Novel strategies to control virus infection and disease

Lead Research Organisation: University of Edinburgh
Department Name: The Roslin Institute

Abstract

Endemic and exotic virus diseases are threats to food security and, in some cases, potential zoonoses. We aim to develop novel strategies to control virus infection, focussing on improving animal welfare and reducing the disease burden in food production animals. The objectives include i) development of vaccines based around pox virus and herpesvirus delivery systems; ii) exploitation of transgenesis as a means of delivering resistance genes including antiviral peptides. Preliminary studies suggest that it is possible to prevent or reduce virus transmission, infection or disease by genetic modification of mammalian/avian hosts; iii) development of the concept of a bioshield involving constitutive expression of antiviral peptides expressed by probiotic bacteria colonisation of the GI tract.

Publications

10 25 50

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Freeman TC (2012) A gene expression atlas of the domestic pig. in BMC biology

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Muratore G (2012) Small molecule inhibitors of influenza A and B viruses that act by disrupting subunit interactions of the viral polymerase. in Proceedings of the National Academy of Sciences of the United States of America

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Schroder K (2012) Conservation and divergence in Toll-like receptor 4-regulated gene expression in primary human versus mouse macrophages. in Proceedings of the National Academy of Sciences of the United States of America

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Nicol MQ (2012) A novel family of peptides with potent activity against influenza A viruses. in The Journal of general virology

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MacCormick IJ (2012) Retinal vessel tortuosity in response to hypobaric hypoxia. in High altitude medicine & biology

 
Description An objective was development of vaccines based around pox virus and herpesvirus delivery systems. In collaboration with Dr Michael Jarvis, University of Plymouth, we developed murine cytomegalovirus recombinants expressing IAV CTL epitopes and tested their ability to protect against lethal IAV infection in mice. Unfortunately, we were not able to demonstrate protection. (PIs, Dutia, Digard).

We also explored the development of a novel antiviral peptide, FluPep which we showed had antiviral activity in vitro and in vivo could protect against a lethal influenza A virus (IAV) infection. Although we were able to obtain a small amount of funding from Apeptico and also approached larger companies (Roche), we were not able to obtain sufficient funds to develop this commercially. (PIs Dutia, Nash, Digard).

Our discovery of the PA-X pathogenicity-determining gene in IAV has led to the further findings that removing PA-X expression from the PR8 strain of the virus decreases pathogenicity in embryonated hens eggs. This is important because (a) 100s of millions of hens eggs are used annually to grow flu vaccine shots based on the PR8 virus an (b) we have found that this reduction in viral pathogenicity can increase the yield of vaccine from otherwise poor-yielding strains. (PI Digard).
We have used CRISPR/Cas9 editing in pig zygotes, resulting in the generation of pigs with a deletion of Exon 7 of the CD163 gene, encoding SRCR5. Deletion of SRCR5 showed no adverse effects in pigs maintained under standard husbandry conditions with normal growth rates and complete blood counts observed. Macrophages isolated from the animals show the characteristic differentiation and cell surface marker expression of macrophages of the respective origin. Expression and correct folding of the SRCR5 deletion CD163 on the surface of macrophages and biological activity of the protein as hemoglobin-haptoglobin scavenger was confirmed. In vitro challenge of macrophages with PRRSV-1, subtypes 1, 2, and 3 with PRRSV-2 showed complete resistance to viral infections assessed by replication. In vivo infection analysis with a PRRSV-1 subtype 2 strain showed complete resistance in the pig as well and further confirmed biological function of the CD163 SRCR5 deletion variant by expression of the protein in its soluble form in the serum of these animals.
Exploitation Route We are actively trying to commercialise the PA-X based method for improving vaccine yield. Patent protection has been sought (Improved flu vaccine yield. P. Digard, The University Court of the University of Edinburgh. UK patent application no 1602535.5 (22/2/16).) and our business development team are in contact with potential industrial partners. Commercialisation of the PRRSV-resistant pigs is being actively pursued with commercial partners.
Sectors Agriculture, Food and Drink,Healthcare

 
Description BBSRC strategic investment at Roslin via this and other Institute Strategic Programmes has sustained expertise and facilities that have attracted substantial inward investment at Easter Bush. This has recently included £15M Gates Foundation funding to establish the Centre for Tropical Livestock Genetics & Health to implement genetic gain and treatments in support of farmers in low- and middle-income farmers, including to control endemic and zoonotic diseases. It has also driven dozens of productive collaborations with industry via contracts and leveraging schemes, leading to £10M private equity investment to form Roslin Technologies to commercialise our research and construction of a new £32M Innovation Centre adjacent to the Institute to engage and grow businesses. Moreover, the expertise supported by this funding has attracted major investment in Agri-Tech Centres at Easter Bush funded by InnovateUK (Centre for Innovation Excellence in Livestock & Agri-EPI). These Centres will increase national capacity for infectious challenge in farmed animals, gene editing and collection of novel phenotypes and be linked to stakeholders throughout the food supply chain. Specific examples of impact are: Our Porcine Epidemic Diarrhea Virus S1 diganostic ELISA was the first of its kind and Hipra, Spain (www.hipra.com) has taken the method and our construct on board and now have offer a PEDV ELISA kit for sale.
First Year Of Impact 2016
Sector Agriculture, Food and Drink,Education,Healthcare
 
Description Barriers to Rabies vaccination in Malawi
Geographic Reach Africa 
Policy Influence Type Influenced training of practitioners or researchers
Impact Using a basic regression model distance to vaccination point was identified as one of the main barriers to people to bring their dogs and cats for vaccination. Having identified the issue we were able to advise Mission Rabies and the Malawi government on the better location of vaccination points in 2018.
 
Description Expert group member on coronavirus for for DISCONTOOLS
Geographic Reach Europe 
Policy Influence Type Implementation circular/rapid advice/letter to e.g. Ministry of Health
Impact The policy is currently in work.
 
Description Royal Society Contact Group on Gene Editing Technologies
Geographic Reach National 
Policy Influence Type Participation in a advisory committee
 
Description BBSRC Animal Health Research Club
Amount £94,126 (GBP)
Funding ID BB/M502972/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 10/2014 
End 09/2020
 
Description BBSRC Animal Health Research Club
Amount £94,126 (GBP)
Funding ID BB/M502984/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 10/2014 
End 09/2018
 
Description CALL: INTEGRATING AND OPENING RESEARCH INFRASTRUCTURES OF EUROPEAN INTEREST
Amount € 10,000,000 (EUR)
Funding ID H2020-INFRAIA-2016-1 project number 731014 
Organisation European Commission 
Sector Public
Country European Union (EU)
Start 04/2017 
End 03/2022
 
Description CASE studentship
Amount £90,000 (GBP)
Funding ID BB/L004666/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 09/2013 
End 08/2017
 
Description Dissecting the molecular pathways of MDV oncoprotein Meq for understanding pathogenesis and aid vaccine development
Amount £384,966 (GBP)
Funding ID BB/R007632/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 04/2018 
End 03/2021
 
Description EU H2020
Amount € 5,500,000 (EUR)
Organisation European Commission 
Department Horizon 2020
Sector Public
Country European Union (EU)
Start  
 
Description Host targeted microRNA therapeutics for the treatment of viral infections
Amount £779,224 (GBP)
Organisation Scottish Enterprise 
Sector Public
Country United Kingdom
Start 08/2013 
End 08/2015
 
Description Porcine antiviral studies
Amount £91,000 (GBP)
Funding ID R1840 
Organisation Eco Animal Health Ltd 
Sector Private
Country United Kingdom
Start 06/2017 
End 12/2018
 
Description Research Grant - Christine Tait-Burkard - Nanobodies as antiviral PRRSV agents
Amount £59,802 (GBP)
Funding ID Roslin Contracts Ref 2191 
Organisation Eco Animal Health Ltd 
Sector Private
Country United Kingdom
Start 03/2018 
End 03/2019
 
Description Research Grant - Paul Digard - Tylvalosin as a porcine antiviral compound
Amount £90,833 (GBP)
Funding ID Roslin 1840 
Organisation Eco Animal Health Ltd 
Sector Private
Country United Kingdom
Start 03/2017 
End 09/2018
 
Description Sir Henry Dale Fellowship - Eleanor Gaunt - The role of CpG dinucleotides in regulating virus replication kinetics
Amount £1,025,674 (GBP)
Funding ID 211222/Z/18/Z 
Organisation Wellcome Trust 
Sector Charity/Non Profit
Country United Kingdom
Start 10/2018 
End 09/2023
 
Description Vacation Scholarship - Paul Digard - Mechanisms of antiviral drug resistance in influenza A virus
Amount £2,000 (GBP)
Funding ID 208707/Z/17/Z 
Organisation Wellcome Trust 
Sector Charity/Non Profit
Country United Kingdom
Start 07/2017 
End 08/2017
 
Description contract award
Amount $4,766,000 (USD)
Funding ID BARDA_BAA-11-100-SOL-00001 
Organisation Biomedical Advanced Research and Development Authority 
Sector Public
Country United States
Start 01/2013 
End 03/2017
 
Title CDCD pig model 
Description We improved a caesarean-derived, colostrum deprived pig model to ensure that animal welfare and survivability of pigs increases. 
Type Of Material Improvements to research infrastructure 
Year Produced 2014 
Provided To Others? No  
Impact Survivability of pigs increased from 50% to 90-100% 
 
Title ELISpot for PRRSV 
Description An ELISpot for PRRSV was developed to investigate cellular immune responses in vaccine development studies. 
Type Of Material Technology assay or reagent 
Year Produced 2018 
Provided To Others? No  
Impact The tool is essential to do vaccine efficacy studies. 
 
Title In silico generation of genome wide guide sequences for pig and chicken 
Description One of the aims of the project is to design CRISPR guide sequences against each of the annotated genes for pig and chicken using the most advanced algorithms available. this has now been achieved and selected guide sequences are being test in pig and chicken cell lines to determine efficacy. Once the full libraries have been validated the guide sequences will be made publicly available through dedicated pig and chicken genome websites. 
Type Of Material Technology assay or reagent 
Provided To Others? No  
Impact Once publicly available, any researcher will be able to search for guide sequences to their gene of interest, allowing rapid generation of guide sequences to generate knockout libraries. 
 
Title PDCoV-ELISA 
Description A test to develop antibodies to PDCoV IgA and IgG antibodies for early detection of infection was developed. 
Type Of Material Technology assay or reagent 
Year Produced 2014 
Provided To Others? Yes  
Impact The test is used for early detection of infection of a pig herd with PDCoV. 
 
Title PEDV assay 
Description An ELISA for detection of IgG and IgA PEDV antibodies was developed. 
Type Of Material Technology assay or reagent 
Year Produced 2014 
Provided To Others? Yes  
Impact Test is used to determine if pig herds are free of PEDV 
 
Title PRJEB14979 
Description Marek's Disease Virus (MDV) is a widespread alphaherpesvirus of poultry that causes Marek's disease (MD) characterised by fatal visceral CD4+ TCRaß+ T cell tumours at high incidence in susceptible hosts. As is the case with many virus-induced tumours, immortal cell lines harbouring viral genome have been generated from ex vivo cultures of MD tumours. As readily-available sources of large numbers of cells of a uniform type, MDV-transformed lymphoblastoid cell lines (LCL) have proved extremely valuable in studying virus-host interaction. While the viral genome is held in a latent state in most of the cells, a minor population of cells display spontaneous reactivation identifiable by the expression of lytic viral genes such as pp38. The process of spontaneous reactivation in these cell populations offers the opportunity for investigating the biological processes involved in the reactivation events. For this, we used two lymphoblastoid cell lines derived from lymphomas induced by pRB1B-UL47eGFP, a recombinant MDV engineered to express EGFP fused with the UL47. We used Fluorescence-activated cell sorting (FACS) to purify the rare EGFP-positive cell population with spontaneously activating viral genome from the majority EGFP-negative cells and analysed their gene expression profiles by RNA-sequencing using Illumina HiSeq2500. The reads generated were mapped using TopHat and gene expression levels were analysed by edgeR. Ingenuity pathway analysis software on more than 2000 differentially-expressed genes between the lytically infected (EGFP-positive) and latently infected (EGFP-negative) cell populations was used identify major biological pathways involved in the reactivation. These studies revealed that amongst others, transcripts directly involved in T activation such as CD3 complex, CD28, ICOS, phospholipase C, CD3 complex were down-regulated following reactivation of the virus in the LCL. 
Type Of Material Database/Collection of data 
Year Produced 2017 
Provided To Others? Yes  
Impact These data detail the gene expression changes during spontaneous activation of MDV and will be useful to researchers investigating the mechanism of action of this important virus, as well as in vaccine design 
URL https://www.ebi.ac.uk/ena/data/view/PRJEB14979
 
Description Biochemical interaction of CD163 with PRRSV glycoproteins GP2, GP3, and GP4 
Organisation Free University of Berlin
Country Germany 
Sector Academic/University 
PI Contribution We are providing purified, tagged CD163 protein variations for the assessment of biochemical and biophysical interactions of the protein with the PRRSV glycoproteins GP2, GP3, and GP4 and glycoprotein complex GP2/3/4.
Collaborator Contribution Our collaboration partners area analysing the biochemical and biophysical interactions of CD163 variants with the PRRSV glycoproteins GP2, GP3, and GP4 and glycoprotein complex GP2/3/4 using their established baculoviral expression system.
Impact So far we are in the early stages of this collaboration and many protocols are still being optimised.
Start Year 2018
 
Description Centre for tropical livestock genetics and health (CTLGH) 
Organisation International Livestock Research Institute (ILRI)
Country Kenya 
Sector Charity/Non Profit 
PI Contribution Mick Watson is co-leading programme 5 (informatics and bioresources) programme of CTLGH which aims to collect data on genotype and phenotype into a central database which can subsequently be mined for useful associations. So far we have ensured that scientists have access to the latest high performance computing environment for research, we have analysed and continue to analyse hundreds of farm animal genomes from LMIC countries, and we have built the data portal (http://data.ctlgh.org)
Collaborator Contribution The partners are involved in all programmes, which include:Program 1: Harnessing genetic variability among indigenous and exotic breeds of cattle (as well as their crosses) to develop genetic and genomic tools that will be used to improve productivity under harsh tropical conditions and to mitigate the impact of cattle on climate change. Program 2: Harnessing genetic variability in tropical productivity and adaptation among various breeds of Chickens. Program 3: Development and application of precision breeding (through novel reproductive and germplasm technologies) to achieve step changes in livestock genetic improvement. Program 4: Understanding the genetic and molecular mechanisms underlying the tolerance of certain cattle and poultry breeds to tropical diseases and pests. Program 5: A shared global data and biological sample resource to support continued research and development on tropical livestock genetics and health.
Impact The data portal so far: http://data.ctlgh.org The collaboration is multi-disciplinary, bringing together geneticists, parasitologists, virologists, epidemiologists and data scientists together.
Start Year 2016
 
Description Centre for tropical livestock genetics and health (CTLGH) 
Organisation Scotland's Rural College
Country United Kingdom 
Sector Academic/University 
PI Contribution Mick Watson is co-leading programme 5 (informatics and bioresources) programme of CTLGH which aims to collect data on genotype and phenotype into a central database which can subsequently be mined for useful associations. So far we have ensured that scientists have access to the latest high performance computing environment for research, we have analysed and continue to analyse hundreds of farm animal genomes from LMIC countries, and we have built the data portal (http://data.ctlgh.org)
Collaborator Contribution The partners are involved in all programmes, which include:Program 1: Harnessing genetic variability among indigenous and exotic breeds of cattle (as well as their crosses) to develop genetic and genomic tools that will be used to improve productivity under harsh tropical conditions and to mitigate the impact of cattle on climate change. Program 2: Harnessing genetic variability in tropical productivity and adaptation among various breeds of Chickens. Program 3: Development and application of precision breeding (through novel reproductive and germplasm technologies) to achieve step changes in livestock genetic improvement. Program 4: Understanding the genetic and molecular mechanisms underlying the tolerance of certain cattle and poultry breeds to tropical diseases and pests. Program 5: A shared global data and biological sample resource to support continued research and development on tropical livestock genetics and health.
Impact The data portal so far: http://data.ctlgh.org The collaboration is multi-disciplinary, bringing together geneticists, parasitologists, virologists, epidemiologists and data scientists together.
Start Year 2016
 
Description Collaboration with Karyopharm Therapeutics Ltd 
Organisation Karyopharm Therapeutics
Country United States 
Sector Private 
PI Contribution Karyopharm Therapeutics Ltd approached us over testing small molecule inhibitors of nuclear export selective for the cellular protein Crm1 (SINEs) as potential antiviral agents against influenza A virus. This project meshed nicely with our own research interests so we set up a research collaboration to do the work
Collaborator Contribution Karyopharm provided us with a small amount of consumable cash as well as the drugs themselves.
Impact The work has been presented at several national and international meetings and is currently being prepared for publication. It also provided the basis for Ms Dewar's PhD thesis.
Start Year 2013
 
Description ELANCO collaboration 
Organisation Eli Lilly & Company Ltd
Department Elanco
Country United States 
Sector Private 
PI Contribution Confidential
Collaborator Contribution Confidential
Impact Confidential
Start Year 2014
 
Description ISU collaboration 
Organisation Iowa State University
Country United States 
Sector Academic/University 
PI Contribution Providing knowhow and imput
Collaborator Contribution Providing laboratory and animal space
Impact Several publications
Start Year 2013
 
Description In vivo challenge of genome edited deltaSRCR5 pigs with PRRSV-1 
Organisation Genus plc
Country United Kingdom 
Sector Private 
PI Contribution Following the successful generation of pigs lacking domain 5 (SRCR5) of the CD163 and the promising in vitro results, showing primary cells of these pigs to be resistant against PRRSV infection we obtained further funding from Genus plc. to conduct an in vivo study. 4 delta SRCR5 pigs and 4 wild type pigs were challenged with PRRSV-1. We conducted the study and analysed the outcomes.
Collaborator Contribution Genus plc contributed funding to conduct the in vivo study to assess infectability of delta SRCR5 pigs with PRRSV-1
Impact We published the results of this study in a scientific publication, disseminated the results in a press release package, as well as presented the work at various scientific conferences and public engagement events.
Start Year 2016
 
Description Maintenance of delta SRCR5 pigs 
Organisation Genus plc
Country United Kingdom 
Sector Private 
PI Contribution Breeding and maintenance of the genome edited delta SRCR5 pig line from the end of "Engineering resistance to Porcine Reproductive and Respiratory Syndrome Virus (PRRSV)" (BB/L004143/1) and the beginning of "Understanding the CD163 - PRRS virus interaction to improve genetic engineering for resistance" (BB/R004463/1).
Collaborator Contribution Genus plc provided the funds for breeding and maintenance of the delta SRCR5 pigs.
Impact The outcomes of this project are directly interlinked with the outcomes of the grants mentioned above.
Start Year 2017
 
Description Roslin-Genus Cooperative Partnership Addendum 16 
Organisation Genus plc
Country United Kingdom 
Sector Private 
PI Contribution The Roslin Institute team conceived and executed the research project to test the hypothesis that gene-editing the pig CD163 gene would confer resistance to infection with PRRSV.
Collaborator Contribution The Genus team contributed to the project design and management.
Impact Paper published: Burkard et al. 2017. PLOS Pathogens 1392): e1006206
Start Year 2010
 
Description UM collaboration 
Organisation University of Maryland, College Park
Country United States 
Sector Academic/University 
PI Contribution Performing vaccine efficacy studies
Collaborator Contribution Preparing virus and vaccine virus stocks
Impact Several publications
Start Year 2013
 
Description USDA-SEPRL 
Organisation U.S. Department of Agriculture USDA
Department Agricultural Research Service
Country United States 
Sector Public 
PI Contribution The grant is a joint one with the USDA - the BBSRC contributes the UK half of the funding
Collaborator Contribution As above, the USDA also contributes direct funding to the partner laboratory (PIs Dr Darrell Kapcyzinski and Mary Pantin-Jackwood). Their laboratory also benefits from core funding that provides further in kind benefits to the project.
Impact Only tangible outcome so far are meeting abstracts.
Start Year 2015
 
Description VT collaboration 
Organisation Virginia Tech
Country United States 
Sector Academic/University 
PI Contribution Developing of a pig model and testing viruses.
Collaborator Contribution Developing vaccines
Impact Several publications
Start Year 2014
 
Description Zoetis collaboration 
Organisation Zoetis
Country United States 
Sector Private 
PI Contribution Development of the research idea, animal model and pathogen isolation
Collaborator Contribution Vaccine preparation
Impact None at this point.
Start Year 2013
 
Title Domain 5 of CD163 for use in antiviral compositions against PRRS, and transgenic animals 
Description The present invention relates to methods and compositions useful for the prevention and/or treatment of PRRS in animals, typically domestic pigs. The invention relates to proteins which comprise fragments of CD163, nucleic acid constructs encoding such proteins, and methods of modifying expression or activity of CD 163 in vivo. 
IP Reference WO2015011483 
Protection Patent application published
Year Protection Granted
Licensed No
Impact Steps along the way to converting this intellectual property into socio-economic impact including establishing regulatory approval, if necessary, for products from gene edited pigs to enter the food chain generally or in this specific example and agreeing licensing terms with appropriate players in the agri-food chain.
 
Title Enhanced expression 
Description The present invention relates to methods for enhancing the expression of proteins by modifying the nucleotide composition of the encoding nucleic acid. In particular it relates to methods for enhancing the expression of RNA expression vectors, by reducing the frequency of CpG and/or UpA dinucleotides. The invention also relates to nucleic acids modified in such a way, and to systems in which such nucleic acids are used. 
IP Reference GB1305361.6 
Protection Patent application published
Year Protection Granted 2013
Licensed Yes
Impact None yet
 
Title Peptides 
Description Identification of a panel of peptides which inhibit replication of influenza A virus in vitro and in vivo 
IP Reference WO2012013979 
Protection Patent application published
Year Protection Granted
Licensed No
Impact None yet
 
Title SWINE COMPRISING MODIFIED CD163 AND ASSOCIATED METHODS 
Description The present invention relates to genetically edited swine which produce CD163 protein in which the scavenger receptor cysteine-rich 5 (SRCR5) domain (also known as CD163 domain 5) has been deleted. Such swine have been found to be healthy and do not exhibit negative properties, and are resistant to PRRSV infection. CD163 expressed in the edited swine also demonstrates retention of the ability to function as a haemoglobin-haptoglobin scavenger. Methods of producing such swine are also provided. 
IP Reference WO2018073237 
Protection Patent application published
Year Protection Granted 2018
Licensed No
Impact The patent on hand describes methods by which to generate animals resistant to PRRSV by genome editing SRCR domain 5 of the porcine protein CD163.
 
Description Annual Roslin Institute Open Doors Day 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact The annual Roslin Institute Open Doors day was held on 14 October 2017 and was attended by 491 people. It involved 81 members of staff and students and showcased a wide range of research arising from BBSRC strategic investment, including (but not limited to), food safety, influenza, genetic improvement, DNA, imaging and the ethical treatment of animals in research.
Year(s) Of Engagement Activity 2017
 
Description Avian Pathogenesis Meeting, Shanghai Veterinary Research Institute. "Generation of genome wide CRISPR Cas9 libraries for pig and chicken" 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact The Avian Pathogenesis Meeting was organised to provide an opportunity for interaction and collaboration with veterinary scientists from China. I presented our work on genome-wide CRISPR/cas9 libraries for pig and chicken and ISG libraries for pig and chicken. We highlighted our new capability to perform high throughput systematic functional screens in livestock species. Potential collaborations and sharing of resources were discussed and are currently being acted on.
Year(s) Of Engagement Activity 2018
 
Description BBSRC Animal Health Research Club Fourth Dissemination Event 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact A retrospective report on the BBSRC Animal Health Research Club project on "Engineering resistance to Porcine Respiratory Syndrome virus (PRRSV)" was presented including the ongoing research in the BBSRC project " Understanding the CD163 - PRRS virus interaction to improve genetic engineering for resistanc" (BB/R004463/1) and outreach activities. This was followed by questions and discussions from/with scientists engaged in other BBSRC Animal Health Research Club and with representatives from the industrial co-funders of the BBSRC Animal Health Research Club.
Year(s) Of Engagement Activity 2018
 
Description British Science Festival Hull 2018: "From lab to farmyard: genome editing our livestock" 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact A talk on genome editing and it's potential impact on livestock breeding was presented to the general public at the British Science Festival in Hull in 2018. Around 50-100 people attended the talk. Publicity around the event, including blogging, press releases etc. reached a much larger public.
Year(s) Of Engagement Activity 2018
URL https://www.britishsciencefestival.org/event/from-lab-to-farmyard-genome-editing-our-livestock/
 
Description CPD event for High School Biology teachers 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Schools
Results and Impact SSERC CPD Summer School for 22 high school teachers. A lecture on the principles and application of genome editing and delivery of parts of a workshop we were developing on "Cows, Coughs and CRISPR". The participants reported an increased understanding of the topic and their feedback on the workshop allowed us identify an appropriate level of content and to further tailor it to the needs of the school curriculum.
Year(s) Of Engagement Activity 2018
 
Description Cabaret of Dangerous Ideas: Dolly the Sheep: major discovery or minor distraction? Presenter, Edinburgh Festival Fringe. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact Presentation with two colleagues, one a stem cell biologist and one a histrian of science in society, discussing the impact on research and society of the birth of Dolly the Sheep 20 years ago. Potential of GM technologies to confer resistance to diseases in farmed animals was discussed. Plenty of opportunity for audience contributions to debate.
Year(s) Of Engagement Activity 2016
URL http://www.youtube.com/watch?v=LClmL5C9YJE&feature=youtu.be
 
Description Career event at Earlston High 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Participated in school careers event at Earlston Highschool for pupils of S1.
Participated as STEM ambassador, multiple talks on career as scientist including activity People like me set up by STEM.
Year(s) Of Engagement Activity 2016
 
Description Come Dine With The Future 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact What will we be eating in 50 years time? H Sang and 4 other presenters described what will be on their dinner menu, considering advances in technology and challenges of sustainability
Year(s) Of Engagement Activity 2016
URL http://www.youtube.com/watch?v=Bkta-s7z9DY
 
Description Easter Bush Campus annual open day 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact The annual Roslin Institute Open Doors day was held on 13 October 2018 and was attended by over 600 people. It showcased a wide range of research arising from BBSRC strategic investment, including (but not limited to), gene editing food safety, influenza, genetic improvement, DNA, imaging and the ethical treatment of animals in research.
Year(s) Of Engagement Activity 2018
 
Description Edinburgh Science Festival, Flu Fighters 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Participated in Flu Fighters during the Edinburgh Science Festival 2015.
We showcased the exhibit 'Flu Fighters' at the festival. The 'Flu Fighters' exhibit focuses on influenza viruses and the science behind the varied approaches to combating flu being developed at Roslin and Pirbright. It showcases current research including GM animals, novel approaches to vaccines and diagnostic tests and host/virus genetics.
The exhibit also demonstrates how flu transmits from animals to humans and highlight the challenges of preventing large-scale outbreaks.The 'Flu Fighters' exhibit provides an opportunity for visitors to run diagnostic tests on a chicken, design their own flu virus and discuss the pros and cons of genetic modification of animals.
Over 100 people attended the morning and the activities sparked questions from both children and parents/carers. A better understanding of the problem for the poultry industry, eco system (wild birds) and human health was reported back.
Year(s) Of Engagement Activity 2015
 
Description Flu Fighters public exhibition stand 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact 4. With £10k BBSRC funding, ISPG4-funded researchers jointly developed 'Flu-Fighters', an interactive exhibit suitable for children and adults highlighting our research to create influenza-resistant farm animals, develop improved vaccines and diagnostic tests, and understand the role of viral and host factors in the outcome of infection. This was run over three days in November 2014 at the Great British Bioscience Festival (visited by over 6500 people) as part of BBSRC's 20th anniversary programme. ISPG4 scientists were involved in running it again at the Royal Society's 2015 Summer Science Exhibition in London.
Year(s) Of Engagement Activity 2014,2015,2016
 
Description GENOME 10K & GENOME SCIENCE Invited Talk 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Presentation by Alan Archibald at the NORWICH RESEARCH PARK, NORWICH, UK on "Precision engineering for PRRSV resistance in pigs".
Year(s) Of Engagement Activity 2017
 
Description Genome Editing and the Future of Farming 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Policymakers/politicians
Results and Impact On the 6th September The Roslin Institute opened its doors to an array of delegates from the worlds of research, industry and policy to discuss genome editing and its role in the future of farming, with many international colleagues including from USDA and OECD.
The global challenge of food security is time critical as we will need to produce 70% more food by 2050 without destroying the environment. Innovations in food production techniques are urgently required. Editing the genomes of crops and livestock offers new possibilities to address this complex issue. The meeting discussed the status of the technologies in crops and farmed animals and the associated regualtory and societal challenges were debated, lead by presenters with a broad array of relevant expertise. My role was as chair of a session.
Year(s) Of Engagement Activity 2016
URL http://www.nib.ac.uk/reporting-on-the-first-nib-specialist-meeting-genome-editing-and-the-future-of-...
 
Description Institute of Animal Pathogology, University of Bern. "Bringing high throughput approaches to livestock species". Invited talk. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Was invited to Bern to present on our work on developing CRISPR/Cas9 and ISG libraries for pig and chicken. Collaborations and sharing of resources were discussed and are being put into action. Additionally the visit was organised to increase links with an institute with aligned scientific interests.
Year(s) Of Engagement Activity 2018
 
Description International PRRSV symposium - South Korea 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact 60-100 practitioners met to learn about advances in PRRSV including control and diagnostics.
Year(s) Of Engagement Activity 2018
 
Description Invited Talk at the Wellcome Animal Genetics and Diseases conference 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact As part of the Wellcome Animal Genetics and Diseases conference I presented our and other peoples work on genome editing for disease resistance and production traits in livestock. The audience was a conglomerate of national and international scientists at all career stages and highly engaged in debating both scientific as well as policy questions surrounding genome editing technology.
Year(s) Of Engagement Activity 2019
 
Description Invited lecture at MSD-Animal Health 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact Invited lecture at MSD-Animal Health in Boxmeer the Netherlands, with WebEx connection to all other location o MSD -AH around the world.
Year(s) Of Engagement Activity 2019
 
Description Invited round table lead World Vaccine Congress 2018, Lisbon 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact In a session looking out to new developments in the world-wide vaccine sector I was asked to lead a round table on genome editing (for disease resistance) in livestock and how that may affect the sector but also whether the biopharma industry should invest in genome editing.
Year(s) Of Engagement Activity 2018
 
Description Invited speaker Heinrich Pette Institute 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Talk about using genome editing in livestock for disease resistance and why understanding protein interactions is essential to this. The audience were primarily staff and students of the HPI and the university of Hamburg. The institute is highly engaged in structural interaction studies and there was a fruitful discussion on how interdisciplinary collaborations can contribute to the larger picture.
Year(s) Of Engagement Activity 2019
 
Description Invited speaker Production Diseases in Farm Animals, 2019 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact Around 200 people attended my talk at the Production Diseases in Farm Animals conference. I presented on genome editing for disease resistance in livestock species, implications on breeding, and the regulatory framework and policies. This sparked a wide and interesting discussion with many follow-up questions primarily from members of industry.
Year(s) Of Engagement Activity 2019
 
Description Invited speaker summer school Leiden University 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Undergraduate students
Results and Impact Lecture on genome editing in livestock for production traits and disease resistance, the technology behind it, and the policy changes happening around the world. Around 25 students (both under- and postgraduate) attended the lecture and were engaged in a very broad discussion.
Year(s) Of Engagement Activity 2019
 
Description Invited talk European Veterinary Vaccinology Workshop 2018 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Talk on career opportunities in academia for young scientists
Year(s) Of Engagement Activity 2018
URL https://www.youtube.com/watch?v=hTujbImUCcI
 
Description Invited talk FU Berlin - Genome editing in livestock 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Presentation on genome editing in livestock in general (and the opportunities that creates) as well as specifics on genome editing in pigs for disease resistance. Both fellow scholars from different universities in Berlin as well as undergraduate and postgraduate students were attending the talk.
Year(s) Of Engagement Activity 2018
 
Description Invited talk on Enabling Collaboration in Livestock Research. BBSRC Session. BSAS Annual Conference 2018 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Presentation at the annual conference of the British Society of animal Science 2018 on "Gene edited pigs are resistant to PRRSV infection whilst maintaining biological function of the editing target gene CD163"
Year(s) Of Engagement Activity 2018
 
Description Keynote Lecture IPRRSS / IPVS 2018 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Presentation on "Gene edited pigs are resistant to PRRSV infection whilst maintaining biological function of the editing target gene CD163" at the international PRRSV symposium and the International Pig Veterinary Society meeting 2018 in Chonqing, China.
Year(s) Of Engagement Activity 2018
 
Description Laboratory based workshop for High school students 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Schools
Results and Impact As part of the funding of BB/P003966/1 we developed a hands on , full day, workshop, cows, coughs and CRIPSR, for High school pupils based on genome editing. This approach aligned with the Biology curriculum and was designed to a) give pupils hands on experience of common laboratory techniques, b) to introduce them to scientific method and the approach to developing a research project, c) to introduce them to the work done in the Institute and in this grant in particular and d) to increase there understanding of the life of a scientist and the opportunities available to them. 16 Pupils and3 staff from schools in North Lanarkshire , all from areas of social deprivation, attended the course. The pupils enjoyed the course and all felt that they had gained a better understanding of the science we do and of the potential careers open to them. Likewise the staff were very positive about the workshop and the effect on their pupils.
Year(s) Of Engagement Activity 2018
 
Description Membership of a guidance committee on PCV2 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact Expert Group on PCV2 for DISCONTOOLS FP 7 project, funded by DG Research
Year(s) Of Engagement Activity 2006,2008,2009,2011,2013,2015,2017,2019
 
Description Mercks Digital Journal 
Form Of Engagement Activity A magazine, newsletter or online publication
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Online publication: De Castro AMMG, Opriessnig T. PCV2 genotypes and cross protection. Mercks digital magazine. 2019
Year(s) Of Engagement Activity 2019
 
Description One Health Models of Infectious Disease. University of Edinburgh. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact Presenting our work on generation of genome wide CRISPR Cas9 libraries for pig and chicken. The meeting was aimed at establishing a Wellcome Trust studentship program at the Roslin Institute.
Year(s) Of Engagement Activity 2018
 
Description Presentation to the BBSRC executive committee "Bringing high throughput approaches to livestock species" 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Policymakers/politicians
Results and Impact The BBSRC executive were visiting Roslin Institute to review on going research programs as part of the ISP funding.
Year(s) Of Engagement Activity 2018
 
Description Press interviews on PRRSV-resistant pigs press release 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact In connection with the publication of our research paper "Pigs Lacking the Scavenger Receptor Cysteine-Rich Domain 5 of CD163 Are Resistant to Porcine Reproductive and Respiratory Syndrome Virus 1 Infection" (doi: 10.1128/JVI.00415-18) numerous press interviews were held and inquiries from the public responded to.

Media broadcast interviews (Radio or TV) UK:
• BBC Scotland
• BBC National
• BBC International
• BBC Farming Today
• BBC Sunday Morning Live

Print media interviews UK:
• The Guardian
• The Times
• Veterinary Times
• Vet Record
• The Scientist

International print and broadcast interviews:
• Swiss Radio & TV (SRF)
• NTN24 (CST Science, Health & Technology Magazine, Latin Americas)
• Country Today (Victoria, Australia, Radio)
• Country Life (Agricultural Newspaper, BC, Canada)
• Agri-Pulse (Agri Newspaper, Washington DC, USA)
• Bloomberg / Newsroom (Hong Kong)
• Rabobank / Raboworld (The Netherlands / Worldwide, Finance)

Requests from the general public / public organisations:
• Quality Meat Scotland
Year(s) Of Engagement Activity 2018,2019
 
Description Press releases JV IBV vaccine 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Press release was written after our publication in Journal of Virology on novel IBV vaccines. The press release was picked up and published by multiple magazines in the veterinary and agriculture field including:
front page with photo; Vet Times - https://www.vettimes.co.uk/news/vaccine-shows-promise-against-chicken-disease/
Farming UK - https://www.farminguk.com/news/New-vaccine-could-help-fight-against-major-poultry-disease_50541.html
Agriland - https://www.agriland.co.uk/farming-news/vaccine-shows-promise-against-costly-poultry-disease/
Stackyard - http://www.stackyard.com/news/2018/10/vet/06_edinburgh_vaccine.html
Global Meat News - https://www.globalmeatnews.com/Article/2018/10/23/Chicken-disease-vaccine-progress
Technology Networks - https://www.technologynetworks.com/tn/news/vaccine-shows-promise-against-chicken-disease-310951
Farming Sector - https://www.farmingsector.co.uk/latest-news/livestock/new-vaccine-could-protect-poultry-from-serious-disease/
Medical Xpress- https://medicalxpress.com/news/2018-10-vaccine-widespread-chicken-disease.html
News Medical - https://www.news-medical.net/news/20181023/New-vaccine-strategy-shows-promise-to-protect-chickens-against-serious-respiratory-disease.aspx


Feed stuff - https://www.feedstuffs.com/nutrition-health/vaccine-shows-promise-against-ibv

Science Daily - https://www.sciencedaily.com/releases/2018/10/181023110540.htm

Genetic Literacy Project - https://geneticliteracyproject.org/2018/10/25/bronchitis-in-chickens-can-cut-egg-production-70-new-vaccine-may-stem-outbreaks/
Year(s) Of Engagement Activity 2018
 
Description Press reports on PRRSV-resistant pigs 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact In connection with the publication of our research paper "Pigs Lacking the Scavenger Receptor Cysteine-Rich Domain 5 of CD163 Are Resistant to Porcine Reproductive and Respiratory Syndrome Virus 1 Infection" (doi: 10.1128/JVI.00415-18) we published a press package and answered interview request from many broadcasting and media agencies. As a result the following print and broadcast pieces were released:

International Broadcast:
BBC Scotland, BBC News at Six, BBC News at Ten, BBC World, BBC Sunday Morning Live, BBC Radio 4 Farming Today, BBC Radio Five Live, BBC Radio 4, BBC Radio Scotland, BBC World Service, Guardian Podcast, BBC Radio Shetland, Swiss Radio & TV (SRF), Country Today (Victoria, Australia, Radio), Fiji Broadcasting Corporation, France 24

Print UK:
The Daily Telegraph, The Times, The Guardian, Scottish Daily Mail, The i, Scottish Daily Express, The Sun, Scottish Sun, Daily Mirror, Daily Star, Metro, The Herald, Scotsman, Daily Record, Dundee Courier, Edinburgh Evening News, Aberdeen Evening Express, The Week, Press & Journal, Yorkshire Post

Online UK
BBC Online, Daily Telegraph, Daily Mail, The Times, The Guardian, Daily Mirror, Daily Express, Scotsman, Irish News, Irish Examiner, Shropshire Star, Express & Star, Hastings Observer, Scarborough News, Eastbourne Herald, Shoreham Herald, West Sussex County Times, Yorkshire Evening Post, Glasgow South & Eastwood Extra, Rye & Battle Observer, Crawley Observer, Bognor Regis Observer, Mid Sussex Times, Worthing Herald, Littlehampton Gazette, Bexhill-on-Sea Observer, Portsmouth News, Chichester Observer, Midhurst & Petworth Observer, Dundee Evening Telegraph, Aberdeen Evening Express, Independent Recorder, TDNews, BT.com, BreakingNews.ie, The London Economic, The Weekly Observer, The Scientist, The London Economic, Science Magazine, Earth.Com, Independent Recorder, BT.com, Breakingnews.IE, I4U News, Press & Journal, Livekindly.co, Tech Times, IFLScience, Technology Networks

Online / Print International:
NTN24 (CST Science, Health & Technology Magazine, Latin Americas), Infosurhoy, The Economic Times (India), First Post (India), Eurasia Review, HealthEuropa, Jstor Daily, Times Of India, St Lucia News Online, Times Now (India), Business Standard (India), CanIndia.com (India), Green Report (Italy), New Kerala.com, Fanpage.It (Italy), Le Monde Veterinaire (France)

Veterinary / Science-related press:
Agri-Pulse, Veterinary Times, Vet Record, Pig World, Feedstuffs, Laboratory Equipment, GlobalMeatNews.com, Animal Pharm, Ag Daily, Agribusiness Intelligence, Laboratory Equipment, Pig Progress, Pig World, Labiotech.EU, MRCVS, Farmweek, National Hog Farmer, Farming UK, Frontline Genomics, Farmers Weekly, Food Weekly News
Year(s) Of Engagement Activity 2018,2019
URL https://www.ed.ac.uk/roslin/news-events/latest-news/gene-edited-pigs-resistant-billion-dollar-virus
 
Description Regulation of splicing by the host ubiquitin segregase, VCP/p97, is essential for human cytomegalovirus replication 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact invited seminar at Glasgow Virology Workshop, University of Glasgow .
Year(s) Of Engagement Activity 2016
 
Description Roslin Institute and R(D)SVS stand at Royal Highland Show, Edinburgh, Scotland 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Stand at the Royal Highland Show with exhibits covering different aspects of research including avian influenza, animal breeding and sheep transcriptomics.
Year(s) Of Engagement Activity 2013,2014,2016,2017
 
Description Science in the Saddle 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact A guided bike ride around Midlothian county, interspersed with stops and short talks by scientists, usually connected with sites of local scientific interest.

Sadly the event had to be cancelled on the day because of awful weather, but we will run it again in 2019
Year(s) Of Engagement Activity 2018
URL https://midlothiansciencefestival.com/event/science-in-the-saddle-3/
 
Description Seminar on genome editing for PRRSV resistance at CLSU, Munoz, The Philippines 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Undergraduate students
Results and Impact Genome editing for disease resistance in livestock in general and in pigs for PRRSV resistance was presented to all veterinary students and other department students at Central Luzon State University in the Philippines. Students could inquire the techniques and developments in this field and assess identify new approaches to disease treatments / prevention in animals.
Year(s) Of Engagement Activity 2019
 
Description Swine Practitioner workship - Vietnam 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact 150 practitioners attended a workshop to learn about new strategies on virus control in pigs.
Year(s) Of Engagement Activity 2018
 
Description Swine Practitioner workshop 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Practitioner meeting in France to inform them on new insights on vaccination protocols.
Year(s) Of Engagement Activity 2017
 
Description Swine Practitioner workshop - Brazil 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact 200 pig vets attended a workshop on updates on current pig diseases.
Year(s) Of Engagement Activity 2018
 
Description Swine Practitioner workshop - China 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Professional Practitioners
Results and Impact Practitioners met to learn about advances in PCV2 research including control and diagnostics.
Year(s) Of Engagement Activity 2018
 
Description Swine Practitioner workshop - France 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Practitioners discussed the pro and cons of control of swine diseases in France.
Year(s) Of Engagement Activity 2019
 
Description Swine Practitioner workshop - France 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact Key opinion leaders from Asia were invited to discuss advances in PCV2 research including control and diagnostics.
Year(s) Of Engagement Activity 2018
 
Description Swine Practitioner workshop - Germany 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact 3--40 veterinarians/industry representatives discussed other approached to diagnose and treat infectious disease in pigs.
Year(s) Of Engagement Activity 2019
 
Description Swine Practitioner workshop - Italy 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Professional Practitioners
Results and Impact 50-60 practitioners discussed other approached to diagnose and treat infectious disease in pigs.
Year(s) Of Engagement Activity 2019
 
Description Swine Practitioner workshop - Korea 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact We discussed novel approaches to diagnose and treat infectious disease in pigs. We also discussed to make a workshop in China which will occur in November 2020
Year(s) Of Engagement Activity 2019
 
Description TedX Glasgow "Why do we need GM chickens?" 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Other audiences
Results and Impact TedX Glasgow is a volunteer-run event attended by a an audience from school children to people supported by their employers to attend for personal development. The talks are very varied addressing a wide range of topics and so this was an opportunity to talk about GM technology in a Scottish context and in the context of sustainable agriculture. There was an oportunity at the end of the day to meet members of the audience freely and I had a lot of interesting discussions. The Youtube video has ~1390 views. https://www.youtube.com/watch?v=DUbqrh5otWs
Year(s) Of Engagement Activity 2017
URL https://www.youtube.com/watch?v=DUbqrh5otWs
 
Description Transgenic Animal Research Conference XI 2017 - Invited talk 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Talk at the Transgenic Animal Research Conference XI 2017 at Lake Tahoe, CA, USA on "Precision engineering for PRRSV resistance in pigs: Macrophages from pigs lacking CD163 SRCR domain 5 are PRRSV resistant"
Year(s) Of Engagement Activity 2017
 
Description dissecting the interferon response to human cytomegalovirus 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Professional Practitioners
Results and Impact Invited seminar to the Edinburgh infectious disease symposium.
Year(s) Of Engagement Activity 2018