Food Innovation

Lead Research Organisation: Quadram Institute Bioscience
Department Name: Contracts

Abstract

The majority of dietary advice informing policy for the maintenance and promotion of health is based upon observational studies that associate estimations of dietary intake with the occurrence of chronic disease. Observational studies are useful, but cannot provide mechanistic insight of how foods and diets can promote health. Through understanding the underlying mechanisms, the beneficial functional traits of foods can be enhanced. However, it is most challenging to disassociate functional components of individual foods and complex diets from confounding life-style factors, and to design of well controlled human intervention studies with complex foods.

To address this challenge, Theme 1 ‘Food Innovation’ will focus on developing innovative foods that have contrasting chemical and physical properties that may affect health related indices including rate of digestion, bioavailability of nutrients and non-nutrient bioactives, gut hormone signalling and microbial communities in the upper and lower GI tract. These foods will be used to test hypotheses in both in vitro and in vivo studies in Themes 2, 3, and 4.

This will involve exploiting recent advances in crop genomics including sequenced wheat TILLING populations. Selected crop genotypes with variation in starch biosynthetic genes will be used to explore the effects on digestion of carbohydrates. A complementary approach to delay starch digestion is through food processing and to retain starch (and other macronutrients) within plant organelles in the food product. Experimental foods of this nature will be developed with an emphasis on exploiting pulses to retain plant structures through innovative processing procedures.

We will also seek to develop novel food products that are rich in specific plant specialised metabolites that have been implicated in the control of lipid and carbohydrate metabolism or which would be expected to beneficially alter gut microbiota metabolism. This will include using new genetic technologies, including gene editing in Brassica to understand how specialised sulphur metabolism is regulated. New food products will be developed for use in well controlled human intervention studies.

The outputs of this work will deliver insights into the genetic regulation of nutritional traits in plants, and how these can be translated into food products which may impact health. Secondly, it will generate experimental material for Themes 2, 3 and 4. It will also facilitate collaboration with the commercial food sector leading to the development of new food products with enhanced nutritional attributes.

Publications

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publication icon
Birsan RI (2019) Recovery of Polyphenols from Brewer's Spent Grains. in Antioxidants (Basel, Switzerland)

 
Title Hungry Bob 
Description An interactive and educational model of the human digestive tract 
Type Of Art Artwork 
Year Produced 2019 
Impact Hungry Bob was used in public engagement activities in association with the Norwich Science Festival 
 
Title PulseON logo 
Description Graphical design logo for PulseON 
Type Of Art Artwork 
Year Produced 2019 
Impact Has been used on product concepts and engagement with industry and the public to support communication of the inrgedienmt concept. Consumer feedback was positive and many reported that to them the artwork symbolized health, beans and energy. 
 
Description • We have made progress in the development of new wheat starche TILLING mutants. We have completed an initial characterization of starch and grain properties of Cadenza ssIIIa single, double and triple mutants and are currently preparing a manuscript describing their development and characterization of starch and grain properties (starch content, amylose content, resistant starch content, protein content, starch chain length distribution, starch granule size distribution, starch granule morphology, germination index, grain hardness, grain weight, grain dimensions, physicochemical properties). We showed that loss of SSIIIa in triple mutants lead to more amylose and resistant starch, and our results indicate that despite the higher levels of resistant starch, other effects on starch and grain properties may hamper the usefulness of ssIIIa mutants in breeding applications (obj. 1.1)
• We have made progress in developing a high throughput method to assess starch digestibility in wheat flour samples (Obj. 1.1).
• We used sbeII durum and common wheat mutants to produce two wheat-based food products (semolina pudding and bread rolls) to determine the resistant starch and starch digestibility of the foods (Obj1.2).
• We have analysed how increases in amylose and resistant starch in durum wheat, due to mutation in SBEII genes, affect the glycaemic index in a randomised controlled pilot study (Obj. 1.2).
• Leguminous food ingredients with contrasting structures and products (e.g., various baked goods, biscuits) incorporating these ingredients have been produced and characterized by the Edwards Group. Ingredients prepared from chickpeas, peas, beans, lentils all retain high levels of plant cell integrity and type 1 resistant (Edwards et al 2020a and 2020b) but flours from the same botanical source are highly digestible once processed. The resilience of cell structures to secondary processing conditions used in the bakery sector has been confirmed, and IAA-funded activities have enabled development of product concepts and prototypes. Chickpea cell powders were incorporated into white wheat bread at high doses without adverse effects of on product quality (Bajka et al 2021). The data collected thus far indicates that some forms of processing has a detrimental effect on type 1 resistant starch, and work is underway to improve understanding of how different processing treatments on these structures. These findings highlight the importance of intact plant cells as key dietary fibre structures found in legumes that likely underpin nutritional and health benefits associated with pulses in some processed foods (Obj. 1.3).
• Work has been progressing in several areas. One model system of interest is the high resistant starch (RS) rr variant pea, compared to the wild type RR pea. Starch analysis shows that the rr and RR peas react differently to processing in terms of RS. We believe that this is a function of differences in the starch granule microstructure between the two types. In addition, pleiotropic effects of the mutation result in small increases in protein, and dietary fibre (cell wall material). This is thought to be responsible for increased hardness in the rr pea, which results in a greater particle size following simulated chewing. This in turn will reduce the availability of starch during digestion (work combined from this and the pea DRINC project). A manuscript has been published in Nature Foods (https://doi.org/10.1038/s43016-020-00159-8) . Workshops were held to develop follow on funding with industry in collaboration with Imperial College and John Innes Centre based on the results from this and the pea DRINC project, a super follow on fund proposal was submitted in October 2020 (Obj. 1.3).
• Other work is focussing on the forms of dietary fibre and their interaction with lipids and bile salts. Human studies have shown conflicting results of the impact of B-glucan on serum cholesterol. Much of this variance is probably due to the poor characterisation of materials used in these studies. We have shown that certain types of fibre preparation either binds or entraps bile salts in the food matrix, and that this is very sensitive to processing. In contrast, B-glucan alone does not show any interaction with bile. However, there does appear to be a synergy between the form of the fibre, incubation time and viscosity which may implicate a role of B-glucans if presented in the appropriate matrix. Further progress indicates that the molecular weight and viscosity of the beta-glucan fraction is important for driving this synergistic interaction between the soluble and insoluble fibre fractions (see theme 2). A BBSRC response mode application will be submitted in April 2021 and a manuscript is in preparation. Also, collaborative work with the University of Warmia and Mazury, Poland has shown a novel interaction between beta-glucans and milk proteins, and that the source of the beta glucan is critical (cereal, fungal or bacterial). This work has been published in Food Hydrocolloids (https://doi.org/10.1016/j.foodhyd.2020.106486) (Obj. 1.3).
• Dietary inhibitors of TMA production in the gut: We have shown that human faecal material can efficiently convert choline to trimethylamine in an in vitro model of the human colon, and that this is microbiota-dependent. More recently we have also shown that this model is suitable for studying the conversion of the alternative TMA substrates betaine, carnitine and ?-butyrobetaine to TMA (manuscript submitted). We have tested several isolated polyphenols, enriched extracts and dietary fibres for their ability to inhibit the production of the proatherogenic metabolite TMA from choline and identified two effective treatments which will now be studied in more detail (e.g. dose-response relationship, mechanism(s) of action). Work to expand this research to include probiotics is going on in parallel supported by a multinational commercial partner in a CASE studentship. We have designed a dietary intervention proof-of-principle study to test in humans the effect of a polyphenol that we have shown to be an effective inhibitor of TMA production in vitro, and the ethics paperwork is being completed (Obj. 1.4).
• Modifying sulphur-containing specialised plant metabolites in foods: We have explored the effect of thermal processing on the stability of S-containing bioactive compounds in processed food products. We have shown that glucosinolates are not thermally stable above 110 degrees C and are degraded, whereas S-allyl cysteine sulfoxides are stable. This has implication for the development of nutritious processed food products. Further studies will explore the possible us of high-pressure processing (Obj. 1.5).
• Whole genome sequencing, RNA expression and metabolite analyses were used to characterise the consequences of the introgression of either one or two copies of a genetic variant of the MYB28 transcription factor from a wild Brassica species into a commercial broccoli genetic background. The introgression of the variant of MYB28 resulted in enhanced expression of broccoli genes involved in primary sulphate assimilation, sulphur metabolism and aliphatic glucosinolate biosynthesis, and enhanced accumulation of 4-methylsulphinyl butyl glucosinolate in florets. Other changes in transcription that may be related to non-targeted introgression events were reported. There were no effects upon sulphur metabolites pools apart from methionine-derived glucosinolates (Obj. 1.5).
Exploitation Route The understanding gained from structure-function studies will underpin translational research which aims to preserve resistant starch in food products. The data from structure-function studies will also inform the routes-to-market for novel food ingredients (P10357GBWO, patent pending). Edwards is engaged in confidential discussions with selected industry members who have potential to implement findings on a commercial level. The work complements commercially-driven project BBSRC Super Follow On (Edwards), and 2 x Impact Acceleration Account awards have been awarded to support commercialization.
• New understanding of processing effects on dietary fibre structure and its nutritional implications raises new questions about analysis methods and definitions of dietary fibre and starch in a food, which is relevant for food labelling regulations and food composition data.
• We have engaged with a global food and ingredients company who are interested in developing and eventually commercialising a TMAO-lowering food product. They have agreed to support a CASE studentship with Kroon as PI/primary supervisor.
• The understanding of starch digestibility in sbeII wheat-based food products was used to design a test food for a dietary intervention study exploring the effect of resistant starch on postprandial glycaemic response.
• A super-follow on fund application was submitted to translate the findings of this and the pea DRINC project (In collaboration with Imperial College and John Innes Centre), towards the development of foods and ingredients to tackle/prevent type 2 diabetes. Knowledge regarding the relationships between soluble and insoluble fibres and how they will interact with bile salts and other digestive processes will aid the design of commonly consumed foods to increase the effectiveness of the fibre content on health, whilst maintaining the quality and consumer acceptability of such foods.
Sectors Agriculture, Food and Drink,Healthcare

 
Description TransformingNutrition Science for Better Health - A joint Wellcome -WHO consultation
Geographic Reach Multiple continents/international 
Policy Influence Type Participation in a national consultation
 
Description BBSRC IAA
Amount £10,000 (GBP)
Funding ID BB/S506679/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 01/2019 
End 04/2019
 
Description BBSRC IAA The Quadram Institute
Amount £300,000 (GBP)
Funding ID BB/S506679/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 03/2018 
End 03/2021
 
Description Impact on sustainability, shelf-life and nutritional value by vertically farmed leafy greens via a new local supply chain
Amount € 729,415 (EUR)
Organisation European Union 
Sector Public
Country European Union (EU)
Start 01/2020 
End 12/2020
 
Description QIB PoC Fund
Amount £9,278 (GBP)
Organisation Quadram Institute Bioscience 
Sector Academic/University
Country United Kingdom
Start 03/2019 
End 06/2019
 
Description Alan Mackie - University of Leeds 
Organisation University of Leeds
Country United Kingdom 
Sector Academic/University 
PI Contribution Jointly supervised PhD studentship and joint contributions to papers
Collaborator Contribution Jointly supervised PhD studentship and joint contributions to papers
Impact Mulet-Cabero, A. I., A. R. Mackie, P. J. Wilde, M. A. Fenelon and A. Brodkorb (2019). "Structural mechanism and kinetics of in vitro gastric digestion are affected by process-induced changes in bovine milk." Food Hydrocolloids 86: 172-183. Ana-Isabel Mulet-Cabero: PhD student completed successfully, University of East Anglia, December 2018. Thesis title: Effect of Dairy Structures on Gastric Behaviour and Nutrient Digestion Kinetics using a Semi-Dynamic Model
Start Year 2017
 
Description Bespoke synthesis of polyphenol human metabolites 
Organisation University of Barcelona
Country Spain 
Sector Academic/University 
PI Contribution We used synthetic organic chemistry routes to make phase-2 conjugates of various dietary flavonoids and phenolic acids and supplied these to various collaborators for testing of biological activity. These compounds are not available commercially and for many of them they are unique to our lab.
Collaborator Contribution Partners have used these physiologically relevant metabolites to test their biological activity using in vitro models such as cultured mammalian cell models, etc... They have also used them as authentic standards to facilitate identification and quantification of metabolites in human and animal tissues including blood, urine and faeces.
Impact Petri N, Tannergen C, Holst B, Mellon FA, Bao Y, Plumb GW, Bacon J, O'Leary KA, Kroon PA, Knutson L, Forsell P, Eriksson T, Lennernas H & Williamson G (2003) "Absorption/metabolism of sulforaphane and quercetin, and regulation of phase II enzymes, in human jejenum in vivo". Drug Metab Disposition 31, 805-813.Nemeth K, Plumb GW, Berrin J-G, Juge N, Jacob R, Naim HY, Williamson G, Swallow DM, Kroon PA (2003) "Deglycosylation by small intestinal epithelial cell ? glucosidases is a critical step in the absorption and metabolism of dietary flavonoid glycosides in humans. Eur J Nutr 42, 29-42.Kroon PA, Clifford MN, Crozier A, Day AJ, Donovan JL, Manach C & Williamson G (2004). How should we assess the effects of exposure to dietary polyphenols in vitro? Am J Clin Nutr 80, 15-21. Dupont MS, Day AJ, Bennett RN, Mellon FA & Kroon PA (2004) "Absorption of kaempferol-3-glucuronide from endive in humans". Eur J Clin Nutr 58, 947-954. Needs PW, Kroon PA (2006) Convenient syntheses of metabolically important quercetin glucuronides and sulfates. Tetrahedron 62, 6862-6868. Davis BD, Needs PW, Kroon PA & Brodbelt (2006) Identification of isomeric flavonoid glucuronides in urine and plasma by metal complexation and liquid chromatography/tandem mass spectrometry. J Mass Spectrom 41, 911-920. Tribolo S, Lodi F, Connor C, Suri S, Wilson V, Taylor M, Needs PW, Kroon PA, Hughes DA (2008). Comparative effects of quercetin and its predominant human metabolites on adhesion molecule expression in activated human vascular endothelial cells. Atherosclerosis 197, 50-56. Loke WM, Proudfoot JM, Stewart S, McKinley AJ, Needs PW, Kroon PA, Hodgson JM & Croft KD (2008). Metabolic transformation has a profound effect on anti-inflammatory activity of flavonoids such as quercetin: Lack of association between antioxidant and lipoxygenase inhibitory activity. Biochem Pharmacol 75, 1045-1053. Loke WM, Proudfoot JM, Stewart S, McKinley AJ, Needs PW, Kroon PA, Hodgson JM & Croft KD (2008). Quercetin and its in vivo metabolites inhibit neutrophil-mediated low-density lipoprotein oxidation. J Agric Food Chem 56, 3609-3615. Hollands W, Brett GM, Radreau P, Saha S, Teucher B, Bennett, RN & Kroon PA (2008). Processing blackcurrants dramatically reduces the content and does not enhance the urinary yield of anthocyanins in human subjects. Food Chem 108, 869-878. Suri S, Taylor MA, Verity A, Tribolo S, Kroon PA, Hughes DA, Wilson VG (2008). A comparative study of the effects of quercetin and its glucuronide and sulphate metabolites on human neutrophil function in vitro. Biochem Pharmacol 76, 645-673. Barrington R, Williamson G, Bennett RN, Davis BD, Brodbelt JS, Kroon PA (2009). Absorption, conjugation and efflux of the flavonoids, kaempferol and galangin, using the intestinal CaCo-2/TC7 cell model. J Functional Foods 1, 74-97. Brett GM, Hollands W, Needs PW, Teucher B, Dainty JR, Bennett RN, Davis BD, Brodbelt JS & Kroon PA (2009). Absorption, metabolism and excretion of flavanones from single portions of orange fruit and juice and effects of anthropometric variables and contraceptive pill use on flavanone excretion. Br J Nutr 101, 664-675. http://journals.cambridge.org/repo_A44rP6ra (Personal author link) Winterbone MS, Tribolo S, Needs PW, Kroon PA & Hughes DA (2009). Physiologically relevant metabolites of quercetin have no effect on adhesion molecule or chemokine expression in human vascular smooth muscle cells. Atherosclerosis 202, 431-438. Kay CD, Kroon PA, Cassidy A (2009). The bioactivity of dietary anthocyanins is likely to be mediated by their degradation products. Mol Nutr Food Res 53 Suppl 1:S92-101. Lodi F, Jiménez R, Moreno L, Kroon PA, Needs PW, Hughes DA, Santos-Buelga C, González-Paramás A, Cogolludo A, Duarte J, Perez-Vizcaino F (2009). Glucuronidated and sulfated metabolites of the flavonoid quercetin prevent endothelial dysfunction but lack direct vasodilator effects in rat aorta. Atherosclerosis 204, 34-39. Radreau P, Rhodes JD, Mithen RF, Kroon PA, Sanderson J (2009) Hypoxia-inducible factor-1 (HIF-1) pathway activation by quercetin in human lens epithelial cells. Exp Eye Res 89, 995-1002. Curtis PJ, Kroon PA, Hollands WJ, Walls R, Jenkins G, Kay CD, Cassidy A (2009). Cardiovascular disease risk biomarkers and liver function are not altered following twelve week ingestion of an elderberry extract rich in anthocyanins. J Nutr 139, 2266-2271. Soler A, Romero MP, Saha S, Furniss CSM, Kroon PA, Motilva MJ (2010). Digestion stability and evaluation of the metabolism and transport of olive oil phenols in human small intestinal epithelial Caco2/TC7 cell line. Food Chem, 119, 703-714. Suri S, Liu XH, Rayment S, Hughes DA, Kroon PA, Needs PW, Taylor MA, Tribolo S, Wilson VG (2010). Quercetin and its major metabolites selectively modulate cyclic GMP-dependent relaxations and associated tolerance in pig isolated coronary artery. Br J Pharmacol 159, 566-575. Bartholomé R, Haenen G, Hollman PCH, Bast A, Dagnelie PC, Roos D, Keijer J, Kroon PA, Needs PW, Arts ICW (2010). Deconjugation kinetics of glucuronidated phase-II flavonoid metabolites by ß-glucuronidase from neutrophils. Drug Metab Pharmacokinet 25, 379-387. Pereira-Caro G, Mateos R, Saha S, Madrona A, Espartero JL, Bravo L, Kroon PA (2010). Trans-epithelial transport and metabolism of new lipopohilic ether derivatives of hydroxytyrosol by enterocyte-like CaCo-2/TC7 cells. J Agric Food Chem 58, 11501-11509. Al-Shalmani S, Suri S, Hughes DA, Kroon PA, Needs PW, Taylor MA, Tribolo S, Wilson VG (2011). Quercetin and its principal metabolites, but not myricetin, oppose LPS-induced hyporesponsiveness of the porcine isolated coronary artery. Br J Pharmacol 162, 1485-1497. Curtis PJ, Sampson M, Potter J, Dhatariya K, Kroon PA, Cassidy A (2012). Chronic ingestion of flavan-3-ols and isoflavones improves insulin sensitivity and lipoprotein status and attenuates estimated 10-year CVD risk in medicated postmenopausal women with type 2 diabetes: a one year double-blind randomized controlled trial. Diabetes Care 35, 226-232. Saha S, Hollands W, Needs PW, Ostertag LM, de Roos B, Duthie GG, Kroon PA (2012). Human O-sulfated metabolites of (-)-epicatechin and methyl-(-)-epicatechin are poor substrates for commercial aryl-sulfatases: Implications for studies concerned with quantifying epicatechin bioavailability. Pharmacol Res 65, 592-602. Lodi F, Tribolo S, Winterbone MS, Needs PW, Hughes DA, Kroon PA (2012). Human quercetin conjugated metabolites attenuate TNF-a-induced changes in vasomodulatory molecules in a HUASMCs/HUVECs co-culture model. Planta Med 78, 1571-1573. Hollands WJ, Hart D, Dainty JR, Hasselwander O, Tiihonen K, Wood R, Kroon PA (2013). Bioavailability of epicatechin and effects on nitric oxide metabolites of an apple flavanol-rich extract supplemented beverage compared to a whole apple puree: a randomized, placebo controlled, crossover trial. Mol Nutr Food Res 57, 1209-1217. Tribolo S, Lodi F, Winterbone MS, Saha S, Needs PW, Suri S, Taylor MA, Wilson VG, Walls R, Cassidy A, Hughes DA, Kroon PA (2013). Human metabolic transformation of quercetin blocks its capacity to decrease eNOS expression and endothelin-1 secretion by human endothelial cells. J Agric Food Chem 61, 8589-8596. Czank C, Cassidy A, Zhang Q, Morrison DJ, Preston T, Kroon PA, Botting NP, Kay CD (2013). Human metabolism and elimination of the anthocyanin, cyanidin-3-glucoside: a 13C-tracer study. Am J Clin Nutr 97, 995-1003. Konic-Ristic A, Srdic-Rajica T, Kardum N, Kroon PA, Hollands WJ, Hayran O, Boyko N, Jorjadze M, Glibetic M (2013). Effects of bioactive-rich extracts of pomegranate, persimmon, nettle, dill, kale and Sideritis and isolated bioactives on arachidonic acid induced markers of platelet activation and platelet-leucocyte aggregation. J Sci Food Agric 93, 3581-3587. De Ferrars RM, Czank C, Zhang Q, Botting NP, Kroon PA, Cassidy A, Kay CD (2014). The pharmacokinetics of anthocyanins and their metabolites in humans. Br J Pharmacol 171, 3268-3282. De Ferrars R, Czank C, Saha S, Needs PW, Zhang Q, Raheem KS, Kroon PA, Kay CD (2014). Methods for isolating, identifying and quantifying anthocyanin metabolites in clinical samples. Anal Chem 86, 10052-10058. Danesi F, Kroon PA, Saha S, de Biase D, D'Antuono LF, Bordoni A (2014). Mixed pro- and anti-oxidative effects of pomegranate polyphenols in cultured cells. Int J Mol Sci 15, 19458-19471. Barrington RD, Needs PW, Williamson G, Kroon PA. MK571 inhibits phase-2 conjugation of flavonols by Caco-2/TC7 cells, but does not specifically inhibit their apical efflux. Biochem Pharmacol 95, 193-200. Cerezo AB, Winterbone MS, Moyle CW, Needs PW, Kroon PA (2015). Molecular structure-function relationship of dietary polyphenols for inhibiting VEGF-induced VEGFR-2 activity. Mol Nutr Food Res. 59, 2119-2131. doi: 10.1002/mnfr.201500407. Gornas P, Redenkovs V, Pugacheva I, Soliven A, Needs PW, Kroon PA (2016). Varied composition of tocochromanols in different types of bran: Rye, wheat, oat, spelt, buckwheat, corn and rice. Int J Food Properties 19, 1757-1764. Dower JI, Geleijnse JM, Kroon PA, Philo M, Mensink M, Kromhout D, Hollman PCH (2016). Does epicatechin contribute to the acute vascular function effects of dark chocolate? A randomised, crossover study. Mol Nutr Food Res 60, 2379-2386. Poór M, Boda G, Needs PW, Kroon PA, Lemli B, Bencsik T (2017). Interaction of quercetin and its metabolites with warfarin: displacement of warfarin from serum albumin and inhibition of CYP2C9 enzyme. Biomed Pharmacol 88, 574-581. Hollands WJ*, Voorspoels S*, Jacobs G, Aaby K, Meisland A, Garcia-Villalbad R, Tomas-Barberan F, Piskula MJ, Mawson D, Vovk I, Needs PW and Kroon PA (2017). Development, validation and evaluation of an analytical method for the determination of monomeric and oligomeric procyanidins in apple extracts. J Chromatogr A 1495, 46-56. Van Rymenant E, Grootaert C, Beerens K, Needs P, Kroon P, Kerimi A, Williamson G, García Villalba R, González-Sarrías A, Tomas-Barberan F, Van Camp J, Van de Voorde J. Vasorelaxant activity of twenty-one physiologically relevant (poly)phenolic metabolites on isolated mouse arteries. Mol Nutr Food Res 13, 4331-4335. Perez-Moral N, Saha S, Philo M, Hart DJ, Winterbone MS, Hollands WJ, Spurr M, Bows J, vander Velpen V, Kroon PA*, Curtis PJC (2018) Comparative bio-accessibility, bioavailability and bioequivalence of quercetin, apigenin, glucoraphanin and carotenoids from freeze-dried vegetables incorporated into a baked snack versus minimally processed vegetables: Evidence from in vitro models and a human bioavailability study. J Func Food 48, 410-419. Poor M, Boda G, Kunsagi-Mate S, Needs PW, Kroon PA, Lemli B (2018). Fluorescence spectroscopic evaluation of the interactions of quercetin, isorhamnetin, and quercetin-3 '-sulfate with different albumins. J Luminescence 194, 156-163. Wu Q, Kroon PA, Shao HJ, Needs PW, Yang XB (2018). Differential effects of quercetin and two of its derivatives, isorhamnetin and isorhamnetin-3-glucuronide, in inhibiting the proliferation of human breast cancer MCF-7 cells. J Agric Food Chem 66, 7181-7189. Wu Q, Needs PW, Lu YL, Kroon PA, Ren DY, Yang XB (2018). Different antitumor effects of quercetin, quercetin-3'-sulfate and quercetin-3-glucuronide in human breast cancer MCF-7 cells. Food Func 9, 1736-1746.
Start Year 2010
 
Description Bespoke synthesis of polyphenol human metabolites 
Organisation University of Milan
Country Italy 
Sector Academic/University 
PI Contribution We used synthetic organic chemistry routes to make phase-2 conjugates of various dietary flavonoids and phenolic acids and supplied these to various collaborators for testing of biological activity. These compounds are not available commercially and for many of them they are unique to our lab.
Collaborator Contribution Partners have used these physiologically relevant metabolites to test their biological activity using in vitro models such as cultured mammalian cell models, etc... They have also used them as authentic standards to facilitate identification and quantification of metabolites in human and animal tissues including blood, urine and faeces.
Impact Petri N, Tannergen C, Holst B, Mellon FA, Bao Y, Plumb GW, Bacon J, O'Leary KA, Kroon PA, Knutson L, Forsell P, Eriksson T, Lennernas H & Williamson G (2003) "Absorption/metabolism of sulforaphane and quercetin, and regulation of phase II enzymes, in human jejenum in vivo". Drug Metab Disposition 31, 805-813.Nemeth K, Plumb GW, Berrin J-G, Juge N, Jacob R, Naim HY, Williamson G, Swallow DM, Kroon PA (2003) "Deglycosylation by small intestinal epithelial cell ? glucosidases is a critical step in the absorption and metabolism of dietary flavonoid glycosides in humans. Eur J Nutr 42, 29-42.Kroon PA, Clifford MN, Crozier A, Day AJ, Donovan JL, Manach C & Williamson G (2004). How should we assess the effects of exposure to dietary polyphenols in vitro? Am J Clin Nutr 80, 15-21. Dupont MS, Day AJ, Bennett RN, Mellon FA & Kroon PA (2004) "Absorption of kaempferol-3-glucuronide from endive in humans". Eur J Clin Nutr 58, 947-954. Needs PW, Kroon PA (2006) Convenient syntheses of metabolically important quercetin glucuronides and sulfates. Tetrahedron 62, 6862-6868. Davis BD, Needs PW, Kroon PA & Brodbelt (2006) Identification of isomeric flavonoid glucuronides in urine and plasma by metal complexation and liquid chromatography/tandem mass spectrometry. J Mass Spectrom 41, 911-920. Tribolo S, Lodi F, Connor C, Suri S, Wilson V, Taylor M, Needs PW, Kroon PA, Hughes DA (2008). Comparative effects of quercetin and its predominant human metabolites on adhesion molecule expression in activated human vascular endothelial cells. Atherosclerosis 197, 50-56. Loke WM, Proudfoot JM, Stewart S, McKinley AJ, Needs PW, Kroon PA, Hodgson JM & Croft KD (2008). Metabolic transformation has a profound effect on anti-inflammatory activity of flavonoids such as quercetin: Lack of association between antioxidant and lipoxygenase inhibitory activity. Biochem Pharmacol 75, 1045-1053. Loke WM, Proudfoot JM, Stewart S, McKinley AJ, Needs PW, Kroon PA, Hodgson JM & Croft KD (2008). Quercetin and its in vivo metabolites inhibit neutrophil-mediated low-density lipoprotein oxidation. J Agric Food Chem 56, 3609-3615. Hollands W, Brett GM, Radreau P, Saha S, Teucher B, Bennett, RN & Kroon PA (2008). Processing blackcurrants dramatically reduces the content and does not enhance the urinary yield of anthocyanins in human subjects. Food Chem 108, 869-878. Suri S, Taylor MA, Verity A, Tribolo S, Kroon PA, Hughes DA, Wilson VG (2008). A comparative study of the effects of quercetin and its glucuronide and sulphate metabolites on human neutrophil function in vitro. Biochem Pharmacol 76, 645-673. Barrington R, Williamson G, Bennett RN, Davis BD, Brodbelt JS, Kroon PA (2009). Absorption, conjugation and efflux of the flavonoids, kaempferol and galangin, using the intestinal CaCo-2/TC7 cell model. J Functional Foods 1, 74-97. Brett GM, Hollands W, Needs PW, Teucher B, Dainty JR, Bennett RN, Davis BD, Brodbelt JS & Kroon PA (2009). Absorption, metabolism and excretion of flavanones from single portions of orange fruit and juice and effects of anthropometric variables and contraceptive pill use on flavanone excretion. Br J Nutr 101, 664-675. http://journals.cambridge.org/repo_A44rP6ra (Personal author link) Winterbone MS, Tribolo S, Needs PW, Kroon PA & Hughes DA (2009). Physiologically relevant metabolites of quercetin have no effect on adhesion molecule or chemokine expression in human vascular smooth muscle cells. Atherosclerosis 202, 431-438. Kay CD, Kroon PA, Cassidy A (2009). The bioactivity of dietary anthocyanins is likely to be mediated by their degradation products. Mol Nutr Food Res 53 Suppl 1:S92-101. Lodi F, Jiménez R, Moreno L, Kroon PA, Needs PW, Hughes DA, Santos-Buelga C, González-Paramás A, Cogolludo A, Duarte J, Perez-Vizcaino F (2009). Glucuronidated and sulfated metabolites of the flavonoid quercetin prevent endothelial dysfunction but lack direct vasodilator effects in rat aorta. Atherosclerosis 204, 34-39. Radreau P, Rhodes JD, Mithen RF, Kroon PA, Sanderson J (2009) Hypoxia-inducible factor-1 (HIF-1) pathway activation by quercetin in human lens epithelial cells. Exp Eye Res 89, 995-1002. Curtis PJ, Kroon PA, Hollands WJ, Walls R, Jenkins G, Kay CD, Cassidy A (2009). Cardiovascular disease risk biomarkers and liver function are not altered following twelve week ingestion of an elderberry extract rich in anthocyanins. J Nutr 139, 2266-2271. Soler A, Romero MP, Saha S, Furniss CSM, Kroon PA, Motilva MJ (2010). Digestion stability and evaluation of the metabolism and transport of olive oil phenols in human small intestinal epithelial Caco2/TC7 cell line. Food Chem, 119, 703-714. Suri S, Liu XH, Rayment S, Hughes DA, Kroon PA, Needs PW, Taylor MA, Tribolo S, Wilson VG (2010). Quercetin and its major metabolites selectively modulate cyclic GMP-dependent relaxations and associated tolerance in pig isolated coronary artery. Br J Pharmacol 159, 566-575. Bartholomé R, Haenen G, Hollman PCH, Bast A, Dagnelie PC, Roos D, Keijer J, Kroon PA, Needs PW, Arts ICW (2010). Deconjugation kinetics of glucuronidated phase-II flavonoid metabolites by ß-glucuronidase from neutrophils. Drug Metab Pharmacokinet 25, 379-387. Pereira-Caro G, Mateos R, Saha S, Madrona A, Espartero JL, Bravo L, Kroon PA (2010). Trans-epithelial transport and metabolism of new lipopohilic ether derivatives of hydroxytyrosol by enterocyte-like CaCo-2/TC7 cells. J Agric Food Chem 58, 11501-11509. Al-Shalmani S, Suri S, Hughes DA, Kroon PA, Needs PW, Taylor MA, Tribolo S, Wilson VG (2011). Quercetin and its principal metabolites, but not myricetin, oppose LPS-induced hyporesponsiveness of the porcine isolated coronary artery. Br J Pharmacol 162, 1485-1497. Curtis PJ, Sampson M, Potter J, Dhatariya K, Kroon PA, Cassidy A (2012). Chronic ingestion of flavan-3-ols and isoflavones improves insulin sensitivity and lipoprotein status and attenuates estimated 10-year CVD risk in medicated postmenopausal women with type 2 diabetes: a one year double-blind randomized controlled trial. Diabetes Care 35, 226-232. Saha S, Hollands W, Needs PW, Ostertag LM, de Roos B, Duthie GG, Kroon PA (2012). Human O-sulfated metabolites of (-)-epicatechin and methyl-(-)-epicatechin are poor substrates for commercial aryl-sulfatases: Implications for studies concerned with quantifying epicatechin bioavailability. Pharmacol Res 65, 592-602. Lodi F, Tribolo S, Winterbone MS, Needs PW, Hughes DA, Kroon PA (2012). Human quercetin conjugated metabolites attenuate TNF-a-induced changes in vasomodulatory molecules in a HUASMCs/HUVECs co-culture model. Planta Med 78, 1571-1573. Hollands WJ, Hart D, Dainty JR, Hasselwander O, Tiihonen K, Wood R, Kroon PA (2013). Bioavailability of epicatechin and effects on nitric oxide metabolites of an apple flavanol-rich extract supplemented beverage compared to a whole apple puree: a randomized, placebo controlled, crossover trial. Mol Nutr Food Res 57, 1209-1217. Tribolo S, Lodi F, Winterbone MS, Saha S, Needs PW, Suri S, Taylor MA, Wilson VG, Walls R, Cassidy A, Hughes DA, Kroon PA (2013). Human metabolic transformation of quercetin blocks its capacity to decrease eNOS expression and endothelin-1 secretion by human endothelial cells. J Agric Food Chem 61, 8589-8596. Czank C, Cassidy A, Zhang Q, Morrison DJ, Preston T, Kroon PA, Botting NP, Kay CD (2013). Human metabolism and elimination of the anthocyanin, cyanidin-3-glucoside: a 13C-tracer study. Am J Clin Nutr 97, 995-1003. Konic-Ristic A, Srdic-Rajica T, Kardum N, Kroon PA, Hollands WJ, Hayran O, Boyko N, Jorjadze M, Glibetic M (2013). Effects of bioactive-rich extracts of pomegranate, persimmon, nettle, dill, kale and Sideritis and isolated bioactives on arachidonic acid induced markers of platelet activation and platelet-leucocyte aggregation. J Sci Food Agric 93, 3581-3587. De Ferrars RM, Czank C, Zhang Q, Botting NP, Kroon PA, Cassidy A, Kay CD (2014). The pharmacokinetics of anthocyanins and their metabolites in humans. Br J Pharmacol 171, 3268-3282. De Ferrars R, Czank C, Saha S, Needs PW, Zhang Q, Raheem KS, Kroon PA, Kay CD (2014). Methods for isolating, identifying and quantifying anthocyanin metabolites in clinical samples. Anal Chem 86, 10052-10058. Danesi F, Kroon PA, Saha S, de Biase D, D'Antuono LF, Bordoni A (2014). Mixed pro- and anti-oxidative effects of pomegranate polyphenols in cultured cells. Int J Mol Sci 15, 19458-19471. Barrington RD, Needs PW, Williamson G, Kroon PA. MK571 inhibits phase-2 conjugation of flavonols by Caco-2/TC7 cells, but does not specifically inhibit their apical efflux. Biochem Pharmacol 95, 193-200. Cerezo AB, Winterbone MS, Moyle CW, Needs PW, Kroon PA (2015). Molecular structure-function relationship of dietary polyphenols for inhibiting VEGF-induced VEGFR-2 activity. Mol Nutr Food Res. 59, 2119-2131. doi: 10.1002/mnfr.201500407. Gornas P, Redenkovs V, Pugacheva I, Soliven A, Needs PW, Kroon PA (2016). Varied composition of tocochromanols in different types of bran: Rye, wheat, oat, spelt, buckwheat, corn and rice. Int J Food Properties 19, 1757-1764. Dower JI, Geleijnse JM, Kroon PA, Philo M, Mensink M, Kromhout D, Hollman PCH (2016). Does epicatechin contribute to the acute vascular function effects of dark chocolate? A randomised, crossover study. Mol Nutr Food Res 60, 2379-2386. Poór M, Boda G, Needs PW, Kroon PA, Lemli B, Bencsik T (2017). Interaction of quercetin and its metabolites with warfarin: displacement of warfarin from serum albumin and inhibition of CYP2C9 enzyme. Biomed Pharmacol 88, 574-581. Hollands WJ*, Voorspoels S*, Jacobs G, Aaby K, Meisland A, Garcia-Villalbad R, Tomas-Barberan F, Piskula MJ, Mawson D, Vovk I, Needs PW and Kroon PA (2017). Development, validation and evaluation of an analytical method for the determination of monomeric and oligomeric procyanidins in apple extracts. J Chromatogr A 1495, 46-56. Van Rymenant E, Grootaert C, Beerens K, Needs P, Kroon P, Kerimi A, Williamson G, García Villalba R, González-Sarrías A, Tomas-Barberan F, Van Camp J, Van de Voorde J. Vasorelaxant activity of twenty-one physiologically relevant (poly)phenolic metabolites on isolated mouse arteries. Mol Nutr Food Res 13, 4331-4335. Perez-Moral N, Saha S, Philo M, Hart DJ, Winterbone MS, Hollands WJ, Spurr M, Bows J, vander Velpen V, Kroon PA*, Curtis PJC (2018) Comparative bio-accessibility, bioavailability and bioequivalence of quercetin, apigenin, glucoraphanin and carotenoids from freeze-dried vegetables incorporated into a baked snack versus minimally processed vegetables: Evidence from in vitro models and a human bioavailability study. J Func Food 48, 410-419. Poor M, Boda G, Kunsagi-Mate S, Needs PW, Kroon PA, Lemli B (2018). Fluorescence spectroscopic evaluation of the interactions of quercetin, isorhamnetin, and quercetin-3 '-sulfate with different albumins. J Luminescence 194, 156-163. Wu Q, Kroon PA, Shao HJ, Needs PW, Yang XB (2018). Differential effects of quercetin and two of its derivatives, isorhamnetin and isorhamnetin-3-glucuronide, in inhibiting the proliferation of human breast cancer MCF-7 cells. J Agric Food Chem 66, 7181-7189. Wu Q, Needs PW, Lu YL, Kroon PA, Ren DY, Yang XB (2018). Different antitumor effects of quercetin, quercetin-3'-sulfate and quercetin-3-glucuronide in human breast cancer MCF-7 cells. Food Func 9, 1736-1746.
Start Year 2010
 
Description Bespoke synthesis of polyphenol human metabolites 
Organisation University of Nottingham
Country United Kingdom 
Sector Academic/University 
PI Contribution We used synthetic organic chemistry routes to make phase-2 conjugates of various dietary flavonoids and phenolic acids and supplied these to various collaborators for testing of biological activity. These compounds are not available commercially and for many of them they are unique to our lab.
Collaborator Contribution Partners have used these physiologically relevant metabolites to test their biological activity using in vitro models such as cultured mammalian cell models, etc... They have also used them as authentic standards to facilitate identification and quantification of metabolites in human and animal tissues including blood, urine and faeces.
Impact Petri N, Tannergen C, Holst B, Mellon FA, Bao Y, Plumb GW, Bacon J, O'Leary KA, Kroon PA, Knutson L, Forsell P, Eriksson T, Lennernas H & Williamson G (2003) "Absorption/metabolism of sulforaphane and quercetin, and regulation of phase II enzymes, in human jejenum in vivo". Drug Metab Disposition 31, 805-813.Nemeth K, Plumb GW, Berrin J-G, Juge N, Jacob R, Naim HY, Williamson G, Swallow DM, Kroon PA (2003) "Deglycosylation by small intestinal epithelial cell ? glucosidases is a critical step in the absorption and metabolism of dietary flavonoid glycosides in humans. Eur J Nutr 42, 29-42.Kroon PA, Clifford MN, Crozier A, Day AJ, Donovan JL, Manach C & Williamson G (2004). How should we assess the effects of exposure to dietary polyphenols in vitro? Am J Clin Nutr 80, 15-21. Dupont MS, Day AJ, Bennett RN, Mellon FA & Kroon PA (2004) "Absorption of kaempferol-3-glucuronide from endive in humans". Eur J Clin Nutr 58, 947-954. Needs PW, Kroon PA (2006) Convenient syntheses of metabolically important quercetin glucuronides and sulfates. Tetrahedron 62, 6862-6868. Davis BD, Needs PW, Kroon PA & Brodbelt (2006) Identification of isomeric flavonoid glucuronides in urine and plasma by metal complexation and liquid chromatography/tandem mass spectrometry. J Mass Spectrom 41, 911-920. Tribolo S, Lodi F, Connor C, Suri S, Wilson V, Taylor M, Needs PW, Kroon PA, Hughes DA (2008). Comparative effects of quercetin and its predominant human metabolites on adhesion molecule expression in activated human vascular endothelial cells. Atherosclerosis 197, 50-56. Loke WM, Proudfoot JM, Stewart S, McKinley AJ, Needs PW, Kroon PA, Hodgson JM & Croft KD (2008). Metabolic transformation has a profound effect on anti-inflammatory activity of flavonoids such as quercetin: Lack of association between antioxidant and lipoxygenase inhibitory activity. Biochem Pharmacol 75, 1045-1053. Loke WM, Proudfoot JM, Stewart S, McKinley AJ, Needs PW, Kroon PA, Hodgson JM & Croft KD (2008). Quercetin and its in vivo metabolites inhibit neutrophil-mediated low-density lipoprotein oxidation. J Agric Food Chem 56, 3609-3615. Hollands W, Brett GM, Radreau P, Saha S, Teucher B, Bennett, RN & Kroon PA (2008). Processing blackcurrants dramatically reduces the content and does not enhance the urinary yield of anthocyanins in human subjects. Food Chem 108, 869-878. Suri S, Taylor MA, Verity A, Tribolo S, Kroon PA, Hughes DA, Wilson VG (2008). A comparative study of the effects of quercetin and its glucuronide and sulphate metabolites on human neutrophil function in vitro. Biochem Pharmacol 76, 645-673. Barrington R, Williamson G, Bennett RN, Davis BD, Brodbelt JS, Kroon PA (2009). Absorption, conjugation and efflux of the flavonoids, kaempferol and galangin, using the intestinal CaCo-2/TC7 cell model. J Functional Foods 1, 74-97. Brett GM, Hollands W, Needs PW, Teucher B, Dainty JR, Bennett RN, Davis BD, Brodbelt JS & Kroon PA (2009). Absorption, metabolism and excretion of flavanones from single portions of orange fruit and juice and effects of anthropometric variables and contraceptive pill use on flavanone excretion. Br J Nutr 101, 664-675. http://journals.cambridge.org/repo_A44rP6ra (Personal author link) Winterbone MS, Tribolo S, Needs PW, Kroon PA & Hughes DA (2009). Physiologically relevant metabolites of quercetin have no effect on adhesion molecule or chemokine expression in human vascular smooth muscle cells. Atherosclerosis 202, 431-438. Kay CD, Kroon PA, Cassidy A (2009). The bioactivity of dietary anthocyanins is likely to be mediated by their degradation products. Mol Nutr Food Res 53 Suppl 1:S92-101. Lodi F, Jiménez R, Moreno L, Kroon PA, Needs PW, Hughes DA, Santos-Buelga C, González-Paramás A, Cogolludo A, Duarte J, Perez-Vizcaino F (2009). Glucuronidated and sulfated metabolites of the flavonoid quercetin prevent endothelial dysfunction but lack direct vasodilator effects in rat aorta. Atherosclerosis 204, 34-39. Radreau P, Rhodes JD, Mithen RF, Kroon PA, Sanderson J (2009) Hypoxia-inducible factor-1 (HIF-1) pathway activation by quercetin in human lens epithelial cells. Exp Eye Res 89, 995-1002. Curtis PJ, Kroon PA, Hollands WJ, Walls R, Jenkins G, Kay CD, Cassidy A (2009). Cardiovascular disease risk biomarkers and liver function are not altered following twelve week ingestion of an elderberry extract rich in anthocyanins. J Nutr 139, 2266-2271. Soler A, Romero MP, Saha S, Furniss CSM, Kroon PA, Motilva MJ (2010). Digestion stability and evaluation of the metabolism and transport of olive oil phenols in human small intestinal epithelial Caco2/TC7 cell line. Food Chem, 119, 703-714. Suri S, Liu XH, Rayment S, Hughes DA, Kroon PA, Needs PW, Taylor MA, Tribolo S, Wilson VG (2010). Quercetin and its major metabolites selectively modulate cyclic GMP-dependent relaxations and associated tolerance in pig isolated coronary artery. Br J Pharmacol 159, 566-575. Bartholomé R, Haenen G, Hollman PCH, Bast A, Dagnelie PC, Roos D, Keijer J, Kroon PA, Needs PW, Arts ICW (2010). Deconjugation kinetics of glucuronidated phase-II flavonoid metabolites by ß-glucuronidase from neutrophils. Drug Metab Pharmacokinet 25, 379-387. Pereira-Caro G, Mateos R, Saha S, Madrona A, Espartero JL, Bravo L, Kroon PA (2010). Trans-epithelial transport and metabolism of new lipopohilic ether derivatives of hydroxytyrosol by enterocyte-like CaCo-2/TC7 cells. J Agric Food Chem 58, 11501-11509. Al-Shalmani S, Suri S, Hughes DA, Kroon PA, Needs PW, Taylor MA, Tribolo S, Wilson VG (2011). Quercetin and its principal metabolites, but not myricetin, oppose LPS-induced hyporesponsiveness of the porcine isolated coronary artery. Br J Pharmacol 162, 1485-1497. Curtis PJ, Sampson M, Potter J, Dhatariya K, Kroon PA, Cassidy A (2012). Chronic ingestion of flavan-3-ols and isoflavones improves insulin sensitivity and lipoprotein status and attenuates estimated 10-year CVD risk in medicated postmenopausal women with type 2 diabetes: a one year double-blind randomized controlled trial. Diabetes Care 35, 226-232. Saha S, Hollands W, Needs PW, Ostertag LM, de Roos B, Duthie GG, Kroon PA (2012). Human O-sulfated metabolites of (-)-epicatechin and methyl-(-)-epicatechin are poor substrates for commercial aryl-sulfatases: Implications for studies concerned with quantifying epicatechin bioavailability. Pharmacol Res 65, 592-602. Lodi F, Tribolo S, Winterbone MS, Needs PW, Hughes DA, Kroon PA (2012). Human quercetin conjugated metabolites attenuate TNF-a-induced changes in vasomodulatory molecules in a HUASMCs/HUVECs co-culture model. Planta Med 78, 1571-1573. Hollands WJ, Hart D, Dainty JR, Hasselwander O, Tiihonen K, Wood R, Kroon PA (2013). Bioavailability of epicatechin and effects on nitric oxide metabolites of an apple flavanol-rich extract supplemented beverage compared to a whole apple puree: a randomized, placebo controlled, crossover trial. Mol Nutr Food Res 57, 1209-1217. Tribolo S, Lodi F, Winterbone MS, Saha S, Needs PW, Suri S, Taylor MA, Wilson VG, Walls R, Cassidy A, Hughes DA, Kroon PA (2013). Human metabolic transformation of quercetin blocks its capacity to decrease eNOS expression and endothelin-1 secretion by human endothelial cells. J Agric Food Chem 61, 8589-8596. Czank C, Cassidy A, Zhang Q, Morrison DJ, Preston T, Kroon PA, Botting NP, Kay CD (2013). Human metabolism and elimination of the anthocyanin, cyanidin-3-glucoside: a 13C-tracer study. Am J Clin Nutr 97, 995-1003. Konic-Ristic A, Srdic-Rajica T, Kardum N, Kroon PA, Hollands WJ, Hayran O, Boyko N, Jorjadze M, Glibetic M (2013). Effects of bioactive-rich extracts of pomegranate, persimmon, nettle, dill, kale and Sideritis and isolated bioactives on arachidonic acid induced markers of platelet activation and platelet-leucocyte aggregation. J Sci Food Agric 93, 3581-3587. De Ferrars RM, Czank C, Zhang Q, Botting NP, Kroon PA, Cassidy A, Kay CD (2014). The pharmacokinetics of anthocyanins and their metabolites in humans. Br J Pharmacol 171, 3268-3282. De Ferrars R, Czank C, Saha S, Needs PW, Zhang Q, Raheem KS, Kroon PA, Kay CD (2014). Methods for isolating, identifying and quantifying anthocyanin metabolites in clinical samples. Anal Chem 86, 10052-10058. Danesi F, Kroon PA, Saha S, de Biase D, D'Antuono LF, Bordoni A (2014). Mixed pro- and anti-oxidative effects of pomegranate polyphenols in cultured cells. Int J Mol Sci 15, 19458-19471. Barrington RD, Needs PW, Williamson G, Kroon PA. MK571 inhibits phase-2 conjugation of flavonols by Caco-2/TC7 cells, but does not specifically inhibit their apical efflux. Biochem Pharmacol 95, 193-200. Cerezo AB, Winterbone MS, Moyle CW, Needs PW, Kroon PA (2015). Molecular structure-function relationship of dietary polyphenols for inhibiting VEGF-induced VEGFR-2 activity. Mol Nutr Food Res. 59, 2119-2131. doi: 10.1002/mnfr.201500407. Gornas P, Redenkovs V, Pugacheva I, Soliven A, Needs PW, Kroon PA (2016). Varied composition of tocochromanols in different types of bran: Rye, wheat, oat, spelt, buckwheat, corn and rice. Int J Food Properties 19, 1757-1764. Dower JI, Geleijnse JM, Kroon PA, Philo M, Mensink M, Kromhout D, Hollman PCH (2016). Does epicatechin contribute to the acute vascular function effects of dark chocolate? A randomised, crossover study. Mol Nutr Food Res 60, 2379-2386. Poór M, Boda G, Needs PW, Kroon PA, Lemli B, Bencsik T (2017). Interaction of quercetin and its metabolites with warfarin: displacement of warfarin from serum albumin and inhibition of CYP2C9 enzyme. Biomed Pharmacol 88, 574-581. Hollands WJ*, Voorspoels S*, Jacobs G, Aaby K, Meisland A, Garcia-Villalbad R, Tomas-Barberan F, Piskula MJ, Mawson D, Vovk I, Needs PW and Kroon PA (2017). Development, validation and evaluation of an analytical method for the determination of monomeric and oligomeric procyanidins in apple extracts. J Chromatogr A 1495, 46-56. Van Rymenant E, Grootaert C, Beerens K, Needs P, Kroon P, Kerimi A, Williamson G, García Villalba R, González-Sarrías A, Tomas-Barberan F, Van Camp J, Van de Voorde J. Vasorelaxant activity of twenty-one physiologically relevant (poly)phenolic metabolites on isolated mouse arteries. Mol Nutr Food Res 13, 4331-4335. Perez-Moral N, Saha S, Philo M, Hart DJ, Winterbone MS, Hollands WJ, Spurr M, Bows J, vander Velpen V, Kroon PA*, Curtis PJC (2018) Comparative bio-accessibility, bioavailability and bioequivalence of quercetin, apigenin, glucoraphanin and carotenoids from freeze-dried vegetables incorporated into a baked snack versus minimally processed vegetables: Evidence from in vitro models and a human bioavailability study. J Func Food 48, 410-419. Poor M, Boda G, Kunsagi-Mate S, Needs PW, Kroon PA, Lemli B (2018). Fluorescence spectroscopic evaluation of the interactions of quercetin, isorhamnetin, and quercetin-3 '-sulfate with different albumins. J Luminescence 194, 156-163. Wu Q, Kroon PA, Shao HJ, Needs PW, Yang XB (2018). Differential effects of quercetin and two of its derivatives, isorhamnetin and isorhamnetin-3-glucuronide, in inhibiting the proliferation of human breast cancer MCF-7 cells. J Agric Food Chem 66, 7181-7189. Wu Q, Needs PW, Lu YL, Kroon PA, Ren DY, Yang XB (2018). Different antitumor effects of quercetin, quercetin-3'-sulfate and quercetin-3-glucuronide in human breast cancer MCF-7 cells. Food Func 9, 1736-1746.
Start Year 2010
 
Description Bespoke synthesis of polyphenol human metabolites 
Organisation University of Perth
Country Australia 
Sector Academic/University 
PI Contribution We used synthetic organic chemistry routes to make phase-2 conjugates of various dietary flavonoids and phenolic acids and supplied these to various collaborators for testing of biological activity. These compounds are not available commercially and for many of them they are unique to our lab.
Collaborator Contribution Partners have used these physiologically relevant metabolites to test their biological activity using in vitro models such as cultured mammalian cell models, etc... They have also used them as authentic standards to facilitate identification and quantification of metabolites in human and animal tissues including blood, urine and faeces.
Impact Petri N, Tannergen C, Holst B, Mellon FA, Bao Y, Plumb GW, Bacon J, O'Leary KA, Kroon PA, Knutson L, Forsell P, Eriksson T, Lennernas H & Williamson G (2003) "Absorption/metabolism of sulforaphane and quercetin, and regulation of phase II enzymes, in human jejenum in vivo". Drug Metab Disposition 31, 805-813.Nemeth K, Plumb GW, Berrin J-G, Juge N, Jacob R, Naim HY, Williamson G, Swallow DM, Kroon PA (2003) "Deglycosylation by small intestinal epithelial cell ? glucosidases is a critical step in the absorption and metabolism of dietary flavonoid glycosides in humans. Eur J Nutr 42, 29-42.Kroon PA, Clifford MN, Crozier A, Day AJ, Donovan JL, Manach C & Williamson G (2004). How should we assess the effects of exposure to dietary polyphenols in vitro? Am J Clin Nutr 80, 15-21. Dupont MS, Day AJ, Bennett RN, Mellon FA & Kroon PA (2004) "Absorption of kaempferol-3-glucuronide from endive in humans". Eur J Clin Nutr 58, 947-954. Needs PW, Kroon PA (2006) Convenient syntheses of metabolically important quercetin glucuronides and sulfates. Tetrahedron 62, 6862-6868. Davis BD, Needs PW, Kroon PA & Brodbelt (2006) Identification of isomeric flavonoid glucuronides in urine and plasma by metal complexation and liquid chromatography/tandem mass spectrometry. J Mass Spectrom 41, 911-920. Tribolo S, Lodi F, Connor C, Suri S, Wilson V, Taylor M, Needs PW, Kroon PA, Hughes DA (2008). Comparative effects of quercetin and its predominant human metabolites on adhesion molecule expression in activated human vascular endothelial cells. Atherosclerosis 197, 50-56. Loke WM, Proudfoot JM, Stewart S, McKinley AJ, Needs PW, Kroon PA, Hodgson JM & Croft KD (2008). Metabolic transformation has a profound effect on anti-inflammatory activity of flavonoids such as quercetin: Lack of association between antioxidant and lipoxygenase inhibitory activity. Biochem Pharmacol 75, 1045-1053. Loke WM, Proudfoot JM, Stewart S, McKinley AJ, Needs PW, Kroon PA, Hodgson JM & Croft KD (2008). Quercetin and its in vivo metabolites inhibit neutrophil-mediated low-density lipoprotein oxidation. J Agric Food Chem 56, 3609-3615. Hollands W, Brett GM, Radreau P, Saha S, Teucher B, Bennett, RN & Kroon PA (2008). Processing blackcurrants dramatically reduces the content and does not enhance the urinary yield of anthocyanins in human subjects. Food Chem 108, 869-878. Suri S, Taylor MA, Verity A, Tribolo S, Kroon PA, Hughes DA, Wilson VG (2008). A comparative study of the effects of quercetin and its glucuronide and sulphate metabolites on human neutrophil function in vitro. Biochem Pharmacol 76, 645-673. Barrington R, Williamson G, Bennett RN, Davis BD, Brodbelt JS, Kroon PA (2009). Absorption, conjugation and efflux of the flavonoids, kaempferol and galangin, using the intestinal CaCo-2/TC7 cell model. J Functional Foods 1, 74-97. Brett GM, Hollands W, Needs PW, Teucher B, Dainty JR, Bennett RN, Davis BD, Brodbelt JS & Kroon PA (2009). Absorption, metabolism and excretion of flavanones from single portions of orange fruit and juice and effects of anthropometric variables and contraceptive pill use on flavanone excretion. Br J Nutr 101, 664-675. http://journals.cambridge.org/repo_A44rP6ra (Personal author link) Winterbone MS, Tribolo S, Needs PW, Kroon PA & Hughes DA (2009). Physiologically relevant metabolites of quercetin have no effect on adhesion molecule or chemokine expression in human vascular smooth muscle cells. Atherosclerosis 202, 431-438. Kay CD, Kroon PA, Cassidy A (2009). The bioactivity of dietary anthocyanins is likely to be mediated by their degradation products. Mol Nutr Food Res 53 Suppl 1:S92-101. Lodi F, Jiménez R, Moreno L, Kroon PA, Needs PW, Hughes DA, Santos-Buelga C, González-Paramás A, Cogolludo A, Duarte J, Perez-Vizcaino F (2009). Glucuronidated and sulfated metabolites of the flavonoid quercetin prevent endothelial dysfunction but lack direct vasodilator effects in rat aorta. Atherosclerosis 204, 34-39. Radreau P, Rhodes JD, Mithen RF, Kroon PA, Sanderson J (2009) Hypoxia-inducible factor-1 (HIF-1) pathway activation by quercetin in human lens epithelial cells. Exp Eye Res 89, 995-1002. Curtis PJ, Kroon PA, Hollands WJ, Walls R, Jenkins G, Kay CD, Cassidy A (2009). Cardiovascular disease risk biomarkers and liver function are not altered following twelve week ingestion of an elderberry extract rich in anthocyanins. J Nutr 139, 2266-2271. Soler A, Romero MP, Saha S, Furniss CSM, Kroon PA, Motilva MJ (2010). Digestion stability and evaluation of the metabolism and transport of olive oil phenols in human small intestinal epithelial Caco2/TC7 cell line. Food Chem, 119, 703-714. Suri S, Liu XH, Rayment S, Hughes DA, Kroon PA, Needs PW, Taylor MA, Tribolo S, Wilson VG (2010). Quercetin and its major metabolites selectively modulate cyclic GMP-dependent relaxations and associated tolerance in pig isolated coronary artery. Br J Pharmacol 159, 566-575. Bartholomé R, Haenen G, Hollman PCH, Bast A, Dagnelie PC, Roos D, Keijer J, Kroon PA, Needs PW, Arts ICW (2010). Deconjugation kinetics of glucuronidated phase-II flavonoid metabolites by ß-glucuronidase from neutrophils. Drug Metab Pharmacokinet 25, 379-387. Pereira-Caro G, Mateos R, Saha S, Madrona A, Espartero JL, Bravo L, Kroon PA (2010). Trans-epithelial transport and metabolism of new lipopohilic ether derivatives of hydroxytyrosol by enterocyte-like CaCo-2/TC7 cells. J Agric Food Chem 58, 11501-11509. Al-Shalmani S, Suri S, Hughes DA, Kroon PA, Needs PW, Taylor MA, Tribolo S, Wilson VG (2011). Quercetin and its principal metabolites, but not myricetin, oppose LPS-induced hyporesponsiveness of the porcine isolated coronary artery. Br J Pharmacol 162, 1485-1497. Curtis PJ, Sampson M, Potter J, Dhatariya K, Kroon PA, Cassidy A (2012). Chronic ingestion of flavan-3-ols and isoflavones improves insulin sensitivity and lipoprotein status and attenuates estimated 10-year CVD risk in medicated postmenopausal women with type 2 diabetes: a one year double-blind randomized controlled trial. Diabetes Care 35, 226-232. Saha S, Hollands W, Needs PW, Ostertag LM, de Roos B, Duthie GG, Kroon PA (2012). Human O-sulfated metabolites of (-)-epicatechin and methyl-(-)-epicatechin are poor substrates for commercial aryl-sulfatases: Implications for studies concerned with quantifying epicatechin bioavailability. Pharmacol Res 65, 592-602. Lodi F, Tribolo S, Winterbone MS, Needs PW, Hughes DA, Kroon PA (2012). Human quercetin conjugated metabolites attenuate TNF-a-induced changes in vasomodulatory molecules in a HUASMCs/HUVECs co-culture model. Planta Med 78, 1571-1573. Hollands WJ, Hart D, Dainty JR, Hasselwander O, Tiihonen K, Wood R, Kroon PA (2013). Bioavailability of epicatechin and effects on nitric oxide metabolites of an apple flavanol-rich extract supplemented beverage compared to a whole apple puree: a randomized, placebo controlled, crossover trial. Mol Nutr Food Res 57, 1209-1217. Tribolo S, Lodi F, Winterbone MS, Saha S, Needs PW, Suri S, Taylor MA, Wilson VG, Walls R, Cassidy A, Hughes DA, Kroon PA (2013). Human metabolic transformation of quercetin blocks its capacity to decrease eNOS expression and endothelin-1 secretion by human endothelial cells. J Agric Food Chem 61, 8589-8596. Czank C, Cassidy A, Zhang Q, Morrison DJ, Preston T, Kroon PA, Botting NP, Kay CD (2013). Human metabolism and elimination of the anthocyanin, cyanidin-3-glucoside: a 13C-tracer study. Am J Clin Nutr 97, 995-1003. Konic-Ristic A, Srdic-Rajica T, Kardum N, Kroon PA, Hollands WJ, Hayran O, Boyko N, Jorjadze M, Glibetic M (2013). Effects of bioactive-rich extracts of pomegranate, persimmon, nettle, dill, kale and Sideritis and isolated bioactives on arachidonic acid induced markers of platelet activation and platelet-leucocyte aggregation. J Sci Food Agric 93, 3581-3587. De Ferrars RM, Czank C, Zhang Q, Botting NP, Kroon PA, Cassidy A, Kay CD (2014). The pharmacokinetics of anthocyanins and their metabolites in humans. Br J Pharmacol 171, 3268-3282. De Ferrars R, Czank C, Saha S, Needs PW, Zhang Q, Raheem KS, Kroon PA, Kay CD (2014). Methods for isolating, identifying and quantifying anthocyanin metabolites in clinical samples. Anal Chem 86, 10052-10058. Danesi F, Kroon PA, Saha S, de Biase D, D'Antuono LF, Bordoni A (2014). Mixed pro- and anti-oxidative effects of pomegranate polyphenols in cultured cells. Int J Mol Sci 15, 19458-19471. Barrington RD, Needs PW, Williamson G, Kroon PA. MK571 inhibits phase-2 conjugation of flavonols by Caco-2/TC7 cells, but does not specifically inhibit their apical efflux. Biochem Pharmacol 95, 193-200. Cerezo AB, Winterbone MS, Moyle CW, Needs PW, Kroon PA (2015). Molecular structure-function relationship of dietary polyphenols for inhibiting VEGF-induced VEGFR-2 activity. Mol Nutr Food Res. 59, 2119-2131. doi: 10.1002/mnfr.201500407. Gornas P, Redenkovs V, Pugacheva I, Soliven A, Needs PW, Kroon PA (2016). Varied composition of tocochromanols in different types of bran: Rye, wheat, oat, spelt, buckwheat, corn and rice. Int J Food Properties 19, 1757-1764. Dower JI, Geleijnse JM, Kroon PA, Philo M, Mensink M, Kromhout D, Hollman PCH (2016). Does epicatechin contribute to the acute vascular function effects of dark chocolate? A randomised, crossover study. Mol Nutr Food Res 60, 2379-2386. Poór M, Boda G, Needs PW, Kroon PA, Lemli B, Bencsik T (2017). Interaction of quercetin and its metabolites with warfarin: displacement of warfarin from serum albumin and inhibition of CYP2C9 enzyme. Biomed Pharmacol 88, 574-581. Hollands WJ*, Voorspoels S*, Jacobs G, Aaby K, Meisland A, Garcia-Villalbad R, Tomas-Barberan F, Piskula MJ, Mawson D, Vovk I, Needs PW and Kroon PA (2017). Development, validation and evaluation of an analytical method for the determination of monomeric and oligomeric procyanidins in apple extracts. J Chromatogr A 1495, 46-56. Van Rymenant E, Grootaert C, Beerens K, Needs P, Kroon P, Kerimi A, Williamson G, García Villalba R, González-Sarrías A, Tomas-Barberan F, Van Camp J, Van de Voorde J. Vasorelaxant activity of twenty-one physiologically relevant (poly)phenolic metabolites on isolated mouse arteries. Mol Nutr Food Res 13, 4331-4335. Perez-Moral N, Saha S, Philo M, Hart DJ, Winterbone MS, Hollands WJ, Spurr M, Bows J, vander Velpen V, Kroon PA*, Curtis PJC (2018) Comparative bio-accessibility, bioavailability and bioequivalence of quercetin, apigenin, glucoraphanin and carotenoids from freeze-dried vegetables incorporated into a baked snack versus minimally processed vegetables: Evidence from in vitro models and a human bioavailability study. J Func Food 48, 410-419. Poor M, Boda G, Kunsagi-Mate S, Needs PW, Kroon PA, Lemli B (2018). Fluorescence spectroscopic evaluation of the interactions of quercetin, isorhamnetin, and quercetin-3 '-sulfate with different albumins. J Luminescence 194, 156-163. Wu Q, Kroon PA, Shao HJ, Needs PW, Yang XB (2018). Differential effects of quercetin and two of its derivatives, isorhamnetin and isorhamnetin-3-glucuronide, in inhibiting the proliferation of human breast cancer MCF-7 cells. J Agric Food Chem 66, 7181-7189. Wu Q, Needs PW, Lu YL, Kroon PA, Ren DY, Yang XB (2018). Different antitumor effects of quercetin, quercetin-3'-sulfate and quercetin-3-glucuronide in human breast cancer MCF-7 cells. Food Func 9, 1736-1746.
Start Year 2010
 
Description CE collaboration with Biopolymer Research and Engineering UANDES 
Organisation University of the Andes
Country Colombia 
Sector Academic/University 
PI Contribution Intellectual input on starch digestion of food, prediction of glycaemic responses and training in laboratory techniques.
Collaborator Contribution Novel materials and expertise on biopolymer/food characterization
Impact Too early to report on outputs or outcomes
Start Year 2019
 
Description Collaboration to assess the prebiotic activity of orange polyphenols and fibre 
Organisation Universidade de São Paulo
Country Brazil 
Sector Academic/University 
PI Contribution We provided expertise in the analysis of polyphenol human metabolites in complex human tissue samples and the access to LC-MS platforms and extraction and analysis protocols for analysing orange flavanones and their human and gut microbiota metabolites.
Collaborator Contribution The USP group provided human samples from a RCT in which participants were fed orange containing flavanones.
Impact 6-month placement of a PGR student from USP at QIB
Start Year 2019
 
Description Collaboration with Protexin for development of functional foods 
Organisation Protexin
Country United Kingdom 
Sector Private 
PI Contribution Collaboration with Protexin for development of functional foods
Collaborator Contribution Collaboration with Protexin for development of functional foods
Impact Collaboration with Protexin for development of functional foods
Start Year 2019
 
Description NCFM with QIB 
Organisation University of Lincoln
Department National Centre for Food Manufacturing (NCFM)
Country United Kingdom 
Sector Academic/University 
PI Contribution Expertise on food structure, digestion and nutrition.
Collaborator Contribution Expertise and facilities for food processing, technical know-how, and links with food technology SMEs
Impact Joint funding proposals and research directives and a knowledge exchange meeting
Start Year 2019
 
Description Norwich Institute for Sustainable Development 
Organisation Earlham Institute
Country United Kingdom 
Sector Academic/University 
PI Contribution Research expertise in food and nutrition
Collaborator Contribution Knowledge and research discipline expertise, experience in policy advisory work, cross-disciplinary communication and outreach and impact
Impact Youtube video Website
Start Year 2021
 
Description Norwich Institute for Sustainable Development 
Organisation John Innes Centre
Country United Kingdom 
Sector Academic/University 
PI Contribution Research expertise in food and nutrition
Collaborator Contribution Knowledge and research discipline expertise, experience in policy advisory work, cross-disciplinary communication and outreach and impact
Impact Youtube video Website
Start Year 2021
 
Description Norwich Institute for Sustainable Development 
Organisation Norwich Research Park
Country United Kingdom 
Sector Private 
PI Contribution Research expertise in food and nutrition
Collaborator Contribution Knowledge and research discipline expertise, experience in policy advisory work, cross-disciplinary communication and outreach and impact
Impact Youtube video Website
Start Year 2021
 
Description Norwich Institute for Sustainable Development 
Organisation Quadram Institute Bioscience
Country United Kingdom 
Sector Academic/University 
PI Contribution Research expertise in food and nutrition
Collaborator Contribution Knowledge and research discipline expertise, experience in policy advisory work, cross-disciplinary communication and outreach and impact
Impact Youtube video Website
Start Year 2021
 
Description Norwich Institute for Sustainable Development 
Organisation The Sainsbury Laboratory
Country United Kingdom 
Sector Academic/University 
PI Contribution Research expertise in food and nutrition
Collaborator Contribution Knowledge and research discipline expertise, experience in policy advisory work, cross-disciplinary communication and outreach and impact
Impact Youtube video Website
Start Year 2021
 
Description Norwich Institute for Sustainable Development 
Organisation University of East Anglia
Department Climate Research Unit
Country United Kingdom 
Sector Academic/University 
PI Contribution Research expertise in food and nutrition
Collaborator Contribution Knowledge and research discipline expertise, experience in policy advisory work, cross-disciplinary communication and outreach and impact
Impact Youtube video Website
Start Year 2021
 
Description Norwich Institute for Sustainable Development 
Organisation University of East Anglia
Department Tyndall Centre
Country United Kingdom 
Sector Academic/University 
PI Contribution Research expertise in food and nutrition
Collaborator Contribution Knowledge and research discipline expertise, experience in policy advisory work, cross-disciplinary communication and outreach and impact
Impact Youtube video Website
Start Year 2021
 
Description Norwich Institute of Healthy Ageing 
Organisation Earlham Institute
Country United Kingdom 
Sector Academic/University 
PI Contribution Contributing with research expertise on food, gut and health which is relevant to the central mission of the institute.
Collaborator Contribution Participated in launch and kick off events and presented overview of QIB research and facilities,
Impact Two events have been held, website launched and open-lines of communication established. The institute launched recently, so it is too early to report outputs and ouctomes. This is a multi-disciplinary collaboration spanning bioscience, social science, public health and integrating regional authorities, industry and health care.
Start Year 2020
 
Description Norwich Institute of Healthy Ageing 
Organisation John Innes Centre
Country United Kingdom 
Sector Academic/University 
PI Contribution Contributing with research expertise on food, gut and health which is relevant to the central mission of the institute.
Collaborator Contribution Participated in launch and kick off events and presented overview of QIB research and facilities,
Impact Two events have been held, website launched and open-lines of communication established. The institute launched recently, so it is too early to report outputs and ouctomes. This is a multi-disciplinary collaboration spanning bioscience, social science, public health and integrating regional authorities, industry and health care.
Start Year 2020
 
Description Norwich Institute of Healthy Ageing 
Organisation NHS England
Country United Kingdom 
Sector Public 
PI Contribution Contributing with research expertise on food, gut and health which is relevant to the central mission of the institute.
Collaborator Contribution Participated in launch and kick off events and presented overview of QIB research and facilities,
Impact Two events have been held, website launched and open-lines of communication established. The institute launched recently, so it is too early to report outputs and ouctomes. This is a multi-disciplinary collaboration spanning bioscience, social science, public health and integrating regional authorities, industry and health care.
Start Year 2020
 
Description Norwich Institute of Healthy Ageing 
Organisation Norfolk County Council
Country United Kingdom 
Sector Public 
PI Contribution Contributing with research expertise on food, gut and health which is relevant to the central mission of the institute.
Collaborator Contribution Participated in launch and kick off events and presented overview of QIB research and facilities,
Impact Two events have been held, website launched and open-lines of communication established. The institute launched recently, so it is too early to report outputs and ouctomes. This is a multi-disciplinary collaboration spanning bioscience, social science, public health and integrating regional authorities, industry and health care.
Start Year 2020
 
Description Norwich Institute of Healthy Ageing 
Organisation Norwich City Council
Country United Kingdom 
Sector Public 
PI Contribution Contributing with research expertise on food, gut and health which is relevant to the central mission of the institute.
Collaborator Contribution Participated in launch and kick off events and presented overview of QIB research and facilities,
Impact Two events have been held, website launched and open-lines of communication established. The institute launched recently, so it is too early to report outputs and ouctomes. This is a multi-disciplinary collaboration spanning bioscience, social science, public health and integrating regional authorities, industry and health care.
Start Year 2020
 
Description Norwich Institute of Healthy Ageing 
Organisation Quadram Institute Bioscience
Country United Kingdom 
Sector Academic/University 
PI Contribution Contributing with research expertise on food, gut and health which is relevant to the central mission of the institute.
Collaborator Contribution Participated in launch and kick off events and presented overview of QIB research and facilities,
Impact Two events have been held, website launched and open-lines of communication established. The institute launched recently, so it is too early to report outputs and ouctomes. This is a multi-disciplinary collaboration spanning bioscience, social science, public health and integrating regional authorities, industry and health care.
Start Year 2020
 
Description Norwich Institute of Healthy Ageing 
Organisation University of East Anglia
Department Health & Social Care Partners
Country United Kingdom 
Sector Charity/Non Profit 
PI Contribution Contributing with research expertise on food, gut and health which is relevant to the central mission of the institute.
Collaborator Contribution Participated in launch and kick off events and presented overview of QIB research and facilities,
Impact Two events have been held, website launched and open-lines of communication established. The institute launched recently, so it is too early to report outputs and ouctomes. This is a multi-disciplinary collaboration spanning bioscience, social science, public health and integrating regional authorities, industry and health care.
Start Year 2020
 
Description Norwich Institute of Healthy Ageing 
Organisation University of East Anglia
Country United Kingdom 
Sector Academic/University 
PI Contribution Contributing with research expertise on food, gut and health which is relevant to the central mission of the institute.
Collaborator Contribution Participated in launch and kick off events and presented overview of QIB research and facilities,
Impact Two events have been held, website launched and open-lines of communication established. The institute launched recently, so it is too early to report outputs and ouctomes. This is a multi-disciplinary collaboration spanning bioscience, social science, public health and integrating regional authorities, industry and health care.
Start Year 2020
 
Description Prebiotic ingredients from orange by-products 
Organisation Universidade de São Paulo
Country Brazil 
Sector Academic/University 
PI Contribution My research team provide expertise in investigating the effects of food components on the gut microbiota structure and metabolic capacity and also the colon models in which to do these experiments and the analytical chemistry methods for quantifying the concentrations of polyphenols and their metabolites and nutrients such as choline and their metabolites
Collaborator Contribution The partner has generated some fractions from orange that may have prebiotic activity and also food products such as cookies that contain these putative prebiotic components that can be tested at QIB.
Impact 9-month placement of USP PGR student at QIB
Start Year 2019
 
Description Quorn 
Organisation Marlow Foods
Country United Kingdom 
Sector Private 
PI Contribution The research was discussed in the context of the business of Marlow Foods (Quorn) to understand the mechanisms underpinning the digestion and bioavailability of proteins, fibre and other nutrients from Quorn products. After negotiations, Marlow Foods agreed to fund a PhD studentship to study these aspects.
Collaborator Contribution Marlow Foods brought the problem in terms of a lack of a mechanistic understanding of the health benefits of Quorn. They contributed financially to the project by fully funding a PhD student (plus an additional student with Imperial College London).
Impact The studentship only started in January 2018, so it is too early to say.
Start Year 2017
 
Description Tara Grauwet KU Leuven 
Organisation University of Leuven
Country Belgium 
Sector Academic/University 
PI Contribution Technical expertise on in vitro lipid digestion and confocal microscopy
Collaborator Contribution Funding from fellowship to visit and conduct collaborative research on the role pf pectin on lipid digestion.
Impact Visits have been postponed due to the Covid-19 pandemic. Currently discussion activities and timelines for future visit.
Start Year 2020
 
Description Teagasc 
Organisation Teagasc
Department Teagasc Food Research Centre
Country Ireland 
Sector Academic/University 
PI Contribution Joint supervision of 2 Walsh Fellowship PhD studentships, funded by Teagasc, and hosted partly at QIB and partly at Teagasc Food research Centres (1). Miss Ana-Isabel Mulet-Cabero supervised by Dr Andre Brodkorb at the Food Research Centre, Moorepark, Fermoy, Ireland (2). Mr Rares-Ionut Birsan, supervised by Dr Dilip Rai at The Ashtown Food Research Centre, Dublin, Ireland
Collaborator Contribution Joint supervision of PhD students, hosted student while placed in Teagasc for 3.5 years in total between the two students
Impact Mulet-Cabero, A. I., A. R. Mackie, P. J. Wilde, M. A. Fenelon and A. Brodkorb (2019). "Structural mechanism and kinetics of in vitro gastric digestion are affected by process-induced changes in bovine milk." Food Hydrocolloids 86: 172-183. Ana-Isabel Mulet-Cabero: PhD student completed successfully, University of East Anglia, December 2018. Thesis title: Effect of Dairy Structures on Gastric Behaviour and Nutrient Digestion Kinetics using a Semi-Dynamic Model
Start Year 2014
 
Description University of Warmia and Mazury 
Organisation University of Warmia and Mazury in Olsztyn
Country Poland 
Sector Academic/University 
PI Contribution Hosted visiting scientist Dr Marek Aljewicz for 3 months to perform experimental study on the interaction of beta glucans and milk proteins
Collaborator Contribution They brought previous experimental results and data to aid the design of the experiments at QIB to support the submission of a manuscript (in preparation), and to contribute to the studies of the visitor's PhD student at UWM
Impact The collaboration is still active. We expect the results will contribute to a joint paper. This is a multidisciplinary collaboration which includes mineral nutrition (bioavailability), microbiology and food physical chemistry.
Start Year 2019
 
Description Zhejiang University of Technology, China 
Organisation Zhejiang University of Technology
Country China 
Sector Academic/University 
PI Contribution We hosted Dr Jianhua Liu as a visiting scientist in my group for one year between August 2016 and August 2017, he received training in colloidal techniques and in vitro digestion methodologies.
Collaborator Contribution Jianhua investigated the functionality of fish oil emulsions during his visit and wrote up two papers, one of which has been recently published
Impact Liu, J. H., W. L. Liu, L. J. Salt, M. J. Ridout, Y. T. Ding and P. J. Wilde (2019). "Fish Oil Emulsions Stabilized with Caseinate Glycated by Dextran: Physicochemical Stability and Gastrointestinal Fate." Journal of Agricultural and Food Chemistry 67(1): 452-462.
Start Year 2016
 
Title Foodstuffs having improved digestion properties 
Description Methods to increase the resistant starch content of peas through combined genomics and processing 
IP Reference GB2014104.0 
Protection Patent application published
Year Protection Granted 2020
Licensed No
Impact The discovery has led to several industrial interactions to further develop the discovery in terms of aiding the development of new food ingredients. This has led to a UKRI super follow on fund application to accelerate exploitation, and specific confidential discussions with individual companies to potentially license the IP towards ingredient development.
 
Title PulseON 
Description A graphical design and trademark for PulseON - a new functional food ingredient. 
IP Reference  
Protection Trade Mark
Year Protection Granted 2019
Licensed No
Impact The trademark is already being used to aid communication with consumers/industry, but it is too early to report on the impact of this.
 
Title SNACK FOOD MANUFACTURE 
Description A method of making a snack food in the form of snack food chips, the method comprising the steps of: a. providing at least one pre-processed vegetable material, the pre-processed vegetable material including at least one bioactive component which comprises a polyphenol or a glucosinolate or a mixture of any two or more thereof, wherein the at least one pre-processed vegetable material is in a freeze-dried form, in an individually quick frozen (IQF) form, in a defrosted, chilled or fresh comminuted form and/or in a defrosted, chilled or fresh pulp form; b. incorporating the at least one pre-processed vegetable material into a dough, wherein the dough is a potato-based dough, and wherein the dough forms individual dough sheet portions, and the snack food is in the form of snack food chips; c. cooking the dough sheet portions to form a cooked snack food in the form of snack food chips, the cooked snack food having a moisture content after the cooking step of from 5 to 25 wt% based on the weight of the cooked snack food, wherein the cooking comprises baking, microwave cooking, infrared cooking or radio frequency (RF) cooking; and d. drying the cooked snack food to form a dried snack food which has a moisture content after the drying step of from 1 to 4 wt%, based on the weight of the dried cooked snack food. 
IP Reference CA3069041 
Protection Patent granted
Year Protection Granted 2019
Licensed Commercial In Confidence
Impact The commercial owner of the patent continues to undertake R&D in this area including via a CASE studentship at QIB and another at PepsiCo Beaumont Park, Leicester, UK.
 
Title SNACK FOOD MANUFACTURE 
Description A method of making a snack food in the form of snack food chips, the method comprising the steps of: a. providing at least one pre-processed vegetable material, the pre-processed vegetable material including at least one bioactive component which comprises a polyphenol or a glucosinolate or a mixture of any two or more thereof, wherein the at least one pre-processed vegetable material is in a freeze-dried form, in an individually quick frozen (IQF) form, in a defrosted, chilled or fresh comminuted form and/or in a defrosted, chilled or fresh pulp form; b. incorporating the at least one pre-processed vegetable material into a dough, wherein the dough is a potato-based dough, and wherein the dough forms individual dough sheet portions, and the snack food is in the form of snack food chips; c. cooking the dough sheet portions to form a cooked snack food in the form of snack food chips, the cooked snack food having a moisture content after the cooking step of from 5 to 25 wt% based on the weight of the cooked snack food, wherein the cooking comprises baking, microwave cooking, infrared cooking or radio frequency (RF) cooking; and d. drying the cooked snack food to form a dried snack food which has a moisture content after the drying step of from 1 to 4 wt%, based on the weight of the dried cooked snack food. 
IP Reference WO2019008088 
Protection Patent granted
Year Protection Granted 2019
Licensed Commercial In Confidence
Impact The commercial owner of the patent continues to undertake R&D in this area including via a CASE studentship at QIB and another at PepsiCo Beaumont Park, Leicester, UK.
 
Title Snack food chips comprising vegetable material 
Description A snack food chip comprises vegetable material including at least one bioactive component which comprises a polyphenol or a glucosinolate or a mixture of any two or more thereof, wherein the at least one bioactive component is present in a concentration of at least 5 mg per 100g of the snack food chip on a dry material basis. The bioactive component may comprise a flavonal, which may be quercetin. The bioactive component may comprise a flavone, which may be apigenin. The bioactive component may comprise a glucosinolate, which may be glucoraphanin. Also disclosed is the use of at least one pre-processed vegetable material in the manufacture of a cooked snack food. 
IP Reference GB2575735 
Protection Patent granted
Year Protection Granted 2020
Licensed Commercial In Confidence
Impact The commercial owner of the patent continues to undertake R&D in this area including via a CASE studentship at QIB and another at PepsiCo Beaumont Park, Leicester, UK.
 
Title Snack food manufacture 
Description A method of making a snack food comprises the steps of: (i) providing at least one pre-processed vegetable material 2 which includes at least one bioactive component comprising a polyphenol or a glucosinolate or a mixture of any two or more thereof, wherein the at least one pre­processed vegetable material is in a freeze-dried form, an individually quick frozen (IQF) form, a defrosted comminuted form and/or a defrosted pulp form; (ii) incorporating the at least one pre-processed vegetable material into a dough 4; and (iii) cooking the dough to form a snack food. Also disclosed is a snack food chip comprising vegetable material including at least one bioactive component which comprises a polyphenol or a glucosinolate or a mixture of any two or more thereof, wherein the at least one bioactive component is present in a concentration of at least 5 mg per 100g of the snack food chip on a dry material basis. Additionally disclosed is the use of at least one pre-processed vegetable material in the manufacture of a cooked snack food. 
IP Reference GB2559436 
Protection Patent granted
Year Protection Granted 2018
Licensed Commercial In Confidence
Impact The commercial owner of the patent continues to undertake R&D in this area including via a CASE studentship at QIB and another at PepsiCo Beaumont Park, Leicester, UK.
 
Title REST study 
Description REST is about white wheat bread with high Resistant Starch, a type of fibre. Wheat bread made from white flour is one of the UK's favourite foods but normally, it has very low levels of fibre. Fibre exerts a protective role in reducing the risk of many common diseases that are diet related. However, in the UK, 91% of the adult population does not meet the recommended fibre intake of 30g per day. A correct fibre intake helps to control blood sugar levels and body weight, reducing the risk of developing type II diabetes and obesity. We have developed a type of wheat white flour that appears similar to conventional white bread flour but has higher resistant starch content (which is a type of fibre). In this study, we want to measure blood sugar after eating a high resistant starch bread and a conventional white bread, low in resistant starch. This will help us understand if high resistant starch bread can help to boost fibre intake of healthy people. In the long term, we might be able to determine if high resistant starch bread can help reduce the risk of common chronic diseases. We are currently recruiting Healthy volunteers, between 18 and 65 years of age, living within 40 miles from the Norwich Research Park 
Type Preventative Intervention - Nutrition and Chemoprevention
Current Stage Of Development Early clinical assessment
Year Development Stage Completed 2020
Development Status Under active development/distribution
Impact n/a 
URL https://quadram.ac.uk/reststudy/
 
Title SAP clinical trial 
Description A food product that delivers high levels of the bioactive compound S-methylcysteine sulfoxide which accumulates in prostate tissue of men with organ confined prostate cancer 
Type Preventative Intervention - Nutrition and Chemoprevention
Current Stage Of Development Early clinical assessment
Year Development Stage Completed 2018
Development Status Actively seeking support
Clinical Trial? Yes
Impact A food product that delivers S-methyl cysteine sulfoxide that accumulates in prostate tissue and may reduce risk of aggressive prostate cancer. A patent has been filed. 
URL https://clinicaltrials.gov/show/NCT02821728
 
Company Name THE SMARTER FOOD COMPANY LIMITED 
Description This is a spin out company from based upon intellectual property from the Food and Health ISP and the Food Innovation and Health ISP. 
Year Established 2018 
Impact The company has recently been established. It has received investment from private equity funds an a grant from Innovate UK. It s currently engaged with developing food products that will enter clinical trials towards end of 2019
 
Description Norwich Science Festival 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Norwich Science Festival took place 18-26 October 2019,with nine days of inspirational exhibitions, sensational shows and an abundance of hands-on science activities for all ages and all levels of knowledge; plus a dedicated learning programme for schools, youth groups and home-educated learners.
Year(s) Of Engagement Activity 2019
URL https://norwichsciencefestival.co.uk/
 
Description 'Heroes of Food Research' Public Engagement activity 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact The purpose of the set of activities was to engage in a dialogue with members of the public in the region where we recruit participants to partake in dietary intervention studies. The activities included two sessions in Norwich's Forum Library public space where visitors could read about the types of studies that were going on, discuss what was involved when volunteering to participate in a study, ask questions of study PIs and study managers, taste intervention foods, complete a short questionnaire, and sign up for future activities which included an evening session with audience participation and voting and a workshop to gather feedback from potential volunteers. As a result of the activities and the significant feedback from the general public and those who had previously participated in human studies, we completely revamped our institute's Human Studies internet pages, recorded feedback and subsequently changed various features of our adverts that invited people to participate in specific studies, and recruited a significant number of new people to our database of interested volunteers.
Year(s) Of Engagement Activity 2015,2016
URL https://quadram.ac.uk/volunteer/
 
Description Association of Clinical Research Professionals International Clinical Trials Day 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact This day of celebration also provides the people who conduct clinical trials with a unique opportunity to raise awareness of clinical trials - and of clinical research as a career option - among the greater public.
Year(s) Of Engagement Activity 2019
URL http://www.clinicaltrialsday.org
 
Description Bioavailability 2018 International Conference, 'Understanding the bioavailability of micronutrients and bioactive compounds for improved public health', the 8th in the series of Bioavailability conferences. 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact Bioavailability 2018 International Conference, 'Understanding the bioavailability of micronutrients and bioactive compounds for improved public health', the 8th in the series of Bioavailability conferences.
Year(s) Of Engagement Activity 2018
 
Description Breeders Day 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Breeders Day is an event organised by the John Innes Centre for to hear first-hand from JIC scientists about the science carried out on site and how it impacts crop breeding and farming. Breeders day takes place at JIC conference centre and at JIC Field Station with talks both indoors and outdoor while walking between plots.
Year(s) Of Engagement Activity 2017,2018
URL https://twitter.com/MarinaCorrad/status/1004666473904734208
 
Description CCG representatives visit to discuss collaborative opportunities for food and health research 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact CCG representatives visit to discuss collaborative opportunities for food and health research
Year(s) Of Engagement Activity 2019
 
Description CCG representatives visit to discuss collaborative opportunities for food and health research 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact CCG representatives visited the Quadram Institute to get to know the clinical trials and research related to cereals and glycaemic response.
Year(s) Of Engagement Activity 2019
 
Description Cereal and Grains Annual meeting 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact The Cereals & Grains meeting brings together a broad audience to discuss advancement in cereals and grains science. We presented a oral presentation during the Starch Technical Session and a poster presentation during the Health Benefits of grain-based macro-and micronutrients.
Year(s) Of Engagement Activity 2018
 
Description Cool Food Facts - primary school BIO outreach event in Lowestoft 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Lowestoft primary school requested the activity for the science day to the School of Biology, UEA (contact - Carl Harrington). The session ran 4 times for Yr2 to Yr6, with approximately 30 students in each session.
Year(s) Of Engagement Activity 2018
 
Description Daily Mail article 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact The Daily Maill published an article in response to a publication in Food Hydrocolloids, which described the use of chickpea PulseON powder to lower glycaemic responses to white bread. When last accessed on 17th feb 2021, the article had 238 shared and 79 comments. The comments provided useful insight into public perception of research. Many readers welcomed healthier white bread, especially for diabetes. Many did not understand the difference between the novel chickpea powder and normal chickpea, besan or gram flour, which are used by many to make bread, but does not offer the same low glycaemic benefits. This insight will be used to adjust future public engagement activities.
Year(s) Of Engagement Activity 2021
URL https://www.dailymail.co.uk/sciencetech/article-9150829/White-bread-rolls-flour-CHICKPEAS-slashes-bl...
 
Description Diabetes primary care regional event 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Patients, carers and/or patient groups
Results and Impact A working group meeting for diabetes care where a presentation on carbohydrates, nutrition and ingredients sparked further discussion about role of primary care providers in providing nutrition advice to patients, and how patients can become more involved in transnational research that aims to deliver healthier carbohydrate food products
Year(s) Of Engagement Activity 2019
 
Description Engagement with government 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Policymakers/politicians
Results and Impact Understanding how research and policy interact and identify gaps in the diabetes research that need to be addressed. Understanding NHS long term and short programme for diabetes prevention and care Discussing funding opportunities for diabetes research
Year(s) Of Engagement Activity 2019
 
Description FDNC & FIH School Activity - Clever Food Trail session with primary school children 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Visited Hethersett Primary School to speak and provide a number of activities on the clever food trail.
Year(s) Of Engagement Activity 2020
 
Description Food Matters Live 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact Purpose was to get an overview of trends in functional food ingredients and consider how novel ingredients being developed as part of transnational research could fit in
Year(s) Of Engagement Activity 2019
 
Description Food Structure & Functionality Symposium 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Engagement via an online mini symposium created to provide information on current developments in food structure design and functionality.
Year(s) Of Engagement Activity 2020
URL https://www.teagasc.ie/news--events/national-events/events/foodstructurefunctionalit.php
 
Description From Seed to Pasta III 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact From Seed to Pasta" is a multidiscplinary conference focused on the remarkable progress in durum wheat science and the increasing concern on the future availability of durum grain due to climate change, a matter with fundamental socio-economic implications in the Mediterranean countries where durum-derived products are a staple.
Oral presentation on "digestibility of processed durum wheat with increased resistant starch"
Year(s) Of Engagement Activity 2018
 
Description Future Food Tech Meeting 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact Future Food-Tech is an international innovation and investment summit held annually in London, San Francisco and New York. Harnessing technology to address the critical issues facing the world's food industry, the summit brings together global food-tech brands, investors and entrepreneurs to accelerate the adoption and commercialisation of new products and solutions. The 2018 programme focused on plant-based and cultured proteins, personalised nutrition, ingredient innovation, gene-editing for health and sustainability, and food waste reduction, featuring 60 game-changing speakers and the most exciting start-ups on the market. Several companies expressed an interest in QIB research and exchanged business cards for follow up activities.
Year(s) Of Engagement Activity 2018
URL https://futurefoodtechlondon.com/
 
Description Innovation for Nutrition Outcomes 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact A learning series to brainstorm innovation for nutrition, with a strong focus on needs of LMICs.
Invited participants included a academics from overseas (UK, EU, USA, LMICs especially in Africa, venture capitalists, industries with disruptive technologies, and representatives of other not-for profit organisations). The event spanned over 3 days and featured roundtable and breakout discussion, presentations and pitches from the attendees. The event resulted in change in own perspectives around priorities for nutrition innovation in LMICs. Other participants also reported a change in perceptions/understanding. For example, following a roundtable discussion of challenges in improving nutrition and a pitch on ideas around changing food structure/processing for improved nutrition, others attendees commented that they were not previously aware that so many fundamental concepts in nutrition (bioaccessibility, bioavailablity) were not understood.
Year(s) Of Engagement Activity 2020
 
Description International Clinical Trial Day 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact International Clinical Trial Day
Year(s) Of Engagement Activity 2019
 
Description International bio-accessibility and food structure training workshop 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Training on laboratory technqiues and theory behind nutrient bioaccessibility and food stcrutrue and its importance in analysis of nutritional value of food. Visitors from LMICs reported that they learned something new and requested that training material could be shared so that they could present this information in lectures to undergraduate and postgraduate students.
Year(s) Of Engagement Activity 2018
 
Description Interview for Feast Norfolk magazine 
Form Of Engagement Activity A magazine, newsletter or online publication
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Interview for article in Feast Norfolk Magazine aimed at informing the general public about the research happening at Quadram Institute Bioscience.
Year(s) Of Engagement Activity 2019
 
Description Invited member of Irish Department of Agriculture Food and the Marine grant evaluation panel 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Invited to sit as an international expert on an Irish Department of Agriculture Food and the Marine grant evaluation panel.
Call: Food Institutional Research Measure
Year(s) Of Engagement Activity 2020
URL https://www.gov.ie/en/publication/ce553-research/#:~:text=DAFM%20Funded%20Research&text=Food%20Insti...
 
Description Judging panel RSC Emerging Technologies Competition 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Member of Judging Panel for the Royal Society of Chemistry Emerging Technologies competition. Annual competition run by RSC to support start up companies. Food and Beverage panel.
Year(s) Of Engagement Activity 2020
URL https://www.rsc.org/competitions/emerging-technologies/previous-winners/
 
Description Mail on Sunday article on fake foods 
Form Of Engagement Activity A magazine, newsletter or online publication
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact Commented on a range of food myths that the article was trying to get a more balanced scientific view of, including the issue of coconut oil and health.
Year(s) Of Engagement Activity 2018
URL https://www.dailymail.co.uk/health/article-6450123/How-fake-food-news-putting-risk.html
 
Description Meet the Molecules Blog article 
Form Of Engagement Activity Engagement focused website, blog or social media channel
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Media (as a channel to the public)
Results and Impact Meet the Molecules blog article on Resistant Starch
Year(s) Of Engagement Activity 2019
URL https://www.jic.ac.uk/blog/meet-the-molecules-resistant-starch/
 
Description Meeting in London with collaborators from the University of Leicester and Protexin 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Industry/Business
Results and Impact Meeting in London with collaborators from the University of Leicester and Protexin
Year(s) Of Engagement Activity 2018
 
Description Monogram 2018 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact The Monogram Network meeting is the annual get together for the small-grain cereal and grass research community. Academics, commercial scientists and plant breeders meet to hear about the latest advances, exchange ideas and discuss collaboration. We presented a poster on" Digestibility of processed durum wheat with increased resistant starch"
Year(s) Of Engagement Activity 2018
 
Description Mycoprotein blog 
Form Of Engagement Activity Engagement focused website, blog or social media channel
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact Educational blog focussed on recent publications about how mycoprotein influences digestion and promotes health effects
Year(s) Of Engagement Activity 2020
URL https://quadram.ac.uk/how-mycoprotein-influences-digestion-and-promotes-health-effects/
 
Description NIHA Launch 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Postgraduate students
Results and Impact Pecha-Kucha presentation to stimulate interest in the newly launch Norwich Institute for Healthy Ageing, followed by Q&A session.
Year(s) Of Engagement Activity 2020
URL https://www.norwichresearchpark.com/event/launch-norwich-institute-healthy-aging-niha
 
Description Norfolk Spring Fling 2019 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Norfolk Spring Fling is a regional event that welcomes more than 6000 people. We directly interacted with hundreds of them were we explained our research, how our scientific knowledge is being translated to bring healthier food to everyone's table.
Year(s) Of Engagement Activity 2019
URL https://springfling.rnaa.org.uk/
 
Description Norwich Science Festival 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact Norwich Science Festival
Year(s) Of Engagement Activity 2018
 
Description Norwich Science Festival: Clever Food Stall 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact Welcome posters, Research posters, EatWell Guide Poster, Clever Food Trail, Recipe cards, Certificates, Landyards, Stickers, Smelling jars
Year(s) Of Engagement Activity 2019
 
Description PFP Sandpit event 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Postgraduate students
Results and Impact Participated in a sandpit event in which new project ideas were developed. This led to further funding to support an interdisciplinary research project.
Year(s) Of Engagement Activity 2016
 
Description Panel member of the EU JPI PREVNUT Call 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Policymakers/politicians
Results and Impact Invited evaluator and panel member of the EU JPI PREVNUT Call. Joint Programming Initiative 'A Healthy Diet for a Healthy Life'
Evaluated and ranked proposals in the above call and prioritized the top proposals for funding.
Year(s) Of Engagement Activity 2020
URL https://www.healthydietforhealthylife.eu/index.php/call-activities/calls/98-calls-site-restyling/583...
 
Description Presentation 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Undergraduate students
Results and Impact Launch of the NBI Africa Initiative
Year(s) Of Engagement Activity 2020
URL https://www.jic.ac.uk/event/biosciences-innovation-promoting-research-excellence-in-africa/#:~:text=...
 
Description Press Release - New flour brings health power of pulses to our daily bread 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact Press release folllowing on from a peer-reviewed publication. The press release led to further articles in national and international trade journals including 'food manufacture'; 'food navigator', and 'food ingredients first'. This stimulated requests for further infomation from members of the food industry seeking to find out more about the use of this ingredient for various product applications.
Year(s) Of Engagement Activity 2020
URL https://quadram.ac.uk/new-flour-health-pulses-bread/#:~:text=Researchers%20from%20the%20Quadram%20In...
 
Description Product Scoping Workshop 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact 11 people participated in an workshop facilitated by food industry with the aim of developing product concepts for new food ingredients.This led to lots of discussion and thinking about products from consumer points of view.
Year(s) Of Engagement Activity 2019
 
Description Public lecture and Discussion Eaton Summer Programme 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact Public lecture and discussion session as part of the Eaton Summer Programme in Norwich in August 2018. Talk entitled "Are your eyes bigger than your belly? The science behind controlling digestion and reducing appetite"
Aimed to educate the public on the role of food structure on digestion and health outcomes. To inform of the developments with the new Quadram Institute aims and research objectives. Enter into a discussion with the audience regarding the health benefits of certain foods and the role of processing, preparation and structure on nutrient availability.
Year(s) Of Engagement Activity 2018
 
Description Reepham Food Festival - Fun Food Science Activity 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Reepham Food Festival - Fun Food Science Activity
Year(s) Of Engagement Activity 2018
 
Description Reepham Food Festival - Fun Food Science Activity 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact The food festival was made of a range of events, with chef demonstrations, market stalls, schools displays and activities. The Hazard group organised a stand about cereal research and health with hands-on activity.
Year(s) Of Engagement Activity 2018
URL https://twitter.com/MarinaCorrad/status/1000743709397438465
 
Description Royal Norfolk Show 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Royal Norfolk Show. The Royal Norfolk Show is the largest two-day agricultural show in the country. Hosted by the Royal Norfolk Agricultural Association to promote the image, understanding and prosperity of agriculture and the countryside, the show has been part of the fabric of Norfolk since 1847.
Year(s) Of Engagement Activity 2019
URL https://royalnorfolkshow.rnaa.org.uk/
 
Description Royal Norfolk Show 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Royal Norfolk Show
Year(s) Of Engagement Activity 2019
 
Description Soapbox Science 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact Soapbox Science is a science outreach organisation that brings cutting edge research onto urban streets whilst also promoting the visibility of women in science. Speakers stand on soapboxes and engage with the public about their work.
Year(s) Of Engagement Activity 2018
URL http://soapboxscience.org/soapbox-science-2018-norwich/
 
Description Sulforaphane accumulation in the prostate - Results from the 'Norfolk ADaPt' study 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Other audiences
Results and Impact This was a presentation to the Norwich Cancer Research Networks Inaugural symposium. It was a research update and aimed to engage collaborators and practitioners.
Year(s) Of Engagement Activity 2020
URL https://cancerresearchnorwich.org.uk/nrp-cancer-research-symposium-2020/
 
Description The interplay between plant, human and microbial sulphur metabolism in the prevention of aggressive prostate cancer 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact Presentation - The interplay between plant, human and microbial sulphur metabolism in the prevention of aggressive prostate cancer
Year(s) Of Engagement Activity 2018
 
Description The role of 3,4-dihydroxyphenyl-y-valerolactone, the gut microbiota metabolite of (-)-epicatechin, in reducing insulin resistance 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact NuGo Conference, Bern Switzerland 16h-19th September 2019
Year(s) Of Engagement Activity 2019
 
Description The role of 3,4-dihydroxyphenyl-y-valerolactone, the gut microbiota metabolite of (-)-epicatechin, in reducing insulin resistance 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact International conference on polyphenols and health
Year(s) Of Engagement Activity 2019
 
Description Training for placement student from the University of Surrey 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Undergraduate students
Results and Impact Training for placement student from the University of Surrey
Year(s) Of Engagement Activity 2019
 
Description UEA Co-creation Sandpit Event - Innovation, Health and Well-being 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Postgraduate students
Results and Impact The activity aimed to co-create interdisciplinary projects that can only be done through collaboration. There were 70 participants and several teams developed and pitched ideas. Each team involved participants from different background, with a good balance between industry, policy makers, healthcare workers, social scientists, nutritionists etc. Project development and further applications for funding were encouraged. A linked in page has been set up for further networking https://www.linkedin.com/groups/8991208/
After the workshop, some of the new interdisciplinary teams continued to engage and are in the process of applying for further funding to enable the co-created projects to be delivered.
Year(s) Of Engagement Activity 2020
URL https://www.linkedin.com/groups/8991208/
 
Description Visit by Waitrose. 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Industry/Business
Results and Impact To present the research of QIB to representatives of the Waitrose Nutrition and Technology elements.
Year(s) Of Engagement Activity 2019
 
Description Visit from Applied Nutritional Science BSc students from Anglia Ruskin University 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Undergraduate students
Results and Impact Showcase of the research facility resources available and the functionality of the building to provide high standards research facilities. Students were also presented with an overview of the research programmes.
Year(s) Of Engagement Activity 2019
 
Description Visit with Norfolk's Lord-Lieutenant 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Other audiences
Results and Impact Visit by Norfolk's Lord-Lieutenant to discuss research at the John Innes Centre and Quadram Institute BIoscience
Year(s) Of Engagement Activity 2019
 
Description WUR students visit 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact 30 students from Wageningen University visited the Quadram Institute.
This is part of an European excursion organised by Wageningen Univeristy, in The Netherlands. At the end of the third and final year of the bachelor's or in the first year of the master's the students may choose for participating in the yearly organized European Excursion, a 7-10 days excursion through 1-4 European countries, during which visits are scheduled to universities, nutrition and health institutes, food and nutrition industries, non-governmental health. The excursion aims to cover all different specializations within the field of nutrition and health.
Year(s) Of Engagement Activity 2020
 
Description Wageningen European Study trip 2020 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Undergraduate students
Results and Impact A series of presentations and tours for these undergraduate/masters students of nutrition and health.
Year(s) Of Engagement Activity 2020
 
Description Wageningen European Study trip 2020 A series of presentations and tours for these undergraduate/masters students of nutrition and health. 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Undergraduate students
Results and Impact A series of presentations and tours for these undergraduate/masters students of nutrition and health.
Year(s) Of Engagement Activity 2020
 
Description Westminster Health Forum Keynote Seminar: Improving diabetes outcomes in England - prevention, access and digital technology 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Policymakers/politicians
Results and Impact This seminar focused on next steps for improving diabetes outcomes in England.
Delegates discussed priorities for moving forward with the NHS Long Term Plan for diabetes - including workforce training and funding challenges associated with the Plan's goals to improve access to diabetes professionals, such as multidisciplinary footcare teams and diabetes inpatient specialist nursing teams.
Year(s) Of Engagement Activity 2019
URL https://www.westminsterforumprojects.co.uk/publication/improving-diabetes-outcomes-19
 
Description Women for the future conference 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Schools
Results and Impact Women of the Future is a one day conference for Year 10 girls hosted by the John Innes Centre, designed to educate, advise and inspire. I was involved as a STEMM professionals and as a woman to tell my story, my research experience and what lead me to chose this career path.
Year(s) Of Engagement Activity 2018,2019
URL https://www.jic.ac.uk/training-careers/work-experience/women-of-the-future/
 
Description Women of the Future Conference 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact This event for ~250 Year 10 female students aimed to inspire the students into studying and developing careers in science through meeting scientists
Year(s) Of Engagement Activity 2019
 
Description Year 10 UEA Summer School Protein Workshop 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact This session enabled students to understand the concept of protein biochemistry and basic bioinformatics by use of online free available software.
Year(s) Of Engagement Activity 2019
 
Description You tube video Food structure, colloid and digestion 
Form Of Engagement Activity A broadcast e.g. TV/radio/film/podcast (other than news/press)
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Media (as a channel to the public)
Results and Impact You tube video describing the research done in the group to inform the wider public about the scope and impacts of our research and also to provide information to industry and other stakeholders who may want to collaborate
Year(s) Of Engagement Activity 2018
URL https://www.youtube.com/watch?v=e7aMpnps4-Q
 
Description Youth STEMM Conference 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Schools
Results and Impact Youth STEMM Conference is a one day conference for young people ranging from 13 to 18 years old and coming from schools across Norfolk and Suffolk. I was involved as a STEMM professionals to share my research experience and what lead me to chose this career path.
Year(s) Of Engagement Activity 2018
URL https://www.jic.ac.uk/blog/youth-stemm-award-mid-term-conference/
 
Description iTeams project 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Postgraduate students
Results and Impact A team of five university students are using academc research outputs to develop commercial viable startegies and build industry-university links under the mentorship of an expert. The activity is currently in progress.
Year(s) Of Engagement Activity 2021
URL https://iteamsonline.org/
 
Description speed networking event 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact We attended a speed networking event for pupils in year 12 at Wymondham Sixth Form College. This involved talking about our career trajectories, what we do as scientists on a day to day basis and importantly the important work being carried out here at the Quadram Institute. Students were keen to hear more about the interaction of food and the gut microbiota and their influence to health.
Year(s) Of Engagement Activity 2019