Viral Persistence
Lead Research Organisation:
The Pirbright Institute
Department Name: UNLISTED
Abstract
There is no single mechanism adopted by viruses to establish a persistent infection, and the propensity for persistence can vary between closely related viral serotypes. Therefore, studying the natural hosts has enormous advantages over model species for dissecting the detailed molecular processes and pathways involved. The suite of biological mechanisms leading to persistence can be complex and the outcome of infection can vary depending on minor variation in the virus, host or indeed other environmental influences. Consequently insights into the entire process of viral pathogenesis leading to persistence is crucial to both identify targets for disease control and in making sure that any interventions are not likely to exacerbate disease. These studies build on the established, integrated and systems approach to animal infection studies that is a unique strength of the work undertaken at Pirbright. The studies in this topic is particularly closely linked to the ‘recognition and control of virus infections’ topic where the host innate and adaptive immune responses have failed to clear an infection.
Marek’s disease virus (MDV) has developed multiple mechanisms for evading clearance by the host for and persisting. Avian hosts have adapted by initiating a balanced immune response that does not cause untoward tissue damage, but restricts virus replication to tolerable levels. Understanding this dynamic equilibrium is challenging, but very important to develop innovative intervention control strategies. African buffalo can become persistently infected with foot and mouth disease virus (FMDV) creating a virus reservoir that influences viral evolution and transmission to cattle. Understanding the process of persistence will be an important component of any future eradication programme in Africa and could identify opportunities to control this disease in cattle. Porcine respiratory and reproductive syndrome virus (PRRSV) strains are poor inducers of type I interferon (IFN) both in vitro and in vivo. PRRSV antagonises the production of type I IFN by infected cells in order to facilitate viral replication and persistence. Indeed, replication of the virus in porcine alveolar macrophages can be controlled by IFN-a treatment.
Marek’s disease virus (MDV) has developed multiple mechanisms for evading clearance by the host for and persisting. Avian hosts have adapted by initiating a balanced immune response that does not cause untoward tissue damage, but restricts virus replication to tolerable levels. Understanding this dynamic equilibrium is challenging, but very important to develop innovative intervention control strategies. African buffalo can become persistently infected with foot and mouth disease virus (FMDV) creating a virus reservoir that influences viral evolution and transmission to cattle. Understanding the process of persistence will be an important component of any future eradication programme in Africa and could identify opportunities to control this disease in cattle. Porcine respiratory and reproductive syndrome virus (PRRSV) strains are poor inducers of type I interferon (IFN) both in vitro and in vivo. PRRSV antagonises the production of type I IFN by infected cells in order to facilitate viral replication and persistence. Indeed, replication of the virus in porcine alveolar macrophages can be controlled by IFN-a treatment.
Publications
Baron MD
(2018)
Recent advances in viral vectors in veterinary vaccinology.
in Current opinion in virology
Bondada MS
(2019)
Multifunctional miR-155 Pathway in Avian Oncogenic Virus-Induced Neoplastic Diseases.
in Non-coding RNA
Boodhoo N
(2019)
Replication of Marek's Disease Virus Is Dependent on Synthesis of Fatty Acid and Prostaglandin E.
in Journal of virology
Chang P
(2019)
Amino Acid Residue 217 in the Hemagglutinin Glycoprotein Is a Key Mediator of Avian Influenza H7N9 Virus Antigenicity.
in Journal of virology
Chen Y
(2017)
PD-L1/PD-1 Pathway Blockade Reverses Dysfunction of Circulating CEA-Specific T Cells in Pancreatic Cancer Patients.
in Clinical cancer research : an official journal of the American Association for Cancer Research
Ferretti L
(2018)
Within-Host Recombination in the Foot-and-Mouth Disease Virus Genome.
in Viruses
| Description | The key findings from the studies on persistent infections of three viruses viz. Marek's disease virus (MDV) in poultry, Porcine respiratory and reproductive syndrome virus (PRRSV) in pigs and Foot and mouth disease virus (FMDV) in African buffaloes (Syncerus caffer). 1. MDV latency in tumour cell lines is maintained by epigenetic mechanisms: During latency MDV expresses only a limited set of genes which are kept transcriptionally active with boundaries maintained using specific insulator sequences. We have determined global changes in the methylation of the viral/host promoters by determining the first chicken global DNA methylome of oncogenic virus-transformed cancer cells. 2. CRISPR/Cas9-based in situ gene editing for examining the determinants of MDV persistence: We have developed CRISPR/Cas9-based gene editing tools in situ in MDV-transformed cancer cell lines to identify the determinants that contribute the maintenance of latency as well as that help in the reactivation of MDV to lytic replication. Using this approach, we have shown that MDV-encoded pp38, is not essential or a determinant of latency-to-lytic switch. 3. COX-2-PGE2 pathway is important in MDV persistence: Activation of COX-2/PGE2 pathway, essential for MDV replication, can modulate both innate and adaptive immunity against MDV replication, influencing latency. 4. Follicular dendritic cells in the germinal centre are important in FMDV persistence and immune responses in mouse infection model: Persistence of FMDV in the lymphoid tissues of ruminants has been localised to follicular dendritic cells (FDCs) that reside in the light zone of the germinal centre (GC). FDCs were very important in persistence as FMDV copy numbers decreased significantly when they were depleted with recombinant lymphotoxin beta receptor immunoglobulin fusion protein (LTßR-Ig). Moreover, LTß-deficient mice that lacked FDC and normal intact splenic marginal zone, failed to trap and retain FMDV in the GC, failed to generate neutralizing antibodies to FMDV and showed impaired FMDV-specific humoral immune responses. 5. African buffaloes are not the major source of new FMDV variants: African buffaloes (Syncerus caffer) are efficient carriers of FMDV and it has been proposed that new virus variants are produced in carrier buffalo may spread to cause disease in livestock populations. We have shown that despite an accumulation of low frequency sequence variants over time, there is no evidence of significant antigenic variation, suggesting that carrier buffalo are unlikely to be a major source of new variants. Our studies also showed that antibody escape does not contribute to the persistent infections in buffaloes. 6. PRRSV non-structural proteins show multiple interactions with host proteins: Using yeast-two-hybrid screens with PRRSV NSP1 and a porcine alveolar macrophage library, we have identified a number of putative interactions including those associated with IFN signalling, the NF-?B pathway, ubiquitination and nuclear transport, suggesting their roles in pathogenesis and persistence of PRRSV. |
| Exploitation Route | 1. MDV immune responses: Our studies demonstrating the IFN-gamma-producing cells indicated their role in the protection against MD, showing their potential for vaccine development. Immunological assays on T cell responses could be utilized to study T cell responses to other infections in chickens. Similarly, identification of the COX-2/PGE2 pathway in MD pathogenesis could be explored in developing novel control methods. 2. CRISPR/Cas9-based gene editing for studying MDV biology: Development of these technologies will help in better understanding of virus-host interactions and development of recombinant vaccines. 3. Identification of the role of FDCs in persistence of FMDV in mouse model: We have utilized a mouse monoclonal antibody (mAb 4B2) raised against mouse complement receptor (CR) 2/CR1 to block these receptors on FDC in BALB/C mice infected with FMDV/A/Arg/1/2000. In pilot studies, we have demonstrated that mAB 4B2 can block the binding of FMDV to FDC demonstrating a specific interaction between the virus and FDC receptors. These studies will help in the design of vaccines to induce long-term immunity and understand the role of "carrier" animals in the maintenance and spread of infection in wildlife and livestock populations. |
| Sectors | Agriculture, Food and Drink |
| Description | Contributing to the chapter on Marek's disease for the OIE Terrestrial Manual |
| Geographic Reach | Multiple continents/international |
| Policy Influence Type | Implementation circular/rapid advice/letter to e.g. Ministry of Health |
| Impact | The contribution to the Terrestrial Manual on the recent advances in the diagnosis and control of Marek's disease will impact on improved and better control of the disease to reduce losses and improved food security |
| URL | http://www.oie.int/ |
| Description | Organised seminar series on Sequencing, Virus Evolution and Molecular Biology at Pirbright (with Luca Ferretti and Graham Freimanis) |
| Geographic Reach | Local/Municipal/Regional |
| Policy Influence Type | Influenced training of practitioners or researchers |
| Description | Participation in the Management of the Oxford DTP |
| Geographic Reach | Multiple continents/international |
| Policy Influence Type | Influenced training of practitioners or researchers |
| Impact | As part of the Management committee of the Oxford-Pirbright-Oxford Brookes-Diamond doctorate Training Partnership, contributed to the success of the DTP in meeting the objectives, recruitment and review |
| URL | http://www.biodtp.ox.ac.uk/ |
| Description | Taught course on introductory bioinformatics to PhD students at Pirbright |
| Geographic Reach | Local/Municipal/Regional |
| Policy Influence Type | Influenced training of practitioners or researchers |
| Description | Dissecting the molecular pathways of MDV oncoprotein Meq for understanding pathogenesis and aid vaccine development |
| Amount | £440,000 (GBP) |
| Funding ID | BB/R007896/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 04/2018 |
| End | 03/2021 |
| Description | Microbiology Society Travel Grant |
| Amount | £233 (GBP) |
| Organisation | Microbiology Society |
| Sector | Learned Society |
| Country | Unknown |
| Start | 04/2018 |
| End | 04/2018 |
| Description | The Pirbright Institute PhD Studentship |
| Amount | £80,000 (GBP) |
| Organisation | The Pirbright Institute |
| Sector | Academic/University |
| Country | United Kingdom |
| Start | 10/2017 |
| End | 10/2021 |
| Description | UK-China partnership on Global Food Security: Combating avian tumor diseases for sustainable poultry proudction |
| Amount | £382,000 (GBP) |
| Funding ID | BB/R007632/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 04/2018 |
| End | 03/2020 |
| Title | CRISPR/Cas9-based gene editing of avian herpesvirus vectors to develop novel recombinant vaccines |
| Description | Development of recombinant herpesvirus vaccines are usually achieved by classical homologous recombination methods. The advent of new genome editing tools has enabled its application on avian herpesvirus vaccine strains to develop new recombinant vaccines potentially capable of expressing protective antigens from multiple pathogens |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2017 |
| Provided To Others? | Yes |
| Impact | The major impact will be in the generation of novel vaccines that can simultaneously protect against multiple avian diseases |
| URL | http://www.poultryworld.net/Health/Articles/2016/12/Is-GM-technology-the-future-of-poultry-vaccines-... |
| Title | Generation of feather follicle stem cells |
| Description | Methods for generation and expansion of feather follicle epithelial stem cells are developed within Avian Immunology group. |
| Type Of Material | Cell line |
| Year Produced | 2018 |
| Provided To Others? | No |
| Impact | Feather follicle stem cells can be used to examine Marek's disease virus replication in vitro. |
| Title | FMDV LIMS and webtools |
| Description | The Integrative Biology and Bioinformatics (IBB) group has been supplying state-of-the-art bioinformatics tools to the World Reference Labs for the Foot-and-Mouth Disease Virus. All tools revolve around a database of genomic sequences and metadata for FMDV samples. Some tools allow members of the lab to enter, process and annotate new data into the database, generating several kinds of reports. Other web-based tools (the "FMDV Toolbox") allow external users to query the database in several ways. Bioinformatics workflows are hosted on the IBB cluster, and have been implemented as REST web services so as to be decoupled from the web interface. The web interface is currently being redeveloped with the help of a specialised company. |
| Type Of Material | Database/Collection of data |
| Year Produced | 2016 |
| Provided To Others? | Yes |
| Impact | The reports generated by our system are used to inform FMDV policy-makers both within the UK and in the rest of the world. External users can compare their sequences to those contained in the database, and get useful biological insights. |
| URL | https://mallorn.pirbright.ac.uk/FMDVToolbox |
| Title | Genome browser for MDV infected chicken cells |
| Description | The database integrates and makes available the results of the bioinformatics analysis of several experiments involving high-throughput sequencing of chicken cells infected by several viruses (mainly Marek's Disease Virus) generated within the Avian Viral Disease group at the Pirbright Institute. |
| Type Of Material | Database/Collection of data |
| Year Produced | 2015 |
| Provided To Others? | Yes |
| Impact | The genome browser allows the researchers to meaningfully mine and integrate the large amount of data produced by their experiments, guiding subsequent experimental hypothesis-driven validation in the lab. |
| Title | Viral assembly pipeline |
| Description | The Integrative Biology and Bioinformatics group has developed a data analysis pipeline to obtain sensitive and robust viral assemblies out of high-throughput sequencing data. In spite of the relatively short genomic length of most viruses, assembling of viral sequences can be challenging due to several reasons, such as: low amount of material in the sample, which might require amplification, introducing biases, and/or generates low-quality sequences; uneven coverage due to low-quality material or the genomic material of the virus being RNA; the viral nucleic acid being almost lost in the background of nucleic acid of the host. Our pipeline overcomes most of those problems, and is even able to detect different viral strains being present together in the same sample. It can also be used on metagenomic environmental samples. |
| Type Of Material | Data analysis technique |
| Year Produced | 2017 |
| Provided To Others? | No |
| Impact | The pipeline is being deployed as the tool of choice for the nascent sequencing facility at Pirbright. It has been used by several groups at Pirbright, and the FMDV World Reference Lab, in order to assemble very different kinds of viruses. |
| Title | Viral variant calling pipeline |
| Description | The Integrative Biology and Bioinformatics group has developed a data analysis pipeline to obtain sensitive and robust variant calling for viral sequences out of high-throughput sequencing data. In spite of the relatively short genomic length of most viruses, calling variant for viral sequences can be challenging due to several reasons, such as: low amount of material in the sample, which might require amplification, introducing biases, and/or generates low-quality sequences; very high genome coverage, which slows down most existing variant callers; the presence of a potentially very high number of different haplotypes for RNA viruses, which is an unusual scenario in variant calling. Our fast and sensitive Bayesian pipeline overcomes most of those problems. |
| Type Of Material | Data analysis technique |
| Year Produced | 2017 |
| Provided To Others? | No |
| Impact | The pipeline is being deployed as the tool of choice for the nascent sequencing facility at Pirbright. It has been used by several groups at Pirbright, and the FMDV World Reference Lab, in order to assemble very different kinds of viruses. |
| Description | Identification and functional characterisation of host proteins that interact with non-structural proteins of PRRSV |
| Organisation | University of Liverpool |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Expertise in PRRSV |
| Collaborator Contribution | Expertise in viral-host protein interactions |
| Impact | Not yet |
| Start Year | 2017 |
| Description | Professor Sahyan Sharif, University of Guelph, Canada |
| Organisation | University of Guelph |
| Department | Department of Pathobiology |
| Country | Canada |
| Sector | Academic/University |
| PI Contribution | Collaboration between my team and Professor Sharif lead to new finding in Avian immunology, specifically in understanding the effects of nutrients on antigen presenting cells and T cells of chicken. We took the lead in studying the effects of some nutrients (Vitamin D) on the function of chicken T cells, performed most of the experiments in our laboratory. Meanwhile, we participated in studying the effects of Vitamin D on antigen presenting cells of chicken by performing some key experiments because we had access to some specific reagents (monoclonal antibodies) recognizing molecules expressed on chicken antigen presenting cells. |
| Collaborator Contribution | Professor Sharif team took the lead in studying the effects of Vitamin D on antigen presenting cells of chicken, and contributed to experimental plan for studying the effects on chicken T cells. |
| Impact | Two publications in 2015 and 2016: 1. Nitish Boodhoo, Shayan Sharif, Shahriar Behboudi. 1a,25(OH)2 Vitamin D3 Modulates Avian T Lymphocyte Functions without Inducing CTL Unresponsiveness. PLoS One. 2016 Feb 24;11(2):e0150134 2. Bahram Shojadoost, Shahriar Behboudi, Villanueva AI, Jennifer Brisbin, Ali Ashkar, Shayan Sharif. Vitamin D3 modulates the function of chicken macrophages. Res. Vet. Sci. 2015 June, 100: 45-51 |
| Start Year | 2014 |
| Description | Re-annotation of Marek's Disease Virus |
| Organisation | U.S. Department of Agriculture USDA |
| Department | Beltsville Agricultural Research Center |
| Country | United States |
| Sector | Academic/University |
| PI Contribution | We are using bioinformatics analysis of high-throughput sequencing data in order to provide a better annotation of the genome of Marek's Disease Virus |
| Collaborator Contribution | Our partners are sharing their extensive expertise of the genomics of MDV, and performing experimental validations |
| Impact | No output yet |
| Start Year | 2015 |
| Description | Poster presentation: International Pig Veterinary Symposium and International PRRS Symposium, Chongqing, PR China |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Luke Johnson (PhD student) gave a poster presentation on his studies on the interaction of macrophages and dendritic cells with PRRSV of differing virulence. |
| Year(s) Of Engagement Activity | 2018 |
| Description | CABI_Research activity of Avian Immunology Group at The Pirbright Institute |
| Form Of Engagement Activity | Engagement focused website, blog or social media channel |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Media (as a channel to the public) |
| Results and Impact | Release of research activity within Avian Immunology group at The Pirbright Institute |
| Year(s) Of Engagement Activity | 2018 |
| Description | Chaired the Poultry Disease group meeting |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | Poultry Disease group is a forum of veterinary practitioners to discuss the advances in poultry diseases that could be translated from research labs to the farms |
| Year(s) Of Engagement Activity | 2017 |
| Description | Conor Haydon participated in the STEM Day - Speed interviews and Careers Day |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Schools |
| Results and Impact | STEM day engagement and careers |
| Year(s) Of Engagement Activity | 2018 |
| Description | Diamond open day |
| Form Of Engagement Activity | Participation in an open day or visit at my research institution |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Public/other audiences |
| Results and Impact | Over 300 people visited our stand at the open day, in which we described the importance of our work for food security and public health. Children from all ages were involved and learned about viruses and played with the huge virus particle that we had produced. One of the major impact was that we described to several people why we work on some viruses that we do not have them in the UK, and the importance of the work for the UK and UK economy and public health was explained. |
| Year(s) Of Engagement Activity | 2017 |
| URL | http://www.diamond.ac.uk/Public/VisitUs.html |
| Description | EUFMD 2016 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Policymakers/politicians |
| Results and Impact | During the EuFMDV conference taking place from 26 to 28 October 2016, Paolo Ribeca gave a talk on "VIBAsys and FMDV-Tools: Practical resources for FMDV sequence analysis". The conference, organised by the EU, gathers most of the important actors in FMD control. The talk explained how the new resources being developed by the Integrative Biology and Bioinformatics on behalf of the FMDV World Reference Lab can be useful to the community |
| Year(s) Of Engagement Activity | 2016 |
| Description | Inauguration of the UK-China Centre of Excellence for Research on Avian Diseases (CERAD) and Symposium on Recent Advances in Avian Disease Research |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Inauguration of the UK-China Centre of Excellence for Research on Avian Diseases (CERAD) and Symposium on Recent Advances in Avian Disease Research. This was held in Binzhou, Shandong province in China in 2015 |
| Year(s) Of Engagement Activity | 2015 |
| Description | Invitation to give keynote talk on 'Control of avian diseases: Challenges and opportunities' |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Gave the Keynote talk on 'Control of avian diseases: Challenges and opportunities' at this International Symposium organized with an aim to gather international virology experts from both human and veterinary fields to share research experience, update findings and contribute their expertise through presentation and interactive discussion. The topics will cover a wide range of virology research common in both human and animal aspects. In addition, technological platforms such as vaccine development, and novel assays will be emphasized and addressed. |
| Year(s) Of Engagement Activity | 2018 |
| URL | http://www.biotec.or.th/virus-symposium2018/index.php |
| Description | Invitation to talk at the Vaccine Symposium "Vaccines at different levels of complexity from subunit to whole cell formulations" jointly organised by the Centre of Genomic Regulation (CRG), Radboud UMC and MSD Animal Health, with the support from the MycoSynVac project |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Gave a talk and participated in the discussion on the challenges and research opportunities for innovation in poultry disease control |
| Year(s) Of Engagement Activity | 2017 |
| URL | http://www.crg.eu/ |
| Description | Invited oral presentation at 18th National Conference for Pig Disease Prevention and Eradication, Wuhan, China |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | Invited oral presentation: 'Trying to hit a moving target: Novel approaches to porcine reproductive and respiratory syndrome virus vaccine development', 18th National Conference for Pig Disease Prevention and Eradication, Wuhan, China |
| Year(s) Of Engagement Activity | 2018 |
| Description | Invited talk at the National Institute for Animal Biotechnology, Hyderabad on 3rd Jan 2019 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Professional Practitioners |
| Results and Impact | Participants were engaged in the recent advances in biotechnology, challenges and opportunities. |
| Year(s) Of Engagement Activity | 2019 |
| Description | Invited to talk at the International Conference on Building Human and Animal Health Capacities organised by the Jordan University of Science and Technology |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Participated in the discussion and talked on 'The recent advances in vaccination-based control of poultry diseases'. The need for increased awareness of the pathogen diversity in the design of vaccines and the opportunities from advances in vaccine technologies were discussed |
| Year(s) Of Engagement Activity | 2017 |
| URL | http://www.just.edu.jo/conferences/bhahc/Pages/default.aspx |
| Description | Keynote talk at the World Veterinary Poultry Association meeting |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Invited to give the keynote talk on 'Avian tumour viruses: their contribution to our understanding of cancer' at the World Veterinary Poultry Association XXth Congress in Edinburgh |
| Year(s) Of Engagement Activity | 2017 |
| URL | http://www.wvpac2017.com/ |
| Description | MDV conference Tours 2016 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Other audiences |
| Results and Impact | During the 11th International Symposium on Marek's Disease and Avian Herpesviruses, which took place from 6 to 9 July 2016, Paolo Ribeca gave a talk on "Attenuation of MDV: an RNA-seq based perspective". |
| Year(s) Of Engagement Activity | 2016 |
| Description | Organised the 3rd UK-China CERAD meeting in Guildford, UK in July 2017 |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | The 3rd UK-China CERAD meeting helped to bring together professional from both countries to identify challenges and exploit the opportunities for improved control of poultry diseases and increasing food security. |
| Year(s) Of Engagement Activity | 2017 |
| URL | http://www.uk-china-cerad.org/ |
| Description | Organising and giving Keynote talk at the GARAD meeting |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Global Alliance of Poultry industry professional and academia to discuss about the advances in control strategies of diseases |
| Year(s) Of Engagement Activity | 2018 |
| URL | http://Garad.org |
| Description | Poster presentation: European Veterinary Immunology Workshop, Utrecht, The Netherlands |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Luke Johnson (PhD student) gave a poster presentation on his studies on the interaction of macrophages and dendritic cells with PRRSV of differing virulence. |
| Year(s) Of Engagement Activity | 2018 |
| Description | Presentation on the effects on oncogenic virus infection at the Veterinarian's Forum, Namakkal, India on 29 Dec 2018 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Professional Practitioners |
| Results and Impact | The purpose of the engagement was to update the practicing poultry veterinarians on the problems with oncogenic virus diseases and methods of control. The meeting attended by nearly 100 veterinarians was very informative and ended with a long discussion on the challenges and innovations |
| Year(s) Of Engagement Activity | 2018 |
| Description | School Year 9 STEM Networking Event, 2018 |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | School Year 9 STEM Networking Event, 11th May, 2018 |
| Year(s) Of Engagement Activity | 2018 |
| Description | Sue Baigent participated in the Teen Tech event on 22nd Jan 2019 on General Science engagement |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | A General Science event |
| Year(s) Of Engagement Activity | 2019 |
| Description | Sue Baigent participated in the Winston Churchill Careers Fair on 7th Nov 2018 |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | Careers fair |
| Year(s) Of Engagement Activity | 2018 |
| Description | Surrey Open day |
| Form Of Engagement Activity | Participation in an open day or visit at my research institution |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Undergraduate students |
| Results and Impact | Described the research activity of the Pirbright Institute for students from school, graduate and postgraduate students, and provided information on research facilities in the institute. |
| Year(s) Of Engagement Activity | 2017 |
| URL | https://www.surrey.ac.uk/opendays/vet |
| Description | The Microbiology Society Annual Conference, Birmingham, 2018 |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | The Microbiology Society Annual Conference, Birmingham, 2018 |
| Year(s) Of Engagement Activity | 2018 |
| Description | UK-India bioinformatics workshop |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Postgraduate students |
| Results and Impact | On February 9 2018 Pirbright organised a "UK-India bioinformatics workshop" in order to foster ties between Indian and UK bioinformaticians working in the field of livestock and crop research. Paolo Ribeca gave a talk on "Bioinformatics and sequencing at Pirbright". |
| Year(s) Of Engagement Activity | 2018 |
| Description | University of Liverpool IGH Day, 16th November 2017 |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Professional Practitioners |
| Results and Impact | University of Liverpool IGH Day, 16th November 2017 |
| Year(s) Of Engagement Activity | 2017 |
| Description | University of Surrey School of Veterinary Medicine Open Day |
| Form Of Engagement Activity | Participation in an open day or visit at my research institution |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Public/other audiences |
| Results and Impact | Presentation of work conducted at The Pirbright Institute |
| Year(s) Of Engagement Activity | 2016,2017 |
| Description | V Nair invited to talk on 'Cell type- and disease stage-specific interactions of Marek's disease virus & the host' at the International Symposium on Marek's disease and avain herpesiviruses at Yangzhou, China during 29th July to 2nd Aug 2018 |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Most recent updates in the field of avian herpesvirus research was discussed and active engagements for future collaborations discussed |
| Year(s) Of Engagement Activity | 2018 |
| Description | V Nair was invited to talk a the Poultry Health Workshop and Conference on 19th-20th Nov, 2018 in Kathmandu, Nepal |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Public/other audiences |
| Results and Impact | more than 100 stakeholders from different sectors of the Poultry industry and academia attended the meeting. There was engagement from the different sectors for initiating a combined approach for finding solutions |
| Year(s) Of Engagement Activity | 2018 |
| URL | http://www.cmdn.org. |
| Description | Vet School Open Day |
| Form Of Engagement Activity | Participation in an open day or visit at my research institution |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Schools |
| Results and Impact | University of Surrey School of Veterinary Medicine Open Day - introduced The Pirbright Institute and the research we conduct to prospective students and parents. |
| Year(s) Of Engagement Activity | 2018 |
| Description | Vet Times |
| Form Of Engagement Activity | A magazine, newsletter or online publication |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Information on research activity of Avian Immunology group at the Pirbright Institute |
| Year(s) Of Engagement Activity | 2018 |
| Description | poster presentation, University of Liverpool IGH Day, 15th October 2018 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Professional Practitioners |
| Results and Impact | poster presentation, University of Liverpool IGH Day, 15th October 2018 |
| Year(s) Of Engagement Activity | 2018 |