Developed and run through EBSOC at Easter Bush Campus, Science Insights is on-going work experience programme designed to give 5th year high school pupils a real insight into the work and life of research scientists. Science taster sessions delivered under "Host responses underlying immunity" included "The Use of flow cytometry to characterise macrophage subsets in mouse tissue" "Mouse model of inflammatory bowel disease" "Parasite burden in alapacas - faecal worm counts" |
| Year(s) Of Engagement Activity | 2018 |
| URL | https://www.ed.ac.uk/medicine-vet-medicine/outreach/science-insights |
| Description | Activity at Roslin Open Day |
| Form Of Engagement Activity | Participation in an open day or visit at my research institution |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Public/other audiences |
| Results and Impact | Heather Mathie in my research group designed an activity relating to the immune response and vaccines. This was to explain the value of vaccines for animals, and the studies we are undertaking to understand what comprises a protective immune response and how we might design new vaccines using this information. |
| Year(s) Of Engagement Activity | 2018 |
| Description | Annual Roslin Institute Open Doors Day |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Public/other audiences |
| Results and Impact | The annual Roslin Institute Open Doors day was held on 14 October 2017 and was attended by 491 people. It involved 81 members of staff and students and showcased a wide range of research arising from BBSRC strategic investment, including (but not limited to), food safety, influenza, genetic improvement, DNA, imaging and the ethical treatment of animals in research. |
| Year(s) Of Engagement Activity | 2017 |
| Description | Annual Roslin Institute Open Doors Day |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Public/other audiences |
| Results and Impact | The annual Roslin Institute Open Doors day was held on 14 October 2017 and was attended by 491 people. It involved 81 members of staff and students and showcased a wide range of research arising from BBSRC strategic investment, including (but not limited to), food safety, influenza, genetic improvement, DNA, imaging and the ethical treatment of animals in research. |
| Year(s) Of Engagement Activity | 2017 |
| Description | Avian Pathogenesis Meeting, Shanghai Veterinary Research Institute. "Generation of genome wide CRISPR Cas9 libraries for pig and chicken" |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | The Avian Pathogenesis Meeting was organised to provide an opportunity for interaction and collaboration with veterinary scientists from China. I presented our work on genome-wide CRISPR/cas9 libraries for pig and chicken and ISG libraries for pig and chicken. We highlighted our new capability to perform high throughput systematic functional screens in livestock species. Potential collaborations and sharing of resources were discussed and are currently being acted on. |
| Year(s) Of Engagement Activity | 2018 |
| Description | BBC Countryfile film related to the work of the Centre for Tropical Livestock Genetics & Health at Roslin |
| Form Of Engagement Activity | A broadcast e.g. TV/radio/film/podcast (other than news/press) |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Public/other audiences |
| Results and Impact | Bill Gates was interviewed by BBC Countryfile on 26 January 2018 in relation to work funded by the Gates Foundation involving Roslin Institute scientists towards the genetic improvement of farmed animals in low- and middle-income countries. The programme aired on 11 February 2018 and reached an estimated audience in excess of 5 million. The interview took place on the same day as the launch of the Global Academy of Agriculture & Food Security, which was attended by the Secretary of State for the Department for International Development the Rt Hon Penny Mordaunt (see separate activity) and generated significant press interest, featuring on BBC Scotland news. Particular emphasis was placed on the work of Roslin scientists to understand the genetic make-up of African diary cattle and chickens and to understand how this relates to performance traits in order to guide breeding decisions. |
| Year(s) Of Engagement Activity | 2018 |
| Description | BBSRC-DBT Livestock Health and Disease workshop (2012), Delhi, India in Feb 2012 |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | BBSRC-DBT Livestock Health and Disease workshop (2012), Delhi, India in Feb 2012 no actual impacts realised to date |
| Year(s) Of Engagement Activity | 2012 |
| Description | British society for Immunology |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Postgraduate students |
| Results and Impact | Poster presentation at the British Society for Immunology, Dec 2017. Poster entitled: Precision cut lung slices: A platform for examining host-pathogen interactions in an avian model. Authors on the posters: Bryson K, Esposito M., Lamont S., Stevens M., McLaughlan G. and Vervelde L. |
| Year(s) Of Engagement Activity | 2017 |
| Description | Campus Open Day- Midlothian Science Festival |
| Form Of Engagement Activity | Participation in an open day or visit at my research institution |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Public/other audiences |
| Results and Impact | The Roslin Institute and Easter Bush Campus Open Day was an event undertaken within the Midlothian Science Festivals. Hands on public activities lead by scientists under "Host responses underlying immunity" included Science is for Everyone |
| Year(s) Of Engagement Activity | 2018 |
| URL | https://www.ed.ac.uk/easter-bush-campus/science-outreach-centre |
| Description | Conference talk on PRRS infection dynamics |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | A. Wilson presented the talk "Is PRRSV viremia rebound preventable? Evidence from a data-supported mechanistic model of the immune response" at the Conference for research workers in Animal Diseases (CRWAD) in Chicago 2018. The talk sparked intense discussions about the occurrence and role of viremia rebound in infectious diseases. |
| Year(s) Of Engagement Activity | 2018 |
| URL | https://crwad.org/2018-crwad-conference/ |
| Description | EBSOC Led Engagement Activities- Talks/Discussions |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Public/other audiences |
| Results and Impact | The Easter Bush Science Outreach Centre (EBSOC) which opened in January 2018 is the first of its kind in Scotland. EBSOC is a unique, purpose-built laboratory space where visitors, including primary and secondary pupils and their teachers, engage with real-life science. Supported by scientists from "Host responses underlying immunity", EBSOC's schools workshops enable pupils to meet scientists from The Roslin Institute. As well as supporting scientist-lead workshops both to National and International audiences, scientists undertaking research within "Host responses underlying immunity" undertake discussions and talks within these activities on research undertaken within this theme the control of diseases between humans and animals. |
| Year(s) Of Engagement Activity | 2018 |
| URL | https://www.ed.ac.uk/easter-bush-campus/science-outreach-centre |
| Description | Edinburgh India Institute, Inaugural conference on 15-16 May 2014 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Policymakers/politicians |
| Results and Impact | Gave a short talk about avian influenza BBSRC-DBT project and how it came about, joint talk with our India collaborators. Edinburgh India Institute, Inaugural conference on 15-16 May 2014. John McIntyre Conference Centre, Edinburgh. Conference theme: Innovative Engagement for Sustainable Development: the Edinburgh-India Story. |
| Year(s) Of Engagement Activity | 2014 |
| Description | Flu talk - journalists |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Media (as a channel to the public) |
| Results and Impact | Presentation of our work on avian flu to a selection of Indian and Japanese journalists. Government representatives were also present. Interest in future Scottish/Indian proposals was shown. |
| Year(s) Of Engagement Activity | 2015 |
| Description | Host and convenor of the joint SAPHIR and VET-VAC workshop, Roslin, UK, May 2018 |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Postgraduate students |
| Results and Impact | The Roslin Institute hosted the 2nd International Veterinary Vaccinology workshop with the goal to provide scientists and practicioners integrated and up-to-date knowledge on the challenges facing the development of effective veterinary vaccines |
| Year(s) Of Engagement Activity | 2018 |
| URL | http://www.vetvaccnet.ac.uk/events/european-veterinary-vaccinology-workshop-2018 |
| Description | ISAG talk |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Postgraduate students |
| Results and Impact | Talk title: Mapping QTLs affecting Marek's disease by selective DNA pooling in eight lines across 15 generations |
| Year(s) Of Engagement Activity | 2017 |
| Description | Institute of Animal Pathogology, University of Bern. "Bringing high throughput approaches to livestock species". Invited talk. |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Was invited to Bern to present on our work on developing CRISPR/Cas9 and ISG libraries for pig and chicken. Collaborations and sharing of resources were discussed and are being put into action. Additionally the visit was organised to increase links with an institute with aligned scientific interests. |
| Year(s) Of Engagement Activity | 2018 |
| Description | International PRRSV symposium - South Korea |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | 60-100 practitioners met to learn about advances in PRRSV including control and diagnostics. |
| Year(s) Of Engagement Activity | 2018 |
| Description | Introduction to the Research of the Roslin Institute - Royal Society of Biology Scottish Branch Annual Symposium |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Professional Practitioners |
| Results and Impact | Professor Stevens provided an overview of the research of The Roslin Institute to members of the public and the Royal Society of Biology, who convened for the annual symposium of the RSB Scottish Branch on 14 October 2017 at Roslin. The event coincided with the annual Roslin 'Open Doors' day and featured BBSRC-funded research. |
| Year(s) Of Engagement Activity | 2017 |
| Description | Invited speaker - Avian Genetics and Immunity Symposium |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Research on Avian Genetics and Immunity (RAGI) has been established to strengthen global partnerships and links between academic researchers, students and the poultry professionals working on the genetics of avian immunity. The purpose of the symposium was to provide a forum for discussion and exchange of ideas around the single theme of exploiting available opportunities in ultimately dealing emerging and existing infectious avian diseases in poultry production systems and to safeguard food supplies and human health. The purpose of KW talk was to overview the current situation in industry for breeding for disease resistance and introduce the NARF. The overview of NARF included a review of facilities, lines available and current work being carried out using NARF avian lines. The presentation stimulated significant discussion. |
| Year(s) Of Engagement Activity | 2017 |
| URL | http://geneticsandimmunity.com/ |
| Description | Invited speaker at the In vitro host-pathogen workshop 2018, Moredun Institute |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | Invited talk at the In vitro host-pathogen workshop 2018, Moredun Institute. Talk focused on 3Rs solutions to study host pathogen interaction in poultry. |
| Year(s) Of Engagement Activity | 2018 |
| Description | Invited talk European Veterinary Vaccinology Workshop 2018 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Postgraduate students |
| Results and Impact | Talk on career opportunities in academia for young scientists |
| Year(s) Of Engagement Activity | 2018 |
| URL | https://www.youtube.com/watch?v=hTujbImUCcI |
| Description | Invited talk at symposium |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Industry/Business |
| Results and Impact | North American PRRS symposium. Chicago, USA, Dec 2017. Novel insights about host response to PRRS from data driven mathematical models. Invited talk. |
| Year(s) Of Engagement Activity | 2017 |
| Description | Munster flu meeting |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | 5th international influenza meeting. IFITM talk. Munster, Germany. Sep 2016 |
| Year(s) Of Engagement Activity | 2016 |
| Description | Open lecture on African trypanosomes in cattle to Royal Society of Biology Scottish Branch |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Professional Practitioners |
| Results and Impact | Dr Liam provided an overview of his research on African trypanosomes in cattle to members of the public and the Royal Society of Biology, who convened for the annual symposium of the RSB Scottish Branch on 14 October 2017 at Roslin. The event coincided with the annual Roslin 'Open Doors' day and featured BBSRC-funded research. |
| Year(s) Of Engagement Activity | 2017 |
| Description | Organisation of Workshop on "Genome engineering and wider applications" at the WVPA 2017 |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Organised a preconference workshop on "Genome engineering and wider applications" for the World Veterinary Poultry Association with talks on "Editing techniques and biobanking", "The transgenic hen bioreactor", "Ethics and consumer acceptance, and "Use of transgenic chickens in disease and vaccine studies". Number of WVPA participants was ~2300. |
| Year(s) Of Engagement Activity | 2017 |
| URL | http://www.wvpa.net/congress.php |
| Description | PAG 2018 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Invited speaker at Plant and Animal Genome conference 2018 (San Diego, USA) 'Avian flu: the winners and the losers' - Jan 2018 |
| Year(s) Of Engagement Activity | 2018 |
| Description | Poultry Breeders Roundtable |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | • Invited speaker at National poultry breeders roundtable meeting (St Louis, USA) - JS May 2017 |
| Year(s) Of Engagement Activity | 2017 |
| Description | Presentation to Edinburgh Farm Animal Vet Society |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Undergraduate students |
| Results and Impact | I was invited to give a presentation on my research to a group of undergraduate students with an interest in bovine diseases. This was to highlight the importance of research in influencing the future of veterinary vaccines and diagnostics, as well as to enthuse them and encourage the students to consider scientific research careers. The talk was well attended, the students asked lots of good questions and some were planning to look for placements within labs for the summer research component of their course. |
| Year(s) Of Engagement Activity | 2018 |
| Description | Press release on publication describing effects of ageing on human immune system |
| Form Of Engagement Activity | A press release, press conference or response to a media enquiry/interview |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Media (as a channel to the public) |
| Results and Impact | A press statement was released describing our study published in the Journal of Allergy and Clinical Immunology on the effects of a drug, losmapimod, in improving immunity in the skin of elderly humans. |
| Year(s) Of Engagement Activity | 2018 |
| URL | https://www.ed.ac.uk/roslin/news-events/latest-news/improving-vaccines-elderly-inflammation |
| Description | Press releases JV IBV vaccine |
| Form Of Engagement Activity | A press release, press conference or response to a media enquiry/interview |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Press release was written after our publication in Journal of Virology on novel IBV vaccines. The press release was picked up and published by multiple magazines in the veterinary and agriculture field including: front page with photo; Vet Times - https://www.vettimes.co.uk/news/vaccine-shows-promise-against-chicken-disease/ Farming UK - https://www.farminguk.com/news/New-vaccine-could-help-fight-against-major-poultry-disease_50541.html Agriland - https://www.agriland.co.uk/farming-news/vaccine-shows-promise-against-costly-poultry-disease/ Stackyard - http://www.stackyard.com/news/2018/10/vet/06_edinburgh_vaccine.html Global Meat News - https://www.globalmeatnews.com/Article/2018/10/23/Chicken-disease-vaccine-progress Technology Networks - https://www.technologynetworks.com/tn/news/vaccine-shows-promise-against-chicken-disease-310951 Farming Sector - https://www.farmingsector.co.uk/latest-news/livestock/new-vaccine-could-protect-poultry-from-serious-disease/ Medical Xpress- https://medicalxpress.com/news/2018-10-vaccine-widespread-chicken-disease.html News Medical - https://www.news-medical.net/news/20181023/New-vaccine-strategy-shows-promise-to-protect-chickens-against-serious-respiratory-disease.aspx Feed stuff - https://www.feedstuffs.com/nutrition-health/vaccine-shows-promise-against-ibv Science Daily - https://www.sciencedaily.com/releases/2018/10/181023110540.htm Genetic Literacy Project - https://geneticliteracyproject.org/2018/10/25/bronchitis-in-chickens-can-cut-egg-production-70-new-vaccine-may-stem-outbreaks/ |
| Year(s) Of Engagement Activity | 2018 |
| Description | Press reports on PRRSV-resistant pigs |
| Form Of Engagement Activity | A press release, press conference or response to a media enquiry/interview |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Public/other audiences |
| Results and Impact | In connection with the publication of our research paper "Pigs Lacking the Scavenger Receptor Cysteine-Rich Domain 5 of CD163 Are Resistant to Porcine Reproductive and Respiratory Syndrome Virus 1 Infection" (doi: 10.1128/JVI.00415-18) we published a press package and answered interview request from many broadcasting and media agencies. As a result the following print and broadcast pieces were released: International Broadcast: BBC Scotland, BBC News at Six, BBC News at Ten, BBC World, BBC Sunday Morning Live, BBC Radio 4 Farming Today, BBC Radio Five Live, BBC Radio 4, BBC Radio Scotland, BBC World Service, Guardian Podcast, BBC Radio Shetland, Swiss Radio & TV (SRF), Country Today (Victoria, Australia, Radio), Fiji Broadcasting Corporation, France 24 Print UK: The Daily Telegraph, The Times, The Guardian, Scottish Daily Mail, The i, Scottish Daily Express, The Sun, Scottish Sun, Daily Mirror, Daily Star, Metro, The Herald, Scotsman, Daily Record, Dundee Courier, Edinburgh Evening News, Aberdeen Evening Express, The Week, Press & Journal, Yorkshire Post Online UK BBC Online, Daily Telegraph, Daily Mail, The Times, The Guardian, Daily Mirror, Daily Express, Scotsman, Irish News, Irish Examiner, Shropshire Star, Express & Star, Hastings Observer, Scarborough News, Eastbourne Herald, Shoreham Herald, West Sussex County Times, Yorkshire Evening Post, Glasgow South & Eastwood Extra, Rye & Battle Observer, Crawley Observer, Bognor Regis Observer, Mid Sussex Times, Worthing Herald, Littlehampton Gazette, Bexhill-on-Sea Observer, Portsmouth News, Chichester Observer, Midhurst & Petworth Observer, Dundee Evening Telegraph, Aberdeen Evening Express, Independent Recorder, TDNews, BT.com, BreakingNews.ie, The London Economic, The Weekly Observer, The Scientist, The London Economic, Science Magazine, Earth.Com, Independent Recorder, BT.com, Breakingnews.IE, I4U News, Press & Journal, Livekindly.co, Tech Times, IFLScience, Technology Networks Online / Print International: NTN24 (CST Science, Health & Technology Magazine, Latin Americas), Infosurhoy, The Economic Times (India), First Post (India), Eurasia Review, HealthEuropa, Jstor Daily, Times Of India, St Lucia News Online, Times Now (India), Business Standard (India), CanIndia.com (India), Green Report (Italy), New Kerala.com, Fanpage.It (Italy), Le Monde Veterinaire (France) Veterinary / Science-related press: Agri-Pulse, Veterinary Times, Vet Record, Pig World, Feedstuffs, Laboratory Equipment, GlobalMeatNews.com, Animal Pharm, Ag Daily, Agribusiness Intelligence, Laboratory Equipment, Pig Progress, Pig World, Labiotech.EU, MRCVS, Farmweek, National Hog Farmer, Farming UK, Frontline Genomics, Farmers Weekly, Food Weekly News |
| Year(s) Of Engagement Activity | 2018,2019 |
| URL | https://www.ed.ac.uk/roslin/news-events/latest-news/gene-edited-pigs-resistant-billion-dollar-virus |
| Description | Public lecture - Inaugural Lecture of Professor Tanja Opriessnig |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Schools |
| Results and Impact | Professor Opriessnig delivered her Inaugual Lecture on 'From 'circus virus' to mostly commonly used swine vaccine on 24 April 2017, relating to her BBSRC-funded research on porcine viral diseases and strategies to detect and control them. The event was attended by children from local schools, members of the public and students of the University of Edinburgh. |
| Year(s) Of Engagement Activity | 2017 |
| Description | Public lecture entitled Confronting the Microbial Menace in Our Food'. Professor Mark Stevens |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Schools |
| Results and Impact | The inaugural lecture of Professor Mark Stevens was held on 30 October 2017 on his BBSRC-funded research to identify bacterial and host factors influencing the ability of Salmonella, Campylobacter and E. coli to colonise farm animals and cause disease. It was attended by children from local schools, members of the public, students at The Roslin Institute and wider University of Edinburgh and posted online. |
| Year(s) Of Engagement Activity | 2017,2018 |
| URL | https://media.ed.ac.uk/media/Inaugural+lectureA+Confronting+the+microbial+menace+in+our+food/1_x5k5e... |
| Description | Roslin flu meeting |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | 'Avian flu: the winners and the losers' at Annual influenza meeting, Roslin Nov 2017 |
| Year(s) Of Engagement Activity | 2017 |
| Description | School Workshop- DNA Profiling: The Great Escape |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Schools |
| Results and Impact | This workshop was developed and run at the Easter Bush Science Outreach Centre where pupils aged 13-18 took part in this hands-on, researcher-supported workshop linking the techniques of DNA extraction and gel electrophoresis to research on animal behaviour. Participants gained an insight into real-life research and a chance to meet scientists as part of the session and reported an increased interest in science after the session. |
| Year(s) Of Engagement Activity | 2018,2019 |
| URL | https://www.ed.ac.uk/easter-bush-campus/science-outreach-centre |
| Description | School Workshop- ELISA- Flu Fighters |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Schools |
| Results and Impact | This workshop was run three times at the Easter Bush Science Outreach Centre to pupils studying Advanced Higher Biology attended this hands-on, researcher-supported workshop, which is based around an ELISA as well as other laboratory techniques from the curriculum, with a research-linked storyline about flu infection in wild birds and chickens. Pupils and teachers reported an improved understanding of the techniques used, after taking part in the workshop as well as an increased interest in immunology and infectious diseases after meeting researchers working in these areas. |
| Year(s) Of Engagement Activity | 2018 |
| URL | https://www.ed.ac.uk/easter-bush-campus/science-outreach-centre |
| Description | Science Insights Programme for S5 school pupils |
| Form Of Engagement Activity | Participation in an open day or visit at my research institution |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | We ran our Science Insights work experience programme for local school pupils on 24-28 July 2017. 38 S5 pupils attended, 20 of whom worked in the Roslin Institute for 2 days alongside BBSRC-supported researchers from this ISP, including our core-funded staff scientists. All 38 students also had a tour of the Langhill farm and attended a presentation and debate about the use of animals in research. Students also had the opportunity to discuss career paths and what it is like to work as a research scientist. Specifically Jayne Hope and Carly Hamilton presented a short talk and activity related to our work on Cryptosporidium parvum infection in calves. |
| Year(s) Of Engagement Activity | 2017 |
| Description | Science Insights work experience for S5 school pupils |
| Form Of Engagement Activity | Participation in an open day or visit at my research institution |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | We ran our Science Insights work experience programme for local school pupils on 24-28 July 2017. 38 S5 pupils attended, 20 of whom worked in the Roslin Institute for 2 days alongside BBSRC-supported researchers from this ISP, including our core-funded staff scientists. All 38 students also had a tour of the Langhill farm and attended a presentation and debate about the use of animals in research. Students also had the opportunity to discuss career paths and what it is like to work as a research scientist. |
| Year(s) Of Engagement Activity | 2017 |
| URL | https://www.ed.ac.uk/roslin/working/opportunities/young-people/science-insights |
| Description | Stand at the Royal Highland Show, including the BBSRC-funded Flu Fighters exhibit. |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Public/other audiences |
| Results and Impact | Roslin Institute stand at the Royal Highland Show 22-25 June 2017. Approximately 25 Roslin Institute staff and students were involved. Exhibits of relevance to this ISP include the BBSRC-funded Flu Fighters exhibit, highlighting the problem of influenza and our research to combat it. |
| Year(s) Of Engagement Activity | 2017 |
| Description | Summer course |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Postgraduate students |
| Results and Impact | Armidale Animal Breeding Summer Course 2018: Mathematical modeling of infection dynamics in genetically diverse livestock populations Teachers: Andrea Doeschl-Wilson and Osvaldo Anacleto Dates: Monday 5 February 2018 (9am) - Thursday 8 February 2018 (4pm) Venue: University of New England, Armidale, NSW Australia; ~40 post-graduate students, academics and industrial researcher attended the workshop in modelling. Based on the positive course evaluation, the course sparked new research in the important field of mathematical modelling of infection dynamics and genetics |
| Year(s) Of Engagement Activity | 2018 |
| URL | https://jvanderw.une.edu.au/aabc2018.htm |
| Description | Swine Practitioner workshop - China |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Professional Practitioners |
| Results and Impact | Practitioners met to learn about advances in PCV2 research including control and diagnostics. |
| Year(s) Of Engagement Activity | 2018 |
| Description | Swine Practitioner workshop - France |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Industry/Business |
| Results and Impact | Key opinion leaders from Asia were invited to discuss advances in PCV2 research including control and diagnostics. |
| Year(s) Of Engagement Activity | 2018 |
| Description | TEDx Talk by Professor Helen Sang on 'Why do we need GM chickens?' |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Public/other audiences |
| Results and Impact | Professor Helen Sang delivered a Tedx Talk in Glasgow on 2 June 2017 on 'Why do we need GM chickens?'. The event was attended by approximately 1700 people and has been shared online with over 900 views. It highlighted the challenge of achieving global food security and the potential of BBSRC-funded research to improve poultry, including via genetic alteration. Examples for ongoing research using GM poultry in this programme were shared. |
| Year(s) Of Engagement Activity | 2017 |
| URL | https://www.youtube.com/watch?v=DUbqrh5otWs |
| Description | Talk to undergraduate veterinary students as part of Wellcome funded INSPIRE scheme |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Undergraduate students |
| Results and Impact | A short talk was given on my career, choice of research path in order to enthuse undergraduate veterinary sciences students and encourage them to consider a career in science. This was part of a Wellcome Trust funded initiative called INSPIRE. This was well attended, and the students asked a lot of questions related to careers for vets that could combine science as well as veterinary medicine. |
| Year(s) Of Engagement Activity | 2017 |
| Description | The Human Body activities at a BBC-sponsored Edinburgh Festival event |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | Researchers from The Roslin Institute joined colleagues from across the University's College of Medicine and Veterinary Medicine in the grounds of George Heriot's School in central Edinburgh to deliver family-friendly activities about 'The Human Body' on 15 August 2017. The event was part of a series of science-themed activities hosted by the BBC at the Edinburgh Festivals to support BBC Learning's 'Terrific Scientific' campaign, which aims to inspire children and young people to engage with science. Our staff and Ph.D students ran interactive activities about their research on lungs and skin, including inflating and examining real pig lungs, comparing healthy and diseased organs, and a chance for visitors to use their craft skills to make a model of their skin. Other activities at the event included anatomy face and body painting and a chance to see dissections of animal brains and hearts close-up. An estimated 1,400 family visitors were engaged, with great questions about our research and what it's like to work as a scientist. |
| Year(s) Of Engagement Activity | 2017 |
| URL | https://www.ed.ac.uk/roslin/community-engagement/public-events/events-archive/the-human-body-bbc-at-... |
| Description | VGTI, Portland Oregon. ""Discovering novel host-pathogen interactions using systematic approaches" |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Was invited to give a presentation at Portland VGTI on our work on Human cytomegalovirus. |
| Year(s) Of Engagement Activity | 2018 |
| Description | Visit by Bill Gates and the Secretary of State for the Department of International Development |
| Form Of Engagement Activity | A press release, press conference or response to a media enquiry/interview |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Public/other audiences |
| Results and Impact | Bill Gates and the Secretary of State for the Department of International Development (DfID; Rt Hon Penny Mordaunt) visited The Roslin Institute on 26 January 2018. This involved separate sessions with the Gates Foundation and DfID to learn about the research undertaken by the Centre for Tropical Livestock Genetics & Health (CTLGH), in which the Roslin Institute is a major partner and researchers in the Institute Strategic Programmes are heavily involved. DfID announced £4m of funding for the CTLGH, adding to £10m already received from the Gates Foundation. In the presence of the Principal of the University of Edinburgh, Bill Gates and the Rt Hon Penny Mordaunt formally opened the Global Academy for Agriculture & Food Security which will provide undergraduate and postgraduate training in sustainable agriculture. The event took place in the new Campus Innovation Building (which received BBSRC strategic funding). The University of Edinburgh press office tracked the following activity related to the visit, in which many participants in the ISPs were involved: • 109 individual articles and broadcast pieces that mentioned the University with regards to the visit, with an estimated potential reach of more than 7 million people. • Of these, more than half of the coverage mentioned one of more of the Global Academy of Agriculture & Food Security, the Centre for Tropical Livestock Genetics & Health, SEBI, the Royal (Dick) School of Veterinary Studies, Roslin or the Easter Bush Campus in addition to name checking the University. • In addition to the UK, we also attracted press coverage in the USA, France, New Zealand, China, Zimbabwe, South Africa, Botswana, Taiwan and Egypt. • Across social media, we reached more than 1.4 million people with 14k+ engagements (likes, shares, retweets etc). Of these, 99.8% were positive. • The Facebook Live streaming alone reached more than 100k people. More than 18k people have watched the video. • Our news videos have garnered more than 35k views across platforms. • We tracked a spike in traffic to the Global Academy website on the day of the visit - 1405 visits (11 times the average for the previous month). |
| Year(s) Of Engagement Activity | 2018 |
| Description | WGALP talk |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Postgraduate students |
| Results and Impact | Talk entitled 'QTLs mapped by selective DNA pooling explain substantial proportion of phenotypic and genetic variation in MD mortality in chicken layer lines' presented at WGALP in Auckland, Feb 2018 |
| Year(s) Of Engagement Activity | 2018 |
| Description | Wiki-website for vaccine effectiveness in the field |
| Form Of Engagement Activity | Engagement focused website, blog or social media channel |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | A wiki-website was developed at the University of Edinburgh wiki-server under the link: https://www.wiki.ed.ac.uk/display/saphir/ as an outcome of the H2020 project Saphir. The wiki-website outlines risk factors that compromise vaccine effectiveness in the field, with a specific emphasis on PRRS. The website includes a user friendly modelling app based on a predictive model created as part of the Saphir project that predicts the impact of vaccination, that will help a wide range or people interested in vaccine effectivenes in the field to extract conclusions regarding the factors that put in risk the infection invasion in a herd and the factors that mitigate disease spread and pathogen evolution in farm animal populations. |
| Year(s) Of Engagement Activity | 2019 |
| URL | https://www.wiki.ed.ac.uk/display/saphir/ |
| Description | article around a vaccine showing promise against widespread chicken disease |
| Form Of Engagement Activity | A press release, press conference or response to a media enquiry/interview |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Industry/Business |
| Results and Impact | article and press release around a vaccine showing promise against widespread chicken disease. Appeared on Roslin Institute webpages plus in various industry publications and medical and veterinary publications plus local press |
| Year(s) Of Engagement Activity | 2018 |
| Description | dissecting the interferon response to human cytomegalovirus |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Professional Practitioners |
| Results and Impact | Invited seminar to the Edinburgh infectious disease symposium. |
| Year(s) Of Engagement Activity | 2018 |