Porcine Reproductive and Respiratory Syndrome (PRRS) Immunology

Lead Research Organisation: The Pirbright Institute
Department Name: UNLISTED

Abstract

Porcine reproductive and respiratory syndrome (PRRS) is one of the most important pig diseases worldwide with a huge economic impact, estimated in the USA alone to exceed $600 million p.a. PRRS virus (PRRSV) is an arterivirus which exists in two distinct genotypes. Due to the replication cycle being prone to mutation and recombination events, PRRSV is a rapidly evolving virus, which is most dramatically illustrated by the emergence of highly pathogenic variants in South East Asia and Eastern Europe. Thus, more efficacious control strategies are urgently sought. An improved understanding of immunology and pathology of PRRS will aid the development of the next generation of PRRSV vaccines or intervention strategies. To address this need we aim to:
1. Characterise the interactions of PRRSV with dendritic cells
2. Define PRRSV virulence determinants and their mechanisms of action
3. Elucidate the roles of neutralising antibodies and T cell responses in protective immunity
4. Develop and evaluate novel PRRSV vaccines

Publications

10 25 50

 
Description We have developed methodologies to identify and isolate antibody-secreting and memory B lymphocytes from pigs.
Exploitation Route These techniques can be utilized to study these cells and their antibodies from infected/immunized pigs, which can play an important role in the development of improved vaccines for PRRS and other diseases.
Sectors Agriculture, Food and Drink,Pharmaceuticals and Medical Biotechnology

 
Description BBSRC Industrial CASE (iCASE) studentship programme Oxford Interdisciplinary Bioscience Doctoral Training Partnership
Amount £80,000 (GBP)
Organisation University of Oxford 
Sector Academic/University
Country United Kingdom
Start 10/2019 
End 09/2023
 
Description European PRRS Research Award
Amount € 25,000 (EUR)
Organisation Boehringer Ingelheim 
Sector Private
Country Germany
Start 11/2016 
End 10/2017
 
Description The Pirbright Institute PhD Studentship
Amount £80,000 (GBP)
Organisation The Pirbright Institute 
Sector Academic/University
Country United Kingdom
Start 10/2017 
End 10/2021
 
Description Evaluation of artificial cell membranes for delivery of PRRSV antigens 
Organisation ACM Biolabs
PI Contribution Ability to evaluate the immunogenicity of artificial cell membranes (ACMs) for delivery of PRRSV antigens
Collaborator Contribution To use their patented platform technology to produce a panel of ACMs embedded with different PRRSV envelope proteins
Impact Not yet
Start Year 2017
 
Description Evaluation of porcine cytomegalovirus as a viral vaccine vector 
Organisation University of Plymouth
Country United Kingdom 
Sector Academic/University 
PI Contribution PRRSV model and vaccine candidate antigens. Expertise in porcine T cell immunology
Collaborator Contribution Expertise in exploiting herpesviruses as vaccine vectors
Impact not yet
Start Year 2015
 
Description Evaluation of the efficacy of antiviral nanoparticles (AVNPs) against PRRSV 
Organisation University of Hertfordshire
Country United Kingdom 
Sector Academic/University 
PI Contribution Expertise in PRRSV and in vitro model to assess efficacy of AVNPs
Collaborator Contribution Expertise in materials science
Impact Not yet
Start Year 2016
 
Description Evaluation of virus-imprinted polymers as synthetic neutralisng antibodies 
Organisation University of Central Lancashire
Country United Kingdom 
Sector Academic/University 
PI Contribution Model viruses and systems to evaluate the ability of molecularly-imprinted polymers to neutralise virus infectivity
Collaborator Contribution Expertise in molecularly-imprinted polymers
Impact Wellcome Trust Seed Award
Start Year 2015
 
Description Nanoparticulate antigen delivery systems 
Organisation University College London Hospital
Country United Kingdom 
Sector Public 
PI Contribution Expertise in disease models, vaccine antigens and immune response evaluation
Collaborator Contribution Expertise in nanoparticulate formulations
Impact Several joint publications
Start Year 2012
 
Description Novel PRRS and swine influenza vaccines 
Organisation National Center for Genetic Engineering and Biotechnology (BIOTEC)
Country Thailand 
Sector Public 
PI Contribution Expertise in PRRS immunology and vaccine evaluation.
Collaborator Contribution Novel vaccine strains and SE Asian field strains
Impact Collaborative grant proposal funded under the BBSRC Newton Fund UK-China-Philippines-Thailand Swine and Poultry Research Initiative
Start Year 2017
 
Description Provision of PRRSV field strains 
Organisation Animal and Plant Health Agency
Country United Kingdom 
Sector Public 
PI Contribution Experimental heterologous PRRSV challenge model to assess the induction of broadly neutralizing antibodies. Model to assess dendritic cell tropism of PRRS viruses
Collaborator Contribution Provision of PRRSV-1 and -2 field strains
Impact Not yet
Start Year 2016
 
Description Provision of PRRSV field strains 
Organisation Kansas State University
Country United States 
Sector Academic/University 
PI Contribution Experimental heterologous PRRSV challenge model to assess the induction of broadly neutralizing antibodies. Model to assess dendritic cell tropism of PRRS viruses
Collaborator Contribution Provision of PRRSV-1 and -2 field strains
Impact Not yet
Start Year 2016
 
Description Reporter PRRS viruses 
Organisation Kansas State University
Country United States 
Sector Academic/University 
PI Contribution A new application for recombinant reporter PRRS viruses i.e. to use them as a high throughput screen for neutralizing (monoclonal) antibodies. Use as tools to assess the tropism of PRRSV for dendritic cell subsets and the functional consequences.
Collaborator Contribution Provision of plasmids encoding GFP-expressing PRRSV-1 and -2.
Impact Not yet
Start Year 2016
 
Description Retroviral-mediated genetic programming of porcine memory B cells 
Organisation AIMM Therapeutics
PI Contribution Provided a novel 'model' system to test their technology to isolate novel monoclonal antibodies
Collaborator Contribution To provide their patented platform technology to genetically program porcine memory B cells to enable the isolation of memory B cells
Impact Not yet
Start Year 2016
 
Description Use of fluorescent virions to isolate PRRSV-specific porcine B cells 
Organisation University of Minnesota
Country United States 
Sector Academic/University 
PI Contribution Deployment of a novel technology to facilitate isolation of PRRSV neutralizing monoclonal antibodies
Collaborator Contribution Provision of a method to fluorescently tag purified virions to label PRRSV-specific B cells
Impact None to report to date
Start Year 2016
 
Description Invited Presentation 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact Presentations at the European PRRS Research Award ceremonies
Year(s) Of Engagement Activity 2016,2017
 
Description Invited Presentation 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact Invited talk entitled 'Identification of viral targets of host T cell immunity for development of livestock vaccines' presented at the International Symposium on Human and Veterinary Viruses 2018: From Research to Application, Bangkok, Thailand.
Year(s) Of Engagement Activity 2018
URL http://www.biotec.or.th/virus-symposium2018/index.php
 
Description Invited Presentation 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Invited talk titled 'Identification of viral targets of host T cell immunity for development of livestock vaccines' presented at the British Society for Immunology Congress, Brighton, UK.
Year(s) Of Engagement Activity 2010,2017
URL https://www.bsicongress.com/
 
Description Invited Presentation 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Invited talk titled 'Design and evaluation of biomimetic particulate formulations of viral antigens as veterinary vaccines' presented at VaxInLive 2018 Symposium, Lyon, France.
Year(s) Of Engagement Activity 2018
URL https://clarolineconnect.univ-lyon1.fr/icap_website/view/1228
 
Description Invited oral presentation at 18th National Conference for Pig Disease Prevention and Eradication, Wuhan, China 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Invited oral presentation: 'Trying to hit a moving target: Novel approaches to porcine reproductive and respiratory syndrome virus vaccine development', 18th National Conference for Pig Disease Prevention and Eradication, Wuhan, China
Year(s) Of Engagement Activity 2018
 
Description Invited presentation: 'Beyond Jenner: The Future of Vaccines', Discovery Day, Dr Jenner House Museum and Gardens 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Invited by the British Society for Immunology to give a presentation on vaccine research at the 'Beyond Jenner: The Future of Vaccines', Discovery Day, Dr Jenner House Museum and Gardens, Berkeley, UK.
Year(s) Of Engagement Activity 2018
 
Description Oral presentation at International Pig Veterinary Symposium and International PRRS Symposium, Chongqing, China. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Oral presentation on our PRRS research at the International Pig Veterinary Symposium and International PRRS Symposium, Chongqing, China.
Year(s) Of Engagement Activity 2018
 
Description University of Surrey School of Veterinary Medicine Open Day 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Presentation of work conducted at The Pirbright Institute
Year(s) Of Engagement Activity 2016,2017
 
Description Vet School Open Day 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Schools
Results and Impact University of Surrey School of Veterinary Medicine Open Day - introduced The Pirbright Institute and the research we conduct to prospective students and parents.
Year(s) Of Engagement Activity 2018