Host responses underlying immunity
Lead Research Organisation:
University of Edinburgh
Department Name: The Roslin Institute
Abstract
This project aims to identify host innate and adaptive responses associated with immunity following natural infection or vaccination. Novel strategies will be developed to improve antigen delivery and potentiate vaccine-mediated protection, underpinned by an Immunological Toolbox to study farm animal immunity. Emphasis will be placed on the study of pathogens in their natural farm animal hosts, supported by ongoing collaborations with academia, industry and other Institutes.
Organisations
- University of Edinburgh, United Kingdom (Lead Research Organisation)
- University of Oxford, United Kingdom (Collaboration)
- Iowa State University, United States (Collaboration)
- Scotland's Rural College (Collaboration)
- Indian Council of Agri Research (ICAR), India (Collaboration)
- International Livestock Research Institute (ILRI) (Collaboration)
- AB Vista (Collaboration)
- Technical University of Denmark (Collaboration)
- Boehringer Ingelheim, Germany (Collaboration)
Publications
Akram KM
(2018)
An innate defense peptide BPIFA1/SPLUNC1 restricts influenza A virus infection.
in Mucosal immunology
Alfituri OA
(2019)
Effects of host-derived chemokines on the motility and viability of Trypanosoma brucei.
in Parasite immunology
Andersen SH
(2017)
Quantification and phenotypic characterisation of peripheral IFN-? producing leucocytes in chickens vaccinated against Newcastle disease.
in Veterinary immunology and immunopathology
Audzevich T
(2017)
Pre/pro-B cells generate macrophage populations during homeostasis and inflammation.
in Proceedings of the National Academy of Sciences of the United States of America
Bradford BM
(2017)
Oral Prion Disease Pathogenesis Is Impeded in the Specific Absence of CXCR5-Expressing Dendritic Cells.
in Journal of virology
Bradford BM
(2018)
Increased susceptibility to oral Trichuris muris infection in the specific absence of CXCR5 CD11c cells.
in Parasite immunology
Cadwell K
(2017)
AvBD1 nucleotide polymorphisms, peptide antimicrobial activities and microbial colonisation of the broiler chicken gut.
in BMC genomics
Chakraborty P
(2017)
Marek's disease virus infection of phagocytes: a de novo in vitro infection model.
in The Journal of general virology
| Description | 1. International Veterinary Vaccinology Network The aim of the International Veterinary Vaccinology Network (IVVN) is to establish a community of researchers that will address the challenges impeding vaccine development for major livestock and zoonotic diseases in low-and-middle income countries (LMICs). Since launching in August 2017 after receiving £2.8m from the MRC and BBSRC, the IVVN has welcomed 275 members from across 40 countries, and importantly 55% of IVVN members are from organisations based in LMICs. To facilitate collaborations between scientists, industrial partners and others, the IVVN will host annual scientific meetings, and IVVN members will have the opportunity to host workshops. The first meeting of the IVVN is on the 26th & 27th March 2018 in the Ole Sereni Hotel, Nairobi. IVVN members are eligible to apply for catalyst funding in the form of 'pump-priming' grants and laboratory exchange awards. Pump-priming grants, of up to £100,000, are available to allow collaborative teams of IVVN members to address key bottlenecks affecting the development of livestock vaccines. In addition to networking activities and catalyst funding, the IVVN aims to be a central area of dissemination of information to the veterinary vaccinology community via the IVVN website. 2. Development of new immunological reagents We have generated and validated mAbs specific for porcine ADGRE-1 and CSF-1 enabling studies of macrophage development from bone marrow progenitors (Waddell et al, 2 papers in preparation). We have also generated recombinant bovine CX3CL1 which we have used as a fluorescently labelled probe to determine the expression of CX3CR1 on populations of bovine macrophages and dendritic cells, including those found draining the skin in afferent lymph (Raper et al, manuscript in preparation). 3. Development of novel adjuvants With respect to identification of novel adjuvants and potentiating immune response induction, we have formed a new collaboration with Professor Jim Brewer, University of Glasgow and a commercial partner (Aptamer Group) to assess the potential of DNA aptamers as adjuvants for cattle. Preliminary data generated using ISP funding has demonstrated that within DNA aptamer libraries there are aptamers that bind bovine dendritic cells (DC) and appear to potentiate the interactions of DC with T cells indicating they have adjuvant activity. 4. A trivalent vaccine restricts excretion and transmission of Shiga toxin positive E. coli O157 under experimental conditions Research supported by both this Institute Strategic Programme and an FSS/FSA grant, and in collaboration with the Moredun Research Institute and Glasgow University, has demonstrated that our vaccine developed over the last decade to limit enterohaemorrhagic E. coli O157 excretion from cattle is effective when tested using Shiga toxin (Stx) positive E. coli in small group cattle cohorts (unpublished). This is important as there was some concern that Stx impairs immune responses and while the previously vaccinated animals had been shown to exhibit significantly reduced shedding of a Stx- strain it needed to be tested with a wild type (Stx+) isolate. The experiment also provided evidence that transmission to vaccinated animals was reduced. The data helps us demonstrate the value of testing the vaccine under field conditions for which a commercial partner is still being sought with the aim of licencing. 5. Testing of a chimeric PRRS vaccines In collaboration with Virginia Polytechnic Institute and State University, we have collaborated on the testing of a chimeric porcine reproductive and respiratory syndrome virus containing the full-length sequences of shuffled structural genes of multiple heterologous strains. It was shown that the chimeric virus induced a high level of neutralizing antibodies in pigs against two heterologous strains. A subsequent vaccination and challenge study in pigs revealed that the chimeric virus conferred an enhanced cross-protection when challenged with heterologous virus strain or a contemporary heterologous strain RFLP 1-7-4. The results suggest that the chimera may be a good PRRSV vaccine candidate for further development to confer heterologous protection. A virus-vectored multi-component vaccine against porcine reproductive and respiratory syndrome virus, porcine circovirus type 2 and swine influenza is also showing promise. |
| Exploitation Route | xx |
| Sectors | Agriculture, Food and Drink,Education |
| Description | BBSRC - BBR call 2017, GCRF highlight call |
| Amount | £75,000 (GBP) |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 07/2017 |
| End | 06/2018 |
| Description | BBSRC Responsive Mode |
| Amount | £644,177 (GBP) |
| Funding ID | BB/R008272/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 04/2018 |
| End | 03/2021 |
| Description | BBSRC-iCASE-Chicken Intestinal Organoids: A Novel In Vitro System To Study Mucosal Vaccine Targeting |
| Amount | £95,000 (GBP) |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 09/2017 |
| End | 09/2021 |
| Description | CTLGH research project call 2016 |
| Amount | £98,000 (GBP) |
| Organisation | Bill and Melinda Gates Foundation |
| Sector | Charity/Non Profit |
| Country | United States |
| Start | 09/2016 |
| End | 08/2019 |
| Description | Center for Tropical Livestock Health and Genetics |
| Amount | £15,000,000 (GBP) |
| Organisation | Bill and Melinda Gates Foundation |
| Sector | Charity/Non Profit |
| Country | United States |
| Start | 02/2017 |
| End | 03/2020 |
| Description | GCRF Impact Accelerator |
| Amount | £300,000 (GBP) |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 09/2017 |
| End | 03/2018 |
| Description | GCRF Impact Accelerator |
| Amount | £300,000 (GBP) |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 09/2017 |
| End | 03/2018 |
| Description | Horserace betting levy board research call 2017 |
| Amount | £5,800 (GBP) |
| Organisation | Horserace Betting Levy Board |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 05/2018 |
| End | 02/2019 |
| Description | Impact accelerator award |
| Amount | £30,000 (GBP) |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 09/2017 |
| End | 02/2018 |
| Description | he role of interleukin-10 (IL-10) in the regulation of innate immunity in the domestic chicken. |
| Amount | £1,144,442 (GBP) |
| Funding ID | BB/P022049/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 12/2017 |
| End | 12/2020 |
| Title | Bovine MHC genotyping using MiSeq |
| Description | A complete platform for enabling the characterising MHC diversity in bovine. It describes the whole process including PCR amplification, sequence analysis and bioinformatics pipeline required to perform high resolution, high throughput analysis of bovine MHC |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2016 |
| Provided To Others? | Yes |
| Impact | Application of this tool is enabling us to perform a global scale analysis of bovine MHC which had previously been technically non-feasible. Based on this technique we are now analysing several thousand samples collected from countries around the world including Pakistan, Kenya, Uganda, Cameroon, Zambia, Brazil, UK and Italy. We have already hosted researchers from Kenya and Zambia who wanted to learn how to use and apply the method. |
| Title | India flu - in vitro assays |
| Description | Development of in vitro assays for assessment of candidate gene function in response to flu infection |
| Type Of Material | Model of mechanisms or symptoms - in vitro |
| Provided To Others? | No |
| Impact | These assays allow us to verify the effects of candidate genes upon flu infection, without using whole animal experiments |
| URL | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4523026/ |
| Title | Avianbase: a community resource for bird genomics |
| Description | The Avianbase Project is an initiative led by the Roslin Institute in collaboration with the Avian Phylogenomics Consortium and Ensembl to make the initial sequence and annotation available for 48 bird species within the Ensembl framework. The Avianbase Project is using the Ensembl infrastructure to share data brought together by the Avian Phylogenomics Consortium (as part of the Avian Genome Consortium) for 48 birds. Giving access to sequence and annotation data for genome assemblies is important because, while facilitating research, it places both assembly and annotation quality under scrutiny, resulting in improvements to both. Therefore we announce Avianbase, a resource for bird genomics, which provides access to data released by the Avian Phylogenomics Consortium. |
| Type Of Material | Database/Collection of data |
| Year Produced | 2015 |
| Provided To Others? | Yes |
| Impact | The Avianbase project was overviewed in the paper of 2015 (Lél Eöry, M Thomas P Gilbert, Cai Li, Bo Li, Alan Archibald, Bronwen L Aken, Guojie Zhang, Erich Jarvis, Paul Flicek and David W Burt (2015) Avianbase: a community resource for bird genomics. Genome Biology 16:21) which has been sited by over 18 times. It provides a valuable one-stop-shop to access a wealth of Avian genomic information to allow easy interrogation of genotype across species. |
| URL | http://avianbase.narf.ac.uk/index.html |
| Title | India flu - chicken transcriptome |
| Description | Transcriptomes from brain, lung and ileum tissue from chicken in response to high and low path avian flu |
| Type Of Material | Database/Collection of data |
| Provided To Others? | No |
| Impact | Identification of genes involved in resistance/susceptibility to avian flu |
| Title | India flu - crow transcriptome |
| Description | Transcriptomes from brain, lung and ileum tissue from crow in response to high and low path avian flu |
| Type Of Material | Database/Collection of data |
| Provided To Others? | No |
| Impact | Identification of genes involved in resistance/susceptibility to avian flu |
| Title | India flu - duck transcriptome |
| Description | Transcriptomes from brain, lung and ileum tissue from duck in response to high and low path avian flu |
| Type Of Material | Database/Collection of data |
| Provided To Others? | No |
| Impact | Identification of genes involved in resistance/susceptibility to avian flu |
| Title | India flu - goose transcriptome |
| Description | Transcriptomes from brain, lung and ileum tissue from goose in response to high and low path avian flu |
| Type Of Material | Database/Collection of data |
| Provided To Others? | No |
| Impact | Identification of genes involved in resistance/susceptibility to avian flu |
| Title | India flu - pigeon transcriptome |
| Description | Transcriptomes from brain, lung and ileum tissue from pigeon in response to high and low path avian flu |
| Type Of Material | Database/Collection of data |
| Provided To Others? | No |
| Impact | Identification of genes involved in resistance/susceptibility to avian flu |
| Title | India flu - turkey transcriptome |
| Description | Transcriptomes from brain, lung and ileum tissue from turkey in response to high and low path avian flu |
| Type Of Material | Database/Collection of data |
| Provided To Others? | No |
| Impact | Identification of genes involved in resistance/susceptibility to avian flu |
| Title | PRJEB14552 |
| Description | The MHC region contains many genes that are key regulators of both innate and adaptive immunity including the polymorphic MHCI and MHCII genes. Consequently, the characterisation of the repertoire of MHC genes is critical to understanding the variation that determines the nature of immune responses. Currently our knowledge of the bovine MHCI repertoire is limited with only the Holstein-Friesian breed having been studied in any depth. Traditional methods of MHCI genotyping are of low resolution and laborious and this has been a major impediment to a more comprehensive analysis of the MHCI repertoire of other cattle breeds. Next-generation sequencing (NGS) technologies have been used to enable high throughput and much higher resolution MHCI typing in a number of species. In this study we have developed a MiSeq platform approach and requisite bioinformatics pipeline to facilitate typing of bovine MHCI repertoires. The method was validated initially on a cohort of Holstein-Friesian animals and then demonstrated to enable characterisation of MHCI repertoires in African cattle breeds, for which there was limited or no available data. During the course of these studies we identified >140 novel classical MHCI genes and defined 62 novel MHCI haplotypes, dramatically expanding the known bovine MHCI repertoire. Construction protocol: Total RNAs were extracted from white blood cell pellets prepared form blood obtained by venepuncture using Tri-reagent (Sigma-Aldrich, Dorset) and cDNA prepared using the Reverse Transcription Kit (Promega, WI); both according to the manufacturer's protocols. PCR amplification was performed with the Phusion HF PCR kit (NEB, Herts.) using bovine MHCI primers that included Illumina sequencing primer annealing sites, multiple sample identifier tags (D501-508 and D701-712) and Illumina ligation adaptors. PCR products from ~96 animals were pooled to form a library which was then sequenced on the Illumina Miseq platform. |
| Type Of Material | Database/Collection of data |
| Year Produced | 2016 |
| Provided To Others? | Yes |
| Impact | These data represent a reference dataset of novel MHC alleles in cattle and may underpin research into cattle immunogenetic diversity for many years in the future |
| URL | https://www.ebi.ac.uk/ena/data/view/PRJEB14552 |
| Title | PRJEB14979 |
| Description | Marek's Disease Virus (MDV) is a widespread alphaherpesvirus of poultry that causes Marek's disease (MD) characterised by fatal visceral CD4+ TCRaß+ T cell tumours at high incidence in susceptible hosts. As is the case with many virus-induced tumours, immortal cell lines harbouring viral genome have been generated from ex vivo cultures of MD tumours. As readily-available sources of large numbers of cells of a uniform type, MDV-transformed lymphoblastoid cell lines (LCL) have proved extremely valuable in studying virus-host interaction. While the viral genome is held in a latent state in most of the cells, a minor population of cells display spontaneous reactivation identifiable by the expression of lytic viral genes such as pp38. The process of spontaneous reactivation in these cell populations offers the opportunity for investigating the biological processes involved in the reactivation events. For this, we used two lymphoblastoid cell lines derived from lymphomas induced by pRB1B-UL47eGFP, a recombinant MDV engineered to express EGFP fused with the UL47. We used Fluorescence-activated cell sorting (FACS) to purify the rare EGFP-positive cell population with spontaneously activating viral genome from the majority EGFP-negative cells and analysed their gene expression profiles by RNA-sequencing using Illumina HiSeq2500. The reads generated were mapped using TopHat and gene expression levels were analysed by edgeR. Ingenuity pathway analysis software on more than 2000 differentially-expressed genes between the lytically infected (EGFP-positive) and latently infected (EGFP-negative) cell populations was used identify major biological pathways involved in the reactivation. These studies revealed that amongst others, transcripts directly involved in T activation such as CD3 complex, CD28, ICOS, phospholipase C, CD3 complex were down-regulated following reactivation of the virus in the LCL. |
| Type Of Material | Database/Collection of data |
| Year Produced | 2017 |
| Provided To Others? | Yes |
| Impact | These data detail the gene expression changes during spontaneous activation of MDV and will be useful to researchers investigating the mechanism of action of this important virus, as well as in vaccine design |
| URL | https://www.ebi.ac.uk/ena/data/view/PRJEB14979 |
| Description | Centre for Tropical Livestock Genetics and Health (CTLGH) |
| Organisation | International Livestock Research Institute (ILRI) |
| Country | Kenya |
| Sector | Charity/Non Profit |
| PI Contribution | Research contribution to the CTLGH result framework. |
| Collaborator Contribution | Research links and collaboration with its member partners in the UK and Africa. |
| Impact | No output to report yet. |
| Start Year | 2017 |
| Description | Centre for tropical livestock genetics and health (CTLGH) |
| Organisation | International Livestock Research Institute (ILRI) |
| Country | Kenya |
| Sector | Charity/Non Profit |
| PI Contribution | Mick Watson is co-leading programme 5 (informatics and bioresources) programme of CTLGH which aims to collect data on genotype and phenotype into a central database which can subsequently be mined for useful associations. So far we have ensured that scientists have access to the latest high performance computing environment for research, we have analysed and continue to analyse hundreds of farm animal genomes from LMIC countries, and we have built the data portal (http://data.ctlgh.org) |
| Collaborator Contribution | The partners are involved in all programmes, which include:Program 1: Harnessing genetic variability among indigenous and exotic breeds of cattle (as well as their crosses) to develop genetic and genomic tools that will be used to improve productivity under harsh tropical conditions and to mitigate the impact of cattle on climate change. Program 2: Harnessing genetic variability in tropical productivity and adaptation among various breeds of Chickens. Program 3: Development and application of precision breeding (through novel reproductive and germplasm technologies) to achieve step changes in livestock genetic improvement. Program 4: Understanding the genetic and molecular mechanisms underlying the tolerance of certain cattle and poultry breeds to tropical diseases and pests. Program 5: A shared global data and biological sample resource to support continued research and development on tropical livestock genetics and health. |
| Impact | The data portal so far: http://data.ctlgh.org The collaboration is multi-disciplinary, bringing together geneticists, parasitologists, virologists, epidemiologists and data scientists together. |
| Start Year | 2016 |
| Description | Centre for tropical livestock genetics and health (CTLGH) |
| Organisation | Scotland's Rural College |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Mick Watson is co-leading programme 5 (informatics and bioresources) programme of CTLGH which aims to collect data on genotype and phenotype into a central database which can subsequently be mined for useful associations. So far we have ensured that scientists have access to the latest high performance computing environment for research, we have analysed and continue to analyse hundreds of farm animal genomes from LMIC countries, and we have built the data portal (http://data.ctlgh.org) |
| Collaborator Contribution | The partners are involved in all programmes, which include:Program 1: Harnessing genetic variability among indigenous and exotic breeds of cattle (as well as their crosses) to develop genetic and genomic tools that will be used to improve productivity under harsh tropical conditions and to mitigate the impact of cattle on climate change. Program 2: Harnessing genetic variability in tropical productivity and adaptation among various breeds of Chickens. Program 3: Development and application of precision breeding (through novel reproductive and germplasm technologies) to achieve step changes in livestock genetic improvement. Program 4: Understanding the genetic and molecular mechanisms underlying the tolerance of certain cattle and poultry breeds to tropical diseases and pests. Program 5: A shared global data and biological sample resource to support continued research and development on tropical livestock genetics and health. |
| Impact | The data portal so far: http://data.ctlgh.org The collaboration is multi-disciplinary, bringing together geneticists, parasitologists, virologists, epidemiologists and data scientists together. |
| Start Year | 2016 |
| Description | Collaboration with AB Vista |
| Organisation | AB Vista |
| Country | United Kingdom |
| Sector | Private |
| PI Contribution | We are researching the activity of ovodefensins, naturally-occurring egg peptides with antimicrobial properties. With BBSRC LINK funding we are exploring the activity of diverse ovodefensins from multiple avian species, dissecting their structure and mode of action, and exploring their capacity to act as growth promoters and novel therapeutics. |
| Collaborator Contribution | AB Vista have made a substantial cash contribution to the LINK project (c. £460k) and also provide access to natural populations of broilers for feed trials using diets supplemented (or not) with Trichoderma-expressed ovodefensins. They also support analysis of the impact of such diets on intestinal microbiota and metabolites. |
| Impact | Commercially sensitive and to be disclosed following scrutiny for Intellectual Property. |
| Start Year | 2012 |
| Description | Collaboration with Boehringer Ingelheim |
| Organisation | Boehringer Ingelheim |
| Country | Germany |
| Sector | Private |
| PI Contribution | The collaboration is to advance diagnostics in relation to specific bacterial infections in pigs. We are carrying out basic science investigating antigens and immune responses |
| Collaborator Contribution | The company is providing reagents and expertise in this subject area |
| Impact | Part-funded PhD studentship |
| Start Year | 2017 |
| Description | India DBT collaboration |
| Organisation | Indian Council of Agricultural Research |
| Department | National Institute of High Security Animal Diseases |
| Country | India |
| Sector | Public |
| PI Contribution | Using the tissues collected in Bhopal, we have sequenced and analysed the avian transcriptomic response to avian influenza challenge in 6 bird species (chicken, turkey, crow, goose, pigeon, duck). |
| Collaborator Contribution | All influenza challenge experiments were carried out by the team in Bhopal, tissues collected and sent over to the UK for processing and analysis |
| Impact | Identification of candidate genes for resistance to avian flu. |
| Start Year | 2014 |
| Description | PRRS Host Genetics Consortium |
| Organisation | Iowa State University |
| Country | United States |
| Sector | Academic/University |
| PI Contribution | Genetic analysis of infectious disease data and mathematical modelling of PRRS infection dynamics; Contribution to experimental design of PRRS challenge and field experiments and sampling; |
| Collaborator Contribution | Access to data; access to statistical models; scientific advice for model assumptions |
| Impact | Joint manuscripts and publications: 1. Go N., Islam Z., Lunney J., Belloc C., Touzeau S.& Doeschl-Wilson A.B., 2017. Neutralizing antibodies prevent PRRS viremia rebound: evidence from a data-supported model of the immune response. PloS Comp. Biol. Under review 2. Lough G., Hess A., Dekkers JCM, Hess M., Kyriazakis I., Mulder H., Lunney J, Rowland BB, Doeschl-Wilson A.B. 2017. Harnessing longitudinal information to identify genetic variation in tolerance of pigs to Porcine Reproductive and Respiratory Syndrome virus. Genetics Selection Evolution. Under review 3. Lough G., Rashidi H., Kyriazakis I., Dekkers JCM, Hess AS, Hess MK, Deeb N, Kause A., Lunney J, Rowland BB, Mulder H and Doeschl-Wilson A.B. 2017. Use of multi-trait and random regression models to identify genetic variation in tolerance to Porcine Reproductive and Respiratory Syndrome virus. Genetics Selection Evolution, 49(1)37. 3. 4. Hess, A.S., Islam, Z., Hess, M.K., Rowland, R.R., Lunney, J.K., Doeschl-Wilson, A., Plastow, G.S. and Dekkers, J.C., 2016. Comparison of host genetic factors influencing pig response to infection with two North American isolates of porcine reproductive and respiratory syndrome virus. Genetics Selection Evolution, 48(1), p.1.18. 5. Islam Z.U., Bishop S.C., Savill N.J. , Rowland R.R.R., Lunney J.K., Trible B and Doeschl-Wilson A.B. 2013. Quantitative analysis of Porcine Reproductive and Respiratory Syndrome (PRRS) viremia profiles from experimental infection: a statistical modelling approach. PLoS One, DOI: 10.1371/journal.pone.0083567. ( |
| Start Year | 2012 |
| Description | Use of MHC elution to define functional parameters of bovine MHC haplotypes |
| Organisation | Technical University of Denmark |
| Country | Denmark |
| Sector | Academic/University |
| PI Contribution | We provide the biological context for the studies, prepare laboratory materials for analysis and subsequent in vitro validation of the data generated by our collaborators |
| Collaborator Contribution | The Jenner Institute perform the tandem nPLC-mass spectrometry and preliminary peptide identification. The Technical University of Denmark performs subsequent bioinformatics analysis. |
| Impact | Publication - PMID: 29115832. Novel information on the peptide-binding motifs of bovine MHCI molecules. Preliminary data for granny submission. Collaboration to support a post-graduate Brazilian student to undertake a 9 month FAPESP-funded scholarship at the collaborating laboratories. |
| Start Year | 2015 |
| Description | Use of MHC elution to define functional parameters of bovine MHC haplotypes |
| Organisation | University of Oxford |
| Department | Jenner Institute |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | We provide the biological context for the studies, prepare laboratory materials for analysis and subsequent in vitro validation of the data generated by our collaborators |
| Collaborator Contribution | The Jenner Institute perform the tandem nPLC-mass spectrometry and preliminary peptide identification. The Technical University of Denmark performs subsequent bioinformatics analysis. |
| Impact | Publication - PMID: 29115832. Novel information on the peptide-binding motifs of bovine MHCI molecules. Preliminary data for granny submission. Collaboration to support a post-graduate Brazilian student to undertake a 9 month FAPESP-funded scholarship at the collaborating laboratories. |
| Start Year | 2015 |
| Description | Annual Roslin Institute Open Doors Day |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Public/other audiences |
| Results and Impact | The annual Roslin Institute Open Doors day was held on 14 October 2017 and was attended by 491 people. It involved 81 members of staff and students and showcased a wide range of research arising from BBSRC strategic investment, including (but not limited to), food safety, influenza, genetic improvement, DNA, imaging and the ethical treatment of animals in research. |
| Year(s) Of Engagement Activity | 2017 |
| Description | Annual Roslin Institute Open Doors Day |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Public/other audiences |
| Results and Impact | The annual Roslin Institute Open Doors day was held on 14 October 2017 and was attended by 491 people. It involved 81 members of staff and students and showcased a wide range of research arising from BBSRC strategic investment, including (but not limited to), food safety, influenza, genetic improvement, DNA, imaging and the ethical treatment of animals in research. |
| Year(s) Of Engagement Activity | 2017 |
| Description | BBC Countryfile film related to the work of the Centre for Tropical Livestock Genetics & Health at Roslin |
| Form Of Engagement Activity | A broadcast e.g. TV/radio/film/podcast (other than news/press) |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Public/other audiences |
| Results and Impact | Bill Gates was interviewed by BBC Countryfile on 26 January 2018 in relation to work funded by the Gates Foundation involving Roslin Institute scientists towards the genetic improvement of farmed animals in low- and middle-income countries. The programme aired on 11 February 2018 and reached an estimated audience in excess of 5 million. The interview took place on the same day as the launch of the Global Academy of Agriculture & Food Security, which was attended by the Secretary of State for the Department for International Development the Rt Hon Penny Mordaunt (see separate activity) and generated significant press interest, featuring on BBC Scotland news. Particular emphasis was placed on the work of Roslin scientists to understand the genetic make-up of African diary cattle and chickens and to understand how this relates to performance traits in order to guide breeding decisions. |
| Year(s) Of Engagement Activity | 2018 |
| Description | BBSRC-DBT Livestock Health and Disease workshop (2012), Delhi, India in Feb 2012 |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | BBSRC-DBT Livestock Health and Disease workshop (2012), Delhi, India in Feb 2012 no actual impacts realised to date |
| Year(s) Of Engagement Activity | 2012 |
| Description | British society for Immunology |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Postgraduate students |
| Results and Impact | Poster presentation at the British Society for Immunology, Dec 2017. Poster entitled: Precision cut lung slices: A platform for examining host-pathogen interactions in an avian model. Authors on the posters: Bryson K, Esposito M., Lamont S., Stevens M., McLaughlan G. and Vervelde L. |
| Year(s) Of Engagement Activity | 2017 |
| Description | Edinburgh India Institute, Inaugural conference on 15-16 May 2014 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Policymakers/politicians |
| Results and Impact | Gave a short talk about avian influenza BBSRC-DBT project and how it came about, joint talk with our India collaborators. Edinburgh India Institute, Inaugural conference on 15-16 May 2014. John McIntyre Conference Centre, Edinburgh. Conference theme: Innovative Engagement for Sustainable Development: the Edinburgh-India Story. |
| Year(s) Of Engagement Activity | 2014 |
| Description | Flu talk - journalists |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Media (as a channel to the public) |
| Results and Impact | Presentation of our work on avian flu to a selection of Indian and Japanese journalists. Government representatives were also present. Interest in future Scottish/Indian proposals was shown. |
| Year(s) Of Engagement Activity | 2015 |
| Description | ISAG talk |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Postgraduate students |
| Results and Impact | Talk title: Mapping QTLs affecting Marek's disease by selective DNA pooling in eight lines across 15 generations |
| Year(s) Of Engagement Activity | 2017 |
| Description | Introduction to the Research of the Roslin Institute - Royal Society of Biology Scottish Branch Annual Symposium |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Professional Practitioners |
| Results and Impact | Professor Stevens provided an overview of the research of The Roslin Institute to members of the public and the Royal Society of Biology, who convened for the annual symposium of the RSB Scottish Branch on 14 October 2017 at Roslin. The event coincided with the annual Roslin 'Open Doors' day and featured BBSRC-funded research. |
| Year(s) Of Engagement Activity | 2017 |
| Description | Invited speaker - Avian Genetics and Immunity Symposium |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Research on Avian Genetics and Immunity (RAGI) has been established to strengthen global partnerships and links between academic researchers, students and the poultry professionals working on the genetics of avian immunity. The purpose of the symposium was to provide a forum for discussion and exchange of ideas around the single theme of exploiting available opportunities in ultimately dealing emerging and existing infectious avian diseases in poultry production systems and to safeguard food supplies and human health. The purpose of KW talk was to overview the current situation in industry for breeding for disease resistance and introduce the NARF. The overview of NARF included a review of facilities, lines available and current work being carried out using NARF avian lines. The presentation stimulated significant discussion. |
| Year(s) Of Engagement Activity | 2017 |
| URL | http://geneticsandimmunity.com/ |
| Description | Invited talk at symposium |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Industry/Business |
| Results and Impact | North American PRRS symposium. Chicago, USA, Dec 2017. Novel insights about host response to PRRS from data driven mathematical models. Invited talk. |
| Year(s) Of Engagement Activity | 2017 |
| Description | Munster flu meeting |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | 5th international influenza meeting. IFITM talk. Munster, Germany. Sep 2016 |
| Year(s) Of Engagement Activity | 2016 |
| Description | Open lecture on African trypanosomes in cattle to Royal Society of Biology Scottish Branch |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Professional Practitioners |
| Results and Impact | Dr Liam provided an overview of his research on African trypanosomes in cattle to members of the public and the Royal Society of Biology, who convened for the annual symposium of the RSB Scottish Branch on 14 October 2017 at Roslin. The event coincided with the annual Roslin 'Open Doors' day and featured BBSRC-funded research. |
| Year(s) Of Engagement Activity | 2017 |
| Description | PAG 2018 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Invited speaker at Plant and Animal Genome conference 2018 (San Diego, USA) 'Avian flu: the winners and the losers' - Jan 2018 |
| Year(s) Of Engagement Activity | 2018 |
| Description | Poultry Breeders Roundtable |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | • Invited speaker at National poultry breeders roundtable meeting (St Louis, USA) - JS May 2017 |
| Year(s) Of Engagement Activity | 2017 |
| Description | Public lecture - Inaugural Lecture of Professor Tanja Opriessnig |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Schools |
| Results and Impact | Professor Opriessnig delivered her Inaugual Lecture on 'From 'circus virus' to mostly commonly used swine vaccine on 24 April 2017, relating to her BBSRC-funded research on porcine viral diseases and strategies to detect and control them. The event was attended by children from local schools, members of the public and students of the University of Edinburgh. |
| Year(s) Of Engagement Activity | 2017 |
| Description | Public lecture entitled Confronting the Microbial Menace in Our Food'. Professor Mark Stevens |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Schools |
| Results and Impact | The inaugural lecture of Professor Mark Stevens was held on 30 October 2017 on his BBSRC-funded research to identify bacterial and host factors influencing the ability of Salmonella, Campylobacter and E. coli to colonise farm animals and cause disease. It was attended by children from local schools, members of the public, students at The Roslin Institute and wider University of Edinburgh and posted online. |
| Year(s) Of Engagement Activity | 2017,2018 |
| URL | https://media.ed.ac.uk/media/Inaugural+lectureA+Confronting+the+microbial+menace+in+our+food/1_x5k5e... |
| Description | Roslin flu meeting |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | 'Avian flu: the winners and the losers' at Annual influenza meeting, Roslin Nov 2017 |
| Year(s) Of Engagement Activity | 2017 |
| Description | Science Insights work experience for S5 school pupils |
| Form Of Engagement Activity | Participation in an open day or visit at my research institution |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | We ran our Science Insights work experience programme for local school pupils on 24-28 July 2017. 38 S5 pupils attended, 20 of whom worked in the Roslin Institute for 2 days alongside BBSRC-supported researchers from this ISP, including our core-funded staff scientists. All 38 students also had a tour of the Langhill farm and attended a presentation and debate about the use of animals in research. Students also had the opportunity to discuss career paths and what it is like to work as a research scientist. |
| Year(s) Of Engagement Activity | 2017 |
| URL | https://www.ed.ac.uk/roslin/working/opportunities/young-people/science-insights |
| Description | Stand at the Royal Highland Show, including the BBSRC-funded Flu Fighters exhibit. |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Public/other audiences |
| Results and Impact | Roslin Institute stand at the Royal Highland Show 22-25 June 2017. Approximately 25 Roslin Institute staff and students were involved. Exhibits of relevance to this ISP include the BBSRC-funded Flu Fighters exhibit, highlighting the problem of influenza and our research to combat it. |
| Year(s) Of Engagement Activity | 2017 |
| Description | Summer course |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Postgraduate students |
| Results and Impact | Armidale Animal Breeding Summer Course 2018: Mathematical modeling of infection dynamics in genetically diverse livestock populations Teachers: Andrea Doeschl-Wilson and Osvaldo Anacleto Dates: Monday 5 February 2018 (9am) - Thursday 8 February 2018 (4pm) Venue: University of New England, Armidale, NSW Australia; ~40 post-graduate students, academics and industrial researcher attended the workshop in modelling. Based on the positive course evaluation, the course sparked new research in the important field of mathematical modelling of infection dynamics and genetics |
| Year(s) Of Engagement Activity | 2018 |
| URL | https://jvanderw.une.edu.au/aabc2018.htm |
| Description | TEDx Talk by Professor Helen Sang on 'Why do we need GM chickens?' |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Public/other audiences |
| Results and Impact | Professor Helen Sang delivered a Tedx Talk in Glasgow on 2 June 2017 on 'Why do we need GM chickens?'. The event was attended by approximately 1700 people and has been shared online with over 900 views. It highlighted the challenge of achieving global food security and the potential of BBSRC-funded research to improve poultry, including via genetic alteration. Examples for ongoing research using GM poultry in this programme were shared. |
| Year(s) Of Engagement Activity | 2017 |
| URL | https://www.youtube.com/watch?v=DUbqrh5otWs |
| Description | The Human Body activities at a BBC-sponsored Edinburgh Festival event |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | Researchers from The Roslin Institute joined colleagues from across the University's College of Medicine and Veterinary Medicine in the grounds of George Heriot's School in central Edinburgh to deliver family-friendly activities about 'The Human Body' on 15 August 2017. The event was part of a series of science-themed activities hosted by the BBC at the Edinburgh Festivals to support BBC Learning's 'Terrific Scientific' campaign, which aims to inspire children and young people to engage with science. Our staff and Ph.D students ran interactive activities about their research on lungs and skin, including inflating and examining real pig lungs, comparing healthy and diseased organs, and a chance for visitors to use their craft skills to make a model of their skin. Other activities at the event included anatomy face and body painting and a chance to see dissections of animal brains and hearts close-up. An estimated 1,400 family visitors were engaged, with great questions about our research and what it's like to work as a scientist. |
| Year(s) Of Engagement Activity | 2017 |
| URL | https://www.ed.ac.uk/roslin/community-engagement/public-events/events-archive/the-human-body-bbc-at-... |
| Description | Visit by Bill Gates and the Secretary of State for the Department of International Development |
| Form Of Engagement Activity | A press release, press conference or response to a media enquiry/interview |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Public/other audiences |
| Results and Impact | Bill Gates and the Secretary of State for the Department of International Development (DfID; Rt Hon Penny Mordaunt) visited The Roslin Institute on 26 January 2018. This involved separate sessions with the Gates Foundation and DfID to learn about the research undertaken by the Centre for Tropical Livestock Genetics & Health (CTLGH), in which the Roslin Institute is a major partner and researchers in the Institute Strategic Programmes are heavily involved. DfID announced £4m of funding for the CTLGH, adding to £10m already received from the Gates Foundation. In the presence of the Principal of the University of Edinburgh, Bill Gates and the Rt Hon Penny Mordaunt formally opened the Global Academy for Agriculture & Food Security which will provide undergraduate and postgraduate training in sustainable agriculture. The event took place in the new Campus Innovation Building (which received BBSRC strategic funding). The University of Edinburgh press office tracked the following activity related to the visit, in which many participants in the ISPs were involved: • 109 individual articles and broadcast pieces that mentioned the University with regards to the visit, with an estimated potential reach of more than 7 million people. • Of these, more than half of the coverage mentioned one of more of the Global Academy of Agriculture & Food Security, the Centre for Tropical Livestock Genetics & Health, SEBI, the Royal (Dick) School of Veterinary Studies, Roslin or the Easter Bush Campus in addition to name checking the University. • In addition to the UK, we also attracted press coverage in the USA, France, New Zealand, China, Zimbabwe, South Africa, Botswana, Taiwan and Egypt. • Across social media, we reached more than 1.4 million people with 14k+ engagements (likes, shares, retweets etc). Of these, 99.8% were positive. • The Facebook Live streaming alone reached more than 100k people. More than 18k people have watched the video. • Our news videos have garnered more than 35k views across platforms. • We tracked a spike in traffic to the Global Academy website on the day of the visit - 1405 visits (11 times the average for the previous month). |
| Year(s) Of Engagement Activity | 2018 |
| Description | WGALP talk |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Postgraduate students |
| Results and Impact | Talk entitled 'QTLs mapped by selective DNA pooling explain substantial proportion of phenotypic and genetic variation in MD mortality in chicken layer lines' presented at WGALP in Auckland, Feb 2018 |
| Year(s) Of Engagement Activity | 2018 |